CmaCh01G005240 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G005240
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionFAD-binding Berberine family protein
LocationCma_Chr01 : 2715974 .. 2719534 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTGTAGAGGTTAATTTTAGAAGAAAAAGATGAACACAAATGGGGTAAGCCCAACTAAGAACCAAACTAACAAAACAAAGGAGTTGGTATTGTGAGGGTACCTACCAATATCATATAATTTATTGTAAATATTAAATCCCAATCACCAATTTAGTAAAAAAAATTTAAGGATATTAATAAAACTATTGAAAGCATCCCGGGAGCAAAATCAAGTCTATGCGTGTAATATTGAAAAGGACTCAAAATGTACGGTAGGAGACATGGTGTCAACTCTCCACCACTTTTGAGCATTGTAATCTAAGCGAGCTACCCTGTGATACATGTGTGTGTCATAGTGACGGCGAGCGTGCTGAAACGAGTGTCTCGAGCGACTTCCTTTGAAATGGGTTTTTCAAGAACAGTACCGGGCAACACGGGTGTGCCAATATTGCGCCTTAGCCTGCGTGTCGGTATGCACCCACTATCCAATACGGTATCCGTACATGATATGACATGGGCATAGGAGGACCAATGTATCGATAGAATTCAGATGGATGGATGTTCGGGATGTCTCGATGAAATGAGCGATACGTGGGTCCGTTCTCGATGACATGACCCATTGAGTTGTGATGACTGACGACACCTCAAGACTTGGAATGACGTCAAGACATGAGTCATGTCAATCGGGAACTAAGATGACAAGATGAGATACGATATTGCATTGAGAGATCTTAACCCAGAAGCAAAGGCGAGTCAAATGACTTGGTTTAGTGGAGAGGCAAATTAGTTTTGCAACATAAGTATGTTAATTTCATCTCTGTTCTTCTATCACTTCTATTATGGGGTTCTTGGGGGAGCAATCTTGTAGATTTTCTTCACTGTATTTCTTCCAATTCCTCAAATCCAAATCCAACCCTCTTTTACACTCAATCTTCATCCTCCTACTCCTCTGTTCTAAATTTCACCATTCGAAACCTCAGATTCTCAGGGCCCAATGTCCCTAAGCCAATCGCCATAATCAAACCATCGCAACCTTCCCAAGTTCAAGCAGCCGTCATATGCTGCAACAATCACGACCTCCGCATCCGAGTCCGCAGCGGCGGCCATGACTTCAAGGGTCTTTCTTACGTTGCCAATGTCACATTCGTGGTGATTGATTTGGTCAATCTTAATTAAGCGACGGTGGACATTGAAAACAACACTGCGTGGGTTCAATCTGGGGCGACCCAAGGTGAGGTGTATTACAAACTTGGCCTAAAAACCTCTGCTTTTGGTTTCCCCGGCGGAGTGCAGCCGACTGTGGGAATTGGTGGGTTCCTCAGCGGCGGTGGGTATGGGATGATGGTTAGAAGATATGGGCTTGGTGCAGACAATGTGGTGAATGCTCTACTTGTGGACGCTAATGGGAGGCTTCTTGATAGAGATTCTATGGGGGAGGATTTGTTTTGGGCCATTAGAGGCGGCGGAGGAGGCAGCTTTGGGATTGTCTTGGCATGGAAGTTGAAGTTGGTTCCTGATATGGAAAAATCGCTCGAGAGTAAGATTAGAATTACCTTATTGATTAAATATCTCAAGACAAGAACATTGTTTGAGATTCGAATCACTGGACAAGCAAGATCGATCCTGTCTAGCTTGAATGATTCTTGTTGATCAAATATCTCAACGCAAAAACACTTGTTTGAGATTCAAACCACTCAACAAGCAAGATTGATCATGTCGAGCTTCAATGATTCTACATGCAACCTAAACTACATAGAATTGTAAACAAACTAAGTCATTAGCTAAAGAAACACAAAGTCTCTTTTTACTGTATTTTCCAAATTCACTTAAAAAAAACATACCATTCTTTATATAGCCTCAAAAATGAAACTACTACATTCCAAGGATTTAACATTCATATTTATGACCATAATTAGCCGGGTGTAAATGTAATCTAGAGTAAATAAAAAGTCTTCAAATACAATAACTCTAAACGGTAACCACCCAAAATTTATAACAATCAAACTTCATTCTTCTTCAATATGGCATGAATTGAAACATCTTTTGATAATTTTGACAGTCTTTTCTTCACATCTTCATGGAAGTATATTGCATGATTGATATCTTTTAGTTCATATCAGTTCCGGTTCCTCCTACTGTTACGTCCTTCGTGGCTTATAGAAGTTTGGACCAAAACACTACCGACTTGGTTCATCGATGGCAGTTCGTTGCTCCGGTGATTGATGAAAGATTGTTCATTGGGTCATGACTCTCTACCATGAATAATATCAATGGAGATGTATTGCTTAGTTATAAACCAATGATTAACCCCTTAATTAGTAGATGTGAGACTTCTCTCTCGGGCCCGAGGAAGTCCTTGAACAAAGTACACCATTTGTTCACACTTAGTCACTTTTGACTACACCTTCGAAGCTTATAAACCCATGATCAACCCATTATTTAGTAGATGTAGGACTCCTCTCTAGGGCCTATGGAGCCCTCGAATAAAGTATACTACACCATTTGTTCAACACTTAAGTCACTTTTGACTATACATTCGAGGCTTATAAATCCATGATCATCCCCTTAATTAGTAGATGTGGGACTCCTCTCTAGGGCGGATCCCTCGAACAAAGTAGTACACCCTTTGTTCGACACTTGAGGATTCTACTGAAATGACTAAGTTAAGGGCATGACTTTGATACCATATTAGAAACCACGACTCTCCATAATGGTATGATATTGTCCATTTTGAGCACAAGGACTCGTATCAATGGAGATATATTTCTTACTTATAAACTCGTGATCATTCCATTAATTAGCCAACGTGGGACTCCCTCCCAGCAATCCTCAACACTCTTTCCATGTCTCGCCCACGAACATTTTTACGTGGTTTGAAAGTTCGAAAGTTAGGAGATTTAAATAGAAGAATGCAATTTCTTTTCACAGCTGCAAATTCAATGGAAGGAGGAGGAAGAACAAAGGGAGCATTTTTCTACTCACTGTTTCTAGGAAAGGCAGAGGAGCTTGTACCCATCATGGAGAAGAGCTTCCCAGAGCTGGGACTGAAAAAAGAAGACTGTTTGGAAACCAATTGGGTTGAATCAACGGCATATCCCGCAAGTGGGTTTGTGACAGCAAACGATTTGAATGTCTTACTTGACAGAGCCCCACTCAGCAGCGGCCGATACAAAACCAAATCAGATTACATCACACAACCCATCTCAGAACCCGCCCTCGACGGCATATGGGAAAGGTTGGACGCCGATGGCATAGAGACAGTGCAGCTCGTGCTGATCCGCTACGGAGGGAAAATGAATGAGATATCTGAAACAGAGCTCCCTTCGCCGCATCGAGCAGGGTATCCCATAAAAATTGGCTACTATGTTACATGGGAATCCCCTGAAGCTGACATGAGGCACTTGGAATGGAGCCGAGAGGTGTATGAGTACATGGCTCCTTTCGTGTCCAAATGGCCCAGATCTGCGTATGTCAATTACAGAGACCTTGACATTGGGACAAACAACGAGGACGGGACCCCGACGAGCTTCGAGGAAGCAAGTGTTTGGGGATTAATTTTGAGAGGTTGGTGCGAGTGA

mRNA sequence

ATGGTTTGTAGAGATTTTCTTCACTGTATTTCTTCCAATTCCTCAAATCCAAATCCAACCCTCTTTTACACTCAATCTTCATCCTCCTACTCCTCTGTTCTAAATTTCACCATTCGAAACCTCAGATTCTCAGGGCCCAATGTCCCTAAGCCAATCGCCATAATCAAACCATCGCAACCTTCCCAAGTTCAAGCAGCCGTCATATGCTGCAACAATCACGACCTCCGCATCCGAGTCCGCAGCGGCGGCCATGACTTCAAGGCGACGGTGGACATTGAAAACAACACTGCGTGGGTTCAATCTGGGGCGACCCAAGGTGAGGTGTATTACAAACTTGGCCTAAAAACCTCTGCTTTTGGTTTCCCCGGCGGAGTGCAGCCGACTGTGGGAATTGGTGGGTTCCTCAGCGGCGGTGGGTATGGGATGATGGTTAGAAGATATGGGCTTGCTGCAAATTCAATGGAAGGAGGAGGAAGAACAAAGGGAGCATTTTTCTACTCACTGTTTCTAGGAAAGGCAGAGGAGCTTGTACCCATCATGGAGAAGAGCTTCCCAGAGCTGGGACTGAAAAAAGAAGACTGTTTGGAAACCAATTGGGTTGAATCAACGGCATATCCCGCAAGTGGGTTTGTGACAGCAAACGATTTGAATGTCTTACTTGACAGAGCCCCACTCAGCAGCGGCCGATACAAAACCAAATCAGATTACATCACACAACCCATCTCAGAACCCGCCCTCGACGGCATATGGGAAAGGTTGGACGCCGATGGCATAGAGACAGTGCAGCTCGTGCTGATCCGCTACGGAGGGAAAATGAATGAGATATCTGAAACAGAGCTCCCTTCGCCGCATCGAGCAGGGTATCCCATAAAAATTGGCTACTATGTTACATGGGAATCCCCTGAAGCTGACATGAGGCACTTGGAATGGAGCCGAGAGGTGTATGAGTACATGGCTCCTTTCGTGTCCAAATGGCCCAGATCTGCGTATGTCAATTACAGAGACCTTGACATTGGGACAAACAACGAGGACGGGACCCCGACGAGCTTCGAGGAAGCAAGTGTTTGGGGATTAATTTTGAGAGGTTGGTGCGAGTGA

Coding sequence (CDS)

ATGGTTTGTAGAGATTTTCTTCACTGTATTTCTTCCAATTCCTCAAATCCAAATCCAACCCTCTTTTACACTCAATCTTCATCCTCCTACTCCTCTGTTCTAAATTTCACCATTCGAAACCTCAGATTCTCAGGGCCCAATGTCCCTAAGCCAATCGCCATAATCAAACCATCGCAACCTTCCCAAGTTCAAGCAGCCGTCATATGCTGCAACAATCACGACCTCCGCATCCGAGTCCGCAGCGGCGGCCATGACTTCAAGGCGACGGTGGACATTGAAAACAACACTGCGTGGGTTCAATCTGGGGCGACCCAAGGTGAGGTGTATTACAAACTTGGCCTAAAAACCTCTGCTTTTGGTTTCCCCGGCGGAGTGCAGCCGACTGTGGGAATTGGTGGGTTCCTCAGCGGCGGTGGGTATGGGATGATGGTTAGAAGATATGGGCTTGCTGCAAATTCAATGGAAGGAGGAGGAAGAACAAAGGGAGCATTTTTCTACTCACTGTTTCTAGGAAAGGCAGAGGAGCTTGTACCCATCATGGAGAAGAGCTTCCCAGAGCTGGGACTGAAAAAAGAAGACTGTTTGGAAACCAATTGGGTTGAATCAACGGCATATCCCGCAAGTGGGTTTGTGACAGCAAACGATTTGAATGTCTTACTTGACAGAGCCCCACTCAGCAGCGGCCGATACAAAACCAAATCAGATTACATCACACAACCCATCTCAGAACCCGCCCTCGACGGCATATGGGAAAGGTTGGACGCCGATGGCATAGAGACAGTGCAGCTCGTGCTGATCCGCTACGGAGGGAAAATGAATGAGATATCTGAAACAGAGCTCCCTTCGCCGCATCGAGCAGGGTATCCCATAAAAATTGGCTACTATGTTACATGGGAATCCCCTGAAGCTGACATGAGGCACTTGGAATGGAGCCGAGAGGTGTATGAGTACATGGCTCCTTTCGTGTCCAAATGGCCCAGATCTGCGTATGTCAATTACAGAGACCTTGACATTGGGACAAACAACGAGGACGGGACCCCGACGAGCTTCGAGGAAGCAAGTGTTTGGGGATTAATTTTGAGAGGTTGGTGCGAGTGA

Protein sequence

MVCRDFLHCISSNSSNPNPTLFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQVQAAVICCNNHDLRIRVRSGGHDFKATVDIENNTAWVQSGATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSMEGGGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWGLILRGWCE
BLAST of CmaCh01G005240 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-43
Identity = 90/223 (40.36%), Postives = 134/223 (60.09%), Query Frame = 1

Query: 142 MMVRRYGLAANSMEGGGRTKGA---FFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETN 201
           +++  + +  N  +  G+ K A   +F S+FLG  + LV +M KSFPELG+KK DC + +
Sbjct: 286 LLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLS 345

Query: 202 WVESTAYPASGFVTANDLN----VLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLD 261
           W+++  +  SG V  +  N    +LLDR+   +G +K K DY+ +PI E     I E+L 
Sbjct: 346 WIDTIIF-YSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLY 405

Query: 262 ADGIETVQLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREV 321
            + I      L  YGG M+EISE+ +P PHRAG   ++ Y  +WE  E + +HL W R +
Sbjct: 406 EEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNI 465

Query: 322 YEYMAPFVSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
           Y +M P+VSK PR AY+NYRDLDIG  N+   P ++ +A +WG
Sbjct: 466 YNFMTPYVSKNPRLAYLNYRDLDIGI-NDPKNPNNYTQARIWG 506

BLAST of CmaCh01G005240 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.3e-42
Identity = 89/223 (39.91%), Postives = 132/223 (59.19%), Query Frame = 1

Query: 142 MMVRRYGLAANSMEGGGRTKGA---FFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETN 201
           +M+  +    N  +  G+ K     +F S+FLG  + LV +M KSFPELG+KK DC E +
Sbjct: 287 LMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELS 346

Query: 202 WVESTAYPASGFVTANDLN----VLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLD 261
           W+++T +  SG V  N  N    +LLDR+      +  K DY+ + I E A+  I E+L 
Sbjct: 347 WIDTTIF-YSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLY 406

Query: 262 ADGIETVQLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREV 321
            + +     VL  YGG M+EISE+ +P PHRAG   ++ Y  TWE  E + +H+ W R V
Sbjct: 407 EEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSV 466

Query: 322 YEYMAPFVSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
           Y +  P+VS+ PR AY+NYRDLD+G  N + +P ++ +A +WG
Sbjct: 467 YNFTTPYVSQNPRLAYLNYRDLDLGKTNPE-SPNNYTQARIWG 507

BLAST of CmaCh01G005240 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.7e-42
Identity = 87/223 (39.01%), Postives = 131/223 (58.74%), Query Frame = 1

Query: 142 MMVRRYGLAANSMEGGGRTKGA---FFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETN 201
           +++  + +  N  +  G+ K     +F S+F G  + LV +M KSFPELG+KK DC E +
Sbjct: 287 LVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFS 346

Query: 202 WVESTAYPASGFVTANDLN----VLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLD 261
           W+++T +  SG V  N  N    +LLDR+      +  K DY+ +PI E A+  I E+L 
Sbjct: 347 WIDTTIF-YSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLY 406

Query: 262 ADGIETVQLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREV 321
            + +     VL  YGG M EISE+ +P PHRAG   ++ Y  +WE  E + +H+ W R V
Sbjct: 407 EEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSV 466

Query: 322 YEYMAPFVSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
           Y +  P+VS+ PR AY+NYRDLD+G  N   +P ++ +A +WG
Sbjct: 467 YNFTTPYVSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWG 507

BLAST of CmaCh01G005240 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.8e-41
Identity = 85/216 (39.35%), Postives = 128/216 (59.26%), Query Frame = 1

Query: 149 LAANSMEGGGRTKG---AFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAY 208
           +  N  +  G+ K    ++F S+F G  + LV +M KSFPELG+KK DC + +W+++  +
Sbjct: 294 ITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIF 353

Query: 209 PASGFVTANDLN----VLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETV 268
             SG V  N  N    +LLDR+      +  K DY+ +PI E A+  I E+L  + +   
Sbjct: 354 -YSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVG 413

Query: 269 QLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPF 328
             V   YGG M+EISE+ +P PHRAG   +I Y  +WE  E + +H+ W R VY +  P+
Sbjct: 414 MFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEKQEDNEKHINWIRNVYNFTTPY 473

Query: 329 VSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
           VS+ PR AY+NYRDLD+G  N + +P ++ +A +WG
Sbjct: 474 VSQNPRMAYLNYRDLDLGKTNFE-SPNNYTQARIWG 507

BLAST of CmaCh01G005240 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.2e-40
Identity = 84/216 (38.89%), Postives = 127/216 (58.80%), Query Frame = 1

Query: 149 LAANSMEGGGRTKG---AFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAY 208
           +  N  +  G+ K    ++F S+F G  + LV +M KSFPELG+KK DC + +W+++  +
Sbjct: 294 ITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIF 353

Query: 209 PASGFVTANDL----NVLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETV 268
             SG V  N       +LLDR+      +  K DY+ +PI E A+  I E+L  + +   
Sbjct: 354 -YSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVG 413

Query: 269 QLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPF 328
             V   YGG M+EISE+ +P PHRAG   +I Y  +WE  E + +H+ W R VY +  P+
Sbjct: 414 MFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEKQEDNEKHINWIRNVYNFTTPY 473

Query: 329 VSKWPRSAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
           VS+ PR AY+NYRDLD+G  N + +P ++ +A +WG
Sbjct: 474 VSQNPRMAYLNYRDLDLGKTNFE-SPNNYTQARIWG 507

BLAST of CmaCh01G005240 vs. TrEMBL
Match: A0A151RZI8_CAJCA (Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_030436 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.1e-80
Identity = 177/436 (40.60%), Postives = 241/436 (55.28%), Query Frame = 1

Query: 5   DFLHCISSNSSNPNPT--LFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           +F+ C+ +   N      + YTQ++SSY S L+F+I+NLRF   +  KP+ I+ P   S 
Sbjct: 29  NFVQCLYNYPHNTTSISKVVYTQTNSSYLSTLDFSIQNLRFYNVS-SKPLVIVTPLDVSH 88

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           +QA +IC  +H L+IR RSGGHD++                      VD+EN+TAWVQ+G
Sbjct: 89  IQATIICSQHHGLQIRTRSGGHDYEGLSYVAEVPFVVVDLINLREINVDVENSTAWVQAG 148

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSM-------- 184
           AT GE+Y+ +  K+   GFP GV P VG GG  SGGGYG ++RRYGLAA+++        
Sbjct: 149 ATIGELYHSISEKSKTLGFPAGVCPPVGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDV 208

Query: 185 -------------------EGGGRTKG------------------------------AFF 244
                               GGG + G                              A F
Sbjct: 209 KGNVLDRAGMGEDLFWAIRGGGGASFGVIVAWKIKLVSVPSTVTVVNSSQNGKLTVEAQF 268

Query: 245 YSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPL 304
            S++LG  +EL+P+M+ SFPELGL +EDC E +W++S  Y A GF +    +VLL+R  L
Sbjct: 269 ESMYLGGVDELIPLMQNSFPELGLVREDCTEMSWIDSVLYMA-GFTSGQSTDVLLNRTQL 328

Query: 305 SS-GRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPH 359
           +    +K KSDY+  PI +  L G+W     D  +   +    YGG+M+EISE+E+P PH
Sbjct: 329 NGLSFFKAKSDYVRDPIPDVGLQGLWPFFYEDEAQAAYVQFTPYGGRMDEISESEIPFPH 388

BLAST of CmaCh01G005240 vs. TrEMBL
Match: A0A151S4C8_CAJCA (Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_028595 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 2.4e-80
Identity = 175/431 (40.60%), Postives = 237/431 (54.99%), Query Frame = 1

Query: 5   DFLHCISSNSSNPNPT--LFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           +F+ C+ +   N      +  TQ++SSYSS+L F+I+N RFS  +  KP+ I+ P   S 
Sbjct: 33  NFVQCLYNYPQNATSISKVVNTQANSSYSSILQFSIQNPRFSNAS-SKPLVIVTPLAVSH 92

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           +QA +IC   H L+IR RSGGHD++                      VD+EN+TAWVQ+G
Sbjct: 93  IQATIICSQRHGLQIRTRSGGHDYEGLSYVAKVPFVLIDLINLREINVDVENSTAWVQAG 152

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSM-------- 184
           AT GE+YY +  K++  GFPGGV P++G GG  SGGGYG ++RRYGLAA+++        
Sbjct: 153 ATVGELYYSISQKSTTLGFPGGVWPSMGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDV 212

Query: 185 -------------------EGGGRTKG--------------------------AFFYSLF 244
                               GGG + G                          A F S++
Sbjct: 213 KGNVLDREGMGEDLFWAIRGGGGASFGVIVAWKLKLVPVPSTVTVQSGKPTVQAIFESMY 272

Query: 245 LGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPLSSGR 304
           LG  +EL+P+M+KSFPELGL ++DC E +W++S  Y A    +      LL+R  +    
Sbjct: 273 LGGVDELIPLMQKSFPELGLVRQDCTEMSWIDSVLYIARS-TSGQPREALLNRTGIGLPF 332

Query: 305 YKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPHRAGYP 359
           +K KSDY+  PI    L G+W     D      +    YGGKM+EISE+E+P PHR+G  
Sbjct: 333 FKAKSDYVRDPIPVVGLQGLWSLFYEDEALAAFIQFTPYGGKMDEISESEIPFPHRSGNI 392

BLAST of CmaCh01G005240 vs. TrEMBL
Match: A0A0A0LS77_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G096090 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.5e-79
Identity = 136/195 (69.74%), Postives = 165/195 (84.62%), Query Frame = 1

Query: 163 AFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDR 222
           A F+SLFLG A EL+ +MEK+FPELGLKKEDCLET+WVES A+ ASGFV+A  L +LLDR
Sbjct: 3   ASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDR 62

Query: 223 APLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPS 282
            PL +GRYKTKSDY T+PISE  L+G+WER   + +ETVQL+LI +GGK NEISE+E PS
Sbjct: 63  TPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESETPS 122

Query: 283 PHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNYRDLDIGTNN 342
           PHRAGYPI IGYY+TW+ P+AD +HL+W+RE++ YM PFVSK PR+AYVNYRDLD+GTNN
Sbjct: 123 PHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNN 182

Query: 343 EDGTPTSFEEASVWG 358
           +DG PT  EEAS+WG
Sbjct: 183 DDGVPTRCEEASIWG 197

BLAST of CmaCh01G005240 vs. TrEMBL
Match: M5WQ23_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021171mg PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-76
Identity = 160/386 (41.45%), Postives = 231/386 (59.84%), Query Frame = 1

Query: 2   VCRDFLHCISSN--SSNPNPTLFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQ 61
           +    L C+S++   SN +  +  T++SS+YSS+L  +I+NLRF   + PKP  I+ P  
Sbjct: 27  ISESLLQCLSTHLKHSNSSKEIILTRNSSAYSSILQSSIQNLRFLNNSTPKPKVIVTPFH 86

Query: 62  PSQVQAAVICCNNHDLRIRVRSGGHDFKAT--------------------VDIENNTAWV 121
            S VQAAVIC   H ++ R +SGGHD++                      +D++N +AWV
Sbjct: 87  ESHVQAAVICSKKHGIQKRFQSGGHDYEGLSYVSYAPFIIIELFNLQTIHIDVKNESAWV 146

Query: 122 QSGATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSMEGGGR 181
           +SGAT GE+YY +  K++   FP G  PT+G+GG +SGGG+G + R+YGLA +++     
Sbjct: 147 ESGATLGELYYLIAQKSNVHEFPAGSCPTMGVGGHISGGGFGTIFRKYGLAVDNVIDARI 206

Query: 182 TKGAFFYSLFL-------GKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVT 241
                   LFL        +AE L+ +M+  FP+L L   +C E +W++S  Y A GF  
Sbjct: 207 VD--LHEDLFLHLVVEVANEAEALL-LMQDKFPDLSLDHSNCTEMSWIQSVMYFA-GFPI 266

Query: 242 ANDLNVLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKM 301
           +  L VLL R   S   +K KSD +TQPIS+  L+G+W+RL    +ET QL+L  YGG+M
Sbjct: 267 SEYLEVLLKRTQPSRSFFKAKSDNVTQPISQAGLEGLWQRLLE--VETSQLILTPYGGRM 326

Query: 302 NEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVN 359
           +EIS++E P PHR G    I Y VTW+  +   +H+ W R VY YMA  VSK PR+ Y+N
Sbjct: 327 SEISDSETPFPHRNGSIFAIQYLVTWDDDKETEKHISWMRRVYAYMASHVSKSPRAVYLN 386

BLAST of CmaCh01G005240 vs. TrEMBL
Match: A0A067KWX9_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09769 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.4e-72
Identity = 159/379 (41.95%), Postives = 217/379 (57.26%), Query Frame = 1

Query: 5   DFLHCIS--SNSSNPNPTLFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           DFL C+   S  S+    + YT  +SSY SVL   I N RF+    PKP  I+ P   S 
Sbjct: 31  DFLECLKLQSEDSSSISKVIYTPINSSYLSVLQLGINNERFNTSTTPKPQVIVTPLNLSH 90

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           ++A + C   HDL+IR+RSGGHD++                     TVD  N TAWVQ G
Sbjct: 91  IEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFVILDLITLQSITVDATNKTAWVQVG 150

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSMEGGGRTKG 184
           AT G++YY +  K+    FP  V  TVGIGG  SGGGYG ++R+YGLAA++         
Sbjct: 151 ATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGGGYGTLLRKYGLAADN--------- 210

Query: 185 AFFYSLFLGKAEELVPIMEKSFPEL-GLKKEDCLETNWVESTAYPASGFVTANDLNVLLD 244
                           I++    ++ GL KED  E +W+ES  Y A GF + + L +LL+
Sbjct: 211 ----------------IIDAQLTDVDGLTKEDSTEMSWIESVLYFA-GF-SNSSLEILLE 270

Query: 245 RAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELP 304
           R   +   +K KSDY+ QP+ E AL+GIW+RL    + + QL+   YGG+++EISE+ +P
Sbjct: 271 RTQQNVRYFKAKSDYVQQPMPEVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIP 330

Query: 305 SPHRAGYPIKIGYYVTW--ESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNYRDLDIG 359
            PHRAG   KI + V W  E  E  + H+ W R +Y ++AP+VSK PRSAY+NYRDLDIG
Sbjct: 331 FPHRAGNLYKIQHSVYWNDEGIEESITHISWIRRLYSFLAPYVSKNPRSAYINYRDLDIG 380

BLAST of CmaCh01G005240 vs. TAIR10
Match: AT5G44400.1 (AT5G44400.1 FAD-binding Berberine family protein)

HSP 1 Score: 176.0 bits (445), Expect = 4.2e-44
Identity = 90/204 (44.12%), Postives = 127/204 (62.25%), Query Frame = 1

Query: 156 GGGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTAND 215
           GG +T    + +LFLG    L+ +M+KSFPELGL  +DC+E +W+ES AY  SGF T   
Sbjct: 304 GGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY-ISGFPTHTP 363

Query: 216 LNVLLD-RAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNE 275
            NVLL  ++P     +K KSD++  PI E  L GI+++L  + I    ++   YGG M +
Sbjct: 364 TNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAK 423

Query: 276 ISETELPSPHRAGYPIKIGYYVTW-ESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNY 335
           I E+++P PHR G   K+ Y  +W +S +   RH+ W R++Y YM P+VS  PR AYVNY
Sbjct: 424 IPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNY 483

Query: 336 RDLDIGTNNEDGTPTSFEEASVWG 358
           RDLD+G N +D   T  ++A VWG
Sbjct: 484 RDLDLGRNTKD-VKTCIKQAQVWG 503

BLAST of CmaCh01G005240 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 168.3 bits (425), Expect = 8.7e-42
Identity = 85/200 (42.50%), Postives = 120/200 (60.00%), Query Frame = 1

Query: 159 RTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNV 218
           RT    +   FLG A  L+ +M++SFP+LGL K+DCLET+W++S  Y A GF +      
Sbjct: 309 RTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEA 368

Query: 219 LLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISET 278
           LLD   L    +K KSDY+ +PI    L+G+WE+L  +  ++   +   YGG M +I ET
Sbjct: 369 LLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEE--DSPLTIWNPYGGMMAKIPET 428

Query: 279 ELPSPHRAGYPIKIGYYVTWESPE-ADMRHLEWSREVYEYMAPFVSKWPRSAYVNYRDLD 338
           E P PHR+G   KI +   W+  + ++ +H+ W RE+Y YM  +VSK PRSAYVNYRDLD
Sbjct: 429 ETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLD 488

Query: 339 IGTNNEDGTPTSFEEASVWG 358
           +G N +        +A  WG
Sbjct: 489 LGMNGKG------SDAREWG 499

BLAST of CmaCh01G005240 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 168.3 bits (425), Expect = 8.7e-42
Identity = 89/210 (42.38%), Postives = 126/210 (60.00%), Query Frame = 1

Query: 150 AANSMEGGGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASG 209
           A+ + + G RT    + + FLG +  L+ +M+KSFPELGL K+DC E +W++S  Y A G
Sbjct: 298 ASKTTKPGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-G 357

Query: 210 FVTANDLNVLLDRAPLSSGRYKTKSDYITQPISEPALDGIWER-LDADGIETVQLVLIRY 269
           F  +     LL    L    +K KSD++ +PI    L+G+WER L+ D   T+      Y
Sbjct: 358 FPNSAAPEALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNP---Y 417

Query: 270 GGKMNEISETELPSPHRAGYPIKIGYYVTWESPE-ADMRHLEWSREVYEYMAPFVSKWPR 329
           GG M+ ISE+E+P PHR G   KI +  TW+  + ++ RH++W RE+Y YM  +VSK PR
Sbjct: 418 GGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPR 477

Query: 330 SAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
            AYVNYRDLD+GTN  +       +A  WG
Sbjct: 478 QAYVNYRDLDLGTNEGE------TDAREWG 497

BLAST of CmaCh01G005240 vs. TAIR10
Match: AT4G20860.1 (AT4G20860.1 FAD-binding Berberine family protein)

HSP 1 Score: 166.0 bits (419), Expect = 4.3e-41
Identity = 87/210 (41.43%), Postives = 128/210 (60.95%), Query Frame = 1

Query: 152 NSMEGGGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFV 211
           NS+EG  R   + F +LFLG  + L+P+M + FPELGL+ +DC E +W+ES  +    + 
Sbjct: 294 NSLEGNQRKVKSTFQTLFLGGIDRLIPLMNQKFPELGLRSQDCSEMSWIESIMF--FNWR 353

Query: 212 TANDLNVLLDRAPLSSGRY-KTKSDYITQPISEPALDGIWER-LDADGIETVQLVLIRYG 271
           +   L +LL+R      +Y K KSDY+ +P+ E   + + +R L+ D   T  ++    G
Sbjct: 354 SGQPLEILLNRDLRFEDQYFKAKSDYVQKPVPENVFEEVTKRFLEQD---TPLMIFEPLG 413

Query: 272 GKMNEISETELPSPHRAGYPIKIGYYVTWESPEAD--MRHLEWSREVYEYMAPFVSKWPR 331
           GK+++ISETE P PHR G    I Y V W+  E +   +H+ W R +++YM P+VSK PR
Sbjct: 414 GKISKISETESPYPHRRGNLYNIQYMVKWKVNEVEEMNKHVRWMRSLHDYMTPYVSKSPR 473

Query: 332 SAYVNYRDLDIGTNNEDGTPTSFEEASVWG 358
            AY+NYRDLD+G+    G  TSFE+A  WG
Sbjct: 474 GAYLNYRDLDLGSTK--GINTSFEDARKWG 496

BLAST of CmaCh01G005240 vs. TAIR10
Match: AT5G44380.1 (AT5G44380.1 FAD-binding Berberine family protein)

HSP 1 Score: 164.9 bits (416), Expect = 9.6e-41
Identity = 85/203 (41.87%), Postives = 123/203 (60.59%), Query Frame = 1

Query: 157 GGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDL 216
           G +T    +   FLG+   L+ +MEK+FPELGL ++DC E +W+E+  +   GF T + +
Sbjct: 308 GNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG-GFPTGSPI 367

Query: 217 NVLLD-RAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEI 276
            +LL  ++PL    +K  SD++ +PI    L GI++RL  +G  T  L    YGG M++I
Sbjct: 368 EILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRL-IEG-NTTFLNWTPYGGMMSKI 427

Query: 277 SETELPSPHRAGYPIKIGYYVTW-ESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNYR 336
            E+ +P PHR G   KI YY  W E+ +   R + W +E+Y YMAP+VS  PR AYVNYR
Sbjct: 428 PESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYR 487

Query: 337 DLDIGTNNEDGTPTSFEEASVWG 358
           DLD G  N++    +F EA +WG
Sbjct: 488 DLDFG-QNKNNAKVNFIEAKIWG 506

BLAST of CmaCh01G005240 vs. NCBI nr
Match: gi|449459146|ref|XP_004147307.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 310.8 bits (795), Expect = 3.1e-81
Identity = 140/206 (67.96%), Postives = 171/206 (83.01%), Query Frame = 1

Query: 152 NSMEGGGRTKGAFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFV 211
           +S +G GR   A F+SLFLG A EL+ +MEK+FPELGLKKEDCLET+WVES A+ ASGFV
Sbjct: 268 SSHDGSGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFV 327

Query: 212 TANDLNVLLDRAPLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGK 271
           +A  L +LLDR PL +GRYKTKSDY T+PISE  L+G+WER   + +ETVQL+LI +GGK
Sbjct: 328 SAKSLELLLDRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGK 387

Query: 272 MNEISETELPSPHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKWPRSAYV 331
            NEISE+E PSPHRAGYPI IGYY+TW+ P+AD +HL+W+RE++ YM PFVSK PR+AYV
Sbjct: 388 TNEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYV 447

Query: 332 NYRDLDIGTNNEDGTPTSFEEASVWG 358
           NYRDLD+GTNN+DG PT  EEAS+WG
Sbjct: 448 NYRDLDMGTNNDDGVPTRCEEASIWG 473

BLAST of CmaCh01G005240 vs. NCBI nr
Match: gi|1012336654|gb|KYP47949.1| (Reticuline oxidase-like protein [Cajanus cajan])

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-80
Identity = 177/436 (40.60%), Postives = 241/436 (55.28%), Query Frame = 1

Query: 5   DFLHCISSNSSNPNPT--LFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           +F+ C+ +   N      + YTQ++SSY S L+F+I+NLRF   +  KP+ I+ P   S 
Sbjct: 29  NFVQCLYNYPHNTTSISKVVYTQTNSSYLSTLDFSIQNLRFYNVS-SKPLVIVTPLDVSH 88

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           +QA +IC  +H L+IR RSGGHD++                      VD+EN+TAWVQ+G
Sbjct: 89  IQATIICSQHHGLQIRTRSGGHDYEGLSYVAEVPFVVVDLINLREINVDVENSTAWVQAG 148

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSM-------- 184
           AT GE+Y+ +  K+   GFP GV P VG GG  SGGGYG ++RRYGLAA+++        
Sbjct: 149 ATIGELYHSISEKSKTLGFPAGVCPPVGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDV 208

Query: 185 -------------------EGGGRTKG------------------------------AFF 244
                               GGG + G                              A F
Sbjct: 209 KGNVLDRAGMGEDLFWAIRGGGGASFGVIVAWKIKLVSVPSTVTVVNSSQNGKLTVEAQF 268

Query: 245 YSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPL 304
            S++LG  +EL+P+M+ SFPELGL +EDC E +W++S  Y A GF +    +VLL+R  L
Sbjct: 269 ESMYLGGVDELIPLMQNSFPELGLVREDCTEMSWIDSVLYMA-GFTSGQSTDVLLNRTQL 328

Query: 305 SS-GRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPH 359
           +    +K KSDY+  PI +  L G+W     D  +   +    YGG+M+EISE+E+P PH
Sbjct: 329 NGLSFFKAKSDYVRDPIPDVGLQGLWPFFYEDEAQAAYVQFTPYGGRMDEISESEIPFPH 388

BLAST of CmaCh01G005240 vs. NCBI nr
Match: gi|1012338370|gb|KYP49624.1| (Reticuline oxidase-like protein [Cajanus cajan])

HSP 1 Score: 307.4 bits (786), Expect = 3.4e-80
Identity = 175/431 (40.60%), Postives = 237/431 (54.99%), Query Frame = 1

Query: 5   DFLHCISSNSSNPNPT--LFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           +F+ C+ +   N      +  TQ++SSYSS+L F+I+N RFS  +  KP+ I+ P   S 
Sbjct: 33  NFVQCLYNYPQNATSISKVVNTQANSSYSSILQFSIQNPRFSNAS-SKPLVIVTPLAVSH 92

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           +QA +IC   H L+IR RSGGHD++                      VD+EN+TAWVQ+G
Sbjct: 93  IQATIICSQRHGLQIRTRSGGHDYEGLSYVAKVPFVLIDLINLREINVDVENSTAWVQAG 152

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSM-------- 184
           AT GE+YY +  K++  GFPGGV P++G GG  SGGGYG ++RRYGLAA+++        
Sbjct: 153 ATVGELYYSISQKSTTLGFPGGVWPSMGTGGHFSGGGYGFLMRRYGLAADNIIDAHIIDV 212

Query: 185 -------------------EGGGRTKG--------------------------AFFYSLF 244
                               GGG + G                          A F S++
Sbjct: 213 KGNVLDREGMGEDLFWAIRGGGGASFGVIVAWKLKLVPVPSTVTVQSGKPTVQAIFESMY 272

Query: 245 LGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPLSSGR 304
           LG  +EL+P+M+KSFPELGL ++DC E +W++S  Y A    +      LL+R  +    
Sbjct: 273 LGGVDELIPLMQKSFPELGLVRQDCTEMSWIDSVLYIARS-TSGQPREALLNRTGIGLPF 332

Query: 305 YKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPHRAGYP 359
           +K KSDY+  PI    L G+W     D      +    YGGKM+EISE+E+P PHR+G  
Sbjct: 333 FKAKSDYVRDPIPVVGLQGLWSLFYEDEALAAFIQFTPYGGKMDEISESEIPFPHRSGNI 392

BLAST of CmaCh01G005240 vs. NCBI nr
Match: gi|802609643|ref|XP_012074138.1| (PREDICTED: cannabidiolic acid synthase-like 1 [Jatropha curcas])

HSP 1 Score: 306.2 bits (783), Expect = 7.6e-80
Identity = 174/418 (41.63%), Postives = 235/418 (56.22%), Query Frame = 1

Query: 5   DFLHCIS--SNSSNPNPTLFYTQSSSSYSSVLNFTIRNLRFSGPNVPKPIAIIKPSQPSQ 64
           DFL C+   S  S+    + YT  +SSY SVL   I N RF+    PKP  I+ P   S 
Sbjct: 31  DFLECLKLQSEDSSSISKVIYTPINSSYLSVLQLGINNERFNTSTTPKPQVIVTPLNLSH 90

Query: 65  VQAAVICCNNHDLRIRVRSGGHDFKA--------------------TVDIENNTAWVQSG 124
           ++A + C   HDL+IR+RSGGHD++                     TVD  N TAWVQ G
Sbjct: 91  IEATITCSKKHDLQIRIRSGGHDYEGLSYVSPNPFVILDLITLQSITVDATNKTAWVQVG 150

Query: 125 ATQGEVYYKLGLKTSAFGFPGGVQPTVGIGGFLSGGGYGMMVRRYGLAANSM-------- 184
           AT G++YY +  K+    FP  V  TVGIGG  SGGGYG ++R+YGLAA+++        
Sbjct: 151 ATTGKLYYSIAEKSQNLAFPASVCTTVGIGGHFSGGGYGTLLRKYGLAADNIIDAQLTDV 210

Query: 185 --------------------------------EGGGRTKGAFFYSLFLGKAEELVPIMEK 244
                                            G   T  A F SLFLG+ + L+ +M++
Sbjct: 211 DGRILDRHKWQYVAHKFNEDLFIRVIIERVNSSGNTTTIRAAFKSLFLGRVDRLLTLMQE 270

Query: 245 SFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDRAPLSSGRYKTKSDYITQPIS 304
           SFPELGL KED  E +W+ES  Y A GF  ++ L +LL+R   +   +K KSDY+ QP+ 
Sbjct: 271 SFPELGLTKEDSTEMSWIESVLYFA-GFSNSS-LEILLERTQQNVRYFKAKSDYVQQPMP 330

Query: 305 EPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPSPHRAGYPIKIGYYVTW--ES 359
           E AL+GIW+RL    + + QL+   YGG+++EISE+ +P PHRAG   KI + V W  E 
Sbjct: 331 EVALEGIWKRLFEVEVGSGQLMFNLYGGRVSEISESSIPFPHRAGNLYKIQHSVYWNDEG 390

BLAST of CmaCh01G005240 vs. NCBI nr
Match: gi|700209679|gb|KGN64775.1| (hypothetical protein Csa_1G096090 [Cucumis sativus])

HSP 1 Score: 304.7 bits (779), Expect = 2.2e-79
Identity = 136/195 (69.74%), Postives = 165/195 (84.62%), Query Frame = 1

Query: 163 AFFYSLFLGKAEELVPIMEKSFPELGLKKEDCLETNWVESTAYPASGFVTANDLNVLLDR 222
           A F+SLFLG A EL+ +MEK+FPELGLKKEDCLET+WVES A+ ASGFV+A  L +LLDR
Sbjct: 3   ASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDR 62

Query: 223 APLSSGRYKTKSDYITQPISEPALDGIWERLDADGIETVQLVLIRYGGKMNEISETELPS 282
            PL +GRYKTKSDY T+PISE  L+G+WER   + +ETVQL+LI +GGK NEISE+E PS
Sbjct: 63  TPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISESETPS 122

Query: 283 PHRAGYPIKIGYYVTWESPEADMRHLEWSREVYEYMAPFVSKWPRSAYVNYRDLDIGTNN 342
           PHRAGYPI IGYY+TW+ P+AD +HL+W+RE++ YM PFVSK PR+AYVNYRDLD+GTNN
Sbjct: 123 PHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNN 182

Query: 343 EDGTPTSFEEASVWG 358
           +DG PT  EEAS+WG
Sbjct: 183 DDGVPTRCEEASIWG 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CBDAS_CANSA1.5e-4340.36Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1[more]
THCAI_CANSA1.3e-4239.91Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1[more]
THCAS_CANSA3.7e-4239.01Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1[more]
CASL2_CANSA1.8e-4139.35Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1[more]
CASL1_CANSA1.2e-4038.89Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A151RZI8_CAJCA1.1e-8040.60Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_030436 PE=4 SV=1[more]
A0A151S4C8_CAJCA2.4e-8040.60Reticuline oxidase-like protein OS=Cajanus cajan GN=KK1_028595 PE=4 SV=1[more]
A0A0A0LS77_CUCSA1.5e-7969.74Uncharacterized protein OS=Cucumis sativus GN=Csa_1G096090 PE=4 SV=1[more]
M5WQ23_PRUPE1.6e-7641.45Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa021171mg PE=4 SV=1[more]
A0A067KWX9_JATCU2.4e-7241.95Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09769 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G44400.14.2e-4444.12 FAD-binding Berberine family protein[more]
AT1G30760.18.7e-4242.50 FAD-binding Berberine family protein[more]
AT2G34790.18.7e-4242.38 FAD-binding Berberine family protein[more]
AT4G20860.14.3e-4141.43 FAD-binding Berberine family protein[more]
AT5G44380.19.6e-4141.87 FAD-binding Berberine family protein[more]
Match NameE-valueIdentityDescription
gi|449459146|ref|XP_004147307.1|3.1e-8167.96PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus][more]
gi|1012336654|gb|KYP47949.1|1.5e-8040.60Reticuline oxidase-like protein [Cajanus cajan][more]
gi|1012338370|gb|KYP49624.1|3.4e-8040.60Reticuline oxidase-like protein [Cajanus cajan][more]
gi|802609643|ref|XP_012074138.1|7.6e-8041.63PREDICTED: cannabidiolic acid synthase-like 1 [Jatropha curcas][more]
gi|700209679|gb|KGN64775.1|2.2e-7969.74hypothetical protein Csa_1G096090 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006094Oxid_FAD_bind_N
IPR016166FAD-bd_2
IPR016167FAD-bd_2_sub1
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0050660flavin adenine dinucleotide binding
GO:0016614oxidoreductase activity, acting on CH-OH group of donors
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G005240.1CmaCh01G005240.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 89..154
score: 4.2
IPR016166FAD-binding, type 2unknownSSF56176FAD-binding/transporter-associated domain-likecoord: 35..154
score: 7.48
IPR016167FAD-binding, type 2, subdomain 1GENE3DG3DSA:3.30.43.10coord: 50..90
score: 1.
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2GENE3DG3DSA:3.30.465.10coord: 91..154
score: 3.
NoneNo IPR availablePANTHERPTHR32448FAMILY NOT NAMEDcoord: 5..358
score: 4.0E