CmaCh13G002080 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh13G002080 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 4.4e-140
Identity = 253/537 (47.11%), Postives = 351/537 (65.36%), Query Frame = 1

Query: 7   NFSFISLLLLSFASWGFG---SDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNF 66
           +F F+  ++  F S+      ++  E+FL C S H  N  +N  L++T     Y  +LN 
Sbjct: 7   SFWFVCKIIFFFLSFHIQISIANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNS 66

Query: 67  SIRNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIP 126
           +I+NLRF    TPKP+ IVTP   S +QAT++C K  GLQIRTRSGGHD EG SY+S +P
Sbjct: 67  TIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVP 126

Query: 127 FVLIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFS 186
           FV++DL N++SI IDV  +TAW ++GAT+GE+YY I EK+  L+FP G   ++G+GG FS
Sbjct: 127 FVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFS 186

Query: 187 GGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKL 246
           GGG G L+R YGLA DN++DA+LV+ +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK+
Sbjct: 187 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 246

Query: 247 KLVRVPPTVTSFAVHKSWDLNA-AKLIHWWQYVALEVDEDLFITARVTASNSSKEGGR-- 306
           KLV VP   T F+V K+ +++   KL + WQ +A + D+DL +       N +   G+  
Sbjct: 247 KLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNK 306

Query: 307 -IMKASFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAF-SASEYVSPENL- 366
             +   F S+F G    LV LM K+FPEL +KK DC E SW++   F S     +  N  
Sbjct: 307 TTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFK 366

Query: 367 -ELLLDRTSLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEI 426
            E+LLDR++     + +K DY  KPI ETA+  I E+   E++     +L P+GG M EI
Sbjct: 367 KEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEI 426

Query: 427 SETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKD 486
           SE+  P PHRA     + Y  +W + + + +H+ W R ++++ TP+VS++PR AY+NY+D
Sbjct: 427 SESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRD 486

Query: 487 LDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAP 534
           LD+GK N    P +Y +A +WG +YFG NF RLV+VKTKVDP NFFR+EQSIPP  P
Sbjct: 487 LDLGKTNH-ASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of CmaCh13G002080 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.1e-138
Identity = 247/537 (46.00%), Postives = 350/537 (65.18%), Query Frame = 1

Query: 7   NFSFISLLLLSFASWGFG---SDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNF 66
           +F F+  ++  F S+      ++  E+FL C S +  N  +N   I+T     Y  VLN 
Sbjct: 7   SFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNS 66

Query: 67  SIRNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIP 126
           +I+NLRF    TPKP+ IVTP   S +QA+++C K  GLQIRTRSGGHD EG SY+S +P
Sbjct: 67  TIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVP 126

Query: 127 FVLIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFS 186
           F ++DL N++++ +D+  +TAW ++GAT+GE+YY I E +   +FP G   ++G+GG FS
Sbjct: 127 FAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFS 186

Query: 187 GGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKL 246
           GGG G L+R YGLA DN++DA+LV+ +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK+
Sbjct: 187 GGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKI 246

Query: 247 KLVRVPPTVTSFAVHKSWDLNA-AKLIHWWQYVALEVDEDLFITARVTASNSSKEGGR-- 306
           KLV VP   T F+V K+ +++   KL + WQ +A + D+DL +T      N +   G+  
Sbjct: 247 KLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNK 306

Query: 307 -IMKASFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAF-SASEYVSPENL- 366
             +   F S+FLG    LV LM K+FPEL +KK DC E+SW++   F S     +  N  
Sbjct: 307 TTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFK 366

Query: 367 -ELLLDRTSLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEI 426
            E+LLDR++     + +K DY  K I ETA+  I E+   EE+     +L P+GG M+EI
Sbjct: 367 KEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEI 426

Query: 427 SETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKD 486
           SE+  P PHRA     + Y  TW + + + +H+ W R ++++ TP+VS++PR AY+NY+D
Sbjct: 427 SESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRD 486

Query: 487 LDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAP 534
           LD+GK N +  P +Y +A +WG +YFG NF RLV+VKTK DP NFFR+EQSIPP  P
Sbjct: 487 LDLGKTNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of CmaCh13G002080 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.7e-137
Identity = 239/509 (46.95%), Postives = 340/509 (66.80%), Query Frame = 1

Query: 29  EDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQAS 88
           E+FL C+S +     +N+ L++T     Y  +LN +++NLRF    TPKP+ I TP   S
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 89  QVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWAQS 148
            +Q T++C K  GLQIRTRSGGHD EG SY+S +PFV++DL N++S+ IDV  +TAW +S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 149 GATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYLVD 208
           GAT+GE+YY I E +  L+FPAG   ++G GG FSGGG G L+R YGLA DN++DA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 209 ANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLN-AAK 268
            +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK++LV VP   T F+V K+ +++   K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 269 LIHWWQYVALEVDEDLFITARVTASNSSKEGGR---IMKASFFSLFLGKAAELVSLMEKA 328
           L++ WQ +A   +++L +       N +   G+    + + F S+F G    LV LM K+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 329 FPELELKKEDCLEISWVEAMAF-SASEYVSPENL--ELLLDRTSLITGRYKMKSDYATKP 388
           FPEL +KK DC ++SW++ + F S     +  N   E+LLDR+      + +K DY  KP
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 389 ISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHR 448
           I ETA+  I E+   E++     +  P+GG M+EISE+  P PHRA     I Y  +W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 449 PDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRY 508
            + + +H+ W R ++++ TP+VS++PR AY+NY+DLD+GK N +  P +Y +A +WG +Y
Sbjct: 452 QEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKY 511

Query: 509 FGINFERLVEVKTKVDPFNFFRHEQSIPP 531
           FG NF RLV+VKTKVDP NFFR+EQSIPP
Sbjct: 512 FGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539

BLAST of CmaCh13G002080 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 7.8e-137
Identity = 237/509 (46.56%), Postives = 340/509 (66.80%), Query Frame = 1

Query: 29  EDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQAS 88
           E+FL C S +     +N+ L++T     Y  +LN +I+NLRF    TPKP+ I+TP   S
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 89  QVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWAQS 148
            +Q T++C K  GLQIRTRSGGHD EG SY+S +PFV++DL N++S+ IDV  +TAW ++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 149 GATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYLVD 208
           GAT+GE+YY I E +  L+FPAG   ++G GG FSGGG G L+R YGLA DN++DA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 209 ANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLN-AAK 268
            +GK+LDR SMGEDLFWAIRGGGG +FGI+ AWK++LV VP   T F+V K+ +++   K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 269 LIHWWQYVALEVDEDLFITARVTASNSSKEGGR---IMKASFFSLFLGKAAELVSLMEKA 328
           L++ WQ +A   +++L +       N +   G+    + + F S+F G    LV LM K+
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 329 FPELELKKEDCLEISWVEAMAFSA---SEYVSPENLELLLDRTSLITGRYKMKSDYATKP 388
           FPEL +KK DC ++SW++ + F +   +   +    E+LLDR+      + +K DY  KP
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 389 ISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHR 448
           I ETA+  I E+   E++     +  P+GG M+EISE+  P PHRA     I Y  +W +
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 449 PDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRY 508
            + + +H+ W R ++++ TP+VS++PR AY+NY+DLD+GK N +  P +Y +A +WG +Y
Sbjct: 452 QEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGEKY 511

Query: 509 FGINFERLVEVKTKVDPFNFFRHEQSIPP 531
           FG NF RLV+VKTKVDP NFFR+EQSIPP
Sbjct: 512 FGKNFNRLVKVKTKVDPDNFFRNEQSIPP 539

BLAST of CmaCh13G002080 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 8.6e-136
Identity = 248/540 (45.93%), Postives = 353/540 (65.37%), Query Frame = 1

Query: 1   MKIYWSNFSFISLLLLSFASWGFGS---DNLEDFLHCISLHSSNRHSNSTLIHTPSSSSY 60
           MK    +F F+  ++  F S+   +   +  E+FL C S +  N  +N  L++T ++  Y
Sbjct: 1   MKCSTFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLY 60

Query: 61  PYVLNFSIRNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRS 120
             VLN +I NLRF    TPKP+ IVTP   S +Q T++C K  GLQIRTRSGGHD EG S
Sbjct: 61  MSVLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMS 120

Query: 121 YVSNIPFVLIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIG 180
           Y+S +PFV++DL N+ SI IDV  +TAW ++GAT+GE+YY + EK+  L+  AG   ++ 
Sbjct: 121 YISQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVC 180

Query: 181 LGGFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGI 240
            GG F GGG G L+R YGLA DN++DA+LV+ +GK+LDR SMGEDLFWA+RGGG  SFGI
Sbjct: 181 AGGHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGI 240

Query: 241 VLAWKLKLVRVPPTVTSFAVHKSWDLN-AAKLIHWWQYVALEVDEDLFITARVTASNSSK 300
           ++AWK++LV VP + T F+V K  +++   KL++ WQ +A + D+DL +       N + 
Sbjct: 241 IVAWKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITD 300

Query: 301 EGGR---IMKASFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSAS--EY 360
             G+    +   F S+FLG    LV LM K+FPEL +KK DC ++SW++ + F +    Y
Sbjct: 301 NQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNY 360

Query: 361 VSPE-NLELLLDRTSLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFG 420
            +   N E+LLDR++   G +K+K DY  KPI E+    I E+   E++      L P+G
Sbjct: 361 DTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYG 420

Query: 421 GKMNEISETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAA 480
           G M+EISE+  P PHRA     + Y  +W + + + +HL W R ++++MTP+VS++PR A
Sbjct: 421 GIMDEISESAIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNIYNFMTPYVSKNPRLA 480

Query: 481 YMNYKDLDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPP 531
           Y+NY+DLDIG N+    P +Y +A +WG +YFG NF+RLV+VKT VDP NFFR+EQSIPP
Sbjct: 481 YLNYRDLDIGINDPKN-PNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPP 538

BLAST of CmaCh13G002080 vs. TrEMBL
Match: U5FWX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.4e-164
Identity = 296/527 (56.17%), Postives = 381/527 (72.30%), Query Frame = 1

Query: 6   SNFSFISLLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSI 65
           S   F+ +LL  F SWG  +   EDFL C+SL+S +  + S +I+TP+++SY  VL FSI
Sbjct: 7   SMLPFLLILLFPF-SWGTSAHTHEDFLQCLSLYSEDSAATSKVIYTPNNTSYATVLQFSI 66

Query: 66  RNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFV 125
           RNLRF       PV IVTP   S +QAT+ C + H LQIR RSGGHD+EG SY+S +PFV
Sbjct: 67  RNLRFNSSKLT-PVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVLPFV 126

Query: 126 LIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGG 185
           ++DLINL  IT+D+  +TAW Q+GAT+GELYY I EKSRTLAFPAG   ++G+GG FSGG
Sbjct: 127 ILDLINLRKITVDLSTKTAWVQAGATLGELYYSIAEKSRTLAFPAGACHTVGVGGQFSGG 186

Query: 186 GLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKL 245
           G G L+RKYGLA DNV+DA L+DANG++LDR+SMGEDLFWAIRGGGG SFG+V AWK+ L
Sbjct: 187 GYGGLLRKYGLAADNVIDAELIDANGRVLDRESMGEDLFWAIRGGGGNSFGVVTAWKVNL 246

Query: 246 VRVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKAS 305
           V VPPTVT FAV K    NA KLIH WQYVA ++ ED+ I A V   NSS+ G   ++A+
Sbjct: 247 VEVPPTVTVFAVPKVLKENATKLIHRWQYVANKLPEDIVIAAYVNRINSSQGGNPTIQAT 306

Query: 306 FFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTS 365
           F SLFLG    L+ LM+++FPEL L K+DC+E+SW+E   F A  + S  +L++LLDRT 
Sbjct: 307 FTSLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLAG-FPSNASLDVLLDRTP 366

Query: 366 LITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPH 425
                +K KSDY  +P+ ETAL G+WE F  +++E+  L ++P+GGKM EISE+  P PH
Sbjct: 367 QSITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGGKMEEISESSIPFPH 426

Query: 426 RASHPIHIGYYVTW--HRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN 485
           RA +   I YYV W     +A  RH+ W R L+ YMTP+VS++PR AY+NY+DLD+G NN
Sbjct: 427 RAGNLYKIHYYVAWTEEGKEASERHISWIRRLYSYMTPYVSKNPREAYVNYRDLDLGINN 486

Query: 486 EDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPP 531
             G  TSY +AS+WG +YF  NF++LV +KT+VDP NFFR+EQSIPP
Sbjct: 487 LAG-NTSYKQASIWGRKYFKNNFDKLVRIKTEVDPANFFRNEQSIPP 529

BLAST of CmaCh13G002080 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 8.9e-164
Identity = 292/523 (55.83%), Postives = 380/523 (72.66%), Query Frame = 1

Query: 10  FISLLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLR 69
           F+  LL SF  W   +   EDFL C+SL+S + +S S L++TP++SSY +VL  SI+N R
Sbjct: 13  FLCFLLFSFL-WSASAHTHEDFLQCLSLYSEDSNSFSKLVYTPNNSSYLHVLQGSIQNSR 72

Query: 70  FAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDL 129
           F    TPKP  IVTP   S +QAT+ C + HG+ +R RSGGHD+EG SYVS +PFV+IDL
Sbjct: 73  FNTTATPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVSVLPFVIIDL 132

Query: 130 INLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGM 189
           INL +I +DV++ TAW Q+GAT+GELYY I EKS TLAFPAG   ++G GG  SGGG G 
Sbjct: 133 INLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGGHLSGGGYGG 192

Query: 190 LVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVP 249
           L+RKYGLA DN++DA L+DA G++LDR SMGEDLFWAIRGGGG +FG+V+AWKLKLV VP
Sbjct: 193 LMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWKLKLVPVP 252

Query: 250 PTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSL 309
            TVT F+V +S + NA KLIH WQYVA ++ EDLFITA +T  NSS+EG   ++A F SL
Sbjct: 253 HTVTFFSVLRSLEENATKLIHRWQYVANKLPEDLFITAYITKINSSQEGISTIQAEFPSL 312

Query: 310 FLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITG 369
           FLG A  L+ LM++ FPEL L K+DC E+SWVE + ++ S Y S  +L++LL+RT     
Sbjct: 313 FLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVLYN-SGYSSNSSLDVLLNRTPQYIT 372

Query: 370 RYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASH 429
            +K KSDY  KP+ E A  GIW+RF    +ET +L+L+P+GGKM++ISE+  P  HRA +
Sbjct: 373 NFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISESSIPFAHRAGN 432

Query: 430 PIHIGYYVTWHR--PDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGI 489
              I Y + W+    +A  RH+ W R L+ Y  P+VS++PR AY+NY+DLD+G NN  G 
Sbjct: 433 LYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRDLDVGMNNIQG- 492

Query: 490 PTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPP 531
            TSY +AS+WG +YF  NF+RL++VKT VDP NFFR+EQSIPP
Sbjct: 493 NTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIPP 532

BLAST of CmaCh13G002080 vs. TrEMBL
Match: B9GH04_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46910g PE=4 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 7.5e-163
Identity = 290/526 (55.13%), Postives = 383/526 (72.81%), Query Frame = 1

Query: 6   SNFSFISLLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSI 65
           S   F+  LL+SF SW   +   EDFL C+SLH  +  + S  IHTP +SSY  +L FSI
Sbjct: 7   SMLPFLLCLLISF-SWVISAHPREDFLKCLSLHFEDPAAMSNAIHTPYNSSYSSILQFSI 66

Query: 66  RNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFV 125
           RNLRF      KP+ IVTP  AS +QA ++C + H LQIR RSGGHDFEG SY++ +PFV
Sbjct: 67  RNLRFNSSEL-KPLVIVTPTNASHIQAAILCSQRHNLQIRIRSGGHDFEGLSYMAALPFV 126

Query: 126 LIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGG 185
           +IDLI+L ++ +D    TAW Q+GAT+GELYY I EKSRTLAFPAG   +IG+GG FSGG
Sbjct: 127 IIDLISLRAVNVDATSRTAWVQAGATLGELYYSISEKSRTLAFPAGSCPTIGVGGHFSGG 186

Query: 186 GLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKL 245
           G G +VRK+GLA DNV+DA+L+D+ G++LDR SMGEDLFWAIRGGGG SFG+V+AWK+ L
Sbjct: 187 GHGTMVRKFGLASDNVIDAHLIDSKGRILDRASMGEDLFWAIRGGGGQSFGVVVAWKISL 246

Query: 246 VRVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKAS 305
           V VP TVT F+V ++ + NA KL+H WQYVA  + EDL I  +VT  NSS+EG   ++A+
Sbjct: 247 VEVPSTVTMFSVSRTLEQNATKLLHRWQYVANTLPEDLVIDVQVTRVNSSQEGNTTIQAT 306

Query: 306 FFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTS 365
           FFSLFLG+  +L+ +M+++FPEL L K+DC E+SW+E++ ++   + S  +L++LL+RT 
Sbjct: 307 FFSLFLGEVDQLLPVMQESFPELGLVKDDCFEMSWIESVFYTGG-FTSNASLDVLLNRTP 366

Query: 366 LITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPH 425
               R+K KSDY  +P+ E A  GIWERF  E++E   LILIP+GGKM+EISE+ TP PH
Sbjct: 367 RSIPRFKAKSDYVKEPMPEIAFEGIWERFFEEDIEAPTLILIPYGGKMDEISESSTPFPH 426

Query: 426 RASHPIHIGYYVTWHR--PDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN 485
           RA +   +   V+W     +A  RH+ W R L+ Y+T +VS++PR AY+NY+DLD+G NN
Sbjct: 427 RAGNLYVLVSSVSWREESKEASRRHMAWIRRLYSYLTKYVSKNPREAYVNYRDLDLGINN 486

Query: 486 EDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
             G  TSY +AS+WG +YF  NF+RLV VKT+VDP NFFR+EQSIP
Sbjct: 487 LTG-TTSYKQASIWGRKYFKNNFDRLVRVKTEVDPTNFFRNEQSIP 528

BLAST of CmaCh13G002080 vs. TrEMBL
Match: A0A061G763_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027041 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 1.1e-161
Identity = 284/526 (53.99%), Postives = 388/526 (73.76%), Query Frame = 1

Query: 7   NFSFISLLLLSFA-SWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSI 66
           +FS    LL+ F+ SW   +   + FL C+SLHS     N+ LI+T +SSSY  VL  S+
Sbjct: 5   SFSMPLFLLVLFSFSWATSARTHDSFLQCLSLHSD---INARLIYTQNSSSYESVLELSV 64

Query: 67  RNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFV 126
           RN RF+   TP+P+ IVTP   S +QAT+ C + H +QIR RSGGHD+EG SYVS +PFV
Sbjct: 65  RNARFSTPTTPRPLVIVTPSNVSHIQATINCSREHDMQIRIRSGGHDYEGLSYVSKVPFV 124

Query: 127 LIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGG 186
           +IDLIN+ SI +D +++TAW Q+GAT+GELYY+I EKS TLAFPAG+  ++G+GG FSGG
Sbjct: 125 IIDLINMRSIDVDTEEKTAWIQAGATIGELYYRIAEKSATLAFPAGVCPTVGVGGHFSGG 184

Query: 187 GLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKL 246
           G GM+ RK+GLA D ++DA++V  +G++LDR+SMGEDLFWAIRGGGG SFG+++AWK+KL
Sbjct: 185 GYGMMTRKFGLAADQIIDAHIVTVDGRILDRNSMGEDLFWAIRGGGGASFGVIVAWKVKL 244

Query: 247 VRVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKAS 306
           V VP TVT F V+++ + NA KL+H WQ VA  +DEDL+    + A NSS+EG R ++AS
Sbjct: 245 VSVPSTVTVFTVNRTLEENATKLVHKWQSVAEAIDEDLYFRLFLRAVNSSQEGKRTVQAS 304

Query: 307 FFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTS 366
           F SLFLG+  +L++LM+++FPE  L KEDC+E+SW++++ + A E    E+L++LL+RT 
Sbjct: 305 FTSLFLGRIDQLIALMQESFPEFGLVKEDCIEMSWIQSILYFA-EIPQSESLDVLLNRT- 364

Query: 367 LITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPH 426
           +  G +K KSDY T+PI E AL G+W+ F  +E ET+ LI  P+GG M+EISE++TP PH
Sbjct: 365 VTAGFFKGKSDYVTEPIPEIALEGLWQWFYEDESETLGLIFSPYGGIMDEISESETPFPH 424

Query: 427 RASHPIHIGYYVTWHRPDADS--RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNN 486
           RA +  +I Y V+W   DA +  +H+ + R L+ YM P+VS+SPRAAYMNY+DLD+G NN
Sbjct: 425 RAGNLYNIHYGVSWEEEDASTSQKHINFMRRLYKYMEPYVSKSPRAAYMNYRDLDLGSNN 484

Query: 487 EDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
           + G  TSY +AS W  +YF  NF +L+ VK  VDP NFFRHEQS+P
Sbjct: 485 K-GKYTSYTQASRWALKYFKNNFNKLIHVKNMVDPQNFFRHEQSVP 524

BLAST of CmaCh13G002080 vs. TrEMBL
Match: A0A061GF79_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027038 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 4.1e-161
Identity = 285/523 (54.49%), Postives = 383/523 (73.23%), Query Frame = 1

Query: 13  LLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAE 72
           L+L SF SW   +   + FL C+SLHS     N+ LI+T +SSSY  VL+ S RN RF+ 
Sbjct: 13  LVLFSF-SWATSARTHDSFLQCLSLHSG---INARLIYTQNSSSYESVLDLSARNARFST 72

Query: 73  YHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINL 132
             TP+P+ IVTP   S VQAT+ C + H +QIR RSGGHD+EG SYVS +PFV+IDLIN+
Sbjct: 73  PTTPRPLVIVTPSNVSHVQATINCSREHDMQIRIRSGGHDYEGLSYVSKVPFVIIDLINM 132

Query: 133 NSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVR 192
            SI +D + +TAW Q+GAT+GELYY+I EKS TLAFPAG+  ++G+GG FSGGG GM+ R
Sbjct: 133 RSIDVDTEKKTAWIQAGATIGELYYRIAEKSTTLAFPAGVCPTVGVGGHFSGGGYGMMTR 192

Query: 193 KYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTV 252
           K+GLA D ++DA++V  +G++LDR+SMGEDLFWAIRGGGG SFG+++AWK+KLV VP TV
Sbjct: 193 KFGLAADQIIDAHIVTVDGRILDRNSMGEDLFWAIRGGGGASFGVIVAWKVKLVSVPSTV 252

Query: 253 TSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLG 312
           T F V+++ + NA KL+H WQ VA  +DEDL+    + A NSS+EG R ++ASF SLFLG
Sbjct: 253 TVFTVNRTLEENATKLVHKWQSVAEAIDEDLYFRVFLRAVNSSQEGKRTVQASFTSLFLG 312

Query: 313 KAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLI----T 372
           +  +L++LM+++FPEL L KEDC+E+SW++++ +        E+L++LL+RT  I     
Sbjct: 313 RIDQLIALMQESFPELGLVKEDCIEMSWIQSILYFPG-IPQSESLDVLLNRTGTIRTVTA 372

Query: 373 GRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRAS 432
           G +K KSDY T+PI E AL G+W+ F  +E  T++LI  P+GG M+EISE++TP PHRA 
Sbjct: 373 GIFKAKSDYVTEPIPEIALEGLWQWFYEDESGTLELIFSPYGGIMDEISESETPFPHRAG 432

Query: 433 HPIHIGYYVTWHRPDADS--RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDG 492
           +   I Y V+W   DA +  +H+ + R L+ YM P+VS+SPRAAYMNY+DLD+G NNE G
Sbjct: 433 NLYKIHYAVSWEEEDASTSQKHINFMRRLYKYMEPYVSKSPRAAYMNYRDLDLGSNNE-G 492

Query: 493 IPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
             TSY +AS W  +YF  NF RL+ VK+ VDP NFFRHEQS+P
Sbjct: 493 NYTSYTQASRWALKYFKNNFNRLIHVKSMVDPQNFFRHEQSVP 529

BLAST of CmaCh13G002080 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 490.0 bits (1260), Expect = 2.0e-138
Identity = 250/528 (47.35%), Postives = 351/528 (66.48%), Query Frame = 1

Query: 8   FSFISLLLLSFASWGFGSDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRN 67
           F  ++L L  F S      N E+FL C+S H  N   +S +IHT    SY  +LN SI+N
Sbjct: 14  FLLLNLSLSHFPSISAQRTNHENFLKCLS-HRINE-DDSRIIHTSKDPSYFSILNSSIQN 73

Query: 68  LRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNI-PFVL 127
            RF    TPKPV+I+TP QA+ VQ+T+ C + HG+ IRTRSGGHD+EG SY++   PFV+
Sbjct: 74  PRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVV 133

Query: 128 IDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGG 187
           IDL NL SIT+DVD+ T W QSGAT+GELYY+IG+ S++LAFPAG+  ++G+GG F GGG
Sbjct: 134 IDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGG 193

Query: 188 LGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 247
            G L+RKYGL+ DNV+DA++VDANG  LDR  MGED FWAIRGGGG SF +VL+WK++L+
Sbjct: 194 YGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLL 253

Query: 248 RVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASF 307
            VP  VT F V K+ +  A  +I+ WQY+A +V  DLFI A +            + ASF
Sbjct: 254 DVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQKETE-------VYASF 313

Query: 308 FSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSL 367
             L+LG  ++L++LM+  FPEL L+  +C E+SW+E++ +    ++  E++E+L  R   
Sbjct: 314 PGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FIKGESMEILAKR-KR 373

Query: 368 ITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHR 427
            +  +K K D+  +PI +TA+  +W RF+  E    ++IL PFGGKM+EI++ + P PHR
Sbjct: 374 TSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHR 433

Query: 428 ASHPIHIGYYVTWHRPDADS-----RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGK 487
             +   I Y   W   +  +     ++L W   ++++MTP+VS+SPR AY+N++D+D+G 
Sbjct: 434 EGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGM 493

Query: 488 NNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
                + T Y+EA VWG +YF  NF+RLV VKT VDP +FF  EQSIP
Sbjct: 494 YLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIP 527

BLAST of CmaCh13G002080 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 488.4 bits (1256), Expect = 5.7e-138
Identity = 247/505 (48.91%), Postives = 343/505 (67.92%), Query Frame = 1

Query: 27  NLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQ 86
           N   FL C+SL  ++ +  S +IHTP+ +S+  VL  SI+N RF+    PKPV I+TP Q
Sbjct: 33  NQSSFLQCLSLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQ 92

Query: 87  ASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWA 146
            S VQ+ V C +  G+ IRTRSGGHD+EG SYV++ PFV++DL NL SIT+DVD+ + W 
Sbjct: 93  PSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWV 152

Query: 147 QSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 206
           Q+GAT+GELYY+IG+K+RTLAFPAG+  ++G+GG FSGGG G L+RK+GLA D+V+DA +
Sbjct: 153 QTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARV 212

Query: 207 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 266
           VDA G++L+R  MGED FWAIRGGGG SF +VL+WK+ L+ VP TVT F V K  + +A 
Sbjct: 213 VDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSAL 272

Query: 267 KLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLMEKAFP 326
           K+IH WQ+VA +V +DLFI   +    +      +++ASF  L+LG    L+ ++ K FP
Sbjct: 273 KIIHRWQFVADKVSDDLFIRVMLQRYKN------MVRASFPGLYLGSVKNLLKMVNKEFP 332

Query: 327 ELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKPISETA 386
           EL L+++DC E+SW+E++ + A     P N+     R SL    +K KSD+  +P+ +TA
Sbjct: 333 ELGLEEDDCTEMSWIESVIWFAELGEEPINVLTKRTRASL---AFKAKSDFVQEPMPKTA 392

Query: 387 LNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPDADS 446
           ++ +W R +  E E  QLI  PFGGKM+EI++ +TP PHR  +   I Y   W R D   
Sbjct: 393 ISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYW-RGDVKE 452

Query: 447 RHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTS-YDEASVWGYRYFGIN 506
           +++ W   ++D M+ FV++SPR AY+N +DLD+G     G+  S Y+E   WG +YF  N
Sbjct: 453 KYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGM--YVGVKRSKYEEGKSWGVKYFKNN 512

Query: 507 FERLVEVKTKVDPFNFFRHEQSIPP 531
           FERLV VKT VDP +FF  EQSIPP
Sbjct: 513 FERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of CmaCh13G002080 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 483.0 bits (1242), Expect = 2.4e-136
Identity = 247/511 (48.34%), Postives = 345/511 (67.51%), Query Frame = 1

Query: 27  NLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQ 86
           N EDFL C+S   ++      +IHT   SS+  +L+ SI+N RF+   TPKPV+I+TP +
Sbjct: 27  NHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVK 86

Query: 87  ASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVS-NIPFVLIDLINLNSITIDVDDETAW 146
           AS VQ  + C + HG+ +RTRS GH +EG SY++ N PF +IDL NL SI++DVD+ T W
Sbjct: 87  ASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGW 146

Query: 147 AQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAY 206
            Q+GAT GELYY+IG+ +++LAFPAGI  ++G+GG FSGGG G L+RKYGLA DN++DA 
Sbjct: 147 VQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDAL 206

Query: 207 LVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNA 266
           +VDA+G++LDR +MGED FWAIRGGGG SFG++L+WK+KLV VP T+T F V K+    A
Sbjct: 207 VVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEA 266

Query: 267 AKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLMEKAF 326
            ++I  WQY A +V +DLFI  R T   S+K     + A F  L++G    L++LME+ F
Sbjct: 267 VRIIKKWQYAADKVPDDLFI--RTTLERSNKNA---VHALFTGLYIGPVNNLLALMEEKF 326

Query: 327 PELELKKEDCLEISWVEAMAFSASEYVSPENLELLL--DRTSLITGRYKMKSDYATKPIS 386
           PEL L+KE C E+SW+E++ + A ++   E+L +L   +RTSL    +K K D+  +PI 
Sbjct: 327 PELGLEKEGCEEMSWIESVLWFA-DFPKGESLGVLTNRERTSL---SFKGKDDFVQEPIP 386

Query: 387 ETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPD 446
           E A+  IW R +  E    ++IL PFGGKM+E++E +TP PHR  +   I Y   W   +
Sbjct: 387 EAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWREEE 446

Query: 447 -----ADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWG 506
                   ++L+W   ++++MTP+VS+SPR AY+N+KD+D+G        T Y+E   WG
Sbjct: 447 DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKK-KTKYEEGKSWG 506

Query: 507 YRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
            +YF  NFERLV VKT+VDP +FF  EQSIP
Sbjct: 507 VKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of CmaCh13G002080 vs. TAIR10
Match: AT4G20860.1 (AT4G20860.1 FAD-binding Berberine family protein)

HSP 1 Score: 469.9 bits (1208), Expect = 2.1e-132
Identity = 254/537 (47.30%), Postives = 347/537 (64.62%), Query Frame = 1

Query: 8   FSFISLLLLSFASWGFGSDNLEDFLHCISLHSSNR----------HSNSTLIHTP-SSSS 67
           + F+  L+L   S  + +   E F +C+S    N           H+  + +HT  S SS
Sbjct: 7   YLFLLFLVLCVKSV-YSTPTREQFQNCLSTKQFNSTLKNPINLTTHTLDSRVHTDFSESS 66

Query: 68  YPYVLNFSIRNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGR 127
            P   N S  NL F      KP+ IV P   S+++ +++C +  G+Q+RT SGGHD+EG 
Sbjct: 67  SP---NSSFLNLNFTSL---KPILIVKPKSESEIKQSILCSRKLGVQVRTMSGGHDYEGL 126

Query: 128 SYVSNIPFVLIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASI 187
           SY+S  PF+++DL+NL SI+I++ DETAW QSGAT+GE+YYKI + S+  AF AGI  S+
Sbjct: 127 SYLSLSPFIIVDLVNLRSISINLTDETAWIQSGATLGEVYYKIAKTSKIHAFAAGICPSV 186

Query: 188 GLGGFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFG 247
           G+GG  SGGG G ++RKYGLA DNVVDA L+D NGK LDR +MGEDLFWA+RGGG  SFG
Sbjct: 187 GVGGHISGGGFGTIMRKYGLASDNVVDARLMDVNGKTLDRKTMGEDLFWALRGGGAASFG 246

Query: 248 IVLAWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSK 307
           +VL+WK+KL RVP  VT F        +  KL+H WQ +  E+DEDLFI  RV   NS +
Sbjct: 247 VVLSWKVKLARVPEKVTCFISQHPMGPSMNKLVHRWQSIGSELDEDLFI--RVIIDNSLE 306

Query: 308 EGGRIMKASFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPEN 367
              R +K++F +LFLG    L+ LM + FPEL L+ +DC E+SW+E++ F    + S + 
Sbjct: 307 GNQRKVKSTFQTLFLGGIDRLIPLMNQKFPELGLRSQDCSEMSWIESIMF--FNWRSGQP 366

Query: 368 LELLLDRTSLITGRY-KMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNE 427
           LE+LL+R      +Y K KSDY  KP+ E     + +RF   E +T  +I  P GGK+++
Sbjct: 367 LEILLNRDLRFEDQYFKAKSDYVQKPVPENVFEEVTKRFL--EQDTPLMIFEPLGGKISK 426

Query: 428 ISETDTPNPHRASHPIHIGYYVTWHRPDAD--SRHLEWTRELHDYMTPFVSRSPRAAYMN 487
           ISET++P PHR  +  +I Y V W   + +  ++H+ W R LHDYMTP+VS+SPR AY+N
Sbjct: 427 ISETESPYPHRRGNLYNIQYMVKWKVNEVEEMNKHVRWMRSLHDYMTPYVSKSPRGAYLN 486

Query: 488 YKDLDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPP 531
           Y+DLD+G     GI TS+++A  WG  YF  NF+RL  VK K+DP NFFR+EQSIPP
Sbjct: 487 YRDLDLGSTK--GINTSFEDARKWGETYFKGNFKRLGLVKGKIDPTNFFRNEQSIPP 528

BLAST of CmaCh13G002080 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 466.1 bits (1198), Expect = 3.0e-131
Identity = 235/507 (46.35%), Postives = 328/507 (64.69%), Query Frame = 1

Query: 25  SDNLEDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTP 84
           S N E F  C++ +S  +H  S  I    + SY  VL  +IRNLRF    TPKP  I+  
Sbjct: 22  SANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAA 81

Query: 85  WQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYV--SNIPFVLIDLINLNSITIDVDDE 144
              S VQA + C K H LQ++ RSGGHD++G SYV  S  PF ++D+ NL S+ +DV  +
Sbjct: 82  THESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASK 141

Query: 145 TAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVV 204
           TAW Q+GA +GE+YY I EKS+TLA+PAGI  ++G+GG  SGGG G ++RKYGL VDN +
Sbjct: 142 TAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTI 201

Query: 205 DAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWD 264
           DA +VD NGK+LDR  MGEDL+WAI GGGGGS+G+VLA+K+ LV VP  VT F + ++ +
Sbjct: 202 DARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLE 261

Query: 265 LNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFFSLFLGKAAELVSLME 324
            NA  +IH WQ VA ++ ++LFI   +   N +    + ++ +F ++FLG    L+S++ 
Sbjct: 262 QNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSILN 321

Query: 325 KAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKPI 384
           + FPEL L + DC E SW++++ F  +  V      LLL R   +    K KSDY  +PI
Sbjct: 322 RRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSE-TLLLQRNQPV-NYLKRKSDYVREPI 381

Query: 385 SETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRP 444
           S T L  IW+  K+ ELE   +   P+GG+M  IS T TP P+RA +   I Y   W   
Sbjct: 382 SRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDE 441

Query: 445 DADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRYF 504
               R++E TR+L+ +MTPFVS++PR ++ NY+D+D+G N+ +G  +SY E   +G +YF
Sbjct: 442 TLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYF 501

Query: 505 GINFERLVEVKTKVDPFNFFRHEQSIP 530
             NFERLV++KT+VD  NFFR+EQSIP
Sbjct: 502 AGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of CmaCh13G002080 vs. NCBI nr
Match: gi|659072076|ref|XP_008463307.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 859.4 bits (2219), Expect = 3.5e-246
Identity = 422/541 (78.00%), Postives = 481/541 (88.91%), Query Frame = 1

Query: 1   MKIYWSNF--SFISLLLLSFASWGFGSDNLEDFLHCISLHSSNRH-SNSTLIHTPSSSSY 60
           MK +W  +  SFISLLLLS A+ G GS+N+EDF+ CI +HSSN + SNSTLIHTPSSSSY
Sbjct: 1   MKYFWKCYCYSFISLLLLSLATLGCGSNNMEDFVDCIYVHSSNNNDSNSTLIHTPSSSSY 60

Query: 61  PYVLNFSIRNLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRS 120
            YVLNFSIRNLRF+E  TPKPVAI+TP  ASQVQATVICCK+HGLQIRTRSGGHDFEGRS
Sbjct: 61  SYVLNFSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEGRS 120

Query: 121 YVSNIPFVLIDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIG 180
           YV+N+PFVLIDLINLNSITIDV+DETAW QSGATVGELYYKIGEKSRTL FPAG  A+IG
Sbjct: 121 YVANVPFVLIDLINLNSITIDVEDETAWVQSGATVGELYYKIGEKSRTLGFPAGFKATIG 180

Query: 181 LGGFFSGGGLGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGI 240
           LGGF SGGG+GM+VRKYGL  DNVVDAY+VD NG++++R SMGEDLFWAIRGGGGGSFGI
Sbjct: 181 LGGFLSGGGIGMMVRKYGLGADNVVDAYVVDRNGRVVNRYSMGEDLFWAIRGGGGGSFGI 240

Query: 241 VLAWKLKLVRVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSK- 300
           VLAWKL+LV+VP  VTSFA+HK+WD NAA LI+ WQY+A  VD+DLFI+A VTASNSSK 
Sbjct: 241 VLAWKLRLVQVPSIVTSFALHKTWDQNAANLIYRWQYIAPWVDQDLFISAWVTASNSSKD 300

Query: 301 EGGRIMKASFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPEN 360
           E GRIM+ASFFSLFLG A EL+SLMEK FPEL LKK+DCLE+SWVE+MAFSAS +V  ++
Sbjct: 301 ESGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKKDCLEMSWVESMAFSASGFVYAKS 360

Query: 361 LELLLDRTSLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEI 420
           LELLLDRT +  GRYK KSDYAT+PIS+TAL G+WERFK EELETVQLILIPFGGKMNEI
Sbjct: 361 LELLLDRTPVDNGRYKTKSDYATEPISKTALKGMWERFKDEELETVQLILIPFGGKMNEI 420

Query: 421 SETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKD 480
           SE++TP+PHRA +PIHIGYY+TW +P+ADSR+LEW RELHDYMTPFVS+SPRAAY+NY+D
Sbjct: 421 SESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYVNYRD 480

Query: 481 LDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAPMGG 538
           LD+G NN+DG+PT Y EAS+WG+RYFG NFERL+EVK KVDPFNFFRHEQSIPPA P  G
Sbjct: 481 LDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRVG 540

BLAST of CmaCh13G002080 vs. NCBI nr
Match: gi|449459146|ref|XP_004147307.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 825.5 bits (2131), Expect = 5.6e-236
Identity = 400/512 (78.12%), Postives = 456/512 (89.06%), Query Frame = 1

Query: 28  LEDFLHCISLHSSNRH-SNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQ 87
           ++DF++CI +HSSN + SNSTLIHTPSSSSY YVLNFSIRNLRF+E  TPKPVAI+TP  
Sbjct: 1   MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 88  ASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWA 147
           ASQVQATVICCK+HGLQIRTRSGGHDFEGRSYV+N+PFVLIDLINLNSITIDV+DE+AW 
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 120

Query: 148 QSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYL 207
           QSGATVGELY++IGEKSRTL FPAG  A+IGLGGF SGGG GM+VRKYGL  DNVVDAY+
Sbjct: 121 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 180

Query: 208 VDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAA 267
           VD NG++++R SMGEDLFWAIRGGGGGSFGIVLAWKL+LV+VP  VTSFA+HK WD NAA
Sbjct: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 240

Query: 268 KLIHWWQYVALEVDEDLFITARVTASNSSKEG-GRIMKASFFSLFLGKAAELVSLMEKAF 327
            LI+ WQY+A  VD+DLFI+A VTASNSS +G GRIM+ASFFSLFLG A EL+SLMEK F
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 328 PELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLITGRYKMKSDYATKPISET 387
           PEL LKKEDCLE SWVE+MAFSAS +VS ++LELLLDRT L  GRYK KSDYAT+PISET
Sbjct: 301 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 360

Query: 388 ALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPDAD 447
            L G+WERFK EELETVQLILIPFGGK NEISE++TP+PHRA +PIHIGYY+TW RPDAD
Sbjct: 361 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 420

Query: 448 SRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRYFGIN 507
           S+HL+W RELH+YMTPFVS+SPRAAY+NY+DLD+G NN+DG+PT  +EAS+WG+RYFG N
Sbjct: 421 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 480

Query: 508 FERLVEVKTKVDPFNFFRHEQSIPPAAPMGGI 538
           FERL+EVK KVDPFNFFRHEQSIPPA    GI
Sbjct: 481 FERLMEVKRKVDPFNFFRHEQSIPPAPTSVGI 512

BLAST of CmaCh13G002080 vs. NCBI nr
Match: gi|659068685|ref|XP_008445674.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 602.1 bits (1551), Expect = 1.0e-168
Identity = 300/527 (56.93%), Postives = 391/527 (74.19%), Query Frame = 1

Query: 8   FSFISLLLLSFASWGFGSDNL-EDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIR 67
           FS I +L+ S +SW   S +  ++FL C+S HSS R+  S ++HTP +SSY  VL+FSIR
Sbjct: 10  FSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSYSSVLDFSIR 69

Query: 68  NLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVL 127
           NL F++  TPKP+ I+TP   S +QA VIC K+HGLQIRTRSGGHDFEG SYV+  PF++
Sbjct: 70  NLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFII 129

Query: 128 IDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGG 187
           +DLINL SI IDV+  TAW +SGAT+GELYY+IGEKSRTLAFP G+  ++G+GG FSGGG
Sbjct: 130 VDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGGG 189

Query: 188 LGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 247
            G+++RK+GLA DNV+DAYLVDANGK+ DR SMGEDLFWAIRGGGGGSFGIV+AWK+KLV
Sbjct: 190 YGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKLV 249

Query: 248 RVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASN--SSKEGGRIMKA 307
            VP TVT  + +++   +  KL+H WQYVA ++DE+LF+   +T  N  +++EG     A
Sbjct: 250 SVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPIA 309

Query: 308 SFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRT 367
           +FFSLFLGK   LV+ +   FPEL   KEDC+E SW+E+   +A+   + E+LE LL+RT
Sbjct: 310 TFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNRT 369

Query: 368 SLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNP 427
                  KMKSDY  +PIS   + G+W+R K +++ET QLI IP+GG+M++ISE +TP  
Sbjct: 370 PSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEMETPFS 429

Query: 428 HRASHPIHIGYYVTWHRPD--ADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKN 487
           HRA     IGY+V W      A  +H+ W R+++ YMTPFVS+SPR+AY NY+DLDIG N
Sbjct: 430 HRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDIGAN 489

Query: 488 NEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
            + G  TS  +AS+WG +YFG NF+RLV VKTKVDP++FFRHEQSIP
Sbjct: 490 KKYG-KTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535

BLAST of CmaCh13G002080 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 600.5 bits (1547), Expect = 2.9e-168
Identity = 296/527 (56.17%), Postives = 393/527 (74.57%), Query Frame = 1

Query: 8   FSFISLLLLSFASWGFGSDNLE-DFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIR 67
           FS I +++ S + W   S +   +FL C+S HSS  +  S ++HTP +SSY  VLNFSIR
Sbjct: 10  FSLILIVVSSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSSYSSVLNFSIR 69

Query: 68  NLRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVL 127
           NLRF++  TPKP+ I+TP   S ++A VIC K+HGLQIRTRSGGHD+EG SYV++ PF++
Sbjct: 70  NLRFSKPETPKPLLIITPSHVSHIRAAVICSKSHGLQIRTRSGGHDYEGLSYVASHPFII 129

Query: 128 IDLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGG 187
           +DLINL S+ IDV+  TAW +SG T+GELYY+IGEKSRTLAFPAGI  ++G+GG FSGGG
Sbjct: 130 LDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGG 189

Query: 188 LGMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLV 247
            G+++RK+GLA DNV+DAYLVDANGK+ DR+SMGEDLFWAIRGGGGGSFGIV+AWK+KLV
Sbjct: 190 YGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLV 249

Query: 248 RVPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASN--SSKEGGRIMKA 307
            VP TVT    +++   +  KL+H WQYVA ++DE+LF+   +T  N  +++EG     A
Sbjct: 250 SVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITTTQEGITNPIA 309

Query: 308 SFFSLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRT 367
           +FFSLFLGK  ELV+ +   FPEL L K+DC+E SW+E+   +++   + E+LE LL+RT
Sbjct: 310 TFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVESLEPLLNRT 369

Query: 368 SLITGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNP 427
                  K+KSDY  +PIS   + GIW+R K +++ET QLI IP+GG+M++ISE++TP  
Sbjct: 370 PSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQISESETPFS 429

Query: 428 HRASHPIHIGYYVTWHRPD--ADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKN 487
           HR  +   IGY + W      A  +H+ W RE+++YMTPFVS+SPR+AY NY+DLDIG N
Sbjct: 430 HRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYANYRDLDIGVN 489

Query: 488 NEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIP 530
            + G  TS  +AS+WG +YFG NF+RLV VKTKVDP++FFRHEQSIP
Sbjct: 490 KKYG-KTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535

BLAST of CmaCh13G002080 vs. NCBI nr
Match: gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 600.1 bits (1546), Expect = 3.8e-168
Identity = 292/505 (57.82%), Postives = 382/505 (75.64%), Query Frame = 1

Query: 29  EDFLHCISLHSSNRHSNSTLIHTPSSSSYPYVLNFSIRNLRFAEYHTPKPVAIVTPWQAS 88
           ++FLHC+  HSS  +  S +++TP +SSY  VLNFSIRN RF++  TPKP+ I+TP   S
Sbjct: 34  QNFLHCLPEHSSKSYPISKVVYTPINSSYSSVLNFSIRNFRFSKPETPKPLLIITPSHVS 93

Query: 89  QVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLIDLINLNSITIDVDDETAWAQS 148
            +QA VIC K+HGLQIRTRSGGHD+EG SYV+  PF+++DLIN+ SI++D+ + TAW +S
Sbjct: 94  HIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVDLINIRSISVDIVNNTAWVES 153

Query: 149 GATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGLGMLVRKYGLAVDNVVDAYLVD 208
           GAT+GELYY+IGEKSRTLAFPAGI  ++G+GG FSGGG G L+RKYGLA DNV+DAYLVD
Sbjct: 154 GATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLVD 213

Query: 209 ANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVRVPPTVTSFAVHKSWDLNAAKL 268
           ANG   DR+SMGEDLFWAIRGGGGGSFGIV+AWK+KLV VP  VT  +  K+ + +A K+
Sbjct: 214 ANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPALVTICSTIKNLEGDAVKV 273

Query: 269 IHWWQYVALEVDEDLFITARVTASNSSKEGG-RIMKASFFSLFLGKAAELVSLMEKAFPE 328
           IH WQYVA ++ ED+F+   +T  N+S +GG +   A+F+SLFLGK  ELV+ +   FPE
Sbjct: 274 IHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKKPTATFYSLFLGKVDELVATLSTTFPE 333

Query: 329 LELKKEDCLEISWVEAMAFSAS-EYVSPENLELLLDRTSLITGRYKMKSDYATKPISETA 388
             L K+DCLE+ W+E+    AS  + + E+L+ LL+RT       K+KSDY  KPI + A
Sbjct: 334 FGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRTPSTIDSTKIKSDYIKKPIPKAA 393

Query: 389 LNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRASHPIHIGYYVTWHRPD--A 448
           + GIW+R K  ++E  QL+ +P+G +M++ISE++TP  HRA +   IGY V W   +  A
Sbjct: 394 IEGIWQRLKAGDIEAPQLVFVPYGARMSQISESETPFSHRAGYLYKIGYMVGWKGQNLKA 453

Query: 449 DSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGIPTSYDEASVWGYRYFGI 508
            +RH+ WTREL++YMTPFVS+SPR AY+NY+DLDIG NN+ G  TSY +AS+WG +YFG 
Sbjct: 454 KNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSNNKYG-KTSYKQASIWGLKYFGN 513

Query: 509 NFERLVEVKTKVDPFNFFRHEQSIP 530
           NF+RLV VK KVDP +FFRHEQSIP
Sbjct: 514 NFKRLVYVKNKVDPHDFFRHEQSIP 537