Lsi03G001460 (gene) Bottle gourd (USVL1VR-Ls)

BLAST of Lsi03G001460 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 9.1e-141
Identity = 247/514 (48.05%), Postives = 349/514 (67.90%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           E+F+ C   H  NN  +N  L++T     Y  +LN +I+NLRF    TPKPL I+TPS+ 
Sbjct: 32  ENFLKCFSKHIPNNV-ANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QAT++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N++SI ID   ++AWV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY I EK++ L FP G+ PT+GVGG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271

Query: 273 KLIHGWQYIAPEVDQDL-FISAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL + WQ IA + D+DL  ++ ++T + + N G+    +   F S+F G    L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC E SW++   F  SG V+  +     E+LLDR+  K     IK DY  
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI ETA+  + E+   E++     +L P+GG M EIS++  P P+RAG    + Y  +W
Sbjct: 392 KPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N    P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPP 539
           +YFG NF RL++VK KVDP NFFR+EQSIPP PP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of Lsi03G001460 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.5e-138
Identity = 241/514 (46.89%), Postives = 345/514 (67.12%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           E+F+ C +     NN +N   I+T     Y  VLN +I+NLRF+   TPKPL I+TPS+ 
Sbjct: 32  ENFLKC-FSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QA+++C K  GLQ+RTRSGGHD EG SY++ VPF ++DL N++++ +D   ++AWV+
Sbjct: 92  SHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY I E ++   FP G+ PT+GVGG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLV 271

Query: 273 KLIHGWQYIAPEVDQDLFISAWVTASN-SSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL + WQ IA + D+DL ++      N + N G+    +   F S+FLG    L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC E+SW++   F  SG V+  +     E+LLDR+  K     IK DY  
Sbjct: 332 SFPELGIKKTDCKELSWIDTTIF-YSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           + I ETA+  + E+   EE+     +L P+GG M+EIS++  P P+RAG    + Y  TW
Sbjct: 392 KLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N +  P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPES-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPP 539
           +YFG NF RL++VK K DP NFFR+EQSIPP PP
Sbjct: 512 KYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of Lsi03G001460 vs. Swiss-Prot
Match: CBDAS_CANSA (Cannabidiolic acid synthase OS=Cannabis sativa GN=CBDAS PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 7.2e-138
Identity = 242/513 (47.17%), Postives = 352/513 (68.62%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           E+F+ C   +  NN  +N  L++T ++  Y  VLN +I NLRF+   TPKPL I+TPSH 
Sbjct: 32  ENFLKCFSQYIPNNA-TNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +Q T++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N+ SI ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY + EK++ L   AG+ PT+  GG   GGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWA+RGGG  SFGI++AWK++LV VP   T F++ +  +     
Sbjct: 212 NVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK-STMFSVKKIMEIHELV 271

Query: 273 KLIHGWQYIAPEVDQDLFI-SAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL++ WQ IA + D+DL + + ++T + + N G+    +   F S+FLG    L+ LM K
Sbjct: 272 KLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTE----SLELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC ++SW++ + F  SG V+ +    + E+LLDR+  +NG  KIK DY  
Sbjct: 332 SFPELGIKKTDCRQLSWIDTIIF-YSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI E+    + E+   E++      L P+GG M+EIS++  P P+RAG    + Y  +W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KHL W R ++++MTP+VSK+PR+AY+NYRDLDIG N+    P +Y +A  WG 
Sbjct: 452 EKQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKN-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 538
           +YFG NF+RL++VK  VDP NFFR+EQSIPP P
Sbjct: 512 KYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLP 540

BLAST of Lsi03G001460 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.7e-137
Identity = 237/515 (46.02%), Postives = 352/515 (68.35%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           E+F+ C   +   N  +N+ L++T     Y  +LN +I+NLRF+   TPKPL IITP + 
Sbjct: 32  ENFLKCFSQYIPTNV-TNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +Q T++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N++S+ ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY I E ++ L FPAG+ PT+G GG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK++LV VP + T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271

Query: 273 KLIHGWQYIAPEVDQDLFI-SAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL++ WQ IA   +++L + + ++T + + N G+    + + F S+F G    L+ LM K
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC ++SW++ + F  SG V+  +     E+LLDR+  +     IK DY  
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIF-YSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI ETA+  + E+   E++     +  P+GG M+EIS++  P P+RAG    I Y  +W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N +  P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 540
           +YFG NF RL++VK KVDP NFFR+EQSIPP P R
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLR 543

BLAST of Lsi03G001460 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.7e-137
Identity = 236/515 (45.83%), Postives = 352/515 (68.35%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           E+F+ C+  +   N  +N+ L++T     Y  +LN +++NLRF+   TPKPL I TP + 
Sbjct: 32  ENFLKCLSQYIPTNV-TNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +Q T++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N++S+ ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           SGAT+GE+YY I E ++ L FPAG+ PT+G GG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 SGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK++LV VP + T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELV 271

Query: 273 KLIHGWQYIAPEVDQDLFI-SAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL++ WQ IA   +++L + + ++T + + N G+    + + F S+F G    L+ LM K
Sbjct: 272 KLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC ++SW++ + F  SG V+  +     E+LLDR+  +     IK DY  
Sbjct: 332 SFPELGIKKTDCKQLSWIDTIIF-YSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI ETA+  + E+   E++     +  P+GG M+EIS++  P P+RAG    I Y  +W
Sbjct: 392 KPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N +  P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFES-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 540
           +YFG NF RL++VK KVDP NFFR+EQSIPP P R
Sbjct: 512 KYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLPLR 543

BLAST of Lsi03G001460 vs. TrEMBL
Match: A0A061G763_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027041 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.7e-165
Identity = 282/504 (55.95%), Postives = 380/504 (75.40%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           + F+ C+ LHS    D N+ LI+T +SSSY  VL  S+RN RFS P TP+PL I+TPS+ 
Sbjct: 28  DSFLQCLSLHS----DINARLIYTQNSSSYESVLELSVRNARFSTPTTPRPLVIVTPSNV 87

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QAT+ C + H +Q+R RSGGHD+EG SYV+ VPFV+IDL+N+ SI +DTE+++AW+Q
Sbjct: 88  SHIQATINCSREHDMQIRIRSGGHDYEGLSYVSKVPFVIIDLINMRSIDVDTEEKTAWIQ 147

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GELYYRI EKS TL FPAG  PT+GVGG  SGGG GMM RK+GLAAD ++DA++V
Sbjct: 148 AGATIGELYYRIAEKSATLAFPAGVCPTVGVGGHFSGGGYGMMTRKFGLAADQIIDAHIV 207

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAK 272
             +GR+++R SMGEDLFWAIRGGGG SFG+++AWK+KLV VP  VT F ++RT ++NA K
Sbjct: 208 TVDGRILDRNSMGEDLFWAIRGGGGASFGVIVAWKVKLVSVPSTVTVFTVNRTLEENATK 267

Query: 273 LIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFISLFLGKATELLALMEKTFPE 332
           L+H WQ +A  +D+DL+   ++ A NSS  G+R ++ASF SLFLG+  +L+ALM+++FPE
Sbjct: 268 LVHKWQSVAEAIDEDLYFRLFLRAVNSSQEGKRTVQASFTSLFLGRIDQLIALMQESFPE 327

Query: 333 LGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEPISETAL 392
            GL KEDC+EMSW++++ + A     +ESL++LL+RT    G  K KSDY  EPI E AL
Sbjct: 328 FGLVKEDCIEMSWIQSILYFAE-IPQSESLDVLLNRTVTA-GFFKGKSDYVTEPIPEIAL 387

Query: 393 EGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQRPEADS- 452
           EG+W+ F  +E ET+ LI  P+GG M+EIS++ETP P+RAG   +I Y V+W+  +A + 
Sbjct: 388 EGLWQWFYEDESETLGLIFSPYGGIMDEISESETPFPHRAGNLYNIHYGVSWEEEDASTS 447

Query: 453 -KHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNN 512
            KH+ + R L+ YM P+VSKSPR AY+NYRDLD+G+NN+ G   SY +AS W  +YF NN
Sbjct: 448 QKHINFMRRLYKYMEPYVSKSPRAAYMNYRDLDLGSNNK-GKYTSYTQASRWALKYFKNN 507

Query: 513 FERLMEVKMKVDPFNFFRHEQSIP 535
           F +L+ VK  VDP NFFRHEQS+P
Sbjct: 508 FNKLIHVKNMVDPQNFFRHEQSVP 524

BLAST of Lsi03G001460 vs. TrEMBL
Match: A0A061GF79_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027038 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 3.7e-165
Identity = 284/508 (55.91%), Postives = 378/508 (74.41%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           + F+ C+ LHS      N+ LI+T +SSSY  VL+ S RN RFS P TP+PL I+TPS+ 
Sbjct: 28  DSFLQCLSLHSG----INARLIYTQNSSSYESVLDLSARNARFSTPTTPRPLVIVTPSNV 87

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S VQAT+ C + H +Q+R RSGGHD+EG SYV+ VPFV+IDL+N+ SI +DTE ++AW+Q
Sbjct: 88  SHVQATINCSREHDMQIRIRSGGHDYEGLSYVSKVPFVIIDLINMRSIDVDTEKKTAWIQ 147

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GELYYRI EKS TL FPAG  PT+GVGG  SGGG GMM RK+GLAAD ++DA++V
Sbjct: 148 AGATIGELYYRIAEKSTTLAFPAGVCPTVGVGGHFSGGGYGMMTRKFGLAADQIIDAHIV 207

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAK 272
             +GR+++R SMGEDLFWAIRGGGG SFG+++AWK+KLV VP  VT F ++RT ++NA K
Sbjct: 208 TVDGRILDRNSMGEDLFWAIRGGGGASFGVIVAWKVKLVSVPSTVTVFTVNRTLEENATK 267

Query: 273 LIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFISLFLGKATELLALMEKTFPE 332
           L+H WQ +A  +D+DL+   ++ A NSS  G+R ++ASF SLFLG+  +L+ALM+++FPE
Sbjct: 268 LVHKWQSVAEAIDEDLYFRVFLRAVNSSQEGKRTVQASFTSLFLGRIDQLIALMQESFPE 327

Query: 333 LGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRT----PPKNGRHKIKSDYAIEPIS 392
           LGL KEDC+EMSW++++ +   G   +ESL++LL+RT        G  K KSDY  EPI 
Sbjct: 328 LGLVKEDCIEMSWIQSILY-FPGIPQSESLDVLLNRTGTIRTVTAGIFKAKSDYVTEPIP 387

Query: 393 ETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQRPE 452
           E ALEG+W+ F  +E  T++LI  P+GG M+EIS++ETP P+RAG    I Y V+W+  +
Sbjct: 388 EIALEGLWQWFYEDESGTLELIFSPYGGIMDEISESETPFPHRAGNLYKIHYAVSWEEED 447

Query: 453 ADS--KHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRY 512
           A +  KH+ + R L+ YM P+VSKSPR AY+NYRDLD+G+NNE G   SY +AS W  +Y
Sbjct: 448 ASTSQKHINFMRRLYKYMEPYVSKSPRAAYMNYRDLDLGSNNE-GNYTSYTQASRWALKY 507

Query: 513 FGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           F NNF RL+ VK  VDP NFFRHEQS+P
Sbjct: 508 FKNNFNRLIHVKSMVDPQNFFRHEQSVP 529

BLAST of Lsi03G001460 vs. TrEMBL
Match: U5FWX5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16570g PE=4 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 4.8e-165
Identity = 289/525 (55.05%), Postives = 386/525 (73.52%), Query Frame = 1

Query: 13  FISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRN 72
           F+ +LLFP   + G      EDF+ C+ L+S ++  + S +I+TP+++SY+ VL FSIRN
Sbjct: 11  FLLILLFPF--SWGTSAHTHEDFLQCLSLYSEDSA-ATSKVIYTPNNTSYATVLQFSIRN 70

Query: 73  LRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLI 132
           LRF+  +   P+ I+TP+  S +QAT+ C + H LQ+R RSGGHD+EG SY++ +PFV++
Sbjct: 71  LRFNSSKL-TPVVIVTPTILSHIQATIHCSQKHNLQIRIRSGGHDYEGLSYMSVLPFVIL 130

Query: 133 DLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGL 192
           DL+NL  IT+D   ++AWVQ+GAT+GELYY I EKS+TL FPAG   T+GVGG  SGGG 
Sbjct: 131 DLINLRKITVDLSTKTAWVQAGATLGELYYSIAEKSRTLAFPAGACHTVGVGGQFSGGGY 190

Query: 193 GMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 252
           G ++RKYGLAADNV+DA ++DANGRV++R SMGEDLFWAIRGGGG SFG+V AWK+ LV 
Sbjct: 191 GGLLRKYGLAADNVIDAELIDANGRVLDRESMGEDLFWAIRGGGGNSFGVVTAWKVNLVE 250

Query: 253 VPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFI 312
           VPP VT FA+ +   +NA KLIH WQY+A ++ +D+ I+A+V   NSS GG   ++A+F 
Sbjct: 251 VPPTVTVFAVPKVLKENATKLIHRWQYVANKLPEDIVIAAYVNRINSSQGGNPTIQATFT 310

Query: 313 SLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPK 372
           SLFLG    LL LM+++FPELGL K+DC+E+SW+E   F A GF S  SL++LLDRTP  
Sbjct: 311 SLFLGGVDRLLPLMQESFPELGLVKDDCIELSWIEFALFLA-GFPSNASLDVLLDRTPQS 370

Query: 373 NGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRA 432
               K KSDY  +P+ ETALEGMWE F  +++E+  L ++P+GGKM EIS++  P P+RA
Sbjct: 371 ITSFKAKSDYVKQPLPETALEGMWETFFEKDIESPSLFMVPYGGKMEEISESSIPFPHRA 430

Query: 433 GYPIHIGYYVTW--QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNED 492
           G    I YYV W  +  EA  +H+ W R L+ YMTP+VSK+PR AYVNYRDLD+G NN  
Sbjct: 431 GNLYKIHYYVAWTEEGKEASERHISWIRRLYSYMTPYVSKNPREAYVNYRDLDLGINNLA 490

Query: 493 GIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPP 536
           G   SY++AS WG +YF NNF++L+ +K +VDP NFFR+EQSIPP
Sbjct: 491 G-NTSYKQASIWGRKYFKNNFDKLVRIKTEVDPANFFRNEQSIPP 529

BLAST of Lsi03G001460 vs. TrEMBL
Match: A0A061G8F0_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027036 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.4e-164
Identity = 291/526 (55.32%), Postives = 390/526 (74.14%), Query Frame = 1

Query: 13  FISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRN 72
           F+ LL   S AT     D+   F+ C+ LHS    D N+ LI+T +SSSYS VL  S+RN
Sbjct: 11  FLLLLFSFSWATFARTHDS---FLQCLSLHS----DINARLIYTQNSSSYSSVLELSVRN 70

Query: 73  LRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLI 132
            RFS P TP+PL I+TPS+ S +QAT+ C + H +Q+R RSGGHD+EG SYV+ VPFV+I
Sbjct: 71  ARFSTPTTPRPLVIVTPSNVSHIQATINCSREHDMQIRIRSGGHDYEGLSYVSKVPFVII 130

Query: 133 DLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGL 192
           DL+N+ SI +DTE+ +AW+Q+GAT+GELYYRI EKS+TL FPAG  PT+GVGG  SGGG 
Sbjct: 131 DLINMRSIDVDTENSTAWIQAGATIGELYYRIAEKSETLAFPAGVCPTVGVGGHFSGGGY 190

Query: 193 GMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 252
           GMM+RK+GLAAD ++DA++VD +GR+++R SMGEDLFWAIRGGGG SFG+++AWK+KLV 
Sbjct: 191 GMMMRKFGLAADQIIDAHLVDVHGRILDRNSMGEDLFWAIRGGGGASFGVIVAWKVKLVS 250

Query: 253 VPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFI 312
           VP  VT F  +RT ++NA KL+H WQ +A ++ +DL+I  ++ A NSS  G R ++ASF 
Sbjct: 251 VPSTVTVFIANRTLEENATKLVHKWQSVAEKIHEDLYIRVFLRAVNSSQEGNRTIQASFT 310

Query: 313 SLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPK 372
           SLFLG+  +L+ LM+++FPELGL KEDC+EMSW++++ + A G   +ESL++LL+RT   
Sbjct: 311 SLFLGRIDQLIPLMQESFPELGLVKEDCIEMSWIQSILYFA-GIPQSESLDVLLNRTDTA 370

Query: 373 NGRHKIKSDYAIEPISETALEGMWERFKIEELETV--QLILIPFGGKMNEISKAETPSPY 432
           +   K KSDY  EPI E ALEG+W+ F  E+LE V  +++  P+GG MNEI ++ETP P+
Sbjct: 371 S-FFKGKSDYVTEPIPEIALEGLWQWF-YEDLEIVWFEILFSPYGGIMNEIPESETPFPH 430

Query: 433 RAGYPIHIGYYVTWQRPEADS--KHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNN 492
           R+G   +I Y V W   +A +  K++ + R L+ YM P+VSKSPR AY+NYRDLD+G+NN
Sbjct: 431 RSGNLFNIHYVVVWGEEDASTAQKNINFMRRLYKYMEPYVSKSPRAAYMNYRDLDLGSNN 490

Query: 493 EDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           + G   SY +AS WG +YF NNF RL+ VK  VDP NFFRHEQSIP
Sbjct: 491 K-GNYTSYRQASRWGLKYFKNNFNRLIRVKKMVDPQNFFRHEQSIP 525

BLAST of Lsi03G001460 vs. TrEMBL
Match: B9N5Q8_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16580g PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 4.1e-164
Identity = 287/525 (54.67%), Postives = 385/525 (73.33%), Query Frame = 1

Query: 13  FISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRN 72
           F+  LLF  L +        EDF+ C+ L+S ++N S S L++TP++SSY +VL  SI+N
Sbjct: 13  FLCFLLFSFLWSAS--AHTHEDFLQCLSLYSEDSN-SFSKLVYTPNNSSYLHVLQGSIQN 72

Query: 73  LRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLI 132
            RF+   TPKP  I+TP + S +QAT+ C + HG+ +R RSGGHD+EG SYV+ +PFV+I
Sbjct: 73  SRFNTTATPKPPVIVTPMNVSHIQATIFCSQKHGMNVRIRSGGHDYEGLSYVSVLPFVII 132

Query: 133 DLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGL 192
           DL+NL +I +D E+ +AWVQ+GAT+GELYY I EKS TL FPAG  PT+G GG LSGGG 
Sbjct: 133 DLINLRAINVDVENSTAWVQAGATLGELYYSIAEKSGTLAFPAGACPTVGAGGHLSGGGY 192

Query: 193 GMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 252
           G ++RKYGLAADN++DA ++DA GR+++R SMGEDLFWAIRGGGG +FG+V+AWKLKLVP
Sbjct: 193 GGLMRKYGLAADNIIDAQLIDAKGRILDRASMGEDLFWAIRGGGGNTFGVVVAWKLKLVP 252

Query: 253 VPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFI 312
           VP  VT F++ R+ ++NA KLIH WQY+A ++ +DLFI+A++T  NSS  G   ++A F 
Sbjct: 253 VPHTVTFFSVLRSLEENATKLIHRWQYVANKLPEDLFITAYITKINSSQEGISTIQAEFP 312

Query: 313 SLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPK 372
           SLFLG A  LL LM++ FPELGL K+DC EMSWVE + ++ SG+ S  SL++LL+RTP  
Sbjct: 313 SLFLGGADRLLPLMQENFPELGLVKDDCTEMSWVEFVLYN-SGYSSNSSLDVLLNRTPQY 372

Query: 373 NGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRA 432
               K KSDY  +P+ E A EG+W+RF    +ET +L+L+P+GGKM++IS++  P  +RA
Sbjct: 373 ITNFKAKSDYVKKPMPEIAFEGIWKRFLKVGIETPRLVLVPYGGKMDQISESSIPFAHRA 432

Query: 433 GYPIHIGYYVTW--QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNED 492
           G    I Y + W  Q  EA  +H+ W R L+ Y  P+VSK+PR AY+NYRDLD+G NN  
Sbjct: 433 GNLYKIQYLLLWNEQGKEASMRHIAWIRRLYSYTAPYVSKNPREAYINYRDLDVGMNNIQ 492

Query: 493 GIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPP 536
           G   SY++AS WG +YF NNF+RL++VK  VDP NFFR+EQSIPP
Sbjct: 493 G-NTSYKQASIWGRKYFKNNFDRLIQVKTAVDPDNFFRNEQSIPP 532

BLAST of Lsi03G001460 vs. TAIR10
Match: AT5G44410.1 (AT5G44410.1 FAD-binding Berberine family protein)

HSP 1 Score: 495.0 bits (1273), Expect = 6.3e-140
Identity = 257/542 (47.42%), Postives = 356/542 (65.68%), Query Frame = 1

Query: 1   MEYFWSYYYYYCFISLLL--FPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPS 60
           + +  S + Y+  ++L L  FPS++       N E+F+ C+   S   N+ +S +IHT  
Sbjct: 4   LRFLLSLFIYFLLLNLSLSHFPSISAQR---TNHENFLKCL---SHRINEDDSRIIHTSK 63

Query: 61  SSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDF 120
             SY  +LN SI+N RF   ETPKP++IITP   + VQ+T+ C + HG+ +RTRSGGHD+
Sbjct: 64  DPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDY 123

Query: 121 EGRSYVANV-PFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGF 180
           EG SY+A   PFV+IDL NL SIT+D ++ + WVQSGAT+GELYY IG+ S++L FPAG 
Sbjct: 124 EGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGL 183

Query: 181 APTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGG 240
            PT+G+GG   GGG G ++RKYGL+ADNV+DA++VDANG  ++R  MGED FWAIRGGGG
Sbjct: 184 YPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGG 243

Query: 241 GSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTAS 300
            SF +VL+WK++L+ VP +VT F + +T ++ A  +I+ WQYIA +V  DLFI A +   
Sbjct: 244 SSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQK- 303

Query: 301 NSSNGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFV 360
                 E  + ASF  L+LG  ++LLALM+  FPELGL+  +C EMSW+E++ +    F+
Sbjct: 304 ------ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FI 363

Query: 361 STESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGK 420
             ES+E+L  R        K K D+  EPI +TA++ +W RF+  E    ++IL PFGGK
Sbjct: 364 KGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGK 423

Query: 421 MNEISKAETPSPYRAGYPIHIGYYVTWQRPEADS-----KHLEWTRELHDYMTPFVSKSP 480
           M+EI+  E P P+R G    I Y   W   E  +     K+L W   ++++MTP+VSKSP
Sbjct: 424 MSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSP 483

Query: 481 RVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQS 535
           R AYVN+RD+D+G      +   YEEA  WG +YF NNF+RL+ VK  VDP +FF  EQS
Sbjct: 484 RRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQS 527

BLAST of Lsi03G001460 vs. TAIR10
Match: AT4G20820.1 (AT4G20820.1 FAD-binding Berberine family protein)

HSP 1 Score: 493.4 bits (1269), Expect = 1.8e-139
Identity = 259/534 (48.50%), Postives = 349/534 (65.36%), Query Frame = 1

Query: 13  FISLLLFPSLATL--------GYGGDNLEDFVNCIYLHSSNNNDSN--STLIHTPSSSSY 72
           F S+L+F + +TL             N   F+ C+ L     NDSN  S +IHTP+ +S+
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL---NDSNIVSKVIHTPNDTSF 66

Query: 73  SYVLNFSIRNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRS 132
           S VL  SI+N RFS P+ PKP+ I+TP   S VQ+ V C +  G+ +RTRSGGHD+EG S
Sbjct: 67  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 126

Query: 133 YVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIG 192
           YV + PFV++DL NL SIT+D ++ S WVQ+GAT+GELYY IG+K++TL FPAG  PT+G
Sbjct: 127 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 186

Query: 193 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGI 252
           VGG  SGGG G ++RK+GLAAD+V+DA VVDA GR++ R  MGED FWAIRGGGG SF +
Sbjct: 187 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 246

Query: 253 VLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNG 312
           VL+WK+ L+ VP  VT F + +  +Q+A K+IH WQ++A +V  DLFI   +        
Sbjct: 247 VLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRY----- 306

Query: 313 GERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL 372
            + ++ ASF  L+LG    LL ++ K FPELGL+++DC EMSW+E++ + A   +  E +
Sbjct: 307 -KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPI 366

Query: 373 ELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEIS 432
            +L  RT       K KSD+  EP+ +TA+  +W R +  E E  QLI  PFGGKM+EI+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 433 KAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDL 492
             ETP P+R G    I Y   W R +   K++ W   ++D M+ FV+KSPR AY+N RDL
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 486

Query: 493 DIGTNNEDGIPIS-YEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPP 536
           D+G     G+  S YEE  +WG +YF NNFERL+ VK  VDP +FF  EQSIPP
Sbjct: 487 DLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of Lsi03G001460 vs. TAIR10
Match: AT5G44440.1 (AT5G44440.1 FAD-binding Berberine family protein)

HSP 1 Score: 485.0 bits (1247), Expect = 6.5e-137
Identity = 254/537 (47.30%), Postives = 350/537 (65.18%), Query Frame = 1

Query: 4   FWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYS 63
           F S+ +     SL + P ++     G N EDF+ C+  +  N+N     +IHT   SS+ 
Sbjct: 3   FSSFLFTILLFSLNISPLVSA---HGSNHEDFLKCLS-YRMNDNTVEPKVIHTSKDSSFF 62

Query: 64  YVLNFSIRNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSY 123
            +L+ SI+N RFS  ETPKP++IITP   S VQ  + C + HG+ +RTRS GH +EG SY
Sbjct: 63  SILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSY 122

Query: 124 VA-NVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIG 183
           +A N PF +IDL NL SI++D ++ + WVQ+GAT GELYY IG+ +++L FPAG  PT+G
Sbjct: 123 IAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVG 182

Query: 184 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGI 243
           VGG  SGGG G ++RKYGLAADN++DA VVDA+GR+++R +MGED FWAIRGGGG SFG+
Sbjct: 183 VGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGV 242

Query: 244 VLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNG 303
           +L+WK+KLV VP  +T F + +T  + A ++I  WQY A +V  DLFI   +  SN    
Sbjct: 243 ILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN---- 302

Query: 304 GERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL 363
            +  + A F  L++G    LLALME+ FPELGL+KE C EMSW+E++ + A  F   ESL
Sbjct: 303 -KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESL 362

Query: 364 ELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEIS 423
            +L +R        K K D+  EPI E A++ +W R +  E    ++IL PFGGKM+E++
Sbjct: 363 GVLTNRERTSLS-FKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMA 422

Query: 424 KAETPSPYRAGYPIHIGYYVTWQRPE-----ADSKHLEWTRELHDYMTPFVSKSPRVAYV 483
           + ETP P+R G    I Y   W+  E        K+L+W   ++++MTP+VSKSPR AYV
Sbjct: 423 EYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYV 482

Query: 484 NYRDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           N++D+D+G          YEE  +WG +YF NNFERL+ VK +VDP +FF  EQSIP
Sbjct: 483 NFKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Lsi03G001460 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 1.1e-136
Identity = 252/528 (47.73%), Postives = 347/528 (65.72%), Query Frame = 1

Query: 13  FISLLLFPSLATLGYGGDNLE-DFVNCIYLHSSNNNDSNSTLI-HTPSSSSYSYVLNFSI 72
           F+  LL  SL+ +      ++ DFV C+  +S  +   +S+   H  ++SS+   L  S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVP-- 132
           +NLR+  P  PKP  I  P + + VQA V+C K   L LR RSGGHD+EG SYV+ +   
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 133 FVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLS 192
           FV++DL  L  I++D E  SAWV +GA++GE+YYRI EKS+  GFPAG   ++G+GG + 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 193 GGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKL 252
           GG  G M+RK+GL ADNV+DA +VDA+G+++NR +MGED+FWAIRGGGGGSFG++LAWK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 253 KLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWV-TASNSSNGGERIM 312
           KLVPVP IVT F + RT +Q+  KL++ WQ +A ++D+DLFI   +   S +    ER +
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 313 EASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLD 372
             S+   FLG A  LL +M+++FP+LGL K+DCLE SW++++ + A GF ST   E LLD
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEALLD 371

Query: 373 RTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETP 432
                    K KSDY  EPI    LEG+WE  K+ E ++   I  P+GG M +I + ETP
Sbjct: 372 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWE--KLLEEDSPLTIWNPYGGMMAKIPETETP 431

Query: 433 SPYRAGYPIHIGYYVTWQRPE-ADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGT 492
            P+R+G    I +   WQ  + +++KH+ W RE++ YM  +VSKSPR AYVNYRDLD+G 
Sbjct: 432 FPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGM 491

Query: 493 NNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           N +        +A  WG+RYF  NFERL+E+K K DP NFFRHEQSIP
Sbjct: 492 NGKG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Lsi03G001460 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 483.8 bits (1244), Expect = 1.4e-136
Identity = 244/511 (47.75%), Postives = 338/511 (66.14%), Query Frame = 1

Query: 31  NLEDFVNCIYLHSSNNNDSN--STLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIIT 90
           N E F  C+   +SN++  +  S  I    + SYS VL  +IRNLRF+   TPKP  II 
Sbjct: 24  NSETFTQCL---TSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIA 83

Query: 91  PSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYV--ANVPFVLIDLVNLNSITIDTED 150
            +H S VQA + C K H LQ++ RSGGHD++G SYV  +  PF ++D+ NL S+ +D   
Sbjct: 84  ATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVAS 143

Query: 151 ESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNV 210
           ++AWVQ+GA +GE+YY I EKS+TL +PAG  PT+GVGG +SGGG G M+RKYGL  DN 
Sbjct: 144 KTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNT 203

Query: 211 VDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTW 270
           +DA +VD NG++++R  MGEDL+WAI GGGGGS+G+VLA+K+ LV VP  VT F + RT 
Sbjct: 204 IDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTL 263

Query: 271 DQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFISLFLGKATELLALM 330
           +QNA  +IH WQ +AP++  +LFI   +   N +   ++ +  +FI++FLG  T LL+++
Sbjct: 264 EQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSIL 323

Query: 331 EKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEP 390
            + FPELGL + DC E SW++++ F  +  V + S  LLL R  P N   K KSDY  EP
Sbjct: 324 NRRFPELGLVRSDCTETSWIQSVLFWTNIQVGS-SETLLLQRNQPVN-YLKRKSDYVREP 383

Query: 391 ISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQR 450
           IS T LE +W+  K+ ELE   +   P+GG+M  IS   TP PYRAG    I Y   W+ 
Sbjct: 384 ISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD 443

Query: 451 PEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRY 510
                +++E TR+L+ +MTPFVSK+PR ++ NYRD+D+G N+ +G   SY E   +G +Y
Sbjct: 444 ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKY 503

Query: 511 FGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 538
           F  NFERL+++K +VD  NFFR+EQSIP  P
Sbjct: 504 FAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527

BLAST of Lsi03G001460 vs. NCBI nr
Match: gi|659072076|ref|XP_008463307.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 951.8 bits (2459), Expect = 5.3e-274
Identity = 467/541 (86.32%), Postives = 502/541 (92.79%), Query Frame = 1

Query: 1   MEYFWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSS 60
           M+YFW  Y Y  FISLLL  SLATLG G +N+EDFV+CIY+HSSNNNDSNSTLIHTPSSS
Sbjct: 1   MKYFWKCYCY-SFISLLLL-SLATLGCGSNNMEDFVDCIYVHSSNNNDSNSTLIHTPSSS 60

Query: 61  SYSYVLNFSIRNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEG 120
           SYSYVLNFSIRNLRFSEPETPKP+AIITPSH SQVQATVICCKSHGLQ+RTRSGGHDFEG
Sbjct: 61  SYSYVLNFSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEG 120

Query: 121 RSYVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPT 180
           RSYVANVPFVLIDL+NLNSITID EDE+AWVQSGATVGELYY+IGEKS+TLGFPAGF  T
Sbjct: 121 RSYVANVPFVLIDLINLNSITIDVEDETAWVQSGATVGELYYKIGEKSRTLGFPAGFKAT 180

Query: 181 IGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSF 240
           IG+GGFLSGGG+GMMVRKYGL ADNVVDAYVVD NGRVVNRYSMGEDLFWAIRGGGGGSF
Sbjct: 181 IGLGGFLSGGGIGMMVRKYGLGADNVVDAYVVDRNGRVVNRYSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSS 300
           GIVLAWKL+LV VP IVTSFALH+TWDQNAA LI+ WQYIAP VDQDLFISAWVTASNSS
Sbjct: 241 GIVLAWKLRLVQVPSIVTSFALHKTWDQNAANLIYRWQYIAPWVDQDLFISAWVTASNSS 300

Query: 301 -NGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVST 360
            +   RIMEASF SLFLG ATELL+LMEKTFPELGLKK+DCLEMSWVE+MAFSASGFV  
Sbjct: 301 KDESGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKKDCLEMSWVESMAFSASGFVYA 360

Query: 361 ESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMN 420
           +SLELLLDRTP  NGR+K KSDYA EPIS+TAL+GMWERFK EELETVQLILIPFGGKMN
Sbjct: 361 KSLELLLDRTPVDNGRYKTKSDYATEPISKTALKGMWERFKDEELETVQLILIPFGGKMN 420

Query: 421 EISKAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNY 480
           EIS++ETPSP+RAGYPIHIGYY+TWQ+P ADS++LEW RELHDYMTPFVSKSPR AYVNY
Sbjct: 421 EISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYVNY 480

Query: 481 RDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 540
           RDLD+GTNN+DG+P  Y+EAS WGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR
Sbjct: 481 RDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 539

BLAST of Lsi03G001460 vs. NCBI nr
Match: gi|449459146|ref|XP_004147307.1| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 924.1 bits (2387), Expect = 1.2e-265
Identity = 448/510 (87.84%), Postives = 475/510 (93.14%), Query Frame = 1

Query: 32  LEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSH 91
           ++DFVNCIY+HSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKP+AIITPSH
Sbjct: 1   MKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 92  TSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWV 151
            SQVQATVICCKSHGLQ+RTRSGGHDFEGRSYVANVPFVLIDL+NLNSITID EDESAWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDESAWV 120

Query: 152 QSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYV 211
           QSGATVGELY+RIGEKS+TLGFPAGFA TIG+GGFLSGGG GMMVRKYGL ADNVVDAYV
Sbjct: 121 QSGATVGELYFRIGEKSRTLGFPAGFAATIGLGGFLSGGGFGMMVRKYGLGADNVVDAYV 180

Query: 212 VDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAA 271
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP IVTSFALH+ WDQNAA
Sbjct: 181 VDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKIWDQNAA 240

Query: 272 KLIHGWQYIAPEVDQDLFISAWVTASNSSNGGE-RIMEASFISLFLGKATELLALMEKTF 331
            LI+ WQYIAP VDQDLFISAWVTASNSS+ G  RIMEASF SLFLG ATELL+LMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSHDGSGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 332 PELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEPISET 391
           PELGLKKEDCLE SWVE+MAFSASGFVS +SLELLLDRTP  NGR+K KSDYA EPISET
Sbjct: 301 PELGLKKEDCLETSWVESMAFSASGFVSAKSLELLLDRTPLHNGRYKTKSDYATEPISET 360

Query: 392 ALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQRPEAD 451
            LEGMWERFK EELETVQLILIPFGGK NEIS++ETPSP+RAGYPIHIGYY+TWQRP+AD
Sbjct: 361 VLEGMWERFKDEELETVQLILIPFGGKTNEISESETPSPHRAGYPIHIGYYLTWQRPDAD 420

Query: 452 SKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNN 511
           SKHL+W RELH+YMTPFVSKSPR AYVNYRDLD+GTNN+DG+P   EEAS WGHRYFGNN
Sbjct: 421 SKHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNN 480

Query: 512 FERLMEVKMKVDPFNFFRHEQSIPPAPPRV 541
           FERLMEVK KVDPFNFFRHEQSIPPAP  V
Sbjct: 481 FERLMEVKRKVDPFNFFRHEQSIPPAPTSV 510

BLAST of Lsi03G001460 vs. NCBI nr
Match: gi|659068690|ref|XP_008445695.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 622.9 bits (1605), Expect = 5.7e-175
Identity = 303/506 (59.88%), Postives = 392/506 (77.47%), Query Frame = 1

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHT 92
           ++F++C+  HSS +    S +++TP +SSYS VLNFSIRN RFS+PETPKPL IITPSH 
Sbjct: 34  QNFLHCLPEHSSKSYPI-SKVVYTPINSSYSSVLNFSIRNFRFSKPETPKPLLIITPSHV 93

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QA VIC KSHGLQ+RTRSGGHD+EG SYVA  PF+++DL+N+ SI++D  + +AWV+
Sbjct: 94  SHIQAAVICSKSHGLQIRTRSGGHDYEGLSYVAYRPFIIVDLINIRSISVDIVNNTAWVE 153

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           SGAT+GELYYRIGEKS+TL FPAG  PT+GVGG  SGGG G ++RKYGLAADNV+DAY+V
Sbjct: 154 SGATLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGFLLRKYGLAADNVIDAYLV 213

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAK 272
           DANG   +R SMGEDLFWAIRGGGGGSFGIV+AWK+KLVPVP +VT  +  +  + +A K
Sbjct: 214 DANGMAHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPALVTICSTIKNLEGDAVK 273

Query: 273 LIHGWQYIAPEVDQDLFISAWVTASN-SSNGGERIMEASFISLFLGKATELLALMEKTFP 332
           +IH WQY+A ++ +D+F+   +T  N S+ GG +   A+F SLFLGK  EL+A +  TFP
Sbjct: 274 VIHQWQYVANKLHEDIFLGIVLTGGNTSTQGGIKKPTATFYSLFLGKVDELVATLSTTFP 333

Query: 333 ELGLKKEDCLEMSWVEAMAFSASG-FVSTESLELLLDRTPPKNGRHKIKSDYAIEPISET 392
           E GL K+DCLE+ W+E+    ASG F + ESL+ LL+RTP      KIKSDY  +PI + 
Sbjct: 334 EFGLIKKDCLEVRWIESTLIIASGVFQTVESLKPLLNRTPSTIDSTKIKSDYIKKPIPKA 393

Query: 393 ALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW--QRPE 452
           A+EG+W+R K  ++E  QL+ +P+G +M++IS++ETP  +RAGY   IGY V W  Q  +
Sbjct: 394 AIEGIWQRLKAGDIEAPQLVFVPYGARMSQISESETPFSHRAGYLYKIGYMVGWKGQNLK 453

Query: 453 ADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFG 512
           A ++H+ WTREL++YMTPFVSKSPR AYVNYRDLDIG+NN+ G   SY++AS WG +YFG
Sbjct: 454 AKNRHISWTRELYEYMTPFVSKSPRTAYVNYRDLDIGSNNKYG-KTSYKQASIWGLKYFG 513

Query: 513 NNFERLMEVKMKVDPFNFFRHEQSIP 535
           NNF+RL+ VK KVDP +FFRHEQSIP
Sbjct: 514 NNFKRLVYVKNKVDPHDFFRHEQSIP 537

BLAST of Lsi03G001460 vs. NCBI nr
Match: gi|778661997|ref|XP_004151192.2| (PREDICTED: cannabidiolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 620.5 bits (1599), Expect = 2.8e-174
Identity = 302/505 (59.80%), Postives = 384/505 (76.04%), Query Frame = 1

Query: 34  DFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPLAIITPSHTS 93
           +F+ C+  HSS +    S ++HTP +SSYS VLNFSIRNLRFS+PETPKPL IITPSH S
Sbjct: 33  NFLQCLSEHSSKSYPI-SKVVHTPINSSYSSVLNFSIRNLRFSKPETPKPLLIITPSHVS 92

Query: 94  QVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQS 153
            ++A VIC KSHGLQ+RTRSGGHD+EG SYVA+ PF+++DL+NL S+ ID E  +AWV+S
Sbjct: 93  HIRAAVICSKSHGLQIRTRSGGHDYEGLSYVASHPFIILDLINLRSMKIDVESNTAWVES 152

Query: 154 GATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVD 213
           G T+GELYYRIGEKS+TL FPAG  PT+GVGG  SGGG G+M+RK+GLAADNV+DAY+VD
Sbjct: 153 GTTLGELYYRIGEKSRTLAFPAGICPTVGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVD 212

Query: 214 ANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKL 273
           ANG+V +R SMGEDLFWAIRGGGGGSFGIV+AWK+KLV VP  VT    +R    +  KL
Sbjct: 213 ANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVSVPATVTMCNTYRNLKGDGIKL 272

Query: 274 IHGWQYIAPEVDQDLFISAWVTASNSSNGGERIME--ASFISLFLGKATELLALMEKTFP 333
           +H WQY+A ++D++LF+   +T  N +   E I    A+F SLFLGK  EL+A +  TFP
Sbjct: 273 VHQWQYVANKLDENLFLGIILTGGNITTTQEGITNPIATFFSLFLGKVNELVATLSTTFP 332

Query: 334 ELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEPISETA 393
           ELGL K+DC+E SW+E+   +++G  + ESLE LL+RTP      KIKSDY  EPIS   
Sbjct: 333 ELGLIKQDCIETSWIESTLIASTGVQTVESLEPLLNRTPSNLENEKIKSDYIKEPISIAT 392

Query: 394 LEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW--QRPEA 453
           +EG+W+R K +++ET QLI IP+GG+M++IS++ETP  +R G    IGY + W  Q  +A
Sbjct: 393 IEGIWQRLKAQDIETSQLIFIPYGGRMSQISESETPFSHRVGNLYKIGYILGWKEQSLKA 452

Query: 454 DSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGN 513
             KH+ W RE+++YMTPFVSKSPR AY NYRDLDIG N + G   S ++AS WG +YFGN
Sbjct: 453 KKKHISWIREIYEYMTPFVSKSPRSAYANYRDLDIGVNKKYG-KTSVKQASIWGLKYFGN 512

Query: 514 NFERLMEVKMKVDPFNFFRHEQSIP 535
           NF+RL+ VK KVDP++FFRHEQSIP
Sbjct: 513 NFKRLVYVKTKVDPYDFFRHEQSIP 535

BLAST of Lsi03G001460 vs. NCBI nr
Match: gi|659068685|ref|XP_008445674.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 614.4 bits (1583), Expect = 2.0e-172
Identity = 305/528 (57.77%), Postives = 387/528 (73.30%), Query Frame = 1

Query: 11  YCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSI 70
           +  I +L+  S + +       ++F+ C+  HSS      S ++HTP +SSYS VL+FSI
Sbjct: 10  FSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPI-SKVVHTPINSSYSSVLDFSI 69

Query: 71  RNLRFSEPETPKPLAIITPSHTSQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFV 130
           RNL FS+PETPKPL IITPSH S +QA VIC KSHGLQ+RTRSGGHDFEG SYVA  PF+
Sbjct: 70  RNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVAYHPFI 129

Query: 131 LIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGG 190
           ++DL+NL SI ID E  +AWV+SGAT+GELYYRIGEKS+TL FP G  PT+GVGG  SGG
Sbjct: 130 IVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVGVGGHFSGG 189

Query: 191 GLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKL 250
           G G+M+RK+GLAADNV+DAY+VDANG+V +R SMGEDLFWAIRGGGGGSFGIV+AWK+KL
Sbjct: 190 GYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGIVVAWKIKL 249

Query: 251 VPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIME-- 310
           V VP  VT  + +R    +  KL+H WQY+A ++D++LF+   +T  N +   E I    
Sbjct: 250 VSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTTQEGIANPI 309

Query: 311 ASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDR 370
           A+F SLFLGK   L+A +   FPELG  KEDC+E SW+E+   +A+G  + ESLE LL+R
Sbjct: 310 ATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVESLEPLLNR 369

Query: 371 TPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPS 430
           TP      K+KSDY  EPIS   +EG+W+R K +++ET QLI IP+GG+M++IS+ ETP 
Sbjct: 370 TPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQISEMETPF 429

Query: 431 PYRAGYPIHIGYYVTW--QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGT 490
            +RAG    IGY+V W  Q  +A  KH+ W R+++ YMTPFVSKSPR AY NYRDLDIG 
Sbjct: 430 SHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYANYRDLDIGA 489

Query: 491 NNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           N + G   S  +AS WG +YFGNNF+RL+ VK KVDP++FFRHEQSIP
Sbjct: 490 NKKYG-KTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIP 535