Carg27324 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg27324
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Calcium-binding EF-hand family protein, putative
Location	Cucurbita_argyrosperma_scaffold_549 : 3394 .. 3663 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

mRNA sequence

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

Coding sequence (CDS)

ATGTCGTTCTTCCGTCCAAAACTGAATTCGAAGAGCGCCTACTCAACGAAGGAACTGAAAGAGATCTTCCACCAGATGGATACGAATGAAGACGGCGGGTTGGGATTGTTCGAGATCAAGAACGCATTGAAGCAACTCGACGCTGAATTTCCAATCCATCTCGCCTACAAGGCTCTCTGTCACGCCGATCGGAACATAGATGGAAAGCTTGGCGAGGAGGAGATCGATGAATTTGTCAAGTACATGTTGAGCGAAGGCTATGTTGTATGA

Protein sequence

MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV

Homology

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KGN55187.1 (hypothetical protein Csa_4G639750 [Cucumis sativus])

HSP 1 Score: 148.7 bits (374), Expect = 9.6e-33
Identity = 72/95 (75.79%), Postives = 83/95 (87.37%), Query Frame = 0


Query: 2  SFFRPK-------LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHL 61
          S FRPK       ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HL
Sbjct: 3  SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 62 AYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          AY ALCHADRN DGKLGEEEID+FVKYM+S+GYVV
Sbjct: 63 AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: XP_010108598.2 (probable calcium-binding protein CML16 [Morus notabilis])

HSP 1 Score: 69.7 bits (169), Expect = 5.7e-09
Identity = 36/83 (43.37%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 8   LNSKSA-YSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNI 67
           +N K A Y  ++LK +  + DT+ DG L   E+K A K+L A  P   A +ALCHAD+N 
Sbjct: 85  VNPKPAVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRALCHADKNR 144

Query: 68  DGKLGEEEIDEFVKYMLSEGYVV 90
           DG + EEE+   V Y    GYV+
Sbjct: 145 DGFISEEELANLVHYAAQRGYVL 167

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: EXC19753.1 (putative calcium-binding protein CML10 [Morus notabilis] >EXC66137.1 putative calcium-binding protein CML10 [Morus notabilis])

HSP 1 Score: 69.7 bits (169), Expect = 5.7e-09
Identity = 33/78 (42.31%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 12  SAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLG 71
           + Y  ++LK +  + DT+ DG L   E+K A K+L A  P   A +ALCHAD+N DG + 
Sbjct: 23  AVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRALCHADKNRDGFIS 82

Query: 72  EEEIDEFVKYMLSEGYVV 90
           EEE+   V Y    GYV+
Sbjct: 83  EEELANLVHYAAQRGYVL 100

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: OAY22908.1 (hypothetical protein MANES_18G036100 [Manihot esculenta])

HSP 1 Score: 66.6 bits (161), Expect = 4.8e-08
Identity = 36/89 (40.45%), Postives = 51/89 (57.30%), Query Frame = 0


Query: 1  MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALC 60
          M  + PK N    Y+  +LK +F + D N DG L   EIK A ++L +  P     +AL 
Sbjct: 1  MPLYVPK-NVTGTYTEDQLKALFKEHDVNGDGLLSKEEIKKAFQKLGSRLPGWRVNRALH 60

Query: 61 HADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          HAD N DG +G +E+DE VKY++  GY +
Sbjct: 61 HADINGDGNIGLDELDELVKYVIKFGYSI 88

[Close]

BLAST of Carg27324 vs. NCBI nr
Match: KJB14570.1 (hypothetical protein B456_002G131800 [Gossypium raimondii])

HSP 1 Score: 66.2 bits (160), Expect = 6.3e-08
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 17  KELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEID 76
           ++++ IFH+ DTN DG L   E+KNA   L +  P   A+ AL HAD N DG + E E D
Sbjct: 127 EQVRGIFHRYDTNRDGLLSKQELKNAFSSLGSRMPGVRAWLALQHADVNGDGYINEAEFD 186

Query: 77  EFVKYMLSEGY 88
           + VKY L  GY
Sbjct: 187 KLVKYTLKRGY 197

[Close]

BLAST of Carg27324 vs. TrEMBL
Match: tr|A0A0A0L3J7|A0A0A0L3J7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 6.3e-33
Identity = 72/95 (75.79%), Postives = 83/95 (87.37%), Query Frame = 0


Query: 2  SFFRPK-------LNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHL 61
          S FRPK       ++S+SAYS KEL++IF+QMDTNEDGGLGLFEIKNALK+LDAE P+HL
Sbjct: 3  SMFRPKTKSNSPPVSSESAYSAKELRQIFNQMDTNEDGGLGLFEIKNALKKLDAEMPVHL 62

Query: 62 AYKALCHADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          AY ALCHADRN DGKLGEEEID+FVKYM+S+GYVV
Sbjct: 63 AYSALCHADRNRDGKLGEEEIDQFVKYMMSKGYVV 97

[Close]

BLAST of Carg27324 vs. TrEMBL
Match: tr|W9S022|W9S022_9ROSA (Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_000100 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-09
Identity = 33/78 (42.31%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 12  SAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLG 71
           + Y  ++LK +  + DT+ DG L   E+K A K+L A  P   A +ALCHAD+N DG + 
Sbjct: 23  AVYDKEKLKSVIRRYDTDGDGNLSRKELKKAFKELGATAPGWRAIRALCHADKNRDGFIS 82

Query: 72  EEEIDEFVKYMLSEGYVV 90
           EEE+   V Y    GYV+
Sbjct: 83  EEELANLVHYAAQRGYVL 100

[Close]

BLAST of Carg27324 vs. TrEMBL
Match: tr|A0A2C9U150|A0A2C9U150_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G036100 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-08
Identity = 36/89 (40.45%), Postives = 51/89 (57.30%), Query Frame = 0


Query: 1  MSFFRPKLNSKSAYSTKELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALC 60
          M  + PK N    Y+  +LK +F + D N DG L   EIK A ++L +  P     +AL 
Sbjct: 1  MPLYVPK-NVTGTYTEDQLKALFKEHDVNGDGLLSKEEIKKAFQKLGSRLPGWRVNRALH 60

Query: 61 HADRNIDGKLGEEEIDEFVKYMLSEGYVV 90
          HAD N DG +G +E+DE VKY++  GY +
Sbjct: 61 HADINGDGNIGLDELDELVKYVIKFGYSI 88

[Close]

BLAST of Carg27324 vs. TrEMBL
Match: tr|A0A1U8KWD2|A0A1U8KWD2_GOSHI (calcium-binding protein CML39-like OS=Gossypium hirsutum OX=3635 GN=LOC107921375 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.1e-08
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 17  KELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEID 76
           ++++ IFH+ DTN DG L   E+KNA   L +  P   A+ AL HAD N DG + E E D
Sbjct: 50  EQVRGIFHRYDTNRDGLLSKQELKNAFSSLGSRMPGVRAWLALQHADVNGDGYINEAEFD 109

Query: 77  EFVKYMLSEGY 88
           + VKY L  GY
Sbjct: 110 KLVKYTLKRGY 120

[Close]

BLAST of Carg27324 vs. TrEMBL
Match: tr|A0A0D2R5B7|A0A0D2R5B7_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_002G131800 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.1e-08
Identity = 33/71 (46.48%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 17  KELKEIFHQMDTNEDGGLGLFEIKNALKQLDAEFPIHLAYKALCHADRNIDGKLGEEEID 76
           ++++ IFH+ DTN DG L   E+KNA   L +  P   A+ AL HAD N DG + E E D
Sbjct: 127 EQVRGIFHRYDTNRDGLLSKQELKNAFSSLGSRMPGVRAWLALQHADVNGDGYINEAEFD 186

Query: 77  EFVKYMLSEGY 88
           + VKY L  GY
Sbjct: 187 KLVKYTLKRGY 197

The following BLAST results are available for this feature:

BLAST of Carg27324 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN55187.1	9.6e-33	75.79	hypothetical protein Csa_4G639750 [Cucumis sativus]	[more]
XP_010108598.2	5.7e-09	43.37	probable calcium-binding protein CML16 [Morus notabilis]	[more]
EXC19753.1	5.7e-09	42.31	putative calcium-binding protein CML10 [Morus notabilis] >EXC66137.1 putative ca...	[more]
OAY22908.1	4.8e-08	40.45	hypothetical protein MANES_18G036100 [Manihot esculenta]	[more]
KJB14570.1	6.3e-08	46.48	hypothetical protein B456_002G131800 [Gossypium raimondii]	[more]

BLAST of Carg27324 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of Carg27324 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Carg27324 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L3J7\|A0A0A0L3J7_CUCSA	6.3e-33	75.79	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G639750 PE=4 SV=1	[more]
tr\|W9S022\|W9S022_9ROSA	3.7e-09	42.31	Putative calcium-binding protein CML10 OS=Morus notabilis OX=981085 GN=L484_0001...	[more]
tr\|A0A2C9U150\|A0A2C9U150_MANES	3.2e-08	40.45	Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_18G036100 PE=4 SV=...	[more]
tr\|A0A1U8KWD2\|A0A1U8KWD2_GOSHI	4.1e-08	46.48	calcium-binding protein CML39-like OS=Gossypium hirsutum OX=3635 GN=LOC107921375...	[more]
tr\|A0A0D2R5B7\|A0A0D2R5B7_GOSRA	4.1e-08	46.48	Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_002G131800 PE=4 ...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **INTERPRO**
Term	Definition
IPR011992	EF-hand-dom_pair
IPR002048	EF_hand_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27324-RA	Carg27324-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 54..82 e-value: 59.0 score: 3.5 coord: 18..46 e-value: 0.11 score: 21.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 50..85 score: 6.814
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 14..49 score: 11.78
None	No IPR available	GENE3D	G3DSA:1.10.238.10		coord: 2..86 e-value: 8.2E-11 score: 43.7
None	No IPR available	PANTHER	PTHR44455	FAMILY NOT NAMED	coord: 6..82
IPR011992	EF-hand domain pair	SUPERFAMILY	SSF47473	EF-hand	coord: 8..78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg27324	Cla020680	Watermelon (97103) v1	carwmB0836
Carg27324	Csa4G639750	Cucumber (Chinese Long) v2	carcuB0855
Carg27324	MELO3C016747	Melon (DHL92) v3.5.1	carmeB0829
Carg27324	ClCG05G019820	Watermelon (Charleston Gray)	carwcgB0809
Carg27324	CSPI04G24020	Wild cucumber (PI 183967)	carcpiB0875
Carg27324	CmaCh09G002210	Cucurbita maxima (Rimu)	carcmaB1158
Carg27324	CmaCh01G018460	Cucurbita maxima (Rimu)	carcmaB1159
Carg27324	CmoCh09G002150	Cucurbita moschata (Rifu)	carcmoB1128
Carg27324	CmoCh01G019040	Cucurbita moschata (Rifu)	carcmoB1129
Carg27324	Lsi04G015690	Bottle gourd (USVL1VR-Ls)	carlsiB762
Carg27324	Cp4.1LG06g01590	Cucurbita pepo (Zucchini)	carcpeB1168
Carg27324	Cp4.1LG02g07000	Cucurbita pepo (Zucchini)	carcpeB1167
Carg27324	CsGy4G022320	Cucumber (Gy14) v2	carcgybB0829
Carg27324	MELO3C016747.2	Melon (DHL92) v3.6.1	carmedB0841
Carg27324	CsaV3_4G034270	Cucumber (Chinese Long) v3	carcucB0879
Carg27324	Cla97C05G101440	Watermelon (97103) v2	carwmbB0869
Carg27324	Bhi09G002737	Wax gourd	carwgoB0973

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg27324	Carg10598	Silver-seed gourd	carcarB607

The following block(s) are covering this gene:

None