Carg24823 (gene) Silver-seed gourd

NameCarg24823
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description21.7 kDa class VI heat shock protein
LocationCucurbita_argyrosperma_scaffold_157 : 222171 .. 223937 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCTGCCGGCATGGCCACTTCGAAGCCGCAACTGGAAGTCCATAAAGCAGACCACAGCCCTTCAAAATGGAGTGTGGCTCTTGGAGAAGAGGTTTTCAGCAGATTTGTGGGGCAACCCAATTCGGGTGTGCAGAAGGTGTTGGGCGAGGGCTCCTTGTTCAGCCCGCTGCTGTTCGGGAAGTTCTTCGACCCCGCCGATGCCTTTCCGCTCTGGGAGTTTGAGTCCGATCTGTTCATGTCAGCCCTCCGCAGCTCTGGGAAATCTTCAATTGATTGGTTTCAGACTGATCAGGAATACGTTCTTCAAGCAGAATTACAAGGTATTTTCTTCTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCTTTTTTTAATCTTAATATTTAATTTGCAAAATTAATTAAAAAAATTTGTATTTAGATGAAGATGTTAGAGATTTTTTGAAATTTAACTAAGAAGTGAAATTTTTATGCTTAGTTATTAAAATTTTAATTTGGATAAAAACAACTTTTAGGCTGTAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTACTAGAAGTGTTTAATGGATTTTTCAAAATATTAATTATTAGGTGCCAAATTAAATGAATTTTGATTATAAAATTAAAAAAATGGTGAGAGATCAAATTTATTTCAAATCAGAACCTCCCACATCGAAGAATTTGTAAACGATGGAGACTTTAAATTGGTAATCCCGTCCAACGTCCATTGGGGTGTGGGCCAGAAACGACACGCGCTAATTTAAACCCTAAACCTGTTTGACTGTTAAGGGGATTTTGCCGGCGTTTACCCCTGTGGATCCCAAAAAATCTTAAACGTTGATGCACTGCGCGTGGTTTAGTTTCCAGTTGTCTAATATAGCCTTTGAGGTTCGTAGGTGGGTGCTGCCTACACCATTGCCATGGTGTCTTGCGGCGGCAGCTATTGTCCTCGCCTCTTAATCACCCCCCCTTTTTTATTTTTACCAAAATTTTTACTCTTGTGATAGGTCTTTCAATTATAAATTAGACTTTAGAGTCTACTGGACAAAAATTAATTAGTTAGAATCGTATTATATATAAAATTTAAACTTAGGAAAACATATTAAATACAAAATTGAAAGTGAGTTTTAAATTTGACGGTGGTTAAGAGCATTCTCAAACATTTATTTTGCAGAGAGTTGAAGTTGAAAGGATGATAAAAGAGAGAATTGATTTTAATGGTGAAATTATGTTATCAACTTCGTTTTAAAAGACAATCCAAAGGGCCTTCCAACTCTCTCTCTCTCTGTAGCTACTCATTCAAAAAAGATTGGGTGATATGTTTGATTGACACAAACCAAAATTTTACAAACAGAACCTTGGAGAAACGCTTTGCAAATCTCCGTAGAAGACGGGAAGGTGTTAGAGATAAGTGGTCAGTTGAAGGAACAACAAAGAGAAGCCACCAAGCTGGTCGATTGGCGATGTGCCAATTGGTGGGAGCACGGATTCGTTCGGCGACTCGAGTTGCCTGAAGATGTTGATTGTTGTAGAATAGAGGCTCAGATGAAGAATGACACGATTCTTGAGATCAAGATTCCCAAGTTAGAATCGAATAGAGGTGCTGAATCAAAGAGCAAGGATTCTGATCAAGATGTTTAAGTTCATGGCCAAATTTGTAATGGAAAACATGTTTAACTTAAACTTAATTTACATATGCTCTGTGGATCTCAAATATATGTATGTTTCTGACCAACTTCTATGGATGAGAATACAAACACTTTCT

mRNA sequence

CCCCTGCCGGCATGGCCACTTCGAAGCCGCAACTGGAAGTCCATAAAGCAGACCACAGCCCTTCAAAATGGAGTGTGGCTCTTGGAGAAGAGGTTTTCAGCAGATTTGTGGGGCAACCCAATTCGGGTGTGCAGAAGGTGTTGGGCGAGGGCTCCTTGTTCAGCCCGCTGCTGTTCGGGAAGTTCTTCGACCCCGCCGATGCCTTTCCGCTCTGGGAGTTTGAGTCCGATCTGTTCATGTCAGCCCTCCGCAGCTCTGGGAAATCTTCAATTGATTGGTTTCAGACTGATCAGGAATACGTTCTTCAAGCAGAATTACAAGAACCTTGGAGAAACGCTTTGCAAATCTCCGTAGAAGACGGGAAGGTGTTAGAGATAAGTGGTCAGTTGAAGGAACAACAAAGAGAAGCCACCAAGCTGGTCGATTGGCGATGTGCCAATTGGTGGGAGCACGGATTCGTTCGGCGACTCGAGTTGCCTGAAGATGTTGATTGTTGTAGAATAGAGGCTCAGATGAAGAATGACACGATTCTTGAGATCAAGATTCCCAAGTTAGAATCGAATAGAGGTGCTGAATCAAAGAGCAAGGATTCTGATCAAGATGTTTAAGTTCATGGCCAAATTTGTAATGGAAAACATGTTTAACTTAAACTTAATTTACATATGCTCTGTGGATCTCAAATATATGTATGTTTCTGACCAACTTCTATGGATGAGAATACAAACACTTTCT

Coding sequence (CDS)

ATGGCCACTTCGAAGCCGCAACTGGAAGTCCATAAAGCAGACCACAGCCCTTCAAAATGGAGTGTGGCTCTTGGAGAAGAGGTTTTCAGCAGATTTGTGGGGCAACCCAATTCGGGTGTGCAGAAGGTGTTGGGCGAGGGCTCCTTGTTCAGCCCGCTGCTGTTCGGGAAGTTCTTCGACCCCGCCGATGCCTTTCCGCTCTGGGAGTTTGAGTCCGATCTGTTCATGTCAGCCCTCCGCAGCTCTGGGAAATCTTCAATTGATTGGTTTCAGACTGATCAGGAATACGTTCTTCAAGCAGAATTACAAGAACCTTGGAGAAACGCTTTGCAAATCTCCGTAGAAGACGGGAAGGTGTTAGAGATAAGTGGTCAGTTGAAGGAACAACAAAGAGAAGCCACCAAGCTGGTCGATTGGCGATGTGCCAATTGGTGGGAGCACGGATTCGTTCGGCGACTCGAGTTGCCTGAAGATGTTGATTGTTGTAGAATAGAGGCTCAGATGAAGAATGACACGATTCTTGAGATCAAGATTCCCAAGTTAGAATCGAATAGAGGTGCTGAATCAAAGAGCAAGGATTCTGATCAAGATGTTTAA

Protein sequence

MATSKPQLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFDPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIPKLESNRGAESKSKDSDQDV
BLAST of Carg24823 vs. NCBI nr
Match: XP_022985262.1 (21.7 kDa class VI heat shock protein [Cucurbita maxima])

HSP 1 Score: 399.1 bits (1024), Expect = 9.1e-108
Identity = 193/198 (97.47%), Postives = 197/198 (99.49%), Query Frame = 0

Query: 1   MATSKPQLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFD 60
           MATSKPQLEVHKADHSPSKWSVALGEEVFS+FVGQPNSGVQKVLGEGSLFSPLLFGKFFD
Sbjct: 25  MATSKPQLEVHKADHSPSKWSVALGEEVFSKFVGQPNSGVQKVLGEGSLFSPLLFGKFFD 84

Query: 61  PADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL 120
           PADAFPLWEFESDL MSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL
Sbjct: 85  PADAFPLWEFESDLLMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL 144

Query: 121 EISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIPK 180
           EISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEA+MKNDTILEIKIPK
Sbjct: 145 EISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEARMKNDTILEIKIPK 204

Query: 181 LESNRGAESKSKDSDQDV 199
           LE+NRGA+SKSKDSDQDV
Sbjct: 205 LETNRGADSKSKDSDQDV 222

BLAST of Carg24823 vs. NCBI nr
Match: XP_023520414.1 (21.7 kDa class VI heat shock protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.7 bits (1018), Expect = 4.5e-107
Identity = 192/198 (96.97%), Postives = 197/198 (99.49%), Query Frame = 0

Query: 1   MATSKPQLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFD 60
           MATSKPQLEVHKADH+PSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFG+FFD
Sbjct: 1   MATSKPQLEVHKADHNPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGRFFD 60

Query: 61  PADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL 120
           PADAFPLWEFE+DL MSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL
Sbjct: 61  PADAFPLWEFEADLLMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVL 120

Query: 121 EISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIPK 180
           EISGQLKEQQREATKLVDWRCANWWE+GFVRRLELPEDVDCCRIEA+MKNDTILEIKIPK
Sbjct: 121 EISGQLKEQQREATKLVDWRCANWWEYGFVRRLELPEDVDCCRIEARMKNDTILEIKIPK 180

Query: 181 LESNRGAESKSKDSDQDV 199
           LESNRGAESKSKDSDQDV
Sbjct: 181 LESNRGAESKSKDSDQDV 198

BLAST of Carg24823 vs. NCBI nr
Match: ADU55792.1 (HSP23.0 [Citrullus lanatus])

HSP 1 Score: 315.5 bits (807), Expect = 1.3e-82
Identity = 156/199 (78.39%), Postives = 169/199 (84.92%), Query Frame = 0

Query: 1   MATSKPQLEVHKAD-HSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MATSK QLEVHK D H+PSKWSV+LGEEVF +FVGQ NS VQ V G+GSLFSPLLFGKFF
Sbjct: 1   MATSKQQLEVHKEDHHTPSKWSVSLGEEVFRKFVGQANSAVQTVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKV 120
           DPADAFPLWEFESDL +S LR SGKSS+DWFQTDQEY LQAEL E WRNALQIS+EDGKV
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSVDWFQTDQEYGLQAELLEAWRNALQISIEDGKV 120

Query: 121 LEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIP 180
           LEISGQLKEQQRE     DWR  NWWEHG+VRRLELPED D  R+EA+MKND +LEIKIP
Sbjct: 121 LEISGQLKEQQREGRTTFDWRSVNWWEHGYVRRLELPEDADWSRMEARMKNDHVLEIKIP 180

Query: 181 KLESNRGAESKSKD-SDQD 198
           K E+N G ES SKD SD+D
Sbjct: 181 KSETNEGVESNSKDNSDED 199

BLAST of Carg24823 vs. NCBI nr
Match: XP_004152915.1 (PREDICTED: 21.7 kDa class VI heat shock protein isoform X1 [Cucumis sativus] >KGN56157.1 hypothetical protein Csa_3G081340 [Cucumis sativus])

HSP 1 Score: 312.0 bits (798), Expect = 1.5e-81
Identity = 158/200 (79.00%), Postives = 175/200 (87.50%), Query Frame = 0

Query: 1   MATSKPQLEVHKADH-SPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MATSK QLEVHK DH +PSKWSV+LGEEVF RF+GQ NS +QKV G+GSLFSPLLFGKFF
Sbjct: 1   MATSK-QLEVHKEDHPTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISV-EDGK 120
           DPADAFPLWEFESDL +S LR SGKSSIDW QTDQEYVLQAEL E WRNALQIS+ EDGK
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYVLQAELLEAWRNALQISIEEDGK 120

Query: 121 VLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKI 180
           VLEISGQLKEQQRE    VDWR  +WWEHG+VRRLELPED D  R+EA++KND +LEIKI
Sbjct: 121 VLEISGQLKEQQREGKTTVDWRRVHWWEHGYVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLESNRGAESKSKD-SDQD 198
           PKLE+N+G+ESKSKD SD+D
Sbjct: 181 PKLETNQGSESKSKDNSDED 199

BLAST of Carg24823 vs. NCBI nr
Match: XP_008463443.1 (PREDICTED: 21.7 kDa class VI heat shock protein [Cucumis melo])

HSP 1 Score: 308.9 bits (790), Expect = 1.2e-80
Identity = 156/200 (78.00%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MATSKPQLEVHKADHS-PSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MAT K QLEVHK DHS PSKWSV+LGEEVF RF+GQ NS +QKV G+GSLFSPLLFGKFF
Sbjct: 1   MATPK-QLEVHKEDHSTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVED-GK 120
           DPADAFPLWEFESDL +S LR SGKSSIDW QTDQEY+LQAEL E WRNALQIS+E+ GK
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYILQAELLEAWRNALQISIEEGGK 120

Query: 121 VLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKI 180
           VLEISGQL+EQQRE    VDWR  NWWEHGFVRRLELPED D  R+EA++KND +LEIKI
Sbjct: 121 VLEISGQLREQQREGKTTVDWRRVNWWEHGFVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLESNRGAESKSKD-SDQD 198
           PKLE+N+ +ESKSKD SD+D
Sbjct: 181 PKLETNQSSESKSKDNSDED 199

BLAST of Carg24823 vs. TAIR10
Match: AT5G54660.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 194.1 bits (492), Expect = 8.0e-50
Identity = 92/181 (50.83%), Postives = 128/181 (70.72%), Query Frame = 0

Query: 7   QLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFDPADAFP 66
           +LE+H  D +P KWSV LG++VF RF+       + V GEGSLFSP LFGK+FDP+DAFP
Sbjct: 9   KLEIHTDDKTPGKWSVPLGDDVFRRFLSGGGGSEKAVFGEGSLFSPFLFGKYFDPSDAFP 68

Query: 67  LWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVE-DGKVLEISGQ 126
           LWEFE+++ +++LRS G+  +DW QTDQ YVL++++    +N +Q+ V+ +G+V+EISGQ
Sbjct: 69  LWEFEAEVLLASLRSLGQCRVDWSQTDQAYVLKSDIPVVGKNNVQVYVDINGRVMEISGQ 128

Query: 127 LKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKND---TILEIKIPKLE 184
               ++ AT   DWR   WWEHG+VRRLELP D D    EA + N+   + LEI+IPK+ 
Sbjct: 129 WNSNKKAATN-SDWRSGRWWEHGYVRRLELPSDADAKYSEAFLSNNDDYSFLEIRIPKIN 188

BLAST of Carg24823 vs. TAIR10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 63.2 bits (152), Expect = 2.1e-10
Identity = 43/138 (31.16%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 59  FDPADAFPLWE-FESDLFMSALRSS-GKSSIDWFQTDQEYVLQAELQEPWRNALQISVED 118
           FDP  +  +W+ F+   F S+  S+   + +DW +T + +V +A+L    +  +++ +ED
Sbjct: 19  FDPF-SLDVWDPFKELQFPSSSSSAIANARVDWKETAEAHVFKADLPGMKKEEVKVEIED 78

Query: 119 GKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEI 178
             VL+ISG+   ++ E  K   W        GF R+  LPE+V   +++A M+N  +L +
Sbjct: 79  DSVLKISGERHVEKEE--KQDTWHRVERSSGGFSRKFRLPENVKMDQVKASMENG-VLTV 138

Query: 179 KIPKLESN-RGAESKSKD 194
            +PK+E+N + A+ KS D
Sbjct: 139 TVPKVETNKKKAQVKSID 152

BLAST of Carg24823 vs. TAIR10
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 58.5 bits (140), Expect = 5.3e-09
Identity = 45/154 (29.22%), Postives = 71/154 (46.10%), Query Frame = 0

Query: 52  PLLFG----KFFDPADAFPLWEFESDLFMSALRSS--------GKSSIDWFQTDQEYVLQ 111
           P +FG      FDP        FE  L  S L ++          + +DW +T + +V +
Sbjct: 5   PSIFGGRRTNVFDPFSLDVFDPFEGFLTPSGLANAPAMDVAAFTNAKVDWRETPEAHVFK 64

Query: 112 AELQEPWRNALQISVEDGKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDV 171
           A+L    +  +++ VEDG +L+ISG+   +  E  K   W         F RR  LPE+ 
Sbjct: 65  ADLPGLRKEEVKVEVEDGNILQISGERSNENEE--KNDKWHRVERSSGKFTRRFRLPENA 124

Query: 172 DCCRIEAQMKNDTILEIKIPKLESNRGAESKSKD 194
               I+A M+N  +L + +PK+   +  E KS D
Sbjct: 125 KMEEIKASMENG-VLSVTVPKVPEKK-PEVKSID 154

BLAST of Carg24823 vs. TAIR10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 57.8 bits (138), Expect = 9.0e-09
Identity = 44/143 (30.77%), Postives = 80/143 (55.94%), Query Frame = 0

Query: 59  FDPADAFPLWE-FESDLFMSALRSSGKSS------IDWFQTDQEYVLQAELQEPWRNALQ 118
           FDP  +  +W+ F+   F S+L  SG++S      +DW +T + +V +A+L    +  ++
Sbjct: 18  FDPF-SLDVWDPFKELQFPSSL--SGETSAITNARVDWKETAEAHVFKADLPGMKKEEVK 77

Query: 119 ISVEDGKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKND 178
           + +ED  VL+ISG+   ++ E  K   W         F R+ +LPE+V   +++A M+N 
Sbjct: 78  VEIEDDSVLKISGERHVEKEE--KQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENG 137

Query: 179 TILEIKIPKL-ESNRGAESKSKD 194
            +L + +PK+ E+ + A+ KS D
Sbjct: 138 -VLTVTVPKVEEAKKKAQVKSID 154

BLAST of Carg24823 vs. TAIR10
Match: AT3G46230.1 (heat shock protein 17.4)

HSP 1 Score: 57.8 bits (138), Expect = 9.0e-09
Identity = 43/154 (27.92%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 52  PLLFG----KFFDPADAFPLWE-FESDLFMSALRSSGK-------SSIDWFQTDQEYVLQ 111
           P  FG      FDP  +  +W+ FE  L      +  K       + +DW +T + +V +
Sbjct: 5   PSFFGGRRTNVFDPF-SLDVWDPFEGFLTPGLTNAPAKDVAAFTNAKVDWRETPEAHVFK 64

Query: 112 AELQEPWRNALQISVEDGKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDV 171
           A++    +  +++ VEDG +L+ISG+   +  E  K   W         F+RR  LPE+ 
Sbjct: 65  ADVPGLKKEEVKVEVEDGNILQISGERSSENEE--KSDTWHRVERSSGKFMRRFRLPENA 124

Query: 172 DCCRIEAQMKNDTILEIKIPKLESNRGAESKSKD 194
               ++A M+N  +L + +PK++ ++  E KS D
Sbjct: 125 KVEEVKASMENG-VLSVTVPKVQESK-PEVKSVD 153

BLAST of Carg24823 vs. Swiss-Prot
Match: sp|Q9FIT9|HS217_ARATH (21.7 kDa class VI heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP21.7 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.4e-48
Identity = 92/181 (50.83%), Postives = 128/181 (70.72%), Query Frame = 0

Query: 7   QLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFDPADAFP 66
           +LE+H  D +P KWSV LG++VF RF+       + V GEGSLFSP LFGK+FDP+DAFP
Sbjct: 9   KLEIHTDDKTPGKWSVPLGDDVFRRFLSGGGGSEKAVFGEGSLFSPFLFGKYFDPSDAFP 68

Query: 67  LWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVE-DGKVLEISGQ 126
           LWEFE+++ +++LRS G+  +DW QTDQ YVL++++    +N +Q+ V+ +G+V+EISGQ
Sbjct: 69  LWEFEAEVLLASLRSLGQCRVDWSQTDQAYVLKSDIPVVGKNNVQVYVDINGRVMEISGQ 128

Query: 127 LKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKND---TILEIKIPKLE 184
               ++ AT   DWR   WWEHG+VRRLELP D D    EA + N+   + LEI+IPK+ 
Sbjct: 129 WNSNKKAATN-SDWRSGRWWEHGYVRRLELPSDADAKYSEAFLSNNDDYSFLEIRIPKIN 188

BLAST of Carg24823 vs. Swiss-Prot
Match: sp|Q6AUW3|HS223_ORYSJ (22.3 kDa class VI heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP22.3 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-37
Identity = 81/185 (43.78%), Postives = 118/185 (63.78%), Query Frame = 0

Query: 8   LEVHKA--DHSPSKWSVALGEEVFSRFVGQPNSGV-------QKVLGEGSLFSPLLFGKF 67
           +EV +A  D +  +W ++L E  FS F+     GV       + V GEGSLFSP LFGKF
Sbjct: 7   IEVRQAVGDGAAPRWRMSLLENTFSSFLQSIGGGVAADGAAARAVFGEGSLFSPFLFGKF 66

Query: 68  FDPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDG- 127
           FDPADAFPLWEFE ++ ++ALR   ++++DW +TD EY L+A++    +  +++S +D  
Sbjct: 67  FDPADAFPLWEFEPEVLLAALRRGARTTVDWAETDSEYYLRADIPGGRKCDVEVSGDDAM 126

Query: 128 KVLEISGQLKEQQREATKL-VDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKN-DTILE 181
           +V+++SG  +           DWR   WWEHGFVRR+ELPED D  ++EA   + + +LE
Sbjct: 127 RVVDVSGLWRAXXXXXXXXGRDWRAGRWWEHGFVRRVELPEDADWRKVEAFFDDGEGLLE 186

BLAST of Carg24823 vs. Swiss-Prot
Match: sp|P31673|HS174_ORYSJ (17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.4 PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 3.5e-10
Identity = 44/147 (29.93%), Postives = 75/147 (51.02%), Query Frame = 0

Query: 43  VLGEGSLFSPLLFGKFFDPADAFPLWEFESDLFMSALRSSGK---SSIDWFQTDQEYVLQ 102
           ++   ++F P      +DP D FP       LF  A   +     + IDW +T + +V +
Sbjct: 3   MIRRSNVFDPFSL-DLWDPFDGFPFGSGSGSLFPRANSDAAAFAGARIDWKETPEAHVFK 62

Query: 103 AELQEPWRNALQISVEDGKVLEISGQ-LKEQQREATKLVDWRCANWWEHGFVRRLELPED 162
           A++    +  +++ VEDG VL+ISG+ +KEQ+ +  K   W         F+RR  LPE+
Sbjct: 63  ADVPGLKKEEVKVEVEDGNVLQISGERIKEQEEKTDK---WHRVERSSGKFLRRFRLPEN 122

Query: 163 VDCCRIEAQMKNDTILEIKIPKLESNR 186
               +I+A M+N  +L + +PK E  +
Sbjct: 123 TKPEQIKASMENG-VLTVTVPKEEPKK 144

BLAST of Carg24823 vs. Swiss-Prot
Match: sp|P27397|HSP12_DAUCA (18.0 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-09
Identity = 39/129 (30.23%), Postives = 69/129 (53.49%), Query Frame = 0

Query: 59  FDPADAFPLWEFE--SDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVED 118
           +DP   +PL      S  F     +   + IDW +T Q +V +A+L    +  +++ VE+
Sbjct: 24  WDPFQDYPLITSSGTSSEFGKETAAFANTHIDWKETPQAHVFKADLPGLKKEEVKVEVEE 83

Query: 119 GKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEI 178
           GKVL+ISG+  +++ E  K   W    +    F+RR  LPE+ +   ++A M+N  +L +
Sbjct: 84  GKVLQISGERNKEKEE--KNNKWHRVEFSSGKFLRRFRLPENANVDEVKAGMENG-VLTV 143

Query: 179 KIPKLESNR 186
            +PK+E  +
Sbjct: 144 TVPKVEMKK 149

BLAST of Carg24823 vs. Swiss-Prot
Match: sp|Q9XIE3|HS17A_ARATH (17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6A PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.9e-09
Identity = 43/138 (31.16%), Postives = 78/138 (56.52%), Query Frame = 0

Query: 59  FDPADAFPLWE-FESDLFMSALRSS-GKSSIDWFQTDQEYVLQAELQEPWRNALQISVED 118
           FDP  +  +W+ F+   F S+  S+   + +DW +T + +V +A+L    +  +++ +ED
Sbjct: 19  FDPF-SLDVWDPFKELQFPSSSSSAIANARVDWKETAEAHVFKADLPGMKKEEVKVEIED 78

Query: 119 GKVLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEI 178
             VL+ISG+   ++ E  K   W        GF R+  LPE+V   +++A M+N  +L +
Sbjct: 79  DSVLKISGERHVEKEE--KQDTWHRVERSSGGFSRKFRLPENVKMDQVKASMENG-VLTV 138

Query: 179 KIPKLESN-RGAESKSKD 194
            +PK+E+N + A+ KS D
Sbjct: 139 TVPKVETNKKKAQVKSID 152

BLAST of Carg24823 vs. TrEMBL
Match: tr|H6TB45|H6TB45_CITLA (HSP23.0 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 8.7e-83
Identity = 156/199 (78.39%), Postives = 169/199 (84.92%), Query Frame = 0

Query: 1   MATSKPQLEVHKAD-HSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MATSK QLEVHK D H+PSKWSV+LGEEVF +FVGQ NS VQ V G+GSLFSPLLFGKFF
Sbjct: 1   MATSKQQLEVHKEDHHTPSKWSVSLGEEVFRKFVGQANSAVQTVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKV 120
           DPADAFPLWEFESDL +S LR SGKSS+DWFQTDQEY LQAEL E WRNALQIS+EDGKV
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSVDWFQTDQEYGLQAELLEAWRNALQISIEDGKV 120

Query: 121 LEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIP 180
           LEISGQLKEQQRE     DWR  NWWEHG+VRRLELPED D  R+EA+MKND +LEIKIP
Sbjct: 121 LEISGQLKEQQREGRTTFDWRSVNWWEHGYVRRLELPEDADWSRMEARMKNDHVLEIKIP 180

Query: 181 KLESNRGAESKSKD-SDQD 198
           K E+N G ES SKD SD+D
Sbjct: 181 KSETNEGVESNSKDNSDED 199

BLAST of Carg24823 vs. TrEMBL
Match: tr|A0A0A0L6D5|A0A0A0L6D5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G081340 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.6e-82
Identity = 158/200 (79.00%), Postives = 175/200 (87.50%), Query Frame = 0

Query: 1   MATSKPQLEVHKADH-SPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MATSK QLEVHK DH +PSKWSV+LGEEVF RF+GQ NS +QKV G+GSLFSPLLFGKFF
Sbjct: 1   MATSK-QLEVHKEDHPTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISV-EDGK 120
           DPADAFPLWEFESDL +S LR SGKSSIDW QTDQEYVLQAEL E WRNALQIS+ EDGK
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYVLQAELLEAWRNALQISIEEDGK 120

Query: 121 VLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKI 180
           VLEISGQLKEQQRE    VDWR  +WWEHG+VRRLELPED D  R+EA++KND +LEIKI
Sbjct: 121 VLEISGQLKEQQREGKTTVDWRRVHWWEHGYVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLESNRGAESKSKD-SDQD 198
           PKLE+N+G+ESKSKD SD+D
Sbjct: 181 PKLETNQGSESKSKDNSDED 199

BLAST of Carg24823 vs. TrEMBL
Match: tr|A0A1S3CJA6|A0A1S3CJA6_CUCME (21.7 kDa class VI heat shock protein OS=Cucumis melo OX=3656 GN=LOC103501614 PE=3 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 8.2e-81
Identity = 156/200 (78.00%), Postives = 173/200 (86.50%), Query Frame = 0

Query: 1   MATSKPQLEVHKADHS-PSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFF 60
           MAT K QLEVHK DHS PSKWSV+LGEEVF RF+GQ NS +QKV G+GSLFSPLLFGKFF
Sbjct: 1   MATPK-QLEVHKEDHSTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVED-GK 120
           DPADAFPLWEFESDL +S LR SGKSSIDW QTDQEY+LQAEL E WRNALQIS+E+ GK
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYILQAELLEAWRNALQISIEEGGK 120

Query: 121 VLEISGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKI 180
           VLEISGQL+EQQRE    VDWR  NWWEHGFVRRLELPED D  R+EA++KND +LEIKI
Sbjct: 121 VLEISGQLREQQREGKTTVDWRRVNWWEHGFVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLESNRGAESKSKD-SDQD 198
           PKLE+N+ +ESKSKD SD+D
Sbjct: 181 PKLETNQSSESKSKDNSDED 199

BLAST of Carg24823 vs. TrEMBL
Match: tr|V4UMA5|V4UMA5_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009527mg PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.7e-59
Identity = 116/198 (58.59%), Postives = 145/198 (73.23%), Query Frame = 0

Query: 3   TSKPQLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFDPA 62
           TS  +LEVH  D +P KW VALGE+VF RF+ Q N  V K  G+GSLFSPLLFGKFFDP+
Sbjct: 2   TSCIKLEVHTDDRTPHKWIVALGEDVFRRFLSQGNPAVHKAFGDGSLFSPLLFGKFFDPS 61

Query: 63  DAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVLEI 122
           DAFPLWEFESD+ +S L+S+G+SS+DW QTDQ YVL+AEL    +N +Q+SVE+GK++EI
Sbjct: 62  DAFPLWEFESDVLLSHLQSTGQSSVDWLQTDQAYVLKAELPGVGKNQVQVSVENGKIVEI 121

Query: 123 SGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIPK-- 182
           SGQ KEQ+    K  DWR  +WWEHGFVRRLELPED D  + EA + ND  LEI+IPK  
Sbjct: 122 SGQWKEQRDPRAK--DWRSGHWWEHGFVRRLELPEDADWRKTEAYLSNDVFLEIRIPKNP 181

Query: 183 ----LESNRGAESKSKDS 195
               +    GA +K+ ++
Sbjct: 182 STCDISHGNGAATKNSEA 197

BLAST of Carg24823 vs. TrEMBL
Match: tr|V4US93|V4US93_9ROSI (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009527mg PE=3 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.7e-59
Identity = 116/198 (58.59%), Postives = 145/198 (73.23%), Query Frame = 0

Query: 3   TSKPQLEVHKADHSPSKWSVALGEEVFSRFVGQPNSGVQKVLGEGSLFSPLLFGKFFDPA 62
           TS  +LEVH  D +P KW VALGE+VF RF+ Q N  V K  G+GSLFSPLLFGKFFDP+
Sbjct: 2   TSCIKLEVHTDDRTPHKWIVALGEDVFRRFLSQGNPAVHKAFGDGSLFSPLLFGKFFDPS 61

Query: 63  DAFPLWEFESDLFMSALRSSGKSSIDWFQTDQEYVLQAELQEPWRNALQISVEDGKVLEI 122
           DAFPLWEFESD+ +S L+S+G+SS+DW QTDQ YVL+AEL    +N +Q+SVE+GK++EI
Sbjct: 62  DAFPLWEFESDVLLSHLQSTGQSSVDWLQTDQAYVLKAELPGVGKNQVQVSVENGKIVEI 121

Query: 123 SGQLKEQQREATKLVDWRCANWWEHGFVRRLELPEDVDCCRIEAQMKNDTILEIKIPK-- 182
           SGQ KEQ+    K  DWR  +WWEHGFVRRLELPED D  + EA + ND  LEI+IPK  
Sbjct: 122 SGQWKEQRDPRAK--DWRSGHWWEHGFVRRLELPEDADWRKTEAYLSNDVFLEIRIPKNP 181

Query: 183 ----LESNRGAESKSKDS 195
               +    GA +K+ ++
Sbjct: 182 STCDISHGNGAATKNSEA 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022985262.19.1e-10897.4721.7 kDa class VI heat shock protein [Cucurbita maxima][more]
XP_023520414.14.5e-10796.9721.7 kDa class VI heat shock protein [Cucurbita pepo subsp. pepo][more]
ADU55792.11.3e-8278.39HSP23.0 [Citrullus lanatus][more]
XP_004152915.11.5e-8179.00PREDICTED: 21.7 kDa class VI heat shock protein isoform X1 [Cucumis sativus] >KG... [more]
XP_008463443.11.2e-8078.00PREDICTED: 21.7 kDa class VI heat shock protein [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT5G54660.18.0e-5050.83HSP20-like chaperones superfamily protein[more]
AT1G59860.12.1e-1031.16HSP20-like chaperones superfamily protein[more]
AT1G53540.15.3e-0929.22HSP20-like chaperones superfamily protein[more]
AT1G07400.19.0e-0930.77HSP20-like chaperones superfamily protein[more]
AT3G46230.19.0e-0927.92heat shock protein 17.4[more]
Match NameE-valueIdentityDescription
sp|Q9FIT9|HS217_ARATH1.4e-4850.8321.7 kDa class VI heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP21.7 ... [more]
sp|Q6AUW3|HS223_ORYSJ3.3e-3743.7822.3 kDa class VI heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
sp|P31673|HS174_ORYSJ3.5e-1029.9317.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
sp|P27397|HSP12_DAUCA1.3e-0930.2318.0 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1[more]
sp|Q9XIE3|HS17A_ARATH3.9e-0931.1617.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6... [more]
Match NameE-valueIdentityDescription
tr|H6TB45|H6TB45_CITLA8.7e-8378.39HSP23.0 OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
tr|A0A0A0L6D5|A0A0A0L6D5_CUCSA9.6e-8279.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G081340 PE=3 SV=1[more]
tr|A0A1S3CJA6|A0A1S3CJA6_CUCME8.2e-8178.0021.7 kDa class VI heat shock protein OS=Cucumis melo OX=3656 GN=LOC103501614 PE=... [more]
tr|V4UMA5|V4UMA5_9ROSI6.7e-5958.59Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009527mg PE=3 ... [more]
tr|V4US93|V4US93_9ROSI6.7e-5958.59Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009527mg PE=3 ... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR031107HSP20
IPR008978HSP20-like_chaperone
IPR002068A-crystallin/Hsp20_dom
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24823-RACarg24823-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002068Alpha crystallin/Hsp20 domainPFAMPF00011HSP20coord: 88..185
e-value: 2.3E-11
score: 43.6
IPR002068Alpha crystallin/Hsp20 domainPROSITEPS01031SHSPcoord: 78..195
score: 13.049
IPR008978HSP20-like chaperoneGENE3DG3DSA:2.60.40.790coord: 51..190
e-value: 6.3E-20
score: 73.3
IPR008978HSP20-like chaperoneSUPERFAMILYSSF49764HSP20-like chaperonescoord: 52..185
IPR031107Small heat shock protein HSP20PANTHERPTHR11527SMALL HEAT-SHOCK PROTEIN HSP20 FAMILYcoord: 11..193
NoneNo IPR availablePANTHERPTHR11527:SF521.7 KDA CLASS VI HEAT SHOCK PROTEINcoord: 11..193