Carg23727 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg23727
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	Ubiquitin-conjugating enzyme E2
Location	Cucurbita_argyrosperma_scaffold_213 : 175605 .. 177909 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGACGAGGCCGCGAAGGTTGTTGGTGAGTGTTTGGAGCCCCAGTTTTTCCAATTCCACAATTCGACATTTCCTATTTTGTTAGCCTTTTTTTGTTCTTTTTACTGTCAACAACGATTTTAAACTTCTTCGGGATATTGGAGTTTGTTCTAGTTTTGAATCAACTTTTGTTATGATTTTTTCTTATACTATAGAGTGGAAATTGCAAGCTCTGGCGTTAATGTGTGGAAATTTCGTTAGTGAATTCAGATTTTGTTTTATTTAGCTAACTTTGGAATGTTGTACTCCGGAGATGCTTGGATTTTTTTGTTTACTCCATATTGGAAGGTAGTGTGGTGATATGGATAGATGGTTCTATTCTCGTTTCTTTTTCTGATTCGATCAGTTTGGGTCTGCAGGTTGGGCTCTACTAGCTGTTGGTTAAATTCTTGGCGTTTTACTTTTGGCTTTATGTTATGTTGTCTGGATTACCAGACTGTTTGAATGGAGAATCTGTTCGTTTGATTTCCATTCCTATCTTCATGTTAACGAAAGTTGGGCTTCTGACTAAAAAATAAATGTAATGTTATTTCTTCAACAATCTGTAATTTATAGAAAAAGTGTAAAGAAACCCGCAAAAGATTGGGCCCGCCTAATGTAAGTTCATTTTTAGCACCATAAGCTCTAATTGGAACAATATTCTATTTTTTTTTCAGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATGTAAGTTCATTTTTCATCCTTTTGGTTCCTCTTCTTGTGGAGTCTCTGATGGTATTTGGTTCTGCTTTATAAATGTTGATATGGATAGTTACAGACAGCTATGGAAATATCAATTTCTGCACTGTATCAAGTGTAGGATTTCACATAAATGTTTACAAGAAAATTTGTTATCATCAGAATTTAGATAATTGAATTCGAATTGTAGATAACAATGTTTCACAACAATTTGCTTCTCGTATCGACAAATTAGATGCTTTTAGTTTAGGGGTGCGCAGGTTTGGAACCCTATACAAACAAGAGGCTAGCGCGCAATGACTTTCTCTGATAGTGACCGCTCACACTAGTTCGACCTATAGAAAATATCATTTTGTCACAAATACAGGCCGGAATTCCACTAAACCTTGTCCACAGCAGACACTATTTTTGTAGTATAACTTGGACACTTTGACTGGTATTACTAGGGAATACATTTGGAATTAGTTTTGATGTAAAATATTTAAATTGTAGATGATATTGAGAGATTCTGGTTCTTCTTTCATTACTAGTTCCTTTTTTTTTTCTTTTTTTTTTTTCCCTTTTAATCTTCTCGTGTTTATTTTATCTCTCTTTGTTTTCTGATGTTCTCTTGGTACCTTTTGGTTTGGGCCTTATTTTTTGTATGCCGTTTTAATTAATTTGAGAAAAACAAGTCGTTCTCTTCGTTAAAAAGATGTCAATGTCAGACTACTAGGTACATCATTTGAGAATAAGCAACCAGTGTGATAATCTAGCTTATGTGCTTAGACTACAGATTGACCGCAAATCAAGAAAAACCCTATGCCATGTTTATCATCTTTAAATTTTAATATGATTATGAAGATTTGAGCATGTAGTTTTCGGACACTCCAGCCATTTCCGTGCTAAAGGATTGAAATTCAGGAGTTGAAGTATACAAGATCCTGATTTCCTGATTGTATTTAACTTGAAAATTCACAATGAAAGAAAACTGGTGTTTTTGATAACCTCTCATGTTGATAAAAATGATGGCTTCAGCGTTGTAGTGTCATTGCGTCGGTGAAAATTGATGTATTCTTCTTTATTGTTGTTGTTGGGAGGGGATGTAAGAAATTGTAAATTGAATGTTTTCTTCTTGCTTTTCAGACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTATGTTAGGATTTTGTGAAGCACTTTTGCTACAATGTGACTGAAACCAAATTGACCGCTCATGTGAATGTGTAGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

mRNA sequence

ATGGGTGACGAGGCCGCGAAGGTTGTTGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

Coding sequence (CDS)

ATGGGTGACGAGGCCGCGAAGGTTGTTGTTCCTCGAAACTTCAGATTACTGGAAGAACTTGAAAGAGGTGAAAAAGGAATCGGGGATGGAACTGTCAGTTATGGGATGGATGATGCTGATGATATCTGTATGGCTTCTTGGACGGGAACTATTATTGGGCCGCCTAATACTGTTCATGAAGGGCGCATCCATCAACTCAAGCTATTCTGTGGGCAGGATTACCCTGATAATCCTCCGAACGTTAAATTTCAAACTCCGATTAATATGACCTGTGTCAATTCAGAAACCGGGGTGGTGGAACCAAGCCTTTTCCCCATGCTTGCTAACTGGCAAAGGGAGCATACAATGGAGGACATATTAATGCAATTGAAGAAAGAAATGACTTCTCCCCAGAACAGGAGACTAAGTCAGCCTTCTGAA

Protein sequence

MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKKEMTSPQNRRLSQPSE

Homology

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BLAST of Carg23727 vs. NCBI nr
Match: XP_022939181.1 (uncharacterized protein LOC111445176 [Cucurbita moschata])

HSP 1 Score: 296.2 bits (757), Expect = 5.8e-77
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE
Sbjct: 522 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 581

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 582 GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 641

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 642 MQLKKEMTSPQNRRLSQPSE 661

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BLAST of Carg23727 vs. NCBI nr
Match: KGN51442.1 (hypothetical protein Csa_5G547590 [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 1.3e-73
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: XP_011656243.1 (PREDICTED: zinc finger protein VAR3, chloroplastic [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 1.3e-73
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 521 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 580

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 581 GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 640

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 641 MQLKKEMTSPQNRRLSQPSE 660

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BLAST of Carg23727 vs. NCBI nr
Match: XP_016903717.1 (PREDICTED: uncharacterized protein LOC103483035 [Cucumis melo])

HSP 1 Score: 282.7 bits (722), Expect = 6.7e-73
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 565 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 624

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT +NMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 625 GRIYQLKLFCGQDYPDNPPNVRFQTRVNMTCVNPETGVVEPSLFPMLANWQREHTMEDIL 684

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 685 MQLKKEMTSPQNRRLSQPSE 704

[Close]

BLAST of Carg23727 vs. NCBI nr
Match: XP_022990021.1 (ubiquitin-conjugating enzyme E2 variant 1B-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 280.0 bits (715), Expect = 4.3e-72
Identity = 133/140 (95.00%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPP+V+FQT INMTCVN ETGVVEPSLFPML+NWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPSVRFQTRINMTCVNPETGVVEPSLFPMLSNWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. TAIR10
Match: AT1G23260.1 (MMS ZWEI homologue 1)

HSP 1 Score: 235.7 bits (600), Expect = 1.7e-62
Identity = 109/140 (77.86%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           M  E AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GPPNT +E
Sbjct: 1   MSSEEAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG++YP++PP V+FQT INM CVN ETGVVEPSLFPML NW+RE+TMEDIL
Sbjct: 61  GKIFQLKLFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           ++LKKEM +  NR+L+QP E
Sbjct: 121 VKLKKEMMTSHNRKLAQPPE 140

[Close]

BLAST of Carg23727 vs. TAIR10
Match: AT1G70660.1 (MMS ZWEI homologue 2)

HSP 1 Score: 229.9 bits (585), Expect = 9.3e-61
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E  KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +E
Sbjct: 1   MGSEEEKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L
Sbjct: 61  GKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM S QNR+L+QP E
Sbjct: 121 IQLKKEMMSSQNRKLAQPLE 140

[Close]

BLAST of Carg23727 vs. TAIR10
Match: AT3G52560.4 (ubiquitin E2 variant 1D-4)

HSP 1 Score: 211.5 bits (537), Expect = 3.4e-55
Identity = 103/158 (65.19%), Postives = 118/158 (74.68%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPN---- 60
           +G   + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP N    
Sbjct: 3   LGSGGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNIMPL 62

Query: 61  --------------TVHEGRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPS 120
                         TVHEGRI+QLKLFC +DYP+ PP V+F + +NM CVN ETGVV+P 
Sbjct: 63  LLLLVVTEINFMSQTVHEGRIYQLKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPK 122

Query: 121 LFPMLANWQREHTMEDILMQLKKEMTSPQNRRLSQPSE 141
            F +LANWQRE+TMEDIL+QLKKEM++  NR+L QP E
Sbjct: 123 KFGLLANWQREYTMEDILVQLKKEMSTSHNRKLVQPPE 160

[Close]

BLAST of Carg23727 vs. TAIR10
Match: AT2G36060.2 (MMS ZWEI homologue 3)

HSP 1 Score: 210.7 bits (535), Expect = 5.9e-55
Identity = 101/136 (74.26%), Postives = 114/136 (83.82%), Query Frame = 0


Query: 6   AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPN-TVHEGRIH 65
           + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP N TVHEGRI+
Sbjct: 7   SSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNVTVHEGRIY 66

Query: 66  QLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLK 125
           QLKLFC +DYP+ PP V+F + INMTCVN +TGVV+   F +LANWQR++TMEDIL QLK
Sbjct: 67  QLKLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLK 126

Query: 126 KEMTSPQNRRLSQPSE 141
           KEM +  NR+L QP E
Sbjct: 127 KEMAASHNRKLVQPPE 142

[Close]

BLAST of Carg23727 vs. TAIR10
Match: AT5G59300.1 (ubiquitin carrier protein 7)

HSP 1 Score: 52.0 bits (123), Expect = 3.5e-07
Identity = 29/103 (28.16%), Postives = 54/103 (52.43%), Query Frame = 0


Query: 40  DDICMASWTGTIIGPPNTVHEGRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVV 99
           D+  +  W+ TIIGPP+T++EG      +   Q+YP++PP V+F + +    V S+  V 
Sbjct: 67  DEKNIFEWSVTIIGPPDTLYEGGFFNAIMTFPQNYPNSPPTVRFTSDMWHPNVYSDGRVC 126

Query: 100 ---------EPSLFPMLA-NWQREHTMEDILMQLKKEMTSPQN 133
                    +PS + + +  W   HT+E I++ +   ++ P +
Sbjct: 127 ISILHPPGDDPSGYELASERWTPVHTVESIMLSIISMLSGPND 169

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BLAST of Carg23727 vs. Swiss-Prot
Match: sp|Q93YP0|UEV1A_ARATH (Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UEV1A PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.1e-61
Identity = 109/140 (77.86%), Postives = 124/140 (88.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           M  E AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GPPNT +E
Sbjct: 1   MSSEEAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG++YP++PP V+FQT INM CVN ETGVVEPSLFPML NW+RE+TMEDIL
Sbjct: 61  GKIFQLKLFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           ++LKKEM +  NR+L+QP E
Sbjct: 121 VKLKKEMMTSHNRKLAQPPE 140

[Close]

BLAST of Carg23727 vs. Swiss-Prot
Match: sp|Q9CAB6|UEV1B_ARATH (Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UEV1B PE=1 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-59
Identity = 107/140 (76.43%), Postives = 122/140 (87.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E  KVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTI+GP NT +E
Sbjct: 1   MGSEEEKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           G+I QLKLFCG+DYP++PP V+FQ+ INM CVN E GVV+PS FPML+NW+RE TMED+L
Sbjct: 61  GKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM S QNR+L+QP E
Sbjct: 121 IQLKKEMMSSQNRKLAQPLE 140

[Close]

BLAST of Carg23727 vs. Swiss-Prot
Match: sp|Q9SVD7|UEV1D_ARATH (Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UEV1D PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 2.7e-57
Identity = 103/140 (73.57%), Postives = 118/140 (84.29%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           +G   + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP NTVHE
Sbjct: 3   LGSGGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHE 62

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFC +DYP+ PP V+F + +NM CVN ETGVV+P  F +LANWQRE+TMEDIL
Sbjct: 63  GRIYQLKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDIL 122

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           +QLKKEM++  NR+L QP E
Sbjct: 123 VQLKKEMSTSHNRKLVQPPE 142

[Close]

BLAST of Carg23727 vs. Swiss-Prot
Match: sp|Q9SJ44|UEV1C_ARATH (Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UEV1C PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 4.3e-55
Identity = 101/135 (74.81%), Postives = 114/135 (84.44%), Query Frame = 0


Query: 6   AKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQ 65
           + VVVPRNFRLLEELERGEKGIGDGTVSYGMDD DDI M SWTGTIIGP NTVHEGRI+Q
Sbjct: 7   SSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQ 66

Query: 66  LKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKK 125
           LKLFC +DYP+ PP V+F + INMTCVN +TGVV+   F +LANWQR++TMEDIL QLKK
Sbjct: 67  LKLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKK 126

Query: 126 EMTSPQNRRLSQPSE 141
           EM +  NR+L QP E
Sbjct: 127 EMAASHNRKLVQPPE 141

[Close]

BLAST of Carg23727 vs. Swiss-Prot
Match: sp|Q54D06|UBE2V_DICDI (Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=44689 GN=ube2v PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 3.3e-39
Identity = 70/131 (53.44%), Postives = 98/131 (74.81%), Query Frame = 0


Query: 10  VPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHEGRIHQLKLF 69
           +PRNF LLEELE GEK  GDGT+SYG++  DDI ++SW GTIIGP NT  + R + LKL+
Sbjct: 4   IPRNFVLLEELESGEKSTGDGTISYGLESPDDILLSSWIGTIIGPQNTPFDNRFYSLKLY 63

Query: 70  CGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDILMQLKKEMTS 129
           C +DYP+ PP V+F T IN+ CVN + GVV+P   P+L+NW  ++ +++IL +L++EM+S
Sbjct: 64  CDKDYPNKPPTVRFTTKINIGCVNQQNGVVDPKNLPILSNWTSKNRIQNILSELRREMSS 123

Query: 130 PQNRRLSQPSE 141
             N++L QP E
Sbjct: 124 SSNKKLVQPPE 134

[Close]

BLAST of Carg23727 vs. TrEMBL
Match: tr|A0A0A0KSP5|A0A0A0KSP5_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G547590 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 8.9e-74
Identity = 136/140 (97.14%), Postives = 138/140 (98.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT INMTCVNSETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPNVRFQTRINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 121 MQLKKEMTSPQNRRLSQPSE 140

[Close]

BLAST of Carg23727 vs. TrEMBL
Match: tr|A0A1S4E671|A0A1S4E671_CUCME (uncharacterized protein LOC103483035 OS=Cucumis melo OX=3656 GN=LOC103483035 PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 4.4e-73
Identity = 134/140 (95.71%), Postives = 137/140 (97.86%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI MASWTGTIIGPPNTVHE
Sbjct: 565 MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMASWTGTIIGPPNTVHE 624

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPPNV+FQT +NMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 625 GRIYQLKLFCGQDYPDNPPNVRFQTRVNMTCVNPETGVVEPSLFPMLANWQREHTMEDIL 684

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
           MQLKKEMTSPQNRRLSQPSE
Sbjct: 685 MQLKKEMTSPQNRRLSQPSE 704

[Close]

BLAST of Carg23727 vs. TrEMBL
Match: tr|A0A2P4K007|A0A2P4K007_QUESU (Ubiquitin-conjugating enzyme e2 variant 1b OS=Quercus suber OX=58331 GN=CFP56_51116 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 3.6e-67
Identity = 124/140 (88.57%), Postives = 129/140 (92.14%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E + VVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTIIGPPNTVHE
Sbjct: 1   MGSEGSSVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCG+DYPDNPP V+FQT INMTCVN ETGVVEPSLFPMLANWQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGKDYPDNPPTVRFQTRINMTCVNQETGVVEPSLFPMLANWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
            QLKKEM SPQNR+LSQP E
Sbjct: 121 TQLKKEMMSPQNRKLSQPPE 140

[Close]

BLAST of Carg23727 vs. TrEMBL
Match: tr|A5B7Q8|A5B7Q8_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0011g05630 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-66
Identity = 124/140 (88.57%), Postives = 128/140 (91.43%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E + VVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTIIGPPNTVHE
Sbjct: 1   MGSEGSGVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIYMQSWTGTIIGPPNTVHE 60

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCGQDYPDNPP VKFQT INMTCVN ETGVVEPSLFPMLA WQREHTMEDIL
Sbjct: 61  GRIYQLKLFCGQDYPDNPPTVKFQTRINMTCVNQETGVVEPSLFPMLATWQREHTMEDIL 120

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
            QLKKEM SPQNR+L+QP E
Sbjct: 121 TQLKKEMMSPQNRKLAQPPE 140

[Close]

BLAST of Carg23727 vs. TrEMBL
Match: tr|A0A061E9M9|A0A061E9M9_THECC (Mms zwei OS=Theobroma cacao OX=3641 GN=TCM_011026 PE=4 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.8e-66
Identity = 122/140 (87.14%), Postives = 131/140 (93.57%), Query Frame = 0


Query: 1   MGDEAAKVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDICMASWTGTIIGPPNTVHE 60
           MG E ++VVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDI M SWTGTIIGPPNTVHE
Sbjct: 118 MGSEGSRVVVPRNFRLLEELERGEKGIGDGTVSYGMDDADDIFMQSWTGTIIGPPNTVHE 177

Query: 61  GRIHQLKLFCGQDYPDNPPNVKFQTPINMTCVNSETGVVEPSLFPMLANWQREHTMEDIL 120
           GRI+QLKLFCG+DYPDNPP+V+FQT INMTCVN ETGVVEPSLFPMLANWQRE+TMEDIL
Sbjct: 178 GRIYQLKLFCGKDYPDNPPSVRFQTRINMTCVNQETGVVEPSLFPMLANWQREYTMEDIL 237

Query: 121 MQLKKEMTSPQNRRLSQPSE 141
            QLKKEM SPQNR+L+QP E
Sbjct: 238 TQLKKEMMSPQNRKLAQPPE 257

The following BLAST results are available for this feature:

BLAST of Carg23727 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022939181.1	5.8e-77	100.00	uncharacterized protein LOC111445176 [Cucurbita moschata]	[more]
KGN51442.1	1.3e-73	97.14	hypothetical protein Csa_5G547590 [Cucumis sativus]	[more]
XP_011656243.1	1.3e-73	97.14	PREDICTED: zinc finger protein VAR3, chloroplastic [Cucumis sativus]	[more]
XP_016903717.1	6.7e-73	95.71	PREDICTED: uncharacterized protein LOC103483035 [Cucumis melo]	[more]
XP_022990021.1	4.3e-72	95.00	ubiquitin-conjugating enzyme E2 variant 1B-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg23727 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G23260.1	1.7e-62	77.86	MMS ZWEI homologue 1	[more]
AT1G70660.1	9.3e-61	76.43	MMS ZWEI homologue 2	[more]
AT3G52560.4	3.4e-55	65.19	ubiquitin E2 variant 1D-4	[more]
AT2G36060.2	5.9e-55	74.26	MMS ZWEI homologue 3	[more]
AT5G59300.1	3.5e-07	28.16	ubiquitin carrier protein 7	[more]

BLAST of Carg23727 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q93YP0\|UEV1A_ARATH	3.1e-61	77.86	Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
sp\|Q9CAB6\|UEV1B_ARATH	1.7e-59	76.43	Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
sp\|Q9SVD7\|UEV1D_ARATH	2.7e-57	73.57	Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
sp\|Q9SJ44\|UEV1C_ARATH	4.3e-55	74.81	Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana OX=3702 GN=UE...	[more]
sp\|Q54D06\|UBE2V_DICDI	3.3e-39	53.44	Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum OX=...	[more]

BLAST of Carg23727 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KSP5\|A0A0A0KSP5_CUCSA	8.9e-74	97.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G547590 PE=4 SV=1	[more]
tr\|A0A1S4E671\|A0A1S4E671_CUCME	4.4e-73	95.71	uncharacterized protein LOC103483035 OS=Cucumis melo OX=3656 GN=LOC103483035 PE=...	[more]
tr\|A0A2P4K007\|A0A2P4K007_QUESU	3.6e-67	88.57	Ubiquitin-conjugating enzyme e2 variant 1b OS=Quercus suber OX=58331 GN=CFP56_51...	[more]
tr\|A5B7Q8\|A5B7Q8_VITVI	1.1e-66	88.57	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_01s0011g05630 PE=4 SV=...	[more]
tr\|A0A061E9M9\|A0A061E9M9_THECC	1.8e-66	87.14	Mms zwei OS=Theobroma cacao OX=3641 GN=TCM_011026 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000608	UBQ-conjugat_E2
IPR016135	UBQ-conjugating_enzyme/RWD

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg23727-RA	Carg23727-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 14..140 e-value: 3.2E-6 score: 36.6
None	No IPR available	PANTHER	PTHR43915	FAMILY NOT NAMED	coord: 5..140
None	No IPR available	PANTHER	PTHR43915:SF4	UBIQUITIN-CONJUGATING ENZYME E2 VARIANT 1D	coord: 5..140
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	G3DSA:3.10.110.10		coord: 1..140 e-value: 1.2E-59 score: 202.3
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	SSF54495	UBC-like	coord: 9..138
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 41..133 e-value: 1.9E-15 score: 56.7
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 14..140 score: 18.051

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg23727	Cla003950	Watermelon (97103) v1	carwmB0954
Carg23727	Csa5G547590	Cucumber (Chinese Long) v2	carcuB0968
Carg23727	MELO3C005277	Melon (DHL92) v3.5.1	carmeB0933
Carg23727	ClCG01G006500	Watermelon (Charleston Gray)	carwcgB0928
Carg23727	Cucsa.012160	Cucumber (Gy14) v1	carcgyB1071
Carg23727	CmaCh15G009760	Cucurbita maxima (Rimu)	carcmaB1305
Carg23727	CmaCh04G021100	Cucurbita maxima (Rimu)	carcmaB1306
Carg23727	CmoCh04G022110	Cucurbita moschata (Rifu)	carcmoB1275
Carg23727	CmoCh15G010230	Cucurbita moschata (Rifu)	carcmoB1274
Carg23727	Cp4.1LG01g18290	Cucurbita pepo (Zucchini)	carcpeB1319
Carg23727	Cp4.1LG13g03520	Cucurbita pepo (Zucchini)	carcpeB1318
Carg23727	CsGy5G018720	Cucumber (Gy14) v2	carcgybB0940
Carg23727	MELO3C005277.2	Melon (DHL92) v3.6.1	carmedB0939
Carg23727	CsaV3_5G028100	Cucumber (Chinese Long) v3	carcucB0996
Carg23727	Cla97C01G006810	Watermelon (97103) v2	carwmbB1002
Carg23727	Bhi12G000814	Wax gourd	carwgoB1104

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Carg23727	Carg26059	Silver-seed gourd	carcarB440

The following block(s) are covering this gene:


Gene	Organism	Block
Carg23727	Cucumber (Gy14) v2	carcgybB0941
Carg23727	Wild cucumber (PI 183967)	carcpiB0990
Carg23727	Cucumber (Chinese Long) v2	carcuB0969
Carg23727	Bottle gourd (USVL1VR-Ls)	carlsiB868