Carg19489 (gene) Silver-seed gourd

NameCarg19489
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
LocationCucurbita_argyrosperma_scaffold_204 : 448166 .. 449679 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGTTGTTTTTCCGAATTTTGATTCTGATTCCATTACATTTCATTAGATTCTCACTCGCTAACGAATTTTGGAGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGTAATTCACTCAAACACGTCGCTAATTCACTCTGTTGTATCGAATGATCACACGATATTTAATTTACCGAAGAAACTAAAACTCAGTTTCGTTCTAATTTTCAGAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGCAAGAACATTACAGAACAATTTTATTTTCATCTCCTTTAATCACGAGGCCTTTTACCATTATTGACGAACCGATGATGCAGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGGTATGAATAATGATTAATTTCCCTTCGCTTATGCATTACTGGCGGTTTGATCATAGCGACAGTGATATTCACTTGCAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGTTCTTCAATACATCACATTCATTGGCATTTTAATTGAATGATTTCACGTCCGCGTTGGATTTTGACACAATCGAAACAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGGTACGAACTACCGAACAATTAAGCTCCTGCATGGAAACTAATCGATCTAAAAAGCGTATACAGATAATTAATTCAATGAGAATGCAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

mRNA sequence

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Coding sequence (CDS)

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Protein sequence

MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
BLAST of Carg19489 vs. NCBI nr
Match: XP_022923251.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 646.4 bits (1666), Expect = 5.5e-182
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Carg19489 vs. NCBI nr
Match: XP_023552712.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.7 bits (1659), Expect = 3.6e-181
Identity = 332/334 (99.40%), Postives = 333/334 (99.70%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Carg19489 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 641.7 bits (1654), Expect = 1.4e-180
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Carg19489 vs. NCBI nr
Match: XP_023552711.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.0 bits (1647), Expect = 8.8e-180
Identity = 332/335 (99.10%), Postives = 333/335 (99.40%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

BLAST of Carg19489 vs. NCBI nr
Match: XP_022965266.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 632.1 bits (1629), Expect = 1.1e-177
Identity = 324/334 (97.01%), Postives = 325/334 (97.31%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNF    ESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

BLAST of Carg19489 vs. TAIR10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 416.0 bits (1068), Expect = 2.2e-116
Identity = 219/327 (66.97%), Postives = 263/327 (80.43%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  KYL+G+AGASG+GSKSTAEEV+E+C + SITA++TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRSITAVITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLL 120
           +I PARN+KAAEEAK RIVSE   F  + I VM LDLSS+ SV NF    ESL+LPLNLL
Sbjct: 61  LIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG+  +EHA SEDGIEMTFATNYLGHFLLT LLLN+MI+TA+ TGVQGRIVNVTS I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           H WFSGD+ EYL  ISQ    +D+TRAYA SKLANVLHT +L+ +LQ++ ANVTVNCVHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVV+T L R+REG +TDL+F++ASK  L+KT+P+ AAT+CYVATN  + NV+G YF DCN
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASK--LVKTVPQAAATTCYVATNPRLVNVSGKYFTDCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVS 327
           +       T S+  A  LW+ASE +V+
Sbjct: 301 ETTPSGLGTNSSE-ATKLWAASEILVT 321

BLAST of Carg19489 vs. TAIR10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 400.6 bits (1028), Expect = 9.5e-112
Identity = 209/332 (62.95%), Postives = 259/332 (78.01%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVS-ESCMNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  K+L+GS G SG+GS+STA+ V+  S + S+TAI+TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRSLTAIITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLL 120
           +++PAR++K AEE KARI+SE   F  + I VM LDLSS+ SV  F    ESLNLPLN+L
Sbjct: 61  LVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG++ ++HA SEDG+EMTFATNYLGHFLLTKLLL +MIETA  TGVQGRIVNVTS +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           HSWFSGD+ +YL  IS+ NR YD+TRAYA SKLANVLHTV+L+  L +M ANVT NCVHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           G+VKT L R+REG VTDL+F++ SK  LLK++P+ AAT+CYVAT+  + NV G YF+DCN
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSK--LLKSVPQAAATTCYVATSPRLRNVCGKYFSDCN 300

Query: 301 D-----QGSCECKTKSAHLAEGLWSASETIVS 327
           +      GSC  K      A+ LW+AS+ +VS
Sbjct: 301 EARSSKSGSCNLK------AQRLWTASDLLVS 321

BLAST of Carg19489 vs. TAIR10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 285.4 bits (729), Expect = 4.5e-77
Identity = 154/323 (47.68%), Postives = 223/323 (69.04%), Query Frame = 0

Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINN 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F     S +LPLN+LINN
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 185
           AG F     FSE+ IE+TFATN+LGH+LLT++L+ +MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 245
              D F +   +   +R Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+V
Sbjct: 186 VKPDCFSFPKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGIV 245

Query: 246 KTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQG 305
           KT + R  +G  TD +F +ASK  LLK+I +GAAT+CYVA +   + ++G YFADCN+  
Sbjct: 246 KTGIIRAHKGLFTDSLFLIASK--LLKSISQGAATTCYVALSNETKGLSGKYFADCNET- 305

Query: 306 SCECKTKSAHLAEGLWSASETIV 326
           +C       ++A  L + S  ++
Sbjct: 306 NCSDLANDEYVALKLCTQSRALI 321

BLAST of Carg19489 vs. TAIR10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 261.2 bits (666), Expect = 9.0e-70
Identity = 150/331 (45.32%), Postives = 216/331 (65.26%), Query Frame = 0

Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAEEV++     ++TAI+TG T GIG ETARVL+KRG  ++I
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVI 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINN 123
            ARN+ AAE AK  I+ ++    ++R+T++ LDLSS+ S+  F     +L+LPLNLLINN
Sbjct: 63  GARNMGAAENAKTEILRQN---ANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINN 122

Query: 124 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTS--NIH 183
           AG     +  SEDGIE+ FATN++GHFLLT LLL+ M  TAK++GV+GRI+NV+S  +I+
Sbjct: 123 AGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIY 182

Query: 184 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 243
           ++  G  F+ +  I      Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHPG
Sbjct: 183 TYQEGIQFDSINDICS----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPG 242

Query: 244 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 303
           ++ TNL +     +  L F+      L K IP+GAAT+CYVA + +V+ V G YFADCN+
Sbjct: 243 LILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNE 302

Query: 304 QGSCECKTKSAHLAEGLWSASETIVSTLTGK 332
               +   +   LA+ LW  S  ++++++ K
Sbjct: 303 VTPSKL-ARDETLAQKLWDFSVKLINSVSKK 322

BLAST of Carg19489 vs. TAIR10
Match: AT2G37540.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 245.7 bits (626), Expect = 3.9e-65
Identity = 144/327 (44.04%), Postives = 208/327 (63.61%), Query Frame = 0

Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAE+V+++   + +TAI+TG TSGIG E ARVLA RG  +II
Sbjct: 3   IYGVMTGKKGKSGFGSASTAEDVTQAIDASHLTAIITGGTSGIGLEAARVLAMRGAHVII 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINN 123
            ARN KAA E+K  I+  +    ++R+  + +D+SS+ SV +F     +LN+PLN+LINN
Sbjct: 63  AARNPKAANESKEMILQMN---PNARVDYLQIDVSSIKSVRSFVDQFLALNVPLNILINN 122

Query: 124 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 183
           AG        +EDGIE  FATN++GHFLLT LLL++M  TA+ +GVQGRIVN++S  H++
Sbjct: 123 AGVMFCPFKLTEDGIESQFATNHIGHFLLTNLLLDKMKSTARESGVQGRIVNLSSIAHTY 182

Query: 184 FSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 243
              +  ++ G        Y   RAY  SKL+N+LH+  L+ +LQE   N+T+N VHPG+V
Sbjct: 183 TYSEGIKFQGINDPAG--YSERRAYGQSKLSNLLHSNALSRRLQEEGVNITINSVHPGLV 242

Query: 244 KTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQG 303
            TNL R   GF   +  + A      K IP+GAAT+CYVA + ++E V G YF DCN   
Sbjct: 243 TTNLFR-YSGF--SMKVFRAMTFLFWKNIPQGAATTCYVALHPDLEGVTGKYFGDCNIVA 302

Query: 304 SCECKTKSAHLAEGLWSASETIVSTLT 330
             +  T ++ LA+ LW  S  ++ +++
Sbjct: 303 PSKFATNNS-LADKLWDFSVFLIDSIS 320

BLAST of Carg19489 vs. Swiss-Prot
Match: sp|A2RVM0|TIC32_ARATH (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.1e-59
Identity = 143/328 (43.60%), Postives = 197/328 (60.06%), Query Frame = 0

Query: 7   YLVGSAGASGYGSKSTAEEVSESCMNS-ITAIVTGATSGIGEETARVLAKRGVRIIIPAR 66
           +  GS GASG+ S+STAEEV+     + +TAIVTGA+SGIG ETARVL+ RGV +++  R
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 61

Query: 67  NLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINNAGR 126
           N  +  + K  IV +      +++ VM LDLSS+ SV  F    +S  LPLNLLINNAG 
Sbjct: 62  NTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGI 121

Query: 127 FCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH--SWF 186
                  S+D IE+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H  S+ 
Sbjct: 122 MACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYP 181

Query: 187 SGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVK 246
            G  F+ +   S     Y S RAY  SKL NVLH  +L  +L+E   N+T N +HPG + 
Sbjct: 182 EGVRFDKINDKSS----YSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIM 241

Query: 247 TNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGS 306
           TNL R    ++   +  +A    +LK++P+GAAT+CYVA N  V  V+G YF D N    
Sbjct: 242 TNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKP 301

Query: 307 CECKTKSAHLAEGLWSASETIVSTLTGK 332
                K   LA+ +W  S  +  + +G+
Sbjct: 302 LPL-VKDTELAKKVWDFSTKLTDSQSGE 319

BLAST of Carg19489 vs. Swiss-Prot
Match: sp|Q6RVV4|TIC32_PEA (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.7e-54
Identity = 135/316 (42.72%), Postives = 185/316 (58.54%), Query Frame = 0

Query: 13  GASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAE 72
           G SG+   STAE+V+       +TAIVTGA+SGIG ET RVLA RG  +I+  RN+ AA+
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAAK 68

Query: 73  EAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINNAGRFCYEHA 132
           + K  I+ +      +++  + LDLSS++SV  F     S   PLN+LINNAG       
Sbjct: 69  DVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 FSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH--SWFSGDIFE 192
            S+D IE+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H  ++  G  F+
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFD 188

Query: 193 YLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE 252
            +   S     Y++ RAY  SKLANVLH  QL   L+E   N+T N +HPG + TNL R 
Sbjct: 189 KINDQSS----YNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRH 248

Query: 253 REGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTK 312
               V  LI  +     +LK + +GAAT+CYVA +  V+ V+G YF+D N   +     K
Sbjct: 249 NSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTP-HGK 308

Query: 313 SAHLAEGLWSASETIV 326
              LA+ LW  S  +V
Sbjct: 309 DVDLAKKLWDFSINLV 313

BLAST of Carg19489 vs. Swiss-Prot
Match: sp|Q9HBH5|RDH14_HUMAN (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 6.7e-38
Identity = 118/310 (38.06%), Postives = 168/310 (54.19%), Query Frame = 0

Query: 31  MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSE----SDCFDH 90
           M+  T ++TGA SG+G  TA  L + G R+I+  R+   AEEA  ++  E    ++C   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 91  ------SRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINNAGRFCYEHAFSEDGIEMT 150
                   + V  LDL+S+ SV  F          L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 151 FATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDI-FEYLGQISQKNR 210
           F  N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  GDI F+ L       +
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDL----NSEQ 220

Query: 211 VYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE-REGFVTDLI 270
            Y+ +  Y+ SKLAN+L T +LA +L+    NVTVN +HPG+V+TNL R      +   +
Sbjct: 221 SYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPL 280

Query: 271 FYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLW 329
           F + S +   KT  EGA TS Y+A++  VE V+G YF DC ++     K     +A  LW
Sbjct: 281 FNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLP-KAMDESVARKLW 335

BLAST of Carg19489 vs. Swiss-Prot
Match: sp|Q9ERI6|RDH14_MOUSE (Retinol dehydrogenase 14 OS=Mus musculus OX=10090 GN=Rdh14 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 8.7e-38
Identity = 119/308 (38.64%), Postives = 168/308 (54.55%), Query Frame = 0

Query: 31  MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSE--------SD 90
           M+  T ++TGA SG+G  TA  L + G R+I+  R+   AEEA  ++  E         D
Sbjct: 42  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRQELCQAGGAGPD 101

Query: 91  CFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNLLINNAGRFCYEHAFSEDGIEMTFA 150
             D  ++ V  LDL+S+ SV  F          L++LINNAG F   +  +EDG EM F 
Sbjct: 102 GTD-GQLVVKELDLASLRSVRAFCQELLQEEPRLDVLINNAGVFHCPYTKTEDGFEMQFG 161

Query: 151 TNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDI-FEYLGQISQKNRVY 210
            N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  G+I FE L       + Y
Sbjct: 162 VNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GEINFEDL----NSEQSY 221

Query: 211 DSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE-REGFVTDLIFY 270
           + +  Y+ SKLAN+L T +LA +L+    NVTVN +HPG+V+TNL R      +   +F 
Sbjct: 222 NKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLARPLFN 281

Query: 271 MASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSA 329
           + S +   KT  EGA TS Y+A + +VE V+G YF DC ++     K     +A  LW  
Sbjct: 282 LVSWA-FFKTPLEGAQTSIYLACSPDVEGVSGRYFGDCKEEELLP-KAMDESVARKLWDI 333

BLAST of Carg19489 vs. Swiss-Prot
Match: sp|P59837|RDH12_BOVIN (Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.4e-35
Identity = 108/289 (37.37%), Postives = 162/289 (56.06%), Query Frame = 0

Query: 37  IVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDL 96
           ++TGA +GIG+ETAR LA+RG R+ I  R++   E A + I +++    +S++ V  LDL
Sbjct: 43  VITGANTGIGKETARELARRGARVYIACRDVLKGESAASEIQADT---KNSQVLVRKLDL 102

Query: 97  SSVNSVVNFXXXXESLNLPLNLLINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLL 156
           S   S+  F     +    L++LINNAG     ++ + DG E   A N+LGHFLLT LLL
Sbjct: 103 SDTKSIRAFAEGFLAEEKQLHILINNAGVMLCPYSKTADGFETHLAVNHLGHFLLTHLLL 162

Query: 157 NRMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYLGQIS----QKNRVYDSTRAYAFSKL 216
            R+ E+A +     R+VN++S  H         +LG+I     Q ++ Y+   AY  SKL
Sbjct: 163 GRLKESAPA-----RVVNLSSVAH---------HLGKIRFHDLQGDKYYNLGFAYCHSKL 222

Query: 217 ANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIP 276
           ANVL T +LA +L+     VT   VHPG+V++ L   R  F+  L++ + S    LKT  
Sbjct: 223 ANVLFTRELAKRLK--GTGVTTYAVHPGIVRSKL--VRHSFLLCLLWRLFSP--FLKTTW 282

Query: 277 EGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSAS 322
           EGA TS + A    +E ++G YF+DC  +     + ++   AE LW+ S
Sbjct: 283 EGAQTSLHCALAEGLEPLSGKYFSDCK-KTWVSPRARNNKTAERLWNVS 307

BLAST of Carg19489 vs. TrEMBL
Match: tr|A0A1S3BLF4|A0A1S3BLF4_CUCME (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 4.9e-155
Identity = 284/340 (83.53%), Postives = 309/340 (90.88%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLEM KYLVGSAGASGYGS STAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSE +CFD+SRITVM LDLSS+NSV+NF    E+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISQ----KNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTV 240
           IHSWFSGDIFEYL QIS+     NR YD+TRAY+FSKLAN+LHTVQLAH+LQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNY 300
           NCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVAT+R VENVNG Y
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           F DCN+QGSCE  TKSAHLAE LWSASE I+S LTG PQP
Sbjct: 301 FEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 340

BLAST of Carg19489 vs. TrEMBL
Match: tr|A0A1R3IB47|A0A1R3IB47_COCAP (Short-chain dehydrogenase/reductase SDR OS=Corchorus capsularis OX=210143 GN=CCACVL1_13385 PE=3 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 4.8e-118
Identity = 228/329 (69.30%), Postives = 271/329 (82.37%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLE  KYL+GS GASG+GSKSTAE+V+E+   + SITAI+TGATSGIG ETARVLAKRG 
Sbjct: 1   MLETVKYLIGSRGASGFGSKSTAEQVTETSPDLRSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNL 120
           R+++PARNLKAAEEAKARIVSE   F  S I VMALDLSS++SV  F    ESLNLPLNL
Sbjct: 61  RLVLPARNLKAAEEAKARIVSE---FPGSEIVVMALDLSSLSSVRQFVAEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAG+F +EHA SEDGIEMTFATNYLGHFLLTKLLLN MI+TAK TGVQGRIVNV+S 
Sbjct: 121 LINNAGKFAHEHAISEDGIEMTFATNYLGHFLLTKLLLNNMIKTAKETGVQGRIVNVSSM 180

Query: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           +H WFSGD+  YLGQIS+  R YD+TRAYA SKLANVLHT +LA +L+++ ANVTVNCVH
Sbjct: 181 VHGWFSGDMIRYLGQISRSKRSYDATRAYALSKLANVLHTKELAQRLKQVDANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PG+V+T L REREG +TDL+F++ASK  LLKTIP+ AAT+CYVAT+  + NV+G YFADC
Sbjct: 241 PGIVRTRLTREREGLITDLVFFLASK--LLKTIPQAAATTCYVATHPRLVNVSGKYFADC 300

Query: 301 NDQGSCECKTKSAHLAEGLWSASETIVST 328
           N+  + +  + S + A  LW+ASE +VST
Sbjct: 301 NEAWTSKLGSNS-NEASRLWAASEIMVST 323

BLAST of Carg19489 vs. TrEMBL
Match: tr|A0A1Q3BL03|A0A1Q3BL03_CEPFO (Adh_short domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_12200 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.4e-117
Identity = 223/329 (67.78%), Postives = 274/329 (83.28%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           M+E  KYL+GS GASGYGSKSTAE+V+ +C  ++SITAI+TGATSGIG ETARVLAKRG 
Sbjct: 1   MVETVKYLIGSVGASGYGSKSTAEQVTGNCPDLSSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNL 120
           R+++PAR+LKAAEEAKARIVSE   F  S I V+ALDLSS+NSV  F    ESLNLPLNL
Sbjct: 61  RLVLPARSLKAAEEAKARIVSE---FPDSHIIVLALDLSSLNSVRLFVAEFESLNLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAG+F +EHA SEDGIEMTFATNYLGHFLLTK+L+N+M ETAK TG+QGRIVNV+S 
Sbjct: 121 LINNAGKFAHEHAISEDGIEMTFATNYLGHFLLTKMLVNKMSETAKETGIQGRIVNVSSG 180

Query: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           IH+WFSGD+ +YL +I++ N  YD+TRAYA SKLANVLHT +LAH+L+EM+ANVTVNCVH
Sbjct: 181 IHTWFSGDMIQYLAEITRNNCHYDATRAYALSKLANVLHTKELAHRLKEMEANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PG+V+T L REREG +TDL+F++ASK  L+KTIP+ AAT+CYVAT+  + NV+G YF+DC
Sbjct: 241 PGIVRTRLTREREGLITDLLFFLASK--LVKTIPQAAATTCYVATHPRLLNVSGKYFSDC 300

Query: 301 NDQGSCECKTKSAHLAEGLWSASETIVST 328
           N+  + +    S+  A  LWSASE +VS+
Sbjct: 301 NEAWTSKLGANSSEAAR-LWSASEIMVSS 323

BLAST of Carg19489 vs. TrEMBL
Match: tr|A0A0D2PB19|A0A0D2PB19_GOSRA (Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_004G131000 PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 3.1e-117
Identity = 223/328 (67.99%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLE  KYL+GS G SG+GSKSTAEEV+E+C  + SITAI+TGATSGIG ETARVLAKRG 
Sbjct: 1   MLETLKYLIGSTGVSGFGSKSTAEEVTETCPDLRSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNL 120
           R+++PARNLKAAEEAKARIVSE   F  S I VMALDLSS+ SV NF    ESLNLP+N+
Sbjct: 61  RLVLPARNLKAAEEAKARIVSE---FPESEIIVMALDLSSLTSVRNFVSELESLNLPVNI 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAG+F +EHA SEDGIEMTFATNYLGHFLLTKLLLN M++TAK TGV GRIVNV+S+
Sbjct: 121 LINNAGKFAHEHAISEDGIEMTFATNYLGHFLLTKLLLNTMMKTAKQTGVPGRIVNVSSS 180

Query: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           IH WFSGD+  Y+GQIS+    YD+TRAYA SKLANVLHT +LA +L+++ ANVTVNCVH
Sbjct: 181 IHGWFSGDMIRYIGQISRSKSQYDATRAYALSKLANVLHTKELAQRLKQVDANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PG+V+T L REREG +TDL+F++ASK  LLKTIP+ AAT+CYVAT+  + N++G YFADC
Sbjct: 241 PGIVRTRLTREREGLITDLVFFLASK--LLKTIPQAAATTCYVATHPRLLNISGKYFADC 300

Query: 301 NDQGSCECKTKSAHLAEGLWSASETIVS 327
           N+  + +  + S   A+ LW+ASE +VS
Sbjct: 301 NEAWTSKLGSNSNEAAK-LWAASEIMVS 322

BLAST of Carg19489 vs. TrEMBL
Match: tr|A0A2P5WX93|A0A2P5WX93_GOSBA (Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA24946 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 5.3e-117
Identity = 227/330 (68.79%), Postives = 270/330 (81.82%), Query Frame = 0

Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLE  KYL+GS GASG+GSKSTAEEV+E+C  + SITAI+TGATSGIG ETARVLAKRG 
Sbjct: 1   MLETVKYLIGSRGASGFGSKSTAEEVTETCPDLRSITAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFXXXXESLNLPLNL 120
           R++IPARNLKAA EAKARIVSE   F +S I +MALDLSS++SV  F    ESL+LPLNL
Sbjct: 61  RLVIPARNLKAAAEAKARIVSE---FPNSEIVIMALDLSSLSSVRKFVLDFESLDLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAGRF + HA SEDGIEMTFATNYLGHFLLTKLLLN+M+ETAK +GVQGRIVNV+S+
Sbjct: 121 LINNAGRFAHHHAISEDGIEMTFATNYLGHFLLTKLLLNKMMETAKQSGVQGRIVNVSSS 180

Query: 181 IHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 240
           I+ WFSGD+  YLGQIS+    YD+T AYA SKLANVLHT +LA +L+EM ANVTVNCVH
Sbjct: 181 INGWFSGDMIRYLGQISRSKSQYDATLAYALSKLANVLHTKELAQRLKEMGANVTVNCVH 240

Query: 241 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 300
           PG+V+T L REREGF+TDL F +ASK  LLKTIP+ A+T+CYVAT+  +ENV+G YFADC
Sbjct: 241 PGIVRTRLTREREGFITDLFFILASK--LLKTIPQAASTTCYVATHPRLENVSGKYFADC 300

Query: 301 NDQGSCECKTKSAHLAEGLWSASETIVSTL 329
           N+  + +  + S   A  LW+ASE +VS +
Sbjct: 301 NETWTSKLGSDSIE-ASKLWTASEIMVSEI 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022923251.15.5e-182100.00short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch... [more]
XP_023552712.13.6e-18199.40short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ... [more]
XP_022923250.11.4e-18099.70short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
XP_023552711.18.8e-18099.10short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ... [more]
XP_022965266.11.1e-17797.01short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
AT4G24050.12.2e-11666.97NAD(P)-binding Rossmann-fold superfamily protein[more]
AT1G64590.19.5e-11262.95NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G50130.14.5e-7747.68NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G02540.19.0e-7045.32NAD(P)-binding Rossmann-fold superfamily protein[more]
AT2G37540.13.9e-6544.04NAD(P)-binding Rossmann-fold superfamily protein[more]
Match NameE-valueIdentityDescription
sp|A2RVM0|TIC32_ARATH1.1e-5943.60Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
sp|Q6RVV4|TIC32_PEA8.7e-5442.72Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
sp|Q9HBH5|RDH14_HUMAN6.7e-3838.06Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1[more]
sp|Q9ERI6|RDH14_MOUSE8.7e-3838.64Retinol dehydrogenase 14 OS=Mus musculus OX=10090 GN=Rdh14 PE=1 SV=1[more]
sp|P59837|RDH12_BOVIN1.4e-3537.37Retinol dehydrogenase 12 OS=Bos taurus OX=9913 GN=RDH12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BLF4|A0A1S3BLF4_CUCME4.9e-15583.53short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=... [more]
tr|A0A1R3IB47|A0A1R3IB47_COCAP4.8e-11869.30Short-chain dehydrogenase/reductase SDR OS=Corchorus capsularis OX=210143 GN=CCA... [more]
tr|A0A1Q3BL03|A0A1Q3BL03_CEPFO1.4e-11767.78Adh_short domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v... [more]
tr|A0A0D2PB19|A0A0D2PB19_GOSRA3.1e-11767.99Uncharacterized protein OS=Gossypium raimondii OX=29730 GN=B456_004G131000 PE=3 ... [more]
tr|A0A2P5WX93|A0A2P5WX93_GOSBA5.3e-11768.79Uncharacterized protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA24946 PE=3 SV... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR036291NAD(P)-bd_dom_sf
IPR002347SDR_fam
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19489-RACarg19489-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 164..180
score: 27.08
coord: 230..247
score: 36.77
coord: 35..52
score: 46.16
coord: 114..125
score: 42.55
coord: 207..226
score: 30.23
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 35..179
e-value: 4.2E-25
score: 88.3
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 29..328
e-value: 7.2E-71
score: 240.6
NoneNo IPR availablePANTHERPTHR24320FAMILY NOT NAMEDcoord: 6..329
NoneNo IPR availablePANTHERPTHR24320:SF114SUBFAMILY NOT NAMEDcoord: 6..329
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 35..299