Carg16308 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg16308
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	RING/U-box superfamily protein
Location	Cucurbita_argyrosperma_scaffold_088 : 38581 .. 39288 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATCAAATCTCTCGATACGAAAACCTGGTGGTGGAAGTACTTACAGATCTTCCGACGCCGTCACGAATTCCAAAACATCCGTCGCGAATCGAACACATCGGCCTAATCTTCACCCTCCGTCGCCGTCGCAGCCACTTCGAGGTATCGGAGAACGGCGAACGACGACTAATCCAGCAGCGAGAAACCCTAAAAATTCCACTCTGCGACAATCAATTCCGCGCCGAAATCTTCAATCGAGACGACGATTCTCTGTTCCACAGCCTCTCTTGCAGTTTCCAGAACTTCAAACCAGAAATCGACGCGGAACCGGTAGCCGATGCCCTAATCGAGCGGTGGATTGAAATTAGGGACGAAGCGAATTCGAGAAGCTCGGACAGAGTGTTTCCGATGACGGTGAATTTGACGATGTGGCACATTGTGGCTCGGTATGAAATCGGCGTCGGTAGCGGTGGAGACGGCTGCGGAATGGTGCCGGCGTGCGGCGGCGCGGTTGCGAAGATGTTGAGGAGATTGAAGAGGGAAGATGAAGTGGATGGAATTTGTGTGATCTGTTTGGATGAATTGAAGAACAAGAAGAAGAAGAATGGAAGATTTGGTTTACAAATGCCTTGTTTACATGTGTTTCATGGGAATTGCATTGAAGATTGGTTGAAGAATAGCCATTATTGTCCAATTTGTAGATTTGAAGTGCCAATCCAATAG

mRNA sequence

ATGGAACATCAAATCTCTCGATACGAAAACCTGGTGGTGGAAGTACTTACAGATCTTCCGACGCCGTCACGAATTCCAAAACATCCGTCGCGAATCGAACACATCGGCCTAATCTTCACCCTCCGTCGCCGTCGCAGCCACTTCGAGGTATCGGAGAACGGCGAACGACGACTAATCCAGCAGCGAGAAACCCTAAAAATTCCACTCTGCGACAATCAATTCCGCGCCGAAATCTTCAATCGAGACGACGATTCTCTGTTCCACAGCCTCTCTTGCAGTTTCCAGAACTTCAAACCAGAAATCGACGCGGAACCGGTAGCCGATGCCCTAATCGAGCGGTGGATTGAAATTAGGGACGAAGCGAATTCGAGAAGCTCGGACAGAGTGTTTCCGATGACGGTGAATTTGACGATGTGGCACATTGTGGCTCGGTATGAAATCGGCGTCGGTAGCGGTGGAGACGGCTGCGGAATGGTGCCGGCGTGCGGCGGCGCGGTTGCGAAGATGTTGAGGAGATTGAAGAGGGAAGATGAAGTGGATGGAATTTGTGTGATCTGTTTGGATGAATTGAAGAACAAGAAGAAGAAGAATGGAAGATTTGGTTTACAAATGCCTTGTTTACATGTGTTTCATGGGAATTGCATTGAAGATTGGTTGAAGAATAGCCATTATTGTCCAATTTGTAGATTTGAAGTGCCAATCCAATAG

Coding sequence (CDS)

ATGGAACATCAAATCTCTCGATACGAAAACCTGGTGGTGGAAGTACTTACAGATCTTCCGACGCCGTCACGAATTCCAAAACATCCGTCGCGAATCGAACACATCGGCCTAATCTTCACCCTCCGTCGCCGTCGCAGCCACTTCGAGGTATCGGAGAACGGCGAACGACGACTAATCCAGCAGCGAGAAACCCTAAAAATTCCACTCTGCGACAATCAATTCCGCGCCGAAATCTTCAATCGAGACGACGATTCTCTGTTCCACAGCCTCTCTTGCAGTTTCCAGAACTTCAAACCAGAAATCGACGCGGAACCGGTAGCCGATGCCCTAATCGAGCGGTGGATTGAAATTAGGGACGAAGCGAATTCGAGAAGCTCGGACAGAGTGTTTCCGATGACGGTGAATTTGACGATGTGGCACATTGTGGCTCGGTATGAAATCGGCGTCGGTAGCGGTGGAGACGGCTGCGGAATGGTGCCGGCGTGCGGCGGCGCGGTTGCGAAGATGTTGAGGAGATTGAAGAGGGAAGATGAAGTGGATGGAATTTGTGTGATCTGTTTGGATGAATTGAAGAACAAGAAGAAGAAGAATGGAAGATTTGGTTTACAAATGCCTTGTTTACATGTGTTTCATGGGAATTGCATTGAAGATTGGTTGAAGAATAGCCATTATTGTCCAATTTGTAGATTTGAAGTGCCAATCCAATAG

Protein sequence

MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ

Homology

[Close]

BLAST of Carg16308 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata])

HSP 1 Score: 495.0 bits (1273), Expect = 1.4e-136
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD 180
           ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD 180

Query: 181 GICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 236
           GICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ
Sbjct: 181 GICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of Carg16308 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488.4 bits (1256), Expect = 1.3e-134
Identity = 232/235 (98.72%), Postives = 234/235 (99.57%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           MEHQISRYE+LVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ
Sbjct: 1   MEHQISRYESLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD 180
           ANSRSSDRVFPMTVNLT+WHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD 180

Query: 181 GICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 236
           GICVICLDELKNKKKKNGRF LQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ
Sbjct: 181 GICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of Carg16308 vs. NCBI nr
Match: XP_022972455.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima])

HSP 1 Score: 424.1 bits (1089), Expect = 3.1e-115
Identity = 205/235 (87.23%), Postives = 207/235 (88.09%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLR RRSHFEVSE+GERRLIQ
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           QRETLKIPLCDNQFRAEIFNRDDDSLFH+LS SFQNFKPEIDAEPVADALIERWIEIRDE
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVD 180
           ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAV KMLRR        
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVGKMLRRXXXXXXXX 180

Query: 181 GICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 236
                            GRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ
Sbjct: 181 XXXXXXXXXXXXXXXXXGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of Carg16308 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo])

HSP 1 Score: 298.1 bits (762), Expect = 2.6e-77
Identity = 147/237 (62.03%), Postives = 183/237 (77.22%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           ME+QIS+Y    VE+  DLP   RI KHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           ++E  K+PLCD+QF  EIFN DD+SLF  L CSF+N++P+IDAE VA ALIE+WI IRD+
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180
            NSRS  +VF MTVNLT+  +V +Y IG  GS  GG+    VPAC  A+ KML+RL+  D
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           E + IC+ICLDE+  +KKKNGR GL+MPCLHVFHG CIE+WLKNSH CPICRF++PI
Sbjct: 181 EDERICIICLDEV--RKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPI 235

[Close]

BLAST of Carg16308 vs. NCBI nr
Match: XP_011654386.1 (PREDICTED: RING finger protein 141-like [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_4G419590 [Cucumis sativus])

HSP 1 Score: 295.0 bits (754), Expect = 2.2e-76
Identity = 144/237 (60.76%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of Carg16308 vs. TAIR10
Match: AT1G14200.1 (RING/U-box superfamily protein)

HSP 1 Score: 68.6 bits (166), Expect = 6.0e-12
Identity = 34/87 (39.08%), Postives = 44/87 (50.57%), Query Frame = 0


Query: 151 SGGDGCGMVPACGGAVAKMLRRLKRED--EVDGICVICLDELKNKKKKNGRFGLQMPCLH 210
           S G   G  PA    V  M R +  ED  +  G C ICLDE        G    +MPC H
Sbjct: 75  SDGGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEW-----SKGDVAAEMPCKH 134

Query: 211 VFHGNCIEDWLKNSHYCPICRFEVPIQ 236
            FH  C+E+WL     CP+CR+E+P++
Sbjct: 135 KFHSKCVEEWLGRHATCPMCRYEMPVE 156

[Close]

BLAST of Carg16308 vs. TAIR10
Match: AT1G26800.1 (RING/U-box superfamily protein)

HSP 1 Score: 65.9 bits (159), Expect = 3.9e-11
Identity = 27/58 (46.55%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 177 DEVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           D  +G CVICL+E K+++        +MPC H FHG CIE WL     CP+CR+E+P+
Sbjct: 107 DGCEGECVICLEEWKSEETVK-----EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPV 159

[Close]

BLAST of Carg16308 vs. TAIR10
Match: AT3G15740.1 (RING/U-box superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-10
Identity = 34/77 (44.16%), Postives = 43/77 (55.84%), Query Frame = 0


Query: 158 MVPACGGAVAKMLRRLKREDEVDGICVICLDELK-NKKKKNGRFGLQMPCLHVFHGNCIE 217
           + PA   AV   L  L  E+E  G CVIC+D ++     + GR    MPC HVFH  C E
Sbjct: 153 VAPANDEAVEMHLETLVVENE--GYCVICMDNIRVGSDVEAGR----MPCSHVFHRTCGE 212

Query: 218 DWLKNSHYCPICRFEVP 234
           +WL+NS  CP+CR   P
Sbjct: 213 EWLRNSGICPVCRALFP 223

[Close]

BLAST of Carg16308 vs. TAIR10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein)

HSP 1 Score: 61.2 bits (147), Expect = 9.6e-10
Identity = 31/87 (35.63%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 150 GSGGDGCGMVPACGGAVAKMLRRLKRED---EVDGICVICLDELKNKKKKNGRFGLQMPC 209
           G+G    G  PA   A+ + L R++  D   + +  C +C +       + G  G +MPC
Sbjct: 180 GNGIGRSGNPPASKSAI-ESLPRVEISDCHTKAEANCAVCTEVF-----EAGIEGREMPC 239

Query: 210 LHVFHGNCIEDWLKNSHYCPICRFEVP 234
            H+FHG+CI  WL   + CP+CRFE+P
Sbjct: 240 KHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of Carg16308 vs. TAIR10
Match: AT3G14970.1 (RING/U-box superfamily protein)

HSP 1 Score: 61.2 bits (147), Expect = 9.6e-10
Identity = 49/190 (25.79%), Postives = 82/190 (43.16%), Query Frame = 0


Query: 49  EVSENGERRLIQQRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVAD 108
           EV+ +GE       ETL +       +  +F+ D + +   + C   +         V  
Sbjct: 45  EVNSDGEDENPLDLETLLVDRTHEFDKGLLFSGDREQI-QVIVCHILDLIKAPRYIDVVT 104

Query: 109 ALIERWIEIRDEANSRSSDRVFPMTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAK 168
            LI+  ++++ + +  S+ +   + +N+ +W      +             PA   AV +
Sbjct: 105 ELIDAILDLK-KCDKISNVKEVDVVINVIVWRFP---DXXXXXXXXXXXXXPASNEAVEQ 164

Query: 169 MLRRLKREDEVDGICVICLDELKNKKKKNGRFGL-----QMPCLHVFHGNCIEDWLKNSH 228
            L  +  E+E    CVIC+D++        R GL     +MPC HVFH  C EDWL+ S 
Sbjct: 165 HLETVVVENE--SWCVICMDKI--------RIGLDVAAGRMPCSHVFHRTCGEDWLRCSG 219

Query: 229 YCPICRFEVP 234
            CP+CR   P
Sbjct: 225 ICPVCRAMFP 219

[Close]

BLAST of Carg16308 vs. Swiss-Prot
Match: sp|Q9LQX2|MPSR1_ARATH (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.1e-10
Identity = 27/58 (46.55%), Postives = 37/58 (63.79%), Query Frame = 0


Query: 177 DEVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           D  +G CVICL+E K+++        +MPC H FHG CIE WL     CP+CR+E+P+
Sbjct: 107 DGCEGECVICLEEWKSEETVK-----EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPV 159

[Close]

BLAST of Carg16308 vs. Swiss-Prot
Match: sp|Q08109|HRD1_YEAST (ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=HRD1 PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.6e-09
Identity = 35/110 (31.82%), Postives = 52/110 (47.27%), Query Frame = 0


Query: 132 MTVNLTMWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVDGICVICLDEL- 191
           M +   +W I+A Y+ G                 V   + +L+     D IC+IC+DEL 
Sbjct: 298 MLLKDVVWDILALYQSGTSLWKIWRNNKQLDDTLVTVTVEQLQNSANDDNICIICMDELI 357

Query: 192 --------KNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEV 233
                   KNK KK  R    +PC H+ H +C+++W++ S  CPICR  V
Sbjct: 358 HSPNQQTWKNKNKKPKR----LPCGHILHLSCLKNWMERSQTCPICRLPV 403

[Close]

BLAST of Carg16308 vs. Swiss-Prot
Match: sp|Q3T0W3|RN181_BOVIN (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.0e-09
Identity = 27/74 (36.49%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 160 PACGGAVAKMLRRLKREDEVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWL 219
           PA   AV  + R + R  + +  C +CL E + ++       ++MPC H+FH NCI  WL
Sbjct: 53  PAAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEET-----AIEMPCHHLFHSNCILPWL 112

Query: 220 KNSHYCPICRFEVP 234
             ++ CP+CR E+P
Sbjct: 113 SKTNSCPLCRHELP 121

[Close]

BLAST of Carg16308 vs. Swiss-Prot
Match: sp|Q6AXU4|RN181_RAT (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-08
Identity = 26/74 (35.14%), Postives = 41/74 (55.41%), Query Frame = 0


Query: 160 PACGGAVAKMLRRLKREDEVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWL 219
           PA    V  + R + R  + +  C +CL E + ++       ++MPC H+FH NCI  WL
Sbjct: 65  PAAKAVVESLPRTVIRSSKAELKCPVCLLEFEEEETV-----IEMPCHHLFHSNCILPWL 124

Query: 220 KNSHYCPICRFEVP 234
             ++ CP+CR E+P
Sbjct: 125 SKTNSCPLCRHELP 133

[Close]

BLAST of Carg16308 vs. Swiss-Prot
Match: sp|Q9SNB6|RDUF1_ARATH (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 1.7e-08
Identity = 31/87 (35.63%), Postives = 47/87 (54.02%), Query Frame = 0


Query: 150 GSGGDGCGMVPACGGAVAKMLRRLKRED---EVDGICVICLDELKNKKKKNGRFGLQMPC 209
           G+G    G  PA   A+ + L R++  D   + +  C +C +       + G  G +MPC
Sbjct: 180 GNGIGRSGNPPASKSAI-ESLPRVEISDCHTKAEANCAVCTEVF-----EAGIEGREMPC 239

Query: 210 LHVFHGNCIEDWLKNSHYCPICRFEVP 234
            H+FHG+CI  WL   + CP+CRFE+P
Sbjct: 240 KHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of Carg16308 vs. TrEMBL
Match: tr|A0A1S4E0Q9|A0A1S4E0Q9_CUCME (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-77
Identity = 147/237 (62.03%), Postives = 183/237 (77.22%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           ME+QIS+Y    VE+  DLP   RI KHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           ++E  K+PLCD+QF  EIFN DD+SLF  L CSF+N++P+IDAE VA ALIE+WI IRD+
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180
            NSRS  +VF MTVNLT+  +V +Y IG  GS  GG+    VPAC  A+ KML+RL+  D
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           E + IC+ICLDE+  +KKKNGR GL+MPCLHVFHG CIE+WLKNSH CPICRF++PI
Sbjct: 181 EDERICIICLDEV--RKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPI 235

[Close]

BLAST of Carg16308 vs. TrEMBL
Match: tr|A0A0A0L236|A0A0A0L236_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.4e-76
Identity = 144/237 (60.76%), Postives = 182/237 (76.79%), Query Frame = 0


Query: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EDERVCVICLDEV--GKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235

[Close]

BLAST of Carg16308 vs. TrEMBL
Match: tr|E5GCQ4|E5GCQ4_CUCME (Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-26
Identity = 76/221 (34.39%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 26  PKHPSRIEHIGLIFTLRRRRSHFEVSENGE---RRLIQQRETLKIPLCDNQFRAEIFNRD 85
           P  P+ ++ I L F L+ +  HF   E G      L+ +    +      Q    IF R 
Sbjct: 40  PNSPALVKQIDLNFKLQVKCQHFGAGEEGSTFATTLLSEHIISQPTFPALQLPISIFKRG 99

Query: 86  DDSLFHSLSCSFQNFKPEIDAEPVADALIERWI---EIRDEANSRSSDRVFPMTVNLTMW 145
           D +L   L   FQ F+  ++ E V D +I+ WI   E   E NSR    ++P+ + + + 
Sbjct: 100 DKTLKRLLFQKFQMFRGIVNVELVVDEIIKHWIRKVEEDQENNSRVFAEIYPLEIRIEL- 159

Query: 146 HIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEV-----DGI-CVICLDELKNK 205
            +V R    +        MVP    A+  ML+R++  +E+     D I CVICL+++  +
Sbjct: 160 -LVCRMIPAIIDQPQEVMMVPTSDNAMESMLKRVENNEELMKLGDDSIDCVICLEKIGKE 219

Query: 206 KKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
           +K++GR  LQMPCLH+FH  CI  WLK SH+CP CRF +PI
Sbjct: 220 EKRSGRVVLQMPCLHMFHEECIRKWLKTSHFCPTCRFSMPI 258

[Close]

BLAST of Carg16308 vs. TrEMBL
Match: tr|A0A0A0K7L0|A0A0A0K7L0_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 6.4e-24
Identity = 75/227 (33.04%), Postives = 114/227 (50.22%), Query Frame = 0


Query: 26  PKHPSRIEHIGLIFTLRRRRSH-FEVSENGE----RRLIQQRETLKIPLCDNQFRAEIFN 85
           P  P+ ++ I L F +  +  H F+  E G       L+ +  +   PL   Q    IF 
Sbjct: 40  PNSPTLVKQIDLNFKVEVKCQHYFQAGEGGNSTFATALLSEHISSHHPLPTLQLPVSIFK 99

Query: 86  RDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDEANSRSS--DRVFPM--TVNL 145
             D +L   L   FQ ++  I+ E V D +I+ W++  DE NS S   + ++P+  T+ L
Sbjct: 100 HGDKTLKRLLFREFQIYRAIINIELVVDEIIKHWVKKVDEENSTSQVFEEIYPLEITIEL 159

Query: 146 TMWHIV----ARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKRE------DEVDGICVIC 205
            ++ ++      +EI +        MVP    A+  ML++++ E      D     CVIC
Sbjct: 160 LVYQMIHAIDQPHEIMMDQ--PQVMMVPTSESAMESMLKKVENEEIVKFGDNKSINCVIC 219

Query: 206 LDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVP 234
           L+E+  +KK +    LQMPCLH+FH  CI  WLK SHYCP CRF +P
Sbjct: 220 LEEISKEKKGSEAVVLQMPCLHMFHEECIRKWLKRSHYCPTCRFSMP 264

[Close]

BLAST of Carg16308 vs. TrEMBL
Match: tr|A0A0A0K493|A0A0A0K493_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.4e-23
Identity = 71/223 (31.84%), Postives = 111/223 (49.78%), Query Frame = 0


Query: 29  PSRIEHIGLIFTLRRRRSHFEVSENGE---RRLIQQRETLKIPLCDNQFRAEIFNRDDDS 88
           P+ ++ I L   +  +  HF+  E G+     L+ +      P    Q    +F   + +
Sbjct: 42  PTFVKQINLKLKVEVKCQHFQADEEGDTFASTLMIEHIVSHHPFPTFQLPVSVFRHGNRT 101

Query: 89  LFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDEANSRSS---DRVFPMTVN-----LT 148
           L   L   FQ F+  I+ + VAD +IE W++  ++  + S    + ++P+ +      L 
Sbjct: 102 LKRLLFQQFQIFRGIINIQLVADEIIEHWVKKEEDRENNSGVLLEEIYPLEITIELLLLR 161

Query: 149 MWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEV------DGICVICLDELK 208
           M H + + E  +        MVPA   A+  ML+R+++E+ V         CVICL+E+ 
Sbjct: 162 MIHAIDQPEETIDQ--PQVTMVPASESAMESMLKRVEKEEIVKVGEDKSINCVICLEEIS 221

Query: 209 NKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 235
            K K +    LQMPCLH+FH  CI  WLK SHYCP CRF +PI
Sbjct: 222 KKMKGSEGVVLQMPCLHMFHEECIRTWLKTSHYCPTCRFSMPI 262

The following BLAST results are available for this feature:

BLAST of Carg16308 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952293.1	1.4e-136	100.00	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata]	[more]
XP_023511497.1	1.3e-134	98.72	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]
XP_022972455.1	3.1e-115	87.23	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita maxima]	[more]
XP_016901804.1	2.6e-77	62.03	PREDICTED: RING finger protein 141-like [Cucumis melo]	[more]
XP_011654386.1	2.2e-76	60.76	PREDICTED: RING finger protein 141-like [Cucumis sativus] >KGN54667.1 hypothetic...	[more]

BLAST of Carg16308 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G14200.1	6.0e-12	39.08	RING/U-box superfamily protein	[more]
AT1G26800.1	3.9e-11	46.55	RING/U-box superfamily protein	[more]
AT3G15740.1	1.9e-10	44.16	RING/U-box superfamily protein	[more]
AT3G46620.1	9.6e-10	35.63	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G14970.1	9.6e-10	25.79	RING/U-box superfamily protein	[more]

BLAST of Carg16308 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9LQX2\|MPSR1_ARATH	7.1e-10	46.55	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
sp\|Q08109\|HRD1_YEAST	1.6e-09	31.82	ERAD-associated E3 ubiquitin-protein ligase HRD1 OS=Saccharomyces cerevisiae (st...	[more]
sp\|Q3T0W3\|RN181_BOVIN	6.0e-09	36.49	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
sp\|Q6AXU4\|RN181_RAT	1.3e-08	35.14	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
sp\|Q9SNB6\|RDUF1_ARATH	1.7e-08	35.63	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]

BLAST of Carg16308 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4E0Q9\|A0A1S4E0Q9_CUCME	1.7e-77	62.03	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]
tr\|A0A0A0L236\|A0A0A0L236_CUCSA	1.4e-76	60.76	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1	[more]
tr\|E5GCQ4\|E5GCQ4_CUCME	1.1e-26	34.39	Hypothetical zinc finger protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
tr\|A0A0A0K7L0\|A0A0A0K7L0_CUCSA	6.4e-24	33.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067560 PE=4 SV=1	[more]
tr\|A0A0A0K493\|A0A0A0K493_CUCSA	2.4e-23	31.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G067540 PE=4 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013083	Znf_RING/FYVE/PHD
IPR001841	Znf_RING

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16308-RA	Carg16308-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 183..228 e-value: 2.5E-7 score: 40.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 183..229 e-value: 7.3E-12 score: 45.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 183..229 score: 12.607
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 164..233 e-value: 2.1E-16 score: 60.9
None	No IPR available	PANTHER	PTHR45491	FAMILY NOT NAMED	coord: 158..234
None	No IPR available	SUPERFAMILY	SSF57850	RING/U-box	coord: 177..234

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg16308	Csa7G067540	Cucumber (Chinese Long) v2	carcuB0487
Carg16308	MELO3C018566	Melon (DHL92) v3.5.1	carmeB0460
Carg16308	CSPI07G06330	Wild cucumber (PI 183967)	carcpiB0493
Carg16308	Cucsa.218660	Cucumber (Gy14) v1	carcgyB0524
Carg16308	Cp4.1LG15g04030	Cucurbita pepo (Zucchini)	carcpeB0637
Carg16308	CsGy7G005960	Cucumber (Gy14) v2	carcgybB0464
Carg16308	MELO3C028580.2	Melon (DHL92) v3.6.1	carmedB0463
Carg16308	CsaV3_7G007310	Cucumber (Chinese Long) v3	carcucB0490

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg16308	Bottle gourd (USVL1VR-Ls)	carlsiB412
Carg16308	Watermelon (Charleston Gray)	carwcgB0445
Carg16308	Watermelon (97103) v2	carwmbB0477
Carg16308	Wax gourd	carwgoB0547
Carg16308	Silver-seed gourd	carcarB402
Carg16308	Silver-seed gourd	carcarB456
Carg16308	Cucurbita maxima (Rimu)	carcmaB0630
Carg16308	Cucurbita maxima (Rimu)	carcmaB0632
Carg16308	Cucurbita maxima (Rimu)	carcmaB0634
Carg16308	Cucurbita moschata (Rifu)	carcmoB0612
Carg16308	Cucurbita moschata (Rifu)	carcmoB0615
Carg16308	Cucurbita moschata (Rifu)	carcmoB0616
Carg16308	Cucurbita pepo (Zucchini)	carcpeB0639