Carg14195 (gene) Silver-seed gourd

Overview
NameCarg14195
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionDNA-directed RNA polymerases I, II, and III subunit rpabc3
LocationCucurbita_argyrosperma_scaffold_045 : 528228 .. 530051 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTTCATGAAGCTTGTAACTGCTATCTCATTCTTTTAATTCACATTTTATGATCTTTTAACTGTCTATGAGTCCTTTTGTAGACAGTAACTTCTGCACCATCTTGACTTTTATGTGAATGATTTGATATGGTGTTTATTGGGTGCATAAAATTGTTCTTAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAGTAATATTTCTCCCCTCAATGTTTAATTGAATTTCATACAATCTTGGTGCTATCTGGTAGTCGTTTCATGTCTCTTCATTGTTTAAAAGAACACTCGGAAACACCTTGCTGCTAGATATCTGCATCCATATGTACCTTATAGATTCTAAAAGAAACGACCAATGATTTCATAATCTTTCTCTGATGCTGAAATTTTGGAGAACGGATTTTACCTAGCGATCTATAATAGCCCAAGCTCACTGCTAGCAGACATTGTCCTCTTCGAGTTTTTCCTTTCGGGCTTCCAAACATGTCTCCTAGGGAGAGGTTTCCACACCCTTATAAGGAATGTTTTGTTCTCCTCTCCAACTGATGTGGAGTCTCACAATCCATCCCCCTTCGAGGCCCGATGTCCTTGCTGGCACTCAATCAACTCTCCAATCGATGTAGGATCTCACAATTCACCTCTCTTCGAGGTCCAACATCCTCGCTGCCACACTGTCTGATGTCCACCCCCTTCGGGGCTTAGTGTCCTCACTGGCACACCGCCCGGTGTCCATCCCCCTTGGGACTCAGTGTTCTCGCTGGCACACCATCCGGTATCTGCCTTTGACACCATTTGTAATGGTCCAGACCATCAATAGCAAATATTGTCCTCCTTGGGCTTTTCCTTTTGGACTTCTCCTCAAATTTTTAAAATCCATCTACTAAAGAGAGGTTTTCACGCCCTTATAAGAAATGCTCTGTTCCCCTCTACAATCGACGTGGGATCTCACAGTTTCTATAAACTTTTCCTAAAGTAAAAAACAGAACAGTGATTATAAGCAAACAGCTTTATGTTCTAAAAACAAAATTATGCAAATGTTTCTTAGTATGGGAAGTGCCGAGTAGGCACTAATATCAAGCACAAACCCGTAACTTCAGCCGTGAAGATTGTGAACATTGTGAACATAATTGAAATCTGTAGTAGCAGGAGTAATATTTTTGAACTAAACTTAAGTTGTTGCAGAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTGTAAGTGAATTGCCATGATTTTGCTCACCCATTTCTGCTGCTGATTTTCCTAAAAGTCTAAGTGGAAGTACTTTATGCATGATTCTAACTCGATTCATAAAACATATTTTGATGTCGGAATTGGGTGTATTTTACAGAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGAACCTCAACAATCTCTCCCAATTATCAATTATAATTCTAGATAACTTATGAATTGATTGAAGGCTTTCTTTAATTTGGAAGCTTGTGTGAAGC

mRNA sequence

ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGAACCTCAACAATCTCTCCCAATTATCAATTATAATTCTAGATAACTTATGAATTGATTGAAGGCTTTCTTTAATTTGGAAGCTTGTGTGAAGC

Coding sequence (CDS)

ATGGCGGAGCTTCTATTTGATGATATTTTCAAGGTCAAGTGCGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCTAGAATTGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGACAAGATTCTGATGGTCCTGTCTCCTACGCTGAATTATGATGGAGCTCCTGTTACCGGCTATAGTGGTCAAAAAGGGAAGAAATCACTAGCAGATAAGTTCGAGTATATCATGCATGGGAAGCTGTACAAACTCGCAGATGAAGGCTCAGGATCAGACTTGAAAGTAGAGGTTTATGCATCTTTTGGAGGACTTCAAATGATGTTGAAGGGAGATCCCCTCCACTGTGCCAAATTCAGGGTAGACCAGAACATGTTCTTGCTCCTCCGAAAGCTCACATGA

Protein sequence

MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLKGDPLHCAKFRVDQNMFLLLRKLT
Homology
[Close]
BLAST of Carg14195 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 293.1 bits (749), Expect = 5.2e-76
Identity = 143/146 (97.95%), Postives = 145/146 (99.32%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAE+LFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 147
           MLKGDPLHCAKFRVDQNMFLLLRKLT
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Carg14195 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 287.3 bits (734), Expect = 2.8e-74
Identity = 141/145 (97.24%), Postives = 142/145 (97.93%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMH KLYKLADEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 146
           MLKGDPLHC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]
BLAST of Carg14195 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284.3 bits (726), Expect = 2.4e-73
Identity = 136/145 (93.79%), Postives = 144/145 (99.31%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Carg14195 vs. NCBI nr
Match: XP_004149143.1 (PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucumis sativus] >XP_011653128.1 PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucumis sativus] >XP_011653129.1 PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucumis sativus] >KGN53231.1 hypothetical protein Csa_4G031020 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 1.5e-70
Identity = 132/146 (90.41%), Postives = 141/146 (96.58%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA LLF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVT Y  Q+GKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 147
           +LKGDP+HCAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Carg14195 vs. NCBI nr
Match: XP_022136431.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Momordica charantia])

HSP 1 Score: 272.7 bits (696), Expect = 7.2e-70
Identity = 130/145 (89.66%), Postives = 141/145 (97.24%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           M ELLFDDIFKVKCVNPDG+KYDKVSRI+ARSEKL+M+MLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MVELLFDDIFKVKCVNPDGQKYDKVSRIDARSEKLAMHMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPV  +S Q+GKKSLADKFEY+MHGKLYKL++EGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVASHSSQEGKKSLADKFEYVMHGKLYKLSEEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 146
           MLKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPVHCAKFRVDQNMFLLLRKL 145

[Close]
BLAST of Carg14195 vs. TAIR10
Match: AT3G59600.1 (RNA polymerase Rpb8)

HSP 1 Score: 177.6 bits (449), Expect = 5.7e-45
Identity = 83/142 (58.45%), Postives = 108/142 (76.06%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  GDK  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Carg14195 vs. TAIR10
Match: AT1G54250.1 (RNA polymerase Rpb8)

HSP 1 Score: 177.2 bits (448), Expect = 7.5e-45
Identity = 84/142 (59.15%), Postives = 107/142 (75.35%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GDK  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Carg14195 vs. Swiss-Prot
Match: sp|Q9M1A8|RPB8B_ARATH (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.0e-43
Identity = 83/142 (58.45%), Postives = 108/142 (76.06%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           ++F+DIF V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  GDK  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+L+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Carg14195 vs. Swiss-Prot
Match: sp|O81097|RPB8A_ARATH (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.3e-43
Identity = 84/142 (59.15%), Postives = 107/142 (75.35%), Query Frame = 0 

Query: 4   LLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVLSPT 63
           +LF+DIF V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  GDK  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQMMLK 123
           LN DG P TGY     KK+LADK+EYIMHGKLYK++ E  G   K E+Y SFGGL M+LK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPLHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]
BLAST of Carg14195 vs. Swiss-Prot
Match: sp|Q19826|RPAB3_CAEEL (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.4e-32
Identity = 65/145 (44.83%), Postives = 101/145 (69.66%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA ++FDD+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + DK+ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG    G    K +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Carg14195 vs. Swiss-Prot
Match: sp|A8XT81|RPAB3_CAEBR (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.5e-31
Identity = 64/145 (44.14%), Postives = 99/145 (68.28%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA ++FDD+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + DK  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG    G    K +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRVDQNMFLLLRK 145
           M LKG+ ++   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]
BLAST of Carg14195 vs. Swiss-Prot
Match: sp|P52434|RPAB3_HUMAN (DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=9606 GN=POLR2H PE=1 SV=4)

HSP 1 Score: 132.1 bits (331), Expect = 5.0e-30
Identity = 64/148 (43.24%), Postives = 100/148 (67.57%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA +LF+DIF VK ++P+GKK+D+VSR+   SE   M ++LDVN +IYP+D GDK  +V+
Sbjct: 1   MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI 60

Query: 61  SPTLNYDGAPVTG-YSGQKGKKSLADKFEYIMHGKLYKLADEGSGSD--LKVEVYASFGG 120
           + TL  DG    G Y+    + S AD+FEY+M+GK+Y++  + + ++   ++  Y S+GG
Sbjct: 61  ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGG 120

Query: 121 LQMMLKGDPLHCAKFRVDQNMFLLLRKL 146
           L M L+GD  +   F VD  ++LL++KL
Sbjct: 121 LLMRLQGDANNLHGFEVDSRVYLLMKKL 148

[Close]
BLAST of Carg14195 vs. TrEMBL
Match: tr|A0A0A0KZC2|A0A0A0KZC2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 9.6e-71
Identity = 132/146 (90.41%), Postives = 141/146 (96.58%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA LLF+DIFKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVT Y  Q+GKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 147
           +LKGDP+HCAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Carg14195 vs. TrEMBL
Match: tr|A0A1S3BY97|A0A1S3BY97_CUCME (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494404 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.3e-69
Identity = 129/146 (88.36%), Postives = 140/146 (95.89%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           MA LLF+D+FKVKCVNPDGKKYDKVSRI+ARSEKLSM MLLDVNTEIYPIDEG+K+LMVL
Sbjct: 1   MAGLLFNDVFKVKCVNPDGKKYDKVSRIDARSEKLSMSMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVT Y  Q+GKKSLADKFEYIMHGK YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKLT 147
           +LKGDP++CAKFRVDQNMFLLLRKLT
Sbjct: 121 LLKGDPINCAKFRVDQNMFLLLRKLT 146

[Close]
BLAST of Carg14195 vs. TrEMBL
Match: tr|A0A200PX79|A0A200PX79_9MAGN (RNA polymerase OS=Macleaya cordata OX=56857 GN=BVC80_9095g35 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.0e-51
Identity = 99/145 (68.28%), Postives = 121/145 (83.45%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           M ELLF+DIF +  ++PDGKK+DKVSRIEARSE+  MYM LDVNTEIYP+  GDK  MVL
Sbjct: 1   MVELLFEDIFTLIRLDPDGKKFDKVSRIEARSEQFDMYMQLDVNTEIYPLSVGDKFTMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           +PTL+ DG P TGY  Q G+KSLADKFEY+MHGKLYK+++EGSG ++KVE+YASFGGL M
Sbjct: 61  APTLSLDGTPDTGYFMQGGRKSLADKFEYVMHGKLYKISEEGSGINVKVEIYASFGGLLM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 146
           MLKGDP + A F +DQ +FLL+RK+
Sbjct: 121 MLKGDPSNAANFELDQRLFLLMRKV 145

[Close]
BLAST of Carg14195 vs. TrEMBL
Match: tr|A0A200QQI5|A0A200QQI5_9MAGN (RNA polymerase OS=Macleaya cordata OX=56857 GN=BVC80_9019g32 PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.5e-51
Identity = 98/145 (67.59%), Postives = 121/145 (83.45%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           M ELLF+DIF +  ++PDGKK+DKVSRIEA+SE+  MYM LDVNTEIYP+  GDK  MVL
Sbjct: 1   MVELLFEDIFTLIRLDPDGKKFDKVSRIEAKSEQFDMYMQLDVNTEIYPLSVGDKFTMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120
           +PTL+ DG P TGY  Q G+KSLADKFEY+MHGKLYK+++EGSG ++KVE+YASFGGL M
Sbjct: 61  APTLSLDGTPDTGYFTQGGRKSLADKFEYVMHGKLYKISEEGSGINVKVEIYASFGGLLM 120

Query: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 146
           MLKGDP + A F +DQ +FLL+RK+
Sbjct: 121 MLKGDPSNAANFELDQRLFLLMRKV 145

[Close]
BLAST of Carg14195 vs. TrEMBL
Match: tr|M0U2U0|M0U2U0_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103998751 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 2.1e-49
Identity = 97/146 (66.44%), Postives = 122/146 (83.56%), Query Frame = 0 

Query: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60
           M ELLF+DIF +  ++PDGKK+DKVSRIEARSE+  MYM LDVNTEIYP++ GDK  MVL
Sbjct: 1   MVELLFEDIFTLTRLDPDGKKFDKVSRIEARSEQFDMYMQLDVNTEIYPLNVGDKFTMVL 60

Query: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGS-DLKVEVYASFGGLQ 120
           +PTL+ DG P +GY  Q G+KSLADKFEY+MHGKLYK+++E SG  ++KVE+YASFGGL 
Sbjct: 61  TPTLSLDGTPDSGYFTQAGRKSLADKFEYVMHGKLYKISEEASGGPNVKVEIYASFGGLL 120

Query: 121 MMLKGDPLHCAKFRVDQNMFLLLRKL 146
           MMLKGDP + A+F +DQ +FLL+RK+
Sbjct: 121 MMLKGDPSNAAQFELDQRLFLLMRKV 146

The following BLAST results are available for this feature:
BLAST of Carg14195 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022983196.15.2e-7697.95DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP... [more]
XP_022942165.12.8e-7497.24DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata][more]
XP_022929200.12.4e-7393.79DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >... [more]
XP_004149143.11.5e-7090.41PREDICTED: DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucumis sa... [more]
XP_022136431.17.2e-7089.66DNA-directed RNA polymerases II and V subunit 8A-like [Momordica charantia][more]
BLAST of Carg14195 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G59600.15.7e-4558.45RNA polymerase Rpb8[more]
AT1G54250.17.5e-4559.15RNA polymerase Rpb8[more]
BLAST of Carg14195 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9M1A8|RPB8B_ARATH1.0e-4358.45DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
sp|O81097|RPB8A_ARATH1.3e-4359.15DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
sp|Q19826|RPAB3_CAEEL1.4e-3244.83Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
sp|A8XT81|RPAB3_CAEBR1.5e-3144.14Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
sp|P52434|RPAB3_HUMAN5.0e-3043.24DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Homo sapiens OX=96... [more]
BLAST of Carg14195 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0KZC2|A0A0A0KZC2_CUCSA9.6e-7190.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1[more]
tr|A0A1S3BY97|A0A1S3BY97_CUCME5.3e-6988.36DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
tr|A0A200PX79|A0A200PX79_9MAGN5.0e-5168.28RNA polymerase OS=Macleaya cordata OX=56857 GN=BVC80_9095g35 PE=3 SV=1[more]
tr|A0A200QQI5|A0A200QQI5_9MAGN8.5e-5167.59RNA polymerase OS=Macleaya cordata OX=56857 GN=BVC80_9019g32 PE=3 SV=1[more]
tr|M0U2U0|M0U2U0_MUSAM2.1e-4966.44Uncharacterized protein OS=Musa acuminata subsp. malaccensis OX=214687 GN=103998... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0006351transcription, DNA-templated
Vocabulary: INTERPRO
TermDefinition
IPR012340NA-bd_OB-fold
IPR005570RNA_pol_Rpb8
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14195-RACarg14195-RAmRNA


InterPro
Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..144
e-value: 5.0E-59
score: 212.1
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..146
e-value: 2.4E-48
score: 161.8
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..144
e-value: 5.8E-49
score: 165.8
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..145
NoneNo IPR availableGENE3DG3DSA:2.40.50.140coord: 6..146
e-value: 7.3E-50
score: 170.5
NoneNo IPR availablePANTHERPTHR10917:SF1SUBFAMILY NOT NAMEDcoord: 1..145
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILYSSF50249Nucleic acid-binding proteinscoord: 3..144

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg14195Csa4G031020Cucumber (Chinese Long) v2carcuB0958
Carg14195MELO3C017579Melon (DHL92) v3.5.1carmeB0924
Carg14195CSPI04G04750Wild cucumber (PI 183967)carcpiB0981
Carg14195Cucsa.029450Cucumber (Gy14) v1carcgyB1056
Carg14195CmaCh16G006790Cucurbita maxima (Rimu)carcmaB1286
Carg14195CmaCh04G007950Cucurbita maxima (Rimu)carcmaB1288
Carg14195CmoCh04G008550Cucurbita moschata (Rifu)carcmoB1259
Carg14195CmoCh16G007370Cucurbita moschata (Rifu)carcmoB1258
Carg14195Cp4.1LG14g01400Cucurbita pepo (Zucchini)carcpeB1301
Carg14195Cp4.1LG01g09630Cucurbita pepo (Zucchini)carcpeB1302
Carg14195CsGy4G004680Cucumber (Gy14) v2carcgybB0931
Carg14195MELO3C017579.2Melon (DHL92) v3.6.1carmedB0930
Carg14195CsaV3_UNG188480Cucumber (Chinese Long) v3carcucB0986
Carg14195CsaV3_4G004830Cucumber (Chinese Long) v3carcucB0982
Carg14195Bhi01G001922Wax gourdcarwgoB1086
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg14195Carg05790Silver-seed gourdcarcarB646
The following block(s) are covering this gene:
GeneOrganismBlock
Carg14195Silver-seed gourdcarcarB602
Carg14195Cucurbita maxima (Rimu)carcmaB1287
Carg14195Cucurbita maxima (Rimu)carcmaB1289
Carg14195Cucurbita pepo (Zucchini)carcpeB1300
Carg14195Cucurbita pepo (Zucchini)carcpeB1304
Carg14195Wild cucumber (PI 183967)carcpiB0982
Carg14195Bottle gourd (USVL1VR-Ls)carlsiB859
Carg14195Watermelon (Charleston Gray)carwcgB0915
Carg14195Watermelon (Charleston Gray)carwcgB0916
Carg14195Watermelon (97103) v1carwmB0940
Carg14195Watermelon (97103) v1carwmB0942
Carg14195Watermelon (97103) v2carwmbB0987
Carg14195Watermelon (97103) v2carwmbB0989