Carg09262 (gene) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg09262
Type	gene
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	cytochrome b5
Location	Cucurbita_argyrosperma_scaffold_018 : 275155 .. 276821 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCGAAGCCGAACCACCCGCAGCGGCGATTAGTCGACAATTGTCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTACGTCCCTGATAGTATTCATGGCCTTACTTAATTTTCTTTCACAAATTTTTGTTCCGTTCGTCGGCCTTACAGTATTCTCTTCATGTATGTGCTTCGCCATATGATTCTCATGGAATTTTTTTCTGAGTTTTTTTTTTTTTTTTTTTCGATTTGTTATTCCACGAGATGCGTTCTAAATTATGGTCGGAATTTGTTTAAGGCGCTCTCGAGTTGTGGACTGAATTCAATTTTTGTGGTTGATTGTCGTCTTTTTGGCTGGGGGATGGGAAGTTGATGCTATAGTTTTACTGGAATTAGCTGAACTTTGTCACTGTGATGGTGAGAAATGAAGTAAACAATCTTATCATGTTCAGATTTTTTTATTTCTGATATTCCATTATACAGTGTTCATAGTTGTAATTGATGAGGATCGAGACTCTTTAAGTTTAAATGAAGTTCTGTTTAGAAGTAACTGTCTGATTAATATAGGCATGAATTTATAAACCATTTTAGGAGTTGAGATCTGATGAAATCTCTTGGCCTTAATTTCTCCTATCCACCTGAAGTTTCTTCTTCTACTATCTAACTTTGTTTTGTTCCCACTCTTGGAGGCAGAGTGGAGATAACCGGATTAAAGTAGAAGGTAAAAGAATGGTGGATGGATGGATGGATGGAAACTCACCAACATGGTTCACTCTTAAACCAATAGATTTATTTTGATTTCTAGTTCATCGTCATGTCAAATTCTTAGAATTAACAATACAAAAGTAAGGGATTCCAATACTGAATTCAAATAGGACTCGAAGTTTCAATATCCTCTTAGAACGTATGTTACTGTTTGAATCTGATACTTTATTGGATTGGATGTGTCAATTCTGACAAATTGTTGATTCAAAATTCTTGAGAGGGAAATTGACGTTTTAGTTGCGTGTTTGAAACATCGGCCCTCCATTTCCTCGCTCACCATCTTTGCCAAAATATTGATACTTTCGTTTTTACTTCGGTCCACGATCAATTTTCTGTCCACACATTCTCCATTGACACCATTCTACAACATGTCCACCTCCTCTGACCAGTAAAAATGTGTCATCTTTTACCATATTTTGTTTGAGAGGATGCGTTCTGACATTACTTCAAAATGACATGTTATCGTTCATAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGGTATGCTTGTACTTTGTAATGGATTCTTCTCTCACAAATCTGAACACTGCACTTTTTTGTCCCATCAAAGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

mRNA sequence

TCCGAAGCCGAACCACCCGCAGCGGCGATTAGTCGACAATTGTCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGGTATGCTTGTACTTTGTAATGGATTCTTCTCTCACAAATCTGAACACTGCACTTTTTTGTCCCATCAAAGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

Coding sequence (CDS)

ATGCCGAGCATTTCGGCCCTCTATTCCATAGAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTGTATGATTTGACGTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGGTATGCTTGTACTTTGTAATGGATTCTTCTCTCACAAATCTGAACACTGCACTTTTTTGTCCCATCAAAGGCAGAGATGCCACAGATGATTTCGAAGATGCCGGTCATAGCAAAGATGCTCGAGAGCTGATGGAGAAGTTCTATATCGGCGAGCTTGATCCTTCTTCCTCAGCGATCCCAGAACTCGAAACAAAAGAACCAGATGGTTATGCAGCTCGGGTTCAGACCTTGACAAAGCAATACTGGGGGGTTCCTATTGCAGTACTTGGTATTTCTGTAGTACTTGGCTGGTTATACTTGCTCAAAAAGTGA

Protein sequence

MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK

Homology

[Close]

BLAST of Carg09262 vs. NCBI nr
Match: XP_022961879.1 (cytochrome b5 [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 8.3e-64
Identity = 129/156 (82.69%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT         
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELE KEP
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELEAKEP 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           DGYAARVQTLTKQYWG+PIAVLGISVVLGWLYLLKK
Sbjct: 121 DGYAARVQTLTKQYWGIPIAVLGISVVLGWLYLLKK 131

[Close]

BLAST of Carg09262 vs. NCBI nr
Match: XP_023545611.1 (cytochrome b5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-63
Identity = 129/156 (82.69%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT         
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           DGYAARVQTLTKQYWGVPIAVLG+SV LGWLYLLKK
Sbjct: 121 DGYAARVQTLTKQYWGVPIAVLGMSVALGWLYLLKK 131

[Close]

BLAST of Carg09262 vs. NCBI nr
Match: XP_022997294.1 (cytochrome b5 [Cucurbita maxima])

HSP 1 Score: 245.4 bits (625), Expect = 1.3e-61
Identity = 123/156 (78.85%), Postives = 129/156 (82.69%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSIEE+S+HSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVI+AAT         
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKD RELM+KFYIGELDPSSSAIP LETKEP
Sbjct: 61  ----------------GRDATDDFEDAGHSKDTRELMKKFYIGELDPSSSAIPALETKEP 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           DGYAA+VQTLTKQYWG+PIAVLGISVVLGWLYLLKK
Sbjct: 121 DGYAAQVQTLTKQYWGIPIAVLGISVVLGWLYLLKK 131

[Close]

BLAST of Carg09262 vs. NCBI nr
Match: XP_004144166.1 (PREDICTED: cytochrome b5 isoform A [Cucumis sativus] >KGN47656.1 hypothetical protein Csa_6G366540 [Cucumis sativus])

HSP 1 Score: 217.2 bits (552), Expect = 3.8e-53
Identity = 113/156 (72.44%), Postives = 118/156 (75.64%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT         
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELMEKFYIG LD SS    +LET + 
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQV 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           DGYA RVQTLTKQYW  P+AV+GISVVLGW YL KK
Sbjct: 121 DGYATRVQTLTKQYWKAPVAVIGISVVLGWAYLRKK 131

[Close]

BLAST of Carg09262 vs. NCBI nr
Match: XP_022131367.1 (cytochrome b5 [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 8.6e-53
Identity = 108/156 (69.23%), Postives = 120/156 (76.92%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSIS LYSI+E+S HSSNDDCWI+IDGKVYDLTSYLDEHPGGDDVI+AAT         
Sbjct: 1   MPSISNLYSIQEISHHSSNDDCWIVIDGKVYDLTSYLDEHPGGDDVILAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELM+KFYIGELD SSSAIPEL+T++ 
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMQKFYIGELDASSSAIPELKTEQL 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           D YAARVQ L KQYW  P+A+LGISVV+GWL+L  K
Sbjct: 121 DDYAARVQGLAKQYWAAPVAMLGISVVIGWLFLRNK 131

[Close]

BLAST of Carg09262 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 154.5 bits (389), Expect = 5.6e-38
Identity = 78/159 (49.06%), Postives = 105/159 (66.04%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MP+++ LYS+EE + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A           
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLA----------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSS-SAIPEL---E 120
                         + G+DATDDFEDAGHSKDARELMEK++IGELD SS   IPEL   +
Sbjct: 61  --------------VAGKDATDDFEDAGHSKDARELMEKYFIGELDESSLPEIPELKIYK 120

Query: 121 TKEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLK 156
             +P     ++  LTKQYW VP++++ ISV +  L+  K
Sbjct: 121 KDQPQDSVQKLFDLTKQYWVVPVSIITISVAVSVLFSRK 134

[Close]

BLAST of Carg09262 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 112.1 bits (279), Expect = 3.2e-25
Identity = 56/146 (38.36%), Postives = 90/146 (61.64%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTN 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++T               
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSST--------------- 66

Query: 67  LNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAAR 126
                     G+DATDDFED GHS+ ARE+ME++Y+GE+DP++  IP+     P      
Sbjct: 67  ----------GKDATDDFEDVGHSESAREMMEQYYVGEIDPTT--IPKKVKYTPPKQPHY 125

Query: 127 VQTLTKQY------WGVPIAVLGISV 147
            Q  T ++      + VP+A+LG++V
Sbjct: 127 NQDKTSEFIIKLLQFLVPLAILGLAV 125

[Close]

BLAST of Carg09262 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 109.0 bits (271), Expect = 2.7e-24
Identity = 59/154 (38.31%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           M S   + S EEVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++T         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSST--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           G+DAT+DFED GHS  AR++M+K++IGE+D  SS++P   T   
Sbjct: 61  ----------------GKDATNDFEDVGHSDTARDMMDKYFIGEID--SSSVPATRTYVA 120

Query: 121 DGYAARVQTLTKQY------WGVPIAVLGISVVL 149
               A  Q  T ++      + VPI +LG+++V+
Sbjct: 121 PQQPAYNQDKTPEFIIKILQFLVPILILGLALVV 127

[Close]

BLAST of Carg09262 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 105.1 bits (261), Expect = 3.9e-23
Identity = 55/151 (36.42%), Postives = 84/151 (55.63%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTN 66
           ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +T               
Sbjct: 7   VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTST--------------- 66

Query: 67  LNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELD------------PSSSAIPE 126
                     G+DATDDFED GHS  A+ +++++Y+G++D            P+S+    
Sbjct: 67  ----------GKDATDDFEDVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVA 126

Query: 127 LETKEPDGYAARVQTLTKQYWGVPIAVLGIS 146
            + K  D     +Q L      VP+ +LG++
Sbjct: 127 TQDKSSDFVIKLLQFL------VPLLILGLA 126

[Close]

BLAST of Carg09262 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 97.4 bits (241), Expect = 8.1e-21
Identity = 52/149 (34.90%), Postives = 84/149 (56.38%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTN 66
           L S  +V++H   +DCWI+I GKVYD+++++DEHPGGD+V++A T               
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVT--------------- 63

Query: 67  LNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAAR 126
                     G+DA+ DFED  HSKDA+ELM+K+ IG++D S+  + +      +  +  
Sbjct: 64  ----------GKDASIDFEDVNHSKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTA 123

Query: 127 VQTLTKQYWG-------VPIAVLGISVVL 149
            +T TK+  G       +P+ +LG++  L
Sbjct: 124 AET-TKEESGKKLLIYLIPLLILGVAFAL 126

[Close]

BLAST of Carg09262 vs. Swiss-Prot
Match: sp|Q9FDW8|CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.0e-36
Identity = 78/159 (49.06%), Postives = 105/159 (66.04%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MP+++ LYS+EE + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A           
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLA----------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSS-SAIPEL---E 120
                         + G+DATDDFEDAGHSKDARELMEK++IGELD SS   IPEL   +
Sbjct: 61  --------------VAGKDATDDFEDAGHSKDARELMEKYFIGELDESSLPEIPELKIYK 120

Query: 121 TKEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLK 156
             +P     ++  LTKQYW VP++++ ISV +  L+  K
Sbjct: 121 KDQPQDSVQKLFDLTKQYWVVPVSIITISVAVSVLFSRK 134

[Close]

BLAST of Carg09262 vs. Swiss-Prot
Match: sp|O48845|CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.7e-24
Identity = 56/146 (38.36%), Postives = 90/146 (61.64%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTN 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++T               
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSST--------------- 66

Query: 67  LNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAAR 126
                     G+DATDDFED GHS+ ARE+ME++Y+GE+DP++  IP+     P      
Sbjct: 67  ----------GKDATDDFEDVGHSESAREMMEQYYVGEIDPTT--IPKKVKYTPPKQPHY 125

Query: 127 VQTLTKQY------WGVPIAVLGISV 147
            Q  T ++      + VP+A+LG++V
Sbjct: 127 NQDKTSEFIIKLLQFLVPLAILGLAV 125

[Close]

BLAST of Carg09262 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 9.7e-24
Identity = 61/154 (39.61%), Postives = 88/154 (57.14%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           M S   +   EEVSQH+   DCW+II GKVYD+T ++D+HPGGD+V++++T         
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSST--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           G+DAT+DFED GHS  AR++MEK+YIGE+D  SS +P   T   
Sbjct: 61  ----------------GKDATNDFEDVGHSDTARDMMEKYYIGEID--SSTVPATRTYVA 120

Query: 121 DGYAARVQTLTKQY------WGVPIAVLGISVVL 149
               A  Q  T ++      + VPI +LG+++V+
Sbjct: 121 PVQPAYNQDKTPEFMIKILQFLVPILILGLALVV 127

[Close]

BLAST of Carg09262 vs. Swiss-Prot
Match: sp|Q42342|CYB5E_ARATH (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 4.8e-23
Identity = 59/154 (38.31%), Postives = 90/154 (58.44%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           M S   + S EEVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++T         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSST--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           G+DAT+DFED GHS  AR++M+K++IGE+D  SS++P   T   
Sbjct: 61  ----------------GKDATNDFEDVGHSDTARDMMDKYFIGEID--SSSVPATRTYVA 120

Query: 121 DGYAARVQTLTKQY------WGVPIAVLGISVVL 149
               A  Q  T ++      + VPI +LG+++V+
Sbjct: 121 PQQPAYNQDKTPEFIIKILQFLVPILILGLALVV 127

[Close]

BLAST of Carg09262 vs. Swiss-Prot
Match: sp|P49100|CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-22
Identity = 55/148 (37.16%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 7   LYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVMDSSLTN 66
           +Y++EEV++H+S DDCW+II GKVY+++ +L++HPGGDDV++++T               
Sbjct: 8   VYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSST--------------- 67

Query: 67  LNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAAR 126
                     G+DATDDFED GHS  AR +M+++Y+G++D  +S IP      P      
Sbjct: 68  ----------GKDATDDFEDVGHSTTARAMMDEYYVGDID--TSTIPARTKYVPPKQPHY 127

Query: 127 VQTLTKQY------WGVPIAVLGISVVL 149
            Q  T ++      + VP+A+LG++V +
Sbjct: 128 NQDKTPEFIIKILQFLVPLAILGLAVAI 128

[Close]

BLAST of Carg09262 vs. TrEMBL
Match: tr|A0A0A0KIR1|A0A0A0KIR1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.5e-53
Identity = 113/156 (72.44%), Postives = 118/156 (75.64%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT         
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELMEKFYIG LD SS    +LET + 
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQV 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           DGYA RVQTLTKQYW  P+AV+GISVVLGW YL KK
Sbjct: 121 DGYATRVQTLTKQYWKAPVAVIGISVVLGWAYLRKK 131

[Close]

BLAST of Carg09262 vs. TrEMBL
Match: tr|A0A1S3BCR4|A0A1S3BCR4_CUCME (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.8e-52
Identity = 109/156 (69.87%), Postives = 118/156 (75.64%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MPSISALYSI+EVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDD+IV AT         
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELETKEP 120
                           GRDATDDFEDAGHSKDARELMEKFYIG LD SSS   +LET + 
Sbjct: 61  ----------------GRDATDDFEDAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQV 120

Query: 121 DGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           D YA RVQTLTKQYW  P+AV+GIS+VLGW+YL KK
Sbjct: 121 DSYATRVQTLTKQYWKAPVAVIGISMVLGWVYLRKK 131

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BLAST of Carg09262 vs. TrEMBL
Match: tr|A0A1U8MC43|A0A1U8MC43_GOSHI (cytochrome b5-like OS=Gossypium hirsutum OX=3635 GN=LOC107936222 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.6e-42
Identity = 88/159 (55.35%), Postives = 111/159 (69.81%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MP+++ LY++EE SQH++ DDCW++IDGKVYD+T YLDEHPGGDDV++AAT         
Sbjct: 1   MPTLTKLYTMEEASQHNTKDDCWVVIDGKVYDVTPYLDEHPGGDDVVLAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELE---T 120
                           G+DATDDFEDAGHSK A+ELM+ FYIGELD S+  IPELE    
Sbjct: 61  ----------------GKDATDDFEDAGHSKSAKELMQNFYIGELDTSAPIIPELEIASK 120

Query: 121 KEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           KE   Y+  +  LTKQYW VP+A++GISVV G++YL KK
Sbjct: 121 KETANYSRNLMDLTKQYWAVPVAIVGISVVAGFIYLRKK 134

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BLAST of Carg09262 vs. TrEMBL
Match: tr|A0A061E9I3|A0A061E9I3_THECC (Cytochrome B5, n6,ATCB5-A,CB5-A OS=Theobroma cacao OX=3641 GN=TCM_011490 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-41
Identity = 88/159 (55.35%), Postives = 113/159 (71.07%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MP+++ LY+++E SQH+S +DCW++IDGKVYD+TSYLDEHPGGDDV++A+T         
Sbjct: 1   MPTLTKLYTMQEASQHNSKEDCWVVIDGKVYDVTSYLDEHPGGDDVVLAST--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELE---T 120
                           G+DATDDFEDAGHSK A+ELM+ F IGELD SS  IPELE    
Sbjct: 61  ----------------GKDATDDFEDAGHSKSAKELMQTFCIGELDTSSPIIPELEISSK 120

Query: 121 KEPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           KE   Y+ ++  LTKQYW VP+AV+GISVV+G+LYL +K
Sbjct: 121 KETTDYSQKLMGLTKQYWAVPVAVVGISVVVGFLYLRRK 134

[Close]

BLAST of Carg09262 vs. TrEMBL
Match: tr|A0A2I4H8C1|A0A2I4H8C1_9ROSI (cytochrome b5 OS=Juglans regia OX=51240 GN=LOC109014390 PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.7e-41
Identity = 90/158 (56.96%), Postives = 113/158 (71.52%), Query Frame = 0


Query: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGMVCLYFVM 60
           MP++  LY+++E SQH+S +DCW++IDGKVYD++SYLDEHPGGDDV++AAT         
Sbjct: 1   MPTLWKLYTMQEASQHNSKEDCWVVIDGKVYDVSSYLDEHPGGDDVVLAAT--------- 60

Query: 61  DSSLTNLNTALFCPIKGRDATDDFEDAGHSKDARELMEKFYIGELDPSSSAIPELE--TK 120
                           G+DATDDFEDAGHS+ A+ELME F IGELD SS  IPELE  +K
Sbjct: 61  ----------------GKDATDDFEDAGHSESAKELMETFCIGELDTSSPVIPELEISSK 120

Query: 121 EPDGYAARVQTLTKQYWGVPIAVLGISVVLGWLYLLKK 157
           +   YA ++  LTKQYW VP+AV+GISVVLG+LYL KK
Sbjct: 121 KQTDYAQKLVELTKQYWVVPVAVVGISVVLGFLYLRKK 133

The following BLAST results are available for this feature:

BLAST of Carg09262 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961879.1	8.3e-64	82.69	cytochrome b5 [Cucurbita moschata]	[more]
XP_023545611.1	1.1e-63	82.69	cytochrome b5 [Cucurbita pepo subsp. pepo]	[more]
XP_022997294.1	1.3e-61	78.85	cytochrome b5 [Cucurbita maxima]	[more]
XP_004144166.1	3.8e-53	72.44	PREDICTED: cytochrome b5 isoform A [Cucumis sativus] >KGN47656.1 hypothetical pr...	[more]
XP_022131367.1	8.6e-53	69.23	cytochrome b5 [Momordica charantia]	[more]

BLAST of Carg09262 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G26340.1	5.6e-38	49.06	cytochrome B5 isoform A	[more]
AT2G32720.1	3.2e-25	38.36	cytochrome B5 isoform B	[more]
AT5G53560.1	2.7e-24	38.31	cytochrome B5 isoform E	[more]
AT5G48810.1	3.9e-23	36.42	cytochrome B5 isoform D	[more]
AT2G46650.1	8.1e-21	34.90	cytochrome B5 isoform C	[more]

BLAST of Carg09262 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FDW8\|CYB5A_ARATH	1.0e-36	49.06	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
sp\|O48845\|CYB5B_ARATH	5.7e-24	38.36	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
sp\|P40934\|CYB5_BRAOB	9.7e-24	39.61	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|Q42342\|CYB5E_ARATH	4.8e-23	38.31	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
sp\|P49100\|CYB5_ORYSJ	1.1e-22	37.16	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of Carg09262 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KIR1\|A0A0A0KIR1_CUCSA	2.5e-53	72.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1	[more]
tr\|A0A1S3BCR4\|A0A1S3BCR4_CUCME	4.8e-52	69.87	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1	[more]
tr\|A0A1U8MC43\|A0A1U8MC43_GOSHI	2.6e-42	55.35	cytochrome b5-like OS=Gossypium hirsutum OX=3635 GN=LOC107936222 PE=3 SV=1	[more]
tr\|A0A061E9I3\|A0A061E9I3_THECC	1.3e-41	55.35	Cytochrome B5, n6,ATCB5-A,CB5-A OS=Theobroma cacao OX=3641 GN=TCM_011490 PE=3 SV...	[more]
tr\|A0A2I4H8C1\|A0A2I4H8C1_9ROSI	1.7e-41	56.96	cytochrome b5 OS=Juglans regia OX=51240 GN=LOC109014390 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09262-RA	Carg09262-RA	mRNA

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..106 e-value: 1.4E-25 score: 101.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 10..52 e-value: 3.0E-15 score: 56.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..106 score: 25.225
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 68..117 e-value: 9.1E-9 score: 37.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 3..66 e-value: 1.4E-20 score: 75.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..53
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 75..110
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..53
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 1..53
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 75..156
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 75..156
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Carg09262	Cla022031	Watermelon (97103) v1	carwmB0134
Carg09262	Csa6G366540	Cucumber (Chinese Long) v2	carcuB0158
Carg09262	MELO3C011415	Melon (DHL92) v3.5.1	carmeB0142
Carg09262	ClCG08G010480	Watermelon (Charleston Gray)	carwcgB0138
Carg09262	CSPI06G19730	Wild cucumber (PI 183967)	carcpiB0163
Carg09262	Cucsa.327920	Cucumber (Gy14) v1	carcgyB0175
Carg09262	CmaCh05G004510	Cucurbita maxima (Rimu)	carcmaB0189
Carg09262	CmoCh05G004770	Cucurbita moschata (Rifu)	carcmoB0196
Carg09262	CsGy6G019280	Cucumber (Gy14) v2	carcgybB0156
Carg09262	MELO3C011415.2	Melon (DHL92) v3.6.1	carmedB0146
Carg09262	CsaV3_6G033730	Cucumber (Chinese Long) v3	carcucB0166
Carg09262	Cla97C08G153330	Watermelon (97103) v2	carwmbB0150
Carg09262	Bhi04G000922	Wax gourd	carwgoB0177

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Carg09262	Silver-seed gourd	carcarB164
Carg09262	Cucurbita maxima (Rimu)	carcmaB0185
Carg09262	Cucurbita moschata (Rifu)	carcmoB0194
Carg09262	Cucurbita pepo (Zucchini)	carcpeB0199
Carg09262	Cucurbita pepo (Zucchini)	carcpeB0207
Carg09262	Bottle gourd (USVL1VR-Ls)	carlsiB129