CsGy6G019280 (gene) Cucumber (Gy14) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsGy6G019280
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v2)
Description	cytochrome b5
Location	Chr6 : 19707505 .. 19710550 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAGATGGTTAAGAAAGGAGAGATAGAATGTTAGAACGTGTCGAAGAGCCACACCCTTAGGCCTTAGCGCAGCCGCAGCCGCAGCCGCAGCCAGCAAGAAAGGGACAAAGGAAAGGCGTTCTCACGTTTGAATCCCAATCTTGGCTCTCTCTCTCCCTCCCTCCCCCTCCAACTTTCCTCCCTCTACTTCTTACGCTTTTATTCTAATTCCCCATATCCCATCATTCCCCATCTCATCTCTCTCTTCTTCCTCTCCCATCTTCTTTATTCTTTTTTCCTTTTTCCTTTTATTTTATTATACTCTTTCTATTTAATTTAAAAATTACTAGGCTAAAATTCACATAAATAAAAATATATATGTTTTCTTTTGTTCATCGTGATACTCACATTTTTTGTTGGGCTTTTTGTTTCTTTAGTAAATACTCCTATGGCCCAACGAAGGACTGGATGAGGCCCAAAACCCACCAAGAAGTTCGATATTTCGAGGGCCAGTTCTTCAAAAAAGAGAAGAAAAAGAAAAAAATCCGATCGTGGTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTACGTCCCTGACAATCTTCATAGCCTCCTATAGTTTTATTTTCCTCATCCCTATATGATTCTTATGTAATTTCCTCTCTGGGTTGTTTTCGGTTCATGCTTGTTTTTATTCTACTTGTGATTCTAGAGATGTAAACTGAATTCAATTTTTATTGTTGATGATTGTCTTTTTGGGTTGAAGAGGTGGGGAGTTGAAGCTATAGTTTGACTGGAATTACCTGAATTTGTCAATGTAATGGAGAGAATAGCAGCACACAAGCATATGATGTCCAAGATTCTTTGATTTATGTTGTTGATGGTTTGTAGGTTATCTGGATTTTAGGTAAGTGTTGATTATGATAGTTGTAAATGATAAGGAACATAAGTTAATTCTAAATGAGATCCTATTTAGAATAAAGTAATTATCTGTCAATATAGGTATGAATCATAAACTACTTTAAGAGGTGAAATCTGAGAAAGGCTATTGGCTTGAATTTCTCTTCTCCATCTAAAATATATTCTTCAACACTCTAAGATTCGTGTTGTTCCCACTCTTGGAGGCAGAGTGGAGATAACAGGATTCAGTGTTTTTGTGAATTAGGCCTGGGCTGTTTCATTCTTTTCGAAAAGAATGATTAGGACTTTCCCCTTCTATTAGCTAAGGAAAATAAAATTTACTAAGCCCAAATGTTTTTATTTTCTATAGTCCCACTTCAACTATGCTTTTACATTATTTATGTAGCACAAATTTAAGTTTGCCCCTTAGAAAACCCAAAAAAAAGTTTGCACTTCCTTTTTTCCTCCTTACGCTTGAGCGCTGAAAGACTATTGTTGCACTTTATTAGGCCCCGAGCTTTAAAAAACATTGGCCCAATTAGAGTAGAAGGGAAAAAAGGAAGGATGGATTTGAAAACCCAACCAACAACATGGTTCACATTTGAATCAGTAGATATATTTTAATGCTCAATTCATTGTCATGTCAAACGTTTAGGATTAACAATACTAAAGTAATGGGTTACTGTACTGAATTCAAATAAGACTAAAAGTTTCAATCTCCTCATAGAACTTATGTTAGTGTTTGAATCTGAGAATTTGCTTGAGTGGATATGTCATTTCTTATGATTTGTTGATTTCAAATTCTTGAGAGGTAATTGGCTTTTTCGTGGGGTCTTTGAAACATTGGCCCACCATTTCCAGAATCTTTTTAAAATTTATGTGCTCATTTTCTTTATGGCAGCCTTTCAGTCAAGTCATCTAGTTTGTCAAAAATTGTGAATGTCTTTTCTATGAAAGAATTGTGTTTGAGGATGAGAAGGATGAGTCCTATCACTCATATTTTTCCTAGCTCACCATCTTTGCCACAAAAAACTGATATTTTCATTTTTACTTTATATTTGAGTCCCATGATCAGTTTTCTGTCGACCATTGACACCATTCTACGACACGTCAACCTCTTCTAACCATTTTGTGTGTTTTCTTTTAACGTATCTTCTCCAAGAGGACACACTTTGTCATTGCTTTAAATTGATATATTATCATTGTTAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGATTTTACTTTGTAATGTAAATTTCTCTACCAAATTTGAACAGTTACAAATATAACTTTTTGTCCTATCAAAGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGATTCCTGTGCCAATTAAAGGCTGTTGGCTTGTTGAGTTGAGAGAGTTGGTCTCATTTTTGCTTCTTGAAAAACTTCATATTCGTTTCAATATATTCATACAATGATTGTGGTAGGTTCTTTGTTCCTTAGTAGTACTGTAGAAAGGATTATTTTCTTGTTAATTGGTAGGTTTGTTTATTTGTTTCCATAGTTTCATTAGATGGGAGTTGGGAAAATGGTTTCGGGTTCAATGGAGTTTGATCCAACTCCATTGTCTCTTTTTGACCAAACTATATATACTGTTCTCTACATGCAGCAGTTAATTTTGAGCCTGAAGACATGTGACATCTTATACTTCTACAAGAGAATCAAACAATCCAATGAGACTAAAATAATATCAAAATGCTATATCGAATCAAACTGTTATCATTTGATAACCCTTCTGTTTTTAATTTTGAAATTTATGCTTGGTTTTTTCTCTCAAT

mRNA sequence

CTAAGATGGTTAAGAAAGGAGAGATAGAATGTTAGAACGTGTCGAAGAGCCACACCCTTAGGCCTTAGCGCAGCCGCAGCCGCAGCCGCAGCCAGCAAGAAAGGGACAAAGGAAAGGCGTTCTCACGTTTGAATCCCAATCTTGGCTCTCTCTCTCCCTCCCTCCCCCTCCAACTTTCCTCCCTCTACTTCTTACGCTTTTATTCTAATTCCCCATATCCCATCATTCCCCATCTCATCTCTCTCTTCTTCCTCTCCCATCTTCTTTATTCTTTTTTCCTTTTTCCTTTTATTTTATTATACTCTTTCTATTTAATTTAAAAATTACTAGGCTAAAATTCACATAAATAAAAATATATATGTTTTCTTTTGTTCATCGTGATACTCACATTTTTTGTTGGGCTTTTTGTTTCTTTAGTAAATACTCCTATGGCCCAACGAAGGACTGGATGAGGCCCAAAACCCACCAAGAAGTTCGATATTTCGAGGGCCAGTTCTTCAAAAAAGAGAAGAAAAAGAAAAAAATCCGATCGTGGTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGATTCCTGTGCCAATTAAAGGCTGTTGGCTTGTTGAGTTGAGAGAGTTGGTCTCATTTTTGCTTCTTGAAAAACTTCATATTCGTTTCAATATATTCATACAATGATTGTGGTAGGTTCTTTGTTCCTTAGTAGTACTGTAGAAAGGATTATTTTCTTGTTAATTGGTAGGTTTGTTTATTTGTTTCCATAGTTTCATTAGATGGGAGTTGGGAAAATGGTTTCGGGTTCAATGGAGTTTGATCCAACTCCATTGTCTCTTTTTGACCAAACTATATATACTGTTCTCTACATGCAGCAGTTAATTTTGAGCCTGAAGACATGTGACATCTTATACTTCTACAAGAGAATCAAACAATCCAATGAGACTAAAATAATATCAAAATGCTATATCGAATCAAACTGTTATCATTTGATAACCCTTCTGTTTTTAATTTTGAAATTTATGCTTGGTTTTTTCTCTCAAT

Coding sequence (CDS)

ATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGA

Protein sequence

MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGISVVLGWAYLRKK

Homology

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BLAST of CsGy6G019280 vs. NCBI nr
Match: XP_004144166.1 (PREDICTED: cytochrome b5 isoform A [Cucumis sativus] >KGN47656.1 hypothetical protein Csa_6G366540 [Cucumis sativus])

HSP 1 Score: 266.2 bits (679), Expect = 6.1e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGWAYLRKK
Sbjct: 121 VVLGWAYLRKK 131

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BLAST of CsGy6G019280 vs. NCBI nr
Match: XP_008445442.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 255.0 bits (650), Expect = 1.4e-64
Identity = 124/131 (94.66%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDD+IV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSS DSLKLETNQVD YATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           +VLGW YLRKK
Sbjct: 121 MVLGWVYLRKK 131

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BLAST of CsGy6G019280 vs. NCBI nr
Match: XP_022961879.1 (cytochrome b5 [Cucurbita moschata])

HSP 1 Score: 228.0 bits (580), Expect = 1.8e-56
Identity = 112/131 (85.50%), Postives = 117/131 (89.31%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIG LD SS    +LE  + DGYA RVQTLTKQYW  P+AV+GIS
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELEAKEPDGYAARVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGW YL KK
Sbjct: 121 VVLGWLYLLKK 131

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BLAST of CsGy6G019280 vs. NCBI nr
Match: XP_023545611.1 (cytochrome b5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 227.6 bits (579), Expect = 2.4e-56
Identity = 111/131 (84.73%), Postives = 117/131 (89.31%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSI+EVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIEEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIG LD SS    +LET + DGYA RVQTLTKQYW  P+AV+G+S
Sbjct: 61  DAGHSKDARELMEKFYIGELDPSSSAIPELETKEPDGYAARVQTLTKQYWGVPIAVLGMS 120

Query: 121 VVLGWAYLRKK 132
           V LGW YL KK
Sbjct: 121 VALGWLYLLKK 131

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BLAST of CsGy6G019280 vs. NCBI nr
Match: XP_022997294.1 (cytochrome b5 [Cucurbita maxima])

HSP 1 Score: 222.2 bits (565), Expect = 1.0e-54
Identity = 107/131 (81.68%), Postives = 116/131 (88.55%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSI+E+S+HSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVI+AATGRDATDDFE
Sbjct: 1   MPSISALYSIEEISRHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIIAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKD RELM+KFYIG LD SS     LET + DGYA +VQTLTKQYW  P+AV+GIS
Sbjct: 61  DAGHSKDTRELMKKFYIGELDPSSSAIPALETKEPDGYAAQVQTLTKQYWGIPIAVLGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGW YL KK
Sbjct: 121 VVLGWLYLLKK 131

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BLAST of CsGy6G019280 vs. TAIR10
Match: AT1G26340.1 (cytochrome B5 isoform A)

HSP 1 Score: 160.2 bits (404), Expect = 8.5e-40
Identity = 74/134 (55.22%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLK----LETNQVDGYATRVQTLTKQYWKAPVAV 120
           DAGHSKDARELMEK++IG LD SSL  +      + +Q      ++  LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 IGISVVLGWAYLRK 131
           I ISV +   + RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of CsGy6G019280 vs. TAIR10
Match: AT5G48810.1 (cytochrome B5 isoform D)

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-26
Identity = 46/79 (58.23%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMEKFYIGLLDTSSL 86
           A+ +++++Y+G +DT+++
Sbjct: 67 TAKAMLDEYYVGDIDTATV 85

[Close]

BLAST of CsGy6G019280 vs. TAIR10
Match: AT2G32720.1 (cytochrome B5 isoform B)

HSP 1 Score: 114.8 bits (286), Expect = 4.1e-26
Identity = 49/119 (41.18%), Postives = 86/119 (72.27%), Query Frame = 0


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAVIGISV 122
            ARE+ME++Y+G +D +++      T     +  + +T    +    +  P+A++G++V
Sbjct: 67  SAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAV 125

[Close]

BLAST of CsGy6G019280 vs. TAIR10
Match: AT5G53560.1 (cytochrome B5 isoform E)

HSP 1 Score: 112.5 bits (280), Expect = 2.0e-25
Identity = 46/89 (51.69%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 1  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
          M S   + S +EVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1  MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61 DAGHSKDARELMEKFYIGLLDTSSLDSLK 90
          D GHS  AR++M+K++IG +D+SS+ + +
Sbjct: 61 DVGHSDTARDMMDKYFIGEIDSSSVPATR 89

[Close]

BLAST of CsGy6G019280 vs. TAIR10
Match: AT2G46650.1 (cytochrome B5 isoform C)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-23
Identity = 50/123 (40.65%), Postives = 82/123 (66.67%), Query Frame = 0


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           L S  +V++H   +DCWI+I GKVYD+++++DEHPGGD+V++A TG+DA+ DFED  HSK
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWK------APVAVIGIS 124
           DA+ELM+K+ IG +D S++   +      +  +T  +T  ++  K       P+ ++G++
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

[Close]

BLAST of CsGy6G019280 vs. Swiss-Prot
Match: sp|Q9FDW8|CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.5e-38
Identity = 74/134 (55.22%), Postives = 100/134 (74.63%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLK----LETNQVDGYATRVQTLTKQYWKAPVAV 120
           DAGHSKDARELMEK++IG LD SSL  +      + +Q      ++  LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 IGISVVLGWAYLRK 131
           I ISV +   + RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of CsGy6G019280 vs. Swiss-Prot
Match: sp|Q9ZWT2|CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-25
Identity = 46/79 (58.23%), Postives = 69/79 (87.34%), Query Frame = 0


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMEKFYIGLLDTSSL 86
           A+ +++++Y+G +DT+++
Sbjct: 67 TAKAMLDEYYVGDIDTATV 85

[Close]

BLAST of CsGy6G019280 vs. Swiss-Prot
Match: sp|P40934|CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.3e-25
Identity = 52/127 (40.94%), Postives = 87/127 (68.50%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           M S   +   +EVSQH+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAV 120
           D GHS  AR++MEK+YIG +D+S++ + +     V     + +T    +    +  P+ +
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 IGISVVL 124
           +G+++V+
Sbjct: 121 LGLALVV 127

[Close]

BLAST of CsGy6G019280 vs. Swiss-Prot
Match: sp|O04354|CYB5_BOROF (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 5.7e-25
Identity = 44/81 (54.32%), Postives = 72/81 (88.89%), Query Frame = 0


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EV+QH+++ DCW+II+GKVYD+T +L++HPGGDDV+++ATG+DATDDFED GHS 
Sbjct: 4  IFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHSS 63

Query: 67 DARELMEKFYIGLLDTSSLDS 88
           A+ +++++Y+G +D+SS+ S
Sbjct: 64 SAKAMLDEYYVGDIDSSSIPS 84

[Close]

BLAST of CsGy6G019280 vs. Swiss-Prot
Match: sp|O48845|CYB5B_ARATH (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 7.4e-25
Identity = 49/119 (41.18%), Postives = 86/119 (72.27%), Query Frame = 0


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAVIGISV 122
            ARE+ME++Y+G +D +++      T     +  + +T    +    +  P+A++G++V
Sbjct: 67  SAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAV 125

[Close]

BLAST of CsGy6G019280 vs. TrEMBL
Match: tr|A0A0A0KIR1|A0A0A0KIR1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.0e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGWAYLRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of CsGy6G019280 vs. TrEMBL
Match: tr|A0A1S3BCR4|A0A1S3BCR4_CUCME (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 9.2e-65
Identity = 124/131 (94.66%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDD+IV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSS DSLKLETNQVD YATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           +VLGW YLRKK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of CsGy6G019280 vs. TrEMBL
Match: tr|A0A2P4J2D2|A0A2P4J2D2_QUESU (Cytochrome b5 isoform a OS=Quercus suber OX=58331 GN=CFP56_40832 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 9.0e-44
Identity = 82/131 (62.60%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP++S LYS+QE S+H++ DDCW++IDGKVYD+T+YLDEHPGGDDV++AATG+DAT++FE
Sbjct: 1   MPTLSKLYSMQEASEHNTKDDCWVVIDGKVYDVTTYLDEHPGGDDVVLAATGKDATEEFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSK A+ELME F IG LDT+ +  L++ + +   YA ++  LTKQYW  PVAV+ +S
Sbjct: 61  DAGHSKSAKELMETFCIGELDTTYIPQLEISSKKQTDYAQKLMDLTKQYWVVPVAVVSVS 120

Query: 121 VVLGWAYLRKK 132
           VVLG+ YLRKK
Sbjct: 121 VVLGFLYLRKK 131

[Close]

BLAST of CsGy6G019280 vs. TrEMBL
Match: tr|A0A2I4H8C1|A0A2I4H8C1_9ROSI (cytochrome b5 OS=Juglans regia OX=51240 GN=LOC109014390 PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.6e-43
Identity = 86/133 (64.66%), Postives = 110/133 (82.71%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP++  LY++QE SQH+S +DCW++IDGKVYD++SYLDEHPGGDDV++AATG+DATDDFE
Sbjct: 1   MPTLWKLYTMQEASQHNSKEDCWVVIDGKVYDVSSYLDEHPGGDDVVLAATGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSS--LDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIG 120
           DAGHS+ A+ELME F IG LDTSS  +  L++ + +   YA ++  LTKQYW  PVAV+G
Sbjct: 61  DAGHSESAKELMETFCIGELDTSSPVIPELEISSKKQTDYAQKLVELTKQYWVVPVAVVG 120

Query: 121 ISVVLGWAYLRKK 132
           ISVVLG+ YLRKK
Sbjct: 121 ISVVLGFLYLRKK 133

[Close]

BLAST of CsGy6G019280 vs. TrEMBL
Match: tr|A0A2P6R2E8|A0A2P6R2E8_ROSCH (Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr4g0437951 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 5.8e-43
Identity = 84/131 (64.12%), Postives = 107/131 (81.68%), Query Frame = 0


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY++QE SQH++ DDCWI++DGKVYD+++YLD+HPGGDDVI+A TG+DATDDFE
Sbjct: 1   MPTLTKLYTMQEASQHNTKDDCWIVVDGKVYDVSTYLDDHPGGDDVILATTGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSK A+ELME   IG LDT +  S +LE NQ  GY  ++  LTKQYW  P AV+GIS
Sbjct: 61  DAGHSKSAKELMETMCIGELDTDTSASAELE-NQPTGYPQKLMILTKQYWAVPAAVVGIS 120

Query: 121 VVLGWAYLRKK 132
           VV+G+ YLRKK
Sbjct: 121 VVVGFVYLRKK 130

The following BLAST results are available for this feature:

BLAST of CsGy6G019280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144166.1	6.1e-68	100.00	PREDICTED: cytochrome b5 isoform A [Cucumis sativus] >KGN47656.1 hypothetical pr...	[more]
XP_008445442.1	1.4e-64	94.66	PREDICTED: cytochrome b5 [Cucumis melo]	[more]
XP_022961879.1	1.8e-56	85.50	cytochrome b5 [Cucurbita moschata]	[more]
XP_023545611.1	2.4e-56	84.73	cytochrome b5 [Cucurbita pepo subsp. pepo]	[more]
XP_022997294.1	1.0e-54	81.68	cytochrome b5 [Cucurbita maxima]	[more]

BLAST of CsGy6G019280 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G26340.1	8.5e-40	55.22	cytochrome B5 isoform A	[more]
AT5G48810.1	1.1e-26	58.23	cytochrome B5 isoform D	[more]
AT2G32720.1	4.1e-26	41.18	cytochrome B5 isoform B	[more]
AT5G53560.1	2.0e-25	51.69	cytochrome B5 isoform E	[more]
AT2G46650.1	1.1e-23	40.65	cytochrome B5 isoform C	[more]

BLAST of CsGy6G019280 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9FDW8\|CYB5A_ARATH	1.5e-38	55.22	Cytochrome b5 isoform A OS=Arabidopsis thaliana OX=3702 GN=CB5-A PE=1 SV=1	[more]
sp\|Q9ZWT2\|CYB5D_ARATH	1.9e-25	58.23	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
sp\|P40934\|CYB5_BRAOB	4.3e-25	40.94	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
sp\|O04354\|CYB5_BOROF	5.7e-25	54.32	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]
sp\|O48845\|CYB5B_ARATH	7.4e-25	41.18	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]

BLAST of CsGy6G019280 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KIR1\|A0A0A0KIR1_CUCSA	4.0e-68	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G366540 PE=3 SV=1	[more]
tr\|A0A1S3BCR4\|A0A1S3BCR4_CUCME	9.2e-65	94.66	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103488463 PE=3 SV=1	[more]
tr\|A0A2P4J2D2\|A0A2P4J2D2_QUESU	9.0e-44	62.60	Cytochrome b5 isoform a OS=Quercus suber OX=58331 GN=CFP56_40832 PE=3 SV=1	[more]
tr\|A0A2I4H8C1\|A0A2I4H8C1_9ROSI	2.6e-43	64.66	cytochrome b5 OS=Juglans regia OX=51240 GN=LOC109014390 PE=3 SV=1	[more]
tr\|A0A2P6R2E8\|A0A2P6R2E8_ROSCH	5.8e-43	64.12	Putative cytochrome b5-like heme/steroid binding domain-containing protein OS=Ro...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR018506	Cyt_B5_heme-BS
IPR036400	Cyt_B5-like_heme/steroid_sf
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0010319	stromule
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G019280.1	CsGy6G019280.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber Gy14 genome (v2)
Date Performed: 2018-09-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 43.47 coord: 40..54 score: 51.92 coord: 30..40 score: 55.65 coord: 55..62 score: 44.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 2.4E-31 score: 120.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 e-value: 2.8E-25 score: 88.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 28.714
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	G3DSA:3.10.120.10		coord: 2..87 e-value: 5.2E-34 score: 117.9
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A	coord: 1..131
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..131
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsGy6G019280	Cla022031	Watermelon (97103) v1	cgybwmB427
CsGy6G019280	Csa6G366540	Cucumber (Chinese Long) v2	cgybcuB281
CsGy6G019280	MELO3C011415	Melon (DHL92) v3.5.1	cgybmeB406
CsGy6G019280	ClCG08G010480	Watermelon (Charleston Gray)	cgybwcgB454
CsGy6G019280	CSPI06G19730	Wild cucumber (PI 183967)	cgybcpiB287
CsGy6G019280	CmaCh05G004510	Cucurbita maxima (Rimu)	cgybcmaB844
CsGy6G019280	CmoCh05G004770	Cucurbita moschata (Rifu)	cgybcmoB825
CsGy6G019280	Cp4.1LG11g03860	Cucurbita pepo (Zucchini)	cgybcpeB714
CsGy6G019280	MELO3C011415.2	Melon (DHL92) v3.6.1	cgybmedB405
CsGy6G019280	CsaV3_6G033730	Cucumber (Chinese Long) v3	cgybcucB298
CsGy6G019280	Cla97C08G153330	Watermelon (97103) v2	cgybwmbB463
CsGy6G019280	Bhi04G000922	Wax gourd	cgybwgoB547
CsGy6G019280	Carg09262	Silver-seed gourd	carcgybB0156

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsGy6G019280	Silver-seed gourd	carcgybB0649
CsGy6G019280	Cucumber (Chinese Long) v3	cgybcucB250
CsGy6G019280	Cucumber (Gy14) v2	cgybcgybB037
CsGy6G019280	Cucurbita maxima (Rimu)	cgybcmaB714
CsGy6G019280	Cucurbita moschata (Rifu)	cgybcmoB698
CsGy6G019280	Cucurbita pepo (Zucchini)	cgybcpeB875
CsGy6G019280	Bottle gourd (USVL1VR-Ls)	cgyblsiB437