Cucsa.327920 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.327920
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Cytochrome b5
Location	scaffold03159 : 215362 .. 217903 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCATCGTGATACTCACATTTTTTGTTGGGCTTTTTGTTTCTTTAGTAAATACTCCTATGGCCCAACGAAGGACTGGATGAGGCCCAAAACCCACCAAGAAGTTCGATATTTCGAGGGCCAGTTCTTCAAAAAAGAGAAGAAAAaGAAAAAAaTCCGATCGTGGTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTACGTCCCTGACAATCTTCATAGCCTCCTATAGTTTTATTTTCCTCATCCCTATATGATTCTTATGTAATTTCCTCTCTGGGTTGTTTTCGGTTCATGCTTGTTTTTATTCTACTTGTGATTCTAGAGATGTAAACTGAATTCAATTTTTATTGTTGATGATTGTCTTTTTGGGTTGAAGAGGTGGGGAGTTGAAGCTATAGTTTGACTGGAATTACCTGAATTTGTCAATGTAATGGAGAGAATAGCAGCACACAAGCATATGATGTCCAAGATTCTTTGATTTATGTTGTTGATGGTTTGTAGGTTATCTGGATTTTAGGTAAGTGTTGATTATGATAGTTGTAAATGATAAGGAACATAAGTTAATTCTAAATGAGATCCTATTTAGAATAAAGTAATTATCTGTCAATATAGGTATGAATCATAAACTACTTTAAGAGGTGAAATCTGAGAAAGGCTATTGGCTTGAATTTCTCTTCTCCATCTAAAATATATTCTTCAACACTCTAAGATTCGTGTTGTTCCCACTCTTGGAGGCAGAGTGGAGATAACAGGATTCAGTGTTTTTGTGAATTAGGCCTGGGCTGTTTCATTCTTTTCGAAAAGAATGATTAGGACTTTCCCCTTCTATTAGCTAAGGAAAATAAAATTTACTAAGCCCAAATGTTTTTATTTTCTATAGTCCCACTTCAACTATGCTTTTACATTATTTATGTAGCACAAATTTAAGTTTGCCCCTTAGAAAACCCAAAAAAAaGTTTGCACTTCCTTTTTtCCTCCTTACGCTTGAGCGCTGAAAGACTATTGTTGCACTTTATTAGGCCCCGAGCTTTAAAAAACATTGGCCCAATTAGAGTAGAAGGGAAAAAaGGAAGGATGGATTTGAAAACCCAACCAACAACATGGTTCACATTTGAATCAGTAGATATATTTTAATGCTCAATTCATTGTCATGTCAAACGTTTAGGATTAACAATACTAAAGTAATGGGTTACTGTACTGAATTCAAATAAGACTAAAAGTTTCAATCTCCTCATAGAACTTATGTTAGTGTTTGAATCTGAGAATTTGCTTGAGTGGATATGTCATTTCTTATGATTTGTTGATTTCAAATTCTTGAGAGGTAATTGGCTTTTTCGTGGGGTCTTTGAAACATTGGCCCACCATTTCCAGAATCTTTTTAAAATTTATGTGCTCATTTTCTTTATGGCAGCCTTTCAGTCAAGTCATCTAGTTTGTCAAAAATTGTGAATGTCTTTTCTATGAAAGAATTGTGTTTGAGGATGAGAAGGATGAGTCCTATCACTCATATTTTTCCTAGCTCACCATCTTTGCCACAAAAAACTGATATTTTCATTTTTACTTTATATTTGAGTCCCATGATCAGTTTTCTGTCGACCATTGACACCATTCTACGACACGTCAACCTCTTCTAACCATTTTGTGTGTTTTCTTTTAACGTATCTTCTCCAAGAGGACACACTTTGTCATTGCTTTAAATTGATATATTATCATTGTTAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGTATGATTTTACTTTGTAATGTAAATTTCTCTACCAAATTTGAACAGTTACAAATATAACTTTTTGTCCTATCAAAGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGATTCCTGTGCCAATTAAAGGCTGTTGGCTTGTTGAGTTGAGAGAGTTGGTCTCATTTTTGCTTCTTGAAAAACTTCATATTCGTTTCAATATATTCATACAATGATTGTGGTAGGTTCTTTGTTCCTTAGTAGTACTGTAGAAAGGATTATTTTCTTGTTAATTGGTAGGTTTGTTTATTTGTTTCCATAGTTTCATTAGATGGGAGTTGGGAAAATGGTTTCGGGTTCAATGGAGTTTGATCCAACTCCATTGTCTCTTTTTGACCAAACTATATATACTGTTCTCTACATGCAGCAGTTAATTTTGAGCCTGAAGACATGTGACATCTTA

mRNA sequence

TTCATCGTGATACTCACATTTTTTGTTGGGCTTTTTGTTTCTTTAGTAAATACTCCTATGGCCCAACGAAGGACTGGATGAGGCCCAAAACCCACCAAGAAGTTCGATATTTCGAGGGCCAGTTCTTCAAAAAAGAGAAGAAAAAGAAAAAAATCCGATCGTGGTCGAACCAACCCGCGGCGGCGATTAGTCGACCAATCGCCAAAAGGGATCGATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGATTCCTGTGCCAATTAAAGGCTGTTGGCTTGTTGAGTTGAGAGAGTTGGTCTCATTTTTGCTTCTTGAAAAACTTCATATTCGTTTCAATATATTCATACAATGATTGTGGTAGGTTCTTTGTTCCTTAGTAGTACTGTAGAAAGGATTATTTTCTTGTTAATTGGTAGGTTTGTTTATTTGTTTCCATAGTTTCATTAGATGGGAGTTGGGAAAATGGTTTCGGGTTCAATGGAGTTTGATCCAACTCCATTGTCTCTTTTTGACCAAACTATATATACTGTTCTCTACATGCAGCAGTTAATTTTGAGCCTGAAGACATGTGACATCTTA

Coding sequence (CDS)

ATGCCGAGCATTTCGGCCCTCTATTCCATTCAAGAAGTCTCACAGCACAGTTCCAACGACGACTGTTGGATCATTATCGATGGCAAGGTATACGATTTAACTTCATATTTGGATGAGCATCCTGGTGGAGATGATGTTATTGTGGCTGCAACTGGCAGAGATGCCACGGATGATTTTGAAGATGCTGGTCATAGCAAAGATGCTAGGGAGTTGATGGAGAAGTTCTATATCGGCTTGCTTGATACATCTTCCTTGGATAGCCTAAAACTTGAAACGAATCAAGTAGACGGTTATGCAACTCGGGTTCAGACCTTGACAAAGCAATACTGGAAGGCCCCTGTTGCAGTAATTGGTATTTCTGTGGTACTTGGTTGGGCATACTTGCGCAAAAAGTGA

Protein sequence

MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGISVVLGWAYLRKK*

Homology

[Close]

BLAST of Cucsa.327920 vs. Swiss-Prot
Match: CYB5A_ARATH (Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 8.9e-39
Identity = 74/134 (55.22%), Postives = 100/134 (74.63%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLK----LETNQVDGYATRVQTLTKQYWKAPVAV 120
           DAGHSKDARELMEK++IG LD SSL  +      + +Q      ++  LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 IGISVVLGWAYLRK 131
           I ISV +   + RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of Cucsa.327920 vs. Swiss-Prot
Match: CYB5D_ARATH (Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.5e-25
Identity = 46/79 (58.23%), Postives = 69/79 (87.34%), Query Frame = 1


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMEKFYIGLLDTSSL 86
           A+ +++++Y+G +DT+++
Sbjct: 67 TAKAMLDEYYVGDIDTATV 85

[Close]

BLAST of Cucsa.327920 vs. Swiss-Prot
Match: CYB5_BRAOB (Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.9e-25
Identity = 52/127 (40.94%), Postives = 87/127 (68.50%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           M S   +   +EVSQH+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAV 120
           D GHS  AR++MEK+YIG +D+S++ + +     V     + +T    +    +  P+ +
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYVAPVQPAYNQDKTPEFMIKILQFLVPILI 120

Query: 121 IGISVVL 124
           +G+++V+
Sbjct: 121 LGLALVV 127

[Close]

BLAST of Cucsa.327920 vs. Swiss-Prot
Match: CYB5_ORYSJ (Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 3.3e-25
Identity = 49/121 (40.50%), Postives = 88/121 (72.73%), Query Frame = 1


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           +Y+++EV++H+S DDCW+II GKVY+++ +L++HPGGDDV++++TG+DATDDFED GHS 
Sbjct: 8   VYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHST 67

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAVIGISVV 124
            AR +M+++Y+G +DTS++ +          +  + +T    +    +  P+A++G++V 
Sbjct: 68  TARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVA 127

[Close]

BLAST of Cucsa.327920 vs. Swiss-Prot
Match: CYB5_BOROF (Cytochrome b5 OS=Borago officinalis PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.3e-25
Identity = 44/81 (54.32%), Postives = 72/81 (88.89%), Query Frame = 1


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EV+QH+++ DCW+II+GKVYD+T +L++HPGGDDV+++ATG+DATDDFED GHS 
Sbjct: 4  IFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHSS 63

Query: 67 DARELMEKFYIGLLDTSSLDS 88
           A+ +++++Y+G +D+SS+ S
Sbjct: 64 SAKAMLDEYYVGDIDSSSIPS 84

[Close]

BLAST of Cucsa.327920 vs. TrEMBL
Match: A0A0A0KIR1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G366540 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGWAYLRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of Cucsa.327920 vs. TrEMBL
Match: A0A061E9I3_THECC (Cytochrome B5, n6,ATCB5-A,CB5-A OS=Theobroma cacao GN=TCM_011490 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 9.3e-43
Identity = 84/134 (62.69%), Postives = 110/134 (82.09%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY++QE SQH+S +DCW++IDGKVYD+TSYLDEHPGGDDV++A+TG+DATDDFE
Sbjct: 1   MPTLTKLYTMQEASQHNSKEDCWVVIDGKVYDVTSYLDEHPGGDDVVLASTGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETN---QVDGYATRVQTLTKQYWKAPVAVI 120
           DAGHSK A+ELM+ F IG LDTSS    +LE +   +   Y+ ++  LTKQYW  PVAV+
Sbjct: 61  DAGHSKSAKELMQTFCIGELDTSSPIIPELEISSKKETTDYSQKLMGLTKQYWAVPVAVV 120

Query: 121 GISVVLGWAYLRKK 132
           GISVV+G+ YLR+K
Sbjct: 121 GISVVVGFLYLRRK 134

[Close]

BLAST of Cucsa.327920 vs. TrEMBL
Match: A0A0B0MRW9_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_27157 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.7e-41
Identity = 82/134 (61.19%), Postives = 108/134 (80.60%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY++QE SQH++ DDCWI+IDGKVYD+TSYLDEHPGGDDV++ +TG+DATDDFE
Sbjct: 1   MPTLTKLYTMQEASQHNTKDDCWIVIDGKVYDVTSYLDEHPGGDDVVLESTGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETN---QVDGYATRVQTLTKQYWKAPVAVI 120
           DAGHSK A+EL++ F IG LDTSS    +LE +   +   Y+ ++  LTKQYW  PVA++
Sbjct: 61  DAGHSKSAKELLQSFCIGELDTSSPIIPELEISSKKEAVKYSEKLMDLTKQYWTIPVAIV 120

Query: 121 GISVVLGWAYLRKK 132
           GISVV+G+ YL KK
Sbjct: 121 GISVVVGFLYLHKK 134

[Close]

BLAST of Cucsa.327920 vs. TrEMBL
Match: W9QWA3_9ROSA (Putative cytochrome b5 isoform 2 OS=Morus notabilis GN=L484_026450 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 2.3e-41
Identity = 80/132 (60.61%), Postives = 105/132 (79.55%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP ++ LY++ E S H++ DDCW++IDGKVYD++SYLD+HPGGDDV++AATGRDAT+DFE
Sbjct: 1   MPQLTKLYTMAEASDHNTKDDCWVVIDGKVYDVSSYLDDHPGGDDVVLAATGRDATEDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLK-LETNQVDGYATRVQTLTKQYWKAPVAVIGI 120
           DAGHSK A+ELME F IG LDTS++  L+ +   Q   YA ++  LTKQYW  P  V+G+
Sbjct: 61  DAGHSKSAKELMETFCIGELDTSAIPELEIISKKQRRDYAQKLVELTKQYWVVPTTVVGV 120

Query: 121 SVVLGWAYLRKK 132
           SVV+G+ YLRKK
Sbjct: 121 SVVVGFLYLRKK 132

[Close]

BLAST of Cucsa.327920 vs. TrEMBL
Match: A0A0D2VBK0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G095300 PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 3.9e-41
Identity = 81/134 (60.45%), Postives = 108/134 (80.60%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY++QE SQH++ DDCWI+IDGKVYD+TSYLDEHPGGDDV++ +TG+DATDDFE
Sbjct: 1   MPTLTKLYTMQEASQHNTKDDCWIVIDGKVYDVTSYLDEHPGGDDVVLESTGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETN---QVDGYATRVQTLTKQYWKAPVAVI 120
           DAGHSK A+EL++ F IG LDTSS    +LE +   +   Y+ ++  LTKQYW  PVA++
Sbjct: 61  DAGHSKSAKELLQSFCIGELDTSSPIIPELEISSKREAVKYSEKLMDLTKQYWTIPVAIV 120

Query: 121 GISVVLGWAYLRKK 132
           GISV++G+ YL KK
Sbjct: 121 GISVLVGFLYLHKK 134

[Close]

BLAST of Cucsa.327920 vs. TAIR10
Match: AT1G26340.1 (AT1G26340.1 cytochrome B5 isoform A)

HSP 1 Score: 161.0 bits (406), Expect = 5.0e-40
Identity = 74/134 (55.22%), Postives = 100/134 (74.63%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LYS++E + H+  DDCW++IDGKVYD++SY+DEHPGGDDV++A  G+DATDDFE
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLK----LETNQVDGYATRVQTLTKQYWKAPVAV 120
           DAGHSKDARELMEK++IG LD SSL  +      + +Q      ++  LTKQYW  PV++
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

Query: 121 IGISVVLGWAYLRK 131
           I ISV +   + RK
Sbjct: 121 ITISVAVSVLFSRK 134

[Close]

BLAST of Cucsa.327920 vs. TAIR10
Match: AT5G48810.1 (AT5G48810.1 cytochrome B5 isoform D)

HSP 1 Score: 117.1 bits (292), Expect = 8.3e-27
Identity = 46/79 (58.23%), Postives = 69/79 (87.34%), Query Frame = 1


Query: 7  LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
          ++++ EVSQHSS  DCWI+IDGKVYD+T +LD+HPGGD+VI+ +TG+DATDDFED GHS 
Sbjct: 7  VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 67 DARELMEKFYIGLLDTSSL 86
           A+ +++++Y+G +DT+++
Sbjct: 67 TAKAMLDEYYVGDIDTATV 85

[Close]

BLAST of Cucsa.327920 vs. TAIR10
Match: AT2G32720.1 (AT2G32720.1 cytochrome B5 isoform B)

HSP 1 Score: 115.5 bits (288), Expect = 2.4e-26
Identity = 49/119 (41.18%), Postives = 86/119 (72.27%), Query Frame = 1


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           ++++ EVS+H+   DCWI+I+GKVY++T +L++HPGGDDV++++TG+DATDDFED GHS+
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQT----LTKQYWKAPVAVIGISV 122
            ARE+ME++Y+G +D +++      T     +  + +T    +    +  P+A++G++V
Sbjct: 67  SAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAV 125

[Close]

BLAST of Cucsa.327920 vs. TAIR10
Match: AT5G53560.1 (AT5G53560.1 cytochrome B5 isoform E)

HSP 1 Score: 113.2 bits (282), Expect = 1.2e-25
Identity = 46/89 (51.69%), Postives = 72/89 (80.90%), Query Frame = 1


Query: 1  MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
          M S   + S +EVS+H+   DCW+II GKVYD+T ++D+HPGGD+V++++TG+DAT+DFE
Sbjct: 1  MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61 DAGHSKDARELMEKFYIGLLDTSSLDSLK 90
          D GHS  AR++M+K++IG +D+SS+ + +
Sbjct: 61 DVGHSDTARDMMDKYFIGEIDSSSVPATR 89

[Close]

BLAST of Cucsa.327920 vs. TAIR10
Match: AT2G46650.1 (AT2G46650.1 cytochrome B5 isoform C)

HSP 1 Score: 107.8 bits (268), Expect = 5.0e-24
Identity = 50/123 (40.65%), Postives = 82/123 (66.67%), Query Frame = 1


Query: 7   LYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFEDAGHSK 66
           L S  +V++H   +DCWI+I GKVYD+++++DEHPGGD+V++A TG+DA+ DFED  HSK
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  DARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWK------APVAVIGIS 124
           DA+ELM+K+ IG +D S++   +      +  +T  +T  ++  K       P+ ++G++
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

[Close]

BLAST of Cucsa.327920 vs. NCBI nr
Match: gi|449452839|ref|XP_004144166.1| (PREDICTED: cytochrome b5 isoform A [Cucumis sativus])

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-68
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           VVLGWAYLRKK
Sbjct: 121 VVLGWAYLRKK 131

[Close]

BLAST of Cucsa.327920 vs. NCBI nr
Match: gi|659089317|ref|XP_008445442.1| (PREDICTED: cytochrome B5 isoform D [Cucumis melo])

HSP 1 Score: 255.8 bits (652), Expect = 4.2e-65
Identity = 124/131 (94.66%), Postives = 127/131 (96.95%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MPSISALYSIQEVSQHSS+DDCWIIIDGKVYDLTSYLDEHPGGDD+IV ATGRDATDDFE
Sbjct: 1   MPSISALYSIQEVSQHSSSDDCWIIIDGKVYDLTSYLDEHPGGDDIIVTATGRDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSKDARELMEKFYIGLLDTSS DSLKLETNQVD YATRVQTLTKQYWKAPVAVIGIS
Sbjct: 61  DAGHSKDARELMEKFYIGLLDTSSSDSLKLETNQVDSYATRVQTLTKQYWKAPVAVIGIS 120

Query: 121 VVLGWAYLRKK 132
           +VLGW YLRKK
Sbjct: 121 MVLGWVYLRKK 131

[Close]

BLAST of Cucsa.327920 vs. NCBI nr
Match: gi|1009154429|ref|XP_015895166.1| (PREDICTED: cytochrome b5 isoform A [Ziziphus jujuba])

HSP 1 Score: 182.6 bits (462), Expect = 4.6e-43
Identity = 83/131 (63.36%), Postives = 108/131 (82.44%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY+IQE SQH+S DDCW++IDGKVYD++SYLDEHPGGDDV     G+DA ++FE
Sbjct: 1   MPTLTKLYTIQEASQHNSKDDCWVVIDGKVYDVSSYLDEHPGGDDVFFDVRGKDAIEEFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIGIS 120
           DAGHSK ARELMEKF IG LDT+ +  L++ T++ +GY+ +V  LTKQYW  PVA++GIS
Sbjct: 61  DAGHSKSARELMEKFCIGELDTTPIPELEI-TSKKEGYSKKVADLTKQYWAVPVAIVGIS 120

Query: 121 VVLGWAYLRKK 132
           VV+G+ YLRKK
Sbjct: 121 VVVGFLYLRKK 130

[Close]

BLAST of Cucsa.327920 vs. NCBI nr
Match: gi|590698925|ref|XP_007045813.1| (Cytochrome B5, n6,ATCB5-A,CB5-A [Theobroma cacao])

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-42
Identity = 84/134 (62.69%), Postives = 110/134 (82.09%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ LY++QE SQH+S +DCW++IDGKVYD+TSYLDEHPGGDDV++A+TG+DATDDFE
Sbjct: 1   MPTLTKLYTMQEASQHNSKEDCWVVIDGKVYDVTSYLDEHPGGDDVVLASTGKDATDDFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSSLDSLKLETN---QVDGYATRVQTLTKQYWKAPVAVI 120
           DAGHSK A+ELM+ F IG LDTSS    +LE +   +   Y+ ++  LTKQYW  PVAV+
Sbjct: 61  DAGHSKSAKELMQTFCIGELDTSSPIIPELEISSKKETTDYSQKLMGLTKQYWAVPVAVV 120

Query: 121 GISVVLGWAYLRKK 132
           GISVV+G+ YLR+K
Sbjct: 121 GISVVVGFLYLRRK 134

[Close]

BLAST of Cucsa.327920 vs. NCBI nr
Match: gi|698478085|ref|XP_009786222.1| (PREDICTED: cytochrome b5 isoform B [Nicotiana sylvestris])

HSP 1 Score: 180.3 bits (456), Expect = 2.3e-42
Identity = 81/133 (60.90%), Postives = 110/133 (82.71%), Query Frame = 1


Query: 1   MPSISALYSIQEVSQHSSNDDCWIIIDGKVYDLTSYLDEHPGGDDVIVAATGRDATDDFE 60
           MP+++ L++++E SQH++ DDCW++IDGKVYD++SYLDEHPGGDDV++AATG+DATD+FE
Sbjct: 1   MPTLTKLFTMEEASQHNTKDDCWVVIDGKVYDVSSYLDEHPGGDDVVLAATGKDATDEFE 60

Query: 61  DAGHSKDARELMEKFYIGLLDTSS--LDSLKLETNQVDGYATRVQTLTKQYWKAPVAVIG 120
           DAGHSKDARELMEKF+IG LD++S  +  L++          +V+ +TKQYW  PVAV+G
Sbjct: 61  DAGHSKDARELMEKFFIGELDSTSPPIPELEIVKKAAKNIPQKVKEITKQYWFIPVAVVG 120

Query: 121 ISVVLGWAYLRKK 132
           ISVV+G+ Y RKK
Sbjct: 121 ISVVVGFLYTRKK 133

The following BLAST results are available for this feature:

BLAST of Cucsa.327920 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CYB5A_ARATH	8.9e-39	55.22	Cytochrome b5 isoform A OS=Arabidopsis thaliana GN=CB5-A PE=1 SV=1	[more]
CYB5D_ARATH	1.5e-25	58.23	Cytochrome B5 isoform D OS=Arabidopsis thaliana GN=CYTB5-D PE=1 SV=1	[more]
CYB5_BRAOB	1.9e-25	40.94	Cytochrome b5 OS=Brassica oleracea var. botrytis GN=CYB5 PE=1 SV=1	[more]
CYB5_ORYSJ	3.3e-25	40.50	Cytochrome b5 OS=Oryza sativa subsp. japonica GN=Os05g0108800 PE=2 SV=2	[more]
CYB5_BOROF	4.3e-25	54.32	Cytochrome b5 OS=Borago officinalis PE=2 SV=1	[more]

BLAST of Cucsa.327920 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIR1_CUCSA	1.3e-68	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G366540 PE=3 SV=1	[more]
A0A061E9I3_THECC	9.3e-43	62.69	Cytochrome B5, n6,ATCB5-A,CB5-A OS=Theobroma cacao GN=TCM_011490 PE=3 SV=1	[more]
A0A0B0MRW9_GOSAR	1.7e-41	61.19	Uncharacterized protein OS=Gossypium arboreum GN=F383_27157 PE=3 SV=1	[more]
W9QWA3_9ROSA	2.3e-41	60.61	Putative cytochrome b5 isoform 2 OS=Morus notabilis GN=L484_026450 PE=3 SV=1	[more]
A0A0D2VBK0_GOSRA	3.9e-41	60.45	Uncharacterized protein OS=Gossypium raimondii GN=B456_013G095300 PE=3 SV=1	[more]

BLAST of Cucsa.327920 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G26340.1	5.0e-40	55.22	cytochrome B5 isoform A	[more]
AT5G48810.1	8.3e-27	58.23	cytochrome B5 isoform D	[more]
AT2G32720.1	2.4e-26	41.18	cytochrome B5 isoform B	[more]
AT5G53560.1	1.2e-25	51.69	cytochrome B5 isoform E	[more]
AT2G46650.1	5.0e-24	40.65	cytochrome B5 isoform C	[more]

BLAST of Cucsa.327920 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449452839\|ref\|XP_004144166.1\|	1.8e-68	100.00	PREDICTED: cytochrome b5 isoform A [Cucumis sativus]	[more]
gi\|659089317\|ref\|XP_008445442.1\|	4.2e-65	94.66	PREDICTED: cytochrome B5 isoform D [Cucumis melo]	[more]
gi\|1009154429\|ref\|XP_015895166.1\|	4.6e-43	63.36	PREDICTED: cytochrome b5 isoform A [Ziziphus jujuba]	[more]
gi\|590698925\|ref\|XP_007045813.1\|	1.3e-42	62.69	Cytochrome B5, n6,ATCB5-A,CB5-A [Theobroma cacao]	[more]
gi\|698478085\|ref\|XP_009786222.1\|	2.3e-42	60.90	PREDICTED: cytochrome b5 isoform B [Nicotiana sylvestris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd
IPR018506	Cyt_B5_heme-BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0020037	heme binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0009526	plastid envelope
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.327920.1	Cucsa.327920.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 9.8E-14coord: 40..54 score: 9.8E-14coord: 30..40 score: 9.8E-14coord: 55..62 score: 9.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 7..86 score: 1.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 11..80 score: 2.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 score: 2.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROFILE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 28
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..85 score: 1.31
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43 scor
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 7..131 score: 1.5
None	No IPR available	PANTHER	PTHR19359:SF25	CYTOCHROME B5 ISOFORM A-RELATED	coord: 7..131 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.327920	Cla022031	Watermelon (97103) v1	cgywmB593
Cucsa.327920	Csa6G366540	Cucumber (Chinese Long) v2	cgycuB466
Cucsa.327920	MELO3C011415	Melon (DHL92) v3.5.1	cgymeB539
Cucsa.327920	ClCG08G010480	Watermelon (Charleston Gray)	cgywcgB565
Cucsa.327920	CSPI06G19730	Wild cucumber (PI 183967)	cgycpiB492
Cucsa.327920	CmaCh05G004510	Cucurbita maxima (Rimu)	cgycmaB0909
Cucsa.327920	CmoCh05G004770	Cucurbita moschata (Rifu)	cgycmoB0907
Cucsa.327920	Cp4.1LG11g03860	Cucurbita pepo (Zucchini)	cgycpeB0863
Cucsa.327920	MELO3C011415.2	Melon (DHL92) v3.6.1	cgymedB539
Cucsa.327920	CsaV3_6G033730	Cucumber (Chinese Long) v3	cgycucB508
Cucsa.327920	Cla97C08G153330	Watermelon (97103) v2	cgywmbB569
Cucsa.327920	Bhi04G000922	Wax gourd	cgywgoB662
Cucsa.327920	Carg09262	Silver-seed gourd	carcgyB0175

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.327920	Cucumber (Chinese Long) v3	cgycucB507
Cucsa.327920	Cucumber (Gy14) v1	cgycgyB066
Cucsa.327920	Cucurbita maxima (Rimu)	cgycmaB0906
Cucsa.327920	Cucurbita moschata (Rifu)	cgycmoB0904
Cucsa.327920	Melon (DHL92) v3.5.1	cgymeB538
Cucsa.327920	Watermelon (Charleston Gray)	cgywcgB564
Cucsa.327920	Watermelon (97103) v1	cgywmB595
Cucsa.327920	Cucurbita pepo (Zucchini)	cgycpeB0866
Cucsa.327920	Bottle gourd (USVL1VR-Ls)	cgylsiB535
Cucsa.327920	Silver-seed gourd	carcgyB0729