Carg08355 (gene) Silver-seed gourd

BLAST of Carg08355 vs. NCBI nr
Match: XP_022929776.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 983.0 bits (2540), Expect = 4.7e-283
Identity = 519/607 (85.50%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355 vs. NCBI nr
Match: XP_022997404.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 979.5 bits (2531), Expect = 5.2e-282
Identity = 516/607 (85.01%), Postives = 519/607 (85.50%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LA+RRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LASRRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFA+ENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFAMENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMS+LLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSTLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVRSKGSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRSKGSKISEDDIKKYISDQ------------------------------------ 531

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 596
                                                   VIYYKKIRKVFFVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVFFVDSIPVAP 531

BLAST of Carg08355 vs. NCBI nr
Match: XP_023546819.1 (4-coumarate--CoA ligase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 976.9 bits (2524), Expect = 3.4e-281
Identity = 518/617 (83.95%), Postives = 523/617 (84.76%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCF+HLSQFHHRPCLINAATGQIHTYAAVD
Sbjct: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFQHLSQFHHRPCLINAATGQIHTYAAVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ
Sbjct: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           AATARPKVIITQAEFVEKVREFA+ENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN
Sbjct: 121 AATARPKVIITQAEFVEKVREFALENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY
Sbjct: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL
Sbjct: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA
Sbjct: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKIIHPETGVSLPRNHP            GYLNNKEATEKTIDKNGWLHTG
Sbjct: 361 CGTVVRNAEMKIIHPETGVSLPRNHPGEICIRGSQIMKGYLNNKEATEKTIDKNGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP
Sbjct: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
           AAFVVR++GSKISEDDIKKYISDQ                                    
Sbjct: 481 AAFVVRTEGSKISEDDIKKYISDQ------------------------------------ 540

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 600
                                                   VIYYKKIRKV FVDSIPVAP
Sbjct: 541 ----------------------------------------VIYYKKIRKVLFVDSIPVAP 540

Query: 601 SGKILRKDRHQIQLPSG 606
           SGKILRK   + QL SG
Sbjct: 601 SGKILRKVL-KAQLESG 540

BLAST of Carg08355 vs. NCBI nr
Match: XP_008452293.1 (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 880.2 bits (2273), Expect = 4.3e-252
Identity = 460/611 (75.29%), Postives = 489/611 (80.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGW 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHP            GYLNN+EATEKTIDK+GW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPI 540
           GEIP AFVVRSKGSKISE+DIK YISDQ                                
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQ-------------------------------- 535

Query: 541 HPFSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSI 596
                                                       VIYYK+IRKVFFVDSI
Sbjct: 541 --------------------------------------------VIYYKRIRKVFFVDSI 535

BLAST of Carg08355 vs. NCBI nr
Match: XP_004149075.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis sativus] >KGN61091.1 hypothetical protein Csa_2G048330 [Cucumis sativus])

HSP 1 Score: 868.2 bits (2242), Expect = 1.7e-248
Identity = 455/605 (75.21%), Postives = 487/605 (80.50%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N +DFIF+SKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG+IHTYA V+LAARR 
Sbjct: 8   NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRF 67

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQA  A+ 
Sbjct: 68  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKV 127

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPAVKINSN 186
           KVIITQ EFVEKV EFAVE+GVKI+CTD    S   GCL+FSE++EADENEIPAVKINS+
Sbjct: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSS 187

Query: 187 DVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSL 246
           DVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++N  DV+IC+LPLFHIYSL
Sbjct: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247

Query: 247 NSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 306
           NSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS
Sbjct: 248 NSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 307

Query: 307 IRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACG 366
           IRIVMSGAAPMGK LEDTVKA+LPNA LGQGYGMTEAGPVLSMCLGFAKE F+ KSGACG
Sbjct: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367

Query: 367 TVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDL 426
           TVVRNAEMKIIHP+T +SLPRN+P            GYLNNKEATEKTIDK+GWLHTGDL
Sbjct: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDL 427

Query: 427 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAA 486
           G+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP A
Sbjct: 428 GYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVA 487

Query: 487 FVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGS 546
           FVVRSKGSKISE+DIK YISDQ                                      
Sbjct: 488 FVVRSKGSKISENDIKNYISDQ-------------------------------------- 536

Query: 547 SILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSG 596
                                                 VIYYKKIRKVFFVDSIP+APSG
Sbjct: 548 --------------------------------------VIYYKKIRKVFFVDSIPMAPSG 536

BLAST of Carg08355 vs. TAIR10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1)

HSP 1 Score: 641.3 bits (1653), Expect = 6.1e-184
Identity = 333/610 (54.59%), Postives = 420/610 (68.85%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N  D IFRSKLPDIYIPNHL LH Y F+++S+F  +PCLIN  TG ++TY+ V + +R++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A    K+GV Q DV+MLLL N PEFV +FL AS+ GA +T ANP + PAEI KQA  +  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTDSP-----PVGCLRFSEILEAD---ENEIPAVK 186
           K+IIT+A +V+K++    ++GV I+C D       P GCLRF+E+ ++       I +V+
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 198

Query: 187 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 246
           I+ +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  +S+DV++C+LP+FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 247 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 306
           IY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK+ +  +Y
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 318

Query: 307 DLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKS 366
           DLSSIR+V SGAAP+GK LED V A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KS
Sbjct: 319 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 378

Query: 367 GACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLH 426
           GACGTVVRNAEMKI+ P+TG SL RN P            GYLNN  AT +TIDK+GWLH
Sbjct: 379 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 438

Query: 427 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGE 486
           TGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+++  AVV M++EAAGE
Sbjct: 439 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 498

Query: 487 IPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHP 546
           +P AFVV+SK S++SEDD+K+++S Q                                  
Sbjct: 499 VPVAFVVKSKDSELSEDDVKQFVSKQ---------------------------------- 552

Query: 547 FSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPV 597
                                                     V++YK+I KVFF +SIP 
Sbjct: 559 ------------------------------------------VVFYKRINKVFFTESIPK 552

BLAST of Carg08355 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 637.9 bits (1644), Expect = 6.7e-183
Identity = 333/605 (55.04%), Postives = 418/605 (69.09%), Query Frame = 0

Query: 8   AQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVA 67
           + D IFRS+LPDIYIPNHLPLH Y FE++S+F  +PCLIN  TG+++TYA V + +R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  TGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPK 127
            GL  +GV Q DV+M+LL NSPE V  FL AS+ GAI+T+ANP + PAEI KQA  +  K
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 VIITQAEFVEKVREFAVENGVKIICTDSP--PVGCLRFSEILEADENEIPAV--KINSND 187
           +I+TQ+ +V+K++     +GV I+ TDS   P  CLRFSE+ +++E  + ++  KI+  D
Sbjct: 138 LIVTQSRYVDKIKNLQ-NDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPED 197

Query: 188 VVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLN 247
           VVALPFSSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  N +DV++C+LP+FHIY+LN
Sbjct: 198 VVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALN 257

Query: 248 SVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSI 307
           S++LC LRVGA ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+  +YDLSS+
Sbjct: 258 SIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSV 317

Query: 308 RIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGT 367
           R+V SGAAP+GK LED + A+ PNA LGQGYGMTEAGPVL+M LGFAKEPF  KSGACGT
Sbjct: 318 RMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGT 377

Query: 368 VVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDLG 427
           VVRNAEMKI+ P+TG SLPRN P            GYLN+  AT  TIDK+GWLHTGD+G
Sbjct: 378 VVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVG 437

Query: 428 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAF 487
           FIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P ++  AVV M++E AGE+P AF
Sbjct: 438 FIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAF 497

Query: 488 VVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSS 547
           VVRSK S ISED+IK+++S Q                                       
Sbjct: 498 VVRSKDSNISEDEIKQFVSKQ--------------------------------------- 545

Query: 548 ILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGK 597
                                                V++YK+I KVFF DSIP APSGK
Sbjct: 558 -------------------------------------VVFYKRINKVFFTDSIPKAPSGK 545

BLAST of Carg08355 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 602.4 bits (1552), Expect = 3.1e-172
Identity = 316/600 (52.67%), Postives = 404/600 (67.33%), Query Frame = 0

Query: 12  IFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVATGLS 71
           IFRSKLPDI IPNHLPLHTYCFE LS    +PCLI  +TG+ +TY    L  RRVA+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKVIIT 131
           K+G+ +GDVIM+LLQNS EFVF+F+GAS  GA+STTANP Y   E+ KQ  ++  K+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QAEFVEKVREFAVENGVKIICTDSP-PVGCLRFSEILEADENE--IPAVKINSNDVVALP 191
            +++V+K++       + +I TD P P  CL FS ++  DE       V I  +D  ALP
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALP 213

Query: 192 FSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSVLLC 251
           FSSGTTG+PKGV+LTHKSL+TS+AQQVDG+NPN+ L SNDV++C+LPLFHIYSLNSVLL 
Sbjct: 214 FSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLN 273

Query: 252 GLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMS 311
            LR GA +L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP ++ YDLSS+R V+S
Sbjct: 274 SLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLS 333

Query: 312 GAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVVRNA 371
           GAAP+GK L+D+++ RLP A LGQGYGMTEAGPVLSM LGFAKEP   KSG+CGTVVRNA
Sbjct: 334 GAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNA 393

Query: 372 EMKIIHPETGVSLPRNHPG------------YLNNKEATEKTIDKNGWLHTGDLGFIDDD 431
           E+K++H ET +SL  N PG            YLN+ EAT  TID+ GWLHTGD+G++D+D
Sbjct: 394 ELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDED 453

Query: 432 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSK 491
           DEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +++ AAVVP  DE AGE+P AFVVRS 
Sbjct: 454 DEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSN 513

Query: 492 GSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGT 551
           G+ I+E+D+K+Y++ Q                                            
Sbjct: 514 GNDITEEDVKEYVAKQ-------------------------------------------- 555

Query: 552 PLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKD 597
                                           V++YK++ KVFFV SIP +PSGKILRKD
Sbjct: 574 --------------------------------VVFYKRLHKVFFVASIPKSPSGKILRKD 555

BLAST of Carg08355 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 573.9 bits (1478), Expect = 1.2e-163
Identity = 303/620 (48.87%), Postives = 400/620 (64.52%), Query Frame = 0

Query: 2   DSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLS----QFHHRPCLINAATGQIHTYA 61
           D +   + DFIFRSKLPDI+IPNHLPL  Y F+  S          C+I+ ATG+I TYA
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 62  AVDLAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEI 121
            V    RR+A G+ ++G+  GDV+MLLL NSPEF  +FL  +Y GA+STTANP Y   EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 122 KKQAATARPKVIITQAEFVEKVREFAVENGVKIICTD---------SPPVGCLRFSEILE 181
            KQA  +  K+IIT+   V+K+      +GV I+C D         S   GC+ F+E+ +
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLK-NDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQ 197

Query: 182 ADENEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSND 241
           ADE E+   KI+  D VA+P+SSGTTG+PKGVM+THK LVTSIAQ+VDGENPN++  +ND
Sbjct: 198 ADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAND 257

Query: 242 VLICLLPLFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLD 301
           V++C LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K T+ P  PP+VL 
Sbjct: 258 VILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLA 317

Query: 302 FAKNPDIHRYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLG 361
           F K+P+  RYDLSS+RI++SGAA + K LED V+ + PNA  GQGYGMTE+G V +  L 
Sbjct: 318 FIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLA 377

Query: 362 FAKEPFEFKSGACGTVVRNAEMKIIHPETGVSLPRNH------------PGYLNNKEATE 421
           FAK PF+ KSGACGTV+RNAEMK++  ETG+SLPRN              GYLN+ EAT 
Sbjct: 378 FAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATA 437

Query: 422 KTIDKNGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAV 481
           +TIDK+GWLHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P++  AAV
Sbjct: 438 RTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAV 497

Query: 482 VPMEDEAAGEIPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLL 541
           V M+DE A E+P AFV RS+GS+++EDD+K Y++ Q                        
Sbjct: 498 VAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQ------------------------ 557

Query: 542 QSVWDPPIHPFSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIR 597
                                                               V++YK+I+
Sbjct: 558 ----------------------------------------------------VVHYKRIK 559

BLAST of Carg08355 vs. TAIR10
Match: AT1G62940.1 (acyl-CoA synthetase 5)

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-91
Identity = 189/506 (37.35%), Postives = 291/506 (57.51%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           M+SQ     ++IFRS  P + IP+ L L  +  + + ++      + A TG+  TY  V 
Sbjct: 1   MESQKQEDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVV 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
              +R+A  L+ +G+ +G V++++L N  E+    LG   AG + + ANP    +EIKKQ
Sbjct: 61  RDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADE--NEIPAVK 180
              +  + IIT A   EKV+   +     I+  +    G + + ++LEA +   +    +
Sbjct: 121 VEASGARGIITDATNYEKVKSLGLP---VIVLGEEKIEGAVNWKDLLEAGDKCGDTDNEE 180

Query: 181 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFH 240
           I   D+ ALPFSSGTTG+ KGVMLTH++L+ ++   + G     ++    V + L+P FH
Sbjct: 181 ILQTDLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRS--EMIGQIVTLGLIPFFH 240

Query: 241 IYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 300
           IY +  +    ++    ++ + +YD+   L  +  H+ + AP VPPI+L+  KNP +  +
Sbjct: 241 IYGIVGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEF 300

Query: 301 DLSSIRI--VMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
           DLS +++  VM+ AAP+   L    +A+ PN  + + YG+TE   +        K     
Sbjct: 301 DLSKLKLQSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIA 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRN------------HPGYLNNKEATEKTIDKNGW 420
           K  + G ++ N E+K I P+TG SLP+N              GY  NKE T+KTID+ GW
Sbjct: 361 KRNSVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAA 480
           LHTGD+G+IDDD +IFIVDR+KELIKYKG+QVAPAELEA+L ++P+V   AVVP+ DE A
Sbjct: 421 LHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEA 480

Query: 481 GEIPAAFVVRSKGSKISEDDIKKYIS 491
           GEIPAA VV +  +   E+DI  +++
Sbjct: 481 GEIPAACVVINPKATEKEEDILNFVA 501

BLAST of Carg08355 vs. Swiss-Prot
Match: sp|P31685|4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.0e-205
Identity = 363/608 (59.70%), Postives = 444/608 (73.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +   + D IFRSKLPDIYIP HLPLH+YCFE+LS+F+ RPCLI+ A  +I+TYA V+
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           L +R+VA GL+K+G+ Q D IM+LL N PEFVFAF+GASY GAIST ANPL+ PAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           A  +  K++ITQA F  KV+++A+EN +K+IC DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S+DVL+C+LPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +H YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SS+R VMSGAAP+GK LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKI+ P+TG SLPRN P            GYLN+ EAT +TI+K GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P++S AAVVPM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRS GS I+ED++K +IS Q                                    
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQ------------------------------------ 534

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 597
                                                   VI+YK+I++VFFV+++P +P
Sbjct: 543 ----------------------------------------VIFYKRIKRVFFVETVPKSP 534

BLAST of Carg08355 vs. Swiss-Prot
Match: sp|P31684|4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.3e-204
Identity = 362/608 (59.54%), Postives = 444/608 (73.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD++   + D IFRSKLPDIYIP HLPLH+YCFE+LS+F+ RPCLI+ A  +I+TYA V+
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           L +R+VA GL+K+G+ Q D IM+LL N PEFVFAF+GASY GAIST ANPL+ PAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
           A  +  K++ITQA F  KV+++A+EN +K+IC DS P GC+ FSE++++DE+EIP VKI 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
            +DVVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S+DVL+C+LPLFHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +  YDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SS+R VMSGAAP+GK LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKI+ P+TG SLPRN P            GYLN+ EAT +TI+K GWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           D+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P++S AAVVPM DE AGE+P
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRS GS I+ED++K +IS Q                                    
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQ------------------------------------ 534

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 597
                                                   VI+YK+I++VFFV+++P +P
Sbjct: 543 ----------------------------------------VIFYKRIKRVFFVETVPKSP 534

BLAST of Carg08355 vs. Swiss-Prot
Match: sp|O24145|4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.3e-204
Identity = 362/599 (60.43%), Postives = 438/599 (73.12%), Query Frame = 0

Query: 10  DFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVATG 69
           D IFRSKLPDIYIP HLPLH+YCFE++S+F  RPCLIN A  QI+TYA V+L  R+VA G
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73

Query: 70  LSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKVI 129
           L+K+G+ Q D IM+LL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA  +  K+I
Sbjct: 74  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133

Query: 130 ITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKINSNDVVALPF 189
           ITQ+ FV KV+++A EN VK+IC DS P GCL FSE+ ++DE+EIP VKI  +DVVALP+
Sbjct: 134 ITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDDVVALPY 193

Query: 190 SSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSVLLCG 249
           SSGTTG+PKGVMLTHK LVTS+AQQVDGEN N+ ++S DVL+C+LPLFHIYSLNS+LLCG
Sbjct: 194 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSILLCG 253

Query: 250 LRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMSG 309
           LRVGAAILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P +  YDLSS+R VMSG
Sbjct: 254 LRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSG 313

Query: 310 AAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVVRNAE 369
           AAP+GK LED V+ + PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGACGTVVRNAE
Sbjct: 314 AAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAE 373

Query: 370 MKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDLGFIDDDD 429
           MKI+ P+TG SLPRN P            GYLN+ EAT +TIDK GWLHTGD+GFID+DD
Sbjct: 374 MKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDD 433

Query: 430 EIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSKG 489
           E+FIVDRLKELIKYKG+QVAPAE+EALL ++PN+S AAVVPM+DE AGE+P AFVVRS G
Sbjct: 434 ELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 493

Query: 490 SKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGTP 549
           S I+ED++K +IS Q                                             
Sbjct: 494 SAITEDEVKDFISKQ--------------------------------------------- 536

Query: 550 LRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKD 597
                                          VI+YK++++VFFV+++P +PSGKILRKD
Sbjct: 554 -------------------------------VIFYKRVKRVFFVETVPKSPSGKILRKD 536

BLAST of Carg08355 vs. Swiss-Prot
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 1.5e-203
Identity = 368/603 (61.03%), Postives = 438/603 (72.64%), Query Frame = 0

Query: 6   TNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARR 65
           T   D IFRSKLPDIYIPNHLPLH+YCFE++S+F  RPCLIN A  QI+TYA V+L +R+
Sbjct: 5   TKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRK 64

Query: 66  VATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATAR 125
           VA GL K G+   D IM+LL NSPEFVFAF+GASY GAIST ANPL+ PAE+ KQA  + 
Sbjct: 65  VAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASS 124

Query: 126 PKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKINSNDVV 185
            K+I+TQA  V KV+++A EN VKIIC DS P GCL FS + +A+E++IP V+I  +DVV
Sbjct: 125 AKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVV 184

Query: 186 ALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSV 245
           ALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+ ++S DV++C+LPLFHIYSLNSV
Sbjct: 185 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSV 244

Query: 246 LLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 305
           LLCGLRVGAAILI+QK+D+ S L LIQ +K TI PFVPPIVL  AK+P +  YDLSS+R 
Sbjct: 245 LLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRT 304

Query: 306 VMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVV 365
           VMSGAAP+GK LEDTV+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPFE KSGACGTVV
Sbjct: 305 VMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVV 364

Query: 366 RNAEMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLHTGDLGFI 425
           RNAEMKI+ P+TG SLPRN              GYLN+ EAT +TIDK GWL+TGD+G+I
Sbjct: 365 RNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYI 424

Query: 426 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVV 485
           DDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++PN+S AAVVPM+DE AGE+P AFVV
Sbjct: 425 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVV 484

Query: 486 RSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSIL 545
           RS GS I+ED++K +IS Q                                         
Sbjct: 485 RSNGSTITEDEVKDFISKQ----------------------------------------- 531

Query: 546 AGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKIL 597
                                              VI+YK+I++VFFVD+IP +PSGKIL
Sbjct: 545 -----------------------------------VIFYKRIKRVFFVDAIPKSPSGKIL 531

BLAST of Carg08355 vs. Swiss-Prot
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 3.0e-196
Identity = 358/605 (59.17%), Postives = 433/605 (71.57%), Query Frame = 0

Query: 9   QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVAT 68
           +D IFRSKLPDIYIP +LPLH+YCFE++S+F  RPCLIN AT +I TYA V+L +RRV +
Sbjct: 12  KDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGS 71

Query: 69  GLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKV 128
           GLSK+G+ QGD IM+LL NSPEFVFAFLGAS+ G+IST ANP +   E+ KQA  +  K+
Sbjct: 72  GLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNAKL 131

Query: 129 IITQAEFVEKVREFAVENGVKIICTDS-----PPVGCLRFSEILEADENEIPAVKINSND 188
           IITQ  +V+KV+++A ENGVKII  D+          L FSE+  ADENE+P V+I+ + 
Sbjct: 132 IITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISPDG 191

Query: 189 VVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLN 248
           VVALP+SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+ ++S+DVL+C+LPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYSLN 251

Query: 249 SVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSI 308
           SVLLCGLR G+ ILI+QK+++   L LIQ +K TI PFVPPIVL  AK+  +  YDLSS+
Sbjct: 252 SVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLSSV 311

Query: 309 RIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGT 368
           R VMSGAAP+GK LED V+A+ PNA LGQGYGMTEAGPVL+MCL FAKEPF+ KSGACGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 369 VVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDLG 428
           VVRNAEMKI+ PETG SLPRNHP            GYLN+ EAT +TIDK GWLHTGD+G
Sbjct: 372 VVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGDIG 431

Query: 429 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAF 488
           +IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P +S AAVVPM+DEAAGE+P AF
Sbjct: 432 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPVAF 491

Query: 489 VVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSS 548
           VV+S G  I+ED+IK++IS Q                                       
Sbjct: 492 VVKSNGHNITEDEIKQFISKQ--------------------------------------- 540

Query: 549 ILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGK 597
                                                VI+YK+I +VFFV++IP APSGK
Sbjct: 552 -------------------------------------VIFYKRINRVFFVEAIPKAPSGK 540

BLAST of Carg08355 vs. TrEMBL
Match: tr|A0A1S3BSW8|A0A1S3BSW8_CUCME (4-coumarate--CoA ligase 2-like OS=Cucumis melo OX=3656 GN=LOC103493360 PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 2.8e-252
Identity = 460/611 (75.29%), Postives = 489/611 (80.03%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           MD +  N QDFIF+SKLPDIYIPNHLPLHTYCFEHL+QF HRPCLIN  TG+IHTYA VD
Sbjct: 1   MDHEQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEVD 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           LAARR A GLSKIG+GQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQ
Sbjct: 61  LAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPA 180
           A  AR KVIITQ EFVEKV EFAVENGVKI+CTD    S   GCLRFSE++EADEN+IPA
Sbjct: 121 AVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIPA 180

Query: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPL 240
           VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++NS DV+IC+LPL
Sbjct: 181 VKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLPL 240

Query: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIH 300
           FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQ HKATIAPFVPPIVLDFAKNPDIH
Sbjct: 241 FHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDIH 300

Query: 301 RYDLSSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEF 360
            Y+LSSIRIVMSGAAPMGK LEDTVKARLPNA LGQGYGMTEAGPVLSMCLGFAKEPF  
Sbjct: 301 NYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFNV 360

Query: 361 KSGACGTVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGW 420
           KSGACGTVVRNAEMKIIHP++ +SLPRNHP            GYLNN+EATEKTIDK+GW
Sbjct: 361 KSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDGW 420

Query: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAA 480
           LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAA
Sbjct: 421 LHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAA 480

Query: 481 GEIPAAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPI 540
           GEIP AFVVRSKGSKISE+DIK YISDQ                                
Sbjct: 481 GEIPVAFVVRSKGSKISENDIKNYISDQ-------------------------------- 535

Query: 541 HPFSGSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSI 596
                                                       VIYYK+IRKVFFVDSI
Sbjct: 541 --------------------------------------------VIYYKRIRKVFFVDSI 535

BLAST of Carg08355 vs. TrEMBL
Match: tr|A0A0A0LM95|A0A0A0LM95_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 1.1e-248
Identity = 455/605 (75.21%), Postives = 487/605 (80.50%), Query Frame = 0

Query: 7   NAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRV 66
           N +DFIF+SKLPDIYIPNHLPLHTYCFEHLSQF HRPCLIN  TG+IHTYA V+LAARR 
Sbjct: 8   NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRF 67

Query: 67  ATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARP 126
           A GLSKIGVGQGDVIML+LQNSPEFVFAFLGAS+AGAI+TTANPLYKP EI KQA  A+ 
Sbjct: 68  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKV 127

Query: 127 KVIITQAEFVEKVREFAVENGVKIICTD----SPPVGCLRFSEILEADENEIPAVKINSN 186
           KVIITQ EFVEKV EFAVE+GVKI+CTD    S   GCL+FSE++EADENEIPAVKINS+
Sbjct: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSS 187

Query: 187 DVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSL 246
           DVVALPFSSGTTGVPKGVMLTHKSLVTS+AQQVDGENPNV++N  DV+IC+LPLFHIYSL
Sbjct: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247

Query: 247 NSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 306
           NSVLLCGLRVGAAILILQKYD+SSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS
Sbjct: 248 NSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 307

Query: 307 IRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACG 366
           IRIVMSGAAPMGK LEDTVKA+LPNA LGQGYGMTEAGPVLSMCLGFAKE F+ KSGACG
Sbjct: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367

Query: 367 TVVRNAEMKIIHPETGVSLPRNHP------------GYLNNKEATEKTIDKNGWLHTGDL 426
           TVVRNAEMKIIHP+T +SLPRN+P            GYLNNKEATEKTIDK+GWLHTGDL
Sbjct: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDL 427

Query: 427 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAA 486
           G+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPN++SAAV+PMEDEAAGEIP A
Sbjct: 428 GYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVA 487

Query: 487 FVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGS 546
           FVVRSKGSKISE+DIK YISDQ                                      
Sbjct: 488 FVVRSKGSKISENDIKNYISDQ-------------------------------------- 536

Query: 547 SILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSG 596
                                                 VIYYKKIRKVFFVDSIP+APSG
Sbjct: 548 --------------------------------------VIYYKKIRKVFFVDSIPMAPSG 536

BLAST of Carg08355 vs. TrEMBL
Match: tr|A0A067KVV8|A0A067KVV8_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_08679 PE=4 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.2e-215
Identity = 390/618 (63.11%), Postives = 461/618 (74.60%), Query Frame = 0

Query: 1   MDSQLTNAQDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVD 60
           M+++L N +DFIFRSKLPDIYIPNHLPLHTYCFE++SQF   PCLIN ATG+I+TYA V+
Sbjct: 1   METKLDNQEDFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNPCLINGATGEIYTYADVE 60

Query: 61  LAARRVATGLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQ 120
           L +R+VA GL K+G+ QGDVIMLLLQNSPEFVFAFLGAS+ GAISTTANP Y PAEIKKQ
Sbjct: 61  LTSRKVAAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIKKQ 120

Query: 121 AATARPKVIITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKIN 180
              ++ +++ITQA + EKV++FA ++ +K++  DSPP  CL FSE+   DEN+IP VKIN
Sbjct: 121 LTASKAQLVITQAVYAEKVKQFAKDSDIKVMTIDSPPEDCLHFSELTSCDENDIPTVKIN 180

Query: 181 SNDVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIY 240
           S+DVVALP+SSGTTG PKGVMLTHK LVTS+AQQVDGENPN+  +  DV++CLLPLFHIY
Sbjct: 181 SDDVVALPYSSGTTGFPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIY 240

Query: 241 SLNSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDL 300
           SLNSVLLCGLRVGAAILI+QK++++SL+ L+  +K TIAPFVPPIVL  AK+P + +YDL
Sbjct: 241 SLNSVLLCGLRVGAAILIMQKFEINSLMELVTKYKVTIAPFVPPIVLAIAKSPVVDKYDL 300

Query: 301 SSIRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGA 360
           SSIR VMSGAAPMGK LED V+A+LPNA LGQGYGMTEAGPVLSMCL FAKEPFE KSGA
Sbjct: 301 SSIRTVMSGAAPMGKELEDAVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGA 360

Query: 361 CGTVVRNAEMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLHTG 420
           CGTVVRNAEMKI+ P+TG SLPRN              GYLN+ EATE+TIDK+GWLHTG
Sbjct: 361 CGTVVRNAEMKIVDPDTGNSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTG 420

Query: 421 DLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIP 480
           D+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEA+L ++PN+S AAVVPM+DEAAGEIP
Sbjct: 421 DVGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIP 480

Query: 481 AAFVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFS 540
            AFVVRS GSKI+ED+IK+YIS Q                                    
Sbjct: 481 VAFVVRSNGSKITEDEIKQYISKQ------------------------------------ 540

Query: 541 GSSILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAP 600
                                                   VI+YK+I KVFF D+IP AP
Sbjct: 541 ----------------------------------------VIFYKRISKVFFTDAIPKAP 541

Query: 601 SGKILRKDRHQIQLPSGP 607
           SGKILRKD  + +L  GP
Sbjct: 601 SGKILRKDL-RARLADGP 541

BLAST of Carg08355 vs. TrEMBL
Match: tr|B9T4L7|B9T4L7_RICCO (AMP dependent CoA ligase, putative OS=Ricinus communis OX=3988 GN=RCOM_0246200 PE=4 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 2.9e-212
Identity = 388/606 (64.03%), Postives = 453/606 (74.75%), Query Frame = 0

Query: 9   QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVAT 68
           Q+FIFRSKLPDIYIPNHLPLHTYCFE++SQF    CLIN ATG ++TYA V+L +R+VA 
Sbjct: 17  QEFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNACLINGATGDVYTYADVELTSRKVAA 76

Query: 69  GLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKV 128
           GL K+G+ Q +VIM+LLQNSPEFVFAFLGAS  GAISTTANP Y PAEIKKQA+ +  K+
Sbjct: 77  GLHKLGIKQNEVIMILLQNSPEFVFAFLGASVLGAISTTANPFYTPAEIKKQASASNAKL 136

Query: 129 IITQAEFVEKVREFAVENGVKIICTDSPPVG------CLRFSEILEADENEIPAVKINSN 188
           IITQA +VEKV++FA ENG+KI+  DSPP        CL FSE+ +ADEN+IPAVKIN +
Sbjct: 137 IITQAAYVEKVKDFANENGIKIMTIDSPPPALDDCRDCLHFSELTKADENDIPAVKINPD 196

Query: 189 DVVALPFSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSL 248
           DVVALP+SSGTTG+PKGVMLTHKSLVTS+AQQVDGENPN+  +  DV++CLLPLFHIYSL
Sbjct: 197 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSL 256

Query: 249 NSVLLCGLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 308
           NSVLLCGLRVGAAILI+QK+++++L+ L+Q +K TIAPFVPPIVL  AK+P + +YDLSS
Sbjct: 257 NSVLLCGLRVGAAILIMQKFEITALMELVQKYKVTIAPFVPPIVLSIAKSPAVDKYDLSS 316

Query: 309 IRIVMSGAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACG 368
           IR VMSGAAPMGK LEDTV+A+LPNA LGQGYGMTEAGPVLSMCL FAKEPFE KSGACG
Sbjct: 317 IRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACG 376

Query: 369 TVVRNAEMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLHTGDL 428
           TVVRNAEMKI+ P+TG SL RN              GYLN+ EATE+TIDK GWLHTGD+
Sbjct: 377 TVVRNAEMKIVDPDTGKSLQRNQAGEICIRGSQIMKGYLNDPEATERTIDKEGWLHTGDV 436

Query: 429 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAA 488
           G+ID DDE+FIVDRLKELIKY G+QVAPAELEA+L ++P++S AAVVPM+DEAAGEIPAA
Sbjct: 437 GYIDGDDELFIVDRLKELIKYNGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGEIPAA 496

Query: 489 FVVRSKGSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGS 548
           FVVRS GSKI+EDD+++YIS Q                                      
Sbjct: 497 FVVRSNGSKITEDDVQQYISKQ-------------------------------------- 546

Query: 549 SILAGTPLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSG 597
                                                 VIYYK+IR+VFF DSIP APSG
Sbjct: 557 --------------------------------------VIYYKRIRRVFFTDSIPKAPSG 546

BLAST of Carg08355 vs. TrEMBL
Match: tr|J7FW91|J7FW91_COFAR (4-hydroxycinnamoyl-CoA ligase 3 OS=Coffea arabica OX=13443 GN=4CL3 PE=2 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 3.7e-212
Identity = 381/600 (63.50%), Postives = 451/600 (75.17%), Query Frame = 0

Query: 9   QDFIFRSKLPDIYIPNHLPLHTYCFEHLSQFHHRPCLINAATGQIHTYAAVDLAARRVAT 68
           Q+F+FRSKLPDIYIPNHLPLH+YCFE++ +F +RPCLIN  TG I+TYA V+L AR+VA 
Sbjct: 8   QEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELIARKVAA 67

Query: 69  GLSKIGVGQGDVIMLLLQNSPEFVFAFLGASYAGAISTTANPLYKPAEIKKQAATARPKV 128
           GL KIG+ QG+VIMLLLQNSPEF FA LGASY GA+STTANP YKPAEI+KQA  ++ K+
Sbjct: 68  GLDKIGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQARASKAKL 127

Query: 129 IITQAEFVEKVREFAVENGVKIICTDSPPVGCLRFSEILEADENEIPAVKINSNDVVALP 188
           IITQ+ +VEKV +FA EN VK++CTD+PP GCL FSE+  ADE  IPAVKIN ND VALP
Sbjct: 128 IITQSCYVEKVMDFAKENNVKVMCTDAPPEGCLHFSELSSADEKVIPAVKINPNDAVALP 187

Query: 189 FSSGTTGVPKGVMLTHKSLVTSIAQQVDGENPNVDLNSNDVLICLLPLFHIYSLNSVLLC 248
           +SSGTTG+PKGVMLTHK LVTS+AQQVDGENPN+  +  DV++C+LPLFHIYSLNSVLLC
Sbjct: 188 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFHIYSLNSVLLC 247

Query: 249 GLRVGAAILILQKYDMSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRIVMS 308
           GLRVGAAILI+QK+++++L+ L+Q +K TIAPFVPPIVL+ AK+P + +YDLSSIR+VMS
Sbjct: 248 GLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKYDLSSIRMVMS 307

Query: 309 GAAPMGKALEDTVKARLPNATLGQGYGMTEAGPVLSMCLGFAKEPFEFKSGACGTVVRNA 368
           GAAPMGK LEDTV+A+LP A LGQGYGMTEAGP+LSMCL FAKEPF+ KSGACGTVVRNA
Sbjct: 308 GAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKSGACGTVVRNA 367

Query: 369 EMKIIHPETGVSLPRNH------------PGYLNNKEATEKTIDKNGWLHTGDLGFIDDD 428
           EMKI+ PET +SLPRN              GYLN+ EATE TIDK GWLHTGD+G+IDDD
Sbjct: 368 EMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLHTGDIGYIDDD 427

Query: 429 DEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNVSSAAVVPMEDEAAGEIPAAFVVRSK 488
           DEIFIVDRLKELIKYKG+QVAPAELEA+L S+P +S AAVV M+DEAAGE+P AFVVR+ 
Sbjct: 428 DEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGEVPVAFVVRAS 487

Query: 489 GSKISEDDIKKYISDQKVLLSSQSTWDFTVSASWVTHSLLQSVWDPPIHPFSGSSILAGT 548
           GSKISED+IK++IS+Q                                            
Sbjct: 488 GSKISEDEIKQFISNQ-------------------------------------------- 531

Query: 549 PLRVHPFQGLASLLAYYPMFGFDIIYNEPSPPVIYYKKIRKVFFVDSIPVAPSGKILRKD 597
                                           VI+YK+I +VFF+D IP APSGKILRKD
Sbjct: 548 --------------------------------VIFYKRIHRVFFMDKIPKAPSGKILRKD 531