CsaV3_1G031500 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_1G031500 vs. NCBI nr
Match: XP_004146311.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_1G423020 [Cucumis sativus])

HSP 1 Score: 1058.5 bits (2736), Expect = 7.6e-306
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180
           AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180

Query: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240
           VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL
Sbjct: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240

Query: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300
           FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD
Sbjct: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300

Query: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360
           HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI
Sbjct: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360

Query: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420
           KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW
Sbjct: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420

Query: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480
           LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA
Sbjct: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480

Query: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540
           GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL
Sbjct: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540

Query: 541 LAASIS 547
           LAASIS
Sbjct: 541 LAASIS 546

BLAST of CsaV3_1G031500 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 973.8 bits (2516), Expect = 2.5e-280
Identity = 504/550 (91.64%), Postives = 523/550 (95.09%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFL ASYLGAAATMANPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180
           AVSSGAKVIITQ+AFAEKVK LSQ +EMIIKVIFID          +FSSLI+DVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDXXXXXTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240
           LEMGDVKISP+DVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMC+LRVGAAIL+VQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASI 546
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of CsaV3_1G031500 vs. NCBI nr
Match: XP_023515445.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 3.1e-243
Identity = 447/549 (81.42%), Postives = 481/549 (87.61%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAAD  SPEFIFRSKLP++ I  HLPLHTYCFE ISEFKH PCLINAATG IYTYGEV 
Sbjct: 1   MAAAD-PSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
            T+RRVAAGLHKLGIGKGDVIMLLLQNTP+FV AFLGAS+  A ATMANPFF  +EI KQ
Sbjct: 61  VTARRVAAGLHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIGKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQ----FSSLIEDVAKEEEL 180
           A SSGAK+IITQAAFAEKVK L QEN+ I+KV+FID   PP+    FSSL  D AKEE  
Sbjct: 121 AASSGAKLIITQAAFAEKVKMLPQENQGILKVVFID--SPPEGGCHFSSL-TDEAKEE-- 180

Query: 181 EMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 240
           EM DVK SP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPH HIRS+DVVLC
Sbjct: 181 EMADVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLC 240

Query: 241 LLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 300
           +LPLFHIYSL+SI+M +LRVGAAIL+VQKFD+NSLV LV KY+VT A  VPPIVLA+ KS
Sbjct: 241 VLPLFHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKS 300

Query: 301 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 360
           P VDH DMSS+RIVLSGAAPLGKNLEDAFRAKLPH ILGQGYGMTE+GSV+TMSLA  KE
Sbjct: 301 PVVDHSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAKE 360

Query: 361 GFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 420
            F IKSG CGT+MRN+EMKI+N  TG SL +NQ GEI ++S QMMKGYLNDE+ATK IID
Sbjct: 361 RFAIKSGACGTLMRNAEMKIVNPDTGVSLSKNQPGEIYVKSPQMMKGYLNDEEATKGIID 420

Query: 421 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 480
           E GWLHTGDIG VDDDDE+FIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AVIPM 
Sbjct: 421 EHGWLHTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMK 480

Query: 481 DEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540
           DEVAGEVPVAFIVR DGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ
Sbjct: 481 DEVAGEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540

Query: 541 LRALLAASI 546
           LRALL ASI
Sbjct: 541 LRALLPASI 543

BLAST of CsaV3_1G031500 vs. NCBI nr
Match: XP_022987367.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 848.6 bits (2191), Expect = 1.2e-242
Identity = 448/549 (81.60%), Postives = 480/549 (87.43%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAAD  SPEFIFRSKLP++ I  HLPLHTYCFE ISEFKH PCLINAATG IYTYGEV 
Sbjct: 1   MAAAD-PSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
            T+RRVAAGLHKLGIGKGDVIMLLLQNTP+F  AFLGAS+  A ATMANPFF  +EIAKQ
Sbjct: 61  VTARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQ----FSSLIEDVAKEEEL 180
           A SSGAK+IITQAAFAEKVKNL QEN+ I+KVIFID   PP+    FSSL  D AKEEEL
Sbjct: 121 AASSGAKLIITQAAFAEKVKNLPQENQGILKVIFID--SPPEGGCHFSSL-TDEAKEEEL 180

Query: 181 EMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 240
              DVK SP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPH HIRS+DVVLC
Sbjct: 181 T--DVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLC 240

Query: 241 LLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 300
           +LPLFHIYSL+SI+M +LRVGAAILLVQKFD+NSLV LV KY+VT A  VPPIVLA+ KS
Sbjct: 241 VLPLFHIYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKS 300

Query: 301 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 360
           P VD  DMSS+RIVLSGAAPLGKNLEDAFRAK PH ILGQGYGMTE+GSV+TMSLA  KE
Sbjct: 301 PVVDCSDMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKE 360

Query: 361 GFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 420
            F IKSG CGT+MRN+EMKI+N +TG SL RNQ GEI +RS QMMKGYLNDE+ATK IID
Sbjct: 361 RFAIKSGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIID 420

Query: 421 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 480
            DGWLHTGDIG VDDDDE+FIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AVIPM 
Sbjct: 421 GDGWLHTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMK 480

Query: 481 DEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540
           DEVAGE PVAFIVR DGSNITEDEIK++ISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ
Sbjct: 481 DEVAGEAPVAFIVRSDGSNITEDEIKEYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540

Query: 541 LRALLAASI 546
           LRALL ASI
Sbjct: 541 LRALLPASI 543

BLAST of CsaV3_1G031500 vs. NCBI nr
Match: XP_022921335.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 847.8 bits (2189), Expect = 2.0e-242
Identity = 445/549 (81.06%), Postives = 481/549 (87.61%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAAD SSPEFIFRSKLP++ I  HLPLHTYCFE IS+FKH PCLINAATG IYTYGEV 
Sbjct: 1   MAAAD-SSPEFIFRSKLPDLTIPNHLPLHTYCFERISKFKHNPCLINAATGDIYTYGEVH 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
            T+RRVAAG HKLGIGKGDVIMLLLQNTP+FV AFLGAS+  A ATMANPFF  +EIAKQ
Sbjct: 61  VTARRVAAGFHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQ----FSSLIEDVAKEEEL 180
           A SSGAK+IITQAAFAEKVK L QEN+ I+KVIFID   PP+    F+SL  D AKEE  
Sbjct: 121 AASSGAKLIITQAAFAEKVKKLPQENQGILKVIFID--SPPEGGCHFTSL-TDEAKEE-- 180

Query: 181 EMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLC 240
           EM DVK SP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPH HIRS+DVVLC
Sbjct: 181 EMADVKFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLC 240

Query: 241 LLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKS 300
           +LPLFHIYSL+SI+M +LRVGAAIL+VQKFD+NSLV LV KY+VT A  VPPIVLA+ KS
Sbjct: 241 VLPLFHIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKS 300

Query: 301 PAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKE 360
           P VDH DMSS+RIVLSGAAPLGKNLEDAFRAKLPH IL QGYGMTE+GSV+TMSLA  +E
Sbjct: 301 PVVDHSDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILAQGYGMTESGSVLTMSLALARE 360

Query: 361 GFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIID 420
            F IKSG CGT+MRN+EMKI+N + G SL RNQ GEI +RS QMMKGYLNDE+ATK +ID
Sbjct: 361 KFAIKSGACGTLMRNAEMKIVNPENGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGMID 420

Query: 421 EDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMN 480
           E GWLHTGD+G VDDDDELFIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AVIPM 
Sbjct: 421 ESGWLHTGDMGLVDDDDELFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMK 480

Query: 481 DEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540
           DEVAGEVPVAFIVR DGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ
Sbjct: 481 DEVAGEVPVAFIVRSDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRRQ 540

Query: 541 LRALLAASI 546
           LRALL ASI
Sbjct: 541 LRALLPASI 543

BLAST of CsaV3_1G031500 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 695.7 bits (1794), Expect = 2.3e-200
Identity = 349/537 (64.99%), Postives = 428/537 (79.70%), Query Frame = 0

Query: 8   SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVA 67
           S + IFRS+LP+I I  HLPLH Y FENISEF  +PCLIN  TG +YTY +V  TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAK 127
           AGLH LG+ + DV+M+LL N+PE VL FL AS++GA  T ANPFF  +EI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 VIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDV--KISP 187
           +I+TQ+ + +K+KNL  +  +I+     D +  P+      ++ + EE  +  +  KISP
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTT---DSDAIPENCLRFSELTQSEEPRVDSIPEKISP 197

Query: 188 EDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYS 247
           EDVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+   DDV+LC+LP+FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 248 LNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMS 307
           LNSIM+C+LRVGA IL++ KF++  L+E + + KVT A  VPPIVLAIAKSP  + +D+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 308 SLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGC 367
           S+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL F KE F +KSG C
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 368 GTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGD 427
           GT++RN+EMKIL+  TG+SLPRN+ GEICIR +Q+MKGYLND  AT + ID+DGWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 428 IGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPV 487
           +GF+DDDDELFIVDRLKELIKYKGFQVAPAELE+LLI H  I D AV+ M +E AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 488 AFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 543
           AF+VR   SNI+EDEIKQF+SKQVVFYKRIN+VFF DSIPK+PSGKILR+ LRA LA
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of CsaV3_1G031500 vs. TAIR10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1)

HSP 1 Score: 694.9 bits (1792), Expect = 4.0e-200
Identity = 349/543 (64.27%), Postives = 430/543 (79.19%), Query Frame = 0

Query: 7   SSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRV 66
           ++ + IFRSKLP+I I  HL LH Y F+NISEF  +PCLIN  TGH+YTY +V   SR++
Sbjct: 19  NNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQI 78

Query: 67  AAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGA 126
           AA  HKLG+ + DV+MLLL N PEFVL+FL AS+ GA AT ANPFF  +EIAKQA +S  
Sbjct: 79  AANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNT 138

Query: 127 KVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-------QFSSLIEDVAKEEELEMG 186
           K+IIT+A + +K+K L  ++ ++I  + IDDN+         +F+ L +   +  E+ + 
Sbjct: 139 KLIITEARYVDKIKPLQNDDGVVI--VCIDDNESVPIPEGCLRFTELTQSTTEASEV-ID 198

Query: 187 DVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLP 246
            V+ISP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+  SDDV+LC+LP
Sbjct: 199 SVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLP 258

Query: 247 LFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAV 306
           +FHIY+LNSIM+C LRVGAAIL++ KF++N L+EL+ + KVT AP VPPIVLAIAKS   
Sbjct: 259 MFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSET 318

Query: 307 DHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFG 366
           + +D+SS+R+V SGAAPLGK LEDA  AK P+  LGQGYGMTEAG V+ MSL F KE F 
Sbjct: 319 EKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 378

Query: 367 IKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDG 426
           +KSG CGT++RN+EMKI++  TG+SL RNQ GEICIR  Q+MKGYLN+  AT   ID+DG
Sbjct: 379 VKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDG 438

Query: 427 WLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEV 486
           WLHTGDIG +DDDDELFIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ M +E 
Sbjct: 439 WLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEA 498

Query: 487 AGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRA 543
           AGEVPVAF+V+   S ++ED++KQF+SKQVVFYKRIN+VFF +SIPK+PSGKILR+ LRA
Sbjct: 499 AGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRA 558

BLAST of CsaV3_1G031500 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 656.0 bits (1691), Expect = 2.1e-188
Identity = 325/530 (61.32%), Postives = 423/530 (79.81%), Query Frame = 0

Query: 12  IFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVAAGLH 71
           IFRSKLP+I I  HLPLHTYCFE +S    +PCLI  +TG  YTYGE     RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAKVIIT 131
           KLGI KGDVIM+LLQN+ EFV +F+GAS +GA +T ANPF+   E+ KQ  SSGAK+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPEDVVAL 191
            + + +K+KNL +   +I       +N  P FS+LI D   E       V I  +D  AL
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLP-FSTLITD--DETNPFQETVDIGGDDAAAL 213

Query: 192 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLNSIMM 251
           P+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NP+L+++S+DV+LC+LPLFHIYSLNS+++
Sbjct: 214 PFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLL 273

Query: 252 CALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSLRIVL 311
            +LR GA +LL+ KF++ +L++L+ +++VT A  VPP+V+A+AK+P V+ +D+SS+R VL
Sbjct: 274 NSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVL 333

Query: 312 SGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGTIMRN 371
           SGAAPLGK L+D+ R +LP  ILGQGYGMTEAG V++MSL F KE    KSG CGT++RN
Sbjct: 334 SGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRN 393

Query: 372 SEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIGFVDD 431
           +E+K+++L+T  SL  NQ GEICIR  Q+MK YLND +AT A IDE+GWLHTGDIG+VD+
Sbjct: 394 AELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDE 453

Query: 432 DDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAFIVRF 491
           DDE+FIVDRLKE+IK+KGFQV PAELE+LLI+H  IADAAV+P NDEVAGEVPVAF+VR 
Sbjct: 454 DDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRS 513

Query: 492 DGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALL 542
           +G++ITE+++K++++KQVVFYKR+++VFFV SIPKSPSGKILR+ L+A L
Sbjct: 514 NGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CsaV3_1G031500 vs. TAIR10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5)

HSP 1 Score: 633.6 bits (1633), Expect = 1.1e-181
Identity = 323/549 (58.83%), Postives = 414/549 (75.41%), Query Frame = 0

Query: 8   SPEFIFRSKLPEIPISTHLPLHTYCFENIS----EFKHRPCLINAATGHIYTYGEVQATS 67
           S +FIFRSKLP+I I  HLPL  Y F+  S          C+I+ ATG I TY +VQ   
Sbjct: 24  SHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQTNM 83

Query: 68  RRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVS 127
           RR+AAG+H+LGI  GDV+MLLL N+PEF L+FL  +YLGA +T ANPF+ Q EIAKQA +
Sbjct: 84  RRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQAKA 143

Query: 128 SGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSS------LIEDVAKEEELE 187
           S AK+IIT+    +K+ NL  +N+ ++ V   DD D    SS         ++ + +E E
Sbjct: 144 SAAKMIITKKCLVDKLTNL--KNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETE 203

Query: 188 MGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCL 247
           +   KISPED VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENP+L+  ++DV+LC 
Sbjct: 204 LLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCF 263

Query: 248 LPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSP 307
           LP+FHIY+L+++M+ A+R GAA+L+V +F++N ++EL+ +YKVT  P  PP+VLA  KSP
Sbjct: 264 LPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSP 323

Query: 308 AVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEG 367
             + +D+SS+RI+LSGAA L K LEDA R K P+ I GQGYGMTE+G+V   SLAF K  
Sbjct: 324 ETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNP 383

Query: 368 FGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDE 427
           F  KSG CGT++RN+EMK+++ +TG SLPRN++GEIC+R  Q+MKGYLND +AT   ID+
Sbjct: 384 FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDK 443

Query: 428 DGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMND 487
           DGWLHTGDIGFVDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I DAAV+ M D
Sbjct: 444 DGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKD 503

Query: 488 EVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQL 547
           EVA EVPVAF+ R  GS +TED++K +++KQVV YKRI  VFF++ IPK+ SGKILR+ L
Sbjct: 504 EVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDL 563

BLAST of CsaV3_1G031500 vs. TAIR10
Match: AT1G62940.1 (acyl-CoA synthetase 5)

HSP 1 Score: 395.6 bits (1015), Expect = 5.0e-110
Identity = 222/535 (41.50%), Postives = 329/535 (61.50%), Query Frame = 0

Query: 10  EFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVAAG 69
           E+IFRS  P +PI   L L  +  + + E+      + A TG   TYG+V   ++R+A  
Sbjct: 10  EYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRLAKA 69

Query: 70  LHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAKVI 129
           L  LG+ KG V++++L N  E+ +  LG    G   + ANP  + SEI KQ  +SGA+ I
Sbjct: 70  LTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGARGI 129

Query: 130 ITQAAFAEKVKNLSQENEMIIKVIFIDD---NDPPQFSSLIEDVAKEEELEMGDVKISPE 189
           IT A   EKVK+L       + VI + +        +  L+E  A ++  +  + +I   
Sbjct: 130 ITDATNYEKVKSLG------LPVIVLGEEKIEGAVNWKDLLE--AGDKCGDTDNEEILQT 189

Query: 190 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSL 249
           D+ ALP+SSGTTGL KGVMLTH+ L+ ++   + G    +      V L L+P FHIY +
Sbjct: 190 DLCALPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEM--IGQIVTLGLIPFFHIYGI 249

Query: 250 NSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSS 309
             I    ++    ++ + ++D+   +  +  ++V+FAP VPPI+L + K+P VD FD+S 
Sbjct: 250 VGICCATMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSK 309

Query: 310 LRI--VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI-KSG 369
           L++  V++ AAPL   L  AF AK P+V + + YG+TE  S +T++    ++G GI K  
Sbjct: 310 LKLQSVMTAAAPLAPELLTAFEAKFPNVQVQEAYGLTE-HSCITLTHGDPEKGQGIAKRN 369

Query: 370 GCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHT 429
             G I+ N E+K ++  TG SLP+N +GE+C+RS  +M+GY  +++ T   IDE GWLHT
Sbjct: 370 SVGFILPNLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHT 429

Query: 430 GDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEV 489
           GDIG++DDD ++FIVDR+KELIKYKGFQVAPAELEA+L++H  + D AV+P+ DE AGE+
Sbjct: 430 GDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEI 489

Query: 490 PVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLR 539
           P A +V    +   E++I  F++  V  YK++  V FVDSIPKS SGKI+RR LR
Sbjct: 490 PAACVVINPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLR 533

BLAST of CsaV3_1G031500 vs. Swiss-Prot
Match: sp|P31684|4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.1e-212
Identity = 374/539 (69.39%), Postives = 447/539 (82.93%), Query Frame = 0

Query: 8   SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVA 67
           S + IFRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A   IYTY EV+ TSR+VA
Sbjct: 10  SGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 69

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N PEFV AF+GASYLGA +TMANP F  +E+ KQA +S AK
Sbjct: 70  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 129

Query: 128 VIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPED 187
           ++ITQA FA KVK+ + EN+  +KVI +D    P+      ++ + +E E+ DVKI P+D
Sbjct: 130 IVITQACFAGKVKDYAIEND--LKVICVD--SVPEGCVHFSELIQSDEHEIPDVKIQPDD 189

Query: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLN 247
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ SDDV++C+LPLFHIYSLN
Sbjct: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLN 249

Query: 248 SIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSL 307
           S+++CALRVGAAIL++QKFD+   +EL+ K+KVT  PFVPPIVLAIAKSP VD++D+SS+
Sbjct: 250 SVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSV 309

Query: 308 RIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGT 367
           R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAF KE F IKSG CGT
Sbjct: 310 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 369

Query: 368 IMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIG 427
           ++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   I+++GWLHTGDIG
Sbjct: 370 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIG 429

Query: 428 FVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAF 487
           F+DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE AGEVPVAF
Sbjct: 430 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAF 489

Query: 488 IVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASIS 547
           +VR +GS ITEDE+K FISKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA LAA IS
Sbjct: 490 VVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGIS 544

BLAST of CsaV3_1G031500 vs. Swiss-Prot
Match: sp|P31685|4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 5.7e-212
Identity = 373/539 (69.20%), Postives = 446/539 (82.75%), Query Frame = 0

Query: 8   SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVA 67
           S + IFRSKLP+I I  HLPLH+YCFEN+SEF  RPCLI+ A   IYTY EV+ TSR+VA
Sbjct: 10  SGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 69

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N PEFV AF+GASYLGA +TMANP F  +E+ KQA +S AK
Sbjct: 70  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 129

Query: 128 VIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPED 187
           ++ITQA FA KVK+ + EN+  +KVI +D    P+      ++ + +E E+ DVKI P+D
Sbjct: 130 IVITQACFAGKVKDYAIEND--LKVICVD--SAPEGCVHFSELIQSDEHEIPDVKIQPDD 189

Query: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLN 247
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ SDDV++C+LPLFHIYSLN
Sbjct: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLN 249

Query: 248 SIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSL 307
           S+++CALRVGAAIL++QKFD+   +EL+ K+KVT  PFVPPIVLAIAKSP V ++D+SS+
Sbjct: 250 SVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSV 309

Query: 308 RIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGT 367
           R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAF KE F IKSG CGT
Sbjct: 310 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 369

Query: 368 IMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIG 427
           ++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   I+++GWLHTGDIG
Sbjct: 370 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIG 429

Query: 428 FVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAF 487
           F+DDDDELFIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE AGEVPVAF
Sbjct: 430 FIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAF 489

Query: 488 IVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASIS 547
           +VR +GS ITEDE+K FISKQV+FYKRI RVFFV+++PKSPSGKILR+ LRA LAA IS
Sbjct: 490 VVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAGIS 544

BLAST of CsaV3_1G031500 vs. Swiss-Prot
Match: sp|O24146|4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 2.2e-211
Identity = 369/541 (68.21%), Postives = 443/541 (81.89%), Query Frame = 0

Query: 5   DASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSR 64
           D    + IFRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A   IYTY +V+  SR
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63

Query: 65  RVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSS 124
           +VAAGLHK GI   D IM+LL N+PEFV AF+GASYLGA +TMANP F  +E+ KQA +S
Sbjct: 64  KVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKAS 123

Query: 125 GAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKIS 184
            AK+I+TQA    KVK+ + EN+  +K+I ID    P+       + +  E ++ +V+I 
Sbjct: 124 SAKIIVTQACHVNKVKDYAFEND--VKIICID--SAPEGCLHFSVLTQANEHDIPEVEIQ 183

Query: 185 PEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIY 244
           P+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L+I S+DV+LC+LPLFHIY
Sbjct: 184 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 243

Query: 245 SLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDM 304
           SLNS+++C LRVGAAIL++QKFD+ S +EL+ +YKVT  PFVPPIVLAIAKSP VD +D+
Sbjct: 244 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 303

Query: 305 SSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGG 364
           SS+R V+SGAAPLGK LED  RAK P+  LGQGYGMTEAG V+ M LAF KE F IKSG 
Sbjct: 304 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 363

Query: 365 CGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTG 424
           CGT++RN+EMKI++ +TG SLPRNQ+GEICIR  Q+MKGYLND +AT   ID++GWL+TG
Sbjct: 364 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 423

Query: 425 DIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVP 484
           DIG++DDDDELFIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAV+PM DE AGEVP
Sbjct: 424 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 483

Query: 485 VAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAAS 544
           VAF+VR +GS ITEDE+K FISKQV+FYKRI RVFFVD+IPKSPSGKILR+ LRA LAA 
Sbjct: 484 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 540

Query: 545 I 546
           +
Sbjct: 544 L 540

BLAST of CsaV3_1G031500 vs. Swiss-Prot
Match: sp|O24145|4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 1.4e-210
Identity = 367/538 (68.22%), Postives = 443/538 (82.34%), Query Frame = 0

Query: 8   SPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVA 67
           S + IFRSKLP+I I  HLPLH+YCFENISEF  RPCLIN A   IYTY EV+ T R+VA
Sbjct: 12  SGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVA 71

Query: 68  AGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAK 127
            GL+KLGI + D IM+LL N+PEFV AF+GASYLGA +TMANP F  +E+ KQA +S AK
Sbjct: 72  VGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 128 VIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPED 187
           +IITQ+ F  KVK+ + EN+  +KVI ID    P+      ++ + +E E+ +VKI P+D
Sbjct: 132 IIITQSCFVGKVKDYASEND--VKVICID--SAPEGCLHFSELTQSDEHEIPEVKIQPDD 191

Query: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLN 247
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +L++ S+DV++C+LPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 251

Query: 248 SIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSL 307
           SI++C LRVGAAIL++QKFD+   +EL+ KYKV+  PFVPPIVLAIAKSP VD +D+SS+
Sbjct: 252 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 311

Query: 308 RIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGT 367
           R V+SGAAPLGK LEDA R K P+  LGQGYGMTEAG V+ M LAF KE F IKSG CGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 368 IMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIG 427
           ++RN+EMKI++  TG SLPRNQ GEICIR  Q+MKGYLND +AT   ID++GWLHTGDIG
Sbjct: 372 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 431

Query: 428 FVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAF 487
           F+D+DDELFIVDRLKELIKYKGFQVAPAE+EALL++H +I+DAAV+PM DE AGEVPVAF
Sbjct: 432 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 491

Query: 488 IVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASI 546
           +VR +GS ITEDE+K FISKQV+FYKR+ RVFFV+++PKSPSGKILR+ LRA LAA +
Sbjct: 492 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGV 545

BLAST of CsaV3_1G031500 vs. Swiss-Prot
Match: sp|O24540|4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 6.5e-208
Identity = 364/545 (66.79%), Postives = 445/545 (81.65%), Query Frame = 0

Query: 3   AADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQAT 62
           A +    + IFRSKLP+I I  +LPLH+YCFENIS+F  RPCLIN AT  I+TY +V+  
Sbjct: 6   AIEEQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELI 65

Query: 63  SRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAV 122
           SRRV +GL KLGI +GD IM+LL N+PEFV AFLGAS++G+ +TMANPFF  +E+ KQA 
Sbjct: 66  SRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAK 125

Query: 123 SSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLI---EDVAKEEELEMG 182
           +S AK+IITQ  + +KVK+ + EN   +K+I ID       ++ I    ++   +E EM 
Sbjct: 126 ASNAKLIITQGCYVDKVKDYACENG--VKIISIDTTTTADDAANILHFSELTGADENEMP 185

Query: 183 DVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLP 242
            V+ISP+ VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L++ SDDV+LC+LP
Sbjct: 186 KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 245

Query: 243 LFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAV 302
           LFHIYSLNS+++C LR G+ IL++QKF++   +EL+ KYKVT  PFVPPIVLAIAKS  V
Sbjct: 246 LFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVV 305

Query: 303 DHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFG 362
           D++D+SS+R V+SGAAPLGK LEDA RAK P+  LGQGYGMTEAG V+ M LAF KE F 
Sbjct: 306 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 365

Query: 363 IKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDG 422
           IKSG CGT++RN+EMKI++ +TG SLPRN  GEICIR  Q+MKGYLND +AT   ID++G
Sbjct: 366 IKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEG 425

Query: 423 WLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEV 482
           WLHTGDIG++DDDDELFIVDRLKELIKYKGFQVAPAELEALL++H  I+DAAV+PM DE 
Sbjct: 426 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEA 485

Query: 483 AGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRA 542
           AGEVPVAF+V+ +G NITEDEIKQFISKQV+FYKRINRVFFV++IPK+PSGKILR+ LRA
Sbjct: 486 AGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRA 545

Query: 543 LLAAS 545
            LAA+
Sbjct: 546 RLAAA 548

BLAST of CsaV3_1G031500 vs. TrEMBL
Match: tr|A0A0A0LWT6|A0A0A0LWT6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 5.0e-306
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180
           AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGD 180

Query: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240
           VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL
Sbjct: 181 VKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPL 240

Query: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300
           FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD
Sbjct: 241 FHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVD 300

Query: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360
           HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI
Sbjct: 301 HFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGI 360

Query: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420
           KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW
Sbjct: 361 KSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGW 420

Query: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480
           LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA
Sbjct: 421 LHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVA 480

Query: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540
           GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL
Sbjct: 481 GEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRAL 540

Query: 541 LAASIS 547
           LAASIS
Sbjct: 541 LAASIS 546

BLAST of CsaV3_1G031500 vs. TrEMBL
Match: tr|A0A1S3BWP0|A0A1S3BWP0_CUCME (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 1.6e-280
Identity = 504/550 (91.64%), Postives = 523/550 (95.09%), Query Frame = 0

Query: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60
           MAAAD SSPEF+FRSKLPEIPISTHLPLHTYCFENISEFK RPCLINAATGH YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120
           ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFL ASYLGAAATMANPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180
           AVSSGAKVIITQ+AFAEKVK LSQ +EMIIKVIFID          +FSSLI+DVAKEEE
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDXXXXXTKGGYKFSSLIDDVAKEEE 180

Query: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240
           LEMGDVKISP+DVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300
           CLLPLFHIYSLNSIMMC+LRVGAAIL+VQKFD+NSL+ELV KYKVTFAP VPPIVLAIAK
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360
           SPAVDHFDMSSLR+VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420
           EGFGIKSGGCGTIMRNSEMKI+  QTG SLPRN+TGEICIRS QMMKGYLNDE+ATKAII
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480
           D+DGWLHTGDIG+VDDDDELFIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540
           NDEVAGEVPV FIVRFDGSNITEDEIKQ+ISKQVVFYKRINRVFFVDSIPKSPSGKILRR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQVVFYKRINRVFFVDSIPKSPSGKILRR 540

Query: 541 QLRALLAASI 546
           QLRALLAASI
Sbjct: 541 QLRALLAASI 550

BLAST of CsaV3_1G031500 vs. TrEMBL
Match: tr|A0A067DU39|A0A067DU39_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g009077mg PE=4 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 3.7e-216
Identity = 382/536 (71.27%), Postives = 454/536 (84.70%), Query Frame = 0

Query: 10  EFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVAAG 69
           EFIFRSKLP+I I  HLPLHTYCFENIS+F  RPC+IN  TG +YTY +V+ TSR+VAAG
Sbjct: 9   EFIFRSKLPDIYIPNHLPLHTYCFENISQFADRPCIINGPTGDVYTYADVELTSRKVAAG 68

Query: 70  LHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAKVI 129
           L KLG+ KGDVIMLLLQN P+FV AFLGASY+GA +T ANPF+   EIAKQA++S AK+I
Sbjct: 69  LSKLGVQKGDVIMLLLQNCPQFVFAFLGASYIGATSTTANPFYTPPEIAKQAIASKAKII 128

Query: 130 ITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSL--IEDVAKEEELEMGDVKISPED 189
           ITQA + +KV++LS+ +   I  I     DPP  + L   E + + +E E+  VKI+P+D
Sbjct: 129 ITQAVYNDKVRDLSEHHGARIVTI-----DPPPENCLHFSELITQADENEIPAVKINPDD 188

Query: 190 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLN 249
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+L++  +DV+LC+LPLFHIYSLN
Sbjct: 189 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYLHKEDVILCVLPLFHIYSLN 248

Query: 250 SIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSL 309
           S+++CALRVGAAIL++QKFD+  L+ELV KYKVT APFVPPIVLA+AKS  VD +DMSS+
Sbjct: 249 SVLLCALRVGAAILIMQKFDIVKLMELVQKYKVTVAPFVPPIVLAVAKSGDVDKYDMSSI 308

Query: 310 RIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGT 369
           R V+SGAAP+GK LEDA RAKLPH  LGQGYGMTEAG V++M LAF KE F IKSG CGT
Sbjct: 309 RTVMSGAAPMGKELEDAVRAKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGT 368

Query: 370 IMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIG 429
           ++RN+EMKI++  TG SLPRNQ GEICIR +Q+MKGYLND +AT+  ID++GWLHTGDIG
Sbjct: 369 VVRNAEMKIVDPDTGISLPRNQAGEICIRGAQIMKGYLNDREATETTIDKEGWLHTGDIG 428

Query: 430 FVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAF 489
           ++DDDDELFIVDRLKELIK+KGFQVAPAELEA+LI+H +I+DAAV+PM DE AGEVPVAF
Sbjct: 429 YIDDDDELFIVDRLKELIKFKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAF 488

Query: 490 IVRFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAA 544
           +VR +GS ITEDEIKQ+ISKQVVFYKRINRVFF DSIPK+PSGKILR+ LR  LAA
Sbjct: 489 VVRSNGSKITEDEIKQYISKQVVFYKRINRVFFTDSIPKAPSGKILRKDLRTKLAA 539

BLAST of CsaV3_1G031500 vs. TrEMBL
Match: tr|A0A1R3G3Z5|A0A1R3G3Z5_COCAP (AMP-dependent synthetase/ligase OS=Corchorus capsularis OX=210143 GN=CCACVL1_29084 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 6.3e-216
Identity = 380/536 (70.90%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 10  EFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVAAG 69
           +FIFRS+LP+I I  HLPLHTYCFENIS+FK RPCLIN   G IY+Y EV+ T+R+VAAG
Sbjct: 11  DFIFRSRLPDIYIPNHLPLHTYCFENISQFKDRPCLINGPDGRIYSYAEVELTARKVAAG 70

Query: 70  LHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAKVI 129
           L+ LGI +GDVIMLLLQN+PEFV AFLGASY GA  T ANPF+  +EIAKQA +S +K++
Sbjct: 71  LNNLGIQQGDVIMLLLQNSPEFVFAFLGASYRGAVITTANPFYTPAEIAKQATASRSKLL 130

Query: 130 ITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPEDVV 189
           ITQA +AEKVKN ++EN+  IK+I ID   PP+       + + +E E+  VKI+ +DVV
Sbjct: 131 ITQAVYAEKVKNFAKEND--IKIITID--TPPEDCMHFSYLTQSDENEISVVKINSDDVV 190

Query: 190 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLNSI 249
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENP+L+    DV+LC+LPLFHIYSLNS+
Sbjct: 191 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCVLPLFHIYSLNSV 250

Query: 250 MMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSLRI 309
           ++C+LRVGAAIL++QKF++ +L+ELV KYKVT APFVPPIVLAIAK+P VD +D+SS+R+
Sbjct: 251 LLCSLRVGAAILIMQKFEIVTLMELVQKYKVTIAPFVPPIVLAIAKTPDVDKYDLSSIRM 310

Query: 310 VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGTIM 369
           V+SGAAP+GK LEDA RA+LP+  LGQGYGMTEAG V+ M+LAF KE F  KSG CGT++
Sbjct: 311 VMSGAAPMGKELEDAVRARLPNAKLGQGYGMTEAGPVLAMNLAFAKEAFETKSGACGTVV 370

Query: 370 RNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIGFV 429
           RN+EMKI+N +TG SLPRNQ+GEICIR SQ+MKGYLND +AT+  ID+DGWLHTGDIG++
Sbjct: 371 RNAEMKIVNPETGASLPRNQSGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTGDIGYI 430

Query: 430 DDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAFIV 489
           D+DDELFIVDRLKELIKYKGFQVAPAELEA+LI+H +I+DAAV+PM DE AGEVPVAF+V
Sbjct: 431 DEDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEVPVAFVV 490

Query: 490 RFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLAASI 546
           R +GS ITEDEIKQFISKQVVFYKR+ RVFF DSIPK+PSGKILR+ LRA LAA I
Sbjct: 491 RSNGSKITEDEIKQFISKQVVFYKRLARVFFTDSIPKAPSGKILRKDLRARLAAEI 542

BLAST of CsaV3_1G031500 vs. TrEMBL
Match: tr|A0A067KVV8|A0A067KVV8_JATCU (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_08679 PE=4 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 7.0e-215
Identity = 376/533 (70.54%), Postives = 454/533 (85.18%), Query Frame = 0

Query: 10  EFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQATSRRVAAG 69
           +FIFRSKLP+I I  HLPLHTYCFENIS+FK  PCLIN ATG IYTY +V+ TSR+VAAG
Sbjct: 10  DFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNPCLINGATGEIYTYADVELTSRKVAAG 69

Query: 70  LHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQAVSSGAKVI 129
           L KLGI +GDVIMLLLQN+PEFV AFLGAS+ GA +T ANPF+  +EI KQ  +S A+++
Sbjct: 70  LDKLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIKKQLTASKAQLV 129

Query: 130 ITQAAFAEKVKNLSQENEMIIKVIFIDDNDPPQFSSLIEDVAKEEELEMGDVKISPEDVV 189
           ITQA +AEKVK  +++++  IKV+ ID   PP+      ++   +E ++  VKI+ +DVV
Sbjct: 130 ITQAVYAEKVKQFAKDSD--IKVMTID--SPPEDCLHFSELTSCDENDIPTVKINSDDVV 189

Query: 190 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVLCLLPLFHIYSLNSI 249
           ALPYSSGTTG PKGVMLTHKGLVTSVAQQVDGENP+L+    DV+LCLLPLFHIYSLNS+
Sbjct: 190 ALPYSSGTTGFPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSLNSV 249

Query: 250 MMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAKSPAVDHFDMSSLRI 309
           ++C LRVGAAIL++QKF++NSL+ELV+KYKVT APFVPPIVLAIAKSP VD +D+SS+R 
Sbjct: 250 LLCGLRVGAAILIMQKFEINSLMELVTKYKVTIAPFVPPIVLAIAKSPVVDKYDLSSIRT 309

Query: 310 VLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVKEGFGIKSGGCGTIM 369
           V+SGAAP+GK LEDA RAKLP+  LGQGYGMTEAG V++M LAF KE F IKSG CGT++
Sbjct: 310 VMSGAAPMGKELEDAVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACGTVV 369

Query: 370 RNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAIIDEDGWLHTGDIGFV 429
           RN+EMKI++  TG SLPRNQ GEICIR SQ+MKGYLND +AT+  ID+DGWLHTGD+G++
Sbjct: 370 RNAEMKIVDPDTGNSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTGDVGYI 429

Query: 430 DDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPMNDEVAGEVPVAFIV 489
           DDDDELFIVDRLKELIKYKGFQVAPAELEA+LI+H +I+DAAV+PM DE AGE+PVAF+V
Sbjct: 430 DDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVAFVV 489

Query: 490 RFDGSNITEDEIKQFISKQVVFYKRINRVFFVDSIPKSPSGKILRRQLRALLA 543
           R +GS ITEDEIKQ+ISKQV+FYKRI++VFF D+IPK+PSGKILR+ LRA LA
Sbjct: 490 RSNGSKITEDEIKQYISKQVIFYKRISKVFFTDAIPKAPSGKILRKDLRARLA 538