CSPI02G05590 (gene) Wild cucumber (PI 183967)

BLAST of CSPI02G05590 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 3.0e-221
Identity = 372/546 (68.13%), Postives = 459/546 (84.07%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + + +G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDIETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+  K++ITQ  F  KV ++A+E+ +K++C DS+      GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPE----GCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 HNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K +IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of CSPI02G05590 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 3.0e-221
Identity = 371/542 (68.45%), Postives = 455/542 (83.95%), Query Frame = 1

Query: 5   EQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAA 64
           E     D IF+SKLPDIYIP HLPLH+YCFE++S+F  RPCLING   +I+TYAEV L  
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 65  RRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVA 124
           R+ A GL+K+G+ Q D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 125 AKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKI 184
           +  K+IITQ  FV KV ++A E+ VK++C DS+      GCL FSE+ ++DE+EIP VKI
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPE----GCLHFSELTQSDEHEIPEVKI 187

Query: 185 NSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHI 244
             +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+ +DV++CVLPLFHI
Sbjct: 188 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 247

Query: 245 YSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYD 304
           YSLNS+LLCGLRVGAAILI+QK+D++  L LIQ +K +I PFVPPIVL  AK+P +  YD
Sbjct: 248 YSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYD 307

Query: 305 LSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSG 364
           LSS+R VMSGAAP+GK+LED V+ K PNA LGQGYGMTEAGPVL+MCL FAKE F +KSG
Sbjct: 308 LSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 367

Query: 365 ACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHT 424
           ACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TIDK+GWLHT
Sbjct: 368 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHT 427

Query: 425 GDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEI 484
           GD+GFID+DDE+FIVDRLKELIKYKG+QVAPAE+EALL ++PNI+ AAV+PM+DE AGE+
Sbjct: 428 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEV 487

Query: 485 PVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEA 544
           PVAFVVRS GS I+E+++K +IS QVI+YK++++VFFV+++P +PSGKILRK L+A+L A
Sbjct: 488 PVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAA 545

Query: 545 GV 547
           GV
Sbjct: 548 GV 545

BLAST of CSPI02G05590 vs. Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 5.6e-220
Identity = 371/546 (67.95%), Postives = 457/546 (83.70%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MD + + +G D IF+SKLPDIYIP HLPLH+YCFE+LS+F  RPCLI+G   RI+TYAEV
Sbjct: 3   MDTETKQSG-DLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 62

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +R+ A GL+K+G+ Q D IM++L N PEFVFAF+GAS+ GAI+T ANPL+ P E+VK
Sbjct: 63  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 122

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+  K++ITQ  F  KV ++A+E+ +K++C DS       GC+ FSE++++DE+EIP
Sbjct: 123 QAKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPE----GCVHFSELIQSDEHEIP 182

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
            VKI  +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGEN N+ M+  DV++CVLP
Sbjct: 183 DVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLP 242

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLC LRVGAAILI+QK+D++  L LI  HK TI PFVPPIVL  AK+P +
Sbjct: 243 LFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLV 302

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
             YDLSS+R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F 
Sbjct: 303 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 362

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+ EAT +TI+K+G
Sbjct: 363 IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 422

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P+I+ AAV+PM DE 
Sbjct: 423 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 482

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFVVRS GS I+E+++K +IS QVI+YK+I++VFFV+++P +PSGKILRK L+A
Sbjct: 483 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 542

Query: 541 QLEAGV 547
           +L AG+
Sbjct: 543 RLAAGI 543

BLAST of CSPI02G05590 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 3.6e-219
Identity = 372/536 (69.40%), Postives = 456/536 (85.07%), Query Frame = 1

Query: 11  DFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRFAAG 70
           D IF+SKLPDIYIPNHLPLH+YCFE++S+F  RPCLING   +I+TYA+V L +R+ AAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 71  LSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKVKVI 130
           L K G+   D IM++L NSPEFVFAF+GAS+ GAI+T ANPL+ P E+VKQA A+  K+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 131 ITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSNDVV 190
           +TQ   V KV ++A E+ VKI+C DS+      GCL FS + +A+E++IP V+I  +DVV
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPE----GCLHFSVLTQANEHDIPEVEIQPDDVV 188

Query: 191 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLNSV 250
           ALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+ ++ +DV++CVLPLFHIYSLNSV
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSV 248

Query: 251 LLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 310
           LLCGLRVGAAILI+QK+D+ S L LIQ +K TI PFVPPIVL  AK+P +  YDLSS+R 
Sbjct: 249 LLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRT 308

Query: 311 VMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGTVV 370
           VMSGAAP+GK+LEDTV+AK PNA LGQGYGMTEAGPVL+MCL FAKE F++KSGACGTVV
Sbjct: 309 VMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVV 368

Query: 371 RNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLGFI 430
           RNAEMKI+ P+T  SLPRN  GEICIRG QIMKGYLN+ EAT +TIDK+GWL+TGD+G+I
Sbjct: 369 RNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYI 428

Query: 431 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVV 490
           DDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++PNI+ AAV+PM+DE AGE+PVAFVV
Sbjct: 429 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVV 488

Query: 491 RSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAGV 547
           RS GS I+E+++K +IS QVI+YK+I++VFFVD+IP +PSGKILRK L+A+L AG+
Sbjct: 489 RSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGL 540

BLAST of CSPI02G05590 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 9.9e-217
Identity = 369/536 (68.84%), Postives = 453/536 (84.51%), Query Frame = 1

Query: 10  KDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRFAA 69
           KD IF+SKLPDIYIP +LPLH+YCFE++S+F  RPCLING T  I TYA+V L +RR  +
Sbjct: 12  KDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVGS 71

Query: 70  GLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKVKV 129
           GLSK+G+ QGD IM++L NSPEFVFAFLGASF G+I+T ANP +   E++KQA A+  K+
Sbjct: 72  GLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNAKL 131

Query: 130 IITQGEFVEKVWEFAVEHGVKILCTDSSS-ASESAGCLKFSEVMEADENEIPAVKINSND 189
           IITQG +V+KV ++A E+GVKI+  D+++ A ++A  L FSE+  ADENE+P V+I+ + 
Sbjct: 132 IITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISPDG 191

Query: 190 VVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLN 249
           VVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+ M+  DV++CVLPLFHIYSLN
Sbjct: 192 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYSLN 251

Query: 250 SVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSI 309
           SVLLCGLR G+ ILI+QK+++   L LIQ +K TI PFVPPIVL  AK+  +  YDLSS+
Sbjct: 252 SVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLSSV 311

Query: 310 RIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGT 369
           R VMSGAAP+GK+LED V+AK PNA LGQGYGMTEAGPVL+MCL FAKE F +KSGACGT
Sbjct: 312 RTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 371

Query: 370 VVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLG 429
           VVRNAEMKI+ P+T  SLPRN+PGEICIRG QIMKGYLN+ EAT +TIDK+GWLHTGD+G
Sbjct: 372 VVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGDIG 431

Query: 430 FIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAF 489
           +IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL ++P I+ AAV+PM+DEAAGE+PVAF
Sbjct: 432 YIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPVAF 491

Query: 490 VVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEA 545
           VV+S G  I+E++IK +IS QVI+YK+I +VFFV++IP APSGKILRK L+A+L A
Sbjct: 492 VVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAA 547

BLAST of CSPI02G05590 vs. TrEMBL
Match: A0A0A0LM95_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G048330 PE=4 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 543/547 (99.27%), Postives = 546/547 (99.82%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQ NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIK+YISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of CSPI02G05590 vs. TrEMBL
Match: J7FW91_COFAR (4-hydroxycinnamoyl-CoA ligase 3 OS=Coffea arabica GN=4CL3 PE=2 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.1e-230
Identity = 394/543 (72.56%), Postives = 474/543 (87.29%), Query Frame = 1

Query: 4   DEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLA 63
           + + + ++F+F+SKLPDIYIPNHLPLH+YCFE++ +F +RPCLINGTTG I+TYA+V L 
Sbjct: 2   ETKQDQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELI 61

Query: 64  ARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAV 123
           AR+ AAGL KIG+ QG+VIML+LQNSPEF FA LGAS+ GA++TTANP YKP EI KQA 
Sbjct: 62  ARKVAAGLDKIGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQAR 121

Query: 124 AAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVK 183
           A+K K+IITQ  +VEKV +FA E+ VK++CTD+       GCL FSE+  ADE  IPAVK
Sbjct: 122 ASKAKLIITQSCYVEKVMDFAKENNVKVMCTDAPPE----GCLHFSELSSADEKVIPAVK 181

Query: 184 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFH 243
           IN ND VALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +++DVI+CVLPLFH
Sbjct: 182 INPNDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 241

Query: 244 IYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 303
           IYSLNSVLLCGLRVGAAILI+QK+++++L+ L+Q +K TIAPFVPPIVL+ AK+P + +Y
Sbjct: 242 IYSLNSVLLCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKY 301

Query: 304 DLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKS 363
           DLSSIR+VMSGAAPMGK+LEDTV+AKLP A+LGQGYGMTEAGP+LSMCL FAKE F VKS
Sbjct: 302 DLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKS 361

Query: 364 GACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLH 423
           GACGTVVRNAEMKI+ P+T+LSLPRN  GEICIRG QIMKGYLN+ EATE TIDK+GWLH
Sbjct: 362 GACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLH 421

Query: 424 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 483
           TGD+G+IDDDDEIFIVDRLKELIKYKG+QVAPAELEA+L S+P I+ AAV+ M+DEAAGE
Sbjct: 422 TGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGE 481

Query: 484 IPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLE 543
           +PVAFVVR+ GSKISE++IK +IS+QVI+YK+I +VFF+D IP APSGKILRK L+A+L 
Sbjct: 482 VPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAKLA 540

Query: 544 AGV 547
           A V
Sbjct: 542 AEV 540

BLAST of CSPI02G05590 vs. TrEMBL
Match: Q9LL50_RUBID (4-coumarate:coA ligase 2 OS=Rubus idaeus PE=2 SV=1)

HSP 1 Score: 805.4 bits (2079), Expect = 4.2e-230
Identity = 393/543 (72.38%), Postives = 465/543 (85.64%), Query Frame = 1

Query: 4   DEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLA 63
           ++  +  +FIF+SKLPDIYIPNHLPLHTYCFE++SQF  RPCLING TG   TYAEV L 
Sbjct: 3   NKHQDDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELT 62

Query: 64  ARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAV 123
           +RR AAGL K+G+ Q DV+ML+LQN PEF FAFLGAS+ GA++TTANP Y P E+ KQA 
Sbjct: 63  SRRVAAGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAK 122

Query: 124 AAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVK 183
           A+  K+IITQ  +V+KV +FA  + VK++C D +S+ +    L FSE+M ADE+E PAVK
Sbjct: 123 ASNAKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSED---VLHFSELMSADESETPAVK 182

Query: 184 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFH 243
           IN +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +++DVI+CVLPLFH
Sbjct: 183 INPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 242

Query: 244 IYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 303
           IYSLNSV LCGLRVGAAILI+QK++++ LL L++  K TIAPFVPPIVL  AK PD+HRY
Sbjct: 243 IYSLNSVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRY 302

Query: 304 DLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKS 363
           DLSSIR+VMSGAAPMGK+LEDTV+AKLPNA LGQGYGMTEAGPVLSMCL FAKE +++KS
Sbjct: 303 DLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKS 362

Query: 364 GACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLH 423
           GACGTVVRNAEMKII P T+ SLPRN  GEICIRG QIMKGYLN+ EATE TIDK+GWLH
Sbjct: 363 GACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLH 422

Query: 424 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 483
           TGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEA+L S+PN++ AAV+ M+DEAAGE
Sbjct: 423 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGE 482

Query: 484 IPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLE 543
           +PVAFVVRS GSKISE+DIK YIS QV++YK+I KVFF D IP APSGKILRK L+A+L 
Sbjct: 483 VPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLA 542

Query: 544 AGV 547
           AG+
Sbjct: 543 AGL 542

BLAST of CSPI02G05590 vs. TrEMBL
Match: K4JWH2_PUNGR (4-coumarate:coenzyme A ligase OS=Punica granatum GN=4CL PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 9.3e-230
Identity = 388/546 (71.06%), Postives = 474/546 (86.81%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+  +    ++FIF+SKLPDIYIPNHLPLH+YCFE++S+FQ RPCLI+G+TGRI+TY+EV
Sbjct: 1   METKQDTVSQEFIFRSKLPDIYIPNHLPLHSYCFENISEFQDRPCLIDGSTGRIYTYSEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
            L +RR A+GL+K+G+ QG VIML+L NSPEFV AFLGASF GA++TTANP   P EI K
Sbjct: 61  ELTSRRVASGLNKLGIEQGQVIMLLLHNSPEFVHAFLGASFCGAMSTTANPFCTPPEIAK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QA A+K ++IITQ EF  KV +FA E+GVK++C DS+      GCL FSE+ +ADE E+P
Sbjct: 121 QAAASKARLIITQAEFAPKVKDFAEENGVKVMCIDSAPE----GCLHFSELTQADEAEMP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           +VKI+ NDVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+   ++DVI+CVLP
Sbjct: 181 SVKIDPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRKEDVILCVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNS++LCGLRVGAAILI+QK+++  L+ L+Q +K TIAPFVPPIVL  +K  D+
Sbjct: 241 LFHIYSLNSIMLCGLRVGAAILIMQKFEIVKLMELVQRYKVTIAPFVPPIVLAMSKYADM 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
            +YDLSSIR VMSGAAPMGK+LED+V+AKLPNA LGQGYGMTEAGPVL+MCL FAKE F+
Sbjct: 301 DKYDLSSIRTVMSGAAPMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEPFE 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           +KSGACGTVVRNAEMKI++P T  SLPRN PGEICIRG QIMKGYLN+ EATE+TIDKDG
Sbjct: 361 IKSGACGTVVRNAEMKIVNPDTGASLPRNQPGEICIRGNQIMKGYLNDPEATERTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEA+L ++P+I+ AAV+PM+DEA
Sbjct: 421 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISDAAVVPMKDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGE+PVAFV+RS GSKI+E+DIK YIS QV++YK+I +VFF ++IP APSGKILRK L+A
Sbjct: 481 AGEVPVAFVIRSNGSKITEDDIKQYISKQVVFYKRINRVFFTEAIPKAPSGKILRKYLRA 540

Query: 541 QLEAGV 547
           +L +GV
Sbjct: 541 KLASGV 542

BLAST of CSPI02G05590 vs. TrEMBL
Match: A0A067KVV8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_08679 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 3.5e-229
Identity = 394/538 (73.23%), Postives = 461/538 (85.69%), Query Frame = 1

Query: 8   NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRF 67
           N +DFIF+SKLPDIYIPNHLPLHTYCFE++SQF+  PCLING TG I+TYA+V L +R+ 
Sbjct: 7   NQEDFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNPCLINGATGEIYTYADVELTSRKV 66

Query: 68  AAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKV 127
           AAGL K+G+ QGDVIML+LQNSPEFVFAFLGASF GAI+TTANP Y P EI KQ  A+K 
Sbjct: 67  AAGLDKLGIQQGDVIMLLLQNSPEFVFAFLGASFRGAISTTANPFYTPAEIKKQLTASKA 126

Query: 128 KVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVKINSN 187
           +++ITQ  + EKV +FA +  +K++  DS        CL FSE+   DEN+IP VKINS+
Sbjct: 127 QLVITQAVYAEKVKQFAKDSDIKVMTIDSPPED----CLHFSELTSCDENDIPTVKINSD 186

Query: 188 DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSL 247
           DVVALP+SSGTTG PKGVMLTHK LVTSVAQQVDGENPN+  + KDVI+C+LPLFHIYSL
Sbjct: 187 DVVALPYSSGTTGFPKGVMLTHKGLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHIYSL 246

Query: 248 NSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSS 307
           NSVLLCGLRVGAAILI+QK++++SL+ L+  +K TIAPFVPPIVL  AK+P + +YDLSS
Sbjct: 247 NSVLLCGLRVGAAILIMQKFEINSLMELVTKYKVTIAPFVPPIVLAIAKSPVVDKYDLSS 306

Query: 308 IRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACG 367
           IR VMSGAAPMGK+LED V+AKLPNA LGQGYGMTEAGPVLSMCL FAKE F++KSGACG
Sbjct: 307 IRTVMSGAAPMGKELEDAVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEIKSGACG 366

Query: 368 TVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDL 427
           TVVRNAEMKI+ P T  SLPRN  GEICIRG QIMKGYLN+ EATE+TIDKDGWLHTGD+
Sbjct: 367 TVVRNAEMKIVDPDTGNSLPRNQAGEICIRGSQIMKGYLNDPEATERTIDKDGWLHTGDV 426

Query: 428 GFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVA 487
           G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEA+L ++PNI+ AAV+PM+DEAAGEIPVA
Sbjct: 427 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPNISDAAVVPMKDEAAGEIPVA 486

Query: 488 FVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLEAG 546
           FVVRS GSKI+E++IK YIS QVI+YK+I KVFF D+IP APSGKILRK L+A+L  G
Sbjct: 487 FVVRSNGSKITEDEIKQYISKQVIFYKRISKVFFTDAIPKAPSGKILRKDLRARLADG 540

BLAST of CSPI02G05590 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 713.8 bits (1841), Expect = 8.3e-206
Identity = 352/550 (64.00%), Postives = 437/550 (79.45%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           M+     N  D IF+SKLPDIYIPNHL LH Y F+++S+F  +PCLING TG ++TY++V
Sbjct: 12  MEKQSNNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDV 71

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           ++ +R+ AA   K+GV Q DV+ML+L N PEFV +FL ASF GA AT ANP + P EI K
Sbjct: 72  HVISRQIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAK 131

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSS-SASESAGCLKFSEVMEADENE- 180
           QA A+  K+IIT+  +V+K+     + GV I+C D + S     GCL+F+E+ ++     
Sbjct: 132 QAKASNTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEAS 191

Query: 181 --IPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVII 240
             I +V+I+ +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +  DVI+
Sbjct: 192 EVIDSVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVIL 251

Query: 241 CVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAK 300
           CVLP+FHIY+LNS++LCGLRVGAAILI+ K++++ LL LIQ  K T+AP VPPIVL  AK
Sbjct: 252 CVLPMFHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAK 311

Query: 301 NPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAK 360
           + +  +YDLSSIR+V SGAAP+GK+LED V AK PNA LGQGYGMTEAGPVL+M LGFAK
Sbjct: 312 SSETEKYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 371

Query: 361 EAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTI 420
           E F VKSGACGTVVRNAEMKI+ P T  SL RN PGEICIRG QIMKGYLNN  AT +TI
Sbjct: 372 EPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETI 431

Query: 421 DKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPM 480
           DKDGWLHTGD+G IDDDDE+FIVDRLKELIKYKG+QVAPAELEALL  +P+IT  AV+ M
Sbjct: 432 DKDGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAM 491

Query: 481 EDEAAGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRK 540
           ++EAAGE+PVAFVV+SK S++SE+D+K ++S QV++YK+I KVFF +SIP APSGKILRK
Sbjct: 492 KEEAAGEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551

Query: 541 ILKAQLEAGV 547
            L+A+L  G+
Sbjct: 552 DLRAKLANGL 561

BLAST of CSPI02G05590 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 711.8 bits (1836), Expect = 3.2e-205
Identity = 353/546 (64.65%), Postives = 437/546 (80.04%), Query Frame = 1

Query: 3   HDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNL 62
           +D++    D IF+S+LPDIYIPNHLPLH Y FE++S+F  +PCLING TG ++TYA+V++
Sbjct: 12  NDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHV 71

Query: 63  AARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQA 122
            +R+ AAGL  +GV Q DV+M++L NSPE V  FL ASF GAI T+ANP + P EI KQA
Sbjct: 72  TSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQA 131

Query: 123 VAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAV 182
            A+  K+I+TQ  +V+K+     + GV I+ TDS +  E+  CL+FSE+ +++E  + ++
Sbjct: 132 KASAAKLIVTQSRYVDKIKNLQND-GVLIVTTDSDAIPEN--CLRFSELTQSEEPRVDSI 191

Query: 183 --KINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 242
             KI+  DVVALPFSSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  NR DVI+CVLP
Sbjct: 192 PEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLP 251

Query: 243 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 302
           +FHIY+LNS++LC LRVGA ILI+ K++++ LL  IQ  K T+A  VPPIVL  AK+P+ 
Sbjct: 252 MFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPET 311

Query: 303 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 362
            +YDLSS+R+V SGAAP+GK+LED + AK PNA LGQGYGMTEAGPVL+M LGFAKE F 
Sbjct: 312 EKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFP 371

Query: 363 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 422
           VKSGACGTVVRNAEMKI+ P T  SLPRN PGEICIRG QIMKGYLN+  AT  TIDKDG
Sbjct: 372 VKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDG 431

Query: 423 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 482
           WLHTGD+GFIDDDDE+FIVDRLKELIKYKG+QVAPAELE+LL  +P I   AV+ M++E 
Sbjct: 432 WLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEED 491

Query: 483 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 542
           AGE+PVAFVVRSK S ISE++IK ++S QV++YK+I KVFF DSIP APSGKILRK L+A
Sbjct: 492 AGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRA 551

Query: 543 QLEAGV 547
           +L  G+
Sbjct: 552 RLANGL 554

BLAST of CSPI02G05590 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 661.4 bits (1705), Expect = 4.9e-190
Identity = 324/532 (60.90%), Postives = 415/532 (78.01%), Query Frame = 1

Query: 13  IFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARRFAAGLS 72
           IF+SKLPDI IPNHLPLHTYCFE LS    +PCLI G+TG+ +TY E +L  RR A+GL 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 73  KIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAKVKVIIT 132
           K+G+ +GDVIM++LQNS EFVF+F+GAS  GA++TTANP Y   E+ KQ  ++  K+IIT
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 133 QGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEI--PAVKINSNDVV 192
             ++V+K+        + ++ TD  +      CL FS ++  DE       V I  +D  
Sbjct: 154 HSQYVDKLKNLG--ENLTLITTDEPTPEN---CLPFSTLITDDETNPFQETVDIGGDDAA 213

Query: 193 ALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFHIYSLNSV 252
           ALPFSSGTTG+PKGV+LTHKSL+TSVAQQVDG+NPN+ +   DVI+CVLPLFHIYSLNSV
Sbjct: 214 ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSV 273

Query: 253 LLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRYDLSSIRI 312
           LL  LR GA +L++ K+++ +LL LIQ H+ TIA  VPP+V+  AKNP ++ YDLSS+R 
Sbjct: 274 LLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF 333

Query: 313 VMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKSGACGTVV 372
           V+SGAAP+GK+L+D+++ +LP AILGQGYGMTEAGPVLSM LGFAKE    KSG+CGTVV
Sbjct: 334 VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVV 393

Query: 373 RNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLHTGDLGFI 432
           RNAE+K++H +T LSL  N PGEICIRG QIMK YLN+ EAT  TID++GWLHTGD+G++
Sbjct: 394 RNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYV 453

Query: 433 DDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGEIPVAFVV 492
           D+DDEIFIVDRLKE+IK+KG+QV PAELE+LL ++ +I  AAV+P  DE AGE+PVAFVV
Sbjct: 454 DEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVV 513

Query: 493 RSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQL 543
           RS G+ I+E D+K Y++ QV++YK++ KVFFV SIP +PSGKILRK LKA+L
Sbjct: 514 RSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CSPI02G05590 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-185
Identity = 316/551 (57.35%), Postives = 420/551 (76.23%), Query Frame = 1

Query: 2   DHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQF----QHRPCLINGTTGRIHTY 61
           + +E+    DFIF+SKLPDI+IPNHLPL  Y F+  S          C+I+G TGRI TY
Sbjct: 17  EDEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTY 76

Query: 62  AEVNLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGE 121
           A+V    RR AAG+ ++G+  GDV+ML+L NSPEF  +FL  ++ GA++TTANP Y   E
Sbjct: 77  ADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPE 136

Query: 122 IVKQAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSS-----ASESAGCLKFSEVM 181
           I KQA A+  K+IIT+   V+K+     + GV I+C D        +S   GC+ F+E+ 
Sbjct: 137 IAKQAKASAAKMIITKKCLVDKLTNLKND-GVLIVCLDDDGDNGVVSSSDDGCVSFTELT 196

Query: 182 EADENEIPAVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRK 241
           +ADE E+   KI+  D VA+P+SSGTTG+PKGVM+THK LVTS+AQ+VDGENPN+N    
Sbjct: 197 QADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAN 256

Query: 242 DVIICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVL 301
           DVI+C LP+FHIY+L++++L  +R GAA+LI+ +++++ ++ LIQ +K T+ P  PP+VL
Sbjct: 257 DVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVL 316

Query: 302 DFAKNPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCL 361
            F K+P+  RYDLSS+RI++SGAA + K+LED V+ K PNAI GQGYGMTE+G V +  L
Sbjct: 317 AFIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSL 376

Query: 362 GFAKEAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEAT 421
            FAK  FK KSGACGTV+RNAEMK++  +T +SLPRN  GEIC+RG Q+MKGYLN+ EAT
Sbjct: 377 AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEAT 436

Query: 422 EKTIDKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAA 481
            +TIDKDGWLHTGD+GF+DDDDEIFIVDRLKELIK+KGYQVAPAELEALL S+P+I  AA
Sbjct: 437 ARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAA 496

Query: 482 VIPMEDEAAGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGK 541
           V+ M+DE A E+PVAFV RS+GS+++E+D+KSY++ QV++YK+I+ VFF++ IP A SGK
Sbjct: 497 VVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGK 556

Query: 542 ILRKILKAQLE 544
           ILRK L+A+LE
Sbjct: 557 ILRKDLRAKLE 565

BLAST of CSPI02G05590 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 384.8 bits (987), Expect = 8.9e-107
Identity = 212/550 (38.55%), Postives = 328/550 (59.64%), Query Frame = 1

Query: 9   GKDFIFKSKLPDIYIPN--HLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLAARR 68
           G+D I++S  P + +P   +  L ++ F + S +  +  + +  TG   T++++  A  R
Sbjct: 7   GRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVAR 66

Query: 69  FAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAVAAK 128
            A G  ++G+ + DV+++   NS +F   FL  +  G + TTANPLY   E+ KQ   + 
Sbjct: 67  LAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSN 126

Query: 129 VKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASE-----SAGCLKFSEVMEADE--NEI 188
            K+II+  +  +K+  F     + ++   S    E     ++  L F  VME  E  +E 
Sbjct: 127 PKIIISVNQLFDKIKGF----DLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVSEY 186

Query: 189 PAVKINSNDVVALPFSSGTTGVPKGVMLTH-----KSLVTSVAQQVDGENPNVNMNRKDV 248
           P V+I  +D  AL +SSGTTG  KGV LTH      SL+ ++ Q + GE          V
Sbjct: 187 PFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGE-------YHGV 246

Query: 249 IICVLPLFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDF 308
            +C LP+FH++ L  +    L+ G A++ + ++++  +L+ I+  + T    VPP+ L  
Sbjct: 247 FLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLAL 306

Query: 309 AKNPDIHRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGF 368
           +K   + ++DLSS++ + SGAAP+GKDL +     +PN +L QGYGMTE   ++S+    
Sbjct: 307 SKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSV---E 366

Query: 369 AKEAFKVKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEK 428
                K  SG+ G +    E +I+  +T  S P N  GEI +RGP +MKGYLNN +AT++
Sbjct: 367 DPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKE 426

Query: 429 TIDKDGWLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVI 488
           TIDK  W+HTGDLG+ ++D  +++VDR+KELIKYKG+QVAPAELE LL S+P+I  A VI
Sbjct: 427 TIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVI 486

Query: 489 PMEDEAAGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKIL 545
           P  DE AGE+P+AFVVRS  S I+E DI+ +I+ QV  YK++R+V F+  +P + +GKIL
Sbjct: 487 PFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKIL 542

BLAST of CSPI02G05590 vs. NCBI nr
Match: gi|449462693|ref|XP_004149075.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis sativus])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 543/547 (99.27%), Postives = 546/547 (99.82%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDHDEQ NGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDHDEQRNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP
Sbjct: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINS+DVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP
Sbjct: 181 AVKINSSDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK
Sbjct: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLG+IDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGYIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIK+YISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 547

BLAST of CSPI02G05590 vs. NCBI nr
Match: gi|659069891|ref|XP_008452293.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 1038.5 bits (2684), Expect = 4.2e-300
Identity = 522/547 (95.43%), Postives = 539/547 (98.54%), Query Frame = 1

Query: 1   MDHDEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEV 60
           MDH EQ NG+DFIFKSKLPDIYIPNHLPLHTYCFEHL+QFQHRPCLINGTTGRIHTYAEV
Sbjct: 1   MDH-EQRNGQDFIFKSKLPDIYIPNHLPLHTYCFEHLAQFQHRPCLINGTTGRIHTYAEV 60

Query: 61  NLAARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120
           +LAARRFAAGLSKIG+GQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK
Sbjct: 61  DLAARRFAAGLSKIGIGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVK 120

Query: 121 QAVAAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIP 180
           QAVAA+ KVIITQGEFVEKVWEFAVE+GVKILCTDSSSASESAGCL+FSEVMEADEN+IP
Sbjct: 121 QAVAARAKVIITQGEFVEKVWEFAVENGVKILCTDSSSASESAGCLRFSEVMEADENDIP 180

Query: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLP 240
           AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMN KDVIICVLP
Sbjct: 181 AVKINSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNSKDVIICVLP 240

Query: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDI 300
           LFHIYSLNSVLLCGLRVGAAILILQKYD+SSLLRLIQ HKATIAPFVPPIVLDFAKNPDI
Sbjct: 241 LFHIYSLNSVLLCGLRVGAAILILQKYDMSSLLRLIQIHKATIAPFVPPIVLDFAKNPDI 300

Query: 301 HRYDLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFK 360
           H Y+LSSIRIVMSGAAPMGKDLEDTVKA+LPNAILGQGYGMTEAGPVLSMCLGFAKE F 
Sbjct: 301 HNYNLSSIRIVMSGAAPMGKDLEDTVKARLPNAILGQGYGMTEAGPVLSMCLGFAKEPFN 360

Query: 361 VKSGACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDG 420
           VKSGACGTVVRNAEMKIIHPQ+SLSLPRN+PGEICIRGPQIMKGYLNN+EATEKTIDKDG
Sbjct: 361 VKSGACGTVVRNAEMKIIHPQSSLSLPRNHPGEICIRGPQIMKGYLNNEEATEKTIDKDG 420

Query: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480
           WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA
Sbjct: 421 WLHTGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEA 480

Query: 481 AGEIPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKA 540
           AGEIPVAFVVRSKGSKISENDIK+YISDQVIYYK+IRKVFFVDSIPMAPSGKILRK+LKA
Sbjct: 481 AGEIPVAFVVRSKGSKISENDIKNYISDQVIYYKRIRKVFFVDSIPMAPSGKILRKVLKA 540

Query: 541 QLEAGVF 548
           QLEAGVF
Sbjct: 541 QLEAGVF 546

BLAST of CSPI02G05590 vs. NCBI nr
Match: gi|399630459|gb|AFP49810.1| (4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica])

HSP 1 Score: 807.4 bits (2084), Expect = 1.6e-230
Identity = 394/543 (72.56%), Postives = 474/543 (87.29%), Query Frame = 1

Query: 4   DEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLA 63
           + + + ++F+F+SKLPDIYIPNHLPLH+YCFE++ +F +RPCLINGTTG I+TYA+V L 
Sbjct: 2   ETKQDQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVELI 61

Query: 64  ARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAV 123
           AR+ AAGL KIG+ QG+VIML+LQNSPEF FA LGAS+ GA++TTANP YKP EI KQA 
Sbjct: 62  ARKVAAGLDKIGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQAR 121

Query: 124 AAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVK 183
           A+K K+IITQ  +VEKV +FA E+ VK++CTD+       GCL FSE+  ADE  IPAVK
Sbjct: 122 ASKAKLIITQSCYVEKVMDFAKENNVKVMCTDAPPE----GCLHFSELSSADEKVIPAVK 181

Query: 184 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFH 243
           IN ND VALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +++DVI+CVLPLFH
Sbjct: 182 INPNDAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 241

Query: 244 IYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 303
           IYSLNSVLLCGLRVGAAILI+QK+++++L+ L+Q +K TIAPFVPPIVL+ AK+P + +Y
Sbjct: 242 IYSLNSVLLCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDKY 301

Query: 304 DLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKS 363
           DLSSIR+VMSGAAPMGK+LEDTV+AKLP A+LGQGYGMTEAGP+LSMCL FAKE F VKS
Sbjct: 302 DLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPFDVKS 361

Query: 364 GACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLH 423
           GACGTVVRNAEMKI+ P+T+LSLPRN  GEICIRG QIMKGYLN+ EATE TIDK+GWLH
Sbjct: 362 GACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGWLH 421

Query: 424 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 483
           TGD+G+IDDDDEIFIVDRLKELIKYKG+QVAPAELEA+L S+P I+ AAV+ M+DEAAGE
Sbjct: 422 TGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAAGE 481

Query: 484 IPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLE 543
           +PVAFVVR+ GSKISE++IK +IS+QVI+YK+I +VFF+D IP APSGKILRK L+A+L 
Sbjct: 482 VPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAKLA 540

Query: 544 AGV 547
           A V
Sbjct: 542 AEV 540

BLAST of CSPI02G05590 vs. NCBI nr
Match: gi|9651915|gb|AAF91309.1|AF239686_1 (4-coumarate:coA ligase 2 [Rubus idaeus])

HSP 1 Score: 805.4 bits (2079), Expect = 6.0e-230
Identity = 393/543 (72.38%), Postives = 465/543 (85.64%), Query Frame = 1

Query: 4   DEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLA 63
           ++  +  +FIF+SKLPDIYIPNHLPLHTYCFE++SQF  RPCLING TG   TYAEV L 
Sbjct: 3   NKHQDDHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGETFTYAEVELT 62

Query: 64  ARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAV 123
           +RR AAGL K+G+ Q DV+ML+LQN PEF FAFLGAS+ GA++TTANP Y P E+ KQA 
Sbjct: 63  SRRVAAGLDKLGIQQNDVVMLLLQNCPEFAFAFLGASYIGAMSTTANPFYTPAEVAKQAK 122

Query: 124 AAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVK 183
           A+  K+IITQ  +V+KV +FA  + VK++C D +S+ +    L FSE+M ADE+E PAVK
Sbjct: 123 ASNAKLIITQSAYVDKVKDFAKLNDVKVMCVDETSSED---VLHFSELMSADESETPAVK 182

Query: 184 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFH 243
           IN +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +++DVI+CVLPLFH
Sbjct: 183 INPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 242

Query: 244 IYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 303
           IYSLNSV LCGLRVGAAILI+QK++++ LL L++  K TIAPFVPPIVL  AK PD+HRY
Sbjct: 243 IYSLNSVFLCGLRVGAAILIMQKFEINKLLELVEKEKVTIAPFVPPIVLSIAKCPDLHRY 302

Query: 304 DLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKS 363
           DLSSIR+VMSGAAPMGK+LEDTV+AKLPNA LGQGYGMTEAGPVLSMCL FAKE +++KS
Sbjct: 303 DLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYEIKS 362

Query: 364 GACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLH 423
           GACGTVVRNAEMKII P T+ SLPRN  GEICIRG QIMKGYLN+ EATE TIDK+GWLH
Sbjct: 363 GACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATENTIDKEGWLH 422

Query: 424 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 483
           TGD+G+IDDDDE+FIVDRLKELIKYKG+QVAPAELEA+L S+PN++ AAV+ M+DEAAGE
Sbjct: 423 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVSMKDEAAGE 482

Query: 484 IPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLE 543
           +PVAFVVRS GSKISE+DIK YIS QV++YK+I KVFF D IP APSGKILRK L+A+L 
Sbjct: 483 VPVAFVVRSNGSKISEDDIKQYISKQVVFYKRISKVFFTDKIPKAPSGKILRKDLRARLA 542

Query: 544 AGV 547
           AG+
Sbjct: 543 AGL 542

BLAST of CSPI02G05590 vs. NCBI nr
Match: gi|470148826|ref|XP_004309949.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 805.4 bits (2079), Expect = 6.0e-230
Identity = 390/543 (71.82%), Postives = 468/543 (86.19%), Query Frame = 1

Query: 4   DEQMNGKDFIFKSKLPDIYIPNHLPLHTYCFEHLSQFQHRPCLINGTTGRIHTYAEVNLA 63
           + + +  +FIF+SKLPDIYIPNHLPLHTYCFE++SQF  RPCLING TG+  TYA+V L 
Sbjct: 2   ENKQDNHEFIFRSKLPDIYIPNHLPLHTYCFENISQFHDRPCLINGNTGQTFTYADVELT 61

Query: 64  ARRFAAGLSKIGVGQGDVIMLMLQNSPEFVFAFLGASFAGAIATTANPLYKPGEIVKQAV 123
           +RR AAGL K+G+ QGDV+ML+LQN PEF FAFLGAS+ GAI TTANP Y P E+ KQA 
Sbjct: 62  SRRVAAGLDKLGIQQGDVVMLLLQNCPEFAFAFLGASYIGAIITTANPFYTPAEVAKQAT 121

Query: 124 AAKVKVIITQGEFVEKVWEFAVEHGVKILCTDSSSASESAGCLKFSEVMEADENEIPAVK 183
           A+K K+IITQ  +VEKV +FA  + VK++  D +S+ +    L FSE+  ADE++IPAVK
Sbjct: 122 ASKAKLIITQTAYVEKVKDFAKLNNVKVMSVDEASSCDK-DVLHFSELTSADESDIPAVK 181

Query: 184 INSNDVVALPFSSGTTGVPKGVMLTHKSLVTSVAQQVDGENPNVNMNRKDVIICVLPLFH 243
           IN +DVVALP+SSGTTG+PKGVMLTHK LVTSVAQQVDGENPN+  +++DVI+CVLPLFH
Sbjct: 182 INPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLFH 241

Query: 244 IYSLNSVLLCGLRVGAAILILQKYDVSSLLRLIQTHKATIAPFVPPIVLDFAKNPDIHRY 303
           IYSLNSV LCGLRVGA ILI+QK++++ LL L++  K TIAPFVPPIVL+ AK+PD+H+Y
Sbjct: 242 IYSLNSVFLCGLRVGATILIMQKFEINKLLELVEKEKVTIAPFVPPIVLNIAKSPDLHQY 301

Query: 304 DLSSIRIVMSGAAPMGKDLEDTVKAKLPNAILGQGYGMTEAGPVLSMCLGFAKEAFKVKS 363
           DLSSIR+VMSGAAPMGK+LEDTV+AKLPNA LGQGYGMTEAGPVLSMCL FAKE + +KS
Sbjct: 302 DLSSIRMVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPYPIKS 361

Query: 364 GACGTVVRNAEMKIIHPQTSLSLPRNNPGEICIRGPQIMKGYLNNKEATEKTIDKDGWLH 423
           GACGTVVRNAEMKII P T+ SLPRN  GEICIRG QIMKGYLN+ EATE+TIDK+GWLH
Sbjct: 362 GACGTVVRNAEMKIIDPDTNESLPRNQSGEICIRGSQIMKGYLNDPEATERTIDKEGWLH 421

Query: 424 TGDLGFIDDDDEIFIVDRLKELIKYKGYQVAPAELEALLTSNPNITSAAVIPMEDEAAGE 483
           TGD+G+IDDD+E+FIVDRLKELIKYKG+QVAPAELEA+L S+PN++ AAV+PM+DEAAGE
Sbjct: 422 TGDIGYIDDDEELFIVDRLKELIKYKGFQVAPAELEAMLISHPNLSDAAVVPMKDEAAGE 481

Query: 484 IPVAFVVRSKGSKISENDIKSYISDQVIYYKKIRKVFFVDSIPMAPSGKILRKILKAQLE 543
           +P+AFVVRS GSKISE+DIK Y+S QV++YK+I +VFF D IP APSGKILRK L+A+L 
Sbjct: 482 VPIAFVVRSNGSKISEDDIKQYVSKQVVFYKRISRVFFTDKIPKAPSGKILRKDLRARLA 541

Query: 544 AGV 547
           AGV
Sbjct: 542 AGV 543