Carg04251 (gene) Silver-seed gourd

NameCarg04251
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description2-oxoglutarate-dependent dioxygenase
LocationCucurbita_argyrosperma_scaffold_044 : 1056541 .. 1058177 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATGTGGCCTTCTGGAAATCATCATTTCTGGTAAACTTTGTATGAATTACATGGTTGTCTATTTTGGGTGTAAATCTTTAAAATCTTACTTGGAAATGATTTTGTTGGACAGTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGTCAATGGCTTGGAAATCAAAGCAAAAAAGGATGATCGTTGGATTAGACTTCAATTCAATCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTATACCTCTTACTTGCACTTTCATCCATACTTCAATTATACACACACATATACGGTAATAGTCCAAGTCCACCGCTAACAGATATTGTTCTGTTTGGACTTCCCCTCAAGATTTTTAAAATGCTTATGTTAGACAGAGGTTTCCACACTCGATGCGGGATCTCACAATCCACTCCCCTTCGGGGCCAACGTTCTTGCTGACACTCGTTCCCTTCTCTAATCGATGTGGGACCCCCAATCCACCCTCTTTGGGGTCCAACGTCCTTCCTAACACATCACCCGATGCTTAGCTTTGATATCATTTGTAACAGCCCAAACTCACTACTAGTAGATATTGTTTTCTTTAGGCTTTCCCTAACTCACTACTAGTAGATATTGCCTTCTTTAGGCTTTCCCTTTTGGTCTTCTCCTCAAAGTTTTTAAAGACTCGTCTACTGGGGAGAGATTTCCACACCCTTATAAAGAATGATTCGTTTTCTTCCTAACGTGAGATCTCACATGTAGTTATCAATTGTTTATCCCAAAGATTGATGATTTGAATACTTGAATTAAGTATTGAATGGTAAGAACAAAATTTGATTTTGATGGATAGGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Protein sequence

MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV
BLAST of Carg04251 vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 546.2 bits (1406), Expect = 6.4e-152
Identity = 275/320 (85.94%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENL PKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         QDLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

BLAST of Carg04251 vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538.1 bits (1385), Expect = 1.8e-149
Identity = 269/320 (84.06%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSST PEVPVI+FSIENLRPKSTKWVETC+HVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIP+ELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHYGLLG 300

BLAST of Carg04251 vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 522.3 bits (1344), Expect = 1.0e-144
Identity = 264/320 (82.50%), Postives = 270/320 (84.38%), Query Frame = 0

Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSS KPEVP INFSIENLRPKSTKWVETC+HVRAALEEYGCFLAE EN+    VELD
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECENN----VELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRV+IEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

BLAST of Carg04251 vs. NCBI nr
Match: XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 1.4e-114
Identity = 212/315 (67.30%), Postives = 240/315 (76.19%), Query Frame = 0

Query: 5   SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
           S  K ++PVIN SIEN+ P+ST W+E C+H+RAALEEYGCFL E+ +      ELDD IF
Sbjct: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATP---ELDDEIF 65

Query: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
            GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV L+ 
Sbjct: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMW 125

Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
                   +++F+YTKMVSEMEKLVK MVFESYGV KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185

Query: 185 GEQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNG 244
           GE+NIGA+AHTDKSFFTILHQN                                 AWSNG
Sbjct: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAWSNG 245

Query: 245 RIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKE 279
           RIHSATHRV+IEG+KERYSTALFSYHKG+IEIP ELVDE+ PL+F+PFNHYGLL YFS  
Sbjct: 246 RIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTH 305

BLAST of Carg04251 vs. NCBI nr
Match: XP_016900413.1 (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo])

HSP 1 Score: 332.0 bits (850), Expect = 1.9e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0

Query: 79  MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
           MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+         ++LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
           +K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
           FFTILHQ    NG                              AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
           G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

BLAST of Carg04251 vs. TAIR10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 236.9 bits (603), Expect = 1.5e-62
Identity = 125/311 (40.19%), Postives = 180/311 (57.88%), Query Frame = 0

Query: 9   PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
           P++P ++FS E+L+P +  W  T  ++R ALEEYGCF+ + ++  +  ++L D +F  L 
Sbjct: 3   PKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD--KTPLDLLDRVFGSLV 62

Query: 69  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
            LFDLP QTKMKN  DKP +G++  + P  LPLHES GI+NA S E   SF  L+     
Sbjct: 63  DLFDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGN 122

Query: 129 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 188
               + L+ Y +  +E++++V RMVF+SY V KYYD +I STTYLLR +K R P      
Sbjct: 123 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 182

Query: 189 IGAEAHTDKSFFTILHQ---NG------------------------------AWSNGRIH 248
           +G   HTDKSF TILHQ   NG                              AWSN R+ 
Sbjct: 183 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVW 242

Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 278
           S  H+V++ G  +RYS  +F+++ G +++P+EL+D Q PL + PF+H GLL ++  +   
Sbjct: 243 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 302

BLAST of Carg04251 vs. TAIR10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 221.5 bits (563), Expect = 6.6e-58
Identity = 116/311 (37.30%), Postives = 173/311 (55.63%), Query Frame = 0

Query: 10  EVPVINF-SIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 69
           +VP+++  S + L+P ++ W         ALEEYGCFLA Y+   +Q   LDD+IF   E
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQ---LDDSIFAAAE 68

Query: 70  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 129
           +LFDLP +TK KNV++KPYHG++      V+PLHE  G++   + E  + F  L+     
Sbjct: 69  ELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGN 128

Query: 130 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 189
                 +  ++  V+E+++LV RM+FE+YGV K+Y+SH+ S TYLL+ +KY  P      
Sbjct: 129 DRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISM 188

Query: 190 IGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIHS 249
                HTDK+F +ILHQN                                  WSN RI S
Sbjct: 189 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRS 248

Query: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 279
             HRV +EG K RY+  LFS+   ++ IP+ELVD++ PL + PF++  L+ +++ +E  +
Sbjct: 249 CEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGRE 308

BLAST of Carg04251 vs. TAIR10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 212.6 bits (540), Expect = 3.1e-55
Identity = 119/322 (36.96%), Postives = 180/322 (55.90%), Query Frame = 0

Query: 1   MGFRSSTKP-EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVEL 60
           MG  +   P  +PVI+FS +NL+P   +W  T   V+ AL++YG F A ++       EL
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFD---RIPFEL 60

Query: 61  DDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESF 120
             ++F  LE+LFDLPLQTK++NVS KP+HG++      ++PL+ES GI+++   E V++F
Sbjct: 61  RKSVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAF 120

Query: 121 ---------VKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY 180
                    +     + +++K +SE++  ++RM+ ES+G+ KY D H+ ST YLLR MKY
Sbjct: 121 TEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKY 180

Query: 181 RIPEMGEQNIGAEAHTDKSFFTILHQNG-------------------------------- 240
           + P+  E  +G  AHTDK+  TIL+QN                                 
Sbjct: 181 KGPDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLY 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGL 279
           A  NGR+HS  HRV++ G++ RYS  LFS  K   ++  PDELVDE+ P  F PF+H   
Sbjct: 241 ALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEF 300

BLAST of Carg04251 vs. TAIR10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 113/316 (35.76%), Postives = 163/316 (51.58%), Query Frame = 0

Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           E+P+IN S +NL+  +  W  T   VR A+E +G F+AEY N       L  +I    ++
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNN---FPTGLHQSILEAAKE 67

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI------ 129
           L DLP++ K+KN + K  HG++   +    P+HE  GI+     +    F +L+      
Sbjct: 68  LLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHD 127

Query: 130 ------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGE 189
                 + + AY KM +E+E+LV RM+FESY V KY + +I  T YLLR +KYR    GE
Sbjct: 128 DNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGE 187

Query: 190 QNIGAEAHTDKSFFTILHQNG---------------------------------AWSNGR 249
            N    +HTDKSF +ILHQN                                  AWSN R
Sbjct: 188 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSNDR 247

Query: 250 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           I +  H+V +E  + RYS   FS+ +G+I  P+E+VD+  PL +NPF+H GLL Y+   E
Sbjct: 248 IKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLE 307

BLAST of Carg04251 vs. TAIR10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0

Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
                 +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

BLAST of Carg04251 vs. Swiss-Prot
Match: sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.8e-47
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0

Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
                 +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

BLAST of Carg04251 vs. Swiss-Prot
Match: sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.5e-47
Identity = 111/303 (36.63%), Postives = 162/303 (53.47%), Query Frame = 0

Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI++A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY-----RIPE 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 MGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SN 249
                +G  +HTDK+  TILHQ           +  W                      N
Sbjct: 193 XXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLN 252

Query: 250 GRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYF 264
           GR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 306

BLAST of Carg04251 vs. Swiss-Prot
Match: sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.5e-47
Identity = 111/302 (36.75%), Postives = 161/302 (53.31%), Query Frame = 0

Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 65

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS K +HG+L H       L+ES GI +A   E V  F +        
Sbjct: 66  LFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 125

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY----RIPEM 189
             + + +  +++ + E++ +V+RM+ ES+G+ KY D H+ ST YL R MKY         
Sbjct: 126 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTXXXXXXXX 185

Query: 190 GEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SNG 249
               +G  +HTDK+  TILHQ           +  W                      NG
Sbjct: 186 XXTKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNG 245

Query: 250 RIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYFS 264
           R+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 298

BLAST of Carg04251 vs. Swiss-Prot
Match: sp|Q945B4|AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.7e-34
Identity = 114/412 (27.67%), Postives = 169/412 (41.02%), Query Frame = 0

Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P++P+I  S + L+P S+KWV+  + VR ALE+YGCF A+ +   +  +EL  ++  
Sbjct: 3   SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV----- 125
            +++LF LP + K +NV  KP+ G+  H       L ESFGI++A   E    F      
Sbjct: 63  AMQELFALPTEAKQRNVCPKPFAGYFSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122

Query: 126 ---KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------- 185
              K I+ +  Y + ++E++ +V+R++ ESYG+  + D H+ ST Y +R MKY       
Sbjct: 123 EGNKSIKMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDND 182

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 183 ITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 246 -----------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN---- 279
                                      +G  E N+ AE    +HTDKS   I++Q+    
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDG 302

BLAST of Carg04251 vs. Swiss-Prot
Match: sp|Q9ZTA1|AOP3C_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.1e-33
Identity = 118/416 (28.37%), Postives = 171/416 (41.11%), Query Frame = 0

Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P++P+I  S + L+P S+KWV+  + VR ALE+YGCF A+ +   +  +EL  ++  
Sbjct: 3   SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGI------ENAASF------ 125
            +++LF LP + K +NV  KP+ G+L H       L ESFGI      E A  F      
Sbjct: 63  AMQELFALPTEAKQRNVCPKPFTGYLSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122

Query: 126 EGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY--- 185
           EG +S  K+IQ    Y + ++E++ +V+R++ ESYG+  + D H+ ST Y +R MKY   
Sbjct: 123 EGNKSISKMIQ---LYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIAR 182

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 183 PDNDITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 246 ---------------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN 279
                                          +G  E N+ AE    +HTDKS   I++Q+
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLTGIIYQH 302

BLAST of Carg04251 vs. TrEMBL
Match: tr|A0A1S4DWR1|A0A1S4DWR1_CUCME (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0

Query: 79  MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
           MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+         ++LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
           +K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
           FFTILHQ    NG                              AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
           G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

BLAST of Carg04251 vs. TrEMBL
Match: tr|W9STP2|W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.8e-79
Identity = 156/312 (50.00%), Postives = 200/312 (64.10%), Query Frame = 0

Query: 9   PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
           P++P I+FS  NL P ++ W   C  VR ALEEYGCF+A Y+   +Q   L  ++F+ L+
Sbjct: 7   PKIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQ---LRTSMFSELK 66

Query: 69  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
           +LFDLP + KMKNVSDKPY G++ +  P + PLHES GIENA + EG ESF  +I     
Sbjct: 67  ELFDLPTEIKMKNVSDKPYFGYIGNH-PSIPPLHESMGIENATTLEGGESFTNIIWPKGG 126

Query: 129 -----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQ 188
                + + +Y+++VSE+EK+VKRMVF+SYGV KYYDSH+ STT+LL+ +KYR+PEM E 
Sbjct: 127 NNHFCETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNES 186

Query: 189 NIGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIH 248
           +IG   HTDKSF T+LHQN                                 AW+NGRIH
Sbjct: 187 SIGCAPHTDKSFITVLHQNEVNGLEIETRDGDWIQFEPSPSSFLVLAGDAFLAWTNGRIH 246

Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 279
           SA HRV++ G+K RYS  LFSYH G IEIPDELVDEQ P +F  F+HYGLL ++      
Sbjct: 247 SAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAAP 306

BLAST of Carg04251 vs. TrEMBL
Match: tr|A5AJI0|A5AJI0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.4e-78
Identity = 154/314 (49.04%), Postives = 199/314 (63.38%), Query Frame = 0

Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P +PV+NFS   L+P +  W+  C  V  ALEEYGCF+A Y+   +   ELD+  F 
Sbjct: 3   SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
            LE+LFDLP +TK+KN SDKPY G++      ++PLHES GIE+A + +GVESF  ++  
Sbjct: 63  QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122

Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
                  + + +Y+K+VSE+E++VK+MVFESY   KYYDSHI STTYLLR +KYR P+  
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182

Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
           E  IG   HTDKSF T+LHQN                                 AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242

Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           IHS  HRVI+ G+K RYS  LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++   +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302

BLAST of Carg04251 vs. TrEMBL
Match: tr|D7U0Q7|D7U0Q7_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.3e-78
Identity = 153/314 (48.73%), Postives = 198/314 (63.06%), Query Frame = 0

Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P +PV+NFS   L+P +  W+  C  V  ALEEYGCF+A Y+   +   ELD+  F 
Sbjct: 3   SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
            LE+LFDLP +TK+KN SDKPY G++      ++PLHES GIE+A + +GVESF  ++  
Sbjct: 63  QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122

Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
                  + + +Y+K+VSE+E++VK+MVFESY   KYYDSHI STTYLLR +KYR P+  
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182

Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
           E  IG   HTDKSF T+LHQN                                 AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242

Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           IHS  HRVI+ G+K RYS  LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++   +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302

BLAST of Carg04251 vs. TrEMBL
Match: tr|B9RUX7|B9RUX7_RICCO (Gibberellin 20 oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_0896520 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.0e-73
Identity = 146/316 (46.20%), Postives = 195/316 (61.71%), Query Frame = 0

Query: 5   SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
           S   P++PVI+FS ENL+P ++ WV TC +V  ALEEYGCF+A YE   +  +EL  + +
Sbjct: 3   SRIPPKIPVIDFSQENLKPGTSSWVSTCKNVLRALEEYGCFIAVYE---KIPLELQSSFY 62

Query: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
           N L  LFDLP+ TK KNVS  PY+G++ ++ PFV PL+E  GI NAA+ EG   F  ++ 
Sbjct: 63  NKLITLFDLPIDTKRKNVSAIPYYGYVGNQ-PFVPPLYEGMGINNAATMEGTLDFTNIMW 122

Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
                   + +F+Y+K V+E+E++V RMVFE YGV   YD+    T YL R MKYR P  
Sbjct: 123 PNGDDDFCKTVFSYSKWVAELEQMVTRMVFEGYGVQYSYDTQQERTNYLFRMMKYRAPTE 182

Query: 185 GEQNIGAEAHTDKSFFTILHQNG---------------------------------AWSN 244
           GE ++G + HTDK F T+LHQN                                  AWSN
Sbjct: 183 GESDLGCDVHTDKGFITVLHQNEVSGLEVQTKDGQWVCFEPSSPSSFIVMAADALLAWSN 242

Query: 245 GRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSK 279
           GRIH+A HRV++ GSK RYS  LFSY KG+ E+P+EL+DE+ PL+F PF+++GLL YF  
Sbjct: 243 GRIHAAFHRVVMRGSKARYSVGLFSYLKGITEVPEELIDEEHPLQFKPFDNFGLLHYFYT 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948698.16.4e-15285.94probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata][more]
XP_023525673.11.8e-14984.062-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo][more]
XP_022997882.11.0e-14482.502-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima][more]
XP_022153580.11.4e-11467.302-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia][more]
XP_016900413.11.9e-8768.31PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G52790.11.5e-6240.192-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52800.16.6e-5837.302-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G52820.13.1e-5536.962-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT1G15540.12.1e-4835.762-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT4G03070.12.1e-4836.512-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q9ZTA3|AOP1C_ARATH3.8e-4736.51Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37... [more]
sp|Q945B6|AOP1L_ARATH6.5e-4736.63Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=... [more]
sp|Q944Z5|AOP1V_ARATH8.5e-4736.75Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
sp|Q945B4|AOP3L_ARATH3.7e-3427.672-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
sp|Q9ZTA1|AOP3C_ARATH4.1e-3328.372-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP... [more]
Match NameE-valueIdentityDescription
tr|A0A1S4DWR1|A0A1S4DWR1_CUCME1.3e-8768.312-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
tr|W9STP2|W9STP2_9ROSA4.8e-7950.00Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=... [more]
tr|A5AJI0|A5AJI0_VITVI1.4e-7849.04Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1[more]
tr|D7U0Q7|D7U0Q7_VITVI5.3e-7848.73Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=... [more]
tr|B9RUX7|B9RUX7_RICCO3.0e-7346.20Gibberellin 20 oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_0896520 PE=... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR026992DIOX_N
IPR027443IPNS-like
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04251-RACarg04251-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 199..263
e-value: 7.5E-10
score: 40.5
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 2..198
e-value: 2.7E-18
score: 68.5
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 11..90
e-value: 8.8E-7
score: 29.6
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 7..197
coord: 199..277
NoneNo IPR availablePANTHERPTHR10209:SF1822-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 7..197
coord: 199..277
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 8..261

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Carg04251Cla015406Watermelon (97103) v1carwmB0187
Carg04251Cla012687Watermelon (97103) v1carwmB0192
Carg04251Csa3G555690Cucumber (Chinese Long) v2carcuB0221
Carg04251Csa7G434950Cucumber (Chinese Long) v2carcuB0229
Carg04251MELO3C023381Melon (DHL92) v3.5.1carmeB0198
Carg04251ClCG09G002470Watermelon (Charleston Gray)carwcgB0190
Carg04251ClCG07G012930Watermelon (Charleston Gray)carwcgB0199
Carg04251CSPI03G25170Wild cucumber (PI 183967)carcpiB0228
Carg04251CSPI07G21000Wild cucumber (PI 183967)carcpiB0236
Carg04251CmaCh17G008320Cucurbita maxima (Rimu)carcmaB0277
Carg04251CmaCh01G001210Cucurbita maxima (Rimu)carcmaB0278
Carg04251CmoCh17G008020Cucurbita moschata (Rifu)carcmoB0284
Carg04251CmoCh01G001270Cucurbita moschata (Rifu)carcmoB0285
Carg04251Lsi07G004900Bottle gourd (USVL1VR-Ls)carlsiB182
Carg04251Lsi02G027510Bottle gourd (USVL1VR-Ls)carlsiB176
Carg04251Cp4.1LG12g07740Cucurbita pepo (Zucchini)carcpeB0280
Carg04251Cp4.1LG02g14620Cucurbita pepo (Zucchini)carcpeB0285
Carg04251CsGy3G023650Cucumber (Gy14) v2carcgybB0210
Carg04251CsGy7G019260Cucumber (Gy14) v2carcgybB0218
Carg04251MELO3C030548.2Melon (DHL92) v3.6.1carmedB0209
Carg04251MELO3C023381.2Melon (DHL92) v3.6.1carmedB0203
Carg04251CsaV3_7G032850Cucumber (Chinese Long) v3carcucB0233
Carg04251CsaV3_3G026700Cucumber (Chinese Long) v3carcucB0225
Carg04251Cla97C07G139750Watermelon (97103) v2carwmbB0205
Carg04251Cla97C09G164610Watermelon (97103) v2carwmbB0209
Carg04251Bhi01G002663Wax gourdcarwgoB0249
Carg04251Bhi09G000062Wax gourdcarwgoB0253
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Carg04251Carg09558Silver-seed gourdcarcarB240