BLAST of Carg04251 vs. NCBI nr
Match:
XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])
HSP 1 Score: 546.2 bits (1406), Expect = 6.4e-152
Identity = 275/320 (85.94%), Postives = 276/320 (86.25%), Query Frame = 0
Query: 1 MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
MGFRSSTKPEVPVINFSIENL PKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1 MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
Query: 61 DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61 DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
KL+ QDLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180
Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
IPEMGEQNIGAEAHTDKSFFTILHQNG
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACL 240
Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300
BLAST of Carg04251 vs. NCBI nr
Match:
XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 538.1 bits (1385), Expect = 1.8e-149
Identity = 269/320 (84.06%), Postives = 276/320 (86.25%), Query Frame = 0
Query: 1 MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
MGFRSST PEVPVI+FSIENLRPKSTKWVETC+HVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1 MGFRSSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELD 60
Query: 61 DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61 DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
KL+ +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180
Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
IPEMGEQNIGAEAHTDKSFFTILHQNG
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240
Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIP+ELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHYGLLG 300
BLAST of Carg04251 vs. NCBI nr
Match:
XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])
HSP 1 Score: 522.3 bits (1344), Expect = 1.0e-144
Identity = 264/320 (82.50%), Postives = 270/320 (84.38%), Query Frame = 0
Query: 1 MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
MGFRSS KPEVP INFSIENLRPKSTKWVETC+HVRAALEEYGCFLAE EN+ VELD
Sbjct: 1 MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECENN----VELD 60
Query: 61 DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61 DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
KL+ +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180
Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
IPEMGEQNIGAEAHTDKSFFTILHQNG
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240
Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
AWSNGRIHSATHRV+IEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300
BLAST of Carg04251 vs. NCBI nr
Match:
XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])
HSP 1 Score: 422.2 bits (1084), Expect = 1.4e-114
Identity = 212/315 (67.30%), Postives = 240/315 (76.19%), Query Frame = 0
Query: 5 SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
S K ++PVIN SIEN+ P+ST W+E C+H+RAALEEYGCFL E+ + ELDD IF
Sbjct: 6 SLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATP---ELDDEIF 65
Query: 65 NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV L+
Sbjct: 66 KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMW 125
Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
+++F+YTKMVSEMEKLVK MVFESYGV KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185
Query: 185 GEQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNG 244
GE+NIGA+AHTDKSFFTILHQN AWSNG
Sbjct: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAWSNG 245
Query: 245 RIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKE 279
RIHSATHRV+IEG+KERYSTALFSYHKG+IEIP ELVDE+ PL+F+PFNHYGLL YFS
Sbjct: 246 RIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTH 305
BLAST of Carg04251 vs. NCBI nr
Match:
XP_016900413.1 (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo])
HSP 1 Score: 332.0 bits (850), Expect = 1.9e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0
Query: 79 MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+ ++LFAY
Sbjct: 1 MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60
Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
+K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61 SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120
Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
FFTILHQ NG AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180
Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240
BLAST of Carg04251 vs. TAIR10
Match:
AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 236.9 bits (603), Expect = 1.5e-62
Identity = 125/311 (40.19%), Postives = 180/311 (57.88%), Query Frame = 0
Query: 9 PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
P++P ++FS E+L+P + W T ++R ALEEYGCF+ + ++ + ++L D +F L
Sbjct: 3 PKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD--KTPLDLLDRVFGSLV 62
Query: 69 KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
LFDLP QTKMKN DKP +G++ + P LPLHES GI+NA S E SF L+
Sbjct: 63 DLFDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGN 122
Query: 129 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 188
+ L+ Y + +E++++V RMVF+SY V KYYD +I STTYLLR +K R P
Sbjct: 123 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 182
Query: 189 IGAEAHTDKSFFTILHQ---NG------------------------------AWSNGRIH 248
+G HTDKSF TILHQ NG AWSN R+
Sbjct: 183 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVW 242
Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 278
S H+V++ G +RYS +F+++ G +++P+EL+D Q PL + PF+H GLL ++ +
Sbjct: 243 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 302
BLAST of Carg04251 vs. TAIR10
Match:
AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 221.5 bits (563), Expect = 6.6e-58
Identity = 116/311 (37.30%), Postives = 173/311 (55.63%), Query Frame = 0
Query: 10 EVPVINF-SIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 69
+VP+++ S + L+P ++ W ALEEYGCFLA Y+ +Q LDD+IF E
Sbjct: 9 KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQ---LDDSIFAAAE 68
Query: 70 KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 129
+LFDLP +TK KNV++KPYHG++ V+PLHE G++ + E + F L+
Sbjct: 69 ELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGN 128
Query: 130 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 189
+ ++ V+E+++LV RM+FE+YGV K+Y+SH+ S TYLL+ +KY P
Sbjct: 129 DRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISM 188
Query: 190 IGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIHS 249
HTDK+F +ILHQN WSN RI S
Sbjct: 189 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRS 248
Query: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 279
HRV +EG K RY+ LFS+ ++ IP+ELVD++ PL + PF++ L+ +++ +E +
Sbjct: 249 CEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGRE 308
BLAST of Carg04251 vs. TAIR10
Match:
AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 212.6 bits (540), Expect = 3.1e-55
Identity = 119/322 (36.96%), Postives = 180/322 (55.90%), Query Frame = 0
Query: 1 MGFRSSTKP-EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVEL 60
MG + P +PVI+FS +NL+P +W T V+ AL++YG F A ++ EL
Sbjct: 1 MGSETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFD---RIPFEL 60
Query: 61 DDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESF 120
++F LE+LFDLPLQTK++NVS KP+HG++ ++PL+ES GI+++ E V++F
Sbjct: 61 RKSVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAF 120
Query: 121 ---------VKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY 180
+ + +++K +SE++ ++RM+ ES+G+ KY D H+ ST YLLR MKY
Sbjct: 121 TEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKY 180
Query: 181 RIPEMGEQNIGAEAHTDKSFFTILHQNG-------------------------------- 240
+ P+ E +G AHTDK+ TIL+QN
Sbjct: 181 KGPDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLY 240
Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGL 279
A NGR+HS HRV++ G++ RYS LFS K ++ PDELVDE+ P F PF+H
Sbjct: 241 ALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEF 300
BLAST of Carg04251 vs. TAIR10
Match:
AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 113/316 (35.76%), Postives = 163/316 (51.58%), Query Frame = 0
Query: 10 EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
E+P+IN S +NL+ + W T VR A+E +G F+AEY N L +I ++
Sbjct: 8 ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNN---FPTGLHQSILEAAKE 67
Query: 70 LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI------ 129
L DLP++ K+KN + K HG++ + P+HE GI+ + F +L+
Sbjct: 68 LLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHD 127
Query: 130 ------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGE 189
+ + AY KM +E+E+LV RM+FESY V KY + +I T YLLR +KYR GE
Sbjct: 128 DNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGE 187
Query: 190 QNIGAEAHTDKSFFTILHQNG---------------------------------AWSNGR 249
N +HTDKSF +ILHQN AWSN R
Sbjct: 188 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSNDR 247
Query: 250 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
I + H+V +E + RYS FS+ +G+I P+E+VD+ PL +NPF+H GLL Y+ E
Sbjct: 248 IKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLE 307
BLAST of Carg04251 vs. TAIR10
Match:
AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)
HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0
Query: 10 EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
++PVI+FS +NL+P S+KW E V ALE+YGCF A ++ + VEL+ ++F +E
Sbjct: 13 QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72
Query: 70 LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
LF+LP+ TK +NVS KP+HG+L H L+ES GI +A E V F +
Sbjct: 73 LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132
Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
+ + + +++ + E++ +V+RM+ ES+G+ Y D H+ ST YL R MKY
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192
Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
+G +HTDK+ TILHQ + W
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252
Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
NGR+HS HRVI+ G K RYST LFS K +I+ P+ELVD++ P F PF + L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307
BLAST of Carg04251 vs. Swiss-Prot
Match:
sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)
HSP 1 Score: 189.9 bits (481), Expect = 3.8e-47
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0
Query: 10 EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
++PVI+FS +NL+P S+KW E V ALE+YGCF A ++ + VEL+ ++F +E
Sbjct: 13 QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72
Query: 70 LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
LF+LP+ TK +NVS KP+HG+L H L+ES GI +A E V F +
Sbjct: 73 LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132
Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
+ + + +++ + E++ +V+RM+ ES+G+ Y D H+ ST YL R MKY
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192
Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
+G +HTDK+ TILHQ + W
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252
Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
NGR+HS HRVI+ G K RYST LFS K +I+ P+ELVD++ P F PF + L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307
BLAST of Carg04251 vs. Swiss-Prot
Match:
sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)
HSP 1 Score: 189.1 bits (479), Expect = 6.5e-47
Identity = 111/303 (36.63%), Postives = 162/303 (53.47%), Query Frame = 0
Query: 10 EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
++PVI+FS +NL+P S+KW E V ALE+YGCF A ++ + VEL+ ++F +E
Sbjct: 13 QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFD---KLSVELNRSVFEAMED 72
Query: 70 LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
LF+LP+ TK +NVS KP+HG+L H L+ES GI++A E V F +
Sbjct: 73 LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGN 132
Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY-----RIPE 189
+ + + +++ + E++ +V+RM+ ES+G+ Y D H+ ST YL R MKY
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192
Query: 190 MGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SN 249
+G +HTDK+ TILHQ + W N
Sbjct: 193 XXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLN 252
Query: 250 GRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYF 264
GR+HS HRVI+ G K RYST LFS K +I+ P+ELVD++ P F PF + L +F
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 306
BLAST of Carg04251 vs. Swiss-Prot
Match:
sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)
HSP 1 Score: 188.7 bits (478), Expect = 8.5e-47
Identity = 111/302 (36.75%), Postives = 161/302 (53.31%), Query Frame = 0
Query: 10 EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
++PVI+FS +NL+P S+KW E V ALE+YGCF A ++ + VEL+ ++F +E
Sbjct: 6 QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 65
Query: 70 LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
LF+LP+ TK +NVS K +HG+L H L+ES GI +A E V F +
Sbjct: 66 LFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 125
Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY----RIPEM 189
+ + + +++ + E++ +V+RM+ ES+G+ KY D H+ ST YL R MKY
Sbjct: 126 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTXXXXXXXX 185
Query: 190 GEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SNG 249
+G +HTDK+ TILHQ + W NG
Sbjct: 186 XXTKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNG 245
Query: 250 RIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYFS 264
R+HS HRVI+ G K RYST LFS K +I+ P+ELVD++ P F PF + L +F
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 298
BLAST of Carg04251 vs. Swiss-Prot
Match:
sp|Q945B4|AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)
HSP 1 Score: 146.7 bits (369), Expect = 3.7e-34
Identity = 114/412 (27.67%), Postives = 169/412 (41.02%), Query Frame = 0
Query: 6 STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
S P++P+I S + L+P S+KWV+ + VR ALE+YGCF A+ + + +EL ++
Sbjct: 3 SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62
Query: 66 GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV----- 125
+++LF LP + K +NV KP+ G+ H L ESFGI++A E F
Sbjct: 63 AMQELFALPTEAKQRNVCPKPFAGYFSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122
Query: 126 ---KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------- 185
K I+ + Y + ++E++ +V+R++ ESYG+ + D H+ ST Y +R MKY
Sbjct: 123 EGNKSIKMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDND 182
Query: 186 ------------------------------------------------------------ 245
Sbjct: 183 ITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242
Query: 246 -----------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN---- 279
+G E N+ AE +HTDKS I++Q+
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDG 302
BLAST of Carg04251 vs. Swiss-Prot
Match:
sp|Q9ZTA1|AOP3C_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)
HSP 1 Score: 143.3 bits (360), Expect = 4.1e-33
Identity = 118/416 (28.37%), Postives = 171/416 (41.11%), Query Frame = 0
Query: 6 STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
S P++P+I S + L+P S+KWV+ + VR ALE+YGCF A+ + + +EL ++
Sbjct: 3 SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62
Query: 66 GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGI------ENAASF------ 125
+++LF LP + K +NV KP+ G+L H L ESFGI E A F
Sbjct: 63 AMQELFALPTEAKQRNVCPKPFTGYLSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122
Query: 126 EGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY--- 185
EG +S K+IQ Y + ++E++ +V+R++ ESYG+ + D H+ ST Y +R MKY
Sbjct: 123 EGNKSISKMIQ---LYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIAR 182
Query: 186 ------------------------------------------------------------ 245
Sbjct: 183 PDNDITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242
Query: 246 ---------------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN 279
+G E N+ AE +HTDKS I++Q+
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLTGIIYQH 302
BLAST of Carg04251 vs. TrEMBL
Match:
tr|A0A1S4DWR1|A0A1S4DWR1_CUCME (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)
HSP 1 Score: 332.0 bits (850), Expect = 1.3e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0
Query: 79 MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+ ++LFAY
Sbjct: 1 MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60
Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
+K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61 SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120
Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
FFTILHQ NG AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180
Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240
BLAST of Carg04251 vs. TrEMBL
Match:
tr|W9STP2|W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=3 SV=1)
HSP 1 Score: 303.5 bits (776), Expect = 4.8e-79
Identity = 156/312 (50.00%), Postives = 200/312 (64.10%), Query Frame = 0
Query: 9 PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
P++P I+FS NL P ++ W C VR ALEEYGCF+A Y+ +Q L ++F+ L+
Sbjct: 7 PKIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQ---LRTSMFSELK 66
Query: 69 KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
+LFDLP + KMKNVSDKPY G++ + P + PLHES GIENA + EG ESF +I
Sbjct: 67 ELFDLPTEIKMKNVSDKPYFGYIGNH-PSIPPLHESMGIENATTLEGGESFTNIIWPKGG 126
Query: 129 -----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQ 188
+ + +Y+++VSE+EK+VKRMVF+SYGV KYYDSH+ STT+LL+ +KYR+PEM E
Sbjct: 127 NNHFCETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNES 186
Query: 189 NIGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIH 248
+IG HTDKSF T+LHQN AW+NGRIH
Sbjct: 187 SIGCAPHTDKSFITVLHQNEVNGLEIETRDGDWIQFEPSPSSFLVLAGDAFLAWTNGRIH 246
Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 279
SA HRV++ G+K RYS LFSYH G IEIPDELVDEQ P +F F+HYGLL ++
Sbjct: 247 SAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAAP 306
BLAST of Carg04251 vs. TrEMBL
Match:
tr|A5AJI0|A5AJI0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1)
HSP 1 Score: 302.0 bits (772), Expect = 1.4e-78
Identity = 154/314 (49.04%), Postives = 199/314 (63.38%), Query Frame = 0
Query: 6 STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
S P +PV+NFS L+P + W+ C V ALEEYGCF+A Y+ + ELD+ F
Sbjct: 3 SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62
Query: 66 GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
LE+LFDLP +TK+KN SDKPY G++ ++PLHES GIE+A + +GVESF ++
Sbjct: 63 QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122
Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
+ + +Y+K+VSE+E++VK+MVFESY KYYDSHI STTYLLR +KYR P+
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182
Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
E IG HTDKSF T+LHQN AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242
Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
IHS HRVI+ G+K RYS LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++ +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302
BLAST of Carg04251 vs. TrEMBL
Match:
tr|D7U0Q7|D7U0Q7_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=1)
HSP 1 Score: 300.1 bits (767), Expect = 5.3e-78
Identity = 153/314 (48.73%), Postives = 198/314 (63.06%), Query Frame = 0
Query: 6 STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
S P +PV+NFS L+P + W+ C V ALEEYGCF+A Y+ + ELD+ F
Sbjct: 3 SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62
Query: 66 GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
LE+LFDLP +TK+KN SDKPY G++ ++PLHES GIE+A + +GVESF ++
Sbjct: 63 QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122
Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
+ + +Y+K+VSE+E++VK+MVFESY KYYDSHI STTYLLR +KYR P+
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182
Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
E IG HTDKSF T+LHQN AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242
Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
IHS HRVI+ G+K RYS LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++ +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302
BLAST of Carg04251 vs. TrEMBL
Match:
tr|B9RUX7|B9RUX7_RICCO (Gibberellin 20 oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_0896520 PE=3 SV=1)
HSP 1 Score: 284.3 bits (726), Expect = 3.0e-73
Identity = 146/316 (46.20%), Postives = 195/316 (61.71%), Query Frame = 0
Query: 5 SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
S P++PVI+FS ENL+P ++ WV TC +V ALEEYGCF+A YE + +EL + +
Sbjct: 3 SRIPPKIPVIDFSQENLKPGTSSWVSTCKNVLRALEEYGCFIAVYE---KIPLELQSSFY 62
Query: 65 NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
N L LFDLP+ TK KNVS PY+G++ ++ PFV PL+E GI NAA+ EG F ++
Sbjct: 63 NKLITLFDLPIDTKRKNVSAIPYYGYVGNQ-PFVPPLYEGMGINNAATMEGTLDFTNIMW 122
Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
+ +F+Y+K V+E+E++V RMVFE YGV YD+ T YL R MKYR P
Sbjct: 123 PNGDDDFCKTVFSYSKWVAELEQMVTRMVFEGYGVQYSYDTQQERTNYLFRMMKYRAPTE 182
Query: 185 GEQNIGAEAHTDKSFFTILHQNG---------------------------------AWSN 244
GE ++G + HTDK F T+LHQN AWSN
Sbjct: 183 GESDLGCDVHTDKGFITVLHQNEVSGLEVQTKDGQWVCFEPSSPSSFIVMAADALLAWSN 242
Query: 245 GRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSK 279
GRIH+A HRV++ GSK RYS LFSY KG+ E+P+EL+DE+ PL+F PF+++GLL YF
Sbjct: 243 GRIHAAFHRVVMRGSKARYSVGLFSYLKGITEVPEELIDEEHPLQFKPFDNFGLLHYFYT 302
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022948698.1 | 6.4e-152 | 85.94 | probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata] | [more] |
XP_023525673.1 | 1.8e-149 | 84.06 | 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo] | [more] |
XP_022997882.1 | 1.0e-144 | 82.50 | 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima] | [more] |
XP_022153580.1 | 1.4e-114 | 67.30 | 2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia] | [more] |
XP_016900413.1 | 1.9e-87 | 68.31 | PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo] | [more] |
Match Name | E-value | Identity | Description | |
AT1G52790.1 | 1.5e-62 | 40.19 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
AT1G52800.1 | 6.6e-58 | 37.30 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
AT1G52820.1 | 3.1e-55 | 36.96 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
AT1G15540.1 | 2.1e-48 | 35.76 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |
AT4G03070.1 | 2.1e-48 | 36.51 | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein | [more] |