Carg04251-RA (mRNA) Silver-seed gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Carg04251-RA
Type	mRNA
Organism	Cucurbita argyrosperma (Silver-seed gourd)
Description	2-oxoglutarate-dependent dioxygenase
Location	Cucurbita_argyrosperma_scaffold_044 : 1056541 .. 1058177 (+)
Sequence length	837

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATGTGGCCTTCTGGAAATCATCATTTCTGGTAAACTTTGTATGAATTACATGGTTGTCTATTTTGGGTGTAAATCTTTAAAATCTTACTTGGAAATGATTTTGTTGGACAGTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGTCAATGGCTTGGAAATCAAAGCAAAAAAGGATGATCGTTGGATTAGACTTCAATTCAATCCAAATTCCTTCTTGGTCATGGCTGGAGATGCTTGTTTGGTATACCTCTTACTTGCACTTTCATCCATACTTCAATTATACACACACATATACGGTAATAGTCCAAGTCCACCGCTAACAGATATTGTTCTGTTTGGACTTCCCCTCAAGATTTTTAAAATGCTTATGTTAGACAGAGGTTTCCACACTCGATGCGGGATCTCACAATCCACTCCCCTTCGGGGCCAACGTTCTTGCTGACACTCGTTCCCTTCTCTAATCGATGTGGGACCCCCAATCCACCCTCTTTGGGGTCCAACGTCCTTCCTAACACATCACCCGATGCTTAGCTTTGATATCATTTGTAACAGCCCAAACTCACTACTAGTAGATATTGTTTTCTTTAGGCTTTCCCTAACTCACTACTAGTAGATATTGCCTTCTTTAGGCTTTCCCTTTTGGTCTTCTCCTCAAAGTTTTTAAAGACTCGTCTACTGGGGAGAGATTTCCACACCCTTATAAAGAATGATTCGTTTTCTTCCTAACGTGAGATCTCACATGTAGTTATCAATTGTTTATCCCAAAGATTGATGATTTGAATACTTGAATTAAGTATTGAATGGTAAGAACAAAATTTGATTTTGATGGATAGGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

mRNA sequence

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Coding sequence (CDS)

ATGGGGTTTCGTTCTTCCACAAAGCCTGAGGTTCCAGTCATCAACTTCTCCATTGAAAACCTTCGTCCTAAAAGCACCAAATGGGTAGAAACATGCACCCATGTGAGGGCTGCTCTTGAAGAATATGGGTGTTTCTTGGCTGAGTATGAGAATGATGTTGAGCAAGAGGTTGAGCTTGATGATGCCATTTTCAATGGCTTGGAAAAGCTTTTTGATTTGCCTTTACAAACTAAAATGAAGAATGTTTCTGATAAGCCTTACCATGGCTTTTTGGACCACAGGGCTCCCTTTGTTCTTCCTCTTCATGAAAGCTTTGGCATTGAAAATGCAGCGAGTTTTGAAGGTGTTGAGTCCTTTGTGAAGCTCATTCAGGATTTGTTTGCATACACAAAGATGGTATCAGAGATGGAGAAGTTAGTGAAGAGGATGGTGTTTGAAAGCTATGGAGTGGGAAAGTACTATGATTCTCATATTGCTTCAACCACTTATTTGCTTAGAGGCATGAAATATAGAATCCCTGAGATGGGTGAACAGAATATTGGAGCAGAAGCTCACACAGACAAGAGTTTCTTCACTATTCTTCATCAAAATGGAGCATGGAGCAATGGGAGAATCCACTCAGCCACACATAGAGTTATAATAGAGGGCAGCAAGGAAAGATATTCAACAGCCCTATTTTCATACCACAAAGGAGTTATTGAGATTCCAGATGAGTTGGTGGACGAACAATTCCCCTTGAAATTCAACCCTTTTAACCATTATGGATTACTCGGTTATTTCTCCAAAGAAGAGTGCGAGAAAACGCCTTCCACTGCCGGAGCTTATTGTGGAGTTTGA

Protein sequence

MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKSFFTILHQNGAWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAYCGV

Homology

[Close]

BLAST of Carg04251-RA vs. NCBI nr
Match: XP_022948698.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata])

HSP 1 Score: 546.2 bits (1406), Expect = 6.4e-152
Identity = 275/320 (85.94%), Postives = 276/320 (86.25%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSSTKPEVPVINFSIENL PKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTKPEVPVINFSIENLGPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         QDLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCQDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNSNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251-RA vs. NCBI nr
Match: XP_023525673.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538.1 bits (1385), Expect = 1.8e-149
Identity = 269/320 (84.06%), Postives = 276/320 (86.25%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSST PEVPVI+FSIENLRPKSTKWVETC+HVRAALEEYGCFLAEYENDVEQEVELD
Sbjct: 1   MGFRSSTNPEVPVISFSIENLRPKSTKWVETCSHVRAALEEYGCFLAEYENDVEQEVELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIP+ELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPNELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251-RA vs. NCBI nr
Match: XP_022997882.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima])

HSP 1 Score: 522.3 bits (1344), Expect = 1.0e-144
Identity = 264/320 (82.50%), Postives = 270/320 (84.38%), Query Frame = 0


Query: 1   MGFRSSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELD 60
           MGFRSS KPEVP INFSIENLRPKSTKWVETC+HVRAALEEYGCFLAE EN+    VELD
Sbjct: 1   MGFRSSKKPEVPAINFSIENLRPKSTKWVETCSHVRAALEEYGCFLAECENN----VELD 60

Query: 61  DAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120
           DAIFNGLEKLFDLPL+TKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV
Sbjct: 61  DAIFNGLEKLFDLPLETKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV 120

Query: 121 KLI---------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYR 180
           KL+         +DLFAYTKMVSEMEKLVKRMVFESYGV KYYDSHIASTTYLLRGMKYR
Sbjct: 121 KLMWPSGNHHFCEDLFAYTKMVSEMEKLVKRMVFESYGVEKYYDSHIASTTYLLRGMKYR 180

Query: 181 IPEMGEQNIGAEAHTDKSFFTILHQNG--------------------------------- 240
           IPEMGEQNIGAEAHTDKSFFTILHQNG                                 
Sbjct: 181 IPEMGEQNIGAEAHTDKSFFTILHQNGVNGLEIKAKKDDHWIGLQFNPNSFLVMAGDACL 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 279
           AWSNGRIHSATHRV+IEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG
Sbjct: 241 AWSNGRIHSATHRVLIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLG 300

[Close]

BLAST of Carg04251-RA vs. NCBI nr
Match: XP_022153580.1 (2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 1.4e-114
Identity = 212/315 (67.30%), Postives = 240/315 (76.19%), Query Frame = 0


Query: 5   SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
           S  K ++PVIN SIEN+ P+ST W+E C+H+RAALEEYGCFL E+ +      ELDD IF
Sbjct: 6   SLAKAKLPVINLSIENVSPESTNWLEICSHLRAALEEYGCFLGEFGSATP---ELDDEIF 65

Query: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
            GLE+LFDLPL+TKMKNVSDKPYHGFLDHR PFVLPLH+SFGIEN+ SFE V+SFV L+ 
Sbjct: 66  KGLEELFDLPLETKMKNVSDKPYHGFLDHRTPFVLPLHQSFGIENSESFEAVQSFVNLMW 125

Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
                   +++F+YTKMVSEMEKLVK MVFESYGV KYYDSHIASTTYLLR MKYRIPEM
Sbjct: 126 PSGNRRFCENVFSYTKMVSEMEKLVKVMVFESYGVKKYYDSHIASTTYLLRTMKYRIPEM 185

Query: 185 GEQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNG 244
           GE+NIGA+AHTDKSFFTILHQN                                 AWSNG
Sbjct: 186 GEENIGADAHTDKSFFTILHQNEVNGLEIKTKDGHWLGVEFTPNSFLVMAGDACLAWSNG 245

Query: 245 RIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKE 279
           RIHSATHRV+IEG+KERYSTALFSYHKG+IEIP ELVDE+ PL+F+PFNHYGLL YFS  
Sbjct: 246 RIHSATHRVMIEGTKERYSTALFSYHKGIIEIPYELVDEKHPLRFHPFNHYGLLAYFSTH 305

[Close]

BLAST of Carg04251-RA vs. NCBI nr
Match: XP_016900413.1 (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo])

HSP 1 Score: 332.0 bits (850), Expect = 1.9e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0


Query: 79  MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
           MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+         ++LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
           +K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
           FFTILHQ    NG                              AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
           G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

[Close]

BLAST of Carg04251-RA vs. TAIR10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 236.9 bits (603), Expect = 1.5e-62
Identity = 125/311 (40.19%), Postives = 180/311 (57.88%), Query Frame = 0


Query: 9   PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
           P++P ++FS E+L+P +  W  T  ++R ALEEYGCF+ + ++  +  ++L D +F  L 
Sbjct: 3   PKIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD--KTPLDLLDRVFGSLV 62

Query: 69  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
            LFDLP QTKMKN  DKP +G++  + P  LPLHES GI+NA S E   SF  L+     
Sbjct: 63  DLFDLPTQTKMKNKYDKPLNGYVG-QIP-ALPLHESLGIDNATSLEATRSFTGLMWPQGN 122

Query: 129 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 188
               + L+ Y +  +E++++V RMVF+SY V KYYD +I STTYLLR +K R P      
Sbjct: 123 EHFSECLYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPT 182

Query: 189 IGAEAHTDKSFFTILHQ---NG------------------------------AWSNGRIH 248
           +G   HTDKSF TILHQ   NG                              AWSN R+ 
Sbjct: 183 LGFVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVW 242

Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 278
           S  H+V++ G  +RYS  +F+++ G +++P+EL+D Q PL + PF+H GLL ++  +   
Sbjct: 243 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 302

[Close]

BLAST of Carg04251-RA vs. TAIR10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 221.5 bits (563), Expect = 6.6e-58
Identity = 116/311 (37.30%), Postives = 173/311 (55.63%), Query Frame = 0


Query: 10  EVPVINF-SIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 69
           +VP+++  S + L+P ++ W         ALEEYGCFLA Y+   +Q   LDD+IF   E
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQ---LDDSIFAAAE 68

Query: 70  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 129
           +LFDLP +TK KNV++KPYHG++      V+PLHE  G++   + E  + F  L+     
Sbjct: 69  ELFDLPTETKKKNVNEKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGN 128

Query: 130 ----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQN 189
                 +  ++  V+E+++LV RM+FE+YGV K+Y+SH+ S TYLL+ +KY  P      
Sbjct: 129 DRFCNTVHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISM 188

Query: 190 IGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIHS 249
                HTDK+F +ILHQN                                  WSN RI S
Sbjct: 189 PAFPQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPKSYVVMAGDISMGWSNDRIRS 248

Query: 250 ATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEK 279
             HRV +EG K RY+  LFS+   ++ IP+ELVD++ PL + PF++  L+ +++ +E  +
Sbjct: 249 CEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGRE 308

[Close]

BLAST of Carg04251-RA vs. TAIR10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 212.6 bits (540), Expect = 3.1e-55
Identity = 119/322 (36.96%), Postives = 180/322 (55.90%), Query Frame = 0


Query: 1   MGFRSSTKP-EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVEL 60
           MG  +   P  +PVI+FS +NL+P   +W  T   V+ AL++YG F A ++       EL
Sbjct: 1   MGSETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFD---RIPFEL 60

Query: 61  DDAIFNGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESF 120
             ++F  LE+LFDLPLQTK++NVS KP+HG++      ++PL+ES GI+++   E V++F
Sbjct: 61  RKSVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAF 120

Query: 121 ---------VKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY 180
                    +     + +++K +SE++  ++RM+ ES+G+ KY D H+ ST YLLR MKY
Sbjct: 121 TEKLWPQGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKY 180

Query: 181 RIPEMGEQNIGAEAHTDKSFFTILHQNG-------------------------------- 240
           + P+  E  +G  AHTDK+  TIL+QN                                 
Sbjct: 181 KGPDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLY 240

Query: 241 AWSNGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGL 279
           A  NGR+HS  HRV++ G++ RYS  LFS  K   ++  PDELVDE+ P  F PF+H   
Sbjct: 241 ALLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEF 300

[Close]

BLAST of Carg04251-RA vs. TAIR10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 113/316 (35.76%), Postives = 163/316 (51.58%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           E+P+IN S +NL+  +  W  T   VR A+E +G F+AEY N       L  +I    ++
Sbjct: 8   ELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNN---FPTGLHQSILEAAKE 67

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI------ 129
           L DLP++ K+KN + K  HG++   +    P+HE  GI+     +    F +L+      
Sbjct: 68  LLDLPVEIKLKNENHKAGHGYITMMSD-GQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHD 127

Query: 130 ------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGE 189
                 + + AY KM +E+E+LV RM+FESY V KY + +I  T YLLR +KYR    GE
Sbjct: 128 DNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGE 187

Query: 190 QNIGAEAHTDKSFFTILHQNG---------------------------------AWSNGR 249
            N    +HTDKSF +ILHQN                                  AWSN R
Sbjct: 188 PNRKFISHTDKSFISILHQNHITGLMLKSEKEDVWYHFTPSPTRFVVIAGDAIMAWSNDR 247

Query: 250 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           I +  H+V +E  + RYS   FS+ +G+I  P+E+VD+  PL +NPF+H GLL Y+   E
Sbjct: 248 IKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLE 307

[Close]

BLAST of Carg04251-RA vs. TAIR10
Match: AT4G03070.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 189.9 bits (481), Expect = 2.1e-48
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
                 +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

[Close]

BLAST of Carg04251-RA vs. Swiss-Prot
Match: sp|Q9ZTA3|AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 3.8e-47
Identity = 111/304 (36.51%), Postives = 161/304 (52.96%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI +A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------RIP 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 EMGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------S 249
                 +G  +HTDK+  TILHQ           +  W                      
Sbjct: 193 XXXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALL 252

Query: 250 NGRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGY 264
           NGR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

[Close]

BLAST of Carg04251-RA vs. Swiss-Prot
Match: sp|Q945B6|AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 6.5e-47
Identity = 111/303 (36.63%), Postives = 162/303 (53.47%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFD---KLSVELNRSVFEAMED 72

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS KP+HG+L H       L+ES GI++A   E V  F +        
Sbjct: 73  LFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWPDHGN 132

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY-----RIPE 189
             + + +  +++ + E++ +V+RM+ ES+G+  Y D H+ ST YL R MKY         
Sbjct: 133 KSISETIHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYXXXXXXXXX 192

Query: 190 MGEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SN 249
                +G  +HTDK+  TILHQ           +  W                      N
Sbjct: 193 XXXXKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLN 252

Query: 250 GRIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYF 264
           GR+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F
Sbjct: 253 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 306

[Close]

BLAST of Carg04251-RA vs. Swiss-Prot
Match: sp|Q944Z5|AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 8.5e-47
Identity = 111/302 (36.75%), Postives = 161/302 (53.31%), Query Frame = 0


Query: 10  EVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLEK 69
           ++PVI+FS +NL+P S+KW E    V  ALE+YGCF A ++   +  VEL+ ++F  +E 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFD---KLSVELNRSVFEAMED 65

Query: 70  LFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVK-------- 129
           LF+LP+ TK +NVS K +HG+L H       L+ES GI +A   E V  F +        
Sbjct: 66  LFELPIPTKQRNVSSKLFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWPDHGN 125

Query: 130 --LIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY----RIPEM 189
             + + +  +++ + E++ +V+RM+ ES+G+ KY D H+ ST YL R MKY         
Sbjct: 126 KSISETIHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTXXXXXXXX 185

Query: 190 GEQNIGAEAHTDKSFFTILHQ-----------NGAW---------------------SNG 249
               +G  +HTDK+  TILHQ           +  W                      NG
Sbjct: 186 XXTKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDSVLVMVGDSLCALLNG 245

Query: 250 RIHSATHRVIIEGSKERYSTALFSYHKG--VIEIPDELVDEQFPLKFNPFNHYGLLGYFS 264
           R+HS  HRVI+ G K RYST LFS  K   +I+ P+ELVD++ P  F PF +   L +F 
Sbjct: 246 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 298

[Close]

BLAST of Carg04251-RA vs. Swiss-Prot
Match: sp|Q945B4|AOP3L_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.7e-34
Identity = 114/412 (27.67%), Postives = 169/412 (41.02%), Query Frame = 0


Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P++P+I  S + L+P S+KWV+  + VR ALE+YGCF A+ +   +  +EL  ++  
Sbjct: 3   SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFV----- 125
            +++LF LP + K +NV  KP+ G+  H       L ESFGI++A   E    F      
Sbjct: 63  AMQELFALPTEAKQRNVCPKPFAGYFSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122

Query: 126 ---KLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY------- 185
              K I+ +  Y + ++E++ +V+R++ ESYG+  + D H+ ST Y +R MKY       
Sbjct: 123 EGNKSIKMIQLYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIARPDND 182

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 183 ITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 246 -----------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN---- 279
                                      +G  E N+ AE    +HTDKS   I++Q+    
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLSGIIYQHQIDG 302

[Close]

BLAST of Carg04251-RA vs. Swiss-Prot
Match: sp|Q9ZTA1|AOP3C_ARATH (2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP3 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 4.1e-33
Identity = 118/416 (28.37%), Postives = 171/416 (41.11%), Query Frame = 0


Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P++P+I  S + L+P S+KWV+  + VR ALE+YGCF A+ +   +  +EL  ++  
Sbjct: 3   SCSPQLPLICLSDQTLKPGSSKWVKVRSDVRKALEDYGCFEAKID---QVSMELQGSVLK 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGI------ENAASF------ 125
            +++LF LP + K +NV  KP+ G+L H       L ESFGI      E A  F      
Sbjct: 63  AMQELFALPTEAKQRNVCPKPFTGYLSHNG-----LSESFGIKDANILEKAHEFTQQLWP 122

Query: 126 EGVESFVKLIQDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKY--- 185
           EG +S  K+IQ    Y + ++E++ +V+R++ ESYG+  + D H+ ST Y +R MKY   
Sbjct: 123 EGNKSISKMIQ---LYAEKLAELDMMVRRLILESYGIEYFIDEHLNSTYYRMRLMKYIAR 182

Query: 186 ------------------------------------------------------------ 245
                                                                       
Sbjct: 183 PDNDITAAVGANVDNGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 242

Query: 246 ---------------------------RIPEMG--EQNIGAE----AHTDKSFFTILHQN 279
                                          +G  E N+ AE    +HTDKS   I++Q+
Sbjct: 243 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVGAKEANVDAELGLPSHTDKSLTGIIYQH 302

[Close]

BLAST of Carg04251-RA vs. TrEMBL
Match: tr|A0A1S4DWR1|A0A1S4DWR1_CUCME (2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103490084 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.3e-87
Identity = 166/243 (68.31%), Postives = 187/243 (76.95%), Query Frame = 0


Query: 79  MKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI---------QDLFAY 138
           MKNVSDKPYHGFLD RAPFVLPLH+S GIENA+S+E V+SFV L+         ++LFAY
Sbjct: 1   MKNVSDKPYHGFLDSRAPFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHFCENLFAY 60

Query: 139 TKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQNIGAEAHTDKS 198
           +K ++EME+LVKRMVFESYGVGKY DSHIASTTYLLR MKYRIP+MGE+NIGAEAHTDKS
Sbjct: 61  SKKITEMEQLVKRMVFESYGVGKYTDSHIASTTYLLRTMKYRIPKMGEENIGAEAHTDKS 120

Query: 199 FFTILHQ----NG------------------------------AWSNGRIHSATHRVIIE 258
           FFTILHQ    NG                              AWSNGRIHSATHRVIIE
Sbjct: 121 FFTILHQFDGVNGLQIKPKNYDQWLGVHFSPNYFLVMAGDACLAWSNGRIHSATHRVIIE 180

Query: 259 GSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECEKTPSTAGAY 279
           G+KERYSTALFSYHKG+IEIP ELVD++ PL+F PFNHYGLLGYFS ++C+K PSTA AY
Sbjct: 181 GNKERYSTALFSYHKGIIEIPQELVDDKHPLRFKPFNHYGLLGYFSTDDCDKMPSTANAY 240

[Close]

BLAST of Carg04251-RA vs. TrEMBL
Match: tr|W9STP2|W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.8e-79
Identity = 156/312 (50.00%), Postives = 200/312 (64.10%), Query Frame = 0


Query: 9   PEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFNGLE 68
           P++P I+FS  NL P ++ W   C  VR ALEEYGCF+A Y+   +Q   L  ++F+ L+
Sbjct: 7   PKIPTIDFSEGNLNPGTSSWFSVCNDVRRALEEYGCFVALYDKISQQ---LRTSMFSELK 66

Query: 69  KLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI----- 128
           +LFDLP + KMKNVSDKPY G++ +  P + PLHES GIENA + EG ESF  +I     
Sbjct: 67  ELFDLPTEIKMKNVSDKPYFGYIGNH-PSIPPLHESMGIENATTLEGGESFTNIIWPKGG 126

Query: 129 -----QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMGEQ 188
                + + +Y+++VSE+EK+VKRMVF+SYGV KYYDSH+ STT+LL+ +KYR+PEM E 
Sbjct: 127 NNHFCETVVSYSRLVSELEKMVKRMVFQSYGVEKYYDSHLGSTTFLLKLIKYRVPEMNES 186

Query: 189 NIGAEAHTDKSFFTILHQNG--------------------------------AWSNGRIH 248
           +IG   HTDKSF T+LHQN                                 AW+NGRIH
Sbjct: 187 SIGCAPHTDKSFITVLHQNEVNGLEIETRDGDWIQFEPSPSSFLVLAGDAFLAWTNGRIH 246

Query: 249 SATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEECE 279
           SA HRV++ G+K RYS  LFSYH G IEIPDELVDEQ P +F  F+HYGLL ++      
Sbjct: 247 SAAHRVVMTGTKPRYSIGLFSYHNGTIEIPDELVDEQHPQQFKSFDHYGLLHHY--HAAP 306

[Close]

BLAST of Carg04251-RA vs. TrEMBL
Match: tr|A5AJI0|A5AJI0_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.4e-78
Identity = 154/314 (49.04%), Postives = 199/314 (63.38%), Query Frame = 0


Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P +PV+NFS   L+P +  W+  C  V  ALEEYGCF+A Y+   +   ELD+  F 
Sbjct: 3   SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
            LE+LFDLP +TK+KN SDKPY G++      ++PLHES GIE+A + +GVESF  ++  
Sbjct: 63  QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122

Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
                  + + +Y+K+VSE+E++VK+MVFESY   KYYDSHI STTYLLR +KYR P+  
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182

Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
           E  IG   HTDKSF T+LHQN                                 AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242

Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           IHS  HRVI+ G+K RYS  LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++   +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302

[Close]

BLAST of Carg04251-RA vs. TrEMBL
Match: tr|D7U0Q7|D7U0Q7_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.3e-78
Identity = 153/314 (48.73%), Postives = 198/314 (63.06%), Query Frame = 0


Query: 6   STKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIFN 65
           S  P +PV+NFS   L+P +  W+  C  V  ALEEYGCF+A Y+   +   ELD+  F 
Sbjct: 3   SEAPRLPVLNFSTGTLKPGTRSWLLACKDVLHALEEYGCFVAVYD---KVSSELDNTTFC 62

Query: 66  GLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI-- 125
            LE+LFDLP +TK+KN SDKPY G++      ++PLHES GIE+A + +GVESF  ++  
Sbjct: 63  QLEELFDLPTETKVKNTSDKPYFGYIGQHP--LIPLHESMGIEDATTLKGVESFTNIMWP 122

Query: 126 -------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEMG 185
                  + + +Y+K+VSE+E++VK+MVFESY   KYYDSHI STTYLLR +KYR P+  
Sbjct: 123 AGNDHFCETVLSYSKLVSELEQMVKKMVFESYDAEKYYDSHIQSTTYLLRLIKYRGPQKN 182

Query: 186 EQNIGAEAHTDKSFFTILHQNG--------------------------------AWSNGR 245
           E  IG   HTDKSF T+LHQN                                 AWSNGR
Sbjct: 183 ETCIGTSCHTDKSFITVLHQNEVCGLEIRTKDGEWISFKPSPSSYVVMAGDALLAWSNGR 242

Query: 246 IHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSKEE 279
           IHS  HRVI+ G+K RYS  LF+YH+G+IEIP+EL DE+ PL+F PFNHYGLL ++   +
Sbjct: 243 IHSPEHRVIMNGNKARYSIGLFAYHRGIIEIPEELADEEHPLQFKPFNHYGLLHFYFTNK 302

[Close]

BLAST of Carg04251-RA vs. TrEMBL
Match: tr|B9RUX7|B9RUX7_RICCO (Gibberellin 20 oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_0896520 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.0e-73
Identity = 146/316 (46.20%), Postives = 195/316 (61.71%), Query Frame = 0


Query: 5   SSTKPEVPVINFSIENLRPKSTKWVETCTHVRAALEEYGCFLAEYENDVEQEVELDDAIF 64
           S   P++PVI+FS ENL+P ++ WV TC +V  ALEEYGCF+A YE   +  +EL  + +
Sbjct: 3   SRIPPKIPVIDFSQENLKPGTSSWVSTCKNVLRALEEYGCFIAVYE---KIPLELQSSFY 62

Query: 65  NGLEKLFDLPLQTKMKNVSDKPYHGFLDHRAPFVLPLHESFGIENAASFEGVESFVKLI- 124
           N L  LFDLP+ TK KNVS  PY+G++ ++ PFV PL+E  GI NAA+ EG   F  ++ 
Sbjct: 63  NKLITLFDLPIDTKRKNVSAIPYYGYVGNQ-PFVPPLYEGMGINNAATMEGTLDFTNIMW 122

Query: 125 --------QDLFAYTKMVSEMEKLVKRMVFESYGVGKYYDSHIASTTYLLRGMKYRIPEM 184
                   + +F+Y+K V+E+E++V RMVFE YGV   YD+    T YL R MKYR P  
Sbjct: 123 PNGDDDFCKTVFSYSKWVAELEQMVTRMVFEGYGVQYSYDTQQERTNYLFRMMKYRAPTE 182

Query: 185 GEQNIGAEAHTDKSFFTILHQNG---------------------------------AWSN 244
           GE ++G + HTDK F T+LHQN                                  AWSN
Sbjct: 183 GESDLGCDVHTDKGFITVLHQNEVSGLEVQTKDGQWVCFEPSSPSSFIVMAADALLAWSN 242

Query: 245 GRIHSATHRVIIEGSKERYSTALFSYHKGVIEIPDELVDEQFPLKFNPFNHYGLLGYFSK 279
           GRIH+A HRV++ GSK RYS  LFSY KG+ E+P+EL+DE+ PL+F PF+++GLL YF  
Sbjct: 243 GRIHAAFHRVVMRGSKARYSVGLFSYLKGITEVPEELIDEEHPLQFKPFDNFGLLHYFYT 302

The following BLAST results are available for this feature:

BLAST of Carg04251-RA vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022948698.1	6.4e-152	85.94	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita moschata]	[more]
XP_023525673.1	1.8e-149	84.06	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997882.1	1.0e-144	82.50	2-oxoglutarate-dependent dioxygenase AOP3-like [Cucurbita maxima]	[more]
XP_022153580.1	1.4e-114	67.30	2-oxoglutarate-dependent dioxygenase AOP3-like [Momordica charantia]	[more]
XP_016900413.1	1.9e-87	68.31	PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP3-like [Cucumis melo]	[more]

BLAST of Carg04251-RA vs. TAIR10


Match Name	E-value	Identity	Description
AT1G52790.1	1.5e-62	40.19	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	6.6e-58	37.30	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	3.1e-55	36.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	2.1e-48	35.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G03070.1	2.1e-48	36.51	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

BLAST of Carg04251-RA vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q9ZTA3\|AOP1C_ARATH	3.8e-47	36.51	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
sp\|Q945B6\|AOP1L_ARATH	6.5e-47	36.63	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
sp\|Q944Z5\|AOP1V_ARATH	8.5e-47	36.75	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
sp\|Q945B4\|AOP3L_ARATH	3.7e-34	27.67	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]
sp\|Q9ZTA1\|AOP3C_ARATH	4.1e-33	28.37	2-oxoglutarate-dependent dioxygenase AOP3 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of Carg04251-RA vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S4DWR1\|A0A1S4DWR1_CUCME	1.3e-87	68.31	2-oxoglutarate-dependent dioxygenase AOP3-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
tr\|W9STP2\|W9STP2_9ROSA	4.8e-79	50.00	Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis OX=981085 GN=L484_019467 PE=...	[more]
tr\|A5AJI0\|A5AJI0_VITVI	1.4e-78	49.04	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_028513 PE=3 SV=1	[more]
tr\|D7U0Q7\|D7U0Q7_VITVI	5.3e-78	48.73	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_09s0002g05290 PE=3 SV=...	[more]
tr\|B9RUX7\|B9RUX7_RICCO	3.0e-73	46.20	Gibberellin 20 oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_0896520 PE=...	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg04251	Carg04251	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg04251-RA	Carg04251-RA-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04251-RA.exon.1	Carg04251-RA.exon.1	exon
Carg04251-RA.exon.2	Carg04251-RA.exon.2	exon
Carg04251-RA.exon.3	Carg04251-RA.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04251-RA.CDS.1	Carg04251-RA.CDS.1	CDS
Carg04251-RA.CDS.2	Carg04251-RA.CDS.2	CDS
Carg04251-RA.CDS.3	Carg04251-RA.CDS.3	CDS

InterPro

Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 199..263 e-value: 7.5E-10 score: 40.5
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 2..198 e-value: 2.7E-18 score: 68.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 11..90 e-value: 8.8E-7 score: 29.6
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 7..197 coord: 199..277
None	No IPR available	PANTHER	PTHR10209:SF182	2-OXOGLUTARATE 2OG AND FE II -DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 7..197 coord: 199..277
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 8..261