CmaCh17G008320 (gene) Cucurbita maxima (Rimu)

NameCmaCh17G008320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCma_Chr17 : 6529823 .. 6531815 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCTTTTGTCTCTCTTACATAAGCTATCAAACCAAGGAAGAACAGGTAGCAGTGAGTTAGGCATTGGTGAGAATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGACGTTGGAGGCTGGCACTGAAGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTTTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGTCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGAAAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCTGCTCAAGGAAACAATCACTTCAGGTAATTATTAGAATATTATGTTACGAGGTGTAGATTTTAACTTCTGAGCTCGAATTGAACGTCTTTTAACCATATTCATGTTTGTGAAAGTTACCTAAGAAAATTGACCCACCGGAGAAAAAGTTAAGTTATAAGTTTAGTTTATGAACTTTGATGGTGGTAAACTTTTAATTTTTTGTTTAATAGATTTGTAAATTTTTAGAAATTTTGATTAAGTGAGACATGGTGTCTAACACTTTACCACTCTGTGGTCTAAGGGAGCTATCTTATGCTACGTGAGTGTAATGGTTGGACGAACGTTGTAAGATGAATGTCTTGGGCGTCTTCCTCGTTTTTGAAAATAATGTTATAGGCATGGGTGTGTTGAGATTGTGTCATGTCAAACTTATTAAATATTTAAACTTTAGGATCAAATCGACACAAACTTGAAAGCTCATAAATTGTGAGATCCCACGTCAGTATTCCTTGTAAGGGTATGGGAAACCTTTCTCTAGGAGACCCATTTTTAAACCTTTCTCTAGGAGACCCATTTTTAAACCTTTCTCTAGGAGACCCATTTTTAAAACCATGAGGTTTTCTTGTAAGGGTATGGAAAACCTTTCACAAATGGGGTGAATTGTGAGATCTCACTTGGAATACGGATGGAGTGAAAATGAATTATTGGAAGTTTTTCATTTTTATGTAATGACAGCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAATAACAAGAATGGTGTTGGAAAGCTATGGAGTAACAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACCGAGTTCCTGAGGAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCCCTCGACGATCAATGGATCGACCTCGAGCCCTCCGGCCACTGCTCCTTCGTCGTCATGGCGGGTGACGCGCTAATGGTATCCACATTTCATCATTCTAGTTCATATCAATTATCGTTGAACTATACTCACTTTATCTGAATGAATGAACGTAGGCATGGAGCAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACAATGAAAGCGGAGGAAACGAGATACTCAGCTGGGATGTTTTCGTTCAAGAATGGGACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTTTGACTTCCTTACTTATGACAAAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGAATCAACAAAACTTGAAACTCTGTCCAATATATATGAATTATTGTTCATCAATAAGTTTGCCTGTAAAATATTTCCCTTTCTTCCTTCTATAATCCGAACATTTGTACCGATTAATGTTATTGTGCTTGTTTTCTCACCGTTTATTTACAAAAGTTTATGAAAAAAAAGTTTGAATATTATGTTTCCCGAATGATGCTACT

mRNA sequence

CAACCTTTTGTCTCTCTTACATAAGCTATCAAACCAAGGAAGAACAGGTAGCAGTGAGTTAGGCATTGGTGAGAATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGACGTTGGAGGCTGGCACTGAAGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTTTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGTCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGAAAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCTGCTCAAGGAAACAATCACTTCAGCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAATAACAAGAATGGTGTTGGAAAGCTATGGAGTAACAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACCGAGTTCCTGAGGAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCCCTCGACGATCAATGGATCGACCTCGAGCCCTCCGGCCACTGCTCCTTCGTCGTCATGGCGGGTGACGCGCTAATGGCATGGAGCAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACAATGAAAGCGGAGGAAACGAGATACTCAGCTGGGATGTTTTCGTTCAAGAATGGGACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTTTGACTTCCTTACTTATGACAAAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGAATCAACAAAACTTGAAACTCTGTCCAATATATATGAATTATTGTTCATCAATAAGTTTGCCTGTAAAATATTTCCCTTTCTTCCTTCTATAATCCGAACATTTGTACCGATTAATGTTATTGTGCTTGTTTTCTCACCGTTTATTTACAAAAGTTTATGAAAAAAAAGTTTGAATATTATGTTTCCCGAATGATGCTACT

Coding sequence (CDS)

ATGGGGTCCGAAACACAAGCCAGAGTGCCCGTAATAGACTTGTCCAAATTAGAGACGTTGGAGGCTGGCACTGAAGCATGGGGCTCAACATGCAAGGCGATGAGGGTTGGTTTGGAGGAATTTGGGTGCTTTTGTGTGGTTTTTGAGGAGGTTTCTTTGGAGCTTCATAACTCCTTCTTTGGAGCTGTTGATGAGCTGTTTCAGCTTCCAATTGAAACTCGAATCAAGAACACAAGTGAAAAGGCTTACCATGGCTACTTTGGTCGCTACTCCTTTCTTCCTCTCTATGAATCCATGGCTATTGAAAACCCAACTCAGCTAGACGCTGCTCAAAGCTTTGCCAATCTCATGTGGCCTGCTCAAGGAAACAATCACTTCAGCGAAACTATTCAACGTTTCTCAACCTTGATGGCAAAACTGGACAAGATAATAACAAGAATGGTGTTGGAAAGCTATGGAGTAACAAAGCAGCTGATCGACACAAACATAGATTCAACAGCCAACCTTCTCCGCTTCTTCAAATACCGAGTTCCTGAGGAACACGAAACCGATGTGGGACTGCACGCCCATACAGATCTCACTTTTTTCTCCATAATTCATCAACATCTCATCGGTGGTCTCCAAATTCAATCCCTCGACGATCAATGGATCGACCTCGAGCCCTCCGGCCACTGCTCCTTCGTCGTCATGGCGGGTGACGCGCTAATGGCATGGAGCAACGATAGAATAAGGAGTTGTAGGCATCGAGTGACAATGAAAGCGGAGGAAACGAGATACTCAGCTGGGATGTTTTCGTTCAAGAATGGGACGATTAGTGTGCCTGAAGAGCTTGTGGATGATGCCAACCCATTGCGATACAAGCCCTTTAATCACTTTGACTTCCTTACTTATGACAAAGCAAAGGGTTCTAAAAAATTTCTCTATCGTATTAGAGATTATTGCAGCATTTGA

Protein sequence

MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAKGSKKFLYRIRDYCSI
BLAST of CmaCh17G008320 vs. Swiss-Prot
Match: AOP1V_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.9e-59
Identity = 121/303 (39.93%), Postives = 184/303 (60.73%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ G+  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 6   QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 65

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFS 127
           +LPI T+ +N S K +HGY        LYES+ I +   L+    F   +WP  GN   S
Sbjct: 66  ELPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 125

Query: 128 ETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVP----EEHET 187
           ETI  FS  + +LD ++ RM++ES+G+ K  ID +++ST  L R  KY  P    ++ ET
Sbjct: 126 ETIHLFSEQLVELDLMVRRMIMESFGIEK-YIDEHLNSTYYLTRLMKYTSPPDDDDDEET 185

Query: 188 DVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDRI 247
            +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N R+
Sbjct: 186 KLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGRL 245

Query: 248 RSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHFDFLTYDKAK 305
            S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL + + +
Sbjct: 246 HSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTE 301

BLAST of CmaCh17G008320 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.1e-58
Identity = 120/304 (39.47%), Postives = 185/304 (60.86%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ G+  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFS 127
           +LPI T+ +N S K +HGY        LYES+ I++   L+    F   +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVP-----EEHE 187
           ETI  FS  + +LD ++ RM++ES+G+ +  ID +++ST  L R  KY  P     ++ E
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEE 192

Query: 188 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDR 247
           T +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N R
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNGR 252

Query: 248 IRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHFDFLTYDKA 305
           + S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL + + 
Sbjct: 253 LHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQT 309

BLAST of CmaCh17G008320 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 120/305 (39.34%), Postives = 184/305 (60.33%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ G+  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFS 127
           +LPI T+ +N S K +HGY        LYES+ I +   L+    F   +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVP------EEH 187
           ETI  FS  + +LD ++ RM++ES+G+ +  ID +++ST  L R  KY  P      ++ 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 188 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSND 247
           ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N 
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNG 252

Query: 248 RIRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHFDFLTYDK 305
           R+ S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL + +
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 310

BLAST of CmaCh17G008320 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.4e-28
Identity = 71/194 (36.60%), Postives = 110/194 (56.70%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS    ++P+I+L+  +TLE G+  W      +R  LE+FGCF   +++VSLEL  S  
Sbjct: 1   MGS-CSLQLPLINLAD-KTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIM 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWP- 120
             ++ELF LP+ET+ +N   K Y GY    +   L ES+ I N   L+    F   +WP 
Sbjct: 61  KTMEELFALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPD 120

Query: 121 AQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPE 180
             GN + S+TIQ F+  + ++D ++ RMV+ES+G+ K  ID ++ ST   +R  KY  P 
Sbjct: 121 GDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEK-YIDDHLKSTEYRMRLMKYIAPP 180

Query: 181 EHETDVGLHAHTDL 194
           E + +  +  + DL
Sbjct: 181 EGDANTTVDDYADL 188

BLAST of CmaCh17G008320 vs. Swiss-Prot
Match: AOP2L_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 9.9e-28
Identity = 70/194 (36.08%), Postives = 110/194 (56.70%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS    ++P+I+L+  +TLE G+  W      +R  L++FGCF   +++VSLEL  S  
Sbjct: 1   MGS-CSLQLPLINLAD-KTLEPGSSKWAEVRSDVRKALQDFGCFEASYDKVSLELQESIM 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWP- 120
             ++ELF LP+ET+ +N   K Y GY    +   L ES+ I N   L+    F   +WP 
Sbjct: 61  KTMEELFALPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENINEFTQQLWPH 120

Query: 121 AQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPE 180
             GN + S+TIQ F+  + ++D ++ RMV+ES+G+ K  ID ++ ST   +R  KY  P 
Sbjct: 121 GDGNENISKTIQLFAEKLVEIDVMVRRMVMESFGIEK-YIDDHLKSTEYRMRLMKYIAPP 180

Query: 181 EHETDVGLHAHTDL 194
           E + +  +  + DL
Sbjct: 181 EGDANTTVDDYADL 188

BLAST of CmaCh17G008320 vs. TrEMBL
Match: M5WSX6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008973mg PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.4e-99
Identity = 178/316 (56.33%), Postives = 234/316 (74.05%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQ ++PVIDLS  E L+ GTEAW    K ++  LEE+GCF  VF +V L+LHNS F
Sbjct: 1   MGSETQPKLPVIDLSN-ENLKPGTEAWVLASKHVKYALEEYGCFEAVFHKVPLQLHNSIF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
             V++LF LPIET+ + TSE+ YH YFG+YSFLPLYES+ ++NPT ++ A+ F  +MWPA
Sbjct: 61  SVVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAKGFTKIMWPA 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN+HF E+ Q FS L+A+LD+++TRMV + YGV ++L D+++ ST  LLR FKYR  +E
Sbjct: 121 -GNDHFCESAQSFSKLVAELDQMVTRMVFDIYGV-ERLYDSHMASTTYLLRCFKYREQKE 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +ET+VGL  H D TF SI+HQ  + GLQ++S D QWI+ EPS   SF+VMAGDA MAWSN
Sbjct: 181 NETNVGLRPHKDKTFTSILHQSQVHGLQVRSKDGQWIEAEPS-PSSFLVMAGDAFMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
           DR+RSC H+V MK  +TRYS G+FSF +G + VPEEL DD +PL YKPF+HF FL +D+ 
Sbjct: 241 DRVRSCDHQVIMKENKTRYSVGLFSFNSGILQVPEELTDDQHPLLYKPFDHFSFLRFDQT 300

Query: 301 KGSKKFLYRIRDYCSI 317
             +KK    I+ YC +
Sbjct: 301 PEAKKSECPIKAYCGV 312

BLAST of CmaCh17G008320 vs. TrEMBL
Match: V4ST91_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027420mg PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-90
Identity = 168/318 (52.83%), Postives = 228/318 (71.70%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS+T++++PVIDLSK + L++GT  W STCK +R   EEFGCF  +++++S E  +   
Sbjct: 1   MGSQTKSKIPVIDLSK-QDLKSGTSTWLSTCKDVRHAFEEFGCFEAIYQKISQEFRSEVL 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
            + +ELF +PIE ++KNTS K Y  Y+G+Y+ +PLYES+AI+ P   +A QSF NLMWPA
Sbjct: 61  ASTEELFDVPIEIKVKNTSTKPYFEYYGQYTIIPLYESLAIDYPDTRNATQSFTNLMWPA 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN+ F E+    S L+ +LDK++TRM+ ESYGV +   D+ I S   LLR+FKYR PE 
Sbjct: 121 -GNDCFCESSLSMSELVVELDKMVTRMLFESYGV-EGYYDSYIGSVNYLLRYFKYRAPEP 180

Query: 181 HETDVGLHAHTDLTFFSIIHQ-HLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWS 240
           +ET +GL  HTD T  SIIHQ + I GLQ+Q+ D +WID+EPS   SF+VMAGDALMAW 
Sbjct: 181 NETKMGLTPHTDKTMTSIIHQINHINGLQVQAKDGEWIDVEPS-PSSFIVMAGDALMAWG 240

Query: 241 NDRIRSCRHRVTM-KAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYD 300
           NDRIR CRH+V M  A ETRYS G+FSF +G + +P+EL D+  PL+YKPF+HF FL ++
Sbjct: 241 NDRIRPCRHQVIMDNASETRYSLGLFSFSSGVVDIPKELGDETQPLKYKPFDHFGFLHFN 300

Query: 301 KAKGSKKFLYRIRDYCSI 317
           +++  KK    I+ YC I
Sbjct: 301 QSEEGKKSASSIKAYCGI 314

BLAST of CmaCh17G008320 vs. TrEMBL
Match: V4SN85_9ROSI (Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10027182mg PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.9e-90
Identity = 161/316 (50.95%), Postives = 225/316 (71.20%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS+TQ ++ V+DL   E L+ GT  W STC  +R  +E+ GCF  ++ ++ LELHN  F
Sbjct: 12  MGSQTQPKILVVDLCN-ENLKPGTSTWISTCNEIRQAMEDTGCFEAIYNKIPLELHNEIF 71

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
            A DELF LPIET+++NTS+K YH YFG+Y+ +PLYES+AI++PT L A QSF + MWP 
Sbjct: 72  KAADELFDLPIETKMQNTSDKPYHEYFGQYTGIPLYESLAIDDPTNLTATQSFTDRMWP- 131

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN  F E++  F+ ++ ++D+++ RM++ESYG+ K   D++I+++  LLRFFKYR PE 
Sbjct: 132 NGNPPFCESVHSFAKIIVEVDQLVMRMIMESYGIEK-YYDSHIETSNYLLRFFKYRKPEI 191

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +E +VGL+ HTD    SIIHQ+ I GLQI++ D +WIDLEPS   SFV+MA D L+AWSN
Sbjct: 192 NEHNVGLYPHTDKNMVSIIHQNHINGLQIKAKDGEWIDLEPS-PSSFVIMAADGLLAWSN 251

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
            RIRSC H+V + A ETRYS G+FSF  G + +PEE VD+ +PLRY+PF+ ++FL +  +
Sbjct: 252 GRIRSCEHQVIINAHETRYSMGLFSFNRGIVHIPEEFVDELHPLRYRPFDIYEFLRFHDS 311

Query: 301 KGSKKFLYRIRDYCSI 317
              KK    I  +C I
Sbjct: 312 DEGKKTDGSITSFCGI 323

BLAST of CmaCh17G008320 vs. TrEMBL
Match: A0A067F428_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037660mg PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 2.9e-90
Identity = 161/316 (50.95%), Postives = 225/316 (71.20%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS+TQ ++ V+DL   E L+ GT  W STC  +R  +E+ GCF  ++ ++ LELHN  F
Sbjct: 1   MGSQTQPKILVVDLCN-ENLKPGTSTWISTCNEIRQAMEDTGCFEAIYNKIPLELHNEIF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
            A DELF LPIET+++NTS+K YH YFG+Y+ +PLYES+AI++PT L A QSF + MWP 
Sbjct: 61  KAADELFDLPIETKMQNTSDKPYHEYFGQYTGIPLYESLAIDDPTNLTATQSFTDRMWP- 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN  F E++  F+ ++ ++D+++ RM++ESYG+ K   D++I+++  LLRFFKYR PE 
Sbjct: 121 NGNPPFCESVHSFAKIIVEVDQLVMRMIMESYGIEK-YYDSHIETSNYLLRFFKYRKPEI 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +E +VGL+ HTD    SIIHQ+ I GLQI++ D +WIDLEPS   SFV+MA D L+AWSN
Sbjct: 181 NEHNVGLYPHTDKNMVSIIHQNHINGLQIKAKDGEWIDLEPS-PSSFVIMAADGLLAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
            RIRSC H+V + A ETRYS G+FSF  G + +PEE VD+ +PLRY+PF+ ++FL +  +
Sbjct: 241 GRIRSCEHQVIINAHETRYSMGLFSFNRGIVHIPEEFVDELHPLRYRPFDIYEFLRFHDS 300

Query: 301 KGSKKFLYRIRDYCSI 317
              KK    I  +C I
Sbjct: 301 DEGKKTDGSITSFCGI 312

BLAST of CmaCh17G008320 vs. TrEMBL
Match: A0A061G9T4_THECC (2-oxoglutarate-dependent dioxygenase, putative OS=Theobroma cacao GN=TCM_028523 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.8e-88
Identity = 168/316 (53.16%), Postives = 215/316 (68.04%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MG ++Q ++PVID S+   L  GT  W S C  +R  LEE GCF   F++V L+LH++ F
Sbjct: 1   MGLQSQFKLPVIDFSE-GNLNPGTSTWVSACNDVRQALEEQGCFEARFDKVPLQLHDTVF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
               ELF LP E +++NTS K Y  YFG+Y  LPLYES+A++NPT     QSF NLMWPA
Sbjct: 61  ATAAELFDLPTELKMRNTSNKPYFDYFGQYRSLPLYESLAMDNPTTHSGTQSFTNLMWPA 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN+ F E+ Q FS L+A+LD+ + RM+ ESYGV     D  I ST  LLRF+KYR PE 
Sbjct: 121 -GNDRFRESAQSFSELVAELDRTVMRMLFESYGVGNYY-DYYIKSTNYLLRFYKYRKPEM 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +E+D GL  HTD T  SIIHQ  I GLQ++  D QW+ ++PS   SF VMAGDALMAWSN
Sbjct: 181 NESDTGLPPHTDRTLLSIIHQGRISGLQVKLKDGQWVGVQPSP-TSFAVMAGDALMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
           DRI  C H+VTMK +ETRYS GMFSF +G I + EELVD+ +PL+YK F+HF FL +D++
Sbjct: 241 DRIPPCYHQVTMKEKETRYSIGMFSFISGIIHILEELVDETHPLKYKSFDHFQFLHFDRS 300

Query: 301 KGSKKFLYRIRDYCSI 317
              KK    I+ +C +
Sbjct: 301 DEGKKSKCSIKAFCGV 312

BLAST of CmaCh17G008320 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 288.1 bits (736), Expect = 6.5e-78
Identity = 135/309 (43.69%), Postives = 199/309 (64.40%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           +VP++DL+  + L+  T  W S  +     LEE+GCF  V++ V+ +L +S F A +ELF
Sbjct: 9   KVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELF 68

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFS 127
            LP ET+ KN +EK YHGY G+   +PL+E + ++  T  + AQ F +LMWP QGN+ F 
Sbjct: 69  DLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWP-QGNDRFC 128

Query: 128 ETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHETDVGL 187
            T+  FS  +A+LD+++ RM+ E+YGV K   ++++ S   LL+F KY  P E  +    
Sbjct: 129 NTVHTFSNAVAELDRLVVRMIFENYGVEKH-YESHVGSKTYLLKFLKYLAPPESISMPAF 188

Query: 188 HAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDRIRSCR 247
             HTD TF SI+HQ+ + GL+++S D +WI L+     S+VVMAGD  M WSNDRIRSC 
Sbjct: 189 PQHTDKTFLSILHQNDVNGLEVKSKDGEWISLQLPPK-SYVVMAGDISMGWSNDRIRSCE 248

Query: 248 HRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAKGSKKFL 307
           HRVTM+ ++TRY+ G+FSF    +S+PEELVDD +PL YKPF++   + +   K  ++  
Sbjct: 249 HRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREAN 308

Query: 308 YRIRDYCSI 317
             ++ YC I
Sbjct: 309 STLKAYCGI 314

BLAST of CmaCh17G008320 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 272.3 bits (695), Expect = 3.7e-73
Identity = 135/308 (43.83%), Postives = 200/308 (64.94%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCV-VFEEVSLELHNSFFGAVDEL 67
           ++P +D S+ E L+ GT+ W ST + +R  LEE+GCF + + ++  L+L +  FG++ +L
Sbjct: 4   KIPTLDFSR-EDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDL 63

Query: 68  FQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHF 127
           F LP +T++KN  +K  +GY G+   LPL+ES+ I+N T L+A +SF  LMWP QGN HF
Sbjct: 64  FDLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWP-QGNEHF 123

Query: 128 SETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHETDVG 187
           SE + +++   A+LD+++TRMV +SY V K   D  I+ST  LLR  K R P      +G
Sbjct: 124 SECLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLG 183

Query: 188 LHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSNDRIRSC 247
              HTD +F +I+HQ  + GL++++ + + I++  S    F+V+AGDALMAWSNDR+ S 
Sbjct: 184 FVTHTDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSP 243

Query: 248 RHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAKGSKKF 307
           RH+V +  E  RYS GMF+F NGT+ VPEEL+D  +PL YKPF+H   L + +     K 
Sbjct: 244 RHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKS 303

Query: 308 LYRIRDYC 315
              I+ YC
Sbjct: 304 ECPIKAYC 308

BLAST of CmaCh17G008320 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 259.2 bits (661), Expect = 3.2e-69
Identity = 135/321 (42.06%), Postives = 200/321 (62.31%), Query Frame = 1

Query: 1   MGSETQA---RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHN 60
           MGSET     R+PVID S  + L+ G   W  T   ++  L+++G F   F+ +  EL  
Sbjct: 1   MGSETPLLPLRLPVIDFSN-KNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRK 60

Query: 61  SFFGAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLM 120
           S FGA++ELF LP++T+++N S+K +HGY G+Y  +PLYESM I++    +   +F   +
Sbjct: 61  SVFGALEELFDLPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKL 120

Query: 121 WPAQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRV 180
           WP QGN  FS TIQ FS  +++LD  I RM++ES+G+ K  ID ++ ST  LLR  KY+ 
Sbjct: 121 WP-QGNISFSTTIQSFSKKLSELDITIRRMIMESFGLDK-YIDEHLHSTNYLLRVMKYKG 180

Query: 181 PEEHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMA 240
           P+  ET VGL+AHTD    +I++Q+ + GL++Q+ D  WI ++P+   SF VM GD+L A
Sbjct: 181 PDTEETKVGLNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPT-QDSFTVMIGDSLYA 240

Query: 241 WSNDRIRSCRHRVTMKAEETRYSAGMFSFKNG--TISVPEELVDDANPLRYKPFNHFDFL 300
             N R+ S  HRV M   ETRYS G+FS       +S P+ELVD+ +P  +KPF+H +FL
Sbjct: 241 LLNGRLHSPYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFL 300

Query: 301 TYDKAKGSKKFLYRIRDYCSI 317
            +   +  ++    ++ YC I
Sbjct: 301 QFYYTEAGQRSQSALKTYCGI 317

BLAST of CmaCh17G008320 vs. TAIR10
Match: AT1G80320.1 (AT1G80320.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 238.4 bits (607), Expect = 5.9e-63
Identity = 129/323 (39.94%), Postives = 191/323 (59.13%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           M S  +  +P++D S  + L  GT  W +T   +R  +E  G F   F  VS +L ++  
Sbjct: 1   MDSHEEQCLPILDFSS-DKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLL 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRY-SFLPLYESMAIENPTQLDAAQSFANLMWP 120
             + E++ LP + +IKN + KA HGY         ++ES+ I+  T+L A + F+ L+WP
Sbjct: 61  AGMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWP 120

Query: 121 AQGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVT-KQLIDTNIDSTANLLRFFKYRVP 180
            QGN+ F +T   ++  MA+LD+ + RM+ ESYG+  K+   ++ +ST  LLR   YR  
Sbjct: 121 -QGNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQ 180

Query: 181 EEHETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAW 240
           +  E + G  +HTD +F SI+HQ+ +GGLQ++++  QW+   PS    FVV++G  L AW
Sbjct: 181 QNGEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTGQWVGFNPSP-TRFVVLSGMGLTAW 240

Query: 241 SNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTY- 300
           SNDRI++C H+V M A+E RYS G FSF  GTI  PEELVDD +PLRY PF H   L + 
Sbjct: 241 SNDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFY 300

Query: 301 ----DKAKGSKKFLYRIRDYCSI 317
               +  K S + L +I  YC +
Sbjct: 301 ESYINSLKKSSEDLLQI--YCGV 318

BLAST of CmaCh17G008320 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 227.6 bits (579), Expect = 1.0e-59
Identity = 120/305 (39.34%), Postives = 184/305 (60.33%), Query Frame = 1

Query: 8   RVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGAVDELF 67
           ++PVID S  + L+ G+  W      +   LE++GCF   F+++S+EL+ S F A+++LF
Sbjct: 13  QLPVIDFSD-QNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLF 72

Query: 68  QLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQGNNHFS 127
           +LPI T+ +N S K +HGY        LYES+ I +   L+    F   +WP  GN   S
Sbjct: 73  ELPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSIS 132

Query: 128 ETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVP------EEH 187
           ETI  FS  + +LD ++ RM++ES+G+ +  ID +++ST  L R  KY  P      ++ 
Sbjct: 133 ETIHLFSEQLVELDLMVRRMIMESFGI-ENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDE 192

Query: 188 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSND 247
           ET +GL +HTD    +I+HQ+ + GL++++ DD+WI ++PS   S +VM GD+L A  N 
Sbjct: 193 ETKLGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPS-QDSVLVMVGDSLCALLNG 252

Query: 248 RIRSCRHRVTMKAEETRYSAGMFSF-KNGT-ISVPEELVDDANPLRYKPFNHFDFLTYDK 305
           R+ S  HRV M  ++TRYS G+FS  K G  I  PEELVD  +P  +KPF + DFL + +
Sbjct: 253 RLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQ 310

BLAST of CmaCh17G008320 vs. NCBI nr
Match: gi|659122281|ref|XP_008461057.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 473.4 bits (1217), Expect = 3.1e-130
Identity = 224/315 (71.11%), Postives = 261/315 (82.86%), Query Frame = 1

Query: 3   SETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62
           ++++  +P IDLSKLE    GT+ WG  CK MR GLEEFGCF VV+++VSLELHNS   A
Sbjct: 5   AKSKVVIPTIDLSKLEV---GTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSIVEA 64

Query: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQG 122
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAI+NP QL + QSFANLMWP + 
Sbjct: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWPTRD 124

Query: 123 NNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEEHE 182
           NNHF  T++RFSTL+AKL+K++T+MVLESYGV+K + +  ++ST  LLR FKYRV E+ E
Sbjct: 125 NNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAEKDE 184

Query: 183 TDVGLHAHTDLTFFSIIHQHLIGGLQIQSL-DDQWIDLEPSGHCSFVVMAGDALMAWSND 242
           TDVGLH+HTDLTFFSIIHQHLI GLQIQSL DDQW+D+ PS HCSF+VMAGDALMAWSN+
Sbjct: 185 TDVGLHSHTDLTFFSIIHQHLISGLQIQSLNDDQWLDVHPSSHCSFIVMAGDALMAWSNE 244

Query: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAK 302
           RIRSCRHRV M+ EETRYS GMFSFKNGTI VP+  VDDANPLRYKPFNH+DFLTYDKA 
Sbjct: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKAN 304

Query: 303 GSKKFLYRIRDYCSI 317
            S   + RI+DYC +
Sbjct: 305 ASHNTISRIKDYCGL 316

BLAST of CmaCh17G008320 vs. NCBI nr
Match: gi|449436585|ref|XP_004136073.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus])

HSP 1 Score: 462.2 bits (1188), Expect = 7.1e-127
Identity = 217/315 (68.89%), Postives = 256/315 (81.27%), Query Frame = 1

Query: 3   SETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFFGA 62
           ++++  +P+IDLSKLE    GT+ WG  C+ MR GLE+FGCF VV+++VSLELHNS F A
Sbjct: 5   AKSKVVIPIIDLSKLEE---GTKEWGLACREMRFGLEQFGCFSVVYDKVSLELHNSIFEA 64

Query: 63  VDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPAQG 122
             ELF LPIETR+KNTSEK YHGYFG YSFLPLYESMAI+NP QL   QSFANLMWP   
Sbjct: 65  TKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLQPTQSFANLMWPTTE 124

Query: 123 NNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNI-DSTANLLRFFKYRVPEEH 182
           NNHF   ++RFSTL+AKL+ ++T+MVLESYGV+ +++   I +ST  LLR FKYRV E+ 
Sbjct: 125 NNHFCVAVERFSTLVAKLEHMVTKMVLESYGVSNKVVSEPIMESTNYLLRLFKYRVAEKD 184

Query: 183 ETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSND 242
           E DVGLH+HTDLTF SIIHQHLI GLQIQ LDDQW+++ PS HCSF VMAGDALMAWSN+
Sbjct: 185 ENDVGLHSHTDLTFLSIIHQHLISGLQIQLLDDQWLEVHPSSHCSFTVMAGDALMAWSNE 244

Query: 243 RIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKAK 302
           RIRSCRHRV M+ EETRYS GMFSFKNG I VP+E VDDANPLRYKPF+H+DFLTYDKA 
Sbjct: 245 RIRSCRHRVIMRGEETRYSIGMFSFKNGRIEVPQEFVDDANPLRYKPFHHYDFLTYDKAN 304

Query: 303 GSKKFLYRIRDYCSI 317
            S K + RI+DYC +
Sbjct: 305 ASHKTISRIKDYCGL 316

BLAST of CmaCh17G008320 vs. NCBI nr
Match: gi|1009175159|ref|XP_015868729.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba])

HSP 1 Score: 376.3 bits (965), Expect = 5.2e-101
Identity = 178/316 (56.33%), Postives = 242/316 (76.58%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGS+T+ +VPVIDL+K E L+ GT++W S CK +R   EE+GC  VV+++VS+EL +S F
Sbjct: 1   MGSQTEMKVPVIDLTK-ENLKPGTDSWISACKQVRYAFEEYGCVEVVYDKVSMELGSSMF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
             VD+LF LPIET+++NTS++ YH YFGRYSF+PLYES+ I+NPT L+ AQ F ++MWP 
Sbjct: 61  AVVDDLFNLPIETKMQNTSDRPYHSYFGRYSFIPLYESLGIDNPTTLEGAQHFTSIMWP- 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
           QGN+HF ET+Q +S L+A+++  +TRM+ +SYGV ++L +++++  + LLR F+YR    
Sbjct: 121 QGNDHFRETVQTYSKLVAEINGTVTRMLFDSYGV-ERLCESHMEWNSYLLRCFRYRTRRP 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
            E D+GL +HTD TF S +HQ+ I GLQI++ D QWID+EPSG  SF+V+AGD LMAWSN
Sbjct: 181 DEPDLGLWSHTDRTFISTLHQNQISGLQIKTKDGQWIDVEPSG-SSFLVLAGDVLMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
           DRI SC HRV MK  + RYS G+FSF NG I VPEELVDD  PLRYKPFN+FD+L + +A
Sbjct: 241 DRIPSCDHRVIMKENKIRYSLGLFSFNNGLIKVPEELVDDQFPLRYKPFNNFDYLRFTQA 300

Query: 301 KGSKKFLYRIRDYCSI 317
           + SKK+   I+ YC +
Sbjct: 301 EESKKYGCAIKAYCGV 312

BLAST of CmaCh17G008320 vs. NCBI nr
Match: gi|657971607|ref|XP_008377592.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Malus domestica])

HSP 1 Score: 374.4 bits (960), Expect = 2.0e-100
Identity = 173/316 (54.75%), Postives = 239/316 (75.63%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQ ++PVIDLS  E L+ GT+AW   CK ++  LEE+GCF  ++ ++ LELHNS F
Sbjct: 1   MGSETQPKLPVIDLSD-ENLKPGTDAWLLACKEIKDALEEYGCFEAIYHKIPLELHNSIF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
            A+++LF LP+ET+ + TS++ YH YFG+YSFLPLYES+ ++NPT ++ AQ F ++MWP 
Sbjct: 61  SAMEDLFGLPLETKKQKTSDRPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTSIMWP- 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
           QGN HF ET   FS L+A+L +++TRMV + YGV ++L D++++ST  LLR FKYR  EE
Sbjct: 121 QGNAHFCETAHSFSKLVAELSQMVTRMVFDMYGV-QRLFDSHMESTTYLLRCFKYRTQEE 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +ET+VGLH HTD TF SI++Q+ + GLQI + D QWID+ PS   SF+++AGDA MAWSN
Sbjct: 181 NETNVGLHPHTDKTFISILNQNQVNGLQIGTKDGQWIDVNPS-PSSFLILAGDAFMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
           DR+ SC H+VTMK  +TRYS G+F+FK+G I VPEEL+D+ +PL YKPF+HF FL +D+ 
Sbjct: 241 DRVPSCDHQVTMKENKTRYSLGLFAFKSGIIQVPEELIDEKHPLLYKPFDHFSFLDFDRT 300

Query: 301 KGSKKFLYRIRDYCSI 317
             ++K    I+ YC +
Sbjct: 301 PEARKLKCSIKAYCGV 312

BLAST of CmaCh17G008320 vs. NCBI nr
Match: gi|645244882|ref|XP_008228621.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Prunus mume])

HSP 1 Score: 369.8 bits (948), Expect = 4.8e-99
Identity = 179/316 (56.65%), Postives = 233/316 (73.73%), Query Frame = 1

Query: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60
           MGSETQ ++PVIDLS  E L+ GTEAW    K ++  LEE+GCF  VF +V L+LHNS F
Sbjct: 1   MGSETQPKLPVIDLSN-ENLKPGTEAWVLASKHVKYALEEYGCFEAVFHKVPLQLHNSIF 60

Query: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120
             V++LF LPIET+ + TSE+ YH YFG+YSFLPLYES+ ++NPT ++ AQ F  +MWPA
Sbjct: 61  SVVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTKIMWPA 120

Query: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180
            GN+HF E+ Q FS L+A+LD+++TRMV + YGV ++L D+++ ST  L R FKYR  +E
Sbjct: 121 -GNDHFCESAQSFSKLVAELDQMVTRMVFDIYGV-ERLYDSHMASTTYLFRCFKYREQKE 180

Query: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSLDDQWIDLEPSGHCSFVVMAGDALMAWSN 240
           +ET+VGL  H D TF SI+HQ  + GLQ++S D QWID EPS   SF+VMAGDA MAWSN
Sbjct: 181 NETNVGLRPHKDKTFTSILHQSQVHGLQVRSKDCQWIDAEPS-PSSFLVMAGDAFMAWSN 240

Query: 241 DRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLTYDKA 300
           DR+RSC H+V MK  +TRYS G+FSF +G + VPEEL DD +PL YKPF+HF FL +D+ 
Sbjct: 241 DRVRSCDHQVIMKENKTRYSLGLFSFNSGILEVPEELTDDQHPLLYKPFDHFGFLRFDQT 300

Query: 301 KGSKKFLYRIRDYCSI 317
             +KK    I+ YC +
Sbjct: 301 PEAKKSECPIKAYCGV 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AOP1V_ARATH4.9e-5939.93Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha... [more]
AOP1L_ARATH1.1e-5839.47Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
AOP1C_ARATH1.9e-5839.34Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP2V_ARATH3.4e-2836.602-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP2L_ARATH9.9e-2836.082-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
Match NameE-valueIdentityDescription
M5WSX6_PRUPE3.4e-9956.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008973mg PE=3 SV=1[more]
V4ST91_9ROSI2.2e-9052.83Uncharacterized protein OS=Citrus clementina GN=CICLE_v10027420mg PE=3 SV=1[more]
V4SN85_9ROSI2.9e-9050.95Uncharacterized protein (Fragment) OS=Citrus clementina GN=CICLE_v10027182mg PE=... [more]
A0A067F428_CITSI2.9e-9050.95Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g037660mg PE=3 SV=1[more]
A0A061G9T4_THECC7.8e-8853.162-oxoglutarate-dependent dioxygenase, putative OS=Theobroma cacao GN=TCM_028523 ... [more]
Match NameE-valueIdentityDescription
AT1G52800.16.5e-7843.69 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52790.13.7e-7343.83 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52820.13.2e-6942.06 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G80320.15.9e-6339.94 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.11.0e-5939.34 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
Match NameE-valueIdentityDescription
gi|659122281|ref|XP_008461057.1|3.1e-13071.11PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi... [more]
gi|449436585|ref|XP_004136073.1|7.1e-12768.89PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis sativus][more]
gi|1009175159|ref|XP_015868729.1|5.2e-10156.33PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba][more]
gi|657971607|ref|XP_008377592.1|2.0e-10054.75PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Malus domestica][more]
gi|645244882|ref|XP_008228621.1|4.8e-9956.65PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Prunus mume][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G008320.1CmaCh17G008320.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 175..265
score: 6.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 166..269
score: 11
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..88
score: 5.1
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 4..297
score: 2.5
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..308
score: 9.9E
NoneNo IPR availablePANTHERPTHR10209:SF1742-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 1..308
score: 9.9E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 7..297
score: 2.06

The following gene(s) are paralogous to this gene:

None