Lsi07G004900 (gene) Bottle gourd (USVL1VR-Ls)

Overview
NameLsi07G004900
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description2-oxoglutarate-dependent dioxygenase
Locationchr07 : 5178106 .. 5178516 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

mRNA sequence

ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

Coding sequence (CDS)

ATGGAAATTCAAACTTTAGCAAAGTGTGAAGTTCCTTTGATAAAATTCTCCATTGAATTAAATGATTATAATTTTGATGATCCTCAAAGCACCAAATGGTTAGAAAGATGCAGCCAAGTGAGAGTTGCTCTTGAAGAATATGGCTGTTTCTTAGCTGAGTATGATGAGATCAGTGCCCCACAAGAGCTTTATGAAGCAATTTTGAAGGGCTTGAAAGTGCTTTTTGATTTGCCATTAGAAACAAAAATGAAGAATGTTTCTGATAAGCCATACCATGGATTTTTGGACCATACGACTCCCTTTGTTCTTCCTCTTCATCAAAGCCTTGGCATTGAAAATGCAACATCTTTTCAAGCTGTTGAATCTTTTGTCAACCTCTTCTGGCCTTCTGGAAATCATCATTTCTGGTAA

Protein sequence

MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHFW
Homology
[Close]
BLAST of Lsi07G004900 vs. Swiss-Prot
Match: AOP2V_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 2.5e-20
Identity = 50/106 (47.17%), Postives = 67/106 (63.21%), Query Frame = 1 

Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR ALE++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALEDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + V  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENVNEFTQQLWPDGD 117

[Close]
BLAST of Lsi07G004900 vs. Swiss-Prot
Match: AOP2C_ARATH (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.3e-20
Identity = 49/106 (46.23%), Postives = 67/106 (63.21%), Query Frame = 1 

Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR ALE++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALEDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + +  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENINEFTQQLWPHGD 117

[Close]
BLAST of Lsi07G004900 vs. Swiss-Prot
Match: AOP2L_ARATH (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 9.5e-20
Identity = 48/106 (45.28%), Postives = 67/106 (63.21%), Query Frame = 1 

Query: 27  DPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNV 86
           +P S+KW E  S VR AL+++GCF A YD++S   EL E+I+K ++ LF LP+ETK +NV
Sbjct: 19  EPGSSKWAEVRSDVRKALQDFGCFEASYDKVSL--ELQESIMKTMEELFALPVETKQRNV 78

Query: 87  SDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGN 133
             KPY G+L+H       L +SLGI NA   + +  F    WP G+
Sbjct: 79  CPKPYVGYLNHNN-----LSESLGISNANILENINEFTQQLWPHGD 117

[Close]
BLAST of Lsi07G004900 vs. Swiss-Prot
Match: AOP1C_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AOP1 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.3e-18
Identity = 46/120 (38.33%), Postives = 72/120 (60.00%), Query Frame = 1 

Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWP 119

[Close]
BLAST of Lsi07G004900 vs. Swiss-Prot
Match: AOP1L_ARATH (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 5.2e-18
Identity = 46/120 (38.33%), Postives = 73/120 (60.83%), Query Frame = 1 

Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI++A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGIDDANVLEKVNDFTQQLWP 119

[Close]
BLAST of Lsi07G004900 vs. TrEMBL
Match: A0A0A0LBU1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G555690 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 4.3e-43
Identity = 96/148 (64.86%), Postives = 107/148 (72.30%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTK-WLERCSQVRVALEEYGCFLAEYDE--- 60
           M I  LAK EVP+IK S      +  DP+S K WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  ------ISAPQ---ELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQ 120
                  S+P+     YE I KGLK LFDLPLETK+KNVSDKPYHGFLD  TPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENATSFQAVESFVNLFWPSGNHHF 136
           SLGIENA+S++AV+ FVNL WPSGNHHF
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHF 148

[Close]
BLAST of Lsi07G004900 vs. TrEMBL
Match: W9STP2_9ROSA (Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.8e-26
Identity = 68/136 (50.00%), Postives = 92/136 (67.65%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAP 60
           M +++L K  +P I FS    + N + P ++ W   C+ VR ALEEYGCF+A YD+IS  
Sbjct: 1   MVVKSLPK--IPTIDFS----EGNLN-PGTSSWFSVCNDVRRALEEYGCFVALYDKIS-- 60

Query: 61  QELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAV 120
           Q+L  ++   LK LFDLP E KMKNVSDKPY G++ +  P + PLH+S+GIENAT+ +  
Sbjct: 61  QQLRTSMFSELKELFDLPTEIKMKNVSDKPYFGYIGNH-PSIPPLHESMGIENATTLEGG 120

Query: 121 ESFVNLFWP-SGNHHF 136
           ESF N+ WP  GN+HF
Sbjct: 121 ESFTNIIWPKGGNNHF 126

[Close]
BLAST of Lsi07G004900 vs. TrEMBL
Match: D7U0R2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05350 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-25
Identity = 59/130 (45.38%), Postives = 86/130 (66.15%), Query Frame = 1 

Query: 7   AKCEVPLIKFSIE-LNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYE 66
           +  ++P++ FS E LN      P S+ W   C  V +ALEE+GCF+A YD++  P EL  
Sbjct: 5   SSAKIPVVDFSKENLN------PGSSSWSATCKDVLLALEEFGCFIAVYDKV--PSELQN 64

Query: 67  AILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVN 126
            +   L+ LF+LP ETKM+N  +KPYHG++   +   LPLH+S+GI+NAT+ + ++SF N
Sbjct: 65  KLFSALEELFNLPTETKMQNAHNKPYHGYVGQHSR--LPLHESIGIDNATTLEGMQSFAN 124

Query: 127 LFWPSGNHHF 136
           L WP+GN HF
Sbjct: 125 LMWPAGNDHF 124

[Close]
BLAST of Lsi07G004900 vs. TrEMBL
Match: A5AE66_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030994 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.4e-25
Identity = 59/130 (45.38%), Postives = 85/130 (65.38%), Query Frame = 1 

Query: 7   AKCEVPLIKFSIE-LNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYE 66
           +  ++P++ FS E LN      P S+ W   C  V +ALEE+GCF+A YD++  P EL  
Sbjct: 5   SSAKIPVVDFSKENLN------PGSSSWSATCKDVLLALEEFGCFIAVYDKV--PSELQN 64

Query: 67  AILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVN 126
            +   L+ LF+LP ETKM+N  +KPYHG++   +   LPLH+S+GI+NAT+ +  +SF N
Sbjct: 65  KLFSALEELFNLPTETKMQNAHNKPYHGYVGQHSX--LPLHESIGIDNATTLEGXQSFAN 124

Query: 127 LFWPSGNHHF 136
           L WP+GN HF
Sbjct: 125 LMWPAGNDHF 124

[Close]
BLAST of Lsi07G004900 vs. TrEMBL
Match: F6HX49_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05320 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.5e-24
Identity = 59/126 (46.83%), Postives = 80/126 (63.49%), Query Frame = 1 

Query: 10  EVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 69
           ++P+I FS E    N   P S  W   C  VR ALEEYGCF+A Y+++  P EL+  I  
Sbjct: 8   KLPIIDFSEE----NLK-PGSDSWFSVCHDVRHALEEYGCFMAYYNQV--PLELHNKIFN 67

Query: 70  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 129
            L  LFDLP E K+KN SDKP HG++     +  PLH+S+GI++AT+ + ++SF  L WP
Sbjct: 68  ALGELFDLPTEIKVKNTSDKPSHGYIGQIPAY--PLHESMGIDHATNLEEIQSFAKLMWP 124

Query: 130 SGNHHF 136
           +GN HF
Sbjct: 128 AGNEHF 124

[Close]
BLAST of Lsi07G004900 vs. TAIR10
Match: AT1G52790.1 (AT1G52790.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 105.1 bits (261), Expect = 3.4e-23
Identity = 52/116 (44.83%), Postives = 70/116 (60.34%), Query Frame = 1 

Query: 22  DYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPL 81
           D++ +D  P +  W      +R ALEEYGCF+ +  +   P +L + +   L  LFDLP 
Sbjct: 9   DFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKD-KTPLDLLDRVFGSLVDLFDLPT 68

Query: 82  ETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHF 136
           +TKMKN  DKP +G++       LPLH+SLGI+NATS +A  SF  L WP GN HF
Sbjct: 69  QTKMKNKYDKPLNGYVGQIP--ALPLHESLGIDNATSLEATRSFTGLMWPQGNEHF 121

[Close]
BLAST of Lsi07G004900 vs. TAIR10
Match: AT1G52800.1 (AT1G52800.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 101.7 bits (252), Expect = 3.7e-22
Identity = 47/108 (43.52%), Postives = 68/108 (62.96%), Query Frame = 1 

Query: 28  PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILKGLKVLFDLPLETKMKNVS 87
           P ++ W     +   ALEEYGCFLA YD ++  Q+L ++I    + LFDLP ETK KNV+
Sbjct: 23  PNTSTWRSVSREACEALEEYGCFLAVYDGVT--QQLDDSIFAAAEELFDLPTETKKKNVN 82

Query: 88  DKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHF 136
           +KPYHG++      V+PLH+ LG++  T+ +  + F +L WP GN  F
Sbjct: 83  EKPYHGYVGQMP--VIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRF 126

[Close]
BLAST of Lsi07G004900 vs. TAIR10
Match: AT4G03070.1 (AT4G03070.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-19
Identity = 46/120 (38.33%), Postives = 72/120 (60.00%), Query Frame = 1 

Query: 12  PLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAILK 71
           P + F + + D++  +  P S+KW E  + V  ALE+YGCF A +D++S   EL  ++ +
Sbjct: 8   PSVSFQLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSV--ELNRSVFE 67

Query: 72  GLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLFWP 130
            ++ LF+LP+ TK +NVS KP+HG+L H       L++SLGI +A   + V  F    WP
Sbjct: 68  AMEDLFELPIPTKQRNVSSKPFHGYLCHN------LYESLGINDANVLEKVNDFTQQLWP 119

[Close]
BLAST of Lsi07G004900 vs. TAIR10
Match: AT1G52820.1 (AT1G52820.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 91.3 bits (225), Expect = 5.0e-19
Identity = 46/128 (35.94%), Postives = 79/128 (61.72%), Query Frame = 1 

Query: 10  EVPLIKFSIELNDYNFDD--PQSTKWLERCSQVRVALEEYGCFLAEYDEISAPQELYEAI 69
           E PL+   + + D++  +  P   +W    + V+ AL++YG F A +D I  P EL +++
Sbjct: 4   ETPLLPLRLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRI--PFELRKSV 63

Query: 70  LKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQAVESFVNLF 129
              L+ LFDLPL+TK++NVS KP+HG++      ++PL++S+GI+++   + V++F    
Sbjct: 64  FGALEELFDLPLQTKLRNVSKKPFHGYVGQYP--MVPLYESMGIDDSDIAEKVDAFTEKL 123

Query: 130 WPSGNHHF 136
           WP GN  F
Sbjct: 124 WPQGNISF 127

[Close]
BLAST of Lsi07G004900 vs. TAIR10
Match: AT1G52810.1 (AT1G52810.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 82.4 bits (202), Expect = 2.3e-16
Identity = 47/136 (34.56%), Postives = 77/136 (56.62%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKF-SIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISA 60
           + ++T    ++P+I F S +L       P + +W    + VR ALEEYGCF A +D++  
Sbjct: 2   VSLETTLALQLPVIDFTSRDLK------PGTIQWDSVRADVRRALEEYGCFEALFDKV-- 61

Query: 61  PQELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQSLGIENATSFQA 120
           P EL +A+    + +F LPLETK + VS + Y G++       LPL + +G++ A +   
Sbjct: 62  PLELRKAVFDASEEVFQLPLETKKRVVSKRKYRGYVGQIP--TLPLFEVMGVDFAENPDK 121

Query: 121 VESFVNLFWPSGNHHF 136
           V +F +  WP GN++F
Sbjct: 122 VNAFTHKLWPQGNNNF 127

[Close]
BLAST of Lsi07G004900 vs. NCBI nr
Match: gi|778688674|ref|XP_011652809.1| (PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP2-like [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 4.3e-44
Identity = 97/149 (65.10%), Postives = 108/149 (72.48%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTK-WLERCSQVRVALEEYGCFLAEYDE--- 60
           M I  LAK EVP+IK S      +  DP+S K WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  ------ISAPQ---ELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQ 120
                  S+P+     YE I KGLK LFDLPLETK+KNVSDKPYHGFLD  TPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENATSFQAVESFVNLFWPSGNHHFW 137
           SLGIENA+S++AV+ FVNL WPSGNHHFW
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHFW 149

[Close]
BLAST of Lsi07G004900 vs. NCBI nr
Match: gi|700203009|gb|KGN58142.1| (hypothetical protein Csa_3G555690 [Cucumis sativus])

HSP 1 Score: 182.2 bits (461), Expect = 6.1e-43
Identity = 96/148 (64.86%), Postives = 107/148 (72.30%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTK-WLERCSQVRVALEEYGCFLAEYDE--- 60
           M I  LAK EVP+IK S      +  DP+S K WLER  QVR ALEEYGCFL EYD+   
Sbjct: 1   MGIDCLAKLEVPIIKLSNVDKKDDDSDPESRKLWLERSKQVRDALEEYGCFLGEYDDDES 60

Query: 61  ------ISAPQ---ELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHTTPFVLPLHQ 120
                  S+P+     YE I KGLK LFDLPLETK+KNVSDKPYHGFLD  TPFVLPLHQ
Sbjct: 61  SCSCNSSSSPKLNSNNYEEIFKGLKELFDLPLETKIKNVSDKPYHGFLDCRTPFVLPLHQ 120

Query: 121 SLGIENATSFQAVESFVNLFWPSGNHHF 136
           SLGIENA+S++AV+ FVNL WPSGNHHF
Sbjct: 121 SLGIENASSYEAVQFFVNLLWPSGNHHF 148

[Close]
BLAST of Lsi07G004900 vs. NCBI nr
Match: gi|659093708|ref|XP_008447674.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo])

HSP 1 Score: 135.2 bits (339), Expect = 8.6e-29
Identity = 67/97 (69.07%), Postives = 74/97 (76.29%), Query Frame = 1 

Query: 50  FLAEYDE--------ISAPQEL---YEAILKGLKVLFDLPLETKMKNVSDKPYHGFLDHT 109
           FL EYD+         S+  +L   YE I KGLK LFD PLETKMKNVSDKPYHGFLD  
Sbjct: 109 FLCEYDDDESSRCCNSSSSSKLDSNYEEIFKGLKELFDFPLETKMKNVSDKPYHGFLDSR 168

Query: 110 TPFVLPLHQSLGIENATSFQAVESFVNLFWPSGNHHF 136
            PFVLPLHQSLGIENA+S++AV+SFVNL WPSGNHHF
Sbjct: 169 APFVLPLHQSLGIENASSYEAVQSFVNLLWPSGNHHF 205

[Close]
BLAST of Lsi07G004900 vs. NCBI nr
Match: gi|1009177450|ref|XP_015869979.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Ziziphus jujuba])

HSP 1 Score: 129.4 bits (324), Expect = 4.7e-27
Identity = 70/139 (50.36%), Postives = 90/139 (64.75%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAP 60
           M  Q LAK  +P + FS E    N   P ++ W       R ALE+YGCF+A YD++S  
Sbjct: 1   MGAQPLAK--LPAVDFSKE----NLK-PGTSCWSSTAIDARQALEQYGCFVAIYDKMSP- 60

Query: 61  QELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFL---DHTTPFVLPLHQSLGIENATSF 120
            +L+++I + LK LFDLP E KMKN+SDKPY G+      TTP V  LH+SLGIEN T+ 
Sbjct: 61  -QLHDSIFRELKELFDLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTL 120

Query: 121 QAVESFVNLFWPSGNHHFW 137
           +AV+SF NL WP+GNH FW
Sbjct: 121 EAVQSFTNLMWPAGNHSFW 130

[Close]
BLAST of Lsi07G004900 vs. NCBI nr
Match: gi|1009175157|ref|XP_015868728.1| (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba])

HSP 1 Score: 125.9 bits (315), Expect = 5.2e-26
Identity = 70/138 (50.72%), Postives = 89/138 (64.49%), Query Frame = 1 

Query: 1   MEIQTLAKCEVPLIKFSIELNDYNFDDPQSTKWLERCSQVRVALEEYGCFLAEYDEISAP 60
           M  Q LAK  +P I FS E    N   P ++ W       R ALE+YGCF+A YD++S  
Sbjct: 1   MGAQPLAK--LPAIDFSKE----NLK-PGTSCWSSTAIDARQALEQYGCFVAIYDKMSP- 60

Query: 61  QELYEAILKGLKVLFDLPLETKMKNVSDKPYHGFL---DHTTPFVLPLHQSLGIENATSF 120
            +L+++I + LK LFDLP E KMKN+SDKPY G+      TTP V  LH+SLGIEN T+ 
Sbjct: 61  -QLHDSIFRELKELFDLPTEIKMKNISDKPYFGYAAGNSATTPHVPSLHESLGIENTTTL 120

Query: 121 QAVESFVNLFWPSGNHHF 136
           +AV+SF NL WP+GNH F
Sbjct: 121 EAVQSFTNLMWPAGNHSF 129

The following BLAST results are available for this feature:
BLAST of Lsi07G004900 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AOP2V_ARATH2.5e-2047.172-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP2C_ARATH4.3e-2046.23Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali... [more]
AOP2L_ARATH9.5e-2045.282-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana GN=AOP2 PE=2 S... [more]
AOP1C_ARATH2.3e-1838.33Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana GN=AO... [more]
AOP1L_ARATH5.2e-1838.33Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana GN=... [more]
BLAST of Lsi07G004900 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LBU1_CUCSA4.3e-4364.86Uncharacterized protein OS=Cucumis sativus GN=Csa_3G555690 PE=4 SV=1[more]
W9STP2_9ROSA4.8e-2650.00Gibberellin 3-beta-dioxygenase 4 OS=Morus notabilis GN=L484_019467 PE=3 SV=1[more]
D7U0R2_VITVI1.1e-2545.38Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05350 PE=3 SV=... [more]
A5AE66_VITVI1.4e-2545.38Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030994 PE=3 SV=1[more]
F6HX49_VITVI4.5e-2446.83Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g05320 PE=3 SV=... [more]
BLAST of Lsi07G004900 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G52790.13.4e-2344.83 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52800.13.7e-2243.52 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G03070.11.3e-1938.33 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52820.15.0e-1935.94 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G52810.12.3e-1634.56 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of Lsi07G004900 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778688674|ref|XP_011652809.1|4.3e-4465.10PREDICTED: 2-oxoglutarate-dependent dioxygenase AOP2-like [Cucumis sativus][more]
gi|700203009|gb|KGN58142.1|6.1e-4364.86hypothetical protein Csa_3G555690 [Cucumis sativus][more]
gi|659093708|ref|XP_008447674.1|8.6e-2969.07PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucumis melo][more]
gi|1009177450|ref|XP_015869979.1|4.7e-2750.36PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1.2 [Ziziphus jujuba... [more]
gi|1009175157|ref|XP_015868728.1|5.2e-2650.72PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 [Ziziphus jujuba][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR027443IPNS-like
IPR026992DIOX_N
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
cellular_component GO:0005737 cytoplasm
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G004900.1Lsi07G004900.1mRNA


InterPro
Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 12..94
score: 1.
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 24..98
score: 5.
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 10..135
score: 3.0
NoneNo IPR availablePANTHERPTHR10209:SF1742-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 10..135
score: 3.0
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 24..135
score: 1.44

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Lsi07G004900Csa7G434950Cucumber (Chinese Long) v2culsiB532
Lsi07G004900Csa3G555690Cucumber (Chinese Long) v2culsiB231
Lsi07G004900CSPI03G25170Wild cucumber (PI 183967)cpilsiB230
Lsi07G004900CmoCh17G008030Cucurbita moschata (Rifu)cmolsiB349
Lsi07G004900Cla012687Watermelon (97103) v1lsiwmB438
Lsi07G004900MELO3C023380Melon (DHL92) v3.5.1lsimeB430
Lsi07G004900ClCG07G012930Watermelon (Charleston Gray)lsiwcgB406
Lsi07G004900MELO3C030548.2Melon (DHL92) v3.6.1lsimedB456
Lsi07G004900MELO3C023381.2Melon (DHL92) v3.6.1lsimedB451
Lsi07G004900Cla97C07G139750Watermelon (97103) v2lsiwmbB405
Lsi07G004900Cla97C09G164600Watermelon (97103) v2lsiwmbB420
Lsi07G004900Bhi09G000062Wax gourdlsiwgoB577
Lsi07G004900Bhi01G002663Wax gourdlsiwgoB557
Lsi07G004900Cp4.1LG02g14620Cucurbita pepo (Zucchini)cpelsiB472
Lsi07G004900Cp4.1LG12g07740Cucurbita pepo (Zucchini)cpelsiB127
Lsi07G004900CsGy7G019260Cucumber (Gy14) v2cgyblsiB502
Lsi07G004900CsGy3G023650Cucumber (Gy14) v2cgyblsiB219
Lsi07G004900Carg04251Silver-seed gourdcarlsiB182
Lsi07G004900Carg09558Silver-seed gourdcarlsiB939
Lsi07G004900CsaV3_3G026700Cucumber (Chinese Long) v3cuclsiB252
Lsi07G004900CsaV3_7G032850Cucumber (Chinese Long) v3cuclsiB568
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Lsi07G004900Lsi02G027510Bottle gourd (USVL1VR-Ls)lsilsiB117
The following block(s) are covering this gene:
GeneOrganismBlock
Lsi07G004900Bottle gourd (USVL1VR-Ls)lsilsiB167
Lsi07G004900Cucumber (Gy14) v1cgylsiB266
Lsi07G004900Cucurbita maxima (Rimu)cmalsiB256
Lsi07G004900Cucurbita maxima (Rimu)cmalsiB450
Lsi07G004900Cucurbita maxima (Rimu)cmalsiB736
Lsi07G004900Cucurbita maxima (Rimu)cmalsiB845
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB137
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB174
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB244
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB437
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB727
Lsi07G004900Cucurbita moschata (Rifu)cmolsiB829
Lsi07G004900Cucurbita pepo (Zucchini)cpelsiB276
Lsi07G004900Cucurbita pepo (Zucchini)cpelsiB516
Lsi07G004900Wild cucumber (PI 183967)cpilsiB136
Lsi07G004900Wild cucumber (PI 183967)cpilsiB229
Lsi07G004900Wild cucumber (PI 183967)cpilsiB546
Lsi07G004900Cucumber (Chinese Long) v2culsiB137
Lsi07G004900Cucumber (Chinese Long) v2culsiB230
Lsi07G004900Melon (DHL92) v3.5.1lsimeB435
Lsi07G004900Melon (DHL92) v3.5.1lsimeB436
Lsi07G004900Melon (DHL92) v3.5.1lsimeB442
Lsi07G004900Watermelon (Charleston Gray)lsiwcgB387
Lsi07G004900Watermelon (Charleston Gray)lsiwcgB418
Lsi07G004900Watermelon (97103) v1lsiwmB426
Lsi07G004900Watermelon (97103) v1lsiwmB432
Lsi07G004900Melon (DHL92) v3.6.1lsimedB455
Lsi07G004900Melon (DHL92) v3.6.1lsimedB461
Lsi07G004900Silver-seed gourdcarlsiB109
Lsi07G004900Silver-seed gourdcarlsiB520
Lsi07G004900Silver-seed gourdcarlsiB685
Lsi07G004900Cucumber (Chinese Long) v3cuclsiB148
Lsi07G004900Watermelon (97103) v2lsiwmbB417
Lsi07G004900Wax gourdlsiwgoB568