Carg04109 (gene) Silver-seed gourd

NameCarg04109
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
DescriptionPectin lyase-like superfamily protein
LocationCucurbita_argyrosperma_scaffold_044 : 219509 .. 223094 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCGCAAGGCACACGATCTCCCTGCAGTTCTTTCAAGTAACGCCCAATCTCAACCTCCTCGCCGGTCTGGCGGTCCGACGAGCTTCCGTTGCCGGAGCGATGGCCGATTTATTTCTCTTTTCCATTCCTGCGACGGTTAACAACCAGAGACTTAGTGGGGGGAAAGGAGTTAGCGACAGTTAACAATGGCGAATTCAGAATCGCCTCCATGTTCTCAGCATCTCCGATTGCGAATTTAAAGCCAAATACTCTTCCATTCACGAATCGGTAAGTTAAAACAATTTCTTCCGATTTCAATTTCACTGAGCAGAAGCAAGGTTGTTAAGCTATTAGCATGAATTTCCCCTTTCGCACCACACTGATCTTGGTGCTCTCATCTTCCTGTTTTAGCTCAGTTCTTAGTAGAGGAATATTGGAAGCAGACATTTCAGGAAGCTTATTGCGTTACATAGCTAAGAGTTCTCATGTTTTCAGTGTTAGAAGCTTTGGTGCTCGAGCAAATGGCTTAACTGATGATAGCAATGTCTGTTCTATATTTGTTTGTGTTGATTTTCCTTGAGATTTTATTTGATGAACCGAATTCCTTAATCTCTATTTCTCCCTTCACCCGTTGAGCCTCGTCGCTGCTGCAATTTCCTTCAATTTTTTGTGCCATTTTTTTGCTTTTCCGTTAAATGCATTGTTCATGAGGATTTCCATGAGTCTGTCAGTCACATGTGTTCTGCTACTTAGCTCAATTTCTTGCTTGCTGGAAATGAAAAACCAGTTTCCTTCCAAATGGTGAGTTTGATTCATTACTTTATGTTTGATTCAATCCTCTTAAAAGGGGTTTTAGGTGTTCCCCTAACAATCACAGGGTTAAAAGTTACTATATATGTAAGATTTCTCATACACCAATTTGTTTTGTTTTCTAAACTCAACAATATATTATGATGTTCTAGAGTAGGAAGTGGTTTTGGTCCTGCAAAAAATGCTCTCAAACACACCCTTAAAAGAGTAAAAGATTAAGAACACTTTTCAGAAGTTTTCATTTGCTTGTGATTCAGGCCTTCTCAGAAACATGGAGGATGGCCTGTGGTTCAGCTGGAGTGGTAACTTTCCTGATTCCAAAAGGAACCTATTTGGTTGGTCCAGTTAAATTTTCAGGGCCTTGCAAAAATGTGTCGTCTCTAACGGTTAAAATGAAGGCAAGGGTCTAATCTTCTACGTATTTTCGTCTTTTTACGTCGAATCCGTTTGGTTTGAGCTCGAATTTTCTTTTTCAGGGATATTTGAAGGCATCAACAGACCTGTCAAAGTATGGTTCTGGTGCAGGCTGGGTTGAGTTCAGATGGATGGAGGGGCTAACGTTGACTGGAGGTGGAACCTTCGATGGCCGAGGCGCTGAGGCATGGCCATTCAACAACTGCCTTGATGACCACAATTGCAATCTCCTTCCTACTGTAAGTTCCATTCCACATTTTGTTCTGTTTGATTCTTAACCAATTCGTGTCTTGAAACATTAGAAATTGTAAGGACTAGTGAAATTATTTCAAAGAATCATTTCAAACTCATGCCTTAAAATATTAGGATTTGCAAAACTGTTCTGTTGCTGTTGTTGTTGCTGCCATTTGTAACATCAATATGATTCTTACAATGATTCAGTTCTTTTTCAGAACTTGAAATTCGTGGCTATGAATAACACGGTTGTTCGACGCATAACTTCGCTAAACAGCAAGTTCTTTCACTTGGCTTTAGTACAATGCAAGGGGTTCAGAGGTAGCAATCTCAAGATTTCAGCTCCAGCAAATAGTCCCAACACGGATGGTATCCACATCGACCGAAGCTCTAACATCCATCTTTCTCGATCACATATTCAAACTGGCGACGACTGCATATCCATTGGACAAGGGAACTCTCAAGTCACAATCACCGGCATCGATTGTGGACCGGGTCATGGGATCAGGTACGTAGATTGCTGCCAACAAGTATTATGCTTCTGTCATCATGAACTTGTACTCAGCTTACTAGAAACAGCATTCTGGACAACATGTGTTTTGTTCATTGCAGTGTTGGAAGCTTGGGAAGATATCAAAATGAAGGAGATGTAAGCGGATTAGTAGTTAAAAATTGTAGCCTCACAGGTACCACCAATGGGATCAGAATCAAGACATGGCCAAATTCTCCTGGCAGCAGCTCAGCCACAAACATGACATTTAAGAACTTCGCCATGAACAATGTGTCCAATCCAATTATCATTGATCAATCGTACTGTCCATTCAGATCTTGTTCTTTTAAGGTATGCCAGTGCTCTCTCAACATATATATTTCTTTTTTCAAAAAAGTGATTGCAAAGTTTTCTTCACGTGAGAACGGTAATGGTATAATATTTGTTAAGTTTATATCTCATTTTAGTCCTTAGACTTTCAAGTTCGTTTATATCTCATTTTAGTCCTTAACTTTCAAGTTCGTTTATATCTCATTTTAGTCCTTAACTTTCAAGTTCGTTTATATCTCGTTTTTAGTTCTTAGACTTTCAAATTCGTTTATATCTCGTTTTAGTTCTTAGACTTTTAAGTTCGTTTATATCTCATTTTAGTCCATAGACTTTTAAGTTCGTTTATATTTCATTTTAGTCCTTAGACTTTCAAGTTGGTTTTTATCTCATTTTAGTTCTTAGACTTTCAAGTTCGTTTATATCTCGTTTTTAGTTCTTAGACTTTCAAATTCGTTTATATCTCATTTTAGTTCTTAGACTTTTAAGTTCGTTTATATCTCATTTTAGTCCTTAGACTTTTAAGTTCGTTTATATCTCATTTTAGTCCTTACTTTCAAGTTCGTTTATATCTCATTTTAGTCCTTAGACTCTCAAGTTCGTTTATATCTCATTTTAGTCCTCAGACTCTCAAGTTCGTTTATATCTCATTTTAGTCCTCAGACTCTCAAGTTCGTTTATATCTCATTTTAGTCCTCAGACTTTCAAGTTCGTTTATATCTCATTTTAGTCCTTAGACTTTCAAGTTCGTTTTGTCTCACTCTCTGAAGTTGCAAATTTGTTCAATCTTCTATTAAGTCTTTTAGTTGCCGAATTTTTAGCTATTTTGATACCTACATTTTTCTAGTCCGTGGACTTTCAAAAAGATAACTATTGAAAGTTTGGAGACACCAAAATAGACATTCTCAAAACTTTTGAACGAAAATGGACATTTTGAAATTTGTAAAACCAAAAATAGAATAAACAAGAAAGTTTATGGACAAAAAAGATACGATCTTAACATAATTTTTTTGAAAGTTCGGTTCATAAAAGATCATTTCCTAAACAAATTTCAAAATCATTGCAAAATATGATTATATGAACGTGATTCATTTGAACAGGCACCATCTCTAGTGAAGCTGAGTGATATTTACTTCAAGAACATAAAAGGAACCTCTTCCTCTAAGGTGGCTGTGGCATTAGAATGCAGCAAGGGATTCCCATGTCAGAATGTGAACCTTGAAAATGTTCACTTGCTTCTTTCATCAGGTCAAAATCACCCTTCTTCTACTTGCAAATATGTTAGAGCCAAATATATTGGCACCCAAATCCCTCCCCCTTGTGCTTAG

mRNA sequence

ATGGCGCGCAAGGCACACGATCTCCCTGCAGTTCTTTCAAGTAACGCCCAATCTCAACCTCCTCGCCGGTCTGGCGGTCCGACGAGCTTCCGTTGCCGGAGCGATGGCCGATTTATTTCTCTTTTCCATTCCTGCGACGTTAACAATGGCGAATTCAGAATCGCCTCCATGTTCTCAGCATCTCCGATTGCGAATTTAAAGCCAAATACTCTTCCATTCACGAATCGCTCAGTTCTTAGTAGAGGAATATTGGAAGCAGACATTTCAGGAAGCTTATTGCGTTACATAGCTAAGAGTTCTCATGTTTTCAGTGTTAGAAGCTTTGGTGCTCGAGCAAATGGCTTAACTGATGATAGCAATGCCTTCTCAGAAACATGGAGGATGGCCTGTGGTTCAGCTGGAGTGGTAACTTTCCTGATTCCAAAAGGAACCTATTTGGTTGGTCCAGTTAAATTTTCAGGGCCTTGCAAAAATGTGTCGTCTCTAACGGTTAAAATGAAGGGATATTTGAAGGCATCAACAGACCTGTCAAAGTATGGTTCTGGTGCAGGCTGGGTTGAGTTCAGATGGATGGAGGGGCTAACGTTGACTGGAGGTGGAACCTTCGATGGCCGAGGCGCTGAGGCATGGCCATTCAACAACTGCCTTGATGACCACAATTGCAATCTCCTTCCTACTAACTTGAAATTCGTGGCTATGAATAACACGGTTGTTCGACGCATAACTTCGCTAAACAGCAAGTTCTTTCACTTGGCTTTAGTACAATGCAAGGGGTTCAGAGGTAGCAATCTCAAGATTTCAGCTCCAGCAAATAGTCCCAACACGGATGGTATCCACATCGACCGAAGCTCTAACATCCATCTTTCTCGATCACATATTCAAACTGGCGACGACTGCATATCCATTGGACAAGGGAACTCTCAAGTCACAATCACCGGCATCGATTGTGGACCGGGTCATGGGATCAGTGTTGGAAGCTTGGGAAGATATCAAAATGAAGGAGATGTAAGCGGATTAGTAGTTAAAAATTGTAGCCTCACAGGTACCACCAATGGGATCAGAATCAAGACATGGCCAAATTCTCCTGGCAGCAGCTCAGCCACAAACATGACATTTAAGAACTTCGCCATGAACAATGTGTCCAATCCAATTATCATTGATCAATCGTACTGTCCATTCAGATCTTGTTCTTTTAAGGCACCATCTCTAGTGAAGCTGAGTGATATTTACTTCAAGAACATAAAAGGAACCTCTTCCTCTAAGGTGGCTGTGGCATTAGAATGCAGCAAGGGATTCCCATGTCAGAATGTGAACCTTGAAAATGTTCACTTGCTTCTTTCATCAGGTCAAAATCACCCTTCTTCTACTTGCAAATATGTTAGAGCCAAATATATTGGCACCCAAATCCCTCCCCCTTGTGCTTAG

Coding sequence (CDS)

ATGGCGCGCAAGGCACACGATCTCCCTGCAGTTCTTTCAAGTAACGCCCAATCTCAACCTCCTCGCCGGTCTGGCGGTCCGACGAGCTTCCGTTGCCGGAGCGATGGCCGATTTATTTCTCTTTTCCATTCCTGCGACGTTAACAATGGCGAATTCAGAATCGCCTCCATGTTCTCAGCATCTCCGATTGCGAATTTAAAGCCAAATACTCTTCCATTCACGAATCGCTCAGTTCTTAGTAGAGGAATATTGGAAGCAGACATTTCAGGAAGCTTATTGCGTTACATAGCTAAGAGTTCTCATGTTTTCAGTGTTAGAAGCTTTGGTGCTCGAGCAAATGGCTTAACTGATGATAGCAATGCCTTCTCAGAAACATGGAGGATGGCCTGTGGTTCAGCTGGAGTGGTAACTTTCCTGATTCCAAAAGGAACCTATTTGGTTGGTCCAGTTAAATTTTCAGGGCCTTGCAAAAATGTGTCGTCTCTAACGGTTAAAATGAAGGGATATTTGAAGGCATCAACAGACCTGTCAAAGTATGGTTCTGGTGCAGGCTGGGTTGAGTTCAGATGGATGGAGGGGCTAACGTTGACTGGAGGTGGAACCTTCGATGGCCGAGGCGCTGAGGCATGGCCATTCAACAACTGCCTTGATGACCACAATTGCAATCTCCTTCCTACTAACTTGAAATTCGTGGCTATGAATAACACGGTTGTTCGACGCATAACTTCGCTAAACAGCAAGTTCTTTCACTTGGCTTTAGTACAATGCAAGGGGTTCAGAGGTAGCAATCTCAAGATTTCAGCTCCAGCAAATAGTCCCAACACGGATGGTATCCACATCGACCGAAGCTCTAACATCCATCTTTCTCGATCACATATTCAAACTGGCGACGACTGCATATCCATTGGACAAGGGAACTCTCAAGTCACAATCACCGGCATCGATTGTGGACCGGGTCATGGGATCAGTGTTGGAAGCTTGGGAAGATATCAAAATGAAGGAGATGTAAGCGGATTAGTAGTTAAAAATTGTAGCCTCACAGGTACCACCAATGGGATCAGAATCAAGACATGGCCAAATTCTCCTGGCAGCAGCTCAGCCACAAACATGACATTTAAGAACTTCGCCATGAACAATGTGTCCAATCCAATTATCATTGATCAATCGTACTGTCCATTCAGATCTTGTTCTTTTAAGGCACCATCTCTAGTGAAGCTGAGTGATATTTACTTCAAGAACATAAAAGGAACCTCTTCCTCTAAGGTGGCTGTGGCATTAGAATGCAGCAAGGGATTCCCATGTCAGAATGTGAACCTTGAAAATGTTCACTTGCTTCTTTCATCAGGTCAAAATCACCCTTCTTCTACTTGCAAATATGTTAGAGCCAAATATATTGGCACCCAAATCCCTCCCCCTTGTGCTTAG

Protein sequence

MARKAHDLPAVLSSNAQSQPPRRSGGPTSFRCRSDGRFISLFHSCDVNNGEFRIASMFSASPIANLKPNTLPFTNRSVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA
BLAST of Carg04109 vs. NCBI nr
Match: XP_022952676.1 (exopolygalacturonase isoform X2 [Cucurbita moschata])

HSP 1 Score: 801.2 bits (2068), Expect = 1.9e-228
Identity = 393/398 (98.74%), Postives = 396/398 (99.50%), Query Frame = 0

Query: 77  SVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVV 136
           SVLSRGILEADISGSLLRY AKSS VFSVRSFGARANGLTDDSNAFS+TWRMACGSAGVV
Sbjct: 20  SVLSRGILEADISGSLLRYRAKSSRVFSVRSFGARANGLTDDSNAFSQTWRMACGSAGVV 79

Query: 137 TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTL 196
           TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGW+EFRW+EGLTL
Sbjct: 80  TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWIEFRWVEGLTL 139

Query: 197 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 256
           TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC
Sbjct: 140 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 199

Query: 257 KGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 316
           KGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC
Sbjct: 200 KGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 259

Query: 317 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 376
           GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA
Sbjct: 260 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 319

Query: 377 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 436
           MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN
Sbjct: 320 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 379

Query: 437 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA
Sbjct: 380 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 417

BLAST of Carg04109 vs. NCBI nr
Match: XP_023554538.1 (exopolygalacturonase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800.8 bits (2067), Expect = 2.5e-228
Identity = 394/398 (98.99%), Postives = 395/398 (99.25%), Query Frame = 0

Query: 77  SVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVV 136
           SVLSRGILEADISGSLLRY AKSS VFSVRSFGARANGLTDDSNAFSETWRMACGSAGVV
Sbjct: 20  SVLSRGILEADISGSLLRYRAKSSRVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVV 79

Query: 137 TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTL 196
           TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGW+EFRWMEGLTL
Sbjct: 80  TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWIEFRWMEGLTL 139

Query: 197 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 256
           TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC
Sbjct: 140 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 199

Query: 257 KGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 316
           K FRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC
Sbjct: 200 KRFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 259

Query: 317 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 376
           GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA
Sbjct: 260 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 319

Query: 377 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 436
           MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN
Sbjct: 320 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 379

Query: 437 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA
Sbjct: 380 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 417

BLAST of Carg04109 vs. NCBI nr
Match: XP_022969159.1 (exopolygalacturonase isoform X2 [Cucurbita maxima])

HSP 1 Score: 795.8 bits (2054), Expect = 8.0e-227
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 77  SVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVV 136
           SVLSRGILEADISGSLLRY AKSSHVFSVRSFGARANGLTDDS+AF+ETWRMAC SAGVV
Sbjct: 20  SVLSRGILEADISGSLLRYGAKSSHVFSVRSFGARANGLTDDSDAFTETWRMACASAGVV 79

Query: 137 TFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTL 196
           TFLIPKGTYLVGPVKFSGPCKNVSSL VKMKGYLKASTDLSKYGSGAGW+EFRWMEGLTL
Sbjct: 80  TFLIPKGTYLVGPVKFSGPCKNVSSLMVKMKGYLKASTDLSKYGSGAGWIEFRWMEGLTL 139

Query: 197 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 256
           TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC
Sbjct: 140 TGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQC 199

Query: 257 KGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 316
           K FRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC
Sbjct: 200 KRFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDC 259

Query: 317 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 376
           GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA
Sbjct: 260 GPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFA 319

Query: 377 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 436
           MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN
Sbjct: 320 MNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQN 379

Query: 437 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA
Sbjct: 380 VNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 417

BLAST of Carg04109 vs. NCBI nr
Match: XP_008451195.1 (PREDICTED: exopolygalacturonase isoform X1 [Cucumis melo])

HSP 1 Score: 739.6 bits (1908), Expect = 6.8e-210
Identity = 359/400 (89.75%), Postives = 377/400 (94.25%), Query Frame = 0

Query: 75  NRSVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAG 134
           NRS LSRGILE+D+SGSLLRY  KSSHV+SVRSFGARANGLTDDSNAF   WR AC SAG
Sbjct: 2   NRSFLSRGILESDVSGSLLRYRTKSSHVYSVRSFGARANGLTDDSNAFLAAWRTACDSAG 61

Query: 135 VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGL 194
           VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKAST+LSKYGSGAGW+EFRWMEGL
Sbjct: 62  VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTNLSKYGSGAGWIEFRWMEGL 121

Query: 195 TLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALV 254
           TLTGGGTFDGRG+EAWPFNNC DD+NC LLPTNLKFVA+NNTVV+ ITSLNSKFFHLALV
Sbjct: 122 TLTGGGTFDGRGSEAWPFNNCRDDYNCKLLPTNLKFVALNNTVVQHITSLNSKFFHLALV 181

Query: 255 QCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGI 314
           QCKGF GS LKISAP NSPNTDGIHIDRSSN+H S SHIQTGDDCISIGQGNS+VTI+ I
Sbjct: 182 QCKGFIGSKLKISAPENSPNTDGIHIDRSSNVHFSLSHIQTGDDCISIGQGNSEVTISSI 241

Query: 315 DCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKN 374
           +CGPGHGISVGSLGRY+NEGDVSGLVVKNCSLTGT NGIRIKTWPNSPGSSSATNMTF+N
Sbjct: 242 NCGPGHGISVGSLGRYRNEGDVSGLVVKNCSLTGTANGIRIKTWPNSPGSSSATNMTFEN 301

Query: 375 FAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPC 434
             MNNVSNPIIIDQSYCPF  CSFKAPSLVKLSDIYFKNI+GTSSSKVAVALECSKGFPC
Sbjct: 302 ITMNNVSNPIIIDQSYCPFGFCSFKAPSLVKLSDIYFKNIRGTSSSKVAVALECSKGFPC 361

Query: 435 QNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           QNVNLENVHL LSSG+NHPSSTCKYVRA+YIGTQIPPPCA
Sbjct: 362 QNVNLENVHLDLSSGENHPSSTCKYVRARYIGTQIPPPCA 401

BLAST of Carg04109 vs. NCBI nr
Match: KGN43365.1 (hypothetical protein Csa_7G027810 [Cucumis sativus])

HSP 1 Score: 728.0 bits (1878), Expect = 2.0e-206
Identity = 374/477 (78.41%), Postives = 396/477 (83.02%), Query Frame = 0

Query: 1   MARKAHDL-PAVLSSNAQSQPP-RRSGGPTSFRCRSDGRFISLFHSCDVNNGEFRIASMF 60
           M    HDL P  L++NA S     RSGGP+ F              C   +G        
Sbjct: 1   MTLNPHDLSPFFLTTNALSTSALLRSGGPSRF--------------CSTGSG-------- 60

Query: 61  SASPIANLKPNTLPFTNRSVLSRGILEADISGSLLRYIAK-SSHVFSVRSFGARANGLTD 120
                           + SV+SRGILE+D+SGSLLRY  K SSHVFSVRSFGARANGLTD
Sbjct: 61  ----------------SSSVISRGILESDVSGSLLRYRTKSSSHVFSVRSFGARANGLTD 120

Query: 121 DSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLS 180
           DSNAF   WRMAC SAGVVTFLIPKGTYLVGPV FSGPCKNVSSLTVKM+GYLKAST+LS
Sbjct: 121 DSNAFLAAWRMACDSAGVVTFLIPKGTYLVGPVIFSGPCKNVSSLTVKMEGYLKASTNLS 180

Query: 181 KYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTV 240
           KYGSGAGW+EFRWMEGLTL GGGTFDGRGAEAWPFNNC DD+NC LLPTNLKFVA+NNTV
Sbjct: 181 KYGSGAGWIEFRWMEGLTLNGGGTFDGRGAEAWPFNNCRDDYNCKLLPTNLKFVALNNTV 240

Query: 241 VRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGD 300
           V+ ITSLNSKFFHLALVQCK F GS LKISAP NSPNTDGIHIDRSSNIH S SHIQTGD
Sbjct: 241 VQHITSLNSKFFHLALVQCKRFVGSELKISAPENSPNTDGIHIDRSSNIHFSLSHIQTGD 300

Query: 301 DCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKT 360
           DCISIGQGNSQVTI+ ++CGPGHGISVGSLGRY+NEGDVSGLVVKNCSLTGT NGIRIKT
Sbjct: 301 DCISIGQGNSQVTISSVNCGPGHGISVGSLGRYRNEGDVSGLVVKNCSLTGTANGIRIKT 360

Query: 361 WPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGT 420
           WPNSPGSSSATNMTF+N  MNNVSNPIIIDQSYCPF  CSFKAPSLVKLSDIYFKNI+GT
Sbjct: 361 WPNSPGSSSATNMTFENITMNNVSNPIIIDQSYCPFGFCSFKAPSLVKLSDIYFKNIRGT 420

Query: 421 SSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           SSSKVAVALECSKGFPCQNVNLENVHL LSSG+NHPSSTCKYVRA+YIGTQIPPPCA
Sbjct: 421 SSSKVAVALECSKGFPCQNVNLENVHLDLSSGENHPSSTCKYVRARYIGTQIPPPCA 439

BLAST of Carg04109 vs. TAIR10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 525.8 bits (1353), Expect = 2.8e-149
Identity = 262/392 (66.84%), Postives = 306/392 (78.06%), Query Frame = 0

Query: 96  IAKSSHVF--SVRSFGARANGLTDDSNAFSETWRMAC-GSAGVVTFLIPKGTYLVGPVKF 155
           I   S VF   VRSFGARAN   D + AF   W  AC  S+  V  +IP+G + VG ++F
Sbjct: 26  IVDKSSVFLVDVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRF 85

Query: 156 SGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPF 215
           SGPC NVS+LTV+    +KASTDLSKY SG GW++F W+ GLTLTGGGTFDG+GA AWPF
Sbjct: 86  SGPCTNVSNLTVR----VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPF 145

Query: 216 NNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANS 275
           NNC  D NC LLPT+LKFV MN TVVRRI+S+NSKFFH+ALV+C+ F+G+ L I+AP++S
Sbjct: 146 NNCTSDSNCKLLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDS 205

Query: 276 PNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQN 335
           PNTDGIHI+RSSN++ SRSHI TGDDC+SIGQGNSQ+TIT I CGPGHGISVGSLGRY N
Sbjct: 206 PNTDGIHIERSSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPN 265

Query: 336 EGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCP 395
           E DV+GLVVK+C ++GTTNGIRIKTW NSPG S+ATNMTF+N  MNNV+NPIIIDQSYCP
Sbjct: 266 EKDVNGLVVKDCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCP 325

Query: 396 FRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSS---- 455
           F SC    PS V+LS+IYFKNI+GTSSS VAV L CS+G PC+ V LENVHL LSS    
Sbjct: 326 FSSCISNVPSKVELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGG 385

Query: 456 -------GQNHPSSTCKYVRAKYIGTQIPPPC 474
                  G    SS+C+ VRA YIGTQIPPPC
Sbjct: 386 RKQSSNRGNEAVSSSCRNVRANYIGTQIPPPC 413

BLAST of Carg04109 vs. TAIR10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 329.7 bits (844), Expect = 2.9e-90
Identity = 160/359 (44.57%), Postives = 225/359 (62.67%), Query Frame = 0

Query: 116 TDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTD 175
           TDDS AF+  W+ AC +   +T  +PKG YLV  ++F GPCK    +T+++ G  KA   
Sbjct: 84  TDDSAAFAAAWKEACAAGSTIT--VPKGEYLVESLEFKGPCK--GPVTLELNGNFKAPAT 143

Query: 176 LSKYGSGAGWVEFRWMEGLTLTGG-GTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMN 235
           +      AGW++F  +   TL G    FDG+G+ AW  N+C     CN LP N++F  + 
Sbjct: 144 VKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLT 203

Query: 236 NTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQ 295
           N+ +  ITS NSK FH+ ++ CK    +++ I AP  S NTDGIHI RS+ ++L  + I+
Sbjct: 204 NSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIK 263

Query: 296 TGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIR 355
           TGDDC+SIG G   + +  ++CGPGHGIS+GSLGRY NE  V G+ V+ C +  T NG+R
Sbjct: 264 TGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVR 323

Query: 356 IKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNI 415
           IKTWP SP    A+N+ F++  M+NVS P++IDQ YCP+  C    PS VKLSD+  KNI
Sbjct: 324 IKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNI 383

Query: 416 KGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPC 474
           KGTS++KVAV L CSKG PC N+ L +++L+ +  +    S C  ++    G  +P  C
Sbjct: 384 KGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAAC 437

BLAST of Carg04109 vs. TAIR10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 329.3 bits (843), Expect = 3.8e-90
Identity = 160/365 (43.84%), Postives = 227/365 (62.19%), Query Frame = 0

Query: 110 ARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGY 169
           A+ +  TDDS AF+  W+ AC +   +T  +PKG Y+V  ++F GPCK    +T+++ G 
Sbjct: 77  AKGDSKTDDSAAFAAAWKEACAAGSTIT--VPKGEYMVESLEFKGPCK--GPVTLELNGN 136

Query: 170 LKASTDLSKYGSGAGWVEFRWMEGLTLTGG-GTFDGRGAEAWPFNNCLDDHNCNLLPTNL 229
            KA   +      AGW++F  +   TL G    FDG+G+ AW  N+C     CN LP N+
Sbjct: 137 FKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINI 196

Query: 230 KFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHL 289
           +F  + N+ +  ITS NSK FH+ ++ CK    S++ I AP  S NTDGIHI RS+ ++L
Sbjct: 197 RFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNL 256

Query: 290 SRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTG 349
             + I+TGDDC+SIG G   + +  ++CGPGHGIS+GSLGRY NE  V G+ V+ C +  
Sbjct: 257 IGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKN 316

Query: 350 TTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSD 409
           T NG+RIKTWP SP    A+N+ F++  M+NVS P++IDQ YCP+  C    PS VKLSD
Sbjct: 317 TDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSD 376

Query: 410 IYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQ 469
           +  K IKGTS++KVAV L CSKG PC N+ L +++L+ +  +    S C  ++    G  
Sbjct: 377 VTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKL 436

Query: 470 IPPPC 474
           +P  C
Sbjct: 437 VPAAC 436

BLAST of Carg04109 vs. TAIR10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-87
Identity = 157/359 (43.73%), Postives = 227/359 (63.23%), Query Frame = 0

Query: 116 TDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTD 175
           +D +NA  + +  AC      T +IPKG Y +G +   GPCK  + + + + G +KA  +
Sbjct: 34  SDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCK--APIRIALLGTVKADGN 93

Query: 176 LSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNN 235
            +       WV FR + G  L GGG FDG G  AW  NNC    NC  LP +++F  + +
Sbjct: 94  AN---GKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTD 153

Query: 236 TVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQT 295
             +R ITSL++K FH+ ++  K     ++KI APA SPNTDGIH+ RS  I +  S I T
Sbjct: 154 AKIRGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFIST 213

Query: 296 GDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRI 355
           GDDC+S+G G   + +  + CGPGHGIS+GSLGRY +E +VSG+ + NC+L  T NG+RI
Sbjct: 214 GDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRI 273

Query: 356 KTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIK 415
           KTWP++  +++A+++ F+N  + NVSNPI+IDQ YCP+  C+ + PS +KL++I FK I+
Sbjct: 274 KTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIR 333

Query: 416 GTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           GTS +K AV L CSKG+PCQNV + +V++  +      +  C  V  K +GTQIP  C+
Sbjct: 334 GTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACS 387

BLAST of Carg04109 vs. TAIR10
Match: AT1G78400.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-86
Identity = 163/371 (43.94%), Postives = 236/371 (63.61%), Query Frame = 0

Query: 102 VFSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSS 161
           VF VRS+GAR +G TD++ AF++ W+ AC   G+    IP GT+ +G V F+GPCK  S 
Sbjct: 34  VFDVRSYGARGDGKTDNTMAFTKAWKDACQWKGLPRVYIPFGTFYLGAVAFTGPCK--SR 93

Query: 162 LTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNC 221
           ++  +KG L A  D +     + W+ FR+++ LT++GGG  DG+G+ +WP NNC   HNC
Sbjct: 94  ISFIIKGTLLAPKDPNAIKQDS-WIIFRYVDYLTVSGGGILDGQGSYSWPLNNCRQTHNC 153

Query: 222 NLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHID 281
             LP N+ F  +  + + RI S+NSK  HL     + F  + + I AP +SPNTDGI I 
Sbjct: 154 RALPMNMGFQFVRFSRLTRIKSINSKMGHLNFFSVQHFDITRVNIKAPGDSPNTDGIKIG 213

Query: 282 RSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVV 341
            S+++ +    I TGDDCI+I  G   + I  ++CGPGHGISVGSLG+++ E  V GL+V
Sbjct: 214 SSNHMKIHHVDIATGDDCIAILSGTFNLDINKVNCGPGHGISVGSLGKFKGEKSVQGLIV 273

Query: 342 KNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAP 401
           +N    GT+NG+RIKTWP+    +  +N  FKN  M +V +PI IDQ YCP   CSF++ 
Sbjct: 274 RNSIFNGTSNGVRIKTWPSPGEPNLVSNFLFKNLQMIDVQSPINIDQRYCPNPPCSFQSF 333

Query: 402 SLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVR 461
           S +++ D+ F+NI GTS++K AV L+CSK  PC+NV L N++++        +S C+ V 
Sbjct: 334 SKIQIRDVKFQNIWGTSTAKEAVKLQCSKNVPCKNVQLFNINIVHRGRDGPATSVCENV- 393

Query: 462 AKYIGTQIPPP 473
             +IG +I PP
Sbjct: 394 GGWIGGKISPP 400

BLAST of Carg04109 vs. Swiss-Prot
Match: sp|Q6H9K0|PGLR2_PLAAC (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.5e-99
Identity = 179/377 (47.48%), Postives = 247/377 (65.52%), Query Frame = 0

Query: 94  RYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFS 153
           R +  S  VF+V  +GA+  G  D S A  + W+ AC S G  T LIPKG Y +G V   
Sbjct: 1   RGVQSSGSVFNVNDYGAKGAG--DISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQ 60

Query: 154 GPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFN 213
           GPCK  S +  ++ G +KA  D SK+ S  GWV F  ++GLT++G GT DG+G  AW  N
Sbjct: 61  GPCKG-SKIGFQIDGVVKAPADPSKFKSD-GWVSFYRIDGLTVSGTGTLDGQGQTAWAKN 120

Query: 214 NCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSP 273
           NC  + NC     NL+F  + + +VR ITSLNSK FH+ +++C+     ++ ++AP  S 
Sbjct: 121 NCDKNPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSI 180

Query: 274 NTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNE 333
           NTDGIH+  S  + ++ + I TGDDCISIG G+  VTIT ++CGPGHGIS+GSLGRY NE
Sbjct: 181 NTDGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNE 240

Query: 334 GDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPF 393
            +V G+ VK C+ +GT NG+R+KTWPNSP   +AT++TF++  MNNV NP+I+DQ YCP+
Sbjct: 241 KEVRGITVKGCTFSGTMNGVRVKTWPNSP-PGAATDLTFQDLTMNNVQNPVILDQEYCPY 300

Query: 394 RSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHP 453
             CS +APS +KLS+I F NI+GTS+ KVAV + CS G PC N+ +  ++L         
Sbjct: 301 GQCSRQAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPA 360

Query: 454 SSTCKYVRAKYIGTQIP 471
           +STC  V+  + G Q+P
Sbjct: 361 TSTCSNVKPTFSGKQVP 372

BLAST of Carg04109 vs. Swiss-Prot
Match: sp|P24548|PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 6.7e-92
Identity = 172/360 (47.78%), Postives = 232/360 (64.44%), Query Frame = 0

Query: 117 DDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDL 176
           D + A +  W+ AC SA   T L+PKG + VG +   GPCK  SS+ ++++G LKA  D 
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCK--SSIGLQLQGTLKAPADP 60

Query: 177 SKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNC-LDDHNCNLLPTNLKFVAMNN 236
           SK   G GW+    ++ LT+ GGG FDG+G  AW  N+C  +   C  L  NL+  A+ N
Sbjct: 61  SKI-KGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTN 120

Query: 237 TVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQT 296
           +++R +T+L+SK FH+ ++ CK       KISA   S NTDGIHI RS  +++  + I+T
Sbjct: 121 SILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKT 180

Query: 297 GDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRI 356
           GDDCIS+G G+  + IT I CGPGHGISVGSLGRY+NE  V G+ VKNC++TG+ NG+RI
Sbjct: 181 GDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRI 240

Query: 357 KTWPNS-PGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNI 416
           KTWP S PG   A+ M F++  MN+V  PI+IDQ YCP+  C+ + PS VKLS I FKNI
Sbjct: 241 KTWPKSEPG--EASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNI 300

Query: 417 KGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           KGTS++K AV L CSK FPC  V L ++ L  S      +S C+ ++    G QIP  C+
Sbjct: 301 KGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICS 355

BLAST of Carg04109 vs. Swiss-Prot
Match: sp|P49063|PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 329.3 bits (843), Expect = 6.9e-89
Identity = 160/365 (43.84%), Postives = 227/365 (62.19%), Query Frame = 0

Query: 110 ARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGY 169
           A+ +  TDDS AF+  W+ AC +   +T  +PKG Y+V  ++F GPCK    +T+++ G 
Sbjct: 77  AKGDSKTDDSAAFAAAWKEACAAGSTIT--VPKGEYMVESLEFKGPCK--GPVTLELNGN 136

Query: 170 LKASTDLSKYGSGAGWVEFRWMEGLTLTGG-GTFDGRGAEAWPFNNCLDDHNCNLLPTNL 229
            KA   +      AGW++F  +   TL G    FDG+G+ AW  N+C     CN LP N+
Sbjct: 137 FKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINI 196

Query: 230 KFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHL 289
           +F  + N+ +  ITS NSK FH+ ++ CK    S++ I AP  S NTDGIHI RS+ ++L
Sbjct: 197 RFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNL 256

Query: 290 SRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTG 349
             + I+TGDDC+SIG G   + +  ++CGPGHGIS+GSLGRY NE  V G+ V+ C +  
Sbjct: 257 IGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKN 316

Query: 350 TTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSD 409
           T NG+RIKTWP SP    A+N+ F++  M+NVS P++IDQ YCP+  C    PS VKLSD
Sbjct: 317 TDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSD 376

Query: 410 IYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQ 469
           +  K IKGTS++KVAV L CSKG PC N+ L +++L+ +  +    S C  ++    G  
Sbjct: 377 VTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKL 436

Query: 470 IPPPC 474
           +P  C
Sbjct: 437 VPAAC 436

BLAST of Carg04109 vs. Swiss-Prot
Match: sp|P35339|PGLR3_MAIZE (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.7e-87
Identity = 158/364 (43.41%), Postives = 226/364 (62.09%), Query Frame = 0

Query: 103 FSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSL 162
           F +   GA  NG TD + A  E W  ACG  G  T LIPKG +LVGP+ F+GPCK    +
Sbjct: 41  FDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCK--GDV 100

Query: 163 TVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCN 222
           T+++ G L A+TDLS+Y     W+E   ++ L +TG G  DG+G   W  N+C+  ++C 
Sbjct: 101 TIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCK 160

Query: 223 LLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDR 282
           +LP +L    +NN  V  IT LNSKFFH+ + +CK     ++ ++AP +SPNTDGIH+  
Sbjct: 161 ILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGD 220

Query: 283 SSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVK 342
           SS + ++ + I  GDDCISIG G S+V ITG+ CGPGHGIS+GSLGRY++E DV+ + VK
Sbjct: 221 SSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVK 280

Query: 343 NCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPS 402
           +C+L  T NG+RIK + ++    +A+ + ++N  M +   PIIID  YCP + C+    S
Sbjct: 281 DCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGAS 340

Query: 403 LVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRA 462
            V + D+ FKNI GTSS+  AV L C+   PC  V +++V++  S   N   + CK  + 
Sbjct: 341 KVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKG 400

Query: 463 KYIG 467
              G
Sbjct: 401 SAKG 402

BLAST of Carg04109 vs. Swiss-Prot
Match: sp|Q39766|PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.0e-84
Identity = 163/370 (44.05%), Postives = 227/370 (61.35%), Query Frame = 0

Query: 105 VRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTV 164
           V  FGA+A+G TD S  F + W+ AC S    T +IPKGTYL+  V   GPCK  + + +
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCK--APIEI 91

Query: 165 KMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLL 224
            ++G ++A  D S +     WV F  +E   + GGG FDG+G+ A+  N C +    + L
Sbjct: 92  NVQGTIQAPADPSAF-KDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKL 151

Query: 225 PTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSS 284
           P N++F  + N +++ ITS +SK FH+ +  CK      LKI AP  SPNTDGIH+ +S 
Sbjct: 152 PVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSE 211

Query: 285 NIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNC 344
            +++  S I+TGDDCISIG G   + I  I CGPGHGIS+GSLG++QNE  V G+ + NC
Sbjct: 212 GVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNC 271

Query: 345 SLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLV 404
           ++T T+NG RIKTWP   G  + + + F++  MNNVS+PI+IDQ YCP+  C     S V
Sbjct: 272 TITNTSNGARIKTWPGEHG-GAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKV 331

Query: 405 KLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKY 464
           KLS+I FKNI+GTS+   A+   CS   PCQNV L ++  +  +G    +S C  V+   
Sbjct: 332 KLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADID-IQHNGAEPATSQCLNVKPIT 391

Query: 465 IGTQIPPPCA 475
           IG   P PC+
Sbjct: 392 IGKLNPIPCS 396

BLAST of Carg04109 vs. TrEMBL
Match: tr|A0A1S3BQX3|A0A1S3BQX3_CUCME (exopolygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492566 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 4.5e-210
Identity = 359/400 (89.75%), Postives = 377/400 (94.25%), Query Frame = 0

Query: 75  NRSVLSRGILEADISGSLLRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAG 134
           NRS LSRGILE+D+SGSLLRY  KSSHV+SVRSFGARANGLTDDSNAF   WR AC SAG
Sbjct: 2   NRSFLSRGILESDVSGSLLRYRTKSSHVYSVRSFGARANGLTDDSNAFLAAWRTACDSAG 61

Query: 135 VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGL 194
           VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKAST+LSKYGSGAGW+EFRWMEGL
Sbjct: 62  VVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTNLSKYGSGAGWIEFRWMEGL 121

Query: 195 TLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALV 254
           TLTGGGTFDGRG+EAWPFNNC DD+NC LLPTNLKFVA+NNTVV+ ITSLNSKFFHLALV
Sbjct: 122 TLTGGGTFDGRGSEAWPFNNCRDDYNCKLLPTNLKFVALNNTVVQHITSLNSKFFHLALV 181

Query: 255 QCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGI 314
           QCKGF GS LKISAP NSPNTDGIHIDRSSN+H S SHIQTGDDCISIGQGNS+VTI+ I
Sbjct: 182 QCKGFIGSKLKISAPENSPNTDGIHIDRSSNVHFSLSHIQTGDDCISIGQGNSEVTISSI 241

Query: 315 DCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKN 374
           +CGPGHGISVGSLGRY+NEGDVSGLVVKNCSLTGT NGIRIKTWPNSPGSSSATNMTF+N
Sbjct: 242 NCGPGHGISVGSLGRYRNEGDVSGLVVKNCSLTGTANGIRIKTWPNSPGSSSATNMTFEN 301

Query: 375 FAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPC 434
             MNNVSNPIIIDQSYCPF  CSFKAPSLVKLSDIYFKNI+GTSSSKVAVALECSKGFPC
Sbjct: 302 ITMNNVSNPIIIDQSYCPFGFCSFKAPSLVKLSDIYFKNIRGTSSSKVAVALECSKGFPC 361

Query: 435 QNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           QNVNLENVHL LSSG+NHPSSTCKYVRA+YIGTQIPPPCA
Sbjct: 362 QNVNLENVHLDLSSGENHPSSTCKYVRARYIGTQIPPPCA 401

BLAST of Carg04109 vs. TrEMBL
Match: tr|A0A0A0K0Z1|A0A0A0K0Z1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G027810 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 1.3e-206
Identity = 374/477 (78.41%), Postives = 396/477 (83.02%), Query Frame = 0

Query: 1   MARKAHDL-PAVLSSNAQSQPP-RRSGGPTSFRCRSDGRFISLFHSCDVNNGEFRIASMF 60
           M    HDL P  L++NA S     RSGGP+ F              C   +G        
Sbjct: 1   MTLNPHDLSPFFLTTNALSTSALLRSGGPSRF--------------CSTGSG-------- 60

Query: 61  SASPIANLKPNTLPFTNRSVLSRGILEADISGSLLRYIAK-SSHVFSVRSFGARANGLTD 120
                           + SV+SRGILE+D+SGSLLRY  K SSHVFSVRSFGARANGLTD
Sbjct: 61  ----------------SSSVISRGILESDVSGSLLRYRTKSSSHVFSVRSFGARANGLTD 120

Query: 121 DSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTVKMKGYLKASTDLS 180
           DSNAF   WRMAC SAGVVTFLIPKGTYLVGPV FSGPCKNVSSLTVKM+GYLKAST+LS
Sbjct: 121 DSNAFLAAWRMACDSAGVVTFLIPKGTYLVGPVIFSGPCKNVSSLTVKMEGYLKASTNLS 180

Query: 181 KYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLLPTNLKFVAMNNTV 240
           KYGSGAGW+EFRWMEGLTL GGGTFDGRGAEAWPFNNC DD+NC LLPTNLKFVA+NNTV
Sbjct: 181 KYGSGAGWIEFRWMEGLTLNGGGTFDGRGAEAWPFNNCRDDYNCKLLPTNLKFVALNNTV 240

Query: 241 VRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSSNIHLSRSHIQTGD 300
           V+ ITSLNSKFFHLALVQCK F GS LKISAP NSPNTDGIHIDRSSNIH S SHIQTGD
Sbjct: 241 VQHITSLNSKFFHLALVQCKRFVGSELKISAPENSPNTDGIHIDRSSNIHFSLSHIQTGD 300

Query: 301 DCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNCSLTGTTNGIRIKT 360
           DCISIGQGNSQVTI+ ++CGPGHGISVGSLGRY+NEGDVSGLVVKNCSLTGT NGIRIKT
Sbjct: 301 DCISIGQGNSQVTISSVNCGPGHGISVGSLGRYRNEGDVSGLVVKNCSLTGTANGIRIKT 360

Query: 361 WPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLVKLSDIYFKNIKGT 420
           WPNSPGSSSATNMTF+N  MNNVSNPIIIDQSYCPF  CSFKAPSLVKLSDIYFKNI+GT
Sbjct: 361 WPNSPGSSSATNMTFENITMNNVSNPIIIDQSYCPFGFCSFKAPSLVKLSDIYFKNIRGT 420

Query: 421 SSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKYIGTQIPPPCA 475
           SSSKVAVALECSKGFPCQNVNLENVHL LSSG+NHPSSTCKYVRA+YIGTQIPPPCA
Sbjct: 421 SSSKVAVALECSKGFPCQNVNLENVHLDLSSGENHPSSTCKYVRARYIGTQIPPPCA 439

BLAST of Carg04109 vs. TrEMBL
Match: tr|A0A1S3BRP5|A0A1S3BRP5_CUCME (exopolygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492566 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 2.2e-196
Identity = 335/370 (90.54%), Postives = 350/370 (94.59%), Query Frame = 0

Query: 105 VRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTV 164
           VRSFGARANGLTDDSNAF   WR AC SAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTV
Sbjct: 4   VRSFGARANGLTDDSNAFLAAWRTACDSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSLTV 63

Query: 165 KMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCNLL 224
           KMKGYLKAST+LSKYGSGAGW+EFRWMEGLTLTGGGTFDGRG+EAWPFNNC DD+NC LL
Sbjct: 64  KMKGYLKASTNLSKYGSGAGWIEFRWMEGLTLTGGGTFDGRGSEAWPFNNCRDDYNCKLL 123

Query: 225 PTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDRSS 284
           PTNLKFVA+NNTVV+ ITSLNSKFFHLALVQCKGF GS LKISAP NSPNTDGIHIDRSS
Sbjct: 124 PTNLKFVALNNTVVQHITSLNSKFFHLALVQCKGFIGSKLKISAPENSPNTDGIHIDRSS 183

Query: 285 NIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVKNC 344
           N+H S SHIQTGDDCISIGQGNS+VTI+ I+CGPGHGISVGSLGRY+NEGDVSGLVVKNC
Sbjct: 184 NVHFSLSHIQTGDDCISIGQGNSEVTISSINCGPGHGISVGSLGRYRNEGDVSGLVVKNC 243

Query: 345 SLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPSLV 404
           SLTGT NGIRIKTWPNSPGSSSATNMTF+N  MNNVSNPIIIDQSYCPF  CSFKAPSLV
Sbjct: 244 SLTGTANGIRIKTWPNSPGSSSATNMTFENITMNNVSNPIIIDQSYCPFGFCSFKAPSLV 303

Query: 405 KLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRAKY 464
           KLSDIYFKNI+GTSSSKVAVALECSKGFPCQNVNLENVHL LSSG+NHPSSTCKYVRA+Y
Sbjct: 304 KLSDIYFKNIRGTSSSKVAVALECSKGFPCQNVNLENVHLDLSSGENHPSSTCKYVRARY 363

Query: 465 IGTQIPPPCA 475
           IGTQIPPPCA
Sbjct: 364 IGTQIPPPCA 373

BLAST of Carg04109 vs. TrEMBL
Match: tr|A0A2P4MVT4|A0A2P4MVT4_QUESU (Exopolygalacturonase OS=Quercus suber OX=58331 GN=CFP56_26326 PE=3 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 4.2e-168
Identity = 281/372 (75.54%), Postives = 327/372 (87.90%), Query Frame = 0

Query: 103 FSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKFSGPCKNVSSL 162
           F V SFGARA+G TDD+ AF   W+ AC S+G V  LIPKGTYL+G ++F+GPC NVSSL
Sbjct: 144 FDVMSFGARADGRTDDTRAFRTAWKKACESSGSVNLLIPKGTYLIGHIEFTGPCNNVSSL 203

Query: 163 TVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPFNNCLDDHNCN 222
           TV +KGYLKA+TDLSKYG  AGWVEF W+EGLTLTGGGT DG+GA+AWP+N+C  D NC 
Sbjct: 204 TVHLKGYLKATTDLSKYGFSAGWVEFGWVEGLTLTGGGTLDGQGAKAWPYNDCPTDSNCK 263

Query: 223 LLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANSPNTDGIHIDR 282
           LLPTN+KF+AMN TVVR ITSLNSKFFH+ALV+CK F+GS +KISAP+NSPNTDGIHI+R
Sbjct: 264 LLPTNVKFMAMNKTVVRGITSLNSKFFHIALVECKNFKGSKIKISAPSNSPNTDGIHIER 323

Query: 283 SSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQNEGDVSGLVVK 342
           SS+++ S SHI TGDDCIS+GQGNSQVTITG+ CGPGHGISVGSLGRY+NEGDVSGL+V+
Sbjct: 324 SSSVYFSGSHIGTGDDCISVGQGNSQVTITGVTCGPGHGISVGSLGRYRNEGDVSGLLVR 383

Query: 343 NCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCPFRSCSFKAPS 402
           +C++TGTTNGIRIKTW NSPGSS+ TNMTF+N  MNNV+NPIIIDQ+YCPF SC+  APS
Sbjct: 384 DCTMTGTTNGIRIKTWENSPGSSAVTNMTFENIVMNNVTNPIIIDQTYCPFTSCTSMAPS 443

Query: 403 LVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNHPSSTCKYVRA 462
            VK+SDIYFKNI+GTSSSKVAVALECSKG PCQN+ LENVHL LSSG+ H +STCK VRA
Sbjct: 444 RVKISDIYFKNIRGTSSSKVAVALECSKGMPCQNIYLENVHLDLSSGEKHATSTCKNVRA 503

Query: 463 KYIGTQIPPPCA 475
           KYIGTQ+PPPC+
Sbjct: 504 KYIGTQLPPPCS 515

BLAST of Carg04109 vs. TrEMBL
Match: tr|A0A2H5P1C9|A0A2H5P1C9_CITUN (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_094870 PE=3 SV=1)

HSP 1 Score: 589.0 bits (1517), Expect = 9.8e-165
Identity = 280/382 (73.30%), Postives = 324/382 (84.82%), Query Frame = 0

Query: 93  LRYIAKSSHVFSVRSFGARANGLTDDSNAFSETWRMACGSAGVVTFLIPKGTYLVGPVKF 152
           + Y   SS + +V+ FGARA+G TDDS AF   W+ AC + G VT L+P GTYL+GP+KF
Sbjct: 36  IHYKGNSSLLVNVKDFGARADGRTDDSKAFEAAWKEACETTGAVTLLVPHGTYLIGPIKF 95

Query: 153 SGPCKNVSSLTVKMKGYLKASTDLSKYGSGAGWVEFRWMEGLTLTGGGTFDGRGAEAWPF 212
           +GPCKNVS++TV+MKGYL AST+LS Y SGAGWVEF  +EGLTLTGGGTFDGRGA+AWP+
Sbjct: 96  AGPCKNVSNITVQMKGYLIASTNLSVYRSGAGWVEFGGVEGLTLTGGGTFDGRGAKAWPY 155

Query: 213 NNCLDDHNCNLLPTNLKFVAMNNTVVRRITSLNSKFFHLALVQCKGFRGSNLKISAPANS 272
           N C    NC LLPTN+KFVAM  T+VRRITS+NSK FH+ALV+CK FRGS +KISAPANS
Sbjct: 156 NGCPTHFNCKLLPTNVKFVAMKKTIVRRITSVNSKSFHIALVECKNFRGSKIKISAPANS 215

Query: 273 PNTDGIHIDRSSNIHLSRSHIQTGDDCISIGQGNSQVTITGIDCGPGHGISVGSLGRYQN 332
           PNTDGIHI+RSS++H+SRSHI TGDDCIS+GQGNS+VTI  I CGPGHGISVGSLGRY N
Sbjct: 216 PNTDGIHIERSSSVHVSRSHIGTGDDCISVGQGNSEVTIASITCGPGHGISVGSLGRYPN 275

Query: 333 EGDVSGLVVKNCSLTGTTNGIRIKTWPNSPGSSSATNMTFKNFAMNNVSNPIIIDQSYCP 392
           EGDV GLVV++ ++TGT NG+RIKTW NSPGSS+ATNMTF+N  MNNVSNPIIIDQ+YCP
Sbjct: 276 EGDVRGLVVRDSTMTGTMNGVRIKTWANSPGSSAATNMTFENIKMNNVSNPIIIDQAYCP 335

Query: 393 FRSCSFKAPSLVKLSDIYFKNIKGTSSSKVAVALECSKGFPCQNVNLENVHLLLSSGQNH 452
           F SC  K PS VKLSDIYFKNI+GTSSS VAVALECSKG PCQN+ LENVHL LSSG+  
Sbjct: 336 FTSCPTKPPSRVKLSDIYFKNIRGTSSSAVAVALECSKGIPCQNIYLENVHLDLSSGEKQ 395

Query: 453 PSSTCKYVRAKYIGTQIPPPCA 475
           P+S+CK V AKYIGTQ+PPPCA
Sbjct: 396 PTSSCKNVEAKYIGTQVPPPCA 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022952676.11.9e-22898.74exopolygalacturonase isoform X2 [Cucurbita moschata][more]
XP_023554538.12.5e-22898.99exopolygalacturonase isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022969159.18.0e-22798.24exopolygalacturonase isoform X2 [Cucurbita maxima][more]
XP_008451195.16.8e-21089.75PREDICTED: exopolygalacturonase isoform X1 [Cucumis melo][more]
KGN43365.12.0e-20678.41hypothetical protein Csa_7G027810 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT4G18180.12.8e-14966.84Pectin lyase-like superfamily protein[more]
AT3G14040.12.9e-9044.57Pectin lyase-like superfamily protein[more]
AT3G07850.13.8e-9043.84Pectin lyase-like superfamily protein[more]
AT5G48140.11.8e-8743.73Pectin lyase-like superfamily protein[more]
AT1G78400.12.6e-8643.94Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
sp|Q6H9K0|PGLR2_PLAAC2.5e-9947.48Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
sp|P24548|PGLR_OENOR6.7e-9247.78Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
sp|P49063|PGLR2_ARATH6.9e-8943.84Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ... [more]
sp|P35339|PGLR3_MAIZE1.7e-8743.41Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
sp|Q39766|PGLR_GOSBA1.0e-8444.05Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3BQX3|A0A1S3BQX3_CUCME4.5e-21089.75exopolygalacturonase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492566 PE=3 SV=... [more]
tr|A0A0A0K0Z1|A0A0A0K0Z1_CUCSA1.3e-20678.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G027810 PE=3 SV=1[more]
tr|A0A1S3BRP5|A0A1S3BRP5_CUCME2.2e-19690.54exopolygalacturonase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492566 PE=3 SV=... [more]
tr|A0A2P4MVT4|A0A2P4MVT4_QUESU4.2e-16875.54Exopolygalacturonase OS=Quercus suber OX=58331 GN=CFP56_26326 PE=3 SV=1[more]
tr|A0A2H5P1C9|A0A2H5P1C9_CITUN9.8e-16573.30Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_094870 PE=3 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: INTERPRO
TermDefinition
IPR011050Pectin_lyase_fold/virulence
IPR000743Glyco_hydro_28
IPR012334Pectin_lyas_fold
IPR006626PbH1
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04109-RACarg04109-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 283..304
e-value: 180.0
score: 10.9
coord: 336..357
e-value: 7.5
score: 15.5
coord: 306..326
e-value: 6600.0
score: 1.4
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 92..469
e-value: 3.4E-114
score: 384.0
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 130..463
e-value: 1.5E-93
score: 313.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 313..326
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 97..474
NoneNo IPR availablePANTHERPTHR31375:SF11GLYCOSIDE HYDROLASE FAMILY 28 PROTEIN / POLYGALACTURONASE PECTINASE FAMILY PROTEINcoord: 97..474
IPR011050Pectin lyase fold/virulence factorSUPERFAMILYSSF51126Pectin lyase-likecoord: 98..473

The following gene(s) are paralogous to this gene:

None