CSPI04G20690 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G20690
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	RING/U-box superfamily protein
Location	Chr4 : 18939388 .. 18940083 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTTCTCTCTTCCTACGACATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGGTGATGATCAGTGCAGTGTTTGTTATGAACAACTGTATGGAAAAAGAGAGAATGAAAACGAAAACGAAAAGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAGAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

mRNA sequence

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTTCTCTCTTCCTACGACATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGGTGATGATCAGTGCAGTGTTTGTTATGAACAACTGTATGGAAAAAGAGAGAATGAAAACGAAAACGAAAAGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAGAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Coding sequence (CDS)

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTTCTCTCTTCCTACGACATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGGTGATGATCAGTGCAGTGTTTGTTATGAACAACTGTATGGAAAAAGAGAGAATGAAAACGAAAACGAAAAGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAGAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Homology

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BLAST of CSPI04G20690 vs. Swiss-Prot
Match: RN165_HUMAN (RING finger protein 165 OS=Homo sapiens GN=RNF165 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.9e-10
Identity = 34/96 (35.42%), Postives = 54/96 (56.25%), Query Frame = 1


Query: 146 VRKGASTSVIER-VMKEKY------DGNG----GEEKEGDDQCSVCYEQLYGKRENENEN 205
           V +GA  + IER     KY      DG G    GEE + D++C++C   L        E+
Sbjct: 252 VTRGAVQNTIERFTFPHKYKKRRPQDGKGKKDEGEESDTDEKCTICLSML--------ED 311

Query: 206 EKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 231
            ++V R+PC H++H+ C+ +WL +S  CP+CR ++E
Sbjct: 312 GEDVRRLPCMHLFHQLCVDQWLAMSKKCPICRVDIE 339

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BLAST of CSPI04G20690 vs. Swiss-Prot
Match: RN165_MOUSE (RING finger protein 165 OS=Mus musculus GN=Rnf165 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.4e-09
Identity = 33/96 (34.38%), Postives = 53/96 (55.21%), Query Frame = 1


Query: 146 VRKGASTSVIER-VMKEKY------DGNG----GEEKEGDDQCSVCYEQLYGKRENENEN 205
           V +GA  + IER     KY      D  G    GEE + D++C++C   L        E+
Sbjct: 253 VTRGAVQNTIERFTFPHKYKKRRPQDSKGKKDEGEESDTDEKCTICLSML--------ED 312

Query: 206 EKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 231
            ++V R+PC H++H+ C+ +WL +S  CP+CR ++E
Sbjct: 313 GEDVRRLPCMHLFHQLCVDQWLAMSKKCPICRVDIE 340

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BLAST of CSPI04G20690 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-08
Identity = 24/55 (43.64%), Postives = 33/55 (60.00%), Query Frame = 1


Query: 175 DQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRREL 230
           +QC+VC +          E   E  ++PC H+YHK C+L WL + NSCP+CR EL
Sbjct: 222 NQCAVCMDDF--------EEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

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BLAST of CSPI04G20690 vs. Swiss-Prot
Match: YGI4_SCHPO (Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPBC2A9.04c PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.9e-08
Identity = 28/77 (36.36%), Postives = 49/77 (63.64%), Query Frame = 1


Query: 155 IERVMKEKYDGNG--GEEKEGDDQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCI 214
           ++R +KE +D       ++  D  C +CY+ +     NEN +EK+ +++PCGH++ K+C+
Sbjct: 83  VKRAVKEAWDSFEPLSNDQLMDLTCPICYDDM-----NEN-DEKQATKMPCGHIFGKNCL 142

Query: 215 LKWLRISNSCPLCRREL 230
            KWL    +CPLCR+E+
Sbjct: 143 QKWLENHCTCPLCRKEV 153

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BLAST of CSPI04G20690 vs. Swiss-Prot
Match: RNG1L_ARATH (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 8.4e-08
Identity = 22/55 (40.00%), Postives = 36/55 (65.45%), Query Frame = 1


Query: 175 DQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRREL 230
           +QC+VC ++         E+  +V ++PC H++H+ C+L WL + NSCP+CR EL
Sbjct: 214 NQCAVCMDEF--------EDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

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BLAST of CSPI04G20690 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 1.9e-128
Identity = 229/231 (99.13%), Postives = 230/231 (99.57%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180
           FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELEE 232
           YEQLYGKRENENE EKEVSRIPCGHMYHKSCILKWL+ISNSCPLCRRELEE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of CSPI04G20690 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 5.9e-85
Identity = 169/230 (73.48%), Postives = 185/230 (80.43%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS P TFSF+CSIR N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180
           FHIIA +D VYTHWVDLDPAEDA AVR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180

Query: 181 YEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 231
           YE L+GK     E EKEVSRIPCGHM+HKSCILKWL+ISNSCPLCR ELE
Sbjct: 181 YEDLHGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of CSPI04G20690 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.5e-43
Identity = 116/232 (50.00%), Postives = 147/232 (63.36%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS     S  C +R     S+ T     Q V++RLR F +LV   S Q +QQT PT IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIPFSLWELE-DPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINV 120
            L  F L ELE + P + + FL +FLS  D +A     IF+NISSFA Q+   A  N+N+
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFLSPADSAA-----IFHNISSFALQL---AAGNLNL 120

Query: 121 NFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYD-GNGGEEKEGDDQ 180
           NFH+IA VD++YT  ++L+  P + +P VR G   SV+ER+M EKYD GNGGE   G  Q
Sbjct: 121 NFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGG--Q 180

Query: 181 CSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRE 229
           C VCYE L  +RE E E   E +RIPCGH+YHKSCILKWL ++NSCPLCR E
Sbjct: 181 CCVCYEDLNCEREEEKE---EATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of CSPI04G20690 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-25
Identity = 87/193 (45.08%), Postives = 111/193 (57.51%), Query Frame = 1


Query: 49  ILQQTSPTLISDTLIPFSLWELEDPPFISY--FFLFRFLSSYDISAAVCDAIFYNISSFA 108
           IL+QT  TLI + L  FSL  L+ P       + +  FL S+++ ++VC  + +NI SFA
Sbjct: 47  ILEQTPFTLIGEAL--FSLSNLQYPLQQQELEYMVNEFLRSFNLRSSVCRIVSHNILSFA 106

Query: 109 GQILLAADSNINVNFHIIADVDIVYTHWVDLDPA---------EDAPAVRKGASTSVIER 168
            Q     D N N  F I  +VD +  +W +  P          E  P   +GAS S IER
Sbjct: 107 QQF----DIN-NECFKIETNVDYIKEYWTEAAPELYPISTVGEEGLPP--RGASESAIER 166

Query: 169 VMKEKYDGNGGEEKEGDDQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLR 228
           V K+K+DG  GEE+E    CSVC E++  KR+       EV RIPCGH+YHKSCILKWL 
Sbjct: 167 VKKQKFDGFRGEEEEEIGDCSVCCEEI--KRKG-----GEVRRIPCGHVYHKSCILKWLE 223

Query: 229 ISNSCPLCRRELE 231
           ISNSCPLCR  LE
Sbjct: 227 ISNSCPLCRAALE 223

[Close]

BLAST of CSPI04G20690 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.9e-17
Identity = 69/196 (35.20%), Postives = 97/196 (49.49%), Query Frame = 1


Query: 48  QILQQTSPTLISDTLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAG 107
           ++L QT      + L   SL +L+ P     F++ + LSS +I  A    +   I+S   
Sbjct: 46  EVLHQTPRIPYHNALFYLSLRQLQSP----LFYITQILSSLNIRPAASQNMARRIAS--- 105

Query: 108 QILLAADSNINVNFHIIADVDIVYTHW--------------VDLDPAEDAPAVRKGASTS 167
            IL   D N   NFHI+A+VD +   W              V LD A   PAV++G   +
Sbjct: 106 HILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEAP--PAVKRGVGVA 165

Query: 168 VIERVMKEKYDGNGGEEKEGDDQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCIL 227
             ER+  E       E+ E    CS+C ++L       +  ++EV RIPCGH+YH+SCI 
Sbjct: 166 RGERLRSE-------EKMEELGDCSICLDEL-------SCEKREVMRIPCGHVYHESCIF 218

Query: 228 KWLRISNSCPLCRREL 230
           KWL   NSCPLCR+ L
Sbjct: 226 KWLENHNSCPLCRKPL 218

[Close]

BLAST of CSPI04G20690 vs. TAIR10
Match: AT5G37200.1 (AT5G37200.1 RING/U-box superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 8.6e-11
Identity = 37/125 (29.60%), Postives = 66/125 (52.80%), Query Frame = 1


Query: 104 SFAGQILLAADSNINVNFHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKY 163
           SFA    L     + V    I +  +++ H   L        + +GAS  V  R+++E+ 
Sbjct: 95  SFANDNSLRQPVTVTVYVTYIKERRVIFPHGPSL--------LSRGASGEVFHRLVEEQR 154

Query: 164 DGNGGEEKEGDDQCSVCYEQLYGKRENENENEKEVSRIP-CGHMYHKSCILKWLRISNSC 223
             +   E+E D+ CS+C E+        +E+ +++ R+P C H++H+ C+ +WL + NSC
Sbjct: 155 VESADLEEE-DETCSICIEKF-------SESHEDIIRVPDCLHLFHQGCLFEWLGLQNSC 203

Query: 224 PLCRR 228
           PLCR+
Sbjct: 215 PLCRK 203

[Close]

BLAST of CSPI04G20690 vs. TAIR10
Match: AT5G67120.1 (AT5G67120.1 RING/U-box superfamily protein)

HSP 1 Score: 63.5 bits (153), Expect = 1.9e-10
Identity = 34/101 (33.66%), Postives = 57/101 (56.44%), Query Frame = 1


Query: 130 VYTHWVDLDPAEDAPAVRKGASTSVI-ERVMKEKYDGNGGEEKEGDDQCSVCYEQLYGKR 189
           ++T+   L   ++    R G S  VI E +++ KY+    +E +   +C +C ++L   +
Sbjct: 182 IFTYEELLSITDETGDERTGLSEEVIDENLIRRKYEKRSDDETK---RCVICQQKL---K 241

Query: 190 ENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRREL 230
           +NE     EVS++ CGH +H  CI  WL ++N CPLC RE+
Sbjct: 242 DNE-----EVSKLGCGHDFHFGCIKNWLMVTNKCPLCNREV 271

[Close]

BLAST of CSPI04G20690 vs. TAIR10
Match: AT1G60360.1 (AT1G60360.1 RING/U-box superfamily protein)

HSP 1 Score: 62.0 bits (149), Expect = 5.6e-10
Identity = 24/54 (44.44%), Postives = 35/54 (64.81%), Query Frame = 1


Query: 176 QCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRREL 230
           QC+VC E+     +         + +PC H+YHK CI+ WLR++NSCP+CRR+L
Sbjct: 223 QCTVCMEEFIVGGD--------ATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

[Close]

BLAST of CSPI04G20690 vs. TAIR10
Match: AT5G10650.1 (AT5G10650.1 RING/U-box superfamily protein)

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-09
Identity = 26/61 (42.62%), Postives = 36/61 (59.02%), Query Frame = 1


Query: 171 KEGDDQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 230
           K+ D +CS+C E        E  +  E+  IPC HMYH SC+ +WLR+ N CP+C+   E
Sbjct: 469 KDDDIKCSICQE--------EYVDGDELGTIPCQHMYHVSCVQQWLRMKNWCPICKTSAE 521

Query: 231 E 232
           E
Sbjct: 529 E 521

[Close]

BLAST of CSPI04G20690 vs. TAIR10
Match: AT3G02340.1 (AT3G02340.1 RING/U-box superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 3.6e-09
Identity = 25/61 (40.98%), Postives = 38/61 (62.30%), Query Frame = 1


Query: 169 EEKEGDDQCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRE 228
           E  +G++ C+VC +++          E++V R+PC H YH  CI+ WL I N+CP+CR E
Sbjct: 327 ELDKGNNVCAVCKDEMLV--------EEKVRRLPCSHFYHGECIIPWLGIRNTCPVCRYE 379

Query: 229 L 230
           L
Sbjct: 387 L 379

[Close]

BLAST of CSPI04G20690 vs. NCBI nr
Match: gi|700199693|gb|KGN54851.1| (hypothetical protein Csa_4G551140 [Cucumis sativus])

HSP 1 Score: 466.5 bits (1199), Expect = 2.8e-128
Identity = 229/231 (99.13%), Postives = 230/231 (99.57%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180
           FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 YEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELEE 232
           YEQLYGKRENENE EKEVSRIPCGHMYHKSCILKWL+ISNSCPLCRRELEE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of CSPI04G20690 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 322.0 bits (824), Expect = 8.5e-85
Identity = 169/230 (73.48%), Postives = 185/230 (80.43%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS P TFSF+CSIR N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180
           FHIIA +D VYTHWVDLDPAEDA AVR+GA  S IER+MKEKYDGNGGEE E  D+CSVC
Sbjct: 121 FHIIAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEME--DECSVC 180

Query: 181 YEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 231
           YE L+GK     E EKEVSRIPCGHM+HKSCILKWL+ISNSCPLCR ELE
Sbjct: 181 YEDLHGK----TEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of CSPI04G20690 vs. NCBI nr
Match: gi|659132530|ref|XP_008466247.1| (PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo])

HSP 1 Score: 207.6 bits (527), Expect = 2.3e-50
Identity = 126/232 (54.31%), Postives = 152/232 (65.52%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS   T S +C I     SS+AT     Q V++RLR + +LV   S QI+QQT PT ISD
Sbjct: 1   MSSTTTLSLECCIH----SSVATTADE-QLVEIRLRRYSKLVSPSSNQIIQQTFPTPISD 60

Query: 61  TLIPFSL--WELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNIN 120
            L  F L   ELE+PPF  +  L +FLS  D +A     IF+NISSFA Q+   A  N+N
Sbjct: 61  ALFHFPLPELELENPPFFFHDLLLQFLSPADSTA-----IFHNISSFALQL---AAGNLN 120

Query: 121 VNFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQ 180
           +NFH+IA VD++YT  VDLD  P + +P VR GA  SVIER+M EKYD   G E+ GD Q
Sbjct: 121 LNFHLIATVDLLYTISVDLDLDPPQYSPLVRNGAPASVIERLMDEKYDDGNGREEMGD-Q 180

Query: 181 CSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRE 229
           C VCYE L    E E E E+EV+RIPCGH+YHK CILKWL I+NSCPLCRRE
Sbjct: 181 CCVCYEDL--NCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNSCPLCRRE 216

[Close]

BLAST of CSPI04G20690 vs. NCBI nr
Match: gi|778689036|ref|XP_011652885.1| (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus])

HSP 1 Score: 184.5 bits (467), Expect = 2.1e-43
Identity = 116/232 (50.00%), Postives = 147/232 (63.36%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS     S  C +R     S+ T     Q V++RLR F +LV   S Q +QQT PT IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIPFSLWELE-DPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINV 120
            L  F L ELE + P + + FL +FLS  D +A     IF+NISSFA Q+   A  N+N+
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFLSPADSAA-----IFHNISSFALQL---AAGNLNL 120

Query: 121 NFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYD-GNGGEEKEGDDQ 180
           NFH+IA VD++YT  ++L+  P + +P VR G   SV+ER+M EKYD GNGGE   G  Q
Sbjct: 121 NFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGG--Q 180

Query: 181 CSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRE 229
           C VCYE L  +RE E E   E +RIPCGH+YHKSCILKWL ++NSCPLCR E
Sbjct: 181 CCVCYEDLNCEREEEKE---EATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of CSPI04G20690 vs. NCBI nr
Match: gi|659121671|ref|XP_008460768.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo])

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-26
Identity = 96/235 (40.85%), Postives = 128/235 (54.47%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MSL  + SFQCS      +          F+  R  +  +L+     QILQQT   L+ +
Sbjct: 1   MSLSTSSSFQCSFSATPNTD--------HFLICRFGYSSRLIIV--DQILQQTPSILMGE 60

Query: 61  TLIPFS-LWELEDPPFISYFFLFRFLSSYDIS--AAVCDAIFYNISSFAGQILLAADSNI 120
           TLIPFS LW  E    I  F +  FL S+++   A+    I   I SFA Q       N+
Sbjct: 61  TLIPFSNLWHPEQQQSIE-FLVNEFLGSFNLHSFASARRTICRKILSFALQF-----HNV 120

Query: 121 NVNFHIIADVDIVYTHWVDLDPAEDAPAVRK--GASTSVIERVMKEKYDGNGGEEKEGDD 180
           + +F I A+V+ V    +     ++ P+  +  GA  S IER+ KEK+D    EE+ GD 
Sbjct: 121 HGSFKIEANVEHVEDLIIGEQGFQETPSPPRTTGAPESAIERLRKEKFDVLNKEEEIGD- 180

Query: 181 QCSVCYEQLYGKRENENENEKEVSRIPCGHMYHKSCILKWLRISNSCPLCRRELE 231
            CSVCYE+L G+        KEVSRIPCGH+YHKSCIL WL+I+NSCPLCR  L+
Sbjct: 181 -CSVCYEELKGQG-------KEVSRIPCGHVYHKSCILTWLQINNSCPLCRAPLQ 210

The following BLAST results are available for this feature:

BLAST of CSPI04G20690 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RN165_HUMAN	6.9e-10	35.42	RING finger protein 165 OS=Homo sapiens GN=RNF165 PE=1 SV=1	[more]
RN165_MOUSE	3.4e-09	34.38	RING finger protein 165 OS=Mus musculus GN=Rnf165 PE=2 SV=1	[more]
RING1_GOSHI	1.3e-08	43.64	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
YGI4_SCHPO	2.9e-08	36.36	Uncharacterized RING finger protein C2A9.04c OS=Schizosaccharomyces pombe (strai...	[more]
RNG1L_ARATH	8.4e-08	40.00	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana GN=At3g19950 PE=1...	[more]

BLAST of CSPI04G20690 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3Z2_CUCSA	1.9e-128	99.13	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1	[more]
A0A0A0LRB0_CUCSA	5.9e-85	73.48	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1	[more]
A0A0A0LHI3_CUCSA	1.5e-43	50.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1	[more]
A0A0A0LHS2_CUCSA	1.4e-25	45.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1	[more]
A0A0A0K2W2_CUCSA	6.9e-17	35.20	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1	[more]

BLAST of CSPI04G20690 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G37200.1	8.6e-11	29.60	RING/U-box superfamily protein	[more]
AT5G67120.1	1.9e-10	33.66	RING/U-box superfamily protein	[more]
AT1G60360.1	5.6e-10	44.44	RING/U-box superfamily protein	[more]
AT5G10650.1	1.6e-09	42.62	RING/U-box superfamily protein	[more]
AT3G02340.1	3.6e-09	40.98	RING/U-box superfamily protein	[more]

BLAST of CSPI04G20690 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700199693\|gb\|KGN54851.1\|	2.8e-128	99.13	hypothetical protein Csa_4G551140 [Cucumis sativus]	[more]
gi\|778674460\|ref\|XP_011650219.1\|	8.5e-85	73.48	PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus]	[more]
gi\|659132530\|ref\|XP_008466247.1\|	2.3e-50	54.31	PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo]	[more]
gi\|778689036\|ref\|XP_011652885.1\|	2.1e-43	50.00	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus]	[more]
gi\|659121671\|ref\|XP_008460768.1\|	1.4e-26	40.85	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20690.1	CSPI04G20690.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 175..226 score: 1.6
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..225 score: 1.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 177..226 score: 1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 168..230 score: 1.6
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 167..230 score: 5.7
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 171..229 score: 1.52

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G20690	Csa4G551140	Cucumber (Chinese Long) v2	cpicuB181
CSPI04G20690	Lsi01G019550	Bottle gourd (USVL1VR-Ls)	cpilsiB258
CSPI04G20690	CsaV3_4G031160	Cucumber (Chinese Long) v3	cpicucB212
CSPI04G20690	Cla97C04G069460	Watermelon (97103) v2	cpiwmbB306
CSPI04G20690	Bhi03G002247	Wax gourd	cpiwgoB400
CSPI04G20690	Cucsa.357530	Cucumber (Gy14) v1	cgycpiB529
CSPI04G20690	CmoCh03G001250	Cucurbita moschata (Rifu)	cmocpiB670
CSPI04G20690	CsGy4G019420	Cucumber (Gy14) v2	cgybcpiB169

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G20690	Watermelon (97103) v2	cpiwmbB308
CSPI04G20690	Watermelon (97103) v2	cpiwmbB314
CSPI04G20690	Cucurbita maxima (Rimu)	cmacpiB679
CSPI04G20690	Cucurbita maxima (Rimu)	cmacpiB878
CSPI04G20690	Cucurbita moschata (Rifu)	cmocpiB858
CSPI04G20690	Melon (DHL92) v3.5.1	cpimeB333
CSPI04G20690	Watermelon (Charleston Gray)	cpiwcgB319
CSPI04G20690	Watermelon (97103) v1	cpiwmB338
CSPI04G20690	Watermelon (97103) v1	cpiwmB349
CSPI04G20690	Cucurbita pepo (Zucchini)	cpecpiB066
CSPI04G20690	Cucurbita pepo (Zucchini)	cpecpiB500
CSPI04G20690	Melon (DHL92) v3.6.1	cpimedB324
CSPI04G20690	Silver-seed gourd	carcpiB0315
CSPI04G20690	Silver-seed gourd	carcpiB0894