Cucsa.357530 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.357530
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	RING/U-box superfamily protein
Location	scaffold03578 : 648412 .. 649107 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTtCTCTCTTCCTACGATATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGGTGATGATCAATGCAGTGTTTGTTATGAACAACTGTATGGAAAAAGAGAGAATGAAAACGAAAaGGAAAaGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

mRNA sequence

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTTCTCTCTTCCTACGATATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGAGAATGAAAACGAAAAGGAAAAGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Coding sequence (CDS)

ATGTCTCTCCCAATCACTTTCTCCTTCCAATGCTCTATTCGCCGCAACGGCAAGAGCAGTCTTGCCACCGACGGTCAACCGTTGCAATTCGTCCAACTCCGTTTGAGGCACTTCGGCCAATTGGTTCATACACCTTCCCAGCAAATCCTTCAACAAACTTCCCCCACTTTAATTTCCGATACTCTGATCCCTTTCTCTCTGTGGGAGCTTGAAGATCCACCTTTCATCTCCTACTTCTTCCTCTTTCGTTTtCTCTCTTCCTACGATATCTCCGCCGCCGTCTGTGACGCCATCTTCTACAATATTTCCTCCTTTGCTGGTCAAATATTATTGGCCGCCGATAGCAACATAAATGTCAACTTCCACATCATAGCCGATGTGGATATCGTGTACACGCACTGGGTGGACCTGGACCCAGCAGAAGATGCCCCGGCGGTAAGGAAAGGGGCGTCAACATCGGTGATTGAGAGGGTGATGAAGGAGAAATATGATGGGAATGGAGGGGAAGAGAAGGAAGAGAATGAAAACGAAAaGGAAAaGGAAGTGAGCAGAATCCCATGCGGGCACATGTATCATAAGTCATGCATTCTCAAATGGCTTAAAATCAGCAATTCATGTCCTTTGTGCAGAAGGGAATTAGAAGAATAA

Protein sequence

MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISDTLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVNFHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE*

Homology

[Close]

BLAST of Cucsa.357530 vs. Swiss-Prot
Match: RING1_GOSHI (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.9e-07
Identity = 20/37 (54.05%), Postives = 28/37 (75.68%), Query Frame = 1


Query: 177 EKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           E+  E  ++PC H+YHK C+L WL++ NSCP+CR EL
Sbjct: 232 EEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHEL 268

[Close]

BLAST of Cucsa.357530 vs. Swiss-Prot
Match: RN181_RAT (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-07
Identity = 22/39 (56.41%), Postives = 29/39 (74.36%), Query Frame = 1


Query: 175 ENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           E E+E+ V  +PC H++H +CIL WL  +NSCPLCR EL
Sbjct: 94  EFEEEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHEL 132

[Close]

BLAST of Cucsa.357530 vs. Swiss-Prot
Match: RN181_XENTR (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.1e-07
Identity = 31/88 (35.23%), Postives = 45/88 (51.14%), Query Frame = 1


Query: 126 DVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEENENEKEKEVSRI 185
           D+D+    + + D     PA +K   +     V  E+ D          E E+ + V ++
Sbjct: 36  DIDLGALDFTEWDQRLPPPAAKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQL 95

Query: 186 PCGHMYHKSCILKWLKISNSCPLCRREL 214
           PC H++H SCIL WL  +NSCPLCR EL
Sbjct: 96  PCEHLFHSSCILPWLGKTNSCPLCRHEL 123

[Close]

BLAST of Cucsa.357530 vs. Swiss-Prot
Match: RN181_HUMAN (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens GN=RNF181 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.6e-07
Identity = 28/67 (41.79%), Postives = 40/67 (59.70%), Query Frame = 1


Query: 150 ASTSVIERVMKEKYDGNGGEEKEEN---ENEKEKEVSRIPCGHMYHKSCILKWLKISNSC 209
           A+ +V+E + +    G+  E K      E E+E+    +PC H++H SCIL WL  +NSC
Sbjct: 54  AAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSC 113

Query: 210 PLCRREL 214
           PLCR EL
Sbjct: 114 PLCRYEL 120

[Close]

BLAST of Cucsa.357530 vs. Swiss-Prot
Match: RN181_MOUSE (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.6e-07
Identity = 22/39 (56.41%), Postives = 28/39 (71.79%), Query Frame = 1


Query: 175 ENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           E E E+ V  +PC H++H +CIL WL  +NSCPLCR EL
Sbjct: 94  EFEAEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHEL 132

[Close]

BLAST of Cucsa.357530 vs. TrEMBL
Match: A0A0A0L3Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 3.2e-117
Identity = 215/231 (93.07%), Postives = 215/231 (93.07%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKE-------- 180
           FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKE        
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 --------ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 216
                   ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of Cucsa.357530 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 3.7e-81
Identity = 161/224 (71.88%), Postives = 176/224 (78.57%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS P TFSF+CSIR N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEE------- 180
           FHIIA +D VYTHWVDLDPAEDA AVR+GA  S IER+MKEKYDGNGGEE E+       
Sbjct: 121 FHIIAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYE 180

Query: 181 ---NENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 215
               + EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 DLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of Cucsa.357530 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 7.0e-40
Identity = 109/227 (48.02%), Postives = 140/227 (61.67%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS     S  C +R     S+ T     Q V++RLR F +LV   S Q +QQT PT IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIPFSLWELE-DPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINV 120
            L  F L ELE + P + + FL +FLS  D +A     IF+NISSFA Q+   A  N+N+
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFLSPADSAA-----IFHNISSFALQL---AAGNLNL 120

Query: 121 NFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYD-GNGGE------- 180
           NFH+IA VD++YT  ++L+  P + +P VR G   SV+ER+M EKYD GNGGE       
Sbjct: 121 NFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCC 180

Query: 181 ---EKEENENEKEKE-VSRIPCGHMYHKSCILKWLKISNSCPLCRRE 213
              E    E E+EKE  +RIPCGH+YHKSCILKWL ++NSCPLCR E
Sbjct: 181 VCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of Cucsa.357530 vs. TrEMBL
Match: A0A0A0LHS2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-24
Identity = 84/186 (45.16%), Postives = 106/186 (56.99%), Query Frame = 1


Query: 49  ILQQTSPTLISDTLIPFSLWELEDPPFISY--FFLFRFLSSYDISAAVCDAIFYNISSFA 108
           IL+QT  TLI + L  FSL  L+ P       + +  FL S+++ ++VC  + +NI SFA
Sbjct: 47  ILEQTPFTLIGEAL--FSLSNLQYPLQQQELEYMVNEFLRSFNLRSSVCRIVSHNILSFA 106

Query: 109 GQILLAADSNINVNFHIIADVDIVYTHWVDLDPA---------EDAPAVRKGASTSVIER 168
            Q     D N N  F I  +VD +  +W +  P          E  P   +GAS S IER
Sbjct: 107 QQF----DIN-NECFKIETNVDYIKEYWTEAAPELYPISTVGEEGLPP--RGASESAIER 166

Query: 169 VMKEKYDGNGGEEKEEN-------ENEKEK--EVSRIPCGHMYHKSCILKWLKISNSCPL 215
           V K+K+DG  GEE+EE        E  K K  EV RIPCGH+YHKSCILKWL+ISNSCPL
Sbjct: 167 VKKQKFDGFRGEEEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPL 223

[Close]

BLAST of Cucsa.357530 vs. TrEMBL
Match: A0A0A0K696_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.2e-16
Identity = 64/182 (35.16%), Postives = 93/182 (51.10%), Query Frame = 1


Query: 48  QILQQTSPTLISDTLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAG 107
           ++L QT      + L   SL +L+ P     F++ + LSS +I  A    +   I+S   
Sbjct: 46  EVLHQTPRIPYHNALFYLSLRQLQSP----LFYITQILSSLNIRPAASQNMARRIAS--- 105

Query: 108 QILLAADSNINVNFHIIADVDIVYTHW--------------VDLDPAEDAPAVRKGASTS 167
            IL   D N   NFHI+A+VD +   W              V LD A   PAV++G   +
Sbjct: 106 HILQMPDRNSETNFHILAEVDFIRLIWLPSMGGGGAGAEETVVLDEAP--PAVKRGVGVA 165

Query: 168 VIERVMKEKYDGNGGEEKE--ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRR 214
             ER+  E+     G+     +  + +++EV RIPCGH+YH+SCI KWL+  NSCPLCR+
Sbjct: 166 RGERLRSEEKMEELGDCSICLDELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK 218

[Close]

BLAST of Cucsa.357530 vs. TAIR10
Match: AT5G67120.1 (AT5G67120.1 RING/U-box superfamily protein)

HSP 1 Score: 58.9 bits (141), Expect = 4.4e-09
Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1


Query: 130 VYTHWVDLDPAEDAPAVRKGASTSVI-ERVMKEKYDGNGGEEKE-----ENENEKEKEVS 189
           ++T+   L   ++    R G S  VI E +++ KY+    +E +     + + +  +EVS
Sbjct: 182 IFTYEELLSITDETGDERTGLSEEVIDENLIRRKYEKRSDDETKRCVICQQKLKDNEEVS 241

Query: 190 RIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           ++ CGH +H  CI  WL ++N CPLC RE+
Sbjct: 242 KLGCGHDFHFGCIKNWLMVTNKCPLCNREV 271

[Close]

BLAST of Cucsa.357530 vs. TAIR10
Match: AT3G02340.1 (AT3G02340.1 RING/U-box superfamily protein)

HSP 1 Score: 57.8 bits (138), Expect = 9.8e-09
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 1


Query: 154 VIERVMKEKYDGNGGEEKEENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 213
           V+E  ++E   GN      ++E   E++V R+PC H YH  CI+ WL I N+CP+CR EL
Sbjct: 320 VVELAVEELDKGNNVCAVCKDEMLVEEKVRRLPCSHFYHGECIIPWLGIRNTCPVCRYEL 379

[Close]

BLAST of Cucsa.357530 vs. TAIR10
Match: AT5G37200.1 (AT5G37200.1 RING/U-box superfamily protein)

HSP 1 Score: 56.6 bits (135), Expect = 2.2e-08
Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 1


Query: 104 SFAGQILLAADSNINVNFHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKY 163
           SFA    L     + V    I +  +++ H   L        + +GAS  V  R+++E+ 
Sbjct: 95  SFANDNSLRQPVTVTVYVTYIKERRVIFPHGPSL--------LSRGASGEVFHRLVEEQR 154

Query: 164 DGNGGEEKE--------ENENEKEKEVSRIP-CGHMYHKSCILKWLKISNSCPLCRR 212
             +   E+E        E  +E  +++ R+P C H++H+ C+ +WL + NSCPLCR+
Sbjct: 155 VESADLEEEDETCSICIEKFSESHEDIIRVPDCLHLFHQGCLFEWLGLQNSCPLCRK 203

[Close]

BLAST of Cucsa.357530 vs. TAIR10
Match: AT1G60360.1 (AT1G60360.1 RING/U-box superfamily protein)

HSP 1 Score: 56.2 bits (134), Expect = 2.8e-08
Identity = 18/33 (54.55%), Postives = 28/33 (84.85%), Query Frame = 1


Query: 181 EVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           + + +PC H+YHK CI+ WL+++NSCP+CRR+L
Sbjct: 236 DATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

[Close]

BLAST of Cucsa.357530 vs. TAIR10
Match: AT1G68180.1 (AT1G68180.1 RING/U-box superfamily protein)

HSP 1 Score: 55.8 bits (133), Expect = 3.7e-08
Identity = 39/99 (39.39%), Postives = 51/99 (51.52%), Query Frame = 1


Query: 128 DIVYTHWVDLDPAEDAPAVRKG---ASTSVIERV----------MKEKYDGNGGEEKEEN 187
           D+VY    D + A+  P+V+ G   AS S IE V          +KEK      EE E  
Sbjct: 91  DLVYN--TDEEFADVMPSVQIGPPPASQSAIEAVRTVIITDEDLVKEKVCAICKEEFEVG 150

Query: 188 ENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRREL 214
           E  KE     + C H+YH SCI+ WL I N+CP+CR E+
Sbjct: 151 EEGKE-----LKCLHLYHSSCIVSWLNIHNTCPICRFEV 182

[Close]

BLAST of Cucsa.357530 vs. NCBI nr
Match: gi|700199693|gb|KGN54851.1| (hypothetical protein Csa_4G551140 [Cucumis sativus])

HSP 1 Score: 429.1 bits (1102), Expect = 4.6e-117
Identity = 215/231 (93.07%), Postives = 215/231 (93.07%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD
Sbjct: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN
Sbjct: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKE-------- 180
           FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKE        
Sbjct: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEGDDQCSVC 180

Query: 181 --------ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 216
                   ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE
Sbjct: 181 YEQLYGKRENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELEE 231

[Close]

BLAST of Cucsa.357530 vs. NCBI nr
Match: gi|778674460|ref|XP_011650219.1| (PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 5.3e-81
Identity = 161/224 (71.88%), Postives = 176/224 (78.57%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS P TFSF+CSIR N   S AT+GQPLQFV+LRL HF QLV +PS QILQQTSP LISD
Sbjct: 1   MSSPTTFSFRCSIRCNANGSAATNGQPLQFVELRLGHFSQLVLSPSHQILQQTSPILISD 60

Query: 61  TLIPFSLWELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINVN 120
           TLI F L ELEDPPFIS+  L RFLSSY+IS AVCDAIF  ISSFA Q++     N+N N
Sbjct: 61  TLIRFPLRELEDPPFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVA---GNLNSN 120

Query: 121 FHIIADVDIVYTHWVDLDPAEDAPAVRKGASTSVIERVMKEKYDGNGGEEKEE------- 180
           FHIIA +D VYTHWVDLDPAEDA AVR+GA  S IER+MKEKYDGNGGEE E+       
Sbjct: 121 FHIIAALDFVYTHWVDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYE 180

Query: 181 ---NENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRELE 215
               + EKEKEVSRIPCGHM+HKSCILKWLKISNSCPLCR ELE
Sbjct: 181 DLHGKTEKEKEVSRIPCGHMFHKSCILKWLKISNSCPLCRGELE 221

[Close]

BLAST of Cucsa.357530 vs. NCBI nr
Match: gi|659132530|ref|XP_008466247.1| (PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo])

HSP 1 Score: 197.2 bits (500), Expect = 2.9e-47
Identity = 121/229 (52.84%), Postives = 146/229 (63.76%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS   T S +C I     SS+AT     Q V++RLR + +LV   S QI+QQT PT ISD
Sbjct: 1   MSSTTTLSLECCIH----SSVATTADE-QLVEIRLRRYSKLVSPSSNQIIQQTFPTPISD 60

Query: 61  TLIPFSL--WELEDPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNIN 120
            L  F L   ELE+PPF  +  L +FLS  D +A     IF+NISSFA Q+   A  N+N
Sbjct: 61  ALFHFPLPELELENPPFFFHDLLLQFLSPADSTA-----IFHNISSFALQL---AAGNLN 120

Query: 121 VNFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYD-GNGGEEKE--- 180
           +NFH+IA VD++YT  VDLD  P + +P VR GA  SVIER+M EKYD GNG EE     
Sbjct: 121 LNFHLIATVDLLYTISVDLDLDPPQYSPLVRNGAPASVIERLMDEKYDDGNGREEMGDQC 180

Query: 181 ---------ENENEKEKEVSRIPCGHMYHKSCILKWLKISNSCPLCRRE 213
                    E E EKE+EV+RIPCGH+YHK CILKWL I+NSCPLCRRE
Sbjct: 181 CVCYEDLNCEREEEKEEEVTRIPCGHVYHKYCILKWLNINNSCPLCRRE 216

[Close]

BLAST of Cucsa.357530 vs. NCBI nr
Match: gi|778689036|ref|XP_011652885.1| (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus])

HSP 1 Score: 172.2 bits (435), Expect = 1.0e-39
Identity = 109/227 (48.02%), Postives = 140/227 (61.67%), Query Frame = 1


Query: 1   MSLPITFSFQCSIRRNGKSSLATDGQPLQFVQLRLRHFGQLVHTPSQQILQQTSPTLISD 60
           MS     S  C +R     S+ T     Q V++RLR F +LV   S Q +QQT PT IS+
Sbjct: 1   MSSTTNLSLDCCVR-----SIPTTADK-QLVEIRLRRFSKLVSPFSNQNIQQTFPTSISN 60

Query: 61  TLIPFSLWELE-DPPFISYFFLFRFLSSYDISAAVCDAIFYNISSFAGQILLAADSNINV 120
            L  F L ELE + P + + FL +FLS  D +A     IF+NISSFA Q+   A  N+N+
Sbjct: 61  ALFHFPLPELELENPSLFHDFLLQFLSPADSAA-----IFHNISSFALQL---AAGNLNL 120

Query: 121 NFHIIADVDIVYTHWVDLD--PAEDAPAVRKGASTSVIERVMKEKYD-GNGGE------- 180
           NFH+IA VD++YT  ++L+  P + +P VR G   SV+ER+M EKYD GNGGE       
Sbjct: 121 NFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEKYDDGNGGEGMGGQCC 180

Query: 181 ---EKEENENEKEKE-VSRIPCGHMYHKSCILKWLKISNSCPLCRRE 213
              E    E E+EKE  +RIPCGH+YHKSCILKWL ++NSCPLCR E
Sbjct: 181 VCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCRSE 213

[Close]

BLAST of Cucsa.357530 vs. NCBI nr
Match: gi|449442467|ref|XP_004139003.1| (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 2.0e-24
Identity = 84/186 (45.16%), Postives = 106/186 (56.99%), Query Frame = 1


Query: 49  ILQQTSPTLISDTLIPFSLWELEDPPFISY--FFLFRFLSSYDISAAVCDAIFYNISSFA 108
           IL+QT  TLI + L  FSL  L+ P       + +  FL S+++ ++VC  + +NI SFA
Sbjct: 47  ILEQTPFTLIGEAL--FSLSNLQYPLQQQELEYMVNEFLRSFNLRSSVCRIVSHNILSFA 106

Query: 109 GQILLAADSNINVNFHIIADVDIVYTHWVDLDPA---------EDAPAVRKGASTSVIER 168
            Q     D N N  F I  +VD +  +W +  P          E  P   +GAS S IER
Sbjct: 107 QQF----DIN-NECFKIETNVDYIKEYWTEAAPELYPISTVGEEGLPP--RGASESAIER 166

Query: 169 VMKEKYDGNGGEEKEEN-------ENEKEK--EVSRIPCGHMYHKSCILKWLKISNSCPL 215
           V K+K+DG  GEE+EE        E  K K  EV RIPCGH+YHKSCILKWL+ISNSCPL
Sbjct: 167 VKKQKFDGFRGEEEEEIGDCSVCCEEIKRKGGEVRRIPCGHVYHKSCILKWLEISNSCPL 223

The following BLAST results are available for this feature:

BLAST of Cucsa.357530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RING1_GOSHI	3.9e-07	54.05	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum GN=RING1 PE=1 SV=1	[more]
RN181_RAT	5.1e-07	56.41	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1	[more]
RN181_XENTR	5.1e-07	35.23	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1	[more]
RN181_HUMAN	6.6e-07	41.79	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens GN=RNF181 PE=1 SV=1	[more]
RN181_MOUSE	8.6e-07	56.41	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1	[more]

BLAST of Cucsa.357530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3Z2_CUCSA	3.2e-117	93.07	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G551140 PE=4 SV=1	[more]
A0A0A0LRB0_CUCSA	3.7e-81	71.88	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1	[more]
A0A0A0LHI3_CUCSA	7.0e-40	48.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1	[more]
A0A0A0LHS2_CUCSA	1.4e-24	45.16	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G130680 PE=4 SV=1	[more]
A0A0A0K696_CUCSA	4.2e-16	35.16	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1	[more]

BLAST of Cucsa.357530 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G67120.1	4.4e-09	32.22	RING/U-box superfamily protein	[more]
AT3G02340.1	9.8e-09	43.33	RING/U-box superfamily protein	[more]
AT5G37200.1	2.2e-08	28.21	RING/U-box superfamily protein	[more]
AT1G60360.1	2.8e-08	54.55	RING/U-box superfamily protein	[more]
AT1G68180.1	3.7e-08	39.39	RING/U-box superfamily protein	[more]

BLAST of Cucsa.357530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700199693\|gb\|KGN54851.1\|	4.6e-117	93.07	hypothetical protein Csa_4G551140 [Cucumis sativus]	[more]
gi\|778674460\|ref\|XP_011650219.1\|	5.3e-81	71.88	PREDICTED: RING-H2 finger protein ATL16-like [Cucumis sativus]	[more]
gi\|659132530\|ref\|XP_008466247.1\|	2.9e-47	52.84	PREDICTED: uncharacterized protein LOC103503717 [Cucumis melo]	[more]
gi\|778689036\|ref\|XP_011652885.1\|	1.0e-39	48.02	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus]	[more]
gi\|449442467\|ref\|XP_004139003.1\|	2.0e-24	45.16	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.357530.1	Cucsa.357530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 177..210 score: 6.3
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 186..210 score: 9
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 169..214 score: 2.1
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 177..213 score: 1.4E-12coord: 10..18 score: 1.4
None	No IPR available	PANTHER	PTHR22763:SF38	SUBFAMILY NOT NAMED	coord: 10..18 score: 1.4E-12coord: 177..213 score: 1.4
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 177..213 score: 5.96

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.357530	Csa4G551140	Cucumber (Chinese Long) v2	cgycuB501
Cucsa.357530	MELO3C009075	Melon (DHL92) v3.5.1	cgymeB577
Cucsa.357530	CSPI04G20690	Wild cucumber (PI 183967)	cgycpiB529
Cucsa.357530	CmoCh03G001250	Cucurbita moschata (Rifu)	cgycmoB0974
Cucsa.357530	Lsi01G019550	Bottle gourd (USVL1VR-Ls)	cgylsiB575
Cucsa.357530	MELO3C009075.2	Melon (DHL92) v3.6.1	cgymedB578
Cucsa.357530	CsaV3_4G031160	Cucumber (Chinese Long) v3	cgycucB543
Cucsa.357530	Bhi03G002247	Wax gourd	cgywgoB713

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.357530	Cucurbita pepo (Zucchini)	cgycpeB0931
Cucsa.357530	Cucurbita pepo (Zucchini)	cgycpeB0932
Cucsa.357530	Melon (DHL92) v3.6.1	cgymedB577
Cucsa.357530	Silver-seed gourd	carcgyB0336
Cucsa.357530	Silver-seed gourd	carcgyB0979
Cucsa.357530	Watermelon (97103) v2	cgywmbB612
Cucsa.357530	Wax gourd	cgywgoB712
Cucsa.357530	Cucurbita maxima (Rimu)	cgycmaB0971
Cucsa.357530	Cucurbita maxima (Rimu)	cgycmaB0972
Cucsa.357530	Cucurbita moschata (Rifu)	cgycmoB0975
Cucsa.357530	Melon (DHL92) v3.5.1	cgymeB576
Cucsa.357530	Watermelon (Charleston Gray)	cgywcgB608
Cucsa.357530	Watermelon (97103) v1	cgywmB643