CSPI04G01210 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G01210
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	RING/U-box superfamily protein
Location	Chr4 : 682182 .. 682790 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

mRNA sequence

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

Coding sequence (CDS)

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

Homology

[Close]

BLAST of CSPI04G01210 vs. Swiss-Prot
Match: RN181_MOUSE (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 8.7e-09
Identity = 29/64 (45.31%), Postives = 38/64 (59.38%), Query Frame = 1


Query: 135 VERLSKLKSEEEKGD--CSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 194
           VE L +      K D  C VCL E + E+  VI +PC H++H +CI  WL+ +NSCPLCR
Sbjct: 71  VESLPRTVISSAKADLKCPVCLLEFEAEET-VIEMPCHHLFHSNCILPWLSKTNSCPLCR 130

Query: 195 SSFP 197
              P
Sbjct: 131 HELP 133

[Close]

BLAST of CSPI04G01210 vs. Swiss-Prot
Match: RN181_RAT (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 25/56 (44.64%), Postives = 37/56 (66.07%), Query Frame = 1


Query: 141 LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 197
           ++S + +  C VCL E + E+  VI +PC H++H +CI  WL+ +NSCPLCR   P
Sbjct: 79  IRSSKAELKCPVCLLEFE-EEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELP 133

[Close]

BLAST of CSPI04G01210 vs. Swiss-Prot
Match: RN181_BOVIN (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus GN=RNF181 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 1


Query: 134 AVERLSK--LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLC 193
           AVE L +  ++  + +  C VCL E + E+   I +PC H++H +CI  WL+ +NSCPLC
Sbjct: 58  AVENLPRTVIRGSQAELKCPVCLLEFE-EEETAIEMPCHHLFHSNCILPWLSKTNSCPLC 117

Query: 194 RSSFPLHNVT 202
           R   P  + T
Sbjct: 118 RHELPTDDDT 126

[Close]

BLAST of CSPI04G01210 vs. Swiss-Prot
Match: RN181_XENTR (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 31/67 (46.27%), Postives = 40/67 (59.70%), Query Frame = 1


Query: 135 VERLSKLKSEEEKGD----CSVCLDELDCEKREVIR-IPCGHVYHESCIFKWLNSSNSCP 194
           VE L K+    E+ D    C VCL  L+ E+ E +R +PC H++H SCI  WL  +NSCP
Sbjct: 60  VESLPKVTVTPEQADAALKCPVCL--LEFEEGETVRQLPCEHLFHSSCILPWLGKTNSCP 119

Query: 195 LCRSSFP 197
           LCR   P
Sbjct: 120 LCRHELP 124

[Close]

BLAST of CSPI04G01210 vs. Swiss-Prot
Match: RN181_HUMAN (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens GN=RNF181 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.9e-08
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 1


Query: 135 VERLSK--LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 194
           VE L +  ++  + +  C VCL E + E+   I +PC H++H SCI  WL+ +NSCPLCR
Sbjct: 59  VENLPRTVIRGSQAELKCPVCLLEFE-EEETAIEMPCHHLFHSSCILPWLSKTNSCPLCR 118

Query: 195 SSFPLHNVT 202
              P  + T
Sbjct: 119 YELPTDDDT 126

[Close]

BLAST of CSPI04G01210 vs. TrEMBL
Match: A0A0A0KYC0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G003650 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.0e-113
Identity = 201/202 (99.50%), Postives = 202/202 (100.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS
Sbjct: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VHQLQHPLFHIRQFLYSLNIRRETIARQIAS+VAHMCNGNTGWNSNFYVIARVDLVRVIR
Sbjct: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASFVAHMCNGNTGWNSNFYVIARVDLVRVIR 120

Query: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180
           IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK
Sbjct: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180

Query: 181 WLNSSNSCPLCRSSFPLHNVTN 203
           WLNSSNSCPLCRSSFPLHNVTN
Sbjct: 181 WLNSSNSCPLCRSSFPLHNVTN 202

[Close]

BLAST of CSPI04G01210 vs. TrEMBL
Match: A0A0A0K2W2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-49
Identity = 116/225 (51.56%), Postives = 153/225 (68.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFG-NGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L A+G G NGVL+V +S++++ ISVS  + R   +L++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
           FYLS+ QLQ PLF+I Q L SLNIR    + +AR+IAS++  M + N+   +NF+++A V
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAEV 120

Query: 121 DLVRVIRI----------------EEQPRAEGWRGVAVERLSKLKSEE---EKGDCSVCL 180
           D +R+I +                +E P A   RGV V R  +L+SEE   E GDCS+CL
Sbjct: 121 DFIRLIWLPSMGGGGAGAEETVVLDEAPPAVK-RGVGVARGERLRSEEKMEELGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           DEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH+
Sbjct: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHH 220

[Close]

BLAST of CSPI04G01210 vs. TrEMBL
Match: A0A0A0K696_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-49
Identity = 116/225 (51.56%), Postives = 153/225 (68.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFG-NGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L A+G G NGVL+V +S++++ ISVS  + R   +L++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
           FYLS+ QLQ PLF+I Q L SLNIR    + +AR+IAS++  M + N+   +NF+++A V
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAEV 120

Query: 121 DLVRVIRI----------------EEQPRAEGWRGVAVERLSKLKSEE---EKGDCSVCL 180
           D +R+I +                +E P A   RGV V R  +L+SEE   E GDCS+CL
Sbjct: 121 DFIRLIWLPSMGGGGAGAEETVVLDEAPPAVK-RGVGVARGERLRSEEKMEELGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           DEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH+
Sbjct: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHH 220

[Close]

BLAST of CSPI04G01210 vs. TrEMBL
Match: A0A0A0LHI3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-21
Identity = 74/183 (40.44%), Postives = 99/183 (54.10%), Query Frame = 1


Query: 36  VSSFSQRILYETQPTPFNNALFYLSVHQLQ--HP-LFH--IRQFLYSLNIRRETIARQIA 95
           VS FS + + +T PT  +NALF+  + +L+  +P LFH  + QFL   +     I   I+
Sbjct: 36  VSPFSNQNIQQTFPTSISNALFHFPLPELELENPSLFHDFLLQFLSPAD--SAAIFHNIS 95

Query: 96  SYVAHMCNGNTGWNSNFYVIARVDLVRVIRIE--------EQPRAEGWRGVAVERLSKLK 155
           S+   +  GN   N NF++IA VDL+  I I           P   G     +ERL   K
Sbjct: 96  SFALQLAAGNL--NLNFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEK 155

Query: 156 SEEEKG------DCSVCLDELDCE----KREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 196
            ++  G       C VC ++L+CE    K E  RIPCGHVYH+SCI KWLN +NSCPLCR
Sbjct: 156 YDDGNGGEGMGGQCCVCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCR 214

[Close]

BLAST of CSPI04G01210 vs. TrEMBL
Match: A0A0A0LRB0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.4e-19
Identity = 75/206 (36.41%), Postives = 111/206 (53.88%), Query Frame = 1


Query: 14  NANGFG--NG----VLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLSVHQLQHP 73
           NANG    NG     +E++L +  +   V S S +IL +T P   ++ L    + +L+ P
Sbjct: 16  NANGSAATNGQPLQFVELRLGHFSQL--VLSPSHQILQQTSPILISDTLIRFPLRELEDP 75

Query: 74  LF----HIRQFLYSLNIRR---ETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 133
            F     + +FL S NI     + I  +I+S+   +  GN   NSNF++IA +D V    
Sbjct: 76  PFISHSSLCRFLSSYNISDAVCDAIFLKISSFAFQLVAGNL--NSNFHIIAALDFVYTHW 135

Query: 134 IEEQPRAE------GWRGVAVERLSKLK-----SEEEKGDCSVCLDELDCE---KREVIR 193
           ++  P  +      G    A+ERL K K      EE + +CSVC ++L  +   ++EV R
Sbjct: 136 VDLDPAEDAAAVRQGAPRSAIERLMKEKYDGNGGEEMEDECSVCYEDLHGKTEKEKEVSR 195

[Close]

BLAST of CSPI04G01210 vs. TAIR10
Match: AT5G37200.1 (AT5G37200.1 RING/U-box superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 7.5e-11
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1


Query: 131 RGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIP-CGHVYHESCIFKWLNSSNSCP 190
           R V  +R+     EEE   CS+C+++      ++IR+P C H++H+ C+F+WL   NSCP
Sbjct: 140 RLVEEQRVESADLEEEDETCSICIEKFSESHEDIIRVPDCLHLFHQGCLFEWLGLQNSCP 199

Query: 191 LCR 193
           LCR
Sbjct: 200 LCR 202

[Close]

BLAST of CSPI04G01210 vs. TAIR10
Match: AT4G12190.1 (AT4G12190.1 RING/U-box superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 26/63 (41.27%), Postives = 38/63 (60.32%), Query Frame = 1


Query: 133 VAVERLSKLKSEEEKGDCSVCLDELDC--EKREVIRIPCGHVYHESCIFKWLNSSNSCPL 192
           +  E L  LK E E   CS+CL+ L    + R++ R+ C HV+H  C+ +WL   N+CPL
Sbjct: 7   IKTEELKSLKMETEP--CSICLESLVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPL 66

Query: 193 CRS 194
           CR+
Sbjct: 67  CRT 67

[Close]

BLAST of CSPI04G01210 vs. TAIR10
Match: AT3G19950.1 (AT3G19950.1 RING/U-box superfamily protein)

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-09
Identity = 23/60 (38.33%), Postives = 38/60 (63.33%), Query Frame = 1


Query: 137 RLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 196
           +++K   + E   C+VC+DE + +  +V ++PC HV+H+ C+  WL   NSCP+CR   P
Sbjct: 203 KVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CSPI04G01210 vs. TAIR10
Match: AT5G37280.1 (AT5G37280.1 RING/U-box superfamily protein)

HSP 1 Score: 59.7 bits (143), Expect = 2.4e-09
Identity = 45/158 (28.48%), Postives = 72/158 (45.57%), Query Frame = 1


Query: 60  SVHQLQHPLFHIRQFL---YSLNIRR---------ETIARQIASYVAHMCNGNTGWNSNF 119
           S+  + H L   R FL    S  +RR         E++A +I+S      N  T +   F
Sbjct: 51  SLSPISHVLLKPRSFLPEQLSRLLRRQLHRDTSICESLAEKISSLRFSRAN-YTLYQRPF 110

Query: 120 YVIARVDLVRVIRIEEQPRAEGWRGVAVERLSKLKSEE-------EKGDCSVCLDELDCE 179
            +  +V +++ +R    P +    G  V+   +L  E+           CS+C ++L   
Sbjct: 111 LMTVKVRVIKEVRFIVSPVSAPSSGAPVDVFQRLLEEQTVEPSMDSDESCSICFEKLSDS 170

Query: 180 KRE-----VIRIP-CGHVYHESCIFKWLNSSNSCPLCR 193
             E     +I++P C H +H+ CIFKW+   NSCPLCR
Sbjct: 171 LSETYHNSIIQMPKCLHSFHQKCIFKWIGRQNSCPLCR 207

[Close]

BLAST of CSPI04G01210 vs. TAIR10
Match: AT1G35330.1 (AT1G35330.1 RING/U-box superfamily protein)

HSP 1 Score: 59.3 bits (142), Expect = 3.2e-09
Identity = 21/48 (43.75%), Postives = 34/48 (70.83%), Query Frame = 1


Query: 149 DCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 197
           +C++CL+E + E+   +  PC H +H SCI  WL+S ++CP+CR+S P
Sbjct: 127 ECAICLNEFEDEETLRLMPPCSHAFHASCIDVWLSSRSTCPVCRASLP 174

[Close]

BLAST of CSPI04G01210 vs. NCBI nr
Match: gi|778697402|ref|XP_011654314.1| (PREDICTED: E3 ubiquitin-protein ligase RNF115-like [Cucumis sativus])

HSP 1 Score: 416.4 bits (1069), Expect = 2.9e-113
Identity = 201/202 (99.50%), Postives = 202/202 (100.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS
Sbjct: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VHQLQHPLFHIRQFLYSLNIRRETIARQIAS+VAHMCNGNTGWNSNFYVIARVDLVRVIR
Sbjct: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASFVAHMCNGNTGWNSNFYVIARVDLVRVIR 120

Query: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180
           IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK
Sbjct: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180

Query: 181 WLNSSNSCPLCRSSFPLHNVTN 203
           WLNSSNSCPLCRSSFPLHNVTN
Sbjct: 181 WLNSSNSCPLCRSSFPLHNVTN 202

[Close]

BLAST of CSPI04G01210 vs. NCBI nr
Match: gi|659108614|ref|XP_008454295.1| (PREDICTED: uncharacterized protein LOC103494735 isoform X1 [Cucumis melo])

HSP 1 Score: 217.6 bits (553), Expect = 2.0e-53
Identity = 123/211 (58.29%), Postives = 141/211 (66.82%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSF ISSPI YLLN  GFGNGVLE+QLSY+ER IS+SS SQRILYETQP PFNNA FYLS
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVSQRILYETQPIPFNNAHFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VH LQ PLF+IRQFL+SLNIRRE IARQIASYVAHMCNGNTG NSNFYVIARVDLVR IR
Sbjct: 61  VHHLQDPLFYIRQFLFSLNIRRENIARQIASYVAHMCNGNTGRNSNFYVIARVDLVRAIR 120

Query: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGD-------CSVCLDELDCEKREVIRIPCGHVY 180
           IEE+       GV VE  + +  E  +           VC+  +          P     
Sbjct: 121 IEEESMWRDGGGVVVEAETVVFDEVPRARRGVVCVFVCVCVCVILISHYSFTLQPKQQQQ 180

Query: 181 HESCIFK--WLNSSNSCPLCRSSFPLHNVTN 203
            +  +F   +L  S+S P+  S FP+ N T+
Sbjct: 181 QQKAVFSIPFLFLSSSSPIPNSQFPIPNSTS 211

[Close]

BLAST of CSPI04G01210 vs. NCBI nr
Match: gi|449454422|ref|XP_004144954.1| (PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 5.0e-49
Identity = 116/225 (51.56%), Postives = 153/225 (68.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFG-NGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L A+G G NGVL+V +S++++ ISVS  + R   +L++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
           FYLS+ QLQ PLF+I Q L SLNIR    + +AR+IAS++  M + N+   +NF+++A V
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAEV 120

Query: 121 DLVRVIRI----------------EEQPRAEGWRGVAVERLSKLKSEE---EKGDCSVCL 180
           D +R+I +                +E P A   RGV V R  +L+SEE   E GDCS+CL
Sbjct: 121 DFIRLIWLPSMGGGGAGAEETVVLDEAPPAVK-RGVGVARGERLRSEEKMEELGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           DEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH+
Sbjct: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHH 220

[Close]

BLAST of CSPI04G01210 vs. NCBI nr
Match: gi|778730084|ref|XP_011659694.1| (PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 5.0e-49
Identity = 116/225 (51.56%), Postives = 153/225 (68.00%), Query Frame = 1


Query: 1   MSFEISSPIPYLLNANGFG-NGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L A+G G NGVL+V +S++++ ISVS  + R   +L++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
           FYLS+ QLQ PLF+I Q L SLNIR    + +AR+IAS++  M + N+   +NF+++A V
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAEV 120

Query: 121 DLVRVIRI----------------EEQPRAEGWRGVAVERLSKLKSEE---EKGDCSVCL 180
           D +R+I +                +E P A   RGV V R  +L+SEE   E GDCS+CL
Sbjct: 121 DFIRLIWLPSMGGGGAGAEETVVLDEAPPAVK-RGVGVARGERLRSEEKMEELGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           DEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH+
Sbjct: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHH 220

[Close]

BLAST of CSPI04G01210 vs. NCBI nr
Match: gi|778689036|ref|XP_011652885.1| (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus])

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-21
Identity = 74/183 (40.44%), Postives = 99/183 (54.10%), Query Frame = 1


Query: 36  VSSFSQRILYETQPTPFNNALFYLSVHQLQ--HP-LFH--IRQFLYSLNIRRETIARQIA 95
           VS FS + + +T PT  +NALF+  + +L+  +P LFH  + QFL   +     I   I+
Sbjct: 36  VSPFSNQNIQQTFPTSISNALFHFPLPELELENPSLFHDFLLQFLSPAD--SAAIFHNIS 95

Query: 96  SYVAHMCNGNTGWNSNFYVIARVDLVRVIRIE--------EQPRAEGWRGVAVERLSKLK 155
           S+   +  GN   N NF++IA VDL+  I I           P   G     +ERL   K
Sbjct: 96  SFALQLAAGNL--NLNFHLIATVDLLYTISINLNLNPPQYSPPVRNGVPASVMERLMNEK 155

Query: 156 SEEEKG------DCSVCLDELDCE----KREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 196
            ++  G       C VC ++L+CE    K E  RIPCGHVYH+SCI KWLN +NSCPLCR
Sbjct: 156 YDDGNGGEGMGGQCCVCYEDLNCEREEEKEEATRIPCGHVYHKSCILKWLNVNNSCPLCR 214

The following BLAST results are available for this feature:

BLAST of CSPI04G01210 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RN181_MOUSE	8.7e-09	45.31	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus GN=Rnf181 PE=1 SV=1	[more]
RN181_RAT	1.1e-08	44.64	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus GN=Rnf181 PE=1 SV=1	[more]
RN181_BOVIN	1.1e-08	40.00	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus GN=RNF181 PE=2 SV=1	[more]
RN181_XENTR	1.1e-08	46.27	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis GN=rnf181 PE=2 SV=1	[more]
RN181_HUMAN	1.9e-08	40.58	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens GN=RNF181 PE=1 SV=1	[more]

BLAST of CSPI04G01210 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYC0_CUCSA	2.0e-113	99.50	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G003650 PE=4 SV=1	[more]
A0A0A0K2W2_CUCSA	3.5e-49	51.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G025210 PE=4 SV=1	[more]
A0A0A0K696_CUCSA	3.5e-49	51.56	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G026750 PE=4 SV=1	[more]
A0A0A0LHI3_CUCSA	1.1e-21	40.44	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G889100 PE=4 SV=1	[more]
A0A0A0LRB0_CUCSA	6.4e-19	36.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G432790 PE=4 SV=1	[more]

BLAST of CSPI04G01210 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G37200.1	7.5e-11	41.27	RING/U-box superfamily protein	[more]
AT4G12190.1	1.1e-09	41.27	RING/U-box superfamily protein	[more]
AT3G19950.1	1.9e-09	38.33	RING/U-box superfamily protein	[more]
AT5G37280.1	2.4e-09	28.48	RING/U-box superfamily protein	[more]
AT1G35330.1	3.2e-09	43.75	RING/U-box superfamily protein	[more]

BLAST of CSPI04G01210 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778697402\|ref\|XP_011654314.1\|	2.9e-113	99.50	PREDICTED: E3 ubiquitin-protein ligase RNF115-like [Cucumis sativus]	[more]
gi\|659108614\|ref\|XP_008454295.1\|	2.0e-53	58.29	PREDICTED: uncharacterized protein LOC103494735 isoform X1 [Cucumis melo]	[more]
gi\|449454422\|ref\|XP_004144954.1\|	5.0e-49	51.56	PREDICTED: uncharacterized protein LOC101214496 [Cucumis sativus]	[more]
gi\|778730084\|ref\|XP_011659694.1\|	5.0e-49	51.56	PREDICTED: uncharacterized protein LOC105436225 [Cucumis sativus]	[more]
gi\|778689036\|ref\|XP_011652885.1\|	1.5e-21	40.44	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001841	Znf_RING
IPR013083	Znf_RING/FYVE/PHD

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding
GO:0008270	zinc ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G01210.1	CSPI04G01210.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 149..192 score: 1.5
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 150..191 score: 4.
IPR001841	Zinc finger, RING-type	PROFILE	PS50089	ZF_RING_2	coord: 150..192 score: 13
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	G3DSA:3.30.40.10		coord: 141..199 score: 1.3
None	No IPR available	PANTHER	PTHR22763	RING ZINC FINGER PROTEIN	coord: 30..65 score: 8.7E-15coord: 116..201 score: 8.7
None	No IPR available	PANTHER	PTHR22763:SF46	PROTEIN SAN1	coord: 116..201 score: 8.7E-15coord: 30..65 score: 8.7
None	No IPR available	unknown	SSF57850	RING/U-box	coord: 149..197 score: 5.89

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G01210	Csa4G003650	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G01210	MELO3C017919	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G01210	MELO3C017919.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G01210	CsaV3_4G001200	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G01210	CsGy4G001100	Cucumber (Gy14) v2	cgybcpiB168

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G01210	Cucurbita pepo (Zucchini)	cpecpiB141
CSPI04G01210	Cucurbita pepo (Zucchini)	cpecpiB316
CSPI04G01210	Cucurbita pepo (Zucchini)	cpecpiB568
CSPI04G01210	Cucurbita pepo (Zucchini)	cpecpiB618
CSPI04G01210	Bottle gourd (USVL1VR-Ls)	cpilsiB266
CSPI04G01210	Bottle gourd (USVL1VR-Ls)	cpilsiB274
CSPI04G01210	Melon (DHL92) v3.6.1	cpimedB269
CSPI04G01210	Cucumber (Gy14) v2	cgybcpiB322
CSPI04G01210	Silver-seed gourd	carcpiB0121
CSPI04G01210	Silver-seed gourd	carcpiB0800
CSPI04G01210	Silver-seed gourd	carcpiB0854
CSPI04G01210	Cucumber (Chinese Long) v3	cpicucB246
CSPI04G01210	Watermelon (97103) v2	cpiwmbB278
CSPI04G01210	Watermelon (97103) v2	cpiwmbB346
CSPI04G01210	Wax gourd	cpiwgoB358
CSPI04G01210	Wild cucumber (PI 183967)	cpicpiB167
CSPI04G01210	Cucumber (Gy14) v1	cgycpiB103
CSPI04G01210	Cucumber (Gy14) v1	cgycpiB324
CSPI04G01210	Cucurbita maxima (Rimu)	cmacpiB264
CSPI04G01210	Cucurbita maxima (Rimu)	cmacpiB369
CSPI04G01210	Cucurbita maxima (Rimu)	cmacpiB478
CSPI04G01210	Cucurbita maxima (Rimu)	cmacpiB913
CSPI04G01210	Cucurbita moschata (Rifu)	cmocpiB251
CSPI04G01210	Cucurbita moschata (Rifu)	cmocpiB359
CSPI04G01210	Cucurbita moschata (Rifu)	cmocpiB468
CSPI04G01210	Cucurbita moschata (Rifu)	cmocpiB892
CSPI04G01210	Cucumber (Chinese Long) v2	cpicuB205
CSPI04G01210	Melon (DHL92) v3.5.1	cpimeB274
CSPI04G01210	Watermelon (Charleston Gray)	cpiwcgB278
CSPI04G01210	Watermelon (Charleston Gray)	cpiwcgB280
CSPI04G01210	Watermelon (97103) v1	cpiwmB288
CSPI04G01210	Watermelon (97103) v1	cpiwmB291