CSPI04G00690 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G00690
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	rapid alkalinization factor 1
Location	Chr4 : 354774 .. 355215 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCTCTTTCCCTTGGTAGACGTAGAATTCACTAAAGTAATAAGAAAGAAGTGAAGGCAAACCATGGGCAGCTCCTCTTCTCCACCTGCCTTATCTATTCTCATTTTCACCATAGCCTTCTTCGTCTCGTCGTCGTCCTCCTCCTTAGTAGTCACGACCATGAGCAGCGATCGGAGTCTCAATTGGCTCTCGACGGAAGCACGATGCCATGGACGTTCAATAAGCGAATGCATGATGCACATTGAGTTTGAAATGGATTCTGAGATCAATCGTCGCATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAGGGCAAACAACATTCCATGCTCTCGAAGAGGTTCGTCCTACTACAATTGCCAACCCGGAGCCGAAGCTAACCCATATCAGCGGGGTTGTACTGCCATTACTCGTTGCAGAAGCTAAGAAAAACTCT

mRNA sequence

ATGGGCAGCTCCTCTTCTCCACCTGCCTTATCTATTCTCATTTTCACCATAGCCTTCTTCGTCTCGTCGTCGTCCTCCTCCTTAGTAGTCACGACCATGAGCAGCGATCGGAGTCTCAATTGGCTCTCGACGGAAGCACGATGCCATGGACGTTCAATAAGCGAATGCATGATGCACATTGAGTTTGAAATGGATTCTGAGATCAATCGTCGCATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAGGGCAAACAACATTCCATGCTCTCGAAGAGGTTCGTCCTACTACAATTGCCAACCCGGAGCCGAAGCTAACCCATATCAGCGGGGTTGTACTGCCATTACTCGTTGCAGAAGCTAA

Coding sequence (CDS)

ATGGGCAGCTCCTCTTCTCCACCTGCCTTATCTATTCTCATTTTCACCATAGCCTTCTTCGTCTCGTCGTCGTCCTCCTCCTTAGTAGTCACGACCATGAGCAGCGATCGGAGTCTCAATTGGCTCTCGACGGAAGCACGATGCCATGGACGTTCAATAAGCGAATGCATGATGCACATTGAGTTTGAAATGGATTCTGAGATCAATCGTCGCATCTTAGCCACTTCAAGTTACATAAGTTACAAGTCGTTGAGGGCAAACAACATTCCATGCTCTCGAAGAGGTTCGTCCTACTACAATTGCCAACCCGGAGCCGAAGCTAACCCATATCAGCGGGGTTGTACTGCCATTACTCGTTGCAGAAGCTAA

Homology

[Close]

BLAST of CSPI04G00690 vs. Swiss-Prot
Match: RLF33_ARATH (Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 8.1e-26
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 1


Query: 2   GSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI- 61
           G S+ P A+ I I T+ F  ++      VT+ SS    +++  E++C+G +I+EC +   
Sbjct: 3   GLSTKPVAIIIAILTVHFLFAA------VTSQSSG---DFVPIESKCNG-TIAECSLSTA 62

Query: 62  --EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAIT 121
             EFEMDSEINRRILAT+ YISY +LR N +PCSRRG+SYYNC+ GA+ANPY RGC+AIT
Sbjct: 63  EEEFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAIT 115

[Close]

BLAST of CSPI04G00690 vs. Swiss-Prot
Match: RLF1_ARATH (Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 6.8e-25
Identity = 54/75 (72.00%), Postives = 64/75 (85.33%), Query Frame = 1


Query: 48  CHGRSISECMMHIEFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEA 107
           CHG SI+EC+   E EMDSEINRRILAT+ YISY+SL+ N++PCSRRG+SYYNCQ GA+A
Sbjct: 47  CHG-SIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 108 NPYQRGCTAITRCRS 123
           NPY RGC+ I RCRS
Sbjct: 107 NPYSRGCSKIARCRS 120

[Close]

BLAST of CSPI04G00690 vs. Swiss-Prot
Match: RALF_TOBAC (Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 6.4e-23
Identity = 60/120 (50.00%), Postives = 80/120 (66.67%), Query Frame = 1


Query: 7   PPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLS---TEARCHGRSISECMMHIE-F 66
           P  L + +   AFF+S +++           + +W+    +   C G SI EC+   E F
Sbjct: 4   PSGLILCVLIGAFFISMAAAG-------DSGAYDWVMPARSGGGCKG-SIGECIAEEEEF 63

Query: 67  EMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           E+DSE NRRILAT  YISY +L+ N++PCSRRG+SYYNC+PGA+ANPY RGC+AITRCRS
Sbjct: 64  ELDSESNRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of CSPI04G00690 vs. Swiss-Prot
Match: RLF23_ARATH (Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 8.3e-23
Identity = 62/125 (49.60%), Postives = 80/125 (64.00%), Query Frame = 1


Query: 14  IFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECM---------------- 73
           IF I   ++  + S+ V++ S++ + ++   E  C G +I+EC                 
Sbjct: 13  IFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRG-TIAECSVSAALGDGGDLFYGGG 72

Query: 74  -MHIEFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTA 122
            M  EFEMDSEINRRILAT  YISY +LR N IPCSRRG+SYYNC+ GA+ANPY RGC+A
Sbjct: 73  EMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSA 132

[Close]

BLAST of CSPI04G00690 vs. Swiss-Prot
Match: RLF22_ARATH (Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.7e-20
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 1


Query: 48  CHGRSISECMMHIE-FEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAE 107
           C G SI+EC+   E  E DS+I+RRILA   YISY ++R N++PCSRRG+SYYNCQ GA+
Sbjct: 45  CTG-SIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 108 ANPYQRGCTAITRCR 122
           ANPY RGC+ ITRCR
Sbjct: 105 ANPYSRGCSTITRCR 118

[Close]

BLAST of CSPI04G00690 vs. TrEMBL
Match: A0A0A0KWI2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001640 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 5.9e-60
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1


Query: 1   MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI 60
           MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI
Sbjct: 1   MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI 60

Query: 61  EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC 120
           EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC
Sbjct: 61  EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC 120

Query: 121 RS 123
           RS
Sbjct: 121 RS 122

[Close]

BLAST of CSPI04G00690 vs. TrEMBL
Match: B9T0V9_RICCO (RALFL33, putative OS=Ricinus communis GN=RCOM_1439830 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.7e-28
Identity = 66/112 (58.93%), Postives = 83/112 (74.11%), Query Frame = 1


Query: 11  SILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINR 70
           SI +FT    +SS   S      + D  LNW+   +RC G ++++CM   EF+MDSEINR
Sbjct: 6   SIFLFTFYLLLSSLIISTSTVEATIDHKLNWVPKTSRCQG-TLADCMQEDEFDMDSEINR 65

Query: 71  RILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           RILAT++YISY +L+ N IPCS+RG+SYYNCQPGAEANPY RGC+AITRCRS
Sbjct: 66  RILATTNYISYGALQRNTIPCSQRGASYYNCQPGAEANPYSRGCSAITRCRS 116

[Close]

BLAST of CSPI04G00690 vs. TrEMBL
Match: A0A0A0LFW9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G031140 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 4.6e-28
Identity = 68/110 (61.82%), Postives = 79/110 (71.82%), Query Frame = 1


Query: 13  LIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINRRI 72
           LI    F + S SS+ V   +    SL W+  ++ C G SI+EC    EFE DSEINRRI
Sbjct: 13  LICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKG-SIAECFGGEEFEFDSEINRRI 72

Query: 73  LATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           LATS YISY +LR NN+PCSRRG+SYYNCQPGA+ANPY RGC AITRCRS
Sbjct: 73  LATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 121

[Close]

BLAST of CSPI04G00690 vs. TrEMBL
Match: B9I9T7_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12630g PE=4 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 3.9e-27
Identity = 68/114 (59.65%), Postives = 86/114 (75.44%), Query Frame = 1


Query: 10  LSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEAR-CHGRSISECMMHIEFEMDSEI 69
           L +++   A  +SSS+        S D +LNW+ T AR C G +++ECM + EFEMDSEI
Sbjct: 8   LFLVLLLSALIISSST-----VDASEDHNLNWVPTRARGCQG-TVAECMGNDEFEMDSEI 67

Query: 70  NRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           NRRILATS+YISY +L  NN+PCS+RG+SYYNC+ GAEANPY RGC+AITRCRS
Sbjct: 68  NRRILATSNYISYDALGKNNVPCSQRGASYYNCKTGAEANPYSRGCSAITRCRS 115

[Close]

BLAST of CSPI04G00690 vs. TrEMBL
Match: M5VTQ6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020353mg PE=4 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.1e-26
Identity = 61/102 (59.80%), Postives = 80/102 (78.43%), Query Frame = 1


Query: 25  SSSLVVTTMS----SDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINRRILATSSYIS 84
           +++L+++T +    SD  L+W+    RC G SI+ECM   EF+MDS+INRRILATS YIS
Sbjct: 3   TATLIISTWTVHAGSDHGLSWVPGRTRCQG-SIAECMADDEFDMDSQINRRILATSQYIS 62

Query: 85  YKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           Y +L+ N +PCSRRG+SYYNC+PGAE+NPY RGC+AI RCRS
Sbjct: 63  YGALQRNTVPCSRRGASYYNCKPGAESNPYNRGCSAIARCRS 103

[Close]

BLAST of CSPI04G00690 vs. TAIR10
Match: AT4G15800.1 (AT4G15800.1 ralf-like 33)

HSP 1 Score: 117.9 bits (294), Expect = 4.5e-27
Identity = 66/123 (53.66%), Postives = 88/123 (71.54%), Query Frame = 1


Query: 2   GSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI- 61
           G S+ P A+ I I T+ F  ++      VT+ SS    +++  E++C+G +I+EC +   
Sbjct: 3   GLSTKPVAIIIAILTVHFLFAA------VTSQSSG---DFVPIESKCNG-TIAECSLSTA 62

Query: 62  --EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAIT 121
             EFEMDSEINRRILAT+ YISY +LR N +PCSRRG+SYYNC+ GA+ANPY RGC+AIT
Sbjct: 63  EEEFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAIT 115

[Close]

BLAST of CSPI04G00690 vs. TAIR10
Match: AT1G02900.1 (AT1G02900.1 rapid alkalinization factor 1)

HSP 1 Score: 114.8 bits (286), Expect = 3.8e-26
Identity = 54/75 (72.00%), Postives = 64/75 (85.33%), Query Frame = 1


Query: 48  CHGRSISECMMHIEFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEA 107
           CHG SI+EC+   E EMDSEINRRILAT+ YISY+SL+ N++PCSRRG+SYYNCQ GA+A
Sbjct: 47  CHG-SIAECIGAEEEEMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQA 106

Query: 108 NPYQRGCTAITRCRS 123
           NPY RGC+ I RCRS
Sbjct: 107 NPYSRGCSKIARCRS 120

[Close]

BLAST of CSPI04G00690 vs. TAIR10
Match: AT3G16570.1 (AT3G16570.1 rapid alkalinization factor 23)

HSP 1 Score: 107.8 bits (268), Expect = 4.7e-24
Identity = 62/125 (49.60%), Postives = 80/125 (64.00%), Query Frame = 1


Query: 14  IFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECM---------------- 73
           IF I   ++  + S+ V++ S++ + ++   E  C G +I+EC                 
Sbjct: 13  IFAILLILAVHNWSVAVSSQSTEFAGDFPPFETECRG-TIAECSVSAALGDGGDLFYGGG 72

Query: 74  -MHIEFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTA 122
            M  EFEMDSEINRRILAT  YISY +LR N IPCSRRG+SYYNC+ GA+ANPY RGC+A
Sbjct: 73  EMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRGAQANPYSRGCSA 132

[Close]

BLAST of CSPI04G00690 vs. TAIR10
Match: AT3G05490.1 (AT3G05490.1 ralf-like 22)

HSP 1 Score: 100.1 bits (248), Expect = 9.8e-22
Identity = 48/75 (64.00%), Postives = 59/75 (78.67%), Query Frame = 1


Query: 48  CHGRSISECMMHIE-FEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAE 107
           C G SI+EC+   E  E DS+I+RRILA   YISY ++R N++PCSRRG+SYYNCQ GA+
Sbjct: 45  CTG-SIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQ 104

Query: 108 ANPYQRGCTAITRCR 122
           ANPY RGC+ ITRCR
Sbjct: 105 ANPYSRGCSTITRCR 118

[Close]

BLAST of CSPI04G00690 vs. TAIR10
Match: AT2G33775.1 (AT2G33775.1 ralf-like 19)

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-16
Identity = 42/94 (44.68%), Postives = 59/94 (62.77%), Query Frame = 1


Query: 28  LVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINRRILATS-SYISYKSLRA 87
           L +  ++   +  W  T++  +G+        +++ MDSE NRR LA   SYISY +LR 
Sbjct: 12  LTLAVVAESANATWTLTKSCVNGQGCIGEDGELDYLMDSETNRRQLAARRSYISYGALRK 71

Query: 88  NNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC 121
           NN+PCSRRG SYY+C+    ANPY+RGC+ IT C
Sbjct: 72  NNVPCSRRGRSYYDCKKRKRANPYRRGCSVITHC 105

[Close]

BLAST of CSPI04G00690 vs. NCBI nr
Match: gi|700197639|gb|KGN52797.1| (hypothetical protein Csa_4G001640 [Cucumis sativus])

HSP 1 Score: 238.0 bits (606), Expect = 8.5e-60
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 1


Query: 1   MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI 60
           MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI
Sbjct: 1   MGSSSSPPALSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHI 60

Query: 61  EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC 120
           EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC
Sbjct: 61  EFEMDSEINRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRC 120

Query: 121 RS 123
           RS
Sbjct: 121 RS 122

[Close]

BLAST of CSPI04G00690 vs. NCBI nr
Match: gi|255582168|ref|XP_002531878.1| (PREDICTED: protein RALF-like 33 [Ricinus communis])

HSP 1 Score: 132.9 bits (333), Expect = 3.9e-28
Identity = 66/112 (58.93%), Postives = 83/112 (74.11%), Query Frame = 1


Query: 11  SILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINR 70
           SI +FT    +SS   S      + D  LNW+   +RC G ++++CM   EF+MDSEINR
Sbjct: 6   SIFLFTFYLLLSSLIISTSTVEATIDHKLNWVPKTSRCQG-TLADCMQEDEFDMDSEINR 65

Query: 71  RILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           RILAT++YISY +L+ N IPCS+RG+SYYNCQPGAEANPY RGC+AITRCRS
Sbjct: 66  RILATTNYISYGALQRNTIPCSQRGASYYNCQPGAEANPYSRGCSAITRCRS 116

[Close]

BLAST of CSPI04G00690 vs. NCBI nr
Match: gi|700205836|gb|KGN60955.1| (hypothetical protein Csa_2G031140 [Cucumis sativus])

HSP 1 Score: 132.1 bits (331), Expect = 6.6e-28
Identity = 68/110 (61.82%), Postives = 79/110 (71.82%), Query Frame = 1


Query: 13  LIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINRRI 72
           LI    F + S SS+ V   +    SL W+  ++ C G SI+EC    EFE DSEINRRI
Sbjct: 13  LICAAVFLIFSCSSTTVHAGLGIQHSLAWIPNQSTCKG-SIAECFGGEEFEFDSEINRRI 72

Query: 73  LATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           LATS YISY +LR NN+PCSRRG+SYYNCQPGA+ANPY RGC AITRCRS
Sbjct: 73  LATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 121

[Close]

BLAST of CSPI04G00690 vs. NCBI nr
Match: gi|659070254|ref|XP_008454184.1| (PREDICTED: protein RALF-like 33 [Cucumis melo])

HSP 1 Score: 131.7 bits (330), Expect = 8.6e-28
Identity = 68/110 (61.82%), Postives = 79/110 (71.82%), Query Frame = 1


Query: 13  LIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEARCHGRSISECMMHIEFEMDSEINRRI 72
           LI    F + S SS+ V   +    SL W+  ++ C G SI+EC    EFE DSEINRRI
Sbjct: 13  LISAAVFLIFSCSSTAVHAGIGIQNSLAWIPNQSTCKG-SIAECFGGEEFEFDSEINRRI 72

Query: 73  LATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           LATS YISY +LR NN+PCSRRG+SYYNCQPGA+ANPY RGC AITRCRS
Sbjct: 73  LATSQYISYGALRRNNVPCSRRGASYYNCQPGAQANPYSRGCNAITRCRS 121

[Close]

BLAST of CSPI04G00690 vs. NCBI nr
Match: gi|566203975|ref|XP_002321018.2| (hypothetical protein POPTR_0014s12630g [Populus trichocarpa])

HSP 1 Score: 129.0 bits (323), Expect = 5.6e-27
Identity = 68/114 (59.65%), Postives = 86/114 (75.44%), Query Frame = 1


Query: 10  LSILIFTIAFFVSSSSSSLVVTTMSSDRSLNWLSTEAR-CHGRSISECMMHIEFEMDSEI 69
           L +++   A  +SSS+        S D +LNW+ T AR C G +++ECM + EFEMDSEI
Sbjct: 8   LFLVLLLSALIISSST-----VDASEDHNLNWVPTRARGCQG-TVAECMGNDEFEMDSEI 67

Query: 70  NRRILATSSYISYKSLRANNIPCSRRGSSYYNCQPGAEANPYQRGCTAITRCRS 123
           NRRILATS+YISY +L  NN+PCS+RG+SYYNC+ GAEANPY RGC+AITRCRS
Sbjct: 68  NRRILATSNYISYDALGKNNVPCSQRGASYYNCKTGAEANPYSRGCSAITRCRS 115

The following BLAST results are available for this feature:

BLAST of CSPI04G00690 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF33_ARATH	8.1e-26	53.66	Protein RALF-like 33 OS=Arabidopsis thaliana GN=RALFL33 PE=2 SV=1	[more]
RLF1_ARATH	6.8e-25	72.00	Protein RALF-like 1 OS=Arabidopsis thaliana GN=RALF1 PE=1 SV=1	[more]
RALF_TOBAC	6.4e-23	50.00	Rapid alkalinization factor OS=Nicotiana tabacum GN=RALF PE=1 SV=1	[more]
RLF23_ARATH	8.3e-23	49.60	Rapid alkalinization factor 23 OS=Arabidopsis thaliana GN=RALF23 PE=1 SV=1	[more]
RLF22_ARATH	1.7e-20	64.00	Protein RALF-like 22 OS=Arabidopsis thaliana GN=RALFL22 PE=3 SV=1	[more]

BLAST of CSPI04G00690 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWI2_CUCSA	5.9e-60	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001640 PE=4 SV=1	[more]
B9T0V9_RICCO	2.7e-28	58.93	RALFL33, putative OS=Ricinus communis GN=RCOM_1439830 PE=4 SV=1	[more]
A0A0A0LFW9_CUCSA	4.6e-28	61.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G031140 PE=4 SV=1	[more]
B9I9T7_POPTR	3.9e-27	59.65	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0014s12630g PE=4 SV=2	[more]
M5VTQ6_PRUPE	1.1e-26	59.80	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020353mg PE=4 SV=1	[more]

BLAST of CSPI04G00690 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G15800.1	4.5e-27	53.66	ralf-like 33	[more]
AT1G02900.1	3.8e-26	72.00	rapid alkalinization factor 1	[more]
AT3G16570.1	4.7e-24	49.60	rapid alkalinization factor 23	[more]
AT3G05490.1	9.8e-22	64.00	ralf-like 22	[more]
AT2G33775.1	1.2e-16	44.68	ralf-like 19	[more]

BLAST of CSPI04G00690 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197639\|gb\|KGN52797.1\|	8.5e-60	100.00	hypothetical protein Csa_4G001640 [Cucumis sativus]	[more]
gi\|255582168\|ref\|XP_002531878.1\|	3.9e-28	58.93	PREDICTED: protein RALF-like 33 [Ricinus communis]	[more]
gi\|700205836\|gb\|KGN60955.1\|	6.6e-28	61.82	hypothetical protein Csa_2G031140 [Cucumis sativus]	[more]
gi\|659070254\|ref\|XP_008454184.1\|	8.6e-28	61.82	PREDICTED: protein RALF-like 33 [Cucumis melo]	[more]
gi\|566203975\|ref\|XP_002321018.2\|	5.6e-27	59.65	hypothetical protein POPTR_0014s12630g [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
biological_process	GO:0007267	cell-cell signaling
biological_process	GO:0008150	biological_process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005622	intracellular
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005179	hormone activity
molecular_function	GO:0004871	signal transducer activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00690.1	CSPI04G00690.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 61..121 score: 2.7
None	No IPR available	PANTHER	PTHR33136	FAMILY NOT NAMED	coord: 12..122 score: 7.5
None	No IPR available	PANTHER	PTHR33136:SF5	PROTEIN RALF-LIKE 1-RELATED	coord: 12..122 score: 7.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G00690	Cla001316	Watermelon (97103) v1	cpiwmB291
CSPI04G00690	Csa4G001640	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G00690	MELO3C017971	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G00690	ClCG10G010660	Watermelon (Charleston Gray)	cpiwcgB287
CSPI04G00690	Lsi02G023850	Bottle gourd (USVL1VR-Ls)	cpilsiB266
CSPI04G00690	Lsi03G010550	Bottle gourd (USVL1VR-Ls)	cpilsiB274
CSPI04G00690	MELO3C017971.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G00690	CsaV3_4G000650	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G00690	Cla97C10G193640	Watermelon (97103) v2	cpiwmbB278
CSPI04G00690	Cucsa.205730	Cucumber (Gy14) v1	cgycpiB324
CSPI04G00690	Cucsa.097250	Cucumber (Gy14) v1	cgycpiB103
CSPI04G00690	CmaCh14G008930	Cucurbita maxima (Rimu)	cmacpiB264
CSPI04G00690	CmaCh17G011040	Cucurbita maxima (Rimu)	cmacpiB369
CSPI04G00690	CmoCh17G010790	Cucurbita moschata (Rifu)	cmocpiB359
CSPI04G00690	CmoCh14G009220	Cucurbita moschata (Rifu)	cmocpiB251
CSPI04G00690	Cp4.1LG12g05450	Cucurbita pepo (Zucchini)	cpecpiB141
CSPI04G00690	Carg07186	Silver-seed gourd	carcpiB0121
CSPI04G00690	Carg11125	Silver-seed gourd	carcpiB0854

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CSPI04G00690	CSPI07G17290	Wild cucumber (PI 183967)	cpicpiB167

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G00690	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G00690	Silver-seed gourd	carcpiB0800
CSPI04G00690	Cucumber (Chinese Long) v3	cpicucB246
CSPI04G00690	Wax gourd	cpiwgoB358
CSPI04G00690	Cucurbita maxima (Rimu)	cmacpiB478
CSPI04G00690	Cucurbita maxima (Rimu)	cmacpiB913
CSPI04G00690	Cucurbita moschata (Rifu)	cmocpiB468
CSPI04G00690	Cucurbita moschata (Rifu)	cmocpiB892
CSPI04G00690	Watermelon (Charleston Gray)	cpiwcgB280
CSPI04G00690	Cucurbita pepo (Zucchini)	cpecpiB316
CSPI04G00690	Cucurbita pepo (Zucchini)	cpecpiB568
CSPI04G00690	Cucurbita pepo (Zucchini)	cpecpiB618