CSPI03G27710 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI03G27710
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Descriptionnuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein
LocationChr3 : 25225583 .. 25227221 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAAAAAGAATTAATTGGATTCTCGAACCAAGAAAAAGAAAAGAAGAGGTGACACGTTGAAGGTAGTCATGGGAGAGAGAGAAGAGAAAGGGATTTGAAAGAAGAAAAAGGAGATAGATTAATGGCGTACGCGCAGGCTTCGGTTTCGCTAAAAATATCTTTGCTTCATCTTCTTCATTCCTACGTGTTTCCTTCTTAACCCCACCGCCCCTTTCTTCTTCTCTCTCATCGCCACGTCGCCAATTCTCCTTTTCCCACAATCACTCCATCCTTATATTCCAAACCCACCAATCTTCCTCCTCCTCCTCCGAATCCATTCTTTCACAGATGAAACATCAACAAAACCTCGATTTACGCCTTTCAATCGGCGGACCTTTCACTAGTTTACCGGAAAAGCTAAATGGGTCTGATCAAGAAAGCGGAGTTACGGATGTCAACAACAGAGTCGACATCCGCCGAAGACACGGGAAGAGGAAGCGAGAAGAAGATCCGAACAACAATCCGCCATTCAAGAATCGGAAAGGTAATTCGACGAACAATGTCGACCTTAATAAACCAGATCCTCCTCTCCCGATTTCATATCCTTATTCCTCCTTGCAATACCTTCCGTTTTTCAATGCCTATGGCTATGCGTACCCCTGTCTTGTTCCGTACTGGCCCCCCGGTGAGTCTCCCGTGCGTTGCCGGAGTGTTTCTGATGATGGGGTTTCCAATAAGAAGTCTGGGTCCTCTGGATCTTCCCCAGTTTGCAGTTCCTCTGTTGTTTCTGATAATCATCACCATAGTTCTTCATCTCTTGAAGGTACTTTTCAATTTCTTCCAAACCCTAAATAAAATAAAATAAAACAAAACAAAACCGTAATCTGGGTTTTTCTTTTAATGGTTCATCATTATGAGAAATCAGATTTTAAATTAGTTTTGTTGTTGTTCATCTTATTTCAGGTGGTTGTAGCAATTCAAGTAATTCATCAATATCTATGAAGCACGTTAAACCAAAGCCTTGTAATAAGAAATTTGTTGAAGATCAATCTTCTCAAGTGAAGAAATCTTTCTCATCCTCAATGGAGAATGGAAAACCTCCAAAGCCTCAAATCCAAACCCAGAATAATATGGCAAGTTTTGAGCAAATGCCTTGTGTTTCAACAACAGGGATTGGTCCTAATGGTACCAAAATCATAACTACTGGCTTCTTGTATCGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCAGCCGAGTTCGTTAAGCACGGTGGCGGCGGAGAAGTTTCTCACCCTTTGAAACACATTACCGTTCTTCCACCTACTGGAATTTTCCCTTCTTCAATCAGATGAAACTTCATAGATTTTGATCTAGATCTTTGTAGCTTAACATTGTTTGTAGAGATCTGTGGCAATGTGGCTGACGATTTTGATCCGCTAATATTTTGCATTTGTAATCAGTGGTTATTAGAGGTGTGTAATTTTTTTTAATCCGTGTACTTTAACTAATATTTAAAGACAGCCACATTACGACTGCATTCTCTGATGATTTAATACGCAGAAGTGCTTGCAACAAATATTGCAACACCTTGGTTATTAGCTTTTTTACAATGGAGCTGAGGATTTGAACACA

mRNA sequence

ATGAAACATCAACAAAACCTCGATTTACGCCTTTCAATCGGCGGACCTTTCACTAGTTTACCGGAAAAGCTAAATGGGTCTGATCAAGAAAGCGGAGTTACGGATGTCAACAACAGAGTCGACATCCGCCGAAGACACGGGAAGAGGAAGCGAGAAGAAGATCCGAACAACAATCCGCCATTCAAGAATCGGAAAGGTAATTCGACGAACAATGTCGACCTTAATAAACCAGATCCTCCTCTCCCGATTTCATATCCTTATTCCTCCTTGCAATACCTTCCGTTTTTCAATGCCTATGGCTATGCGTACCCCTGTCTTGTTCCGTACTGGCCCCCCGGTGAGTCTCCCGTGCGTTGCCGGAGTGTTTCTGATGATGGGGTTTCCAATAAGAAGTCTGGGTCCTCTGGATCTTCCCCAGTTTGCAGTTCCTCTGTTGTTTCTGATAATCATCACCATAGTTCTTCATCTCTTGAAGGTGGTTGTAGCAATTCAAGTAATTCATCAATATCTATGAAGCACGTTAAACCAAAGCCTTGTAATAAGAAATTTGTTGAAGATCAATCTTCTCAAGTGAAGAAATCTTTCTCATCCTCAATGGAGAATGGAAAACCTCCAAAGCCTCAAATCCAAACCCAGAATAATATGGCAAGTTTTGAGCAAATGCCTTGTGTTTCAACAACAGGGATTGGTCCTAATGGTACCAAAATCATAACTACTGGCTTCTTGTATCGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCAGCCGAGTTCGTTAAGCACGGTGGCGGCGGAGAAGTTTCTCACCCTTTGAAACACATTACCGTTCTTCCACCTACTGGAATTTTCCCTTCTTCAATCAGATGA

Coding sequence (CDS)

ATGAAACATCAACAAAACCTCGATTTACGCCTTTCAATCGGCGGACCTTTCACTAGTTTACCGGAAAAGCTAAATGGGTCTGATCAAGAAAGCGGAGTTACGGATGTCAACAACAGAGTCGACATCCGCCGAAGACACGGGAAGAGGAAGCGAGAAGAAGATCCGAACAACAATCCGCCATTCAAGAATCGGAAAGGTAATTCGACGAACAATGTCGACCTTAATAAACCAGATCCTCCTCTCCCGATTTCATATCCTTATTCCTCCTTGCAATACCTTCCGTTTTTCAATGCCTATGGCTATGCGTACCCCTGTCTTGTTCCGTACTGGCCCCCCGGTGAGTCTCCCGTGCGTTGCCGGAGTGTTTCTGATGATGGGGTTTCCAATAAGAAGTCTGGGTCCTCTGGATCTTCCCCAGTTTGCAGTTCCTCTGTTGTTTCTGATAATCATCACCATAGTTCTTCATCTCTTGAAGGTGGTTGTAGCAATTCAAGTAATTCATCAATATCTATGAAGCACGTTAAACCAAAGCCTTGTAATAAGAAATTTGTTGAAGATCAATCTTCTCAAGTGAAGAAATCTTTCTCATCCTCAATGGAGAATGGAAAACCTCCAAAGCCTCAAATCCAAACCCAGAATAATATGGCAAGTTTTGAGCAAATGCCTTGTGTTTCAACAACAGGGATTGGTCCTAATGGTACCAAAATCATAACTACTGGCTTCTTGTATCGATACTCAAACTTGGAGGTTAGTATTTTGTGTGTCTGCCATGGACAATCCTTTTCGCCAGCCGAGTTCGTTAAGCACGGTGGCGGCGGAGAAGTTTCTCACCCTTTGAAACACATTACCGTTCTTCCACCTACTGGAATTTTCCCTTCTTCAATCAGATGA
Homology
[Close]
BLAST of CSPI03G27710 vs. Swiss-Prot
Match: AFP3_ARATH (Ninja-family protein AFP3 OS=Arabidopsis thaliana GN=AFP3 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.9e-18
Identity = 56/130 (43.08%), Postives = 70/130 (53.85%), Query Frame = 1 

Query: 158 EGGCSNSSNSSISMKHVKPKPCNKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMAS 217
           EG   +S + S  +  V         +    +   KS  +S  + +     +  + +   
Sbjct: 106 EGSIGSSGSGSSGLSEVDT------LLPPVQATTNKSVETSPSSAQSQPENLGKEASQNI 165

Query: 218 FEQMPCVSTTGIGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGEVS 277
            E MP VSTTG GPNG KI   GFLYRY    EV I+CVCHG   SPAEFVKH GGG+V+
Sbjct: 166 IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEEVRIVCVCHGSFLSPAEFVKHAGGGDVA 225

Query: 278 HPLKHITVLP 287
           HPLKHI V P
Sbjct: 226 HPLKHIVVNP 227

[Close]
BLAST of CSPI03G27710 vs. Swiss-Prot
Match: NNJA3_ORYSJ (Ninja-family protein Os03g0419100 OS=Oryza sativa subsp. japonica GN=Os03g0419100 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 44/72 (61.11%), Postives = 52/72 (72.22%), Query Frame = 1 

Query: 221 MPCVSTTGIGPNGTKIITTGFLYRYSNLE-VSILCVCHGQSFSPAEFVKHGGGGEVSHPL 280
           MP VS+   GPNG KI   GFLY+Y   E VSI+CVCHG+  SPAEFVKH GGG+VS+PL
Sbjct: 237 MPMVSSKVEGPNGRKI--DGFLYKYRKGEDVSIMCVCHGKFHSPAEFVKHAGGGDVSNPL 296

Query: 281 KHITVLPPTGIF 292
           +HI V P   +F
Sbjct: 297 RHIVVNPSPSVF 306

[Close]
BLAST of CSPI03G27710 vs. Swiss-Prot
Match: NNJA2_ORYSJ (Ninja-family protein Os07g0602900 OS=Oryza sativa subsp. japonica GN=Os07g0602900 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 4.8e-16
Identity = 42/74 (56.76%), Postives = 51/74 (68.92%), Query Frame = 1 

Query: 219 EQMPCVSTTGIGPNGTKIITTGFLYRYSNLE-VSILCVCHGQSFSPAEFVKHGGGGEVSH 278
           E MP VS+   GPNG KI   GFLY+Y   E V I+CVCHG   +PAEFVKH GGG+V++
Sbjct: 247 EDMPMVSSRVDGPNGRKI--DGFLYKYRKREEVRIVCVCHGNFLTPAEFVKHAGGGDVTN 306

Query: 279 PLKHITVLPPTGIF 292
           PL+HI V P   +F
Sbjct: 307 PLRHIVVNPSRSVF 318

[Close]
BLAST of CSPI03G27710 vs. Swiss-Prot
Match: NNJA5_MAIZE (Ninja-family protein 5 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.4e-15
Identity = 42/77 (54.55%), Postives = 49/77 (63.64%), Query Frame = 1 

Query: 216 ASFEQMPCVSTTGIGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGE 275
           A  E MP VS    GP G K  T GFLY+Y    EV I+CVCHG   +PAEFV+H GGGE
Sbjct: 232 AMAEDMPMVSYKAEGPGGRK--TDGFLYKYRKGEEVRIVCVCHGSFLTPAEFVEHAGGGE 291

Query: 276 VSHPLKHITVLPPTGIF 292
           V +PL+HI V P   +F
Sbjct: 292 VPNPLRHIVVNPQQSVF 306

[Close]
BLAST of CSPI03G27710 vs. Swiss-Prot
Match: NNJA3_WHEAT (Ninja-family protein 3 OS=Triticum aestivum GN=AFP-D1 PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.4e-15
Identity = 41/74 (55.41%), Postives = 50/74 (67.57%), Query Frame = 1 

Query: 219 EQMPCVSTTGIGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGEVSH 278
           E MP VS+   GPN  KI   GFLY+Y    EV I+CVCHG   +PAEFVKH GGG+V++
Sbjct: 238 EDMPIVSSKVDGPNSKKI--DGFLYKYRKGEEVRIVCVCHGNFLTPAEFVKHAGGGDVTN 297

Query: 279 PLKHITVLPPTGIF 292
           PL+HI V P   +F
Sbjct: 298 PLRHIVVNPSPSVF 309

[Close]
BLAST of CSPI03G27710 vs. TrEMBL
Match: A0A0A0L8T7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G633290 PE=4 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 3.0e-166
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 1 

Query: 1   MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP 60
           MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP
Sbjct: 1   MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP 60

Query: 61  FKNRKGNSTNNVDLNKPDPPLPISYPYSSLQYLPFFNAYGYAYPCLVPYWPPGESPVRCR 120
           FKNRKGN TNNVDLNKPDPPLPISYPYSS QYLPFFNAYGYAYPCLVPYWPPGESPVRCR
Sbjct: 61  FKNRKGNLTNNVDLNKPDPPLPISYPYSSFQYLPFFNAYGYAYPCLVPYWPPGESPVRCR 120

Query: 121 SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN 180
           SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN
Sbjct: 121 SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN 180

Query: 181 KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG 240
           KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG
Sbjct: 181 KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG 240

Query: 241 FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR 297
           FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR
Sbjct: 241 FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR 296

[Close]
BLAST of CSPI03G27710 vs. TrEMBL
Match: F6HCQ5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0216g00060 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.2e-26
Identity = 120/306 (39.22%), Postives = 152/306 (49.67%), Query Frame = 1 

Query: 22  EKLNGSDQESGVTDVNNRVDIRRRHGKRK-REEDPNNNPPFKNRKG--NSTNNVDLNKP- 81
           EK  G     GV D   R++ +R   + K RE   NN     + K   N  N+  +  P 
Sbjct: 57  EKKKGMRNGGGVGDERMRLEAQRLQNRVKDRERRENNEANLTHHKSYINHRNHGIVEGPI 116

Query: 82  DPPLPISYPY-SSLQYLPFFNAYGYAYPCLVPYWPPG-------ESPVR--C--RSVSDD 141
              +PI Y + S +QY+P       A+PC+VP   P          PVR  C  RSV  D
Sbjct: 117 QMMVPIKYAHPSKVQYVPL------AFPCVVPCGAPATWLQPVKSLPVRQGCGGRSVLSD 176

Query: 142 G-------VSNKKSGSSGSSPVCS--------SSVVSDNHHHSSSSLEGGCSNSSNS--S 201
           G       VS++ SGSS    + S        SS       HS SS +   S  + S  S
Sbjct: 177 GWTNSSASVSHQDSGSSDIGSLSSHTRTAPPQSSEAQGQSEHSVSSYQKDSSQGTRSDKS 236

Query: 202 ISMKHVKPKPC-------NKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMA-SFEQ 261
            S       P        N    E   S +KK+   + + G+PPKP+ Q  +  A S EQ
Sbjct: 237 TSFGEKVSTPVKDPTTRRNSSTGESPPSPLKKARDGNGDLGEPPKPRNQNHHPSAPSLEQ 296

Query: 262 MPCVSTTGIGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLK 287
           MPCVSTTG GPNG  I  TGFLYRY+  E+SI+CVCHG SFSPA FV+H GG +VSHPL+
Sbjct: 297 MPCVSTTGNGPNGKTI--TGFLYRYTKAEISIVCVCHGSSFSPAGFVEHAGGIDVSHPLR 354

[Close]
BLAST of CSPI03G27710 vs. TrEMBL
Match: A0A067K266_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17508 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.6e-26
Identity = 107/284 (37.68%), Postives = 136/284 (47.89%), Query Frame = 1 

Query: 81  LPISYPYSSLQYLPFFNAYGYAYPC----LVPYW-PPGE--SPVRCRSV----------- 140
           LP+ Y +  LQY P  N  G+ YPC    ++P W   GE  + V+ +SV           
Sbjct: 187 LPVQYAHPPLQYGPPTN--GFVYPCANVNVMPCWFTAGEERNVVQVQSVVSYGFVPFQAA 246

Query: 141 SDDGVS----------------NKKSGSSGSSPVCSSSVVSDNH---------------- 200
           SD G +                NKK GS+GS P+C+SS  SD+                 
Sbjct: 247 SDPGHNLGSGYDSEQNGSRDNGNKKDGSNGS-PMCASSTSSDHRSFSNEGGGSSDTRSRS 306

Query: 201 ----------------------HHSSSSLEGGCSNSSNSSISMKHVKPKPCNKKFVEDQS 260
                                  HS++S     +   N  I     +PK   K  +E ++
Sbjct: 307 SPSVPEQPQLNDSKAKEAKGQSEHSATSELIEAAQVENKMIQSSSTEPKGEAK--IEKET 366

Query: 261 SQVKKSF------SSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTGFL 287
           S   + F      S S + GKPPKPQ    +N +S   MPCVSTTG GPNG  I   GFL
Sbjct: 367 SPTNQRFATPLMKSKSTDLGKPPKPQKLNHSN-SSLPSMPCVSTTGNGPNGRTI--NGFL 426

[Close]
BLAST of CSPI03G27710 vs. TrEMBL
Match: A5AEH4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_044375 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.4e-25
Identity = 116/306 (37.91%), Postives = 153/306 (50.00%), Query Frame = 1 

Query: 22  EKLNGSDQESGVTDVNNRVDIRRRHGKRK-REEDPNNNPPFKNRKG--NSTNNVDLNKP- 81
           EK  G     GV D   R++ +R   + K RE   NN     + K   N  N+  +  P 
Sbjct: 673 EKKKGMRNGGGVGDERMRLEAQRLQNRVKDRERRENNEANLTHHKSYINHRNHGIVEGPI 732

Query: 82  DPPLPISYPY-SSLQYLPFFNAYGYAYPCLVPYWPPGE--SPVRC---------RSVSDD 141
              +PI Y + S +QY+P       A+PC+VP   P     PV+          RSV  D
Sbjct: 733 QMMVPIKYAHPSKVQYVPL------AFPCVVPCGAPATWLQPVKSLPVRQGYGGRSVLSD 792

Query: 142 G-------VSNKKSGSSGSSPVCS--------SSVVSDNHHHSSSSLEG----GCSNSSN 201
           G       VS++ SGSS    + S        SS       HS SS +     G S+  +
Sbjct: 793 GWTNSSASVSHQDSGSSDIGSLSSHTRTAPPQSSEAQGQSEHSVSSYQKDSSQGTSSDKS 852

Query: 202 SSISMKHVKP--KPCNKKFV---EDQSSQVKKSFSSSMENGKPPKPQIQTQNNMA-SFEQ 261
           +S   K   P   P  ++     E   S +KK+   + +  +PP P+ Q  +  A S EQ
Sbjct: 853 TSFGEKVSTPVKDPTTRRXSSTGESPPSPLKKARDGNGDLXEPPXPRNQNHHPSAPSLEQ 912

Query: 262 MPCVSTTGIGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLK 287
           MPCVSTTG GPNG  I  TGFLYRY+  E+SI+CVCHG SFSPA FV+H GG +VSHPL+
Sbjct: 913 MPCVSTTGNGPNGKTI--TGFLYRYTKAEISIVCVCHGSSFSPAGFVEHAGGXDVSHPLR 970

[Close]
BLAST of CSPI03G27710 vs. TrEMBL
Match: A0A067FIK5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011821mg PE=4 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.7e-25
Identity = 62/93 (66.67%), Postives = 68/93 (73.12%), Query Frame = 1 

Query: 194 SFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTGFLYRYSNLEVSIL 253
           S  S  + GKPPKPQ Q+QN   S   MPCVSTTG GPNG  I   GFLYRY+  EVSI+
Sbjct: 385 SKESKKDVGKPPKPQTQSQNT-PSLPHMPCVSTTGNGPNGKTI--NGFLYRYTKTEVSIV 444

Query: 254 CVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLP 287
           CVCHG SFSPAEFV H GG +VS PLKHITV+P
Sbjct: 445 CVCHGTSFSPAEFVLHAGGTDVSQPLKHITVIP 474

[Close]
BLAST of CSPI03G27710 vs. TAIR10
Match: AT3G07250.1 (AT3G07250.1 nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein)

HSP 1 Score: 119.4 bits (298), Expect = 3.8e-27
Identity = 75/161 (46.58%), Postives = 93/161 (57.76%), Query Frame = 1 

Query: 138 SPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCNKKFV------------E 197
           SP+C+SS VSD    SSS  EGG  +    S   K V+  P N                +
Sbjct: 126 SPICTSSSVSDPS--SSSRQEGGSGDIGAQSGQTKQVR-SPVNNILTGTEQTVHNTDGSK 185

Query: 198 DQSSQVKKSFSSSMENGKPPKPQIQTQNN--MASFEQMPCVSTTGIGPNGTKIITTGFLY 257
           D  +Q   + S + E+GKPPKP   +  N  +  F QMPCV++TG GP G  +   GFLY
Sbjct: 186 DAETQEWSNSSVTKESGKPPKPHTNSNGNGSLLPFAQMPCVTSTGNGPEGKTV--NGFLY 245

Query: 258 RYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITV 285
           RYS  E+SI+CVCHG SFSPAEF+ H GG  VSHPL+HITV
Sbjct: 246 RYSKSEISIICVCHGTSFSPAEFIVHAGGTNVSHPLRHITV 281

[Close]
BLAST of CSPI03G27710 vs. TAIR10
Match: AT3G29575.1 (AT3G29575.1 ABI five binding protein 3)

HSP 1 Score: 93.6 bits (231), Expect = 2.2e-19
Identity = 56/130 (43.08%), Postives = 70/130 (53.85%), Query Frame = 1 

Query: 158 EGGCSNSSNSSISMKHVKPKPCNKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMAS 217
           EG   +S + S  +  V         +    +   KS  +S  + +     +  + +   
Sbjct: 106 EGSIGSSGSGSSGLSEVDT------LLPPVQATTNKSVETSPSSAQSQPENLGKEASQNI 165

Query: 218 FEQMPCVSTTGIGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGEVS 277
            E MP VSTTG GPNG KI   GFLYRY    EV I+CVCHG   SPAEFVKH GGG+V+
Sbjct: 166 IEDMPFVSTTGDGPNGKKI--NGFLYRYRKGEEVRIVCVCHGSFLSPAEFVKHAGGGDVA 225

Query: 278 HPLKHITVLP 287
           HPLKHI V P
Sbjct: 226 HPLKHIVVNP 227

[Close]
BLAST of CSPI03G27710 vs. TAIR10
Match: AT1G13740.1 (AT1G13740.1 ABI five binding protein 2)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-14
Identity = 59/182 (32.42%), Postives = 86/182 (47.25%), Query Frame = 1 

Query: 126 GVSNKKSGSSGSSPVCSSSVVSDNHHHSSSS---------LEGGCSNSSNS------SIS 185
           GV +   G++G     S S + + +   SS+         +  GCS++S S      S++
Sbjct: 184 GVDSDGGGATGGGSSSSLSELDNKNQQGSSNSCNDERSPKIVAGCSSNSGSQGTERPSVT 243

Query: 186 MKHVKPKPCNKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIG 245
             +   +  N+K V  + S  +K    +   G                  MPCV T G G
Sbjct: 244 RANKVNENENEKRVRSEDSVDRKGKGMATSTG---------------LVDMPCVFTKGDG 303

Query: 246 PNGTKIITTGFLYRYSN-LEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTG 292
           PNG ++   G LY+Y    EV I+C+CHG   +PAEFVKHGGGG+V  PL+HI V   + 
Sbjct: 304 PNGRRV--DGILYKYGKGEEVRIMCICHGSFLTPAEFVKHGGGGDVDRPLRHIVVNTSSS 348

[Close]
BLAST of CSPI03G27710 vs. TAIR10
Match: AT1G69260.1 (AT1G69260.1 ABI five binding protein)

HSP 1 Score: 74.7 bits (182), Expect = 1.1e-13
Identity = 60/177 (33.90%), Postives = 88/177 (49.72%), Query Frame = 1 

Query: 118 RCRSVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPK 177
           R R     G+    + ++ S  +   S   D+   S  SL GG +  S+SS+S    K  
Sbjct: 168 RRRGRPSSGLPRWSATANKSGLLRQHSAGLDSLQVSGESLGGGRAAGSSSSVSELETKAS 227

Query: 178 PCNKKFVEDQSSQVKKSFS---------SSMENGKPPKPQIQTQNNMASFEQMPCVSTTG 237
               + +   +   +++ +         SS++     +PQ + ++      +MPCV T G
Sbjct: 228 SDEARSLPSTTQPQQETTTKPTNRLRRLSSVDMNMKMEPQGKGKS------EMPCVFTKG 287

Query: 238 IGPNGTKIITTGFLYRY-SNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITV 285
            GPNG ++   G LYRY S  EV I+CVCHG   SPA+FVKH GG  V HPL+HI V
Sbjct: 288 DGPNGKRV--DGILYRYGSGEEVRIMCVCHGDFLSPADFVKHAGGPHVDHPLRHIVV 336

[Close]
BLAST of CSPI03G27710 vs. TAIR10
Match: AT3G02140.1 (AT3G02140.1 AFP2 (ABI five-binding protein 2) family protein)

HSP 1 Score: 72.4 bits (176), Expect = 5.3e-13
Identity = 45/105 (42.86%), Postives = 57/105 (54.29%), Query Frame = 1 

Query: 200 ENGKPPKPQIQTQNNMAS------FEQMPCVSTTGIGPNGTKIITTGFLYRY--SNLEVS 259
           +NG   K Q     N          E MPCVST  +G +G ++   GFLY Y  +  EV 
Sbjct: 214 KNGNTAKKQKNNVENSGMEKARNILEDMPCVSTRDVGADGKRV--EGFLYWYGGNKEEVK 273

Query: 260 ILCVCHGQSFSPAEFVKHGG-------GGEVS-HPLKHITVLPPT 289
           I+CVCHG   SPAEFV+HGG       GG+V  +PL+HI V  P+
Sbjct: 274 IVCVCHGSFLSPAEFVRHGGGTVSDDDGGDVMINPLRHIVVKLPS 316

[Close]
BLAST of CSPI03G27710 vs. NCBI nr
Match: gi|778682200|ref|XP_004144458.2| (PREDICTED: ninja-family protein AFP3-like [Cucumis sativus])

HSP 1 Score: 592.4 bits (1526), Expect = 4.3e-166
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 1 

Query: 1   MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP 60
           MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP
Sbjct: 1   MKHQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPP 60

Query: 61  FKNRKGNSTNNVDLNKPDPPLPISYPYSSLQYLPFFNAYGYAYPCLVPYWPPGESPVRCR 120
           FKNRKGN TNNVDLNKPDPPLPISYPYSS QYLPFFNAYGYAYPCLVPYWPPGESPVRCR
Sbjct: 61  FKNRKGNLTNNVDLNKPDPPLPISYPYSSFQYLPFFNAYGYAYPCLVPYWPPGESPVRCR 120

Query: 121 SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN 180
           SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN
Sbjct: 121 SVSDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCN 180

Query: 181 KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG 240
           KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG
Sbjct: 181 KKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMASFEQMPCVSTTGIGPNGTKIITTG 240

Query: 241 FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR 297
           FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR
Sbjct: 241 FLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHITVLPPTGIFPSSIR 296

[Close]
BLAST of CSPI03G27710 vs. NCBI nr
Match: gi|659120872|ref|XP_008460392.1| (PREDICTED: uncharacterized protein LOC103499221 [Cucumis melo])

HSP 1 Score: 356.7 bits (914), Expect = 4.0e-95
Identity = 183/208 (87.98%), Postives = 188/208 (90.38%), Query Frame = 1 

Query: 3   HQQNLDLRLSIGGPFTSLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPPFK 62
           HQQNLDLRLSIGGPF +LPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPPFK
Sbjct: 7   HQQNLDLRLSIGGPFNNLPEKLNGSDQESGVTDVNNRVDIRRRHGKRKREEDPNNNPPFK 66

Query: 63  NRKGNSTNNVDLNKPDPPLPISYPYSSLQYLPFFNAYGYAYPCLVPYWPPGESPVRCRSV 122
           NR+GN TNNVDLNKPDPPL ISYPYSSLQY+PFFN YGYAYPCLVPYWPP ESPV CR V
Sbjct: 67  NRRGNFTNNVDLNKPDPPLQISYPYSSLQYVPFFNGYGYAYPCLVPYWPPDESPVGCRIV 126

Query: 123 SDDGVSNKKSGSSGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCNKK 182
           SDDGVSNKKSGS GSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCNKK
Sbjct: 127 SDDGVSNKKSGSCGSSPVCSSSVVSDNHHHSSSSLEGGCSNSSNSSISMKHVKPKPCNKK 186

Query: 183 FVEDQSSQVKK---SFSSSMENGKPPKP 208
           FVED+SSQVKK    F+   E  K   P
Sbjct: 187 FVEDESSQVKKIFLIFNGEWETSKASNP 214

[Close]
BLAST of CSPI03G27710 vs. NCBI nr
Match: gi|694393592|ref|XP_009372227.1| (PREDICTED: ninja-family protein 2-like [Pyrus x bretschneideri])

HSP 1 Score: 136.7 bits (343), Expect = 6.5e-29
Identity = 116/354 (32.77%), Postives = 164/354 (46.33%), Query Frame = 1 

Query: 16  PFTSLPEKLNGSDQESGVTDVNNRVD---IRRRHGKRKREE-------DPN--NNPPFKN 75
           P    P  L  S+ ES + D + + +   +RR+  KRKRE+       DP    +P  K 
Sbjct: 54  PNAPSPSPLESSEPESALLDPHTKREMQALRRQEAKRKREQKKGRGQNDPQVEEHPAVKK 113

Query: 76  RKGNSTNNVDLN----------KPDPPLPISYPYSSLQYLPFFNAYGYAYPCLVPYWPP- 135
            +  +  NV++N          +     P  YPY  +Q++P+ N  G++YPC++P W P 
Sbjct: 114 ERTETNVNVNVNLNLSNGQTELRCPVQQPGQYPYPPVQFVPYMN--GFSYPCVMPCWAPS 173

Query: 136 --------------GESPVRCRSVSDDGVSNK--------KSGSSGSSPVCSSSVVSDNH 195
                         G+    C +  + G            K  +S  SP+CSSS VSD+ 
Sbjct: 174 GGGGFRPFQAHKDTGKKKNGCETEQNSGSGKTAPVNGGLGKKPASNVSPMCSSSTVSDHQ 233

Query: 196 HHSSSS--LEGGCSNS--------------------SNSSISMKHVKPKPCNKKFVEDQS 255
             +S    L GG S S                    SNS  + + + PK   K    + S
Sbjct: 234 SAASQDGMLGGGSSESKSHSSQSIQENTEPVSNSLESNSEGNEQKLNPKTEPKSETAEAS 293

Query: 256 SQVK---------------KSFSSSMENGKPPKPQIQTQ-NNMASFEQMPCVSTTGIGPN 287
              K               K  + +    KPPKPQI    ++  S  QMP VSTTG GPN
Sbjct: 294 PTPKPISPKQEYSPPIPPLKDVTKTDPIAKPPKPQISKNGSSTVSLPQMPYVSTTGTGPN 353

[Close]
BLAST of CSPI03G27710 vs. NCBI nr
Match: gi|470107690|ref|XP_004290175.1| (PREDICTED: ninja-family protein AFP2-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 135.6 bits (340), Expect = 1.4e-28
Identity = 111/312 (35.58%), Postives = 156/312 (50.00%), Query Frame = 1 

Query: 27  SDQESGVTDVNNRVDI---RRRHGKRKREEDPNN-------NPPFKNR----KGNSTNNV 86
           S+ ES V D   R +I   RR+  KRKREE            PP K      + ++  NV
Sbjct: 64  SEAESDVLDPLARREIQALRRQEAKRKREEKKTRALIGEQQQPPAKKNGVLSQDDNAGNV 123

Query: 87  DLNKPDPPLPISYPYSSLQYLPFFNAYGYAYPCLVPYWPP----GESPVRCRSVSDDGVS 146
           + +   P  P+ YPY+ +Q++P+ N  G+AYPC+VP W P    G    + + + ++G +
Sbjct: 124 E-HHHYPVGPVQYPYAPVQFIPYSN--GFAYPCVVPCWAPNGGGGGGFRQFQDLGNNGCN 183

Query: 147 NKKSGSSGS----SPVCSSS-------------VVSDNHHHSS--SSLEGGCSNSSNSSI 206
            +++G + S    SP+CSSS               SD   HSS  S   G    +++ SI
Sbjct: 184 PEQNGKTASLNNGSPICSSSTVSEHYSAASHEGGSSDAKSHSSRLSGSIGSDHTATSHSI 243

Query: 207 SMKH---------------VKPKPCNKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNN 266
              H                +P P  K    ++         + ME  KP    I+ +  
Sbjct: 244 ESNHYLVIRPTKEEPAPEITEPVPIAKPISSNEECPPPPLKETKMEPPKPKTENIKNREK 303

Query: 267 MASFEQMPCVSTTGIGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGE 287
             S  QMP VSTTG GPNG  +   GFLYRYS  ++SI+CVCHG +FSPAEFV+H GG +
Sbjct: 304 TPSMPQMPYVSTTGNGPNGKTV--HGFLYRYSKSDISIVCVCHGSTFSPAEFVQHAGGAD 363

[Close]
BLAST of CSPI03G27710 vs. NCBI nr
Match: gi|296084620|emb|CBI25670.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 128.6 bits (322), Expect = 1.8e-26
Identity = 103/243 (42.39%), Postives = 128/243 (52.67%), Query Frame = 1 

Query: 81  LPISYPY-SSLQYLPFFNAYGYAYPCLVPYWPPG-------ESPVR--C--RSVSDDG-- 140
           +PI Y + S +QY+P       A+PC+VP   P          PVR  C  RSV  DG  
Sbjct: 2   VPIKYAHPSKVQYVPL------AFPCVVPCGAPATWLQPVKSLPVRQGCGGRSVLSDGWT 61

Query: 141 -----VSNKKSGSSGSSPVCS--------SSVVSDNHHHSSSSLEGGCSNSSNS--SISM 200
                VS++ SGSS    + S        SS       HS SS +   S  + S  S S 
Sbjct: 62  NSSASVSHQDSGSSDIGSLSSHTRTAPPQSSEAQGQSEHSVSSYQKDSSQGTRSDKSTSF 121

Query: 201 KHVKPKPC-------NKKFVEDQSSQVKKSFSSSMENGKPPKPQIQTQNNMA-SFEQMPC 260
                 P        N    E   S +KK+   + + G+PPKP+ Q  +  A S EQMPC
Sbjct: 122 GEKVSTPVKDPTTRRNSSTGESPPSPLKKARDGNGDLGEPPKPRNQNHHPSAPSLEQMPC 181

Query: 261 VSTTGIGPNGTKIITTGFLYRYSNLEVSILCVCHGQSFSPAEFVKHGGGGEVSHPLKHIT 287
           VSTTG GPNG  I  TGFLYRY+  E+SI+CVCHG SFSPA FV+H GG +VSHPL+HIT
Sbjct: 182 VSTTGNGPNGKTI--TGFLYRYTKAEISIVCVCHGSSFSPAGFVEHAGGIDVSHPLRHIT 236

The following BLAST results are available for this feature:
BLAST of CSPI03G27710 vs. Swiss-Prot
Match NameE-valueIdentityDescription
AFP3_ARATH3.9e-1843.08Ninja-family protein AFP3 OS=Arabidopsis thaliana GN=AFP3 PE=1 SV=1[more]
NNJA3_ORYSJ1.3e-1661.11Ninja-family protein Os03g0419100 OS=Oryza sativa subsp. japonica GN=Os03g041910... [more]
NNJA2_ORYSJ4.8e-1656.76Ninja-family protein Os07g0602900 OS=Oryza sativa subsp. japonica GN=Os07g060290... [more]
NNJA5_MAIZE2.4e-1554.55Ninja-family protein 5 OS=Zea mays PE=2 SV=1[more]
NNJA3_WHEAT2.4e-1555.41Ninja-family protein 3 OS=Triticum aestivum GN=AFP-D1 PE=3 SV=1[more]
BLAST of CSPI03G27710 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8T7_CUCSA3.0e-16699.32Uncharacterized protein OS=Cucumis sativus GN=Csa_3G633290 PE=4 SV=1[more]
F6HCQ5_VITVI1.2e-2639.22Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0216g00060 PE=4 SV=... [more]
A0A067K266_JATCU3.6e-2637.68Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17508 PE=4 SV=1[more]
A5AEH4_VITVI1.4e-2537.91Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_044375 PE=4 SV=1[more]
A0A067FIK5_CITSI6.7e-2566.67Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011821mg PE=4 SV=1[more]
BLAST of CSPI03G27710 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G07250.13.8e-2746.58 nuclear transport factor 2 (NTF2) family protein / RNA recognition m... [more]
AT3G29575.12.2e-1943.08 ABI five binding protein 3[more]
AT1G13740.11.3e-1432.42 ABI five binding protein 2[more]
AT1G69260.11.1e-1333.90 ABI five binding protein[more]
AT3G02140.15.3e-1342.86 AFP2 (ABI five-binding protein 2) family protein[more]
BLAST of CSPI03G27710 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778682200|ref|XP_004144458.2|4.3e-16699.32PREDICTED: ninja-family protein AFP3-like [Cucumis sativus][more]
gi|659120872|ref|XP_008460392.1|4.0e-9587.98PREDICTED: uncharacterized protein LOC103499221 [Cucumis melo][more]
gi|694393592|ref|XP_009372227.1|6.5e-2932.77PREDICTED: ninja-family protein 2-like [Pyrus x bretschneideri][more]
gi|470107690|ref|XP_004290175.1|1.4e-2835.58PREDICTED: ninja-family protein AFP2-like [Fragaria vesca subsp. vesca][more]
gi|296084620|emb|CBI25670.3|1.8e-2642.39unnamed protein product [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
Vocabulary: Biological Process
TermDefinition
GO:0007165signal transduction
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007165 signal transduction
biological_process GO:0006621 protein retention in ER lumen
cellular_component GO:0005575 cellular_component
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function
molecular_function GO:0046923 ER retention sequence binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G27710.1CSPI03G27710.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR31413:SF4NINJA-FAMILY PROTEIN AFP1-RELATEDcoord: 203..286
score: 3.6

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI03G27710Cla002701Watermelon (97103) v1cpiwmB225
CSPI03G27710Csa3G633290Cucumber (Chinese Long) v2cpicuB120
CSPI03G27710MELO3C022721Melon (DHL92) v3.5.1cpimeB200
CSPI03G27710ClCG07G002130Watermelon (Charleston Gray)cpiwcgB258
CSPI03G27710Lsi05G011360Bottle gourd (USVL1VR-Ls)cpilsiB199
CSPI03G27710MELO3C022721.2Melon (DHL92) v3.6.1cpimedB194
CSPI03G27710CsaV3_3G030080Cucumber (Chinese Long) v3cpicucB144
CSPI03G27710Cla97C07G130430Watermelon (97103) v2cpiwmbB244
CSPI03G27710Bhi05G000040Wax gourdcpiwgoB309
CSPI03G27710Cucsa.150070Cucumber (Gy14) v1cgycpiB221
CSPI03G27710CmaCh19G001730Cucurbita maxima (Rimu)cmacpiB516
CSPI03G27710CmaCh02G016680Cucurbita maxima (Rimu)cmacpiB606
CSPI03G27710CmoCh19G001910Cucurbita moschata (Rifu)cmocpiB506
CSPI03G27710CmoCh02G017110Cucurbita moschata (Rifu)cmocpiB595
CSPI03G27710Cp4.1LG15g01500Cucurbita pepo (Zucchini)cpecpiB242
CSPI03G27710CsGy3G025720Cucumber (Gy14) v2cgybcpiB110
CSPI03G27710Carg08890Silver-seed gourdcarcpiB0816
CSPI03G27710Carg26381Silver-seed gourdcarcpiB0325
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI03G27710Cucurbita pepo (Zucchini)cpecpiB718
CSPI03G27710Bottle gourd (USVL1VR-Ls)cpilsiB175
CSPI03G27710Cucumber (Gy14) v2cgybcpiB316
CSPI03G27710Cucumber (Gy14) v1cgycpiB078
CSPI03G27710Cucumber (Chinese Long) v2cpicuB166
CSPI03G27710Watermelon (Charleston Gray)cpiwcgB189