CSPI03G07280 (gene) Wild cucumber (PI 183967)

NameCSPI03G07280
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionProtein transport protein Sec61 subunit beta
LocationChr3 : 6461651 .. 6461899 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTAGGTGGAGGAGGTCCCCAGAGAGGAAGTGCGGCAGCTACTGCCAGCATGCGCAGGAGAAGAACAACAAGTTCTGGTACATCTGGAGGGGCAGCTGGAACGATGCTGCAGTTTTACACGGATGATGCTCCTGGACTCAAGATATCCCCGAATGTTGTTCTCGTCATGAGCATTGGTTTCATTGCTTTTGTTGCCGTTCTCCATGTTATGGGTAAGCTGTACTTCGTGCGAAGAGAAGCCTAG

mRNA sequence

ATGGCTTTAGGTGGAGGAGGTCCCCAGAGAGGAAGTGCGGCAGCTACTGCCAGCATGCGCAGGAGAAGAACAACAAGTTCTGGTACATCTGGAGGGGCAGCTGGAACGATGCTGCAGTTTTACACGGATGATGCTCCTGGACTCAAGATATCCCCGAATGTTGTTCTCGTCATGAGCATTGGTTTCATTGCTTTTGTTGCCGTTCTCCATGTTATGGGTAAGCTGTACTTCGTGCGAAGAGAAGCCTAG

Coding sequence (CDS)

ATGGCTTTAGGTGGAGGAGGTCCCCAGAGAGGAAGTGCGGCAGCTACTGCCAGCATGCGCAGGAGAAGAACAACAAGTTCTGGTACATCTGGAGGGGCAGCTGGAACGATGCTGCAGTTTTACACGGATGATGCTCCTGGACTCAAGATATCCCCGAATGTTGTTCTCGTCATGAGCATTGGTTTCATTGCTTTTGTTGCCGTTCTCCATGTTATGGGTAAGCTGTACTTCGTGCGAAGAGAAGCCTAG
BLAST of CSPI03G07280 vs. Swiss-Prot
Match: SC61B_ARATH (Protein transport protein Sec61 subunit beta OS=Arabidopsis thaliana GN=At2g45070 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.9e-27
Identity = 67/81 (82.72%), Postives = 72/81 (88.89%), Query Frame = 1

Query: 3  LGGGGPQRGSAAATASMRRRRTTSS----GTSGGAAGTMLQFYTDDAPGLKISPNVVLVM 62
          +G G PQRGSAAATASMRRR+ TS     G SGGAAG+MLQFYTDDAPGLKISPNVVL+M
Sbjct: 2  VGSGAPQRGSAAATASMRRRKPTSGAGGGGASGGAAGSMLQFYTDDAPGLKISPNVVLIM 61

Query: 63 SIGFIAFVAVLHVMGKLYFVR 80
          SIGFIAFVAVLHVMGKLYFV+
Sbjct: 62 SIGFIAFVAVLHVMGKLYFVK 82

BLAST of CSPI03G07280 vs. Swiss-Prot
Match: SC61B_YARLI (Protein transport protein Sec61 subunit beta OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=SBH1 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.3e-07
Identity = 30/61 (49.18%), Postives = 43/61 (70.49%), Query Frame = 1

Query: 15 ATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVMGK 74
          A AS R   T S G +GG++ TML+ YTD++ GLK+ P VV+V+S+GFI  V  LH++ K
Sbjct: 26 AKASQRPTSTRSVG-AGGSSSTMLKLYTDESQGLKVDPVVVMVLSLGFIFSVVALHILAK 85

Query: 75 L 76
          +
Sbjct: 86 V 85

BLAST of CSPI03G07280 vs. Swiss-Prot
Match: SC61B_DICDI (Protein transport protein Sec61 subunit beta OS=Dictyostelium discoideum GN=sec61b PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 26/53 (49.06%), Postives = 38/53 (71.70%), Query Frame = 1

Query: 22 RRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSIGFIAFVAVLHVMGK 75
          +R  ++   GG   +M++FY++DA GLK+ P  VL MS+ FIAFV +LH+MGK
Sbjct: 7  QRAPATVNKGG--NSMMKFYSEDAIGLKVGPTAVLFMSLIFIAFVIILHIMGK 57

BLAST of CSPI03G07280 vs. Swiss-Prot
Match: SC61B_CANLF (Protein transport protein Sec61 subunit beta OS=Canis lupus familiaris GN=SEC61B PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 6.2e-06
Identity = 34/78 (43.59%), Postives = 47/78 (60.26%), Query Frame = 1

Query: 5  GGGPQRGSAAATA--SMRRRRTTSSGT------SGGAAGTMLQFYTDDAPGLKISPNVVL 64
          G  P +  AA  A  ++R+R+  S GT      +    G M +FYT+D+PGLK+ P  VL
Sbjct: 15 GRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVL 74

Query: 65 VMSIGFIAFVAVLHVMGK 75
          VMS+ FIA V +LH+ GK
Sbjct: 75 VMSLLFIASVFMLHIWGK 92

BLAST of CSPI03G07280 vs. Swiss-Prot
Match: SC61B_HUMAN (Protein transport protein Sec61 subunit beta OS=Homo sapiens GN=SEC61B PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 6.2e-06
Identity = 34/78 (43.59%), Postives = 47/78 (60.26%), Query Frame = 1

Query: 5  GGGPQRGSAAATA--SMRRRRTTSSGT------SGGAAGTMLQFYTDDAPGLKISPNVVL 64
          G  P +  AA  A  ++R+R+  S GT      +    G M +FYT+D+PGLK+ P  VL
Sbjct: 15 GRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVL 74

Query: 65 VMSIGFIAFVAVLHVMGK 75
          VMS+ FIA V +LH+ GK
Sbjct: 75 VMSLLFIASVFMLHIWGK 92

BLAST of CSPI03G07280 vs. TrEMBL
Match: A0A0A0L6Y8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G117430 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 1.7e-34
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. TrEMBL
Match: I3T8K1_LOTJA (Uncharacterized protein OS=Lotus japonicus PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 1.8e-31
Identity = 77/82 (93.90%), Postives = 77/82 (93.90%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGG  PQRGSAAA ASMRRRRTTS G SGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGAAPQRGSAAAAASMRRRRTTSGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. TrEMBL
Match: W9QQ35_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_021777 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 8.7e-31
Identity = 74/82 (90.24%), Postives = 77/82 (93.90%), Query Frame = 1

Query: 1   MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
           M LGG  P RGSAAATASMRRRRTTSSG SGGAAGTMLQFYTDDAPGLKISPNVVL+MSI
Sbjct: 511 MVLGGAAPPRGSAAATASMRRRRTTSSGASGGAAGTMLQFYTDDAPGLKISPNVVLIMSI 570

Query: 61  GFIAFVAVLHVMGKLYFVRREA 83
           GFIAFVA+LHVMGKLYFVRRE+
Sbjct: 571 GFIAFVAILHVMGKLYFVRRES 592

BLAST of CSPI03G07280 vs. TrEMBL
Match: A0A151R1A8_CAJCA (Protein transport protein Sec61 subunit beta OS=Cajanus cajan GN=KK1_042494 PE=4 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.9e-30
Identity = 76/83 (91.57%), Postives = 78/83 (93.98%), Query Frame = 1

Query: 1  MALGGG-GPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMS 60
          MALGGG  PQRGSAAATASMRRRRTT  G SGGAAGTMLQFYTDDAPGLKISPNVVLVMS
Sbjct: 1  MALGGGTAPQRGSAAATASMRRRRTTGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMS 60

Query: 61 IGFIAFVAVLHVMGKLYFVRREA 83
          IGFIAFVA+LHVMGKLYFVR+EA
Sbjct: 61 IGFIAFVAILHVMGKLYFVRKEA 83

BLAST of CSPI03G07280 vs. TrEMBL
Match: V7AKD3_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_011G169200g PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 2.5e-30
Identity = 75/82 (91.46%), Postives = 76/82 (92.68%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGG  P RGSAAATA+MRRRRTT  G SGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGTAPPRGSAAATANMRRRRTTGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. TAIR10
Match: AT2G45070.1 (AT2G45070.1 Preprotein translocase Sec, Sec61-beta subunit protein)

HSP 1 Score: 122.1 bits (305), Expect = 1.6e-28
Identity = 67/81 (82.72%), Postives = 72/81 (88.89%), Query Frame = 1

Query: 3  LGGGGPQRGSAAATASMRRRRTTSS----GTSGGAAGTMLQFYTDDAPGLKISPNVVLVM 62
          +G G PQRGSAAATASMRRR+ TS     G SGGAAG+MLQFYTDDAPGLKISPNVVL+M
Sbjct: 2  VGSGAPQRGSAAATASMRRRKPTSGAGGGGASGGAAGSMLQFYTDDAPGLKISPNVVLIM 61

Query: 63 SIGFIAFVAVLHVMGKLYFVR 80
          SIGFIAFVAVLHVMGKLYFV+
Sbjct: 62 SIGFIAFVAVLHVMGKLYFVK 82

BLAST of CSPI03G07280 vs. TAIR10
Match: AT3G60540.1 (AT3G60540.1 Preprotein translocase Sec, Sec61-beta subunit protein)

HSP 1 Score: 120.6 bits (301), Expect = 4.7e-28
Identity = 65/78 (83.33%), Postives = 71/78 (91.03%), Query Frame = 1

Query: 3  LGGGGPQRGSAAATASMRRRRTTS-SGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSIG 62
          +GGG PQRGSAAATASMRRR+    S ++GG AGTMLQFYTDDAPGLKISPNVVL+MSIG
Sbjct: 2  VGGGAPQRGSAAATASMRRRKPAGGSSSAGGGAGTMLQFYTDDAPGLKISPNVVLIMSIG 61

Query: 63 FIAFVAVLHVMGKLYFVR 80
          FIAFVAVLHVMGKLYFV+
Sbjct: 62 FIAFVAVLHVMGKLYFVK 79

BLAST of CSPI03G07280 vs. TAIR10
Match: AT5G60460.1 (AT5G60460.1 Preprotein translocase Sec, Sec61-beta subunit protein)

HSP 1 Score: 81.6 bits (200), Expect = 2.4e-16
Identity = 49/75 (65.33%), Postives = 54/75 (72.00%), Query Frame = 1

Query: 10  RGSAAATASMRRRRTTSSG--TSGGAAGT------MLQFYTDDAPGLKISPNVVLVMSIG 69
           RGSAAATA MRRRR    G  +SGG +G       ML+FYTDDAPGLKISP VVL+MS+ 
Sbjct: 27  RGSAAATAGMRRRRLGGGGGVSSGGGSGVGSGSSNMLRFYTDDAPGLKISPTVVLIMSLC 86

Query: 70  FIAFVAVLHVMGKLY 77
           FI FV  LHV GKLY
Sbjct: 87  FIGFVTALHVFGKLY 101

BLAST of CSPI03G07280 vs. NCBI nr
Match: gi|778676662|ref|XP_011650635.1| (PREDICTED: protein transport protein Sec61 subunit beta [Cucumis sativus])

HSP 1 Score: 152.9 bits (385), Expect = 2.4e-34
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. NCBI nr
Match: gi|659074770|ref|XP_008437786.1| (PREDICTED: protein transport protein Sec61 subunit beta [Cucumis melo])

HSP 1 Score: 146.7 bits (369), Expect = 1.7e-32
Identity = 81/82 (98.78%), Postives = 81/82 (98.78%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MAL GGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MAL-GGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 81

BLAST of CSPI03G07280 vs. NCBI nr
Match: gi|502141511|ref|XP_004504530.1| (PREDICTED: protein transport protein Sec61 subunit beta-like [Cicer arietinum])

HSP 1 Score: 144.8 bits (364), Expect = 6.6e-32
Identity = 77/82 (93.90%), Postives = 78/82 (95.12%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGGGGPQRGSAAATASMRRR+T   G SGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGGGPQRGSAAATASMRRRKTAGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. NCBI nr
Match: gi|502085814|ref|XP_004488016.1| (PREDICTED: protein transport protein Sec61 subunit beta-like [Cicer arietinum])

HSP 1 Score: 144.4 bits (363), Expect = 8.7e-32
Identity = 76/82 (92.68%), Postives = 78/82 (95.12%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGGGGPQRGSAAATASMRRR+T   G SGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGGGPQRGSAAATASMRRRKTAGGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVA+LHVMGKLYFVRREA
Sbjct: 61 GFIAFVAILHVMGKLYFVRREA 82

BLAST of CSPI03G07280 vs. NCBI nr
Match: gi|388521563|gb|AFK48843.1| (unknown [Lotus japonicus])

HSP 1 Score: 142.9 bits (359), Expect = 2.5e-31
Identity = 77/82 (93.90%), Postives = 77/82 (93.90%), Query Frame = 1

Query: 1  MALGGGGPQRGSAAATASMRRRRTTSSGTSGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60
          MALGG  PQRGSAAA ASMRRRRTTS G SGGAAGTMLQFYTDDAPGLKISPNVVLVMSI
Sbjct: 1  MALGGAAPQRGSAAAAASMRRRRTTSGGASGGAAGTMLQFYTDDAPGLKISPNVVLVMSI 60

Query: 61 GFIAFVAVLHVMGKLYFVRREA 83
          GFIAFVAVLHVMGKLYFVRREA
Sbjct: 61 GFIAFVAVLHVMGKLYFVRREA 82

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SC61B_ARATH2.9e-2782.72Protein transport protein Sec61 subunit beta OS=Arabidopsis thaliana GN=At2g4507... [more]
SC61B_YARLI4.3e-0749.18Protein transport protein Sec61 subunit beta OS=Yarrowia lipolytica (strain CLIB... [more]
SC61B_DICDI4.8e-0649.06Protein transport protein Sec61 subunit beta OS=Dictyostelium discoideum GN=sec6... [more]
SC61B_CANLF6.2e-0643.59Protein transport protein Sec61 subunit beta OS=Canis lupus familiaris GN=SEC61B... [more]
SC61B_HUMAN6.2e-0643.59Protein transport protein Sec61 subunit beta OS=Homo sapiens GN=SEC61B PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0L6Y8_CUCSA1.7e-34100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G117430 PE=4 SV=1[more]
I3T8K1_LOTJA1.8e-3193.90Uncharacterized protein OS=Lotus japonicus PE=2 SV=1[more]
W9QQ35_9ROSA8.7e-3190.24Uncharacterized protein OS=Morus notabilis GN=L484_021777 PE=4 SV=1[more]
A0A151R1A8_CAJCA1.9e-3091.57Protein transport protein Sec61 subunit beta OS=Cajanus cajan GN=KK1_042494 PE=4... [more]
V7AKD3_PHAVU2.5e-3091.46Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_011G169200g PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G45070.11.6e-2882.72 Preprotein translocase Sec, Sec61-beta subunit protein[more]
AT3G60540.14.7e-2883.33 Preprotein translocase Sec, Sec61-beta subunit protein[more]
AT5G60460.12.4e-1665.33 Preprotein translocase Sec, Sec61-beta subunit protein[more]
Match NameE-valueIdentityDescription
gi|778676662|ref|XP_011650635.1|2.4e-34100.00PREDICTED: protein transport protein Sec61 subunit beta [Cucumis sativus][more]
gi|659074770|ref|XP_008437786.1|1.7e-3298.78PREDICTED: protein transport protein Sec61 subunit beta [Cucumis melo][more]
gi|502141511|ref|XP_004504530.1|6.6e-3293.90PREDICTED: protein transport protein Sec61 subunit beta-like [Cicer arietinum][more]
gi|502085814|ref|XP_004488016.1|8.7e-3292.68PREDICTED: protein transport protein Sec61 subunit beta-like [Cicer arietinum][more]
gi|388521563|gb|AFK48843.1|2.5e-3193.90unknown [Lotus japonicus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR016482SecG/Sec61-beta/Sbh
Vocabulary: Cellular Component
TermDefinition
GO:0005784Sec61 translocon complex
Vocabulary: Biological Process
TermDefinition
GO:0006886intracellular protein transport
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0005784 Sec61 translocon complex
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G07280.1CSPI03G07280.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016482Protein transport protein SecG/Sec61-beta/SbhPFAMPF03911Sec61_betacoord: 34..73
score: 1.6
NoneNo IPR availablePANTHERPTHR13509:SF2PREPROTEIN TRANSLOCASE SEC, SEC61-BETA SUBUNIT PROTEIN-RELATEDcoord: 1..81
score: 9.1