CSPI01G02550 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI01G02550
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	HNH endonuclease domain-containing protein
Location	Chr1 : 1618309 .. 1620671 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGGTGAGTCGGCCCGCTGAAAGAAGAGTCGAGGGGCAATGATCGGCGTAATTTAAGAAGAACGGAATGATCCAATGTCTTTAGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGTAGTATGATTCTTTAGTCGACGATTGGTTTCTGATTCCGATTTGAGAAGTTTTGCTTCTGCTGTTGCGTTCAGGGTTTAGTTTGGATTGTGATGAAATTTGTCGGCGACTGTTTGCAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGGTACTTCTTCATCCTGCTCCAATTCTTTCTTTTGGTATTCATATTCTTCTATTTTTCATATCCATTTATTGGCTGGTAATCTATGAGCTGCCGATACCAAGTTACATAAAATCAGATTTACATATATCTCACTTTCTAGTTTCCGTACTGCCTATAATTTTTCGCAAATTCTTTCTGCCGTACTATTCAATGCAAGAACTTCTCAAATTCATGCTTGTTTAAGCTACGAGTAAACCATCCGTTTCGAATACCAAAGTAGTTCACCCTACTGTTGTTTTGTTATTGCAATTTCTCCCAGTAAAATATAACTTTTGGCATTGATCTCTTTTTCTTAGCTGCCCCATATGGCCAAATGTTCATCAGCTTTTACGTAATCATATGGTTTGTTGCTCAAAATCTGTAGTTTCTGTAAAGTTGCTCAGCCATACCGTGAGACAATTGATTGGCTGTTGTGCTTTTATTTAACTTTTGTTGGTACTTGATGACAATTTCGGGGAAAGTGATCTAGAAACATCATTACAGTTTCAGGAAAGTGCTAGAAAGTGAAGTTTCAATTGTTTTAGAAAGATCTGTTGTCCTGTCCCATATTAGCTGTCATTTCTGGTGGTAGTTTCTGATTAAAAATTTAAAACAAACGAGTTCTTGTATTTTAGGTCATCTGAAACTTTAAGTGATGAATCTCCATAGGCTGCTGTCTAGAACTTCGAAGTATAAACTCACCTCCTTAAGGTTATGAATTCTAATATCTTATGAAAATTTCATTGAATGATGTAGTATAATTAAATTGCTGTGCATAAGCAGACAAGAGCACTCTCTGTTTATAAGATCGTCACCTCTTAGTTCATTGTGCTCCCTTTGAAACCTGCTTTCATGCATCTCAAGAAAGTCACTTTGTTACCAACCCACCAAAGAAACTCCTTTGACATTTTTTTTTTTGTATAGAAACTATTTTCTCATGTAAGGGCGTACAAAAAAGAAAAAAAAACAAGCCTGCACCTACTGAATAGAATGTGCATAAAGAGTAATTACAAAATGTGTTTGAAATGGAAGGCTAAACATCAATTGGATATCTCTCCTCCACCTCTCCCTAACCACAGTTATTTCTCTACCTCATCAATCTCACATTTAATCGCACACCCAACATCTCAAAGCAAGCAATCATTCTCACCAAAAGACTGATGGAGAACTCGCTCCACTATTGAACCACTAACGCTTGACCATCTCATGGTAGTCTATATTATAGAATCTGATTAAAACAGTTGATGCGTCAAGGTTTTTTCGCGTCTATCTCACATCCATATATGATGTATTTGGGCTTCATAATTCATTGCAGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAACAACAAAGAAGACTTGAAACTAAAGCATGTTTGCCCCATCTATAGTTTAATACGATTGATAAACATCATTTTAAGATTACATCAATTAGAATTGTGTGGACCTCCATTACTTGATTAGATCAATATGAATCAACATCGACCATCATTTTTTTTTTATTATTAGGGTTTGCTTTCAAATATACTAAAATATTTATGCC

mRNA sequence

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAA

Coding sequence (CDS)

ATGGGCCTTCTCCAATTTCCCCGTTCGGCCCATTCGGATTGTGGGCGTTTCTTCCATTTTTGTCTGCTGGACGGCGTCCCGACTCCGATGGAAGCTACTTCCAGTTCGCCGTCACCGACTCGTTCCCGCGGCGGGCAAAGAGATAGTGGCTCCGGAAGTGGGGACGAACGGCCAAGATTTTTCGACGCAAAAGCGAAAGCTAGTTGTTGGGCTAAGGCGGATGTTGTCCCTGGGCGGCATCCCGAGCGTTGGCGTAAGGACGCCGCTGGTAATGTCGTCTGTAAGCGCTTCTGTAACTGCCAGGGCTGCCTCTGTTTTGAGTATGATCACATTGTCCCTTACTCTAAAGGAGGCGAATCCACGGCGGATAATTGCCAGATTCTTCAGACGAGAGTAAACAGATTCAAATCGAATAAAGACGATGTTGATACGTCTGAGTTGAAAAGCTATTCGTGTGATGTCAAGTTTACTGATAAGGAGCTTGACATAATTGAAATGGCTGTTTACGGAGATGTAATCCGGCCAGGGAACCAATGTCGTTGTAGAACAGTTTCTGAAGTGCTTGGGCAATATAAATCTAAAGACCGTTTGGCCCCCTGCAAATTGCCATATAATGAAGAAGGTTCTTTGTAA

Homology

[Close]

BLAST of CSPI01G02550 vs. TrEMBL
Match: A0A0A0LUK3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G011480 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 1.4e-122
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 1


Query: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60
           MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF
Sbjct: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60

Query: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120
           FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES
Sbjct: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120

Query: 121 TADNCQILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180
           TADNCQILQTRVNRFKSNKDDVDTSELK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCR
Sbjct: 121 TADNCQILQTRVNRFKSNKDDVDTSELKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180

Query: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 211
           CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL
Sbjct: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 210

[Close]

BLAST of CSPI01G02550 vs. TrEMBL
Match: M5WAX4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012267mg PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 1.8e-80
Identity = 142/174 (81.61%), Postives = 158/174 (90.80%), Query Frame = 1


Query: 32  ATSSSPSPTRSRGGQRD--SGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 91
           ++ SS SP RSRGG  +  S  G G++RPRFFD+KAK++CWAKADVVPGRHPERWRKDAA
Sbjct: 4   SSPSSSSPHRSRGGGDNGTSSKGKGEDRPRFFDSKAKSNCWAKADVVPGRHPERWRKDAA 63

Query: 92  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 151
           GNVVCKRFCNCQGCLCFEYDHIVP+SKGGEST DNCQILQTRVNRFKS+KD +D ++LK 
Sbjct: 64  GNVVCKRFCNCQGCLCFEYDHIVPFSKGGESTEDNCQILQTRVNRFKSDKDQIDKTQLKG 123

Query: 152 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 204
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTV+E+LGQYKSKD+ A CKLP
Sbjct: 124 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVAEMLGQYKSKDKTAACKLP 177

[Close]

BLAST of CSPI01G02550 vs. TrEMBL
Match: A0A0D2TTQ0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_008G195700 PE=4 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 5.8e-79
Identity = 138/174 (79.31%), Postives = 155/174 (89.08%), Query Frame = 1


Query: 34  SSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAAGNVV 93
           SSSPSP RSR       S   +ERPRFFD+KAK+ CWA A+ VPGRHPERWRKDAAGN+V
Sbjct: 2   SSSPSPRRSRS------SNGEEERPRFFDSKAKSKCWANAETVPGRHPERWRKDAAGNIV 61

Query: 94  CKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKSYSCD 153
           CKRFCNCQGCLCFEYDHIVP+SKGGESTA+NCQILQTRVNRFKSNK+D+DT+ LK YSC+
Sbjct: 62  CKRFCNCQGCLCFEYDHIVPFSKGGESTAENCQILQTRVNRFKSNKEDLDTTRLKGYSCE 121

Query: 154 VKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           V+FTDKELDIIEMAVYGDVIRPGNQCRCRT++E+LGQYKSKD LA CKLP ++E
Sbjct: 122 VQFTDKELDIIEMAVYGDVIRPGNQCRCRTIAEMLGQYKSKDNLAACKLPLDKE 169

[Close]

BLAST of CSPI01G02550 vs. TrEMBL
Match: A0A0B0MHS4_GOSAR (Beta-galactosidase 17-like protein OS=Gossypium arboreum GN=F383_18550 PE=4 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.7e-78
Identity = 137/174 (78.74%), Postives = 155/174 (89.08%), Query Frame = 1


Query: 34  SSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAAGNVV 93
           SSSPSP RSR       S   +ERPRFFD+KAK+ CWA A+ VPGRHPERWRKDAAGN+V
Sbjct: 2   SSSPSPRRSRS------SNGEEERPRFFDSKAKSKCWANAETVPGRHPERWRKDAAGNIV 61

Query: 94  CKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKSYSCD 153
           CKRFCNCQGCLCFEYDHIVP+SKGGESTA+NCQILQTRVNRFKSNK+D+DT+ LK YSC+
Sbjct: 62  CKRFCNCQGCLCFEYDHIVPFSKGGESTAENCQILQTRVNRFKSNKEDLDTTRLKGYSCE 121

Query: 154 VKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           V+FT+KELDIIEMAVYGDVIRPGNQCRCRT++E+LGQYKSKD LA CKLP ++E
Sbjct: 122 VQFTEKELDIIEMAVYGDVIRPGNQCRCRTIAEMLGQYKSKDNLAACKLPLDKE 169

[Close]

BLAST of CSPI01G02550 vs. TrEMBL
Match: V4TQC1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022490mg PE=4 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.4e-77
Identity = 135/176 (76.70%), Postives = 156/176 (88.64%), Query Frame = 1


Query: 32  ATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAAGN 91
           ++SSS  P RSR     + + S +ERPR+FD KAK+ CWAKA+ VPGRHPERWRKDAAGN
Sbjct: 7   SSSSSSLPNRSRSS---NSNNSNEERPRYFDGKAKSMCWAKAETVPGRHPERWRKDAAGN 66

Query: 92  VVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKSYS 151
           +VCKRFCNC GCLCFEYDHIVP+SKGGESTADNCQILQTRVNRFKS+K+ VD ++LK +S
Sbjct: 67  IVCKRFCNCHGCLCFEYDHIVPFSKGGESTADNCQILQTRVNRFKSDKEQVDATKLKGFS 126

Query: 152 CDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           CDVKFTDKELDIIEMAVYGDVIRPGNQCRC+TV+E+LGQ+KSKDR+A CKLPY+ E
Sbjct: 127 CDVKFTDKELDIIEMAVYGDVIRPGNQCRCKTVAEMLGQFKSKDRMAACKLPYDGE 179

[Close]

BLAST of CSPI01G02550 vs. TAIR10
Match: AT1G18680.1 (AT1G18680.1 HNH endonuclease domain-containing protein)

HSP 1 Score: 265.0 bits (676), Expect = 3.9e-71
Identity = 120/176 (68.18%), Postives = 142/176 (80.68%), Query Frame = 1


Query: 28  TPMEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKD 87
           T     ++ P P+      R +   +G+ERPRFFD KAK  CWA AD+VPGRHPERWRKD
Sbjct: 10  TNSTTAAAVPPPSPPSSPSRSNVKSNGEERPRFFDGKAKNKCWANADIVPGRHPERWRKD 69

Query: 88  AAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSEL 147
            AGN+VCKRF NC GCLCFEYDHIVPYSKGGES A+NCQILQTRVNRFKS +++VD + L
Sbjct: 70  VAGNIVCKRFGNCNGCLCFEYDHIVPYSKGGESIAENCQILQTRVNRFKSAQENVDATTL 129

Query: 148 KSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLP 204
           KSYSC ++FTDKELD+IEMAVYGDV+RPG +CRC+TV+E+LGQ KSKD  A C+LP
Sbjct: 130 KSYSCGLQFTDKELDVIEMAVYGDVLRPGKECRCKTVAELLGQSKSKDGKAACELP 185

[Close]

BLAST of CSPI01G02550 vs. TAIR10
Match: AT3G47490.1 (AT3G47490.1 HNH endonuclease)

HSP 1 Score: 144.1 bits (362), Expect = 1.0e-34
Identity = 75/176 (42.61%), Postives = 96/176 (54.55%), Query Frame = 1


Query: 27  PTPMEATSSSPSPTRSRGGQRDS------------------GSGSGDERP--RFFDAKAK 86
           P+      SS SP R+ G  R                    GSG GD  P  R F    K
Sbjct: 57  PSKTRPELSSGSPNRNTGEARGGKVKTSATLLDREEMGLFPGSGYGDPNPEPRSFPYSVK 116

Query: 87  ASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQ 146
             CW KA+ + GR PERWR+D  GN+V ++   C GCLC +YDHIVPYSKGG+ST +NCQ
Sbjct: 117 QQCWEKAEKIKGRDPERWRRDHLGNIVFRKLVGCPGCLCHDYDHIVPYSKGGKSTLENCQ 176

Query: 147 ILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCR 183
           +LQ +VNR K NK D+  SEL   S   +   +++D+IE+  YG+V R      CR
Sbjct: 177 VLQAKVNRSKGNKTDISRSELIQRSSYCRVAGRDMDLIELTAYGNVQRAPTSSGCR 232

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: gi|700208600|gb|KGN63696.1| (hypothetical protein Csa_1G011480 [Cucumis sativus])

HSP 1 Score: 446.8 bits (1148), Expect = 2.1e-122
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 1


Query: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60
           MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF
Sbjct: 1   MGLLQFPRSAHSDCGRFFHFCLLDGVPTPMEATSSSPSPTRSRGGQRDSGSGSGDERPRF 60

Query: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120
           FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES
Sbjct: 61  FDAKAKASCWAKADVVPGRHPERWRKDAAGNVVCKRFCNCQGCLCFEYDHIVPYSKGGES 120

Query: 121 TADNCQILQTRVNRFKSNKDDVDTSELKSYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180
           TADNCQILQTRVNRFKSNKDDVDTSELK YSCDVKFTDKELDIIEMAVYGDVIRPGNQCR
Sbjct: 121 TADNCQILQTRVNRFKSNKDDVDTSELKGYSCDVKFTDKELDIIEMAVYGDVIRPGNQCR 180

Query: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 211
           CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL
Sbjct: 181 CRTVSEVLGQYKSKDRLAPCKLPYNEEGSL 210

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: gi|449440818|ref|XP_004138181.1| (PREDICTED: uncharacterized protein LOC101204599 isoform X2 [Cucumis sativus])

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-102
Identity = 180/181 (99.45%), Postives = 180/181 (99.45%), Query Frame = 1


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS 209
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEEGS 180

Query: 210 L 211
           L
Sbjct: 181 L 181

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: gi|778655999|ref|XP_011660190.1| (PREDICTED: uncharacterized protein LOC101204599 isoform X1 [Cucumis sativus])

HSP 1 Score: 375.6 bits (963), Expect = 5.9e-101
Identity = 180/185 (97.30%), Postives = 180/185 (97.30%), Query Frame = 1


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFT----DKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYN 209
           YSCDVKFT    DKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYN
Sbjct: 121 YSCDVKFTGFLADKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYN 180

Query: 210 EEGSL 211
           EEGSL
Sbjct: 181 EEGSL 185

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: gi|659105999|ref|XP_008453224.1| (PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo])

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-100
Identity = 175/178 (98.31%), Postives = 177/178 (99.44%), Query Frame = 1


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYNEE 208
           YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYNE+
Sbjct: 121 YSCDVKFTDKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYNED 178

[Close]

BLAST of CSPI01G02550 vs. NCBI nr
Match: gi|659105995|ref|XP_008453223.1| (PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo])

HSP 1 Score: 367.5 bits (942), Expect = 1.6e-98
Identity = 175/182 (96.15%), Postives = 177/182 (97.25%), Query Frame = 1


Query: 30  MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 89
           MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA
Sbjct: 1   MEATSSSPSPTRSRGGQRDSGSGSGDERPRFFDAKAKASCWAKADVVPGRHPERWRKDAA 60

Query: 90  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKS 149
           GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELK 
Sbjct: 61  GNVVCKRFCNCQGCLCFEYDHIVPYSKGGESTADNCQILQTRVNRFKSNKDDVDTSELKG 120

Query: 150 YSCDVKFT----DKELDIIEMAVYGDVIRPGNQCRCRTVSEVLGQYKSKDRLAPCKLPYN 208
           YSCDVKFT    DKELDIIEMAVYGDVIRPGNQCRCRTVSE+LGQYKSKDRLAPCKLPYN
Sbjct: 121 YSCDVKFTGFLADKELDIIEMAVYGDVIRPGNQCRCRTVSEMLGQYKSKDRLAPCKLPYN 180

The following BLAST results are available for this feature:

BLAST of CSPI01G02550 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of CSPI01G02550 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LUK3_CUCSA	1.4e-122	99.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G011480 PE=4 SV=1	[more]
M5WAX4_PRUPE	1.8e-80	81.61	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012267mg PE=4 SV=1	[more]
A0A0D2TTQ0_GOSRA	5.8e-79	79.31	Uncharacterized protein OS=Gossypium raimondii GN=B456_008G195700 PE=4 SV=1	[more]
A0A0B0MHS4_GOSAR	1.7e-78	78.74	Beta-galactosidase 17-like protein OS=Gossypium arboreum GN=F383_18550 PE=4 SV=1	[more]
V4TQC1_9ROSI	2.4e-77	76.70	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022490mg PE=4 SV=1	[more]

BLAST of CSPI01G02550 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G18680.1	3.9e-71	68.18	HNH endonuclease domain-containing protein	[more]
AT3G47490.1	1.0e-34	42.61	HNH endonuclease	[more]

BLAST of CSPI01G02550 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700208600\|gb\|KGN63696.1\|	2.1e-122	99.52	hypothetical protein Csa_1G011480 [Cucumis sativus]	[more]
gi\|449440818\|ref\|XP_004138181.1\|	1.1e-102	99.45	PREDICTED: uncharacterized protein LOC101204599 isoform X2 [Cucumis sativus]	[more]
gi\|778655999\|ref\|XP_011660190.1\|	5.9e-101	97.30	PREDICTED: uncharacterized protein LOC101204599 isoform X1 [Cucumis sativus]	[more]
gi\|659105999\|ref\|XP_008453224.1\|	2.9e-100	98.31	PREDICTED: uncharacterized protein LOC103494011 isoform X2 [Cucumis melo]	[more]
gi\|659105995\|ref\|XP_008453223.1\|	1.6e-98	96.15	PREDICTED: uncharacterized protein LOC103494011 isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002711	HNH
IPR003615	HNH_nuc

Vocabulary: **Molecular Function**
Term	Definition
GO:0003676	nucleic acid binding
GO:0004519	endonuclease activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0003676	nucleic acid binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI01G02550.1	CSPI01G02550.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002711	HNH endonuclease	PFAM	PF01844	HNH	coord: 94..128 score: 5.
IPR003615	HNH nuclease	SMART	SM00507	HNH_5	coord: 80..134 score: 0.
None	No IPR available	PANTHER	PTHR33427	FAMILY NOT NAMED	coord: 56..207 score: 9.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI01G02550	Cla019088	Watermelon (97103) v1	cpiwmB099
CSPI01G02550	Csa1G011480	Cucumber (Chinese Long) v2	cpicuB000
CSPI01G02550	MELO3C017197	Melon (DHL92) v3.5.1	cpimeB055
CSPI01G02550	ClCG06G015940	Watermelon (Charleston Gray)	cpiwcgB083
CSPI01G02550	Lsi06G014430	Bottle gourd (USVL1VR-Ls)	cpilsiB070
CSPI01G02550	MELO3C017197.2	Melon (DHL92) v3.6.1	cpimedB049
CSPI01G02550	CsaV3_1G002610	Cucumber (Chinese Long) v3	cpicucB001
CSPI01G02550	Cla97C06G125520	Watermelon (97103) v2	cpiwmbB079
CSPI01G02550	Bhi02G000198	Wax gourd	cpiwgoB098
CSPI01G02550	Cucsa.197940	Cucumber (Gy14) v1	cgycpiB310
CSPI01G02550	CmaCh11G001220	Cucurbita maxima (Rimu)	cmacpiB098
CSPI01G02550	CmoCh11G001900	Cucurbita moschata (Rifu)	cmocpiB079
CSPI01G02550	CsGy1G002580	Cucumber (Gy14) v2	cgybcpiB001

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI01G02550	Wax gourd	cpiwgoB053
CSPI01G02550	Wax gourd	cpiwgoB071
CSPI01G02550	Wild cucumber (PI 183967)	cpicpiB000
CSPI01G02550	Wild cucumber (PI 183967)	cpicpiB029
CSPI01G02550	Cucumber (Gy14) v1	cgycpiB138
CSPI01G02550	Cucumber (Gy14) v1	cgycpiB427
CSPI01G02550	Cucurbita maxima (Rimu)	cmacpiB049
CSPI01G02550	Cucurbita maxima (Rimu)	cmacpiB226
CSPI01G02550	Cucurbita maxima (Rimu)	cmacpiB432
CSPI01G02550	Cucurbita maxima (Rimu)	cmacpiB693
CSPI01G02550	Cucurbita moschata (Rifu)	cmocpiB040
CSPI01G02550	Cucurbita moschata (Rifu)	cmocpiB212
CSPI01G02550	Cucurbita moschata (Rifu)	cmocpiB422
CSPI01G02550	Cucumber (Chinese Long) v2	cpicuB005
CSPI01G02550	Cucumber (Chinese Long) v2	cpicuB037
CSPI01G02550	Melon (DHL92) v3.5.1	cpimeB005
CSPI01G02550	Melon (DHL92) v3.5.1	cpimeB026
CSPI01G02550	Watermelon (Charleston Gray)	cpiwcgB060
CSPI01G02550	Watermelon (Charleston Gray)	cpiwcgB066
CSPI01G02550	Watermelon (97103) v1	cpiwmB039
CSPI01G02550	Watermelon (97103) v1	cpiwmB067
CSPI01G02550	Cucurbita pepo (Zucchini)	cpecpiB361
CSPI01G02550	Cucurbita pepo (Zucchini)	cpecpiB512
CSPI01G02550	Cucurbita pepo (Zucchini)	cpecpiB538
CSPI01G02550	Cucurbita pepo (Zucchini)	cpecpiB656
CSPI01G02550	Bottle gourd (USVL1VR-Ls)	cpilsiB024
CSPI01G02550	Bottle gourd (USVL1VR-Ls)	cpilsiB055
CSPI01G02550	Melon (DHL92) v3.6.1	cpimedB005
CSPI01G02550	Melon (DHL92) v3.6.1	cpimedB028
CSPI01G02550	Cucumber (Gy14) v2	cgybcpiB004
CSPI01G02550	Silver-seed gourd	carcpiB0057
CSPI01G02550	Silver-seed gourd	carcpiB0420
CSPI01G02550	Silver-seed gourd	carcpiB0499
CSPI01G02550	Cucumber (Chinese Long) v3	cpicucB003
CSPI01G02550	Cucumber (Chinese Long) v3	cpicucB044
CSPI01G02550	Watermelon (97103) v2	cpiwmbB056
CSPI01G02550	Watermelon (97103) v2	cpiwmbB063