Bhi08G000646 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi08G000646
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cysteine proteinase inhibitor
Location	chr8 : 25579882 .. 25580970 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGAAGTCGATTAATAACTATACGTATTATTTTTGCTATTTTTACAAATAGTTTGACATTATTTTCTAGCCGTGAAAATCTCTCTTTATTTGAAGGGCACTAAAATGAGACTTGTGAATAAGGGTTGGCTAGGAAGAAAGGCGTTCCAATTCTGTGCAAGTGGTCCGGTTTCCGCGTCCTTCGAAAATTCCTATAAATTGCCCCCTAAAACCACTCCTCCAAGCAATTGCCCTAAATAATTTCCCTTCATTCATTCATTCCTTCCCTCAACGGTTACAATGAATTCCCGCTCCGCTTCTCTTTTCCTCATCCTTCTTCTCCCCCTCCTCTCCGCCGTCGTCGCTCGGAAGGGCGCTCTGCTCGGCGGCTGGAAGAAGATCGACGACGTTAATGATCCGCACGTTCAAGAGATCGCAATGTTCGCGGTGACCGAGTACAACAAGCAATCCAAAGGCGTAACGATCGAATTCAAGTCCGTCGTCAGCGGCGAAACGCAGGTCGTTTCTGGAATCAACTACCGCCTCGTTATCGACGCAAAGAGAGGCGAATCGGTTGGCAAATATCGGGCGATGGTCTGGGAGAAGCCATGGGAGAATTCCAAGAAACTTACGTCCTTCAAGCCTTTTGCCTAATTTCACCAGTAAAAGCCCTAATTTGCCTTCTTATTGTGCTGTTTGTTGTGTCAGTATTTGGTAATTAGGTAAAATAAATTTGAATTTCCTAGTAATAATTTCTGGAGAAATCGTTTGTGGATTCTGTTTGGTTATCAGATGGATCTCTGTTTTCTTCGTAATCATAATTATTTCTGTTAGTTTGTAATTTTATATTAAAAATATTTTGTTATAAAGGTCTCAGATACTATTGGGATTTCTTATCTTCATAAAAGGGAAATGAATAATAAATTATAGATACTTTATCTATGGAAATGCTCTTGTACATGCATTAGTGAATTAAATACTATTTCACTTGCAAATTGGGTTGGAAAAGACAAAAGCTATAAATTTCTATGGATAATTGATCATTCAATCAAAGTTATCAAACCACTTTTCTTTTTTGGATAAACTATTGTACTAGATATTTTTGGGAGG

mRNA sequence

ATAGAAGTCGATTAATAACTATACGTATTATTTTTGCTATTTTTACAAATAGTTTGACATTATTTTCTAGCCGTGAAAATCTCTCTTTATTTGAAGGGCACTAAAATGAGACTTGTGAATAAGGGTTGGCTAGGAAGAAAGGCGTTCCAATTCTGTGCAAGTGGTCCGGTTTCCGCGTCCTTCGAAAATTCCTATAAATTGCCCCCTAAAACCACTCCTCCAAGCAATTGCCCTAAATAATTTCCCTTCATTCATTCATTCCTTCCCTCAACGGTTACAATGAATTCCCGCTCCGCTTCTCTTTTCCTCATCCTTCTTCTCCCCCTCCTCTCCGCCGTCGTCGCTCGGAAGGGCGCTCTGCTCGGCGGCTGGAAGAAGATCGACGACGTTAATGATCCGCACGTTCAAGAGATCGCAATGTTCGCGGTGACCGAGTACAACAAGCAATCCAAAGGCGTAACGATCGAATTCAAGTCCGTCGTCAGCGGCGAAACGCAGGTCGTTTCTGGAATCAACTACCGCCTCGTTATCGACGCAAAGAGAGGCGAATCGGTTGGCAAATATCGGGCGATGGTCTGGGAGAAGCCATGGGAGAATTCCAAGAAACTTACGTCCTTCAAGCCTTTTGCCTAATTTCACCAGTAAAAGCCCTAATTTGCCTTCTTATTGTGCTGTTTGTTGTGTCAGTATTTGGTAATTAGGTAAAATAAATTTGAATTTCCTAGTAATAATTTCTGGAGAAATCGTTTGTGGATTCTGTTTGGTTATCAGATGGATCTCTGTTTTCTTCGTAATCATAATTATTTCTGTTAGTTTGTAATTTTATATTAAAAATATTTTGTTATAAAGGTCTCAGATACTATTGGGATTTCTTATCTTCATAAAAGGGAAATGAATAATAAATTATAGATACTTTATCTATGGAAATGCTCTTGTACATGCATTAGTGAATTAAATACTATTTCACTTGCAAATTGGGTTGGAAAAGACAAAAGCTATAAATTTCTATGGATAATTGATCATTCAATCAAAGTTATCAAACCACTTTTCTTTTTTGGATAAACTATTGTACTAGATATTTTTGGGAGG

Coding sequence (CDS)

ATGAATTCCCGCTCCGCTTCTCTTTTCCTCATCCTTCTTCTCCCCCTCCTCTCCGCCGTCGTCGCTCGGAAGGGCGCTCTGCTCGGCGGCTGGAAGAAGATCGACGACGTTAATGATCCGCACGTTCAAGAGATCGCAATGTTCGCGGTGACCGAGTACAACAAGCAATCCAAAGGCGTAACGATCGAATTCAAGTCCGTCGTCAGCGGCGAAACGCAGGTCGTTTCTGGAATCAACTACCGCCTCGTTATCGACGCAAAGAGAGGCGAATCGGTTGGCAAATATCGGGCGATGGTCTGGGAGAAGCCATGGGAGAATTCCAAGAAACTTACGTCCTTCAAGCCTTTTGCCTAA

Protein sequence

MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA

Homology

[Close]

BLAST of Bhi08G000646 vs. Swiss-Prot
Match: sp|Q6TPK4|CYT1_ACTDE (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 2.8e-23
Identity = 59/115 (51.30%), Postives = 79/115 (68.70%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVV-ARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKG 60
           M  +  SL L LLL L +AVV  RK    GGW+ I+ +N   VQ++A FAV+E+NKQ+  
Sbjct: 1   MVPKPLSLLLFLLLALSAAVVGGRKLVAAGGWRPIESLNSAEVQDVAQFAVSEHNKQAND 60

Query: 61  VTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFK 115
             ++++SVV G TQVV+G NYRLVI AK G  VG Y A+VW+KPW + + LTSF+
Sbjct: 61  -ELQYQSVVRGYTQVVAGTNYRLVIAAKDGAVVGNYEAVVWDKPWMHFRNLTSFR 114

[Close]

BLAST of Bhi08G000646 vs. Swiss-Prot
Match: sp|Q41916|CYT5_ARATH (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 1.1e-22
Identity = 58/116 (50.00%), Postives = 78/116 (67.24%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M S+   L L+ L+ +L  + A   A +GGW  I +V DP V EI  FAV+EYNK+S+  
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYASAAARVGGWSPISNVTDPQVVEIGEFAVSEYNKRSES- 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGK-YRAMVWEKPWENSKKLTSFKP 116
            ++F++VVSGETQVVSG NYRL + A  G+ V K Y A+VW+KPW   + LTSF+P
Sbjct: 61  GLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Bhi08G000646 vs. Swiss-Prot
Match: sp|Q10Q46|CYT6_ORYSJ (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.3e-17
Identity = 46/100 (46.00%), Postives = 63/100 (63.00%), Query Frame = 0


Query: 16  LLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGVTIEFKSVVSGETQVV 75
           LL A V    AL  GW  I +++DPH+QE+  +A+TE N+ S    + F  V  GE QVV
Sbjct: 8   LLLAAVCAAAALPRGWSPIKNIDDPHIQELGRWAITENNRVSPSDELTFHRVTGGEQQVV 67

Query: 76  SGINYRLVIDAKR--GESVGKYRAMVWEKPWENSKKLTSF 114
           SG+NYRL I+A    G+  G Y A+V+E+ W N++KL SF
Sbjct: 68  SGMNYRLEIEAASGGGDVTGSYGAVVFEQEWSNTRKLISF 107

[Close]

BLAST of Bhi08G000646 vs. Swiss-Prot
Match: sp|Q10J94|CYT8_ORYSJ (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.9e-17
Identity = 52/117 (44.44%), Postives = 71/117 (60.68%), Query Frame = 0


Query: 8   LFLILLLPLLSAVVAR------KGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGVT 67
           L L LLL + +A  A+       G L+GGW  I DV DPH+QE+  +AV  +   S    
Sbjct: 6   LLLALLLAVSAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSSD-G 65

Query: 68  IEFKSVVSGETQVVSGINYRLVIDAK-RGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
           + F+ V SGE QVVSG+NYRLV+ A     +   Y A+V+E+ W N+++LTSFKP A
Sbjct: 66  LRFRRVTSGEQQVVSGMNYRLVVSASDPAGATASYVAVVYEQSWTNTRQLTSFKPAA 121

[Close]

BLAST of Bhi08G000646 vs. Swiss-Prot
Match: sp|P09229|CYT1_ORYSJ (Cysteine proteinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0803200 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 2.8e-15
Identity = 40/91 (43.96%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 25  GALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGVTIEFKSVVSGETQVVSGINYRLVI 84
           G +LGG + + + ND H+ ++A FAVTE+NK++  + +EF+ +VS + QVV+G  Y   I
Sbjct: 44  GPVLGGVEPVGNENDLHLVDLARFAVTEHNKKANSL-LEFEKLVSVKQQVVAGTLYYFTI 103

Query: 85  DAKRGESVGKYRAMVWEKPWENSKKLTSFKP 116
           + K G++   Y A VWEKPW + K+L  FKP
Sbjct: 104 EVKEGDAKKLYEAKVWEKPWMDFKELQEFKP 133

[Close]

BLAST of Bhi08G000646 vs. TAIR10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 107.5 bits (267), Expect = 5.8e-24
Identity = 58/116 (50.00%), Postives = 78/116 (67.24%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M S+   L L+ L+ +L  + A   A +GGW  I +V DP V EI  FAV+EYNK+S+  
Sbjct: 1   MTSKVVFLLLLSLVVVLLPLYASAAARVGGWSPISNVTDPQVVEIGEFAVSEYNKRSES- 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGK-YRAMVWEKPWENSKKLTSFKP 116
            ++F++VVSGETQVVSG NYRL + A  G+ V K Y A+VW+KPW   + LTSF+P
Sbjct: 61  GLKFETVVSGETQVVSGTNYRLKVAANDGDGVSKNYLAIVWDKPWMKFRNLTSFEP 115

[Close]

BLAST of Bhi08G000646 vs. TAIR10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein)

HSP 1 Score: 75.5 bits (184), Expect = 2.5e-14
Identity = 52/116 (44.83%), Postives = 67/116 (57.76%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVA-RKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKG 60
           M  +S     ++LLPL+S V     G  LG  K I +V+DP V  +A +A+ E+NK+SK 
Sbjct: 1   MMMKSLICLSLILLPLVSVVEGLGGGGGLGSRKPIKNVSDPDVVAVAKYAIEEHNKESKE 60

Query: 61  VTIEFKSVVSGETQVVSGINYRLVIDAK-RGESVGKYRAMVWEKPWENSKKLTSFK 115
             + F  VV G TQVVSG  Y L I AK  G  +  Y A+V EK W +SK L SFK
Sbjct: 61  KLV-FVKVVEGTTQVVSGTKYDLKIAAKDGGGKIKNYEAVVVEKLWLHSKSLESFK 115

[Close]

BLAST of Bhi08G000646 vs. TAIR10
Match: AT5G12140.1 (cystatin-1)

HSP 1 Score: 72.8 bits (177), Expect = 1.6e-13
Identity = 38/90 (42.22%), Postives = 59/90 (65.56%), Query Frame = 0


Query: 25  GALLGGWKKID-DVNDPHVQEIAMFAVTEYNKQSKGVTIEFKSVVSGETQVVSGINYRLV 84
           G ++GG + ID + ND  V+ +A FAV E+NK ++ +T+E+K ++  +TQVV+G  + L 
Sbjct: 7   GTIVGGVRDIDANANDLQVESLARFAVDEHNK-NENLTLEYKRLLGAKTQVVAGTMHHLT 66

Query: 85  IDAKRGESVGKYRAMVWEKPWENSKKLTSF 114
           ++   GE+   Y A V EK WEN K+L SF
Sbjct: 67  VEVADGETNKVYEAKVLEKAWENLKQLESF 95

[Close]

BLAST of Bhi08G000646 vs. TAIR10
Match: AT2G40880.1 (cystatin A)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-13
Identity = 41/122 (33.61%), Postives = 64/122 (52.46%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVAR-------KGALLGGWKKI-DDVNDPHVQEIAMFAVTE 60
           M S++  +  +LL   +   + R       K  +LGG   +  + N   ++ +A FA+ E
Sbjct: 1   MESKTFWIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQE 60

Query: 61  YNKQSKGVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTS 115
           +NKQ   + +EFK +V    QVV+G  Y L ++AK G+    + A VW KPW N K+L  
Sbjct: 61  HNKQQNKI-LEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQE 120

[Close]

BLAST of Bhi08G000646 vs. TAIR10
Match: AT5G05110.1 (Cystatin/monellin family protein)

HSP 1 Score: 57.8 bits (138), Expect = 5.3e-09
Identity = 35/91 (38.46%), Postives = 48/91 (52.75%), Query Frame = 0


Query: 28  LGGWKKIDDVND----PHVQEIAMFAVTEYNKQSKGVTIEFKSVVSGETQVVSGINYRLV 87
           LGG+   D  ND      + +IA+FAV E+N++   V +E   V+    QVV+G  YRL 
Sbjct: 45  LGGFS--DSKNDWNGGKEIDDIALFAVQEHNRRENAV-LELARVLKATEQVVAGKLYRLT 104

Query: 88  IDAKRGESVGKYRAMVWEKPWENSKKLTSFK 115
           ++         Y A VW KPW N K+L  FK
Sbjct: 105 LEVIEAGEKKIYEAKVWVKPWMNFKQLQEFK 132

[Close]

BLAST of Bhi08G000646 vs. TrEMBL
Match: tr|A0A0A0LTF5|A0A0A0LTF5_CUCSA (Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G183580 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.7e-41
Identity = 89/117 (76.07%), Postives = 100/117 (85.47%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M SRSAS  L+LLLPL++A  ARKG++LGGW KI D+ DPHV+EI  FAV EYNKQSKGV
Sbjct: 1   MISRSASPLLLLLLPLIAA--ARKGSMLGGWSKIPDLKDPHVEEIGKFAVAEYNKQSKGV 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
            IEFKSVVSGETQVV+G NYRL+IDAKRGES+ KY A+VWEKPWEN KKLTSFKP A
Sbjct: 61  AIEFKSVVSGETQVVAGTNYRLLIDAKRGESMSKYEAIVWEKPWENFKKLTSFKPVA 115

[Close]

BLAST of Bhi08G000646 vs. TrEMBL
Match: tr|A0A1S3CJR9|A0A1S3CJR9_CUCME (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103501752 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.5e-37
Identity = 86/120 (71.67%), Postives = 96/120 (80.00%), Query Frame = 0


Query: 1   MNSRSASLFL---ILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQS 60
           MNSRSA  FL   +LL PLL+A  A K ++ GGW KI DV DPHV+EIA FAV +YN+QS
Sbjct: 1   MNSRSAFTFLFLTLLLTPLLAA--AWKRSVPGGWSKISDVKDPHVEEIAKFAVAQYNEQS 60

Query: 61  KGVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
           KGV IEFK V SGETQVV+G NYRLVIDAKRGES+GKY A+VWEKPWEN KKL SFKP A
Sbjct: 61  KGVAIEFKGVESGETQVVAGTNYRLVIDAKRGESIGKYEALVWEKPWENFKKLQSFKPVA 118

[Close]

BLAST of Bhi08G000646 vs. TrEMBL
Match: tr|Q9FQ13|Q9FQ13_CITPA (Cysteine proteinase inhibitor OS=Citrus paradisi OX=37656 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 8.9e-27
Identity = 69/117 (58.97%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 1   MNSRSASLFLILL--LPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSK 60
           MN R   L ++ L  +PLL+A   RKGAL+GGWK I+D  + HV EI  FAVTEYNKQSK
Sbjct: 1   MNQRFCCLIVLFLSVVPLLAA-GDRKGALVGGWKPIEDPKEKHVMEIGQFAVTEYNKQSK 60

Query: 61  GVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKP 116
              ++F+SV  GETQVVSG NYRL++  K G S  K+ A+VWEKPWE+ K LTSFKP
Sbjct: 61  S-ALKFESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWEKPWEHFKSLTSFKP 115

[Close]

BLAST of Bhi08G000646 vs. TrEMBL
Match: tr|A0A067GFH2|A0A067GFH2_CITSI (Cysteine proteinase inhibitor OS=Citrus sinensis OX=2711 GN=CISIN_1g033492mg PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 8.9e-27
Identity = 69/117 (58.97%), Postives = 84/117 (71.79%), Query Frame = 0


Query: 1   MNSRSASLFLILL--LPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSK 60
           MN R   L ++ L  +PLL+A   RKGAL+GGWK I+D  + HV EI  FAVTEYNKQSK
Sbjct: 1   MNQRFCCLIVLFLSVVPLLAA-GDRKGALVGGWKPIEDPKEKHVMEIGQFAVTEYNKQSK 60

Query: 61  GVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKP 116
              ++F+SV  GETQVVSG NYRL++  K G S  K+ A+VWEKPWE+ K LTSFKP
Sbjct: 61  S-ALKFESVEKGETQVVSGTNYRLILVVKDGPSTKKFEAVVWEKPWEHFKSLTSFKP 115

[Close]

BLAST of Bhi08G000646 vs. TrEMBL
Match: tr|A0A2I4G446|A0A2I4G446_9ROSI (Cysteine proteinase inhibitor OS=Juglans regia OX=51240 GN=LOC109004561 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-26
Identity = 65/115 (56.52%), Postives = 82/115 (71.30%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M ++   L   L LP+ SA    +G LLGGW+ I +V DPHV+EI  FAV+EYNK SK  
Sbjct: 1   MKAQFFYLLTFLFLPIFSAAAGPRGPLLGGWQPIKNVKDPHVKEIGEFAVSEYNKTSK-A 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKP 116
           +++F++VV GETQVVSG NYRLV+ A+ G     Y+A+VWEKPWEN KKLTSF P
Sbjct: 61  SLKFQNVVKGETQVVSGTNYRLVVVAEDGGVTKNYQAIVWEKPWENFKKLTSFTP 114

[Close]

BLAST of Bhi08G000646 vs. NCBI nr
Match: XP_011654999.1 (PREDICTED: cysteine proteinase inhibitor 5 [Cucumis sativus] >KGN65048.1 Cystatin-like protein [Cucumis sativus])

HSP 1 Score: 176.4 bits (446), Expect = 5.6e-41
Identity = 89/117 (76.07%), Postives = 100/117 (85.47%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M SRSAS  L+LLLPL++A  ARKG++LGGW KI D+ DPHV+EI  FAV EYNKQSKGV
Sbjct: 1   MISRSASPLLLLLLPLIAA--ARKGSMLGGWSKIPDLKDPHVEEIGKFAVAEYNKQSKGV 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
            IEFKSVVSGETQVV+G NYRL+IDAKRGES+ KY A+VWEKPWEN KKLTSFKP A
Sbjct: 61  AIEFKSVVSGETQVVAGTNYRLLIDAKRGESMSKYEAIVWEKPWENFKKLTSFKPVA 115

[Close]

BLAST of Bhi08G000646 vs. NCBI nr
Match: XP_022973098.1 (cysteine proteinase inhibitor 5-like [Cucurbita maxima])

HSP 1 Score: 168.3 bits (425), Expect = 1.5e-38
Identity = 83/117 (70.94%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M SRSASLFLILLLPLL    AR G L+GGW+KI DV DPH+QEI  FAV+EYNKQSKG 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKG- 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
            +EFK VV GE+QVVSG+NYRLVI+AK+GES+GKY+A+VWEK W++  +LTSFKP A
Sbjct: 61  ALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Bhi08G000646 vs. NCBI nr
Match: XP_022927193.1 (cysteine proteinase inhibitor 5-like [Cucurbita moschata])

HSP 1 Score: 167.9 bits (424), Expect = 2.0e-38
Identity = 82/117 (70.09%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M SRSASLFLILLLPLL    AR G L+GGW+KI DV DPH+QEI  FAV+EYNKQSKG 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKG- 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
            +EFK VV GE+QVVSG+NYRLVI+AK+GES+GKY+A++WEK W++  +LTSFKP A
Sbjct: 61  ALEFKDVVKGESQVVSGMNYRLVIEAKKGESIGKYQALIWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Bhi08G000646 vs. NCBI nr
Match: XP_023520814.1 (cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 167.5 bits (423), Expect = 2.6e-38
Identity = 83/117 (70.94%), Postives = 97/117 (82.91%), Query Frame = 0


Query: 1   MNSRSASLFLILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQSKGV 60
           M SRSASLFLILLLPLL    AR G L+GGW+KI DV DPH+QEI  FAV+EYNKQSKG 
Sbjct: 1   MKSRSASLFLILLLPLLVVATARMGRLVGGWEKIKDVKDPHIQEIGKFAVSEYNKQSKG- 60

Query: 61  TIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
            +EFK VV GE+QVVSG NYRLVI+AK+GES+GKY+A+VWEK W++  +LTSFKP A
Sbjct: 61  ALEFKDVVKGESQVVSGTNYRLVIEAKKGESIGKYQALVWEKAWQHFMELTSFKPVA 116

[Close]

BLAST of Bhi08G000646 vs. NCBI nr
Match: XP_008463660.1 (PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo])

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-37
Identity = 86/120 (71.67%), Postives = 96/120 (80.00%), Query Frame = 0


Query: 1   MNSRSASLFL---ILLLPLLSAVVARKGALLGGWKKIDDVNDPHVQEIAMFAVTEYNKQS 60
           MNSRSA  FL   +LL PLL+A  A K ++ GGW KI DV DPHV+EIA FAV +YN+QS
Sbjct: 1   MNSRSAFTFLFLTLLLTPLLAA--AWKRSVPGGWSKISDVKDPHVEEIAKFAVAQYNEQS 60

Query: 61  KGVTIEFKSVVSGETQVVSGINYRLVIDAKRGESVGKYRAMVWEKPWENSKKLTSFKPFA 118
           KGV IEFK V SGETQVV+G NYRLVIDAKRGES+GKY A+VWEKPWEN KKL SFKP A
Sbjct: 61  KGVAIEFKGVESGETQVVAGTNYRLVIDAKRGESIGKYEALVWEKPWENFKKLQSFKPVA 118

The following BLAST results are available for this feature:

BLAST of Bhi08G000646 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q6TPK4\|CYT1_ACTDE	2.8e-23	51.30	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
sp\|Q41916\|CYT5_ARATH	1.1e-22	50.00	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
sp\|Q10Q46\|CYT6_ORYSJ	1.3e-17	46.00	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
sp\|Q10J94\|CYT8_ORYSJ	3.9e-17	44.44	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
sp\|P09229\|CYT1_ORYSJ	2.8e-15	43.96	Cysteine proteinase inhibitor 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01...	[more]

BLAST of Bhi08G000646 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G47550.1	5.8e-24	50.00	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	2.5e-14	44.83	Cystatin/monellin superfamily protein	[more]
AT5G12140.1	1.6e-13	42.22	cystatin-1	[more]
AT2G40880.1	3.5e-13	33.61	cystatin A	[more]
AT5G05110.1	5.3e-09	38.46	Cystatin/monellin family protein	[more]

BLAST of Bhi08G000646 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0LTF5\|A0A0A0LTF5_CUCSA	3.7e-41	76.07	Cysteine proteinase inhibitor OS=Cucumis sativus OX=3659 GN=Csa_1G183580 PE=3 SV...	[more]
tr\|A0A1S3CJR9\|A0A1S3CJR9_CUCME	2.5e-37	71.67	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103501752 PE=3 SV=1	[more]
tr\|Q9FQ13\|Q9FQ13_CITPA	8.9e-27	58.97	Cysteine proteinase inhibitor OS=Citrus paradisi OX=37656 PE=2 SV=1	[more]
tr\|A0A067GFH2\|A0A067GFH2_CITSI	8.9e-27	58.97	Cysteine proteinase inhibitor OS=Citrus sinensis OX=2711 GN=CISIN_1g033492mg PE=...	[more]
tr\|A0A2I4G446\|A0A2I4G446_9ROSI	1.2e-26	56.52	Cysteine proteinase inhibitor OS=Juglans regia OX=51240 GN=LOC109004561 PE=3 SV=...	[more]

BLAST of Bhi08G000646 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654999.1	5.6e-41	76.07	PREDICTED: cysteine proteinase inhibitor 5 [Cucumis sativus] >KGN65048.1 Cystati...	[more]
XP_022973098.1	1.5e-38	70.94	cysteine proteinase inhibitor 5-like [Cucurbita maxima]	[more]
XP_022927193.1	2.0e-38	70.09	cysteine proteinase inhibitor 5-like [Cucurbita moschata]	[more]
XP_023520814.1	2.6e-38	70.94	cysteine proteinase inhibitor 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_008463660.1	3.8e-37	71.67	PREDICTED: cysteine proteinase inhibitor 5 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004869	cysteine-type endopeptidase inhibitor activity

Vocabulary: **INTERPRO**
Term	Definition
IPR018073	Prot_inh_cystat_CS
IPR027214	Cystatin
IPR000010	Cystatin_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0034605	cellular response to heat
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0010030	positive regulation of seed germination
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0008150	biological_process
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi08M000646	Bhi08M000646	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 26..116 e-value: 2.3E-23 score: 93.6
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 34..115 e-value: 2.1E-32 score: 111.2
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 29..115 e-value: 1.30916E-20 score: 79.8256
None	No IPR available	GENE3D	G3DSA:3.10.450.10		coord: 21..117 e-value: 3.2E-36 score: 125.4
None	No IPR available	PANTHER	PTHR11413:SF65	CYSTEINE PROTEINASE INHIBITOR 5	coord: 8..115
None	No IPR available	PRODOM	PD001231		coord: 44..115 e-value: 1.0E-19 score: 232.0
None	No IPR available	SUPERFAMILY	SSF54403	Cystatin/monellin	coord: 29..116
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 8..115
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 72..85

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi08G000646	ClCG01G010410	Watermelon (Charleston Gray)	wcgwgoB238
Bhi08G000646	CmaCh13G003710	Cucurbita maxima (Rimu)	cmawgoB0275
Bhi08G000646	Lsi02G011120	Bottle gourd (USVL1VR-Ls)	lsiwgoB250
Bhi08G000646	Cp4.1LG09g06390	Cucurbita pepo (Zucchini)	cpewgoB0042
Bhi08G000646	Carg25618	Silver-seed gourd	carwgoB0057
Bhi08G000646	Cla009017	Watermelon (97103) v1	wgowmB505
Bhi08G000646	Cla97C01G010280	Watermelon (97103) v2	wgowmbB456

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Bhi08G000646	Cucumber (Gy14) v1	cgywgoB370
Bhi08G000646	Cucumber (Gy14) v2	cgybwgoB032
Bhi08G000646	Cucumber (Gy14) v2	cgybwgoB042
Bhi08G000646	Cucumber (Gy14) v2	cgybwgoB607
Bhi08G000646	Cucurbita maxima (Rimu)	cmawgoB0260
Bhi08G000646	Cucurbita maxima (Rimu)	cmawgoB0535
Bhi08G000646	Cucurbita moschata (Rifu)	cmowgoB0255
Bhi08G000646	Cucurbita moschata (Rifu)	cmowgoB0264
Bhi08G000646	Wild cucumber (PI 183967)	cpiwgoB036
Bhi08G000646	Cucumber (Chinese Long) v3	cucwgoB039
Bhi08G000646	Cucumber (Chinese Long) v2	cuwgoB036
Bhi08G000646	Cucumber (Chinese Long) v2	cuwgoB047
Bhi08G000646	Bottle gourd (USVL1VR-Ls)	lsiwgoB257
Bhi08G000646	Melon (DHL92) v3.6.1	medwgoB182
Bhi08G000646	Melon (DHL92) v3.5.1	mewgoB167
Bhi08G000646	Watermelon (Charleston Gray)	wcgwgoB241
Bhi08G000646	Watermelon (97103) v2	wgowmbB460
Bhi08G000646	Watermelon (97103) v1	wgowmB511
Bhi08G000646	Silver-seed gourd	carwgoB1216