Bhi07G000043 (gene) Wax gourd

NameBhi07G000043
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionCalcium-binding EF-hand
Locationchr7 : 2667084 .. 2667569 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATGAAGTAAGTAAGATGATCAACGAATATGAAAACAATGGTGATGGTATTCAAGTAAAAGCTTTCAATGATGTTGCTCGAGGTTCAACTCAAATCAACTATCAAAGAACGGTATTGAAAATTCGAAGACCTAACGATGATGTTGTACACACAGTTGGTTCAAATGGTCATATAAAACCAACTAAGCAAATAGGAGGAAAGGGTAAAAAATCTACCACAAGGGTTCTTGACACAAAGTCATCTAATATGCAATTTAGTCGAGAGAAGATAAGAGAAATTTTCAAGTGTCATGATAGCAACAATGATGGTTTCCTCAACAAAAGGGAGTTAACAAAAGCTTTTGCATTTCTAGGCTCAATGTTTCCATTCAACAAAGCTTGCTATGCAATGGTGTACGCCGATGCTAATGAAGACGGTCTTATCAATGATGCTAAGCTTGACGAACTCATAGATTATGCAAGTAAATTTATCCAAATGAAATGA

mRNA sequence

ATGCATGAAGTAAGTAAGATGATCAACGAATATGAAAACAATGGTGATGGTATTCAAGTAAAAGCTTTCAATGATGTTGCTCGAGGTTCAACTCAAATCAACTATCAAAGAACGGTATTGAAAATTCGAAGACCTAACGATGATGTTGTACACACAGTTGGTTCAAATGGTCATATAAAACCAACTAAGCAAATAGGAGGAAAGGGTAAAAAATCTACCACAAGGGTTCTTGACACAAAGTCATCTAATATGCAATTTAGTCGAGAGAAGATAAGAGAAATTTTCAAGTGTCATGATAGCAACAATGATGGTTTCCTCAACAAAAGGGAGTTAACAAAAGCTTTTGCATTTCTAGGCTCAATGTTTCCATTCAACAAAGCTTGCTATGCAATGGTGTACGCCGATGCTAATGAAGACGGTCTTATCAATGATGCTAAGCTTGACGAACTCATAGATTATGCAAGTAAATTTATCCAAATGAAATGA

Coding sequence (CDS)

ATGCATGAAGTAAGTAAGATGATCAACGAATATGAAAACAATGGTGATGGTATTCAAGTAAAAGCTTTCAATGATGTTGCTCGAGGTTCAACTCAAATCAACTATCAAAGAACGGTATTGAAAATTCGAAGACCTAACGATGATGTTGTACACACAGTTGGTTCAAATGGTCATATAAAACCAACTAAGCAAATAGGAGGAAAGGGTAAAAAATCTACCACAAGGGTTCTTGACACAAAGTCATCTAATATGCAATTTAGTCGAGAGAAGATAAGAGAAATTTTCAAGTGTCATGATAGCAACAATGATGGTTTCCTCAACAAAAGGGAGTTAACAAAAGCTTTTGCATTTCTAGGCTCAATGTTTCCATTCAACAAAGCTTGCTATGCAATGGTGTACGCCGATGCTAATGAAGACGGTCTTATCAATGATGCTAAGCTTGACGAACTCATAGATTATGCAAGTAAATTTATCCAAATGAAATGA

Protein sequence

MHEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTVLKIRRPNDDVVHTVGSNGHIKPTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK
BLAST of Bhi07G000043 vs. TrEMBL
Match: tr|A0A0A0KMQ1|A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.3e-54
Identity = 109/161 (67.70%), Postives = 133/161 (82.61%), Query Frame = 0

Query: 1   MHEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTVLKIRRPNDDVVHTVGSNGHIK 60
           M+E S +I +YE NGD ++VKAF+DV R STQI  + +VLKI +P+D V H + S  HI+
Sbjct: 12  MYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGVAHKLRSEAHIQ 71

Query: 61  PTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGS 120
           PT++ GGKGKKS TRVLD KSSNM  SRE+I+EIF+ HD+NNDGFLN+ ELTKAFAFLGS
Sbjct: 72  PTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGS 131

Query: 121 MFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           MFPF KACY MVYADANEDGLI++A+LD+LIDYASK I+ K
Sbjct: 132 MFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

BLAST of Bhi07G000043 vs. TrEMBL
Match: tr|A0A0A0KLF3|A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.8e-44
Identity = 94/161 (58.39%), Postives = 123/161 (76.40%), Query Frame = 0

Query: 2   HEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTV-LKIRRPNDDVVHTVGSNGHIK 61
           +E SK++ +YE NG+G+ + AFND ARGSTQIN  R +  K  +PNDDV H + SN  +K
Sbjct: 40  YEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLK 99

Query: 62  PTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGS 121
              QI GK +    RV DTK+SNMQFSRE+I+EIF+ +DS+ DGFLN RE+TKAFA LGS
Sbjct: 100 SNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGS 159

Query: 122 MFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           +FPFNKA + MVYAD N+DGLI + +LD+LI+YA+KF++ K
Sbjct: 160 IFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

BLAST of Bhi07G000043 vs. TrEMBL
Match: tr|A0A0A0KIY4|A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.1e-38
Identity = 85/164 (51.83%), Postives = 123/164 (75.00%), Query Frame = 0

Query: 2   HEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQR---TVLKIRRPNDDVVHTVGSNGH 61
           +E SK++ ++ENN +G+ +KAFN +   ST+IN ++   T + ++  N  V H + SN +
Sbjct: 13  YEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSNAY 72

Query: 62  IKPTKQIG-GKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAF 121
           + P+KQ+G GK + +TT VLD K+SNMQFSRE+I+EIF+ HDS+NDGFLN RE+TKAFAF
Sbjct: 73  MIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAF 132

Query: 122 LGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           LG++ P  KA + MVYADAN+DGLI+  +L++L+DYA KF   +
Sbjct: 133 LGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

BLAST of Bhi07G000043 vs. TrEMBL
Match: tr|A0A0A0LRC8|A0A0A0LRC8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-31
Identity = 80/166 (48.19%), Postives = 114/166 (68.67%), Query Frame = 0

Query: 2   HEVSKMINEYENNGDGI----QVKAFNDVARGSTQINYQRTVLK--IRRPNDDVVHTVGS 61
           +E +++I +Y+ NGDG+    ++++F   ARGSTQ+N  R V    + + N+++   V S
Sbjct: 12  NEANELIKKYDRNGDGVLTKEELQSFLRDARGSTQLNNIRNVSSSTVPKTNENLAQKVSS 71

Query: 62  NGHIKPTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAF 121
           N  I P KQ+  K + S      +K SN+  SRE+I+EIFK HDSN DGFL+ RELTKAF
Sbjct: 72  NTCITPKKQLLDKAQGS------SKQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAF 131

Query: 122 AFLGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           + +GS+ PF KA YAM YAD +EDGL+++A+LD+LIDYA K I+ K
Sbjct: 132 SSMGSIVPFCKARYAMAYADVDEDGLVSEAELDKLIDYAHKIIKKK 171

BLAST of Bhi07G000043 vs. TrEMBL
Match: tr|A0A1S3BDW2|A0A1S3BDW2_CUCME (probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 PE=4 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.1e-26
Identity = 75/161 (46.58%), Postives = 101/161 (62.73%), Query Frame = 0

Query: 3   EVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTVLK--IRRPNDDVVHTVGSNGHIK 62
           E S++I +Y+ NGDG+  K          ++   R V    I + N+++   V SN  IK
Sbjct: 13  EASELIKKYDRNGDGVLTKEELQSFLHDARLKNIRFVSSSTIPKTNENLDQKVSSNTRIK 72

Query: 63  PTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGS 122
           P KQ+  K + S       + SN+  SRE+I+EIFK HDSN DGFL+ RELTKAF  LGS
Sbjct: 73  PKKQLLDKAQDS------LRQSNVPLSREQIKEIFKYHDSNKDGFLSIRELTKAFGSLGS 132

Query: 123 MFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           + PF KA +AM YAD +EDGLI++A+L +LIDYA K I+ K
Sbjct: 133 IVPFAKARHAMAYADVDEDGLISEAELHKLIDYAEKIIKKK 167

BLAST of Bhi07G000043 vs. NCBI nr
Match: KGN49687.1 (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 219.5 bits (558), Expect = 8.0e-54
Identity = 109/161 (67.70%), Postives = 133/161 (82.61%), Query Frame = 0

Query: 1   MHEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTVLKIRRPNDDVVHTVGSNGHIK 60
           M+E S +I +YE NGD ++VKAF+DV R STQI  + +VLKI +P+D V H + S  HI+
Sbjct: 12  MYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGVAHKLRSEAHIQ 71

Query: 61  PTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGS 120
           PT++ GGKGKKS TRVLD KSSNM  SRE+I+EIF+ HD+NNDGFLN+ ELTKAFAFLGS
Sbjct: 72  PTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGS 131

Query: 121 MFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           MFPF KACY MVYADANEDGLI++A+LD+LIDYASK I+ K
Sbjct: 132 MFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

BLAST of Bhi07G000043 vs. NCBI nr
Match: KGN49689.1 (hypothetical protein Csa_5G067670 [Cucumis sativus])

HSP 1 Score: 186.8 bits (473), Expect = 5.7e-44
Identity = 94/161 (58.39%), Postives = 123/161 (76.40%), Query Frame = 0

Query: 2   HEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQRTV-LKIRRPNDDVVHTVGSNGHIK 61
           +E SK++ +YE NG+G+ + AFND ARGSTQIN  R +  K  +PNDDV H + SN  +K
Sbjct: 40  YEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDDVAHKLSSNAQLK 99

Query: 62  PTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAFLGS 121
              QI GK +    RV DTK+SNMQFSRE+I+EIF+ +DS+ DGFLN RE+TKAFA LGS
Sbjct: 100 SNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGS 159

Query: 122 MFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           +FPFNKA + MVYAD N+DGLI + +LD+LI+YA+KF++ K
Sbjct: 160 IFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

BLAST of Bhi07G000043 vs. NCBI nr
Match: XP_023538534.1 (putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 171.0 bits (432), Expect = 3.3e-39
Identity = 89/164 (54.27%), Postives = 121/164 (73.78%), Query Frame = 0

Query: 3   EVSKMINEYENNGDGI----QVKAFNDVARGSTQI-NYQRTVLKIRRPNDDVVHTVGSNG 62
           E ++++ +Y+ NGDG+    +++ F   ARGSTQ+ N Q  V KI + +++VVH   SN 
Sbjct: 13  EANRLMKKYDTNGDGMLTKEELQVFLHKARGSTQLNNNQGFVSKIPKASNNVVHKHSSNS 72

Query: 63  HIKPTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAF 122
           H+K  KQ+  KGKKS+T+ LD + S +Q SRE+++EIFK HDSN DGFL+ +E+TKAF F
Sbjct: 73  HMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTKAFGF 132

Query: 123 LGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           LGS+ PFNKA YAM YAD N DGLI++ +LD+LIDYASKFI  K
Sbjct: 133 LGSILPFNKAHYAMTYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Bhi07G000043 vs. NCBI nr
Match: XP_022951134.1 (uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata])

HSP 1 Score: 169.9 bits (429), Expect = 7.3e-39
Identity = 88/164 (53.66%), Postives = 121/164 (73.78%), Query Frame = 0

Query: 3   EVSKMINEYENNGDGI----QVKAFNDVARGSTQI-NYQRTVLKIRRPNDDVVHTVGSNG 62
           E ++++ +Y+ NGDG+    +++ F   ARGSTQ+ N Q  V KI + +++V H   SN 
Sbjct: 13  EANRLMKKYDTNGDGMLTKEELQVFLHKARGSTQLNNNQGFVSKIPKASNNVAHKHSSNS 72

Query: 63  HIKPTKQIGGKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAF 122
           H+K  KQ+  KGKKS+T+ LD + S +Q SRE+++EIFK HDSN DGFL+ +E+TKAF+F
Sbjct: 73  HMKHKKQVQDKGKKSSTKFLDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTKAFSF 132

Query: 123 LGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           LGS+ PFNKA YAM YAD N DGLI++ +LD+LIDYASKFI  K
Sbjct: 133 LGSILPFNKAHYAMAYADTNHDGLISEFELDKLIDYASKFINKK 176

BLAST of Bhi07G000043 vs. NCBI nr
Match: KGN49690.1 (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 167.2 bits (422), Expect = 4.7e-38
Identity = 85/164 (51.83%), Postives = 123/164 (75.00%), Query Frame = 0

Query: 2   HEVSKMINEYENNGDGIQVKAFNDVARGSTQINYQR---TVLKIRRPNDDVVHTVGSNGH 61
           +E SK++ ++ENN +G+ +KAFN +   ST+IN ++   T + ++  N  V H + SN +
Sbjct: 13  YEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSNHPVAHYLTSNAY 72

Query: 62  IKPTKQIG-GKGKKSTTRVLDTKSSNMQFSREKIREIFKCHDSNNDGFLNKRELTKAFAF 121
           + P+KQ+G GK + +TT VLD K+SNMQFSRE+I+EIF+ HDS+NDGFLN RE+TKAFAF
Sbjct: 73  MIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAF 132

Query: 122 LGSMFPFNKACYAMVYADANEDGLINDAKLDELIDYASKFIQMK 162
           LG++ P  KA + MVYADAN+DGLI+  +L++L+DYA KF   +
Sbjct: 133 LGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
tr|A0A0A0KMQ1|A0A0A0KMQ1_CUCSA5.3e-5467.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1[more]
tr|A0A0A0KLF3|A0A0A0KLF3_CUCSA3.8e-4458.39Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1[more]
tr|A0A0A0KIY4|A0A0A0KIY4_CUCSA3.1e-3851.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1[more]
tr|A0A0A0LRC8|A0A0A0LRC8_CUCSA6.3e-3148.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359950 PE=4 SV=1[more]
tr|A0A1S3BDW2|A0A1S3BDW2_CUCME2.1e-2646.58probable calcium-binding protein CML11 OS=Cucumis melo OX=3656 GN=LOC103488544 P... [more]
Match NameE-valueIdentityDescription
KGN49687.18.0e-5467.70hypothetical protein Csa_5G067410 [Cucumis sativus][more]
KGN49689.15.7e-4458.39hypothetical protein Csa_5G067670 [Cucumis sativus][more]
XP_023538534.13.3e-3954.27putative calmodulin-like protein 6 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022951134.17.3e-3953.66uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata][more]
KGN49690.14.7e-3851.83hypothetical protein Csa_5G068170 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
Vocabulary: INTERPRO
TermDefinition
IPR011992EF-hand-dom_pair
IPR002048EF_hand_dom
IPR018247EF_Hand_1_Ca_BS
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi07M000043Bhi07M000043mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 52..153
e-value: 1.3E-10
score: 43.2
NoneNo IPR availablePANTHERPTHR44455:SF5SUBFAMILY NOT NAMEDcoord: 81..158
NoneNo IPR availablePANTHERPTHR44455FAMILY NOT NAMEDcoord: 81..158
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 99..111
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 86..121
score: 12.868
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 1..33
score: 5.28
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 83..143