Bhi06G001328 (gene) Wax gourd

NameBhi06G001328
Typegene
OrganismBenincasa hispida (Wax gourd)
DescriptionPseudouridine synthase
Locationchr6 : 44731033 .. 44733998 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTTTTCTAATTTACATAAATGGCCCTCCTCATCTCCCTTCAGCCCACCTTTTCAACTCCGCACAAAACTTTCACCGAATTCCCAAAACCCTTCTGTTCCGGTAGATTCAAATCCATCAGCAAGAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCTGTTCCCTTATCTCCTCCACTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGATGAGTGGCTCATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCAGTGATTCTGCTATGGCTGGTTTGTGGATCTTTACCGTGAAACCATTTTTTTAGCTGATGAATTCGAATTTTGACATGAACTTGGCAAGATGAATTCAATTTTACCCTGCATTTTGAATTTTCTATTCGAATAGAAATGGTGGAATTGCAACTTTTAACTTGCTCTCGTTTGAAGATTTGTGAAAAGGTGCAATCATGTGCTTCCATGGATTACAACAATGTAGACAACGCTGCAAAGGAAGAAGATATTAGGGCTGAATTTTTGTTAAAATTGACGAGTTTTGAAAGATTTGTTCAATATAGATCGCGTGCATTGGTTTTCTAGATCATACTTTTATGCATAGATATTTAACTTGGTTTTTGTTGACATTAGCTGAAATTAGTGGGTAAATGTGATGGAAGTTCAACAGAAGTTTAGAACTGTAATGCTTATCTACGTGTAATGTCCATCTTGATGAAATGTTGAAGTTTGGAGTAATAATTGACTCACCTTGCTAAGTTCTGGGTAGGATTTGATCTTTCATCAAATTTGTTTACAAGGAATGATTTCTGGAATTTATTTTTCATCGGTTTAAGCTGTTTAAAAATGCAATTTCTCAAGTTCTTCTTCTTCTTCTTCTTTTTTTTTTTTAGAGACCCCGGTGTGAGGTTTGGCCTTGTATTTTGGTTCAACATGTCTTTGCGAGCGTCCGTAACTAAATTTTTGCTAGACTTGAGGCCTTTTTTTTCTAGGTTGCCTCACTTTTGTTGGCTTTCTCTTTTTATATTCTCAAATGAACGTTCGGTTATTCATTAAGAAAAAATTTCTGTCCTTTCCTTTTTTTTTTTTTTTTTTTTTAATTGAAACTCATTGTCTAATGGTTCTTCAAGAGAGACAAAAATCCATTACTTGAGCATTGTTTTCATAGAGGGCTCTAGGTTATCTCATCAAATTATATGATATTGATGTTTTCTGGGGTGCTCTGTGATATTGATATCATGATAGGTGAGGCTGCTCTTGTAGTTTGATATGAATACATTTACTAAAGAGGCGAGAGCAAGTAATTGATTTCTGATTCTTTCCCTACATTTATAGCAATGTTGCTGATTGTTAAACAGTTGATATAAATGGTTTAGATTTATCCTGATTGTTGAAGAGAGAGGTGCTTAAATGTGTCTGGAGTGTTTTAACGTTTGTCTAAAACAATGCCATGTGATCACATCTTTTGCTTATTCTATCATTATTGATTTCTCGGGAGTGTTCTCATCATATTCTTCATATGCAGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAGTCATATATCGCCTTCTGCGTTGGCTCTGCTCCAAAGTGGAAAAAAATCAACATCAAATCTGGTCATGGTAGGTCAAAATTTGGGGTATGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCTGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTATAAATGGACAAAACGCCATGGAAGAATTTCAAAAATTTGGAAAAGATGAAGAAACTGTCGTTGTCCAAACAAAATCTCTAGTAGACATTGATGGTCAAAAGGACGAGATATTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAACGGAAAAATTTATGATTGCCATGAGCTTCATGCCGAAAGCTTGTATTTTGTGCACCCTATTACAGGTATTCCTCTCAAGTTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATGGCAGCCCCAACAACAAGAAGTGAATTCTCCATAGTCCTTCAAAGCTATGCCTTAAGAAAATCATTGGCACAATTAAGTGAGAACCAAACATATCTCTTACCATACAAAGAGCTTCGGTATCGAAGAATGGCGTTGAAGCAAAATGGAGCAAATCTTCAATCAAATTCGTAAAGTGTGACTGCAGGCAGTTTTCACACCATCAGCTTCCTGTCTGCAATTTCTCATCTATTGAACGAGCTCTTGTTAGCACAACAGTGTCTACGAAAATATCAGCAGTCGTAAACCGATCAAACCATCTGGGTTTTTCCTGAAAATTTGAGAGGACGGGATGCTCGCAATGGATTCTGGAAATGACAGAAGAATCATGCGTGTCTAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGATCTTCTGCCGCAGTTTTATCGATAAAATTAATGTACAGAGTCGAAGCAGCCAAATTATATGGTTAAACATGTTGATTTCTGTTGAGGCTCAATTGTTATTTGACTCCCCTTCCCTCCATTTGTGCAACTTTGTGTTAAAAATATGACGAACAATTTTGTTATGATGCTTACGCGTCTATAAAATATAAATAGAAGAATGAGAAAACATGTTGAGTTTTTGTCTTCTCTTATTATTTTTTAAAGGAAAATAC

mRNA sequence

AATTTTTCTAATTTACATAAATGGCCCTCCTCATCTCCCTTCAGCCCACCTTTTCAACTCCGCACAAAACTTTCACCGAATTCCCAAAACCCTTCTGTTCCGGTAGATTCAAATCCATCAGCAAGAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCTGTTCCCTTATCTCCTCCACTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGATGAGTGGCTCATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCAGTGATTCTGCTATGGCTGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAGTCATATATCGCCTTCTGCGTTGGCTCTGCTCCAAAGTGGAAAAAAATCAACATCAAATCTGGTCATGGTAGGTCAAAATTTGGGGTATGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCTGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTATAAATGGACAAAACGCCATGGAAGAATTTCAAAAATTTGGAAAAGATGAAGAAACTGTCGTTGTCCAAACAAAATCTCTAGTAGACATTGATGGTCAAAAGGACGAGATATTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAACGGAAAAATTTATGATTGCCATGAGCTTCATGCCGAAAGCTTGTATTTTGTGCACCCTATTACAGGTATTCCTCTCAAGTTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATGGCAGCCCCAACAACAAGAAGTGAATTCTCCATAGTCCTTCAAAGCTATGCCTTAAGAAAATCATTGGCACAATTAAGTGAGAACCAAACATATCTCTTACCATACAAAGAGCTTCGGTATCGAAGAATGGCGTTGAAGCAAAATGGAGCAAATCTTCAATCAAATTCGTAAAGTGTGACTGCAGGCAGTTTTCACACCATCAGCTTCCTGTCTGCAATTTCTCATCTATTGAACGAGCTCTTGTTAGCACAACAGTGTCTACGAAAATATCAGCAGTCGTAAACCGATCAAACCATCTGGGTTTTTCCTGAAAATTTGAGAGGACGGGATGCTCGCAATGGATTCTGGAAATGACAGAAGAATCATGCGTGTCTAAGGCTGATAAATTTGTGCAAAGCCCTTTGGAAATGGATCTTCTGCCGCAGTTTTATCGATAAAATTAATGTACAGAGTCGAAGCAGCCAAATTATATGGTTAAACATGTTGATTTCTGTTGAGGCTCAATTGTTATTTGACTCCCCTTCCCTCCATTTGTGCAACTTTGTGTTAAAAATATGACGAACAATTTTGTTATGATGCTTACGCGTCTATAAAATATAAATAGAAGAATGAGAAAACATGTTGAGTTTTTGTCTTCTCTTATTATTTTTTAAAGGAAAATAC

Coding sequence (CDS)

ATGGCCCTCCTCATCTCCCTTCAGCCCACCTTTTCAACTCCGCACAAAACTTTCACCGAATTCCCAAAACCCTTCTGTTCCGGTAGATTCAAATCCATCAGCAAGAGCATGGCGGACTCATCGGAACCGGTGACCGGAAACTGCCCATCAACCAGAGAGAATTACCCTGTTCCCTTATCTCCTCCACTGCCGGCCATATCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTCCTCTAAATCCAGCCTCTTTGCTTTATCGGCAAACGATGTTATATACGAGGATGAGTGGCTCATTGCCGTCAATAAGCCTCAAGGAATCTACTGTGAAAGCGTTTTGGCCGCTGTTCCTCGTCTTCTCAGTGATTCTGCTATGGCTGGGATCAAAACCAGTCTGCCAGAGCTTCATCTTGCTAACCGCCTTGATCGTGACACAAGTGGGGTTATGGTTATAACTAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACACAGTTTCAAAGTCATATATCGCCTTCTGCGTTGGCTCTGCTCCAAAGTGGAAAAAAATCAACATCAAATCTGGTCATGGTAGGTCAAAATTTGGGGTATGGCGTGTCTATGCTGCAGCTGATGTGGGTCGTTCCTTACCTGGCGGTTCTGTAGTCAGAGACATGGAAACATATTTTGAAGTACTATCTATAAATGGACAAAACGCCATGGAAGAATTTCAAAAATTTGGAAAAGATGAAGAAACTGTCGTTGTCCAAACAAAATCTCTAGTAGACATTGATGGTCAAAAGGACGAGATATTGATAAGAGCACGACCTCGCAGTGGAAGAACTCATCAAATCCGTCTGCACTGCCAATATCTTGGAATTCCTATAAGAGGGGATGTGAAATATGAGGGTGTTACAGAGTGGAACGGAAAAATTTATGATTGCCATGAGCTTCATGCCGAAAGCTTGTATTTTGTGCACCCTATTACAGGTATTCCTCTCAAGTTACAAGCTCCTCTACCATCATGGGCCAGCCAAGCATGGCAGCCCCAACAACAAGAAGTGAATTCTCCATAG

Protein sequence

MALLISLQPTFSTPHKTFTEFPKPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP
BLAST of Bhi06G001328 vs. Swiss-Prot
Match: sp|Q2QNM3|PUS1_ORYSJ (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 4.2e-99
Identity = 182/295 (61.69%), Postives = 223/295 (75.59%), Query Frame = 0

Query: 55  YPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVL 114
           YPVP+SPP PA SK++EL RAM AS++S+  A S+  V++EDEWL  V+KP G+YC+++L
Sbjct: 51  YPVPVSPPYPAASKDVELRRAMTASARSA--AYSSAPVVFEDEWLAVVDKPAGVYCDALL 110

Query: 115 AAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKS 174
           +A+P     +A  G + + P LHLANRLDRDTSG+MVITK +KVA KLVKAFT+H V K+
Sbjct: 111 SALP---CSAATLGDEATKPNLHLANRLDRDTSGLMVITKCNKVAGKLVKAFTEHKVKKT 170

Query: 175 YIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSIN 234
           Y+A C+G  P W+KI I SGHGRSK G WRVYA +DVGRSLPGGSVVRDM T FEVL IN
Sbjct: 171 YLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVRDMSTRFEVLGIN 230

Query: 235 GQNAMEEFQKFGKDE-ETVVVQTKSLVDIDGQKDE----ILIRARPRSGRTHQIRLHCQY 294
           G+    E   F  DE E++ VQ K+  D+    DE    IL+RA P+SGRTHQIRLHCQY
Sbjct: 231 GKGQFREPSNFEVDETESITVQEKA-ADLTSDGDEKNSIILVRAYPQSGRTHQIRLHCQY 290

Query: 295 LGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQ 345
           LG PIRGDVKY GV EWNG  YD H LHAESL FVHP+TG+P+  ++PLPSWA++
Sbjct: 291 LGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFRSPLPSWANE 339

BLAST of Bhi06G001328 vs. Swiss-Prot
Match: sp|Q7XA65|PUS1_ARATH (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.7e-95
Identity = 173/300 (57.67%), Postives = 218/300 (72.67%), Query Frame = 0

Query: 54  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 113
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 114 LAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 173
           L + P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 174 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSI 233
           SYIA C+GS+P W+++ + SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+S+
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 234 NGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKD----------EILIRARPRSGRTHQI 293
           N            K+E   +     ++  +G+++              RA PRSGRTHQI
Sbjct: 219 NSV----------KNESCELEDVNHVIVAEGERELSCGXXXXXXXXXXRAFPRSGRTHQI 278

Query: 294 RLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWAS 344
           RLHCQYLGIPIRGDVKY GV EWNG+ ++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 RLHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of Bhi06G001328 vs. Swiss-Prot
Match: sp|Q68XB2|RLUC_RICTY (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=rluC PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 9.6e-19
Identity = 81/250 (32.40%), Postives = 117/250 (46.80%), Query Frame = 0

Query: 92  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMV 151
           +IYED+ LIA+NKP  +  +    +   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 152 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADV 211
           I K++  + KL  AF +  V K Y A   G   K   I +KS  G+SK            
Sbjct: 151 IAKNYLSSVKLHDAFKEKLVIKKYFAITYGRPVKNVGI-VKSNIGKSK------------ 210

Query: 212 GRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEILI 271
           GR              F++  I+  N        GK   T     KSL       +  LI
Sbjct: 211 GR-------------MFKITDIDSDN--------GKLAITYYKLLKSL-----NNNLFLI 270

Query: 272 RARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVTEWNGKIYDCHELHAESLYFVHPITG 331
              P +GR HQ+RLH Q LG PI GD KY  + V  ++  ++    LHA ++Y    I G
Sbjct: 271 EFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKYMF----LHANNIYLSESIVG 295

Query: 332 IPLKLQAPLP 340
             +KL+A LP
Sbjct: 331 KEIKLEAKLP 295

BLAST of Bhi06G001328 vs. Swiss-Prot
Match: sp|Q9ZDR7|RLUC_RICPR (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=rluC PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.4e-17
Identity = 75/250 (30.00%), Postives = 116/250 (46.40%), Query Frame = 0

Query: 92  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMV 151
           +IYED+ LIA+NKP  +  +    +   L  DSA+  +     +  L +RLD++TSG+++
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFKLVHRLDKETSGLLL 150

Query: 152 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADV 211
           I K++  + KL  AF +  V K Y A   G  P      +KS  G+SK            
Sbjct: 151 IAKNYLSSVKLHNAFKEKLVIKKYFAITYG-RPVKNVGTVKSNIGKSK------------ 210

Query: 212 GRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEILI 271
                     R +     + S +G+ A+  ++             KSL       +  LI
Sbjct: 211 ----------RRLSKIANIDSDDGKLAITYYKLL-----------KSL-----NNNLFLI 270

Query: 272 RARPRSGRTHQIRLHCQYLGIPIRGDVKY--EGVTEWNGKIYDCHELHAESLYFVHPITG 331
              P +GR HQ+RLH Q LG PI GD KY  + +  ++  ++    LHA ++Y    I G
Sbjct: 271 EFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEIMPYSKYMF----LHANNIYLSETIVG 295

Query: 332 IPLKLQAPLP 340
             +KL+A LP
Sbjct: 331 KEIKLEAKLP 295

BLAST of Bhi06G001328 vs. Swiss-Prot
Match: sp|Q1RJX7|RLUC_RICBR (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=rluC PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.2e-16
Identity = 85/314 (27.07%), Postives = 138/314 (43.95%), Query Frame = 0

Query: 34  SKSMADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVI 93
           SK    S   V G+     + + +P++ P   +  + E+  A           ++   +I
Sbjct: 42  SKKAEASLRVVEGDEIFIHDKFNLPIAQPTKLVFTDAEITLAK---------KITTEYLI 101

Query: 94  YEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVIT 153
           YED+ +IA+NKP G+  +    +   L  DSA+  + +   +  L +RLD++TSG+++I 
Sbjct: 102 YEDDNIIAINKPAGLATQG--GSKINLSVDSALKYLNSQGADFKLVHRLDKETSGLLLIA 161

Query: 154 KSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWK---KINIKSGHGRSKFGVWRVYAAAD 213
           K++  + KL  AF +  V K+Y+A   G   K +   K NI+   G              
Sbjct: 162 KNYVSSVKLHDAFKEKLVEKTYLAVTYGKPIKDQGEVKTNIEKSKG-------------- 221

Query: 214 VGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEIL 273
              S+P            ++  IN  N        GK   T     KSL +     +  L
Sbjct: 222 ---SIP------------KITDINDNN--------GKLAITYYKLLKSLTN-----NLFL 281

Query: 274 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHE---LHAESLYFVHPI 333
           +   P +GR HQ+RLH + LG PI GD KY      N K+    +   LHA ++     +
Sbjct: 282 VEFIPITGRMHQLRLHAKLLGCPIFGDNKY-----GNDKLMPYSKYMFLHANNIVLSKKV 297

Query: 334 TGIPLKLQAPLPSW 342
            G  + L+A LPS+
Sbjct: 342 FGKEVNLEAKLPSY 297

BLAST of Bhi06G001328 vs. TAIR10
Match: AT1G56345.1 (Pseudouridine synthase family protein)

HSP 1 Score: 350.9 bits (899), Expect = 9.2e-97
Identity = 173/300 (57.67%), Postives = 218/300 (72.67%), Query Frame = 0

Query: 54  NYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESV 113
           NYP P+S P P ISK++EL RAM ASSKSSLF L+ +D++YEDE+L+AVNKP+G+YCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 114 LAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 173
           L + P+++ DS+         E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+H + K
Sbjct: 99  LRSAPQIVLDSS--------SEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRK 158

Query: 174 SYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSI 233
           SYIA C+GS+P W+++ + SGHGRSK G WRVYAA DVGR LPGGS VRDMET FEV+S+
Sbjct: 159 SYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSV 218

Query: 234 NGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKD----------EILIRARPRSGRTHQI 293
           N            K+E   +     ++  +G+++              RA PRSGRTHQI
Sbjct: 219 NSV----------KNESCELEDVNHVIVAEGERELSCGXXXXXXXXXXRAFPRSGRTHQI 278

Query: 294 RLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWAS 344
           RLHCQYLGIPIRGDVKY GV EWNG+ ++ HELHAE L   HP+TG  + ++APLP WA+
Sbjct: 279 RLHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWAA 320

BLAST of Bhi06G001328 vs. TAIR10
Match: AT1G76050.2 (Pseudouridine synthase family protein)

HSP 1 Score: 69.7 bits (169), Expect = 4.1e-12
Identity = 73/307 (23.78%), Postives = 113/307 (36.81%), Query Frame = 0

Query: 91  DVIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGI--KTSLPELHLAN-------- 150
           D++YED+ ++ VNKP       V+   P   + + + GI    SLP +  +N        
Sbjct: 149 DIVYEDKHVLVVNKP----AHMVVHPAPGNPTGTLVNGILHHCSLPCVDYSNXXXXXXXX 208

Query: 151 ---------------------------RLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 210
                                      RLD+ T+G++V+ K     + L + F  HT+ +
Sbjct: 209 XXXXXXXXXXXXXXXXYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIER 268

Query: 211 SYIAFCVG-SAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS 270
            Y++   G  +P   +I I  G   S     R+  AA     +PGG              
Sbjct: 269 VYVSLTTGVPSPPQGRIEIPIGRDSSN----RIRMAA-----IPGG-------------- 328

Query: 271 INGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGI 330
           + G  A     ++             +++        L+  R  +GRTHQIR H +Y+G+
Sbjct: 329 VRGGRARHAASRY------------KVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGV 388

Query: 331 PIRGDVKYEGVTEWNGKI-------------------YDCHELHAESLYFVHPITGIPLK 341
           P+ GD  Y G       +                    D   LHA  L F HP TG  +K
Sbjct: 389 PLLGDEVYGGTKSMALSLLQKRVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVK 416

BLAST of Bhi06G001328 vs. TAIR10
Match: AT3G19440.1 (Pseudouridine synthase family protein)

HSP 1 Score: 63.5 bits (153), Expect = 2.9e-10
Identity = 62/223 (27.80%), Postives = 95/223 (42.60%), Query Frame = 0

Query: 92  VIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPEL-------------HL 151
           V+Y+D  +I +NKP G             L+    +GIKTS+ EL              L
Sbjct: 185 VLYKDPAIIVLNKPHG-------------LAVQGGSGIKTSIDELAASCLKFDKSESPRL 244

Query: 152 ANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRS 211
            +RLDRD SG++V+ ++   A+ L   F + T          G++    K N+KS     
Sbjct: 245 VHRLDRDCSGLLVLARTQTAATVLHSIFREKT---------TGASAYGVKKNVKS----- 304

Query: 212 KFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKS 271
              + R Y A  +G   P     +      +V+  +G++        G+   T    T+ 
Sbjct: 305 ---LKRKYMALVIG--CPPRQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEY 364

Query: 272 LVDIDGQKDEILIRARPRSGRTHQIRLHC-QYLGIPIRGDVKY 301
            V          +  RP +GR HQ+R+HC + LG PI GD KY
Sbjct: 365 RVIESSPHGYTWLELRPLTGRKHQLRVHCAEVLGTPIVGDYKY 375

BLAST of Bhi06G001328 vs. TAIR10
Match: AT3G52260.3 (Pseudouridine synthase family protein)

HSP 1 Score: 55.8 bits (133), Expect = 6.1e-08
Identity = 53/213 (24.88%), Postives = 92/213 (43.19%), Query Frame = 0

Query: 91  DVIYEDEWLIAVNKPQGIYC--------ESVLAAVPRLL-SDSAMAGIKTSLPELHLANR 150
           +V+YED+ LIA+NKP G+           +VL  +      + +  G + S P     +R
Sbjct: 128 EVLYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRES-PHPVPVHR 187

Query: 151 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFG 210
           L R TSG+++  K+    +KL   F + T         VGS      ++ + G GR    
Sbjct: 188 LGRGTSGILLCAKTKLAKTKLAAYFAEGT-------SLVGSG----NLDQECGTGRK--- 247

Query: 211 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVD 270
                  + + R+L  G V  D     + + +     + +       E         +++
Sbjct: 248 ------LSKIYRALADGIVEEDEVVIKQPIGVVRYPGVAQGLYVASPEGKPAFSKVFVLE 307

Query: 271 IDGQKDEILIRARPRSGRTHQIRLHCQYLGIPI 295
            D +K+  L++   +SGR HQIR+H  Y+G P+
Sbjct: 308 RDREKNCSLVKVEIQSGRPHQIRIHLAYMGHPL 319

BLAST of Bhi06G001328 vs. TAIR10
Match: AT5G51140.2 (Pseudouridine synthase family protein)

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-06
Identity = 44/161 (27.33%), Postives = 64/161 (39.75%), Query Frame = 0

Query: 140 NRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSK 199
           +RLDR  SG+++I ++   A    +      V K YIA  +G  P+ + I          
Sbjct: 184 HRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMI---------- 243

Query: 200 FGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSL 259
                V A  +   S  G S   D  +  +   + G+ A  +F +               
Sbjct: 244 -----VDANINYNGS-EGRSTAEDANSSGDDKKVKGKPACTKFTR--------------- 303

Query: 260 VDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKY 301
             ID      L+   P +GRTHQIR+H QY G PI  D  Y
Sbjct: 304 --IDTNGTHSLVSCEPVTGRTHQIRVHLQYTGHPIANDPLY 311

BLAST of Bhi06G001328 vs. TrEMBL
Match: tr|A0A0A0LJY6|A0A0A0LJY6_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 8.9e-179
Identity = 319/359 (88.86%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MALLISLQPTFSTPHKTFTEFP--KPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60
           MAL ISL P FS   KTFTEFP  K FC  RFKS+S SM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSA AGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNA 240
           CVG++PKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NG+N 
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEE QKF + +EET+VV TKSLVDID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 357
           VKYEGVTEWN KIYD HELHAESLYFVHP+TGIPLKLQAPLPSWASQA QPQQ EVNSP
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSP 359

BLAST of Bhi06G001328 vs. TrEMBL
Match: tr|A0A1S3BW87|A0A1S3BW87_CUCME (RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 9.3e-168
Identity = 296/321 (92.21%), Postives = 308/321 (95.95%), Query Frame = 0

Query: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 156
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 157 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLP 216
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI++KSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 217 GGSVVRDMETYFEVLSINGQNAMEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARP 276
           GGS+VRDMETYFEVLS+NG+N MEE QKF K +EET+VV TKSLVDID QKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 277 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQ 336
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YD HELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 337 APLPSWASQAWQPQQQEVNSP 357
           APLPSWASQA QPQQQEVNSP
Sbjct: 301 APLPSWASQALQPQQQEVNSP 321

BLAST of Bhi06G001328 vs. TrEMBL
Match: tr|A0A251NY93|A0A251NY93_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G313700 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.0e-129
Identity = 239/344 (69.48%), Postives = 281/344 (81.69%), Query Frame = 0

Query: 9   PTFSTPHKTFTEFPKPFCSGRFKSISKSMA-DSSEPVTGNCPSTRENYPVPLSPPLPAIS 68
           P FS    T    PK F      SI+ S+  D+  P + N   T +NYPVPLSPPLP IS
Sbjct: 23  PLFSLQPATLFFTPKAFVFLSRSSITMSVQFDAINPSSQNALQTSQNYPVPLSPPLPPIS 82

Query: 69  KNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVPRLLSDSA-- 128
           K++ELARAM+ASSKSSLF+LS +DV++ED+WLI VNKPQG+YCESVL +VP+LL DSA  
Sbjct: 83  KHIELARAMSASSKSSLFSLSRDDVVFEDQWLIVVNKPQGVYCESVLESVPQLLCDSAES 142

Query: 129 MAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPK 188
           +AG   +  ELHLANRLDRDTSG+MVITKSHKVASKLVKAFTDH +SK+YIA C+GSAPK
Sbjct: 143 VAGTPANQLELHLANRLDRDTSGLMVITKSHKVASKLVKAFTDHKISKTYIARCIGSAPK 202

Query: 189 WKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKF 248
           W++I +KSGHGRSKFG WRVYAA+DVGR+LPGGS+VRDMET FEVLSINGQ +++E  +F
Sbjct: 203 WERITLKSGHGRSKFGAWRVYAASDVGRTLPGGSMVRDMETSFEVLSINGQGSLKEPSEF 262

Query: 249 GKDE-ETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVT 308
            KDE  T+VVQ K+++D D +KDEIL+RARPRSGRTHQIRLHCQYLGI IRGDVKYEGV 
Sbjct: 263 KKDEANTIVVQEKAVIDKDTKKDEILVRARPRSGRTHQIRLHCQYLGISIRGDVKYEGVY 322

Query: 309 EWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQP 349
           EW G+ YD HELHAESL F HPITG+P+  QAP+PSWASQA QP
Sbjct: 323 EWKGRTYDGHELHAESLSFEHPITGLPVMFQAPVPSWASQALQP 366

BLAST of Bhi06G001328 vs. TrEMBL
Match: tr|M5VZ97|M5VZ97_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa008866mg PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 5.0e-129
Identity = 228/311 (73.31%), Postives = 268/311 (86.17%), Query Frame = 0

Query: 39  DSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEW 98
           D+  P + N   T +NYPVPLSPPLP ISK++ELARAM+ASSKSSLF+LS +DV++ED+W
Sbjct: 6   DAINPSSQNALQTSQNYPVPLSPPLPPISKHIELARAMSASSKSSLFSLSRDDVVFEDQW 65

Query: 99  LIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKV 158
           LI VNKPQG+YCESVL +VP+LL DSA +G   +  ELHLANRLDRDTSG+MVITKSHKV
Sbjct: 66  LIVVNKPQGVYCESVLESVPQLLCDSAESGTPANQLELHLANRLDRDTSGLMVITKSHKV 125

Query: 159 ASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGG 218
           ASKLVKAFTDH +SK+YIA C+GSAPKW++I +KSGHGRSKFG WRVYAA+DVGR+LPGG
Sbjct: 126 ASKLVKAFTDHKISKTYIARCIGSAPKWERITLKSGHGRSKFGAWRVYAASDVGRTLPGG 185

Query: 219 SVVRDMETYFEVLSINGQNAMEEFQKFGKDE-ETVVVQTKSLVDIDGQKDEILIRARPRS 278
           S+VRDMET FEVLSINGQ +++E  +F KDE  T+VVQ K+++D D +KDEIL+RARPRS
Sbjct: 186 SMVRDMETSFEVLSINGQGSLKEPSEFKKDEANTIVVQEKAVIDKDTKKDEILVRARPRS 245

Query: 279 GRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAP 338
           GRTHQIRLHCQYLGI IRGDVKYEGV EW G+ YD HELHAESL F HPITG+P+  QAP
Sbjct: 246 GRTHQIRLHCQYLGISIRGDVKYEGVYEWKGRTYDGHELHAESLSFEHPITGLPVMFQAP 305

Query: 339 LPSWASQAWQP 349
           +PSWASQA QP
Sbjct: 306 VPSWASQALQP 316

BLAST of Bhi06G001328 vs. TrEMBL
Match: tr|A0A2C9VE03|A0A2C9VE03_MANES (Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G176400 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 3.0e-126
Identity = 229/315 (72.70%), Postives = 261/315 (82.86%), Query Frame = 0

Query: 37  MADSSEPVT---GNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVI 96
           M+ SS P+     N P T +NYP PLSPPLP ISK +ELARAM ASS SSLFALS +D++
Sbjct: 1   MSSSSGPINLAPQNAPLTVQNYPTPLSPPLPPISKQIELARAMTASSISSLFALSRSDIL 60

Query: 97  YEDEWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVIT 156
           YED+WL+AVNKPQGIYCE+VL +VPR L+D +    K +LPELHLANRLDRDTSGV VIT
Sbjct: 61  YEDQWLLAVNKPQGIYCEAVLESVPRFLADLSEEA-KGTLPELHLANRLDRDTSGVTVIT 120

Query: 157 KSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGR 216
           KSHKVASKLVKAFTDH V K+YIA CVGSAPKW+KI IKSGHGRSKFG WRVYAA+DVGR
Sbjct: 121 KSHKVASKLVKAFTDHKVKKTYIALCVGSAPKWEKITIKSGHGRSKFGAWRVYAASDVGR 180

Query: 217 SLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKDEETVVVQTKSLVDIDGQKDEILIRA 276
           +LPGGSVVRDMET FE+LS+NGQ   +E ++F KDE  +VV+ K++ D DG+KDEILIRA
Sbjct: 181 TLPGGSVVRDMETSFELLSVNGQGNFKELREFEKDENVIVVEEKAVKDADGKKDEILIRA 240

Query: 277 RPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLK 336
            P+SGRTHQIRLHCQYLGI IRGDVKYEGV EW  KIYD HELHAESL F HPITG+P+ 
Sbjct: 241 FPQSGRTHQIRLHCQYLGISIRGDVKYEGVYEWKEKIYDGHELHAESLSFEHPITGLPVM 300

Query: 337 LQAPLPSWASQAWQP 349
             APLPSWA +A QP
Sbjct: 301 FCAPLPSWAKEAVQP 314

BLAST of Bhi06G001328 vs. NCBI nr
Match: KGN60992.1 (hypothetical protein Csa_2G033960 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 1.3e-178
Identity = 319/359 (88.86%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MALLISLQPTFSTPHKTFTEFP--KPFCSGRFKSISKSMADSSEPVTGNCPSTRENYPVP 60
           MAL ISL P FS   KTFTEFP  K FC  RFKS+S SM DSSEPVTGNCPST ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAASSKSSLFALSANDVIYEDEWLIAVNKPQGIYCESVLAAVP 120
           LSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYEDEWLIAVNKPQGIYCE+VLAAVP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180
           RLL DSA AGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF
Sbjct: 121 RLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAF 180

Query: 181 CVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNA 240
           CVG++PKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NG+N 
Sbjct: 181 CVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGKNT 240

Query: 241 MEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300
           MEE QKF + +EET+VV TKSLVDID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGD
Sbjct: 241 MEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGD 300

Query: 301 VKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 357
           VKYEGVTEWN KIYD HELHAESLYFVHP+TGIPLKLQAPLPSWASQA QPQQ EVNSP
Sbjct: 301 VKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQQHEVNSP 359

BLAST of Bhi06G001328 vs. NCBI nr
Match: XP_008453651.1 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo])

HSP 1 Score: 598.6 bits (1542), Expect = 1.4e-167
Identity = 296/321 (92.21%), Postives = 308/321 (95.95%), Query Frame = 0

Query: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 156
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKT+LPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAGIKTNLPELHLANRLDRDTSGVMVITKSH 120

Query: 157 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLP 216
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKI++KSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 217 GGSVVRDMETYFEVLSINGQNAMEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARP 276
           GGS+VRDMETYFEVLS+NG+N MEE QKF K +EET+VV TKSLVDID QKDE+LIRARP
Sbjct: 181 GGSIVRDMETYFEVLSVNGKNTMEELQKFRKEEEETIVVHTKSLVDIDSQKDEVLIRARP 240

Query: 277 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQ 336
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGK YD HELHAESLYFVHPITGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPITGIPLKLQ 300

Query: 337 APLPSWASQAWQPQQQEVNSP 357
           APLPSWASQA QPQQQEVNSP
Sbjct: 301 APLPSWASQALQPQQQEVNSP 321

BLAST of Bhi06G001328 vs. NCBI nr
Match: XP_004144814.2 (PREDICTED: RNA pseudouridine synthase 1 [Cucumis sativus])

HSP 1 Score: 594.0 bits (1530), Expect = 3.4e-166
Identity = 294/321 (91.59%), Postives = 305/321 (95.02%), Query Frame = 0

Query: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96
           M DSSEPVTGNCPST ENYPVPLSPPLPAISKNLELARAM ASSKSSL+ALSANDVIYED
Sbjct: 1   MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 60

Query: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAGIKTSLPELHLANRLDRDTSGVMVITKSH 156
           EWLIAVNKPQGIYCE+VLAAVPRLL DSA AGIKTSLPELHLANRLDRDTSGVMVITKSH
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAGIKTSLPELHLANRLDRDTSGVMVITKSH 120

Query: 157 KVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADVGRSLP 216
           KVASKLVKAFTDHTVSKSYIAFCVG++PKWKKIN+KSGHGRSKFGVWRVYAAADVGRSLP
Sbjct: 121 KVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLP 180

Query: 217 GGSVVRDMETYFEVLSINGQNAMEEFQKFGK-DEETVVVQTKSLVDIDGQKDEILIRARP 276
           GGSVVRDMETYFEVLS+NG+N MEE QKF + +EET+VV TKSLVDID  KDEILIRARP
Sbjct: 181 GGSVVRDMETYFEVLSVNGKNTMEELQKFRRGEEETIVVHTKSLVDIDSHKDEILIRARP 240

Query: 277 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGIPLKLQ 336
           RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWN KIYD HELHAESLYFVHP+TGIPLKLQ
Sbjct: 241 RSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQ 300

Query: 337 APLPSWASQAWQPQQQEVNSP 357
           APLPSWASQA QPQQ EVNSP
Sbjct: 301 APLPSWASQALQPQQHEVNSP 321

BLAST of Bhi06G001328 vs. NCBI nr
Match: XP_022156345.1 (RNA pseudouridine synthase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 561.2 bits (1445), Expect = 2.5e-156
Identity = 281/326 (86.20%), Postives = 299/326 (91.72%), Query Frame = 0

Query: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96
           MAD S PVTGN P+T +NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMA-----GIKTSLPELHLANRLDRDTSGVMV 156
           EWLIAVNKPQG+YCESVLA+VPRLL DSAMA     GI+ SLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGIQISLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKD-EETVVVQTKSLVDIDGQKDEIL 276
           GRSLPGGSVVRDMETYFEVLS+NGQ+ M+E  +  KD EET+VVQ+KSL+DID  KDEIL
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLIDIDAHKDEIL 240

Query: 277 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAESLYFVHPITGI 336
           IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG IYD HELHAESL+FVHP+TGI
Sbjct: 241 IRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAESLFFVHPVTGI 300

Query: 337 PLKLQAPLPSWASQAWQPQQQEVNSP 357
           PLKLQAPLPSWASQA   QQQEV+SP
Sbjct: 301 PLKLQAPLPSWASQALLSQQQEVSSP 326

BLAST of Bhi06G001328 vs. NCBI nr
Match: XP_022156344.1 (RNA pseudouridine synthase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 557.4 bits (1435), Expect = 3.6e-155
Identity = 281/336 (83.63%), Postives = 299/336 (88.99%), Query Frame = 0

Query: 37  MADSSEPVTGNCPSTRENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 96
           MAD S PVTGN P+T +NYP+PLSPPLPAISKNLELARAMAASSKSSLFALSA DVI+ED
Sbjct: 1   MADPSIPVTGNSPATGQNYPIPLSPPLPAISKNLELARAMAASSKSSLFALSATDVIFED 60

Query: 97  EWLIAVNKPQGIYCESVLAAVPRLLSDSAMAG---------------IKTSLPELHLANR 156
           EWLIAVNKPQG+YCESVLA+VPRLL DSAMAG               I+ SLPELHLANR
Sbjct: 61  EWLIAVNKPQGVYCESVLASVPRLLGDSAMAGEQSGGTTTLCLCLARIQISLPELHLANR 120

Query: 157 LDRDTSGVMVITKSHKVASKLVKAFTDHTVSKSYIAFCVGSAPKWKKINIKSGHGRSKFG 216
           LDRDTSGVMVITKSHKVASKLVKAFTDH VSKSYIAFCVGSAPKWKKIN+KSGHGRSKFG
Sbjct: 121 LDRDTSGVMVITKSHKVASKLVKAFTDHNVSKSYIAFCVGSAPKWKKINVKSGHGRSKFG 180

Query: 217 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSINGQNAMEEFQKFGKD-EETVVVQTKSLV 276
           VWRVYAAADVGRSLPGGSVVRDMETYFEVLS+NGQ+ M+E  +  KD EET+VVQ+KSL+
Sbjct: 181 VWRVYAAADVGRSLPGGSVVRDMETYFEVLSVNGQSTMDELPEVRKDEEETIVVQSKSLI 240

Query: 277 DIDGQKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKIYDCHELHAES 336
           DID  KDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGV EWNG IYD HELHAES
Sbjct: 241 DIDAHKDEILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVIEWNGNIYDSHELHAES 300

Query: 337 LYFVHPITGIPLKLQAPLPSWASQAWQPQQQEVNSP 357
           L+FVHP+TGIPLKLQAPLPSWASQA   QQQEV+SP
Sbjct: 301 LFFVHPVTGIPLKLQAPLPSWASQALLSQQQEVSSP 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
sp|Q2QNM3|PUS1_ORYSJ4.2e-9961.69RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g05... [more]
sp|Q7XA65|PUS1_ARATH1.7e-9557.67RNA pseudouridine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g56345 PE=2 S... [more]
sp|Q68XB2|RLUC_RICTY9.6e-1932.40Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC... [more]
sp|Q9ZDR7|RLUC_RICPR1.4e-1730.00Ribosomal large subunit pseudouridine synthase C OS=Rickettsia prowazekii (strai... [more]
sp|Q1RJX7|RLUC_RICBR1.2e-1627.07Ribosomal large subunit pseudouridine synthase C OS=Rickettsia bellii (strain RM... [more]
Match NameE-valueIdentityDescription
AT1G56345.19.2e-9757.67Pseudouridine synthase family protein[more]
AT1G76050.24.1e-1223.78Pseudouridine synthase family protein[more]
AT3G19440.12.9e-1027.80Pseudouridine synthase family protein[more]
AT3G52260.36.1e-0824.88Pseudouridine synthase family protein[more]
AT5G51140.24.4e-0627.33Pseudouridine synthase family protein[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LJY6|A0A0A0LJY6_CUCSA8.9e-17988.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033960 PE=4 SV=1[more]
tr|A0A1S3BW87|A0A1S3BW87_CUCME9.3e-16892.21RNA pseudouridine synthase 1 OS=Cucumis melo OX=3656 GN=LOC103494301 PE=4 SV=1[more]
tr|A0A251NY93|A0A251NY93_PRUPE1.0e-12969.48Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G313700 PE=4 SV=1[more]
tr|M5VZ97|M5VZ97_PRUPE5.0e-12973.31Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa008866mg PE=4 SV=1[more]
tr|A0A2C9VE03|A0A2C9VE03_MANES3.0e-12672.70Uncharacterized protein OS=Manihot esculenta OX=3983 GN=MANES_09G176400 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
KGN60992.11.3e-17888.86hypothetical protein Csa_2G033960 [Cucumis sativus][more]
XP_008453651.11.4e-16792.21PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo][more]
XP_004144814.23.4e-16691.59PREDICTED: RNA pseudouridine synthase 1 [Cucumis sativus][more]
XP_022156345.12.5e-15686.20RNA pseudouridine synthase 1 isoform X2 [Momordica charantia][more]
XP_022156344.13.6e-15583.63RNA pseudouridine synthase 1 isoform X1 [Momordica charantia][more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0009982pseudouridine synthase activity
GO:0003723RNA binding
Vocabulary: Biological Process
TermDefinition
GO:0001522pseudouridine synthesis
GO:0009451RNA modification
Vocabulary: INTERPRO
TermDefinition
IPR020103PsdUridine_synth_cat_dom_sf
IPR006224PsdUridine_synth_RluC/D_CS
IPR006145PsdUridine_synth_RsuA/RluD
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
cellular_component GO:0005575 cellular_component
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0016798 hydrolase activity, acting on glycosyl bonds

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi06M001328Bhi06M001328mRNA


Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluB/C/D/E/FPFAMPF00849PseudoU_synth_2coord: 99..288
e-value: 1.5E-23
score: 83.6
IPR006145Pseudouridine synthase, RsuA/RluB/C/D/E/FCDDcd02869PseudoU_synth_RluCD_likecoord: 99..323
e-value: 2.70407E-38
score: 135.543
NoneNo IPR availablePANTHERPTHR45372FAMILY NOT NAMEDcoord: 49..348
NoneNo IPR availablePANTHERPTHR45372:SF2RNA PSEUDOURIDINE SYNTHASE 1coord: 49..348
IPR006224Pseudouridine synthase, RluC/RluD, conserved sitePROSITEPS01129PSI_RLUcoord: 139..153
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILYSSF55120Pseudouridine synthasecoord: 90..233
coord: 265..348

The following gene(s) are paralogous to this gene:

None