CmaCh12G010340 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh12G010340
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Ribosomal large subunit pseudouridine synthase C
Location	Cma_Chr12 : 8207326 .. 8210366 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCTCGTCGTTACACTTCAGCCTCTCTTCTCAATTCCCCACAAATCCTTCGCCAAATTCCCAAAACCCTTCCGCTTCCATAGATTCAGGCCAGTCACCAATAGCATGGCGGACTCATCAAAGCCGGTGACCGGAGAGAATTACCCGGTACCACTCTCGCCTCCATTGCCGGCGATTTCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTGCTCTAAATCCAGCCTATTTTCTTTGTCAGCTTGCGATGTTATATACGAGGATGAGTGGCTCATTGCTGTCAATAAGACTCAAGGGGTATACTGTGAAAGCGTTTTGGGCTCTGTTCCTCGTTTTCTCGGCGATTCGGCTATGGCTGGTGCGCAGAGTGGAGGTACTAGAATTCTCTGCCTATGTTAACTCTTTGCTAGAATTTTTTTGAGACAGATTTAGATTTTAACATGAGCTTGGCAAGTTGAATTCAATTTTACTCAAATTTTCGTATTTGATAATCGATTGGAATGGTGTAAGTGCAACTTTTGATTTGCTTTCGTTTGAAGAATTGTAAAATGATATTAGAATGTGCTTTCAATGGATTTTCAAATATGGACGAAGCTGCAAAGAATGAAGATATAAGTGTTGAATTGTTCTTAAAGATAGTTTTGAAAGATTTATTCCATACAGATCTCGGGCATTGATTTCTTTAAACTTTTGTGCATTAATATTGTTGAAATTGGGTGAAATTAATAGATAAATGTAATGGAAGTCTAACAAATTGTATCAATGAACTGTTTTCTGGAATTTGTATTCATTGTTTTAAGCTGTTTAAGAACGAAATCTCTGAAATTCTCATCCCTTTTGTATTTTTCATGAAAAGTTGTCGTCTAATGGTTTTTAAGGAGAGAGAGAAAATGTAACGACTCAAGCCCGCTAAAAGATATGGCCGTCTTTGGGCTTCCCTTCAAGGGTTTTAAAACGCGTATGCTAGGGAGAGGTTTCTACACCTTATAAGGGTGTTTCGTTCTCCTCCCCAACTGATATGGGATCTCACAATCCACCCCTCTTCAGGCCCTAGCGTCTTCTCTGGCACTCGTTTTCTTCTCCAATCGATGTGGGACCCCCCAAATCCACCCCCTTTAGGTCTAGCATCCTTGCTAGCACACCGCCTCGTGTCCACCTCCCTTCGGTGACTCAACTTTCTCGTTGGCACATCGTCCGGTGCTGCCTCTAATACTATTTGTAACTGCCCAAGCCCACAACTAGCATATATTATCCTCTTTGAGCTTTCCCTCAAAGTTTTTAAAACGCGTCTGATAGGGAGAGGGTTTCACACCCTTGTAAAAGGTGTTTCGTTCTCCTCCTTAACCAACGTGGGATCTCACATAAAAACCTAAAACAAACCTATGTTTTTTCAACTAAATTGTCAAATAAGCCTTTAGTTTATCTCATCAAATTATGTCAGTAAAGTTGTCTGGGAGTTTGATATGTGTACATTTACTTAAGATTTGTGTAATTGTTTGGTGCTTCTTTCCCTACATTGCCAAAAATAGCAATGTTTCTGATCATTAGACAGTCGATATAAATGGTTTAGCTATATCCTGGTTGTTGAAGAGAGATCTGCTTGAATGTATCTGAAGTATTAAACTTTTGTCTAAAATGATATCATGTAATCTTTCGATTATTCTATCACTTTCGATTTATCGGATTGTCCTCTGCCCGGATTCTTTATATGCAGAGATCAAAATCAGTCTGCCAGAGCTTCATCTTGCCAATCGTCTCGATCGTGACACAAGCGGAGTGATGGTTATAACGAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGTTCGGCTCCAAAATGGAAAAAAATCATTGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGAGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGCTCCTTACCGGGCGGTTCGGTAGTCCGAGACATGGAAACCTCTTTTGAAGTATTGTCTGTAAATGGGCAAAGCACCAAAGAGGAACTTCCAGAATCTAGAAAAGATGAAGAAGAAGAAGAAGAAACAATTGTAGCCCAATCAAAATCTCTGATAGATATCGATGCTCGGAAGGACGAGATATTGATTCGAGCACGACCTCGGAGTGGAAGGACTCATCAAATCCGTCTTCACTCCCAATATCTTGGAATTCCTATTGTAGGAGATGTGAAATATGAAGGTGTTACGGAGTGGAATGGAAAAATTTATGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTATTACAGGCATTCCTCTCAAACTGCAAGCTCCTCTGCCATCATGGGCCACCCAAGCATTGCAGCCCCAACAGCAAGAAATGAATTCTGCAGCATCCTTGAAACCTATGCCTTAACATTATCACTGGCACTGTTATACGATGAGTCTCAGTGTCGAAGAATGTCGGGAAGCGGTTAGTATTCAATCTAATTCGTACAAGTTGAACTACAGAGTTTTCGTATCACTGACTTCCTGTCTGCATTTTCCTCGACAGGTGCTCGTTGAATCAGACGTTAGATGAGCTCTTGAGAGCACAACAGTCTCCTCTAGAATATCAGCAAAGTCGGGAACGAACAACTTGCTGAGTTTCTTCTCAAAAATCGAAAGGATGGGATGCTCGTAGCCGATTCTGGACATGAAAGAAGAAGCATGGCATGTCTAAGGCGGTCGAGGTTCTTCAAAGCACCCTCGGAAACTTTCCCCTTCCCTATATTTGTGCAATTATATGATAAATCGTTTAGAAATCGAATGTCTTTATAAAAATATAGATTGAATTGTTATATAATAGAATCGTATCGATATTTCACCTCGATGATTGTCCTTCCATCACAACCAGAAGTTGCAATAGATTTGAATAACTGTGTACAAGTGGACAATAAATTAGACATGAATGAACCTTAAATGCTAAGGAAAGGAAGTTGTTGAAGGAAGTCATTGAACCTTCAGTTTTTTTTA

mRNA sequence

ATGGCCCTCGTCGTTACACTTCAGCCTCTCTTCTCAATTCCCCACAAATCCTTCGCCAAATTCCCAAAACCCTTCCGCTTCCATAGATTCAGGCCAGTCACCAATAGCATGGCGGACTCATCAAAGCCGGTGACCGGAGAGAATTACCCGGTACCACTCTCGCCTCCATTGCCGGCGATTTCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTGCTCTAAATCCAGCCTATTTTCTTTGTCAGCTTGCGATGTTATATACGAGGATGAGTGGCTCATTGCTGTCAATAAGACTCAAGGGGTATACTGTGAAAGCGTTTTGGGCTCTGTTCCTCGTTTTCTCGGCGATTCGGCTATGGCTGGTGCGCAGAGTGGAGAGATCAAAATCAGTCTGCCAGAGCTTCATCTTGCCAATCGTCTCGATCGTGACACAAGCGGAGTGATGGTTATAACGAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGTTCGGCTCCAAAATGGAAAAAAATCATTGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGAGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGCTCCTTACCGGGCGGTTCGGTAGTCCGAGACATGGAAACCTCTTTTGAAGTATTGTCTGTAAATGGGCAAAGCACCAAAGAGGAACTTCCAGAATCTAGAAAAGATGAAGAAGAAGAAGAAGAAACAATTGTAGCCCAATCAAAATCTCTGATAGATATCGATGCTCGGAAGGACGAGATATTGATTCGAGCACGACCTCGGAGTGGAAGGACTCATCAAATCCGTCTTCACTCCCAATATCTTGGAATTCCTATTGTAGGAGATGTGAAATATGAAGGTGTTACGGAGTGGAATGGAAAAATTTATGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTATTACAGGCATTCCTCTCAAACTGCAAGCTCCTCTGCCATCATGGGCCACCCAAGCATTGCAGCCCCAACAGCAAGAAATGAATTCTGCAGCATCCTTGAAACCTATGCCTTAACATTATCACTGGCACTGTTATACGATGAGTCTCAGTGTCGAAGAATGTCGGGAAGCGGTGCTCGTTGAATCAGACGTTAGATGAGCTCTTGAGAGCACAACAGTCTCCTCTAGAATATCAGCAAAGTCGGGAACGAACAACTTGCTGAGTTTCTTCTCAAAAATCGAAAGGATGGGATGCTCGTAGCCGATTCTGGACATGAAAGAAGAAGCATGGCATGTCTAAGGCGGTCGAGGTTCTTCAAAGCACCCTCGGAAACTTTCCCCTTCCCTATATTTGTGCAATTATATGATAAATCGTTTAGAAATCGAATGTCTTTATAAAAATATAGATTGAATTGTTATATAATAGAATCGTATCGATATTTCACCTCGATGATTGTCCTTCCATCACAACCAGAAGTTGCAATAGATTTGAATAACTGTGTACAAGTGGACAATAAATTAGACATGAATGAACCTTAAATGCTAAGGAAAGGAAGTTGTTGAAGGAAGTCATTGAACCTTCAGTTTTTTTTA

Coding sequence (CDS)

ATGGCCCTCGTCGTTACACTTCAGCCTCTCTTCTCAATTCCCCACAAATCCTTCGCCAAATTCCCAAAACCCTTCCGCTTCCATAGATTCAGGCCAGTCACCAATAGCATGGCGGACTCATCAAAGCCGGTGACCGGAGAGAATTACCCGGTACCACTCTCGCCTCCATTGCCGGCGATTTCCAAGAACTTGGAGCTCGCTAGAGCCATGGCTGCCTGCTCTAAATCCAGCCTATTTTCTTTGTCAGCTTGCGATGTTATATACGAGGATGAGTGGCTCATTGCTGTCAATAAGACTCAAGGGGTATACTGTGAAAGCGTTTTGGGCTCTGTTCCTCGTTTTCTCGGCGATTCGGCTATGGCTGGTGCGCAGAGTGGAGAGATCAAAATCAGTCTGCCAGAGCTTCATCTTGCCAATCGTCTCGATCGTGACACAAGCGGAGTGATGGTTATAACGAAGTCGCATAAAGTTGCTTCTAAATTAGTGAAGGCATTTACTGACCACAAGGTTTCAAAATCATATATTGCCTTCTGCGTTGGTTCGGCTCCAAAATGGAAAAAAATCATTGTCAAATCTGGTCATGGAAGGTCAAAGTTCGGAGTCTGGCGTGTCTATGCTGCAGCTGATGTGGGTCGCTCCTTACCGGGCGGTTCGGTAGTCCGAGACATGGAAACCTCTTTTGAAGTATTGTCTGTAAATGGGCAAAGCACCAAAGAGGAACTTCCAGAATCTAGAAAAGATGAAGAAGAAGAAGAAGAAACAATTGTAGCCCAATCAAAATCTCTGATAGATATCGATGCTCGGAAGGACGAGATATTGATTCGAGCACGACCTCGGAGTGGAAGGACTCATCAAATCCGTCTTCACTCCCAATATCTTGGAATTCCTATTGTAGGAGATGTGAAATATGAAGGTGTTACGGAGTGGAATGGAAAAATTTATGATAGCCATGAGCTTCATGCTGAAAGCTTGTATTTTGTGCACCCTATTACAGGCATTCCTCTCAAACTGCAAGCTCCTCTGCCATCATGGGCCACCCAAGCATTGCAGCCCCAACAGCAAGAAATGAATTCTGCAGCATCCTTGAAACCTATGCCTTAA

Protein sequence

MALVVTLQPLFSIPHKSFAKFPKPFRFHRFRPVTNSMADSSKPVTGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQPQQQEMNSAASLKPMP

Homology

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BLAST of CmaCh12G010340 vs. Swiss-Prot
Match: PUS1_ARATH (RNA pseudouridine synthase 1 OS=Arabidopsis thaliana GN=At1g56345 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.1e-94
Identity = 180/300 (60.00%), Postives = 220/300 (73.33%), Query Frame = 1


Query: 48  NYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESV 107
           NYP P+S P P ISK++EL RAM A SKSSLF+L+  D++YEDE+L+AVNK +GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 108 LGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 167
           L S P+ + DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSSS-------------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 168 HKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSF 227
           HK+ KSYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET+F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 228 EVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDE--ILIRARPRSGRTHQ 287
           EV+SVN  S K E  E     E+    IVA+ +  +      D+  +++RA PRSGRTHQ
Sbjct: 219 EVVSVN--SVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQ 278

Query: 288 IRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWA 346
           IRLH QYLGIPI GDVKY GV EWNG+ ++ HELHAE L   HP+TG  + ++APLP WA
Sbjct: 279 IRLHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWA 319

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BLAST of CmaCh12G010340 vs. Swiss-Prot
Match: PUS1_ORYSJ (RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica GN=Os12g0560500 PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-92
Identity = 180/309 (58.25%), Postives = 220/309 (71.20%), Query Frame = 1


Query: 45  TGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYC 104
           TGE YPVP+SPP PA SK++EL RAM A ++S+ +S  +  V++EDEWL  V+K  GVYC
Sbjct: 48  TGE-YPVPVSPPYPAASKDVELRRAMTASARSAAYS--SAPVVFEDEWLAVVDKPAGVYC 107

Query: 105 ESVLGSVP---RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKL 164
           +++L ++P     LGD A           + P LHLANRLDRDTSG+MVITK +KVA KL
Sbjct: 108 DALLSALPCSAATLGDEA-----------TKPNLHLANRLDRDTSGLMVITKCNKVAGKL 167

Query: 165 VKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVR 224
           VKAFT+HKV K+Y+A C+G  P W+KI + SGHGRSK G WRVYA +DVGRSLPGGSVVR
Sbjct: 168 VKAFTEHKVKKTYLALCIGYPPAWEKIKICSGHGRSKHGAWRVYAMSDVGRSLPGGSVVR 227

Query: 225 DMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKS---LIDIDARKDEILIRARP 284
           DM T FEVL +NG   K +  E    E +E E+I  Q K+     D D +   IL+RA P
Sbjct: 228 DMSTRFEVLGING---KGQFREPSNFEVDETESITVQEKAADLTSDGDEKNSIILVRAYP 287

Query: 285 RSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQ 344
           +SGRTHQIRLH QYLG PI GDVKY GV EWNG  YD H LHAESL FVHP+TG+P+  +
Sbjct: 288 QSGRTHQIRLHCQYLGFPIRGDVKYSGVIEWNGVDYDGHALHAESLSFVHPVTGLPVTFR 339

Query: 345 APLPSWATQ 348
           +PLPSWA +
Sbjct: 348 SPLPSWANE 339

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BLAST of CmaCh12G010340 vs. Swiss-Prot
Match: RLUC_RICTY (Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) GN=rluC PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.2e-14
Identity = 76/268 (28.36%), Postives = 122/268 (45.52%), Query Frame = 1


Query: 86  VIYEDEWLIAVNKTQGVYCESVLGSVPRFLGDSAMA--GAQSGEIKISLPELHLANRLDR 145
           +IYED+ LIA+NK   +  +   GS      DSA+     Q  + K       L +RLD+
Sbjct: 91  LIYEDDHLIAINKPASLATQG--GSKINLSIDSALKYLNYQGADFK-------LVHRLDK 150

Query: 146 DTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWR 205
           +TSG+++I K++  + KL  AF +  V K Y A             +  G      G+ +
Sbjct: 151 ETSGLLLIAKNYLSSVKLHDAFKEKLVIKKYFA-------------ITYGRPVKNVGIVK 210

Query: 206 VYAAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLI 265
               +++G+S      + D+++    L++                     T     KSL 
Sbjct: 211 ----SNIGKSKGRMFKITDIDSDNGKLAI---------------------TYYKLLKSL- 270

Query: 266 DIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKY--EGVTEWNGKIYDSHELHA 325
                 +  LI   P +GR HQ+RLH+Q LG PI+GD KY  + V  ++  ++    LHA
Sbjct: 271 ----NNNLFLIEFMPVTGRMHQLRLHAQLLGCPILGDDKYGNKEVMPYSKYMF----LHA 302

Query: 326 ESLYFVHPITGIPLKLQAPLPSWATQAL 350
            ++Y    I G  +KL+A LP + T+ L
Sbjct: 331 NNIYLSESIVGKEIKLEAKLPFYFTRRL 302

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BLAST of CmaCh12G010340 vs. Swiss-Prot
Match: RLUD_XANAC (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) GN=rluD PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.1e-13
Identity = 70/287 (24.39%), Postives = 126/287 (43.90%), Query Frame = 1


Query: 86  VIYEDEWLIAVNKTQGVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDT 145
           V+YEDE ++ ++K  G+      G+  R L ++ +    +     S+P   + +RLD+DT
Sbjct: 92  VLYEDEQVLVIDKPAGLVVHPGAGNPDRTLVNALLFRDPA---LASVPRAGVVHRLDKDT 151

Query: 146 SGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVY 205
           SGVMV+ ++ +  + LV+  +  +V + Y+A                             
Sbjct: 152 SGVMVVARTVQAQTALVEQLSAREVHRQYLAVV--------------------------- 211

Query: 206 AAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDI 265
               VG  + GG+    ++          +  ++ L  + +D+  +  T     +     
Sbjct: 212 ----VGALVSGGTANAPID----------RHPRDRLKMAVRDDGRDAVTHYRLRERF--- 271

Query: 266 DARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDS--HEL---- 325
              +    +  R  +GRTHQIR+H  +L  PIVGD  Y G  +      D   HEL    
Sbjct: 272 ---RAHTALECRLETGRTHQIRVHMAHLKSPIVGDPLYGGALKLPKGATDELVHELRTFK 328

Query: 326 ----HAESLYFVHPITGIPLKLQAPLPSWATQALQPQQQEMNSAASL 363
               HAE+L F+HP++G P++  AP+P+   + +   +++   AA L
Sbjct: 332 RQALHAETLEFLHPVSGEPVRASAPVPADLQRLMTVLREDSARAAEL 328

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BLAST of CmaCh12G010340 vs. Swiss-Prot
Match: RLUD_VIBPA (Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) GN=rluD PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.0e-13
Identity = 79/294 (26.87%), Postives = 116/294 (39.46%), Query Frame = 1


Query: 85  DVIYEDEWLIAVNKTQ--------GVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELH 144
           D++YED+ +I +NK +        G    +VL ++     D A            +P   
Sbjct: 85  DIVYEDDDIIVINKPRDFVVHPGAGTPDGTVLNALLHHYPDIA-----------EVPRAG 144

Query: 145 LANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGR 204
           + +RLD+DT+G+MV+ K+    ++LV+A                                
Sbjct: 145 IVHRLDKDTTGLMVVAKTVPAQTRLVRAL------------------------------- 204

Query: 205 SKFGVWRVYAAADVGRSLPGGSVVRDM--ETSFEVLSVNGQSTKEELPESRKDEEEEEET 264
            K  + R Y A  +GR   GG V + +   ++   L       K  +   R  E   E T
Sbjct: 205 QKRNITREYEAIAIGRMTAGGKVDQPIGRHSTKRTLMAVAPLGKPAVTHYRVAEHFREHT 264

Query: 265 IVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEG--------V 324
                              IR R  +GRTHQIR+H  YL  P++GD  Y G         
Sbjct: 265 ------------------RIRLRLETGRTHQIRVHMSYLQHPLLGDTAYGGRARIPTGAS 318

Query: 325 TEWNGKI--YDSHELHAESLYFVHPITGIPLKLQAPLPS---WATQALQPQQQE 356
            E    I  +D   LHA  L F HPITG  L+  AP+P      T+AL+   +E
Sbjct: 325 QELTDMIRGFDRQALHAVMLRFEHPITGEELEFHAPVPDDMVAMTEALRKDTEE 318

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BLAST of CmaCh12G010340 vs. TrEMBL
Match: A0A0A0LJY6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G033960 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 9.7e-159
Identity = 298/374 (79.68%), Postives = 322/374 (86.10%), Query Frame = 1


Query: 1   MALVVTLQPLFSIPHKSFAKFP--KPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 60
           MAL ++L P FSI  K+F +FP  K F FHRF+ V+NSM DSS+PVTG      ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVP 120
           LSPPLPAISKNLELARAM A SKSSL++LSA DVIYEDEWLIAVNK QG+YCE+VL +VP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           R LGDSA AG     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 RLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180

Query: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 181 SYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240

Query: 241 NGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQY 300
           NG++T EEL + R+ EEE   TIV  +KSL+DID+ KDEILIRARPRSGRTHQIRLH QY
Sbjct: 241 NGKNTMEELQKFRRGEEE---TIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQY 300

Query: 301 LGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQPQ 360
           LGIPI GDVKYEGVTEWN KIYDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QALQPQ
Sbjct: 301 LGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQ 360

Query: 361 QQEMNSAASLKPMP 367
           Q E+NS  S K MP
Sbjct: 361 QHEVNSPQSFKTMP 366

[Close]

BLAST of CmaCh12G010340 vs. TrEMBL
Match: M5VZ97_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008866mg PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 3.0e-123
Identity = 225/307 (73.29%), Postives = 260/307 (84.69%), Query Frame = 1


Query: 45  TGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYC 104
           T +NYPVPLSPPLP ISK++ELARAM+A SKSSLFSLS  DV++ED+WLI VNK QGVYC
Sbjct: 18  TSQNYPVPLSPPLPPISKHIELARAMSASSKSSLFSLSRDDVVFEDQWLIVVNKPQGVYC 77

Query: 105 ESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 164
           ESVL SVP+ L DSA +G  + ++     ELHLANRLDRDTSG+MVITKSHKVASKLVKA
Sbjct: 78  ESVLESVPQLLCDSAESGTPANQL-----ELHLANRLDRDTSGLMVITKSHKVASKLVKA 137

Query: 165 FTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDME 224
           FTDHK+SK+YIA C+GSAPKW++I +KSGHGRSKFG WRVYAA+DVGR+LPGGS+VRDME
Sbjct: 138 FTDHKISKTYIARCIGSAPKWERITLKSGHGRSKFGAWRVYAASDVGRTLPGGSMVRDME 197

Query: 225 TSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTH 284
           TSFEVLS+NGQ + +E  E +KDE     TIV Q K++ID D +KDEIL+RARPRSGRTH
Sbjct: 198 TSFEVLSINGQGSLKEPSEFKKDEAN---TIVVQEKAVIDKDTKKDEILVRARPRSGRTH 257

Query: 285 QIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSW 344
           QIRLH QYLGI I GDVKYEGV EW G+ YD HELHAESL F HPITG+P+  QAP+PSW
Sbjct: 258 QIRLHCQYLGISIRGDVKYEGVYEWKGRTYDGHELHAESLSFEHPITGLPVMFQAPVPSW 316

Query: 345 ATQALQP 352
           A+QALQP
Sbjct: 318 ASQALQP 316

[Close]

BLAST of CmaCh12G010340 vs. TrEMBL
Match: A0A061F4Q6_THECC (Pseudouridine synthase family protein isoform 1 OS=Theobroma cacao GN=TCM_026756 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-122
Identity = 228/351 (64.96%), Postives = 274/351 (78.06%), Query Frame = 1


Query: 9   PLFSIPHKSF--------AKFPKPFRFHRFRPVTNSMADSSKPVTGENYPVPLSPPLPAI 68
           P FS+P +SF        +KFPK   F   +  TN       P + +NYP PLSPPLP+I
Sbjct: 2   PFFSLPQQSFFLCCPKLLSKFPKTLLFLCPKMSTN-------PNSHQNYPTPLSPPLPSI 61

Query: 69  SKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVPRFLGDSAM 128
           SK++ELARAM A SKSSLF LS  D++YEDEWLIAVNK QG+YC+SVL S+PRFL DS +
Sbjct: 62  SKDIELARAMTASSKSSLFELSRSDILYEDEWLIAVNKPQGIYCDSVLASIPRFLSDSTV 121

Query: 129 AGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVG 188
           +   S   + S  ELHLANRLDRDTSG+MVITKSHKVA+ LVKAFT+H V K+Y+AFC+G
Sbjct: 122 SDRLSDGNQASPFELHLANRLDRDTSGIMVITKSHKVAAALVKAFTNHMVHKTYVAFCIG 181

Query: 189 SAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEE 248
           SAPK ++I +KSGHGRSKFG WRVYAA DVGR LPGGS+VRDM T+FEVLS+NGQ + +E
Sbjct: 182 SAPKSERITIKSGHGRSKFGAWRVYAAGDVGRKLPGGSIVRDMGTTFEVLSINGQQSYKE 241

Query: 249 LPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGD 308
             ES KD   EE +++ + K++ID+DA+KDEIL+RA PRSGRTHQIRLH QYLGIPI GD
Sbjct: 242 PSESVKD---EETSVIVEEKAVIDVDAQKDEILVRAFPRSGRTHQIRLHCQYLGIPIRGD 301

Query: 309 VKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQP 352
           VKYEGV EW G+ YD+HELHAESL F HP+TG P+ +QAPLP WA+QALQP
Sbjct: 302 VKYEGVYEWKGRRYDAHELHAESLSFQHPVTGFPVMIQAPLPLWASQALQP 342

[Close]

BLAST of CmaCh12G010340 vs. TrEMBL
Match: A0A061F426_THECC (Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026756 PE=4 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-120
Identity = 216/312 (69.23%), Postives = 259/312 (83.01%), Query Frame = 1


Query: 40  SSKPVTGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKT 99
           S+ P + +NYP PLSPPLP+ISK++ELARAM A SKSSLF LS  D++YEDEWLIAVNK 
Sbjct: 2   STNPNSHQNYPTPLSPPLPSISKDIELARAMTASSKSSLFELSRSDILYEDEWLIAVNKP 61

Query: 100 QGVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVAS 159
           QG+YC+SVL S+PRFL DS ++   S   + S  ELHLANRLDRDTSG+MVITKSHKVA+
Sbjct: 62  QGIYCDSVLASIPRFLSDSTVSDRLSDGNQASPFELHLANRLDRDTSGIMVITKSHKVAA 121

Query: 160 KLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSV 219
            LVKAFT+H V K+Y+AFC+GSAPK ++I +KSGHGRSKFG WRVYAA DVGR LPGGS+
Sbjct: 122 ALVKAFTNHMVHKTYVAFCIGSAPKSERITIKSGHGRSKFGAWRVYAAGDVGRKLPGGSI 181

Query: 220 VRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPR 279
           VRDM T+FEVLS+NGQ + +E  ES KDEE    +++ + K++ID+DA+KDEIL+RA PR
Sbjct: 182 VRDMGTTFEVLSINGQQSYKEPSESVKDEET---SVIVEEKAVIDVDAQKDEILVRAFPR 241

Query: 280 SGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQA 339
           SGRTHQIRLH QYLGIPI GDVKYEGV EW G+ YD+HELHAESL F HP+TG P+ +QA
Sbjct: 242 SGRTHQIRLHCQYLGIPIRGDVKYEGVYEWKGRRYDAHELHAESLSFQHPVTGFPVMIQA 301

Query: 340 PLPSWATQALQP 352
           PLP WA+QALQP
Sbjct: 302 PLPLWASQALQP 310

[Close]

BLAST of CmaCh12G010340 vs. TrEMBL
Match: A0A067GSR2_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018524mg PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.4e-120
Identity = 231/348 (66.38%), Postives = 267/348 (76.72%), Query Frame = 1


Query: 6   TLQPLFSIPHKSFAKFPKPFRFHRFRPVTNSMADSSKPVTGENYPVPLSPPLPAISKNLE 65
           +  P F++  K+  +F K F               + P T +NYP PLSPPLPAISK++E
Sbjct: 6   SFHPTFTLTPKAVPRFRKTFFSLASMSNPTEPTSQNAPQTQQNYPTPLSPPLPAISKDIE 65

Query: 66  LARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVPRFL-GDSA--MAG 125
           L RAM+A SKSSL+ LS  DV+YEDEWL+AVNK QGVYCESVL SVP  L GDSA   +G
Sbjct: 66  LTRAMSASSKSSLYKLSRSDVLYEDEWLVAVNKPQGVYCESVLASVPCLLAGDSAESKSG 125

Query: 126 AQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVGSA 185
             S   K++L ELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK+YIA C+G A
Sbjct: 126 RLSEGTKLNLLELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKTYIALCIGPA 185

Query: 186 PKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEELP 245
           PKWK+I + SGHGRSKFG WRVYAA+DVGR LPGGSVVRDM TSFEVLSVNG+ + +E  
Sbjct: 186 PKWKRITIISGHGRSKFGAWRVYAASDVGRVLPGGSVVRDMVTSFEVLSVNGEGSFKEPS 245

Query: 246 ESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVK 305
           E    +E EE  IV + K+++D DA+K+EIL+RA PRSGRTHQIRLH QYLGI I GDVK
Sbjct: 246 EL---QEHEENNIVVEKKAVLDADAKKNEILVRAFPRSGRTHQIRLHCQYLGISIRGDVK 305

Query: 306 YEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQ 351
           YEGV EWNGK YD HELHAESL F HP+TG P+ +QAPLP W T+ALQ
Sbjct: 306 YEGVYEWNGKRYDGHELHAESLSFDHPVTGCPVMVQAPLPLWGTEALQ 350

[Close]

BLAST of CmaCh12G010340 vs. TAIR10
Match: AT1G56345.1 (AT1G56345.1 Pseudouridine synthase family protein)

HSP 1 Score: 345.5 bits (885), Expect = 4.0e-95
Identity = 180/300 (60.00%), Postives = 220/300 (73.33%), Query Frame = 1


Query: 48  NYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESV 107
           NYP P+S P P ISK++EL RAM A SKSSLF+L+  D++YEDE+L+AVNK +GVYCE+V
Sbjct: 39  NYPKPISAPPPPISKDIELRRAMEASSKSSLFNLTRDDILYEDEYLMAVNKPKGVYCEAV 98

Query: 108 LGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTD 167
           L S P+ + DS+              E HLANRLDRDTSGVM+ITKSHKVA+KLVKAFT+
Sbjct: 99  LRSAPQIVLDSSS-------------EYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTE 158

Query: 168 HKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSF 227
           HK+ KSYIA C+GS+P W+++ V SGHGRSK G WRVYAA DVGR LPGGS VRDMET+F
Sbjct: 159 HKIRKSYIALCIGSSPNWRRVTVSSGHGRSKHGAWRVYAALDVGRVLPGGSFVRDMETTF 218

Query: 228 EVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDE--ILIRARPRSGRTHQ 287
           EV+SVN  S K E  E     E+    IVA+ +  +      D+  +++RA PRSGRTHQ
Sbjct: 219 EVVSVN--SVKNESCEL----EDVNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQ 278

Query: 288 IRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWA 346
           IRLH QYLGIPI GDVKY GV EWNG+ ++ HELHAE L   HP+TG  + ++APLP WA
Sbjct: 279 IRLHCQYLGIPIRGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWA 319

[Close]

BLAST of CmaCh12G010340 vs. TAIR10
Match: AT1G76050.2 (AT1G76050.2 Pseudouridine synthase family protein)

HSP 1 Score: 53.1 bits (126), Expect = 4.1e-07
Identity = 35/106 (33.02%), Postives = 47/106 (44.34%), Query Frame = 1


Query: 257 AQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIY-- 316
           A    +I+  A     L+  R  +GRTHQIR H++Y+G+P++GD  Y G       +   
Sbjct: 311 ASRYKVIETFAGGGSALVEWRLETGRTHQIRAHAKYMGVPLLGDEVYGGTKSMALSLLQK 370

Query: 317 -----DSHE------------LHAESLYFVHPITGIPLKLQAPLPS 344
                D  E            LHA  L F HP TG  +K   P PS
Sbjct: 371 RVSRSDQEEIIELISRMDRPCLHAIVLGFTHPCTGEIVKFSCPPPS 416

[Close]

BLAST of CmaCh12G010340 vs. NCBI nr
Match: gi|700205873|gb|KGN60992.1| (hypothetical protein Csa_2G033960 [Cucumis sativus])

HSP 1 Score: 567.8 bits (1462), Expect = 1.4e-158
Identity = 298/374 (79.68%), Postives = 322/374 (86.10%), Query Frame = 1


Query: 1   MALVVTLQPLFSIPHKSFAKFP--KPFRFHRFRPVTNSMADSSKPVTG------ENYPVP 60
           MAL ++L P FSI  K+F +FP  K F FHRF+ V+NSM DSS+PVTG      ENYPVP
Sbjct: 1   MALFISLHPNFSISPKTFTEFPFTKSFCFHRFKSVSNSMTDSSEPVTGNCPSTAENYPVP 60

Query: 61  LSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVP 120
           LSPPLPAISKNLELARAM A SKSSL++LSA DVIYEDEWLIAVNK QG+YCE+VL +VP
Sbjct: 61  LSPPLPAISKNLELARAMVASSKSSLYALSANDVIYEDEWLIAVNKPQGIYCENVLAAVP 120

Query: 121 RFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSK 180
           R LGDSA AG     IK SLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDH VSK
Sbjct: 121 RLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHTVSK 180

Query: 181 SYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSV 240
           SYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMET FEVLSV
Sbjct: 181 SYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETYFEVLSV 240

Query: 241 NGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQY 300
           NG++T EEL + R+ EEE   TIV  +KSL+DID+ KDEILIRARPRSGRTHQIRLH QY
Sbjct: 241 NGKNTMEELQKFRRGEEE---TIVVHTKSLVDIDSHKDEILIRARPRSGRTHQIRLHCQY 300

Query: 301 LGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQPQ 360
           LGIPI GDVKYEGVTEWN KIYDSHELHAESLYFVHP+TGIPLKLQAPLPSWA+QALQPQ
Sbjct: 301 LGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPVTGIPLKLQAPLPSWASQALQPQ 360

Query: 361 QQEMNSAASLKPMP 367
           Q E+NS  S K MP
Sbjct: 361 QHEVNSPQSFKTMP 366

[Close]

BLAST of CmaCh12G010340 vs. NCBI nr
Match: gi|659070151|ref|XP_008453651.1| (PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo])

HSP 1 Score: 536.6 bits (1381), Expect = 3.5e-149
Identity = 279/336 (83.04%), Postives = 298/336 (88.69%), Query Frame = 1


Query: 37  MADSSKPVTG------ENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYED 96
           M DSS+PVTG      ENYPVPLSPPLPAISKNLELARAMAA SKSSLF+LSA DVIYED
Sbjct: 1   MTDSSEPVTGNCPSTGENYPVPLSPPLPAISKNLELARAMAASSKSSLFALSANDVIYED 60

Query: 97  EWLIAVNKTQGVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMV 156
           EWLIAVNK QG+YCE+VL +VPR L DSA AG     IK +LPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLCDSANAG-----IKTNLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKIHVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKD 276
           GRSLPGGS+VRDMET FEVLSVNG++T EEL + RK   EEEETIV  +KSL+DID++KD
Sbjct: 181 GRSLPGGSIVRDMETYFEVLSVNGKNTMEELQKFRK---EEEETIVVHTKSLVDIDSQKD 240

Query: 277 EILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPI 336
           E+LIRARPRSGRTHQIRLH QYLGIPI GDVKYEGVTEWNGK YDSHELHAESLYFVHPI
Sbjct: 241 EVLIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNGKTYDSHELHAESLYFVHPI 300

Query: 337 TGIPLKLQAPLPSWATQALQPQQQEMNSAASLKPMP 367
           TGIPLKLQAPLPSWA+QALQPQQQE+NS  S K MP
Sbjct: 301 TGIPLKLQAPLPSWASQALQPQQQEVNSPQSFKTMP 328

[Close]

BLAST of CmaCh12G010340 vs. NCBI nr
Match: gi|778667027|ref|XP_004144814.2| (PREDICTED: RNA pseudouridine synthase 1 [Cucumis sativus])

HSP 1 Score: 532.3 bits (1370), Expect = 6.5e-148
Identity = 277/336 (82.44%), Postives = 295/336 (87.80%), Query Frame = 1


Query: 37  MADSSKPVTG------ENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYED 96
           M DSS+PVTG      ENYPVPLSPPLPAISKNLELARAM A SKSSL++LSA DVIYED
Sbjct: 1   MTDSSEPVTGNCPSTAENYPVPLSPPLPAISKNLELARAMVASSKSSLYALSANDVIYED 60

Query: 97  EWLIAVNKTQGVYCESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMV 156
           EWLIAVNK QG+YCE+VL +VPR LGDSA AG     IK SLPELHLANRLDRDTSGVMV
Sbjct: 61  EWLIAVNKPQGIYCENVLAAVPRLLGDSANAG-----IKTSLPELHLANRLDRDTSGVMV 120

Query: 157 ITKSHKVASKLVKAFTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADV 216
           ITKSHKVASKLVKAFTDH VSKSYIAFCVG++PKWKKI VKSGHGRSKFGVWRVYAAADV
Sbjct: 121 ITKSHKVASKLVKAFTDHTVSKSYIAFCVGTSPKWKKINVKSGHGRSKFGVWRVYAAADV 180

Query: 217 GRSLPGGSVVRDMETSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKD 276
           GRSLPGGSVVRDMET FEVLSVNG++T EEL + R+    EEETIV  +KSL+DID+ KD
Sbjct: 181 GRSLPGGSVVRDMETYFEVLSVNGKNTMEELQKFRRG---EEETIVVHTKSLVDIDSHKD 240

Query: 277 EILIRARPRSGRTHQIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPI 336
           EILIRARPRSGRTHQIRLH QYLGIPI GDVKYEGVTEWN KIYDSHELHAESLYFVHP+
Sbjct: 241 EILIRARPRSGRTHQIRLHCQYLGIPIRGDVKYEGVTEWNEKIYDSHELHAESLYFVHPV 300

Query: 337 TGIPLKLQAPLPSWATQALQPQQQEMNSAASLKPMP 367
           TGIPLKLQAPLPSWA+QALQPQQ E+NS  S K MP
Sbjct: 301 TGIPLKLQAPLPSWASQALQPQQHEVNSPQSFKTMP 328

[Close]

BLAST of CmaCh12G010340 vs. NCBI nr
Match: gi|595826525|ref|XP_007205588.1| (hypothetical protein PRUPE_ppa008866mg [Prunus persica])

HSP 1 Score: 449.9 bits (1156), Expect = 4.3e-123
Identity = 225/307 (73.29%), Postives = 260/307 (84.69%), Query Frame = 1


Query: 45  TGENYPVPLSPPLPAISKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYC 104
           T +NYPVPLSPPLP ISK++ELARAM+A SKSSLFSLS  DV++ED+WLI VNK QGVYC
Sbjct: 18  TSQNYPVPLSPPLPPISKHIELARAMSASSKSSLFSLSRDDVVFEDQWLIVVNKPQGVYC 77

Query: 105 ESVLGSVPRFLGDSAMAGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKA 164
           ESVL SVP+ L DSA +G  + ++     ELHLANRLDRDTSG+MVITKSHKVASKLVKA
Sbjct: 78  ESVLESVPQLLCDSAESGTPANQL-----ELHLANRLDRDTSGLMVITKSHKVASKLVKA 137

Query: 165 FTDHKVSKSYIAFCVGSAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDME 224
           FTDHK+SK+YIA C+GSAPKW++I +KSGHGRSKFG WRVYAA+DVGR+LPGGS+VRDME
Sbjct: 138 FTDHKISKTYIARCIGSAPKWERITLKSGHGRSKFGAWRVYAASDVGRTLPGGSMVRDME 197

Query: 225 TSFEVLSVNGQSTKEELPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTH 284
           TSFEVLS+NGQ + +E  E +KDE     TIV Q K++ID D +KDEIL+RARPRSGRTH
Sbjct: 198 TSFEVLSINGQGSLKEPSEFKKDEAN---TIVVQEKAVIDKDTKKDEILVRARPRSGRTH 257

Query: 285 QIRLHSQYLGIPIVGDVKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSW 344
           QIRLH QYLGI I GDVKYEGV EW G+ YD HELHAESL F HPITG+P+  QAP+PSW
Sbjct: 258 QIRLHCQYLGISIRGDVKYEGVYEWKGRTYDGHELHAESLSFEHPITGLPVMFQAPVPSW 316

Query: 345 ATQALQP 352
           A+QALQP
Sbjct: 318 ASQALQP 316

[Close]

BLAST of CmaCh12G010340 vs. NCBI nr
Match: gi|590644653|ref|XP_007031140.1| (Pseudouridine synthase family protein isoform 1 [Theobroma cacao])

HSP 1 Score: 448.0 bits (1151), Expect = 1.6e-122
Identity = 228/351 (64.96%), Postives = 274/351 (78.06%), Query Frame = 1


Query: 9   PLFSIPHKSF--------AKFPKPFRFHRFRPVTNSMADSSKPVTGENYPVPLSPPLPAI 68
           P FS+P +SF        +KFPK   F   +  TN       P + +NYP PLSPPLP+I
Sbjct: 2   PFFSLPQQSFFLCCPKLLSKFPKTLLFLCPKMSTN-------PNSHQNYPTPLSPPLPSI 61

Query: 69  SKNLELARAMAACSKSSLFSLSACDVIYEDEWLIAVNKTQGVYCESVLGSVPRFLGDSAM 128
           SK++ELARAM A SKSSLF LS  D++YEDEWLIAVNK QG+YC+SVL S+PRFL DS +
Sbjct: 62  SKDIELARAMTASSKSSLFELSRSDILYEDEWLIAVNKPQGIYCDSVLASIPRFLSDSTV 121

Query: 129 AGAQSGEIKISLPELHLANRLDRDTSGVMVITKSHKVASKLVKAFTDHKVSKSYIAFCVG 188
           +   S   + S  ELHLANRLDRDTSG+MVITKSHKVA+ LVKAFT+H V K+Y+AFC+G
Sbjct: 122 SDRLSDGNQASPFELHLANRLDRDTSGIMVITKSHKVAAALVKAFTNHMVHKTYVAFCIG 181

Query: 189 SAPKWKKIIVKSGHGRSKFGVWRVYAAADVGRSLPGGSVVRDMETSFEVLSVNGQSTKEE 248
           SAPK ++I +KSGHGRSKFG WRVYAA DVGR LPGGS+VRDM T+FEVLS+NGQ + +E
Sbjct: 182 SAPKSERITIKSGHGRSKFGAWRVYAAGDVGRKLPGGSIVRDMGTTFEVLSINGQQSYKE 241

Query: 249 LPESRKDEEEEEETIVAQSKSLIDIDARKDEILIRARPRSGRTHQIRLHSQYLGIPIVGD 308
             ES KD   EE +++ + K++ID+DA+KDEIL+RA PRSGRTHQIRLH QYLGIPI GD
Sbjct: 242 PSESVKD---EETSVIVEEKAVIDVDAQKDEILVRAFPRSGRTHQIRLHCQYLGIPIRGD 301

Query: 309 VKYEGVTEWNGKIYDSHELHAESLYFVHPITGIPLKLQAPLPSWATQALQP 352
           VKYEGV EW G+ YD+HELHAESL F HP+TG P+ +QAPLP WA+QALQP
Sbjct: 302 VKYEGVYEWKGRRYDAHELHAESLSFQHPVTGFPVMIQAPLPLWASQALQP 342

The following BLAST results are available for this feature:

BLAST of CmaCh12G010340 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PUS1_ARATH	7.1e-94	60.00	RNA pseudouridine synthase 1 OS=Arabidopsis thaliana GN=At1g56345 PE=2 SV=1	[more]
PUS1_ORYSJ	1.7e-92	58.25	RNA pseudouridine synthase 1 OS=Oryza sativa subsp. japonica GN=Os12g0560500 PE=...	[more]
RLUC_RICTY	4.2e-14	28.36	Ribosomal large subunit pseudouridine synthase C OS=Rickettsia typhi (strain ATC...	[more]
RLUD_XANAC	2.1e-13	24.39	Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c...	[more]
RLUD_VIBPA	8.0e-13	26.87	Ribosomal large subunit pseudouridine synthase D OS=Vibrio parahaemolyticus sero...	[more]

BLAST of CmaCh12G010340 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJY6_CUCSA	9.7e-159	79.68	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G033960 PE=4 SV=1	[more]
M5VZ97_PRUPE	3.0e-123	73.29	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008866mg PE=4 SV=1	[more]
A0A061F4Q6_THECC	1.1e-122	64.96	Pseudouridine synthase family protein isoform 1 OS=Theobroma cacao GN=TCM_026756...	[more]
A0A061F426_THECC	1.1e-120	69.23	Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026756...	[more]
A0A067GSR2_CITSI	1.4e-120	66.38	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g018524mg PE=4 SV=1	[more]

BLAST of CmaCh12G010340 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G56345.1	4.0e-95	60.00	Pseudouridine synthase family protein	[more]
AT1G76050.2	4.1e-07	33.02	Pseudouridine synthase family protein	[more]

BLAST of CmaCh12G010340 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700205873\|gb\|KGN60992.1\|	1.4e-158	79.68	hypothetical protein Csa_2G033960 [Cucumis sativus]	[more]
gi\|659070151\|ref\|XP_008453651.1\|	3.5e-149	83.04	PREDICTED: RNA pseudouridine synthase 1 [Cucumis melo]	[more]
gi\|778667027\|ref\|XP_004144814.2\|	6.5e-148	82.44	PREDICTED: RNA pseudouridine synthase 1 [Cucumis sativus]	[more]
gi\|595826525\|ref\|XP_007205588.1\|	4.3e-123	73.29	hypothetical protein PRUPE_ppa008866mg [Prunus persica]	[more]
gi\|590644653\|ref\|XP_007031140.1\|	1.6e-122	64.96	Pseudouridine synthase family protein isoform 1 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006145	PsdUridine_synth_RsuA/RluD
IPR006224	PsdUridine_synth_RluC/D_CS
IPR020103	PsdUridine_synth_cat_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0001522	pseudouridine synthesis
GO:0009451	RNA modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G010340.1	CmaCh12G010340.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	PFAM	PF00849	PseudoU_synth_2	coord: 95..291 score: 1.1
IPR006224	Pseudouridine synthase, RluC/RluD, conserved site	PROSITE	PS01129	PSI_RLU	coord: 138..152 scor
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 84..232 score: 3.68E-43coord: 268..352 score: 3.68
None	No IPR available	PANTHER	PTHR11079	CYTOSINE DEAMINASE	coord: 85..231 score: 1.2E-133coord: 265..363 score: 1.2E-133coord: 58..64 score: 1.2E
None	No IPR available	PANTHER	PTHR11079:SF82	RNA PSEUDOURIDINE SYNTHASE 1	coord: 85..231 score: 1.2E-133coord: 265..363 score: 1.2E-133coord: 58..64 score: 1.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh12G010340	Cucsa.090740	Cucumber (Gy14) v1	cgycmaB0179
CmaCh12G010340	Cla019962	Watermelon (97103) v1	cmawmB170
CmaCh12G010340	Csa2G033960	Cucumber (Chinese Long) v2	cmacuB173
CmaCh12G010340	MELO3C004192	Melon (DHL92) v3.5.1	cmameB172
CmaCh12G010340	ClCG02G014990	Watermelon (Charleston Gray)	cmawcgB156
CmaCh12G010340	CSPI02G04630	Wild cucumber (PI 183967)	cmacpiB174
CmaCh12G010340	CmoCh12G010420	Cucurbita moschata (Rifu)	cmacmoB177
CmaCh12G010340	Lsi11G006250	Bottle gourd (USVL1VR-Ls)	cmalsiB159
CmaCh12G010340	Cp4.1LG07g08590	Cucurbita pepo (Zucchini)	cmacpeB203
CmaCh12G010340	MELO3C004192.2	Melon (DHL92) v3.6.1	cmamedB187
CmaCh12G010340	CsaV3_2G007010	Cucumber (Chinese Long) v3	cmacucB0202
CmaCh12G010340	Cla97C02G040790	Watermelon (97103) v2	cmawmbB175
CmaCh12G010340	Bhi06G001328	Wax gourd	cmawgoB0182
CmaCh12G010340	CsGy2G004770	Cucumber (Gy14) v2	cgybcmaB178
CmaCh12G010340	Carg23265	Silver-seed gourd	carcmaB1221

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh12G010340	Silver-seed gourd	carcmaB0784
CmaCh12G010340	Cucumber (Chinese Long) v3	cmacucB0184
CmaCh12G010340	Cucurbita maxima (Rimu)	cmacmaB190
CmaCh12G010340	Cucurbita moschata (Rifu)	cmacmoB190
CmaCh12G010340	Cucumber (Chinese Long) v2	cmacuB159
CmaCh12G010340	Melon (DHL92) v3.5.1	cmameB151
CmaCh12G010340	Cucurbita pepo (Zucchini)	cmacpeB171
CmaCh12G010340	Bottle gourd (USVL1VR-Ls)	cmalsiB178
CmaCh12G010340	Melon (DHL92) v3.6.1	cmamedB166