Bhi03G000514 (gene) Wax gourd | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Bhi03G000514
Type	gene
Organism	Benincasa hispida (Wax gourd)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	chr3 : 12850190 .. 12850477 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTAAGATGGCTGCTTCATTCAACAAGCTATCTTCTTGGAAACCCGATTGAGGCCCGTGGTGAAGAGAGTAGTTCAAAAGGGAAAAATGGTTATGAAGAAATATGCACTTCTGGGTTTCAAATGCCTCTTCATTACCCTCGCTACAACAAGGCAGATTACCAAAAGATGGAGGAGTGGAAACTGGATCTCCTTCTCAAGGAATATGGCTTGAGTTTTCAAGGCAGTTTGGAGGAGAAGAGGGCCTTTGCGATCGGTGCATTTCTATGGCCTGATCAGTATTGA

mRNA sequence

ATGGCTTTAAGATGGCTGCTTCATTCAACAAGCTATCTTCTTGGAAACCCGATTGAGGCCCGTGGTGAAGAGAGTAGTTCAAAAGGGAAAAATGGTTATGAAGAAATATGCACTTCTGGGTTTCAAATGCCTCTTCATTACCCTCGCTACAACAAGGCAGATTACCAAAAGATGGAGGAGTGGAAACTGGATCTCCTTCTCAAGGAATATGGCTTGAGTTTTCAAGGCAGTTTGGAGGAGAAGAGGGCCTTTGCGATCGGTGCATTTCTATGGCCTGATCAGTATTGA

Coding sequence (CDS)

ATGGCTTTAAGATGGCTGCTTCATTCAACAAGCTATCTTCTTGGAAACCCGATTGAGGCCCGTGGTGAAGAGAGTAGTTCAAAAGGGAAAAATGGTTATGAAGAAATATGCACTTCTGGGTTTCAAATGCCTCTTCATTACCCTCGCTACAACAAGGCAGATTACCAAAAGATGGAGGAGTGGAAACTGGATCTCCTTCTCAAGGAATATGGCTTGAGTTTTCAAGGCAGTTTGGAGGAGAAGAGGGCCTTTGCGATCGGTGCATTTCTATGGCCTGATCAGTATTGA

Protein sequence

MALRWLLHSTSYLLGNPIEARGEESSSKGKNGYEEICTSGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY

Homology

[Close]

BLAST of Bhi03G000514 vs. TAIR10
Match: AT5G55620.1 (unknown protein)

HSP 1 Score: 93.6 bits (231), Expect = 7.1e-20
Identity = 39/56 (69.64%), Postives = 51/56 (91.07%), Query Frame = 0


Query: 39  SGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQ 95
           SGFQ+PLHYP+Y+K+DY+ M++ +LDLLLK+YG SF+GSLE+KR FAI +FLWPDQ
Sbjct: 45  SGFQVPLHYPKYSKSDYEVMDDLRLDLLLKQYGFSFEGSLEDKRVFAIESFLWPDQ 100

[Close]

BLAST of Bhi03G000514 vs. TAIR10
Match: AT3G09950.1 (unknown protein)

HSP 1 Score: 90.1 bits (222), Expect = 7.8e-19
Identity = 47/91 (51.65%), Postives = 61/91 (67.03%), Query Frame = 0


Query: 5  WLLHSTSYLLGNPIEARGEES-SSKGKNGYEEICTSGFQMPLHYPRYNKADYQKMEEWKL 64
          WLL+  ++       A+ +ES  +  KNG  +  +SGF+MPLHYPRY K DY++MEEW+L
Sbjct: 6  WLLNFLNH-------AKNQESVENLDKNGAVKAPSSGFKMPLHYPRYTKEDYEEMEEWRL 65

Query: 65 DLLLKEYGL--SFQGSLEEKRAFAIGAFLWP 93
          DLLL EYGL      +L EKRAFAI  F+WP
Sbjct: 66 DLLLSEYGLLAFHDNTLHEKRAFAIDTFIWP 89

[Close]

BLAST of Bhi03G000514 vs. TAIR10
Match: AT5G41761.1 (unknown protein)

HSP 1 Score: 85.5 bits (210), Expect = 1.9e-17
Identity = 42/88 (47.73%), Postives = 59/88 (67.05%), Query Frame = 0


Query: 7  LHSTSYLLGNPIEARGEESSSKGKNGYEEICTSGFQMPLHYPRYNKADYQKMEEWKLDLL 66
          +H+ S+  G   E +  E+++K  +   +  +S FQ+PLHYP+Y K+DY+KM EW+LD L
Sbjct: 14 IHNRSHQDG---EDQRNETATKINHDKPQNQSSSFQIPLHYPKYTKSDYEKMPEWQLDRL 73

Query: 67 LKEYGLSFQGSLEEKRAFAIGAFLWPDQ 95
          L+EYGL   G   EKR FAIGAFLW  +
Sbjct: 74 LREYGLPVIGDSYEKRKFAIGAFLWSSE 98

[Close]

BLAST of Bhi03G000514 vs. TAIR10
Match: AT3G55570.1 (unknown protein)

HSP 1 Score: 83.6 bits (205), Expect = 7.3e-17
Identity = 40/63 (63.49%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 29 GKNGYEEICTSGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGA 88
          GKNG   +    F+MPLHYPRY+K DYQ M EWKLD +L +YGLS  G L  KR FAIGA
Sbjct: 24 GKNGEGSV----FRMPLHYPRYSKEDYQDMPEWKLDRVLADYGLSTYGDLAHKRDFAIGA 82

Query: 89 FLW 92
          FLW
Sbjct: 84 FLW 82

[Close]

BLAST of Bhi03G000514 vs. TAIR10
Match: AT3G11405.1 (unknown protein)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-09
Identity = 31/54 (57.41%), Postives = 35/54 (64.81%), Query Frame = 0


Query: 39  SGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQ-GSLEEKRAFAIGAFLW 92
           S FQMPL YP Y K  Y  M E +LD LLK YGL    G+L  K+ FA+GAFLW
Sbjct: 55  SSFQMPLQYPNYAKEQYDIMSEEELDRLLKLYGLPTDIGNLSCKKEFAVGAFLW 108

[Close]

BLAST of Bhi03G000514 vs. TrEMBL
Match: tr|A0A0A0KMW8|A0A0A0KMW8_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511080 PE=4 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 6.3e-39
Identity = 81/96 (84.38%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 1  MALRWLLHSTSYLLGNPIEAR--GEESSSKGKNGYEEICTSGFQMPLHYPRYNKADYQKM 60
          M LR LLHS SYLLGNP EA   GEE SSKGK GYEE+C SGFQMPLHYPRY K+DYQKM
Sbjct: 1  MDLRGLLHSVSYLLGNPNEAHAYGEERSSKGKKGYEELCNSGFQMPLHYPRYKKSDYQKM 60

Query: 61 EEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQ 95
          EEWKLDLLLKEYGLSF+GSLEEKRAFA+GAFLWPDQ
Sbjct: 61 EEWKLDLLLKEYGLSFEGSLEEKRAFAMGAFLWPDQ 96

[Close]

BLAST of Bhi03G000514 vs. TrEMBL
Match: tr|A0A2N9EZP3|A0A2N9EZP3_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8195 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 5.5e-27
Identity = 62/100 (62.00%), Postives = 78/100 (78.00%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLGNPIEA--RGEESSSKGKNG---YEEICTSGFQMPLHYPRYNKADY 60
           MAL WL+HS  ++LG P +   +    S K  NG    +E+  SGFQMPLHYPRYNKADY
Sbjct: 1   MALSWLIHSACHVLGTPKDTNIQCHVKSLKVPNGGLPSKEMNPSGFQMPLHYPRYNKADY 60

Query: 61  QKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
           +KMEEWK+DLLLK+YGL+F+G+L+EKRA+A+GAFLWP QY
Sbjct: 61  EKMEEWKVDLLLKQYGLNFKGNLDEKRAYAMGAFLWPGQY 100

[Close]

BLAST of Bhi03G000514 vs. TrEMBL
Match: tr|A0A067GFM8|A0A067GFM8_CITSI (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g045350mg PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 7.2e-27
Identity = 62/118 (52.54%), Postives = 79/118 (66.95%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLG--------------------NPIEARGEESSSKGKNGYE---EIC 60
           MALRW+LH+  ++LG                    + ++  G  S SK  +G     E C
Sbjct: 1   MALRWVLHTACHVLGHQNDNKIECNGVVGYQNDHHHQVQVNGVSSDSKVSDGLSQSVEAC 60

Query: 61  TSGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
            SGFQ+PLHYPRY+KADY+KME+WKLD+LL+EYGL FQG+ +EKRAFA+GAFLWPDQY
Sbjct: 61  ASGFQLPLHYPRYSKADYEKMEDWKLDMLLREYGLCFQGTPDEKRAFAMGAFLWPDQY 118

[Close]

BLAST of Bhi03G000514 vs. TrEMBL
Match: tr|I1L021|I1L021_SOYBN (Uncharacterized protein OS=Glycine max OX=3847 GN=102666531 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-26
Identity = 61/103 (59.22%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLGNPIEARGEESSSKG----KNGYEEI----CTSGFQMPLHYPRYNK 60
           MALRW+L+S  ++LG P+    EE+  K      N + E+     +SGFQMPLHYPRY K
Sbjct: 1   MALRWVLYSACHVLGYPMSNTEEEAECKKGLCLANNFNEVDQYYPSSGFQMPLHYPRYTK 60

Query: 61  ADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
            DY+ MEEW +DLLLK+YGLSF+G+L EKRAFA+GAFLWPDQY
Sbjct: 61  QDYESMEEWTVDLLLKQYGLSFKGTLHEKRAFAMGAFLWPDQY 103

[Close]

BLAST of Bhi03G000514 vs. TrEMBL
Match: tr|B9HD46|B9HD46_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G017700v3 PE=4 SV=2)

HSP 1 Score: 126.3 bits (316), Expect = 3.6e-26
Identity = 64/109 (58.72%), Postives = 79/109 (72.48%), Query Frame = 0


Query: 2   ALRWLLHSTSYLLGNPIEARGEESSSKG-------------KNGYEEI--CTSGFQMPLH 61
           ALRWL+HS  ++LG P E    +S +K              KN   E+  CT GFQMPLH
Sbjct: 3   ALRWLVHSACHVLGYPNEDSNMQSRNKVVGCPNEHANGVMIKNSRLEMNPCT-GFQMPLH 62

Query: 62  YPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
           YPRY KADY+KMEEWK+D+LL+EYG+SF+G+LEEKRA+A+GAFLWPDQY
Sbjct: 63  YPRYAKADYEKMEEWKVDMLLREYGISFKGNLEEKRAYAMGAFLWPDQY 110

[Close]

BLAST of Bhi03G000514 vs. NCBI nr
Match: XP_011658113.1 (PREDICTED: uncharacterized protein LOC105435946 [Cucumis sativus] >KGN49041.1 hypothetical protein Csa_6G511080 [Cucumis sativus])

HSP 1 Score: 168.7 bits (426), Expect = 9.5e-39
Identity = 81/96 (84.38%), Postives = 85/96 (88.54%), Query Frame = 0


Query: 1  MALRWLLHSTSYLLGNPIEAR--GEESSSKGKNGYEEICTSGFQMPLHYPRYNKADYQKM 60
          M LR LLHS SYLLGNP EA   GEE SSKGK GYEE+C SGFQMPLHYPRY K+DYQKM
Sbjct: 1  MDLRGLLHSVSYLLGNPNEAHAYGEERSSKGKKGYEELCNSGFQMPLHYPRYKKSDYQKM 60

Query: 61 EEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQ 95
          EEWKLDLLLKEYGLSF+GSLEEKRAFA+GAFLWPDQ
Sbjct: 61 EEWKLDLLLKEYGLSFEGSLEEKRAFAMGAFLWPDQ 96

[Close]

BLAST of Bhi03G000514 vs. NCBI nr
Match: XP_022132797.1 (uncharacterized protein LOC111005569 [Momordica charantia])

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-31
Identity = 69/104 (66.35%), Postives = 79/104 (75.96%), Query Frame = 0


Query: 1   MALRWLLHSTSYLL-------GNPIEARGEESSSKGKNGYEEIC--TSGFQMPLHYPRYN 60
           MAL+WLLHS  YLL          ++   EE   KGK G EEIC   SGFQMPLHYPRYN
Sbjct: 3   MALKWLLHSACYLLNEVHACANGGVKTSDEERIPKGKKGCEEICNSASGFQMPLHYPRYN 62

Query: 61  KADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
           KADYQKMEEW++DLLL +YG+ F+GSLEEKRAFA+GAFLWPDQ+
Sbjct: 63  KADYQKMEEWEVDLLLNQYGMGFEGSLEEKRAFAMGAFLWPDQF 106

[Close]

BLAST of Bhi03G000514 vs. NCBI nr
Match: XP_024047458.1 (uncharacterized protein LOC18050630 [Citrus clementina])

HSP 1 Score: 131.3 bits (329), Expect = 1.7e-27
Identity = 64/118 (54.24%), Postives = 80/118 (67.80%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLG--------------------NPIEARGEESSSKGKNGYE---EIC 60
           MALRW+LH+  ++LG                    + ++A G  S SK  +G     E C
Sbjct: 1   MALRWVLHTACHVLGHQNDNKIECKGVVGYQNDHHHQVQANGVSSDSKVSDGLSQSVEAC 60

Query: 61  TSGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
            SGFQ+PLHYPRY+KADY+KME+WKLD+LL+EYGL FQG+ EEKRAFA+GAFLWPDQY
Sbjct: 61  ASGFQLPLHYPRYSKADYEKMEDWKLDMLLREYGLCFQGTPEEKRAFAMGAFLWPDQY 118

[Close]

BLAST of Bhi03G000514 vs. NCBI nr
Match: KDO78523.1 (hypothetical protein CISIN_1g045350mg [Citrus sinensis])

HSP 1 Score: 128.6 bits (322), Expect = 1.1e-26
Identity = 62/118 (52.54%), Postives = 79/118 (66.95%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLG--------------------NPIEARGEESSSKGKNGYE---EIC 60
           MALRW+LH+  ++LG                    + ++  G  S SK  +G     E C
Sbjct: 1   MALRWVLHTACHVLGHQNDNKIECNGVVGYQNDHHHQVQVNGVSSDSKVSDGLSQSVEAC 60

Query: 61  TSGFQMPLHYPRYNKADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
            SGFQ+PLHYPRY+KADY+KME+WKLD+LL+EYGL FQG+ +EKRAFA+GAFLWPDQY
Sbjct: 61  ASGFQLPLHYPRYSKADYEKMEDWKLDMLLREYGLCFQGTPDEKRAFAMGAFLWPDQY 118

[Close]

BLAST of Bhi03G000514 vs. NCBI nr
Match: XP_006586775.1 (uncharacterized protein LOC102666531 [Glycine max] >KHN40806.1 hypothetical protein glysoja_015173 [Glycine soja] >KRH36583.1 hypothetical protein GLYMA_09G012700 [Glycine max])

HSP 1 Score: 127.1 bits (318), Expect = 3.2e-26
Identity = 61/103 (59.22%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MALRWLLHSTSYLLGNPIEARGEESSSKG----KNGYEEI----CTSGFQMPLHYPRYNK 60
           MALRW+L+S  ++LG P+    EE+  K      N + E+     +SGFQMPLHYPRY K
Sbjct: 1   MALRWVLYSACHVLGYPMSNTEEEAECKKGLCLANNFNEVDQYYPSSGFQMPLHYPRYTK 60

Query: 61  ADYQKMEEWKLDLLLKEYGLSFQGSLEEKRAFAIGAFLWPDQY 96
            DY+ MEEW +DLLLK+YGLSF+G+L EKRAFA+GAFLWPDQY
Sbjct: 61  QDYESMEEWTVDLLLKQYGLSFKGTLHEKRAFAMGAFLWPDQY 103

The following BLAST results are available for this feature:

BLAST of Bhi03G000514 vs. Swiss-Prot


Match Name	E-value	Identity	Description

BLAST of Bhi03G000514 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G55620.1	7.1e-20	69.64	unknown protein	[more]
AT3G09950.1	7.8e-19	51.65	unknown protein	[more]
AT5G41761.1	1.9e-17	47.73	unknown protein	[more]
AT3G55570.1	7.3e-17	63.49	unknown protein	[more]
AT3G11405.1	1.1e-09	57.41	unknown protein	[more]

BLAST of Bhi03G000514 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KMW8\|A0A0A0KMW8_CUCSA	6.3e-39	84.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511080 PE=4 SV=1	[more]
tr\|A0A2N9EZP3\|A0A2N9EZP3_FAGSY	5.5e-27	62.00	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8195 PE=4 SV=1	[more]
tr\|A0A067GFM8\|A0A067GFM8_CITSI	7.2e-27	52.54	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g045350mg PE=4 SV=1	[more]
tr\|I1L021\|I1L021_SOYBN	2.1e-26	59.22	Uncharacterized protein OS=Glycine max OX=3847 GN=102666531 PE=4 SV=1	[more]
tr\|B9HD46\|B9HD46_POPTR	3.6e-26	58.72	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_006G017700v3 PE=...	[more]

BLAST of Bhi03G000514 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011658113.1	9.5e-39	84.38	PREDICTED: uncharacterized protein LOC105435946 [Cucumis sativus] >KGN49041.1 hy...	[more]
XP_022132797.1	3.3e-31	66.35	uncharacterized protein LOC111005569 [Momordica charantia]	[more]
XP_024047458.1	1.7e-27	54.24	uncharacterized protein LOC18050630 [Citrus clementina]	[more]
KDO78523.1	1.1e-26	52.54	hypothetical protein CISIN_1g045350mg [Citrus sinensis]	[more]
XP_006586775.1	3.2e-26	59.22	uncharacterized protein LOC102666531 [Glycine max] >KHN40806.1 hypothetical prot...	[more]

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0008643	carbohydrate transport
biological_process	GO:0015992	proton transport
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0000139	Golgi membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0005351	sugar:proton symporter activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi03M000514	Bhi03M000514	mRNA

InterPro

Analysis Name: InterPro Annotations of wax gourd
Date Performed: 2019-11-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33513:SF2	SUBFAMILY NOT NAMED	coord: 1..95
None	No IPR available	PANTHER	PTHR33513	FAMILY NOT NAMED	coord: 1..95

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Bhi03G000514	Csa6G511080	Cucumber (Chinese Long) v2	cuwgoB628
Bhi03G000514	MELO3C007449	Melon (DHL92) v3.5.1	mewgoB635
Bhi03G000514	ClCG01G021310	Watermelon (Charleston Gray)	wcgwgoB227
Bhi03G000514	Cucsa.044650	Cucumber (Gy14) v1	cgywgoB026
Bhi03G000514	CmaCh03G005860	Cucurbita maxima (Rimu)	cmawgoB0788
Bhi03G000514	CmoCh03G006110	Cucurbita moschata (Rifu)	cmowgoB0781
Bhi03G000514	CmoCh07G003480	Cucurbita moschata (Rifu)	cmowgoB1051
Bhi03G000514	Lsi01G004930	Bottle gourd (USVL1VR-Ls)	lsiwgoB168
Bhi03G000514	Cp4.1LG19g08000	Cucurbita pepo (Zucchini)	cpewgoB0607
Bhi03G000514	MELO3C007449.2	Melon (DHL92) v3.6.1	medwgoB641
Bhi03G000514	CsaV3_6G048330	Cucumber (Chinese Long) v3	cucwgoB648

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None