Bhi10G001329 (gene) Wax gourd

BLAST of Bhi10G001329 vs. TAIR10
Match: AT5G09760.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 710.3 bits (1832), Expect = 9.8e-205
Identity = 347/504 (68.85%), Postives = 408/504 (80.95%), Query Frame = 0

Query: 65  ACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNL 124
           AC ATR+PDQC SSLS    +PP+P P+QI+ SA+S S   L TAQS +K I+DSS  NL
Sbjct: 48  ACNATRYPDQCVSSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNL 107

Query: 125 NRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 184
           NRT AA  CL++L  S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT 
Sbjct: 108 NRTNAANTCLQLLTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTS 167

Query: 185 LVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGL---- 244
            V +TM+FLD LI ++SNALSM+V+YD +G++ A+WT P TERDGFWE  G G GL    
Sbjct: 168 QVGETMSFLDGLIHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPST 227

Query: 245 GFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVP 304
           G + GFPS +  D TVCKDG  G Y TVQ+AV+AAPE+   R+FVI I EGVYEE V VP
Sbjct: 228 GLNLGFPSGLKEDVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVP 287

Query: 305 LEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAH 364
            EKKNVVF+GDGMGKTVITGSLN G PG++TYNTATVGV+GDGFMA  LT QNTAGPDAH
Sbjct: 288 FEKKNVVFIGDGMGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAH 347

Query: 365 QAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEI 424
           QAVAFRSDSD S+I++CEF+GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEI
Sbjct: 348 QAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEI 407

Query: 425 LVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLG 484
           L+ PRQ+ PEKGE NA+TAQGR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLG
Sbjct: 408 LIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLG 467

Query: 485 RPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQ 544
           RPWK+YSRTVFI CNLE+L+TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQ
Sbjct: 468 RPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQ 527

Query: 545 IPAQHVFSYSVQNFIQGDEWIPSS 564
           IP +HV  YSV NFIQ DEW   S
Sbjct: 528 IPDEHVHVYSVANFIQADEWASMS 550

BLAST of Bhi10G001329 vs. TAIR10
Match: AT5G64640.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 648.3 bits (1671), Expect = 4.6e-186
Identity = 318/502 (63.35%), Postives = 389/502 (77.49%), Query Frame = 0

Query: 65  ACKATRFPDQCGSSLSH-SSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNL 124
           AC ATRFPD C +SLS    +PP+P P+QI+ SA+S+S + L + QS ++ ILDSSA N 
Sbjct: 96  ACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDSSAGNR 155

Query: 125 NRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 184
           NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS LK  NDT+
Sbjct: 156 NRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTVNDTK 215

Query: 185 LVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG----GGGSGL 244
            V  T+ F + L+ L+ NALSM++++D +G+D  +W  P TERDGFWE      G G+G 
Sbjct: 216 QVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGSGTGT 275

Query: 245 GFSGGFPSKMAADATVCKDGAGGC-YGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRV 304
             S GFPS +  D TVCK+G   C Y TVQEAVD+AP+     +FVI I+EGVYEE VRV
Sbjct: 276 EASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRV 335

Query: 305 PLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDA 364
           P EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATVGVLGDGFMA  LTI+NTAG DA
Sbjct: 336 PFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADA 395

Query: 365 HQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 424
           HQAVAFRSDSD SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA++FQDC+
Sbjct: 396 HQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCD 455

Query: 425 ILVRPRQLKPEK-GENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNF 484
           IL+  +  K E+ G NNAITA GR D +QSTGFVF NC INGT+EYM  + + P+ HKNF
Sbjct: 456 ILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNF 515

Query: 485 LGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS 544
           LGRPWKE+SRTVF++CNLESL++P GWMPW+GDFALKTL+YGE++N+G GS  S RV WS
Sbjct: 516 LGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWS 575

Query: 545 SQIPAQHVFSYSVQNFIQGDEW 560
           S+IP +HV  YSV NFIQ DEW
Sbjct: 576 SEIPEKHVDVYSVANFIQADEW 597

BLAST of Bhi10G001329 vs. TAIR10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 307.0 bits (785), Expect = 2.5e-83
Identity = 197/503 (39.17%), Postives = 280/503 (55.67%), Query Frame = 0

Query: 66  CKATRFPDQCGSSLSH-SSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNLN 125
           C  T  P  C   L+H S+  P  S  + L+ ++ L  D    A++     L    ++  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPKCRDTR 93

Query: 126 RTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQL 185
              A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T +
Sbjct: 94  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 153

Query: 186 VDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTA-AWTPPRTERDGFWEGGGGGSGLGFSG 245
           V          + L SN +S L+   +  N     +TPP  E+DGF      G       
Sbjct: 154 V----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLLQS 213

Query: 246 GFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 305
             P     +A V KDG+G  + T++EA+DAA   + + RFVI++K+GVY E   + + KK
Sbjct: 214 STPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIRKK 273

Query: 306 NVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVA 365
           NV+  GDG+GKT+ITGS +VG  G +T+N+ATV  +GDGF+A G+T +NTAG    QAVA
Sbjct: 274 NVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 333

Query: 366 FRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRP 425
            RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C I  R 
Sbjct: 334 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFAR- 393

Query: 426 RQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWK 485
              +P + + N ITAQGR+DP Q+TG +  N  +    +   +  S     K +LGRPW+
Sbjct: 394 ---RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPWR 453

Query: 486 EYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSS---QI 545
           +YSRTVF+  +L+SL+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W       
Sbjct: 454 QYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLG 504

Query: 546 PAQHVFSYSVQNFIQGDEWIPSS 564
            A     ++V  F+ G  WIPSS
Sbjct: 514 SASEASKFTVGTFLAGGSWIPSS 504

BLAST of Bhi10G001329 vs. TAIR10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 305.1 bits (780), Expect = 9.5e-83
Identity = 191/532 (35.90%), Postives = 284/532 (53.38%), Query Frame = 0

Query: 66  CKATRFPDQCGSSLSHSSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNLNR 125
           C  T F D C +SL  +S P +  P+ +++    ++   ++  +S+ K   D  AK  ++
Sbjct: 55  CAPTDFKDTCVNSLMGAS-PDSDDPVDLIKLGFKVTIKSIN--ESLEKASGDIKAK-ADK 114

Query: 126 TTAAKNCLEVLRN------------SDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 185
              AK   E+                D+  S+    +     +D R WLS S+ +   C 
Sbjct: 115 NPEAKGAFELCEKLMIDAIDDLKKCMDHGFSVDQIEV---FVEDLRVWLSGSIAFQQTCM 174

Query: 186 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAY-------DIYGNDTAAWTPPR--- 245
            +        + D    F  S   LSSN+L+M+          ++ G   A     R   
Sbjct: 175 DSFGEIKSNLMQDMLKIFKTSR-ELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLL 234

Query: 246 TERDGF--WEGGGGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAAR 305
           +  D    W G      +   GG P  + A+A V +DG G  + T+ +A++A P+     
Sbjct: 235 STEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKV- 294

Query: 306 RFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGD 365
            F+IHIKEG+Y+E V V  +  +V F+GDG  KT+ITGSLN G   + T+ TAT+ + GD
Sbjct: 295 PFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGD 354

Query: 366 GFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQG 425
            F A  + I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G
Sbjct: 355 HFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSG 414

Query: 426 NVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQ 485
            VDFIFG++  I Q+C+I+VR    KP KG+   +TAQGR++  +STG V   C I G  
Sbjct: 415 TVDFIFGDAKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDP 474

Query: 486 EYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEF 545
            Y+ +      V+K +LGRPWKE+SRT+ +   ++ ++ PAGW+PWSGDFALKTL+Y E 
Sbjct: 475 AYIPM----KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEH 534

Query: 546 ENSGVGSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKM 572
            N+G GS  ++RV W    ++  Q    Y+   F++GD WIP +Q     K+
Sbjct: 535 MNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTAKV 568

BLAST of Bhi10G001329 vs. TAIR10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 298.5 bits (763), Expect = 8.9e-81
Identity = 169/422 (40.05%), Postives = 246/422 (58.29%), Query Frame = 0

Query: 156 KDARAWLSASLLYHYDCWSAL-----KYANDTQLVDKTMAFLDSL-IGLSSNALSMLVAY 215
           +D R WLS S+ Y   C         K + D Q + KT   L S  + + +N  ++L  +
Sbjct: 156 EDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEF 215

Query: 216 DIYG--NDTAAWTPP-RTERDGF--WEGGGGGSGLGFSGGFPSKMAADATVCKDGAGGCY 275
           ++ G   D   +     +  DG   W G      +   GG    + A+  V  DG+G  Y
Sbjct: 216 NVTGVTGDLGKYARKLLSAEDGIPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-Y 275

Query: 276 GTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVG 335
            T+ EA++A P+ A  + FVI+IK+GVY E V V  +  +V F+GDG  KT ITGSLN  
Sbjct: 276 KTINEALNAVPK-ANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYY 335

Query: 336 QPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDT 395
              + TY TATV + GD F A  +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDT
Sbjct: 336 IGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDT 395

Query: 396 LYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDP 455
           LY HS RQF++ C + G VDFIFG+   + Q+C I+VR    KP K ++  ITAQGR+D 
Sbjct: 396 LYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDK 455

Query: 456 AQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGW 515
            +STG V QNC I G   Y+ +      ++K +LGRPWKE+SRT+ +   ++ ++ PAGW
Sbjct: 456 RESTGLVLQNCHITGEPAYIPV----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGW 515

Query: 516 MPWSGDFALKTLFYGEFENSGVGSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPS 565
           +PW+GDFAL TL+Y E+EN+G GS  ++RV W    ++  +    ++   F++G+ WIP 
Sbjct: 516 LPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPP 563

BLAST of Bhi10G001329 vs. Swiss-Prot
Match: sp|Q9LXD9|PME51_ARATH (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3702 GN=PME51 PE=2 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.8e-203
Identity = 347/504 (68.85%), Postives = 408/504 (80.95%), Query Frame = 0

Query: 65  ACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNL 124
           AC ATR+PDQC SSLS    +PP+P P+QI+ SA+S S   L TAQS +K I+DSS  NL
Sbjct: 48  ACNATRYPDQCVSSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNL 107

Query: 125 NRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 184
           NRT AA  CL++L  S++R   T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT 
Sbjct: 108 NRTNAANTCLQLLTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTS 167

Query: 185 LVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGL---- 244
            V +TM+FLD LI ++SNALSM+V+YD +G++ A+WT P TERDGFWE  G G GL    
Sbjct: 168 QVGETMSFLDGLIHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPST 227

Query: 245 GFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVP 304
           G + GFPS +  D TVCKDG  G Y TVQ+AV+AAPE+   R+FVI I EGVYEE V VP
Sbjct: 228 GLNLGFPSGLKEDVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVP 287

Query: 305 LEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAH 364
            EKKNVVF+GDGMGKTVITGSLN G PG++TYNTATVGV+GDGFMA  LT QNTAGPDAH
Sbjct: 288 FEKKNVVFIGDGMGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAH 347

Query: 365 QAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEI 424
           QAVAFRSDSD S+I++CEF+GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEI
Sbjct: 348 QAVAFRSDSDFSLIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEI 407

Query: 425 LVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLG 484
           L+ PRQ+ PEKGE NA+TAQGR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLG
Sbjct: 408 LIAPRQINPEKGEKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLG 467

Query: 485 RPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQ 544
           RPWK+YSRTVFI CNLE+L+TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQ
Sbjct: 468 RPWKDYSRTVFIGCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQ 527

Query: 545 IPAQHVFSYSVQNFIQGDEWIPSS 564
           IP +HV  YSV NFIQ DEW   S
Sbjct: 528 IPDEHVHVYSVANFIQADEWASMS 550

BLAST of Bhi10G001329 vs. Swiss-Prot
Match: sp|Q8L7Q7|PME64_ARATH (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3702 GN=PME64 PE=2 SV=2)

HSP 1 Score: 648.3 bits (1671), Expect = 8.2e-185
Identity = 318/502 (63.35%), Postives = 389/502 (77.49%), Query Frame = 0

Query: 65  ACKATRFPDQCGSSLSH-SSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNL 124
           AC ATRFPD C +SLS    +PP+P P+QI+ SA+S+S + L + QS ++ ILDSSA N 
Sbjct: 96  ACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDSSAGNR 155

Query: 125 NRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 184
           NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS LK  NDT+
Sbjct: 156 NRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTVNDTK 215

Query: 185 LVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG----GGGSGL 244
            V  T+ F + L+ L+ NALSM++++D +G+D  +W  P TERDGFWE      G G+G 
Sbjct: 216 QVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGSGTGT 275

Query: 245 GFSGGFPSKMAADATVCKDGAGGC-YGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRV 304
             S GFPS +  D TVCK+G   C Y TVQEAVD+AP+     +FVI I+EGVYEE VRV
Sbjct: 276 EASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEETVRV 335

Query: 305 PLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDA 364
           P EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATVGVLGDGFMA  LTI+NTAG DA
Sbjct: 336 PFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTAGADA 395

Query: 365 HQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 424
           HQAVAFRSDSD SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA++FQDC+
Sbjct: 396 HQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVFQDCD 455

Query: 425 ILVRPRQLKPEK-GENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNF 484
           IL+  +  K E+ G NNAITA GR D +QSTGFVF NC INGT+EYM  + + P+ HKNF
Sbjct: 456 ILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEGHKNF 515

Query: 485 LGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS 544
           LGRPWKE+SRTVF++CNLESL++P GWMPW+GDFALKTL+YGE++N+G GS  S RV WS
Sbjct: 516 LGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSRVPWS 575

Query: 545 SQIPAQHVFSYSVQNFIQGDEW 560
           S+IP +HV  YSV NFIQ DEW
Sbjct: 576 SEIPEKHVDVYSVANFIQADEW 597

BLAST of Bhi10G001329 vs. Swiss-Prot
Match: sp|O04887|PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 3.7e-84
Identity = 196/473 (41.44%), Postives = 274/473 (57.93%), Query Frame = 0

Query: 97  AVSLSSDGLSTAQSMVKQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTK 156
           ++ L+ +  +TAQS     L S  +N     A ++C E+   +  +++ T+++       
Sbjct: 64  SLQLALERATTAQSRT-YTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 123

Query: 157 DARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDT 216
           D + WLS +L     C ++L          + +   + ++ L SN ++ L++  +  N  
Sbjct: 124 DKQTWLSTALTNLETCRASL----------EDLGVPEYVLPLLSNNVTKLISNTLSLNKV 183

Query: 217 AAWTPPRTERDGF--WEGGGGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAA 276
               P  + +DGF  W   G    L       +   A+  V +DG+G    T+QEAV AA
Sbjct: 184 PYNEP--SYKDGFPTWVKPGDRKLL------QTTPRANIVVAQDGSGN-VKTIQEAV-AA 243

Query: 277 PENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTA 336
              A   R+VI+IK G Y E + V L  KN++F+GDG+GKT+ITGS +VG  G +T+ +A
Sbjct: 244 ASRAGGSRYVIYIKAGTYNENIEVKL--KNIMFVGDGIGKTIITGSKSVG-GGATTFKSA 303

Query: 337 TVGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFY 396
           TV V+GD F+A  +TI+NTAGP+ HQAVA RS SDLSV   C F G QDTLY HS RQFY
Sbjct: 304 TVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFY 363

Query: 397 KSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQN 456
           + C I G VDFIFGN+A + Q+C I  R    KP     N +TAQGRTDP QSTG +  N
Sbjct: 364 RECDIYGTVDFIFGNAAVVLQNCNIFAR----KP-PNRTNTLTAQGRTDPNQSTGIIIHN 423

Query: 457 CLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALK 516
           C +    +   +  S     K FLGRPWK+YSRTV+I   L+SL+ PAGWM WSGDFAL 
Sbjct: 424 CRVTAASDLKPVQSSV----KTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALN 483

Query: 517 TLFYGEFENSGVGSKTSERVGWSS----QIPAQHVFSYSVQNFIQGDEWIPSS 564
           TL+Y E+ N+G GS T+ RV W        P+Q V  ++V NFI G+ W+P++
Sbjct: 484 TLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQ-VSQFTVGNFIAGNSWLPAT 502

BLAST of Bhi10G001329 vs. Swiss-Prot
Match: sp|O22149|PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 307.0 bits (785), Expect = 4.5e-82
Identity = 197/503 (39.17%), Postives = 280/503 (55.67%), Query Frame = 0

Query: 66  CKATRFPDQCGSSLSH-SSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNLN 125
           C  T  P  C   L+H S+  P  S  + L+ ++ L  D    A++     L    ++  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPKCRDTR 93

Query: 126 RTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQL 185
              A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +       T +
Sbjct: 94  EKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDI 153

Query: 186 VDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTA-AWTPPRTERDGFWEGGGGGSGLGFSG 245
           V          + L SN +S L+   +  N     +TPP  E+DGF      G       
Sbjct: 154 V----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDRKLLQS 213

Query: 246 GFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 305
             P     +A V KDG+G  + T++EA+DAA   + + RFVI++K+GVY E   + + KK
Sbjct: 214 STPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NLEIRKK 273

Query: 306 NVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVA 365
           NV+  GDG+GKT+ITGS +VG  G +T+N+ATV  +GDGF+A G+T +NTAG    QAVA
Sbjct: 274 NVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVA 333

Query: 366 FRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRP 425
            RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C I  R 
Sbjct: 334 LRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFAR- 393

Query: 426 RQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWK 485
              +P + + N ITAQGR+DP Q+TG +  N  +    +   +  S     K +LGRPW+
Sbjct: 394 ---RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPWR 453

Query: 486 EYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSS---QI 545
           +YSRTVF+  +L+SL+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W       
Sbjct: 454 QYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLG 504

Query: 546 PAQHVFSYSVQNFIQGDEWIPSS 564
            A     ++V  F+ G  WIPSS
Sbjct: 514 SASEASKFTVGTFLAGGSWIPSS 504

BLAST of Bhi10G001329 vs. Swiss-Prot
Match: sp|Q8GXA1|PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 305.1 bits (780), Expect = 1.7e-81
Identity = 191/532 (35.90%), Postives = 284/532 (53.38%), Query Frame = 0

Query: 66  CKATRFPDQCGSSLSHSSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNLNR 125
           C  T F D C +SL  +S P +  P+ +++    ++   ++  +S+ K   D  AK  ++
Sbjct: 55  CAPTDFKDTCVNSLMGAS-PDSDDPVDLIKLGFKVTIKSIN--ESLEKASGDIKAK-ADK 114

Query: 126 TTAAKNCLEVLRN------------SDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 185
              AK   E+                D+  S+    +     +D R WLS S+ +   C 
Sbjct: 115 NPEAKGAFELCEKLMIDAIDDLKKCMDHGFSVDQIEV---FVEDLRVWLSGSIAFQQTCM 174

Query: 186 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAY-------DIYGNDTAAWTPPR--- 245
            +        + D    F  S   LSSN+L+M+          ++ G   A     R   
Sbjct: 175 DSFGEIKSNLMQDMLKIFKTSR-ELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLL 234

Query: 246 TERDGF--WEGGGGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAAR 305
           +  D    W G      +   GG P  + A+A V +DG G  + T+ +A++A P+     
Sbjct: 235 STEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKV- 294

Query: 306 RFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGD 365
            F+IHIKEG+Y+E V V  +  +V F+GDG  KT+ITGSLN G   + T+ TAT+ + GD
Sbjct: 295 PFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGD 354

Query: 366 GFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQG 425
            F A  + I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G
Sbjct: 355 HFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSG 414

Query: 426 NVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQ 485
            VDFIFG++  I Q+C+I+VR    KP KG+   +TAQGR++  +STG V   C I G  
Sbjct: 415 TVDFIFGDAKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDP 474

Query: 486 EYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEF 545
            Y+ +      V+K +LGRPWKE+SRT+ +   ++ ++ PAGW+PWSGDFALKTL+Y E 
Sbjct: 475 AYIPM----KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEH 534

Query: 546 ENSGVGSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKM 572
            N+G GS  ++RV W    ++  Q    Y+   F++GD WIP +Q     K+
Sbjct: 535 MNTGPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTAKV 568

BLAST of Bhi10G001329 vs. TrEMBL
Match: tr|A0A1S3CD05|A0A1S3CD05_CUCME (LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1)

HSP 1 Score: 1028.1 bits (2657), Expect = 7.7e-297
Identity = 539/588 (91.67%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 3   PRKTHFPISKSTLLFLS-------XXXXXXXXXXXXXXXXXXXXXXXRHRHHHLSTHFTN 62
           PRK  FPISKSTLLFLS       XXXXXXXXXXXXXXXXXXXXXXX        +HFTN
Sbjct: 4   PRKPTFPISKSTLLFLSMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSHFTN 63

Query: 63  PPSIDGILHACKATRFPDQCGSSLSHSSL-PPNPSPLQILQSAVSLSSDGLSTAQSMVKQ 122
            PS D ILHACK+TRFPDQCGSSLSHSS  PPNPSPLQILQSA++LSSDGL+TAQS+VKQ
Sbjct: 64  TPS-DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLVKQ 123

Query: 123 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 182
           ILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDCWS
Sbjct: 124 ILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWS 183

Query: 183 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG 242
           ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG
Sbjct: 184 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG 243

Query: 243 GGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 302
           GGSGLGFSGGFP+K+ ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEE
Sbjct: 244 GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEE 303

Query: 303 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTA 362
           IVRVPLEKKNVVFLGDGMGKTVITGSLN GQPG+STYNTAT+GVLGDGFMASGLTIQNTA
Sbjct: 304 IVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTA 363

Query: 363 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 422
           GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF
Sbjct: 364 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 423

Query: 423 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 482
           QDCEIL+RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH
Sbjct: 424 QDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 483

Query: 483 KNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERV 542
           KNFLGRPWKEYSRTVFIHCNLE+LV+P+GWMPWSGDFALKTLFYGEFENSGVGSKTSERV
Sbjct: 484 KNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERV 543

Query: 543 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSKDVKLD 583
           GWSSQIPAQHVFSYSVQNFIQGDEWIPSS++SN+ KMLNKKSKDVKLD
Sbjct: 544 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 590

BLAST of Bhi10G001329 vs. TrEMBL
Match: tr|A0A0A0LQ80|A0A0A0LQ80_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 1.2e-294
Identity = 534/586 (91.13%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 3   PRKTHFPISKSTLLFLS------XXXXXXXXXXXXXXXXXXXXXXXRHRHHHLSTHFTNP 62
           PRK  FP SKSTLLFLS      XXXXXXXXXXXXXXXXXXXXXXX      L +HFTN 
Sbjct: 4   PRKPTFPFSKSTLLFLSMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPSHFTNY 63

Query: 63  PSIDGILHACKATRFPDQCGSSLSHSSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQIL 122
           PS D ILHACK+TRFPDQCGSSLSHSSLPPNPSPLQILQSA++LSSDGL+TAQS+VKQIL
Sbjct: 64  PS-DQILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQIL 123

Query: 123 DSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSAL 182
           DSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHYDCWSAL
Sbjct: 124 DSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSAL 183

Query: 183 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGG 242
           KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE GGGG
Sbjct: 184 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GGGG 243

Query: 243 SGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIV 302
           SGLGFSGGFP+K+ ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEEIV
Sbjct: 244 SGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIV 303

Query: 303 RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGP 362
           RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTAT+GV+GDGFMASGLTIQNTAGP
Sbjct: 304 RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGP 363

Query: 363 DAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD 422
           DAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD
Sbjct: 364 DAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD 423

Query: 423 CEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKN 482
           CEILVRPRQLKPEKGENNA+TA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VHKN
Sbjct: 424 CEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKN 483

Query: 483 FLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGW 542
           FLGRPWKEYSRTVFIHCNLE+LV+P+GWMPWSGDFALKTLFYGEFENSG GSKTSERVGW
Sbjct: 484 FLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGW 543

Query: 543 SSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSKDVKLD 583
           SSQIPAQHVFSYSV+NFIQGDEWIPSS++S+E KMLNK+SKDVKLD
Sbjct: 544 SSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

BLAST of Bhi10G001329 vs. TrEMBL
Match: tr|A0A2P5E9H9|A0A2P5E9H9_9ROSA (Pectinesterase OS=Trema orientalis OX=63057 GN=TorRG33x02_219930 PE=3 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 8.7e-240
Identity = 404/517 (78.14%), Postives = 462/517 (89.36%), Query Frame = 0

Query: 62  ILHACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSA 121
           I  ACKATRF + C ++LS S  + P+P P+Q++Q+A+++S++ L TA SMV+ IL SS 
Sbjct: 48  IQQACKATRFQEPCEAALSQSGRVGPDPGPVQVIQAAIAVSAENLRTADSMVRSILGSSG 107

Query: 122 KNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYAN 181
            NLNR+TAAKNCLEVL NSDYR +L N+AL RGRTKDARAW+SA+LLY YDCWSALKYAN
Sbjct: 108 GNLNRSTAAKNCLEVLSNSDYRTTLANEALPRGRTKDARAWMSAALLYQYDCWSALKYAN 167

Query: 182 DTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLG 241
           DT+LV++TM+FLDSLIGLSSNALSM+ +YD +GNDT+ W PPRTERDGFW G G GS LG
Sbjct: 168 DTRLVNETMSFLDSLIGLSSNALSMVASYDAFGNDTSTWAPPRTERDGFWAGSGSGSELG 227

Query: 242 FSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPL 301
             GGFPSK+ ADATVCKDG  GCY TVQEAV+AAP+N   RRFVIHIKEGVYEE VRVPL
Sbjct: 228 SRGGFPSKLTADATVCKDGGDGCYKTVQEAVNAAPDN-GVRRFVIHIKEGVYEETVRVPL 287

Query: 302 EKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQ 361
           EKKNVVFLGDGMGKT+ITGSLNVGQPG+STYNTATVGVLGDGFMA+GLTIQNTAGPDAHQ
Sbjct: 288 EKKNVVFLGDGMGKTIITGSLNVGQPGISTYNTATVGVLGDGFMANGLTIQNTAGPDAHQ 347

Query: 362 AVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL 421
           AVAFRSDSDLS+I++CEF+GNQDTLYAHSLRQFYKSC+I+GNVDFIFGNSASIFQDC+IL
Sbjct: 348 AVAFRSDSDLSIIENCEFLGNQDTLYAHSLRQFYKSCRIEGNVDFIFGNSASIFQDCKIL 407

Query: 422 VRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGR 481
           VRPRQLKPEKGENNA+TA GRTDPAQSTGFVFQNCLINGT+EYM LYY KPKVHKNFLGR
Sbjct: 408 VRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTEEYMKLYYDKPKVHKNFLGR 467

Query: 482 PWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQI 541
           PWKEYSRTVFIHCNLE+L+TP GW+PWS DFALKTLFYGEFENSG GS  S+RV WSSQI
Sbjct: 468 PWKEYSRTVFIHCNLEALITPQGWLPWSQDFALKTLFYGEFENSGAGSDLSQRVSWSSQI 527

Query: 542 PAQHVFSYSVQNFIQGDEWIPSSQTSNEP-KMLNKKS 577
           PA+HV  YSVQ+FIQGDEWIP+ + ++ P K++++K+
Sbjct: 528 PAEHVSIYSVQSFIQGDEWIPTKEPASFPRKVIHRKN 563

BLAST of Bhi10G001329 vs. TrEMBL
Match: tr|A0A2P5DGQ3|A0A2P5DGQ3_PARAD (Pectinesterase OS=Parasponia andersonii OX=3476 GN=PanWU01x14_065130 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 2.5e-239
Identity = 405/517 (78.34%), Postives = 461/517 (89.17%), Query Frame = 0

Query: 62  ILHACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSA 121
           I  ACKATRF + C ++LS S  + P+PSP+QI+Q A+++S++ L TA SMV+ IL SS 
Sbjct: 48  IQQACKATRFQEPCEAALSRSGRVGPDPSPVQIIQVAIAVSAENLRTADSMVRSILGSSG 107

Query: 122 KNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYAN 181
            NLNR+TAAKNCLEVL NSDYR +L N+AL RGRTKDARAW+SA+LLY YDCWSALKYAN
Sbjct: 108 GNLNRSTAAKNCLEVLSNSDYRTTLANEALPRGRTKDARAWMSAALLYQYDCWSALKYAN 167

Query: 182 DTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLG 241
           DT+LV++TM+FLDSLIGLSSNALSM+ +YD +GNDT+ W PPRTERDGFW G G GS LG
Sbjct: 168 DTRLVNETMSFLDSLIGLSSNALSMVASYDTFGNDTSTWAPPRTERDGFWAGSGSGSELG 227

Query: 242 FSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPL 301
             GGFPSK+ ADATVCKDG  GCY TVQEAV+AAP+N   RRFVIHIKEGVYEE VRVPL
Sbjct: 228 SRGGFPSKLTADATVCKDGGDGCYKTVQEAVNAAPDN-GVRRFVIHIKEGVYEETVRVPL 287

Query: 302 EKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQ 361
           EKKNVVFLGDGMGKT+ITGSLNVGQPG+STYNTATVGVLGDGFMA+GLTIQNTAGPDAHQ
Sbjct: 288 EKKNVVFLGDGMGKTIITGSLNVGQPGISTYNTATVGVLGDGFMANGLTIQNTAGPDAHQ 347

Query: 362 AVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL 421
           AVAFRSDSDLS+I++CEF+GNQDTLYAHSLRQFYKSC+I+GNVDFIFGNSASIFQDCEIL
Sbjct: 348 AVAFRSDSDLSIIENCEFLGNQDTLYAHSLRQFYKSCRIEGNVDFIFGNSASIFQDCEIL 407

Query: 422 VRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGR 481
           VRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCLINGT+EYM LYY KPKVHKNFLGR
Sbjct: 408 VRPRQLKPEKGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMKLYYDKPKVHKNFLGR 467

Query: 482 PWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQI 541
           PWKEYSRTVFIHCNLE+L+T  GW+PWS DFALKTLFYGEFENSG GS  S+RV WSSQI
Sbjct: 468 PWKEYSRTVFIHCNLEALITLQGWLPWSQDFALKTLFYGEFENSGAGSNLSQRVSWSSQI 527

Query: 542 PAQHVFSYSVQNFIQGDEWIPSSQTSNEP-KMLNKKS 577
           PA+H+  YSVQ+FIQGDEWIP+ + ++ P K+++KK+
Sbjct: 528 PAEHISIYSVQSFIQGDEWIPTKEPASFPLKVIHKKN 563

BLAST of Bhi10G001329 vs. TrEMBL
Match: tr|A0A2N9FFB5|A0A2N9FFB5_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17439 PE=3 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 4.9e-235
Identity = 404/512 (78.91%), Postives = 452/512 (88.28%), Query Frame = 0

Query: 56  PPSIDGILHACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQ 115
           PP I     ACKATRFPD C +SLS S  +PPNP+PLQ++ SA+SLSS  L TAQSMVK 
Sbjct: 38  PPEIH---QACKATRFPDPCETSLSQSGHVPPNPTPLQLINSALSLSSTNLRTAQSMVKS 97

Query: 116 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISL-TNDALRRGRTKDARAWLSASLLYHYDCW 175
           ILDSS  N NRTTA KNC+EVL NS YR+S+ T+DAL RG+ KDARAW+SA+LLY YDCW
Sbjct: 98  ILDSSVGNQNRTTATKNCVEVLNNSQYRMSISTDDALPRGKLKDARAWMSAALLYQYDCW 157

Query: 176 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG 235
           SALKYANDT LV++TM+FLDSL  L+SNAL+M+V+YD++GNDTA+W PPRTERDGFWE G
Sbjct: 158 SALKYANDTHLVNETMSFLDSLTQLTSNALNMVVSYDVFGNDTASWVPPRTERDGFWE-G 217

Query: 236 GGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENA-AARRFVIHIKEGVY 295
           G     GF GGFPSK+ AD TVCKDG+GGCY TVQEAV+AAP NA   +RFVI IKEGVY
Sbjct: 218 GXXXXXGFKGGFPSKLTADVTVCKDGSGGCYKTVQEAVNAAPNNADGGKRFVISIKEGVY 277

Query: 296 EEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQN 355
           EE VR+PLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTATV VLGDGFMASGLTIQN
Sbjct: 278 EETVRIPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVAVLGDGFMASGLTIQN 337

Query: 356 TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS 415
           TAGPDAHQAVAFRS SDLSVI++CEFIGNQDTLYA SLRQFYKSC+IQGNVDFIFGNSAS
Sbjct: 338 TAGPDAHQAVAFRSASDLSVIENCEFIGNQDTLYAQSLRQFYKSCRIQGNVDFIFGNSAS 397

Query: 416 IFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK 475
            FQDCEILVRPRQ+ PEKGENNA+TA GRTDPAQSTGFVFQNCLINGTQEYM+LY SKP+
Sbjct: 398 FFQDCEILVRPRQVNPEKGENNALTAHGRTDPAQSTGFVFQNCLINGTQEYMALYNSKPQ 457

Query: 476 VHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSE 535
           VHKN+LGRPWKEYSRTV+IHCN E+LVTP GWMPWS DFALKTL+YGEF NSG+GS  S+
Sbjct: 458 VHKNYLGRPWKEYSRTVYIHCNFEALVTPQGWMPWSDDFALKTLYYGEFGNSGLGSTLSQ 517

Query: 536 RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQ 565
           RV WSS+IPA+H+++YSV+NFIQGDEWIPSSQ
Sbjct: 518 RVTWSSKIPAEHIYTYSVENFIQGDEWIPSSQ 545

BLAST of Bhi10G001329 vs. NCBI nr
Match: XP_008460902.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 1028.1 bits (2657), Expect = 1.2e-296
Identity = 539/588 (91.67%), Postives = 560/588 (95.24%), Query Frame = 0

Query: 3   PRKTHFPISKSTLLFLS-------XXXXXXXXXXXXXXXXXXXXXXXRHRHHHLSTHFTN 62
           PRK  FPISKSTLLFLS       XXXXXXXXXXXXXXXXXXXXXXX        +HFTN
Sbjct: 4   PRKPTFPISKSTLLFLSMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPSHFTN 63

Query: 63  PPSIDGILHACKATRFPDQCGSSLSHSSL-PPNPSPLQILQSAVSLSSDGLSTAQSMVKQ 122
            PS D ILHACK+TRFPDQCGSSLSHSS  PPNPSPLQILQSA++LSSDGL+TAQS+VKQ
Sbjct: 64  TPS-DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLVKQ 123

Query: 123 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 182
           ILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDCWS
Sbjct: 124 ILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWS 183

Query: 183 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG 242
           ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG
Sbjct: 184 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG 243

Query: 243 GGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 302
           GGSGLGFSGGFP+K+ ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEE
Sbjct: 244 GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEE 303

Query: 303 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTA 362
           IVRVPLEKKNVVFLGDGMGKTVITGSLN GQPG+STYNTAT+GVLGDGFMASGLTIQNTA
Sbjct: 304 IVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTA 363

Query: 363 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 422
           GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF
Sbjct: 364 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 423

Query: 423 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 482
           QDCEIL+RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH
Sbjct: 424 QDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 483

Query: 483 KNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERV 542
           KNFLGRPWKEYSRTVFIHCNLE+LV+P+GWMPWSGDFALKTLFYGEFENSGVGSKTSERV
Sbjct: 484 KNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERV 543

Query: 543 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSKDVKLD 583
           GWSSQIPAQHVFSYSVQNFIQGDEWIPSS++SN+ KMLNKKSKDVKLD
Sbjct: 544 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 590

BLAST of Bhi10G001329 vs. NCBI nr
Match: XP_004147375.2 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.1 hypothetical protein Csa_2G302130 [Cucumis sativus])

HSP 1 Score: 1020.8 bits (2638), Expect = 1.9e-294
Identity = 534/586 (91.13%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 3   PRKTHFPISKSTLLFLS------XXXXXXXXXXXXXXXXXXXXXXXRHRHHHLSTHFTNP 62
           PRK  FP SKSTLLFLS      XXXXXXXXXXXXXXXXXXXXXXX      L +HFTN 
Sbjct: 4   PRKPTFPFSKSTLLFLSMAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLPSHFTNY 63

Query: 63  PSIDGILHACKATRFPDQCGSSLSHSSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQIL 122
           PS D ILHACK+TRFPDQCGSSLSHSSLPPNPSPLQILQSA++LSSDGL+TAQS+VKQIL
Sbjct: 64  PS-DQILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQIL 123

Query: 123 DSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSAL 182
           DSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHYDCWSAL
Sbjct: 124 DSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSAL 183

Query: 183 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGG 242
           KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE GGGG
Sbjct: 184 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GGGG 243

Query: 243 SGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIV 302
           SGLGFSGGFP+K+ ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEEIV
Sbjct: 244 SGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIV 303

Query: 303 RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGP 362
           RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTAT+GV+GDGFMASGLTIQNTAGP
Sbjct: 304 RVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGP 363

Query: 363 DAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD 422
           DAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD
Sbjct: 364 DAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQD 423

Query: 423 CEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKN 482
           CEILVRPRQLKPEKGENNA+TA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VHKN
Sbjct: 424 CEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKN 483

Query: 483 FLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGW 542
           FLGRPWKEYSRTVFIHCNLE+LV+P+GWMPWSGDFALKTLFYGEFENSG GSKTSERVGW
Sbjct: 484 FLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGW 543

Query: 543 SSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSKDVKLD 583
           SSQIPAQHVFSYSV+NFIQGDEWIPSS++S+E KMLNK+SKDVKLD
Sbjct: 544 SSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

BLAST of Bhi10G001329 vs. NCBI nr
Match: XP_022932006.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita moschata])

HSP 1 Score: 948.0 bits (2449), Expect = 1.5e-272
Identity = 460/524 (87.79%), Postives = 496/524 (94.66%), Query Frame = 0

Query: 51  THFTNPPSIDGILHACKATRFPDQCGSSLSHS-SLPPNPSPLQILQSAVSLSSDGLSTAQ 110
           +HFTN PS +    ACKATRFPDQCGSSLSHS SLPPNP+ LQI+QSA+++SSDGL++A+
Sbjct: 61  SHFTNSPSSEETFRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAK 120

Query: 111 SMVKQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYH 170
           SMVKQILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYH
Sbjct: 121 SMVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYH 180

Query: 171 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGF 230
           YDCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWTPPRTERDGF
Sbjct: 181 YDCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGF 240

Query: 231 WEGGGGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKE 290
           WEG  GGSGLGFSGGFPSK+ A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKE
Sbjct: 241 WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKE 300

Query: 291 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLT 350
           GVY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTATVGV+GDGFMASGLT
Sbjct: 301 GVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLT 360

Query: 351 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 410
           IQNTAGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGN
Sbjct: 361 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 420

Query: 411 SASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYS 470
           SAS+FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCL+NGTQEYM+LYYS
Sbjct: 421 SASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLVNGTQEYMALYYS 480

Query: 471 KPKVHKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSK 530
           KPKVHKNFLGRPWKEYSRTVFIHCNLE+LV+PAGWMPW+GDFALKTL+YGEFENSG GSK
Sbjct: 481 KPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSK 540

Query: 531 TSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLN 574
           TSERV WSSQIPAQHV+SYSVQNFIQGDEWIPSSQ+S E K +N
Sbjct: 541 TSERVEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

BLAST of Bhi10G001329 vs. NCBI nr
Match: XP_022971621.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita maxima])

HSP 1 Score: 943.0 bits (2436), Expect = 4.9e-271
Identity = 481/579 (83.07%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 2   VPRKTHFPI------SKSTLLFLSXXXXXXXXXXXXXXXXXXXXXXXRHRHHHLSTHFTN 61
           +PR T FP       +KST LFLS             XXXXXXXXXX     HL +HFTN
Sbjct: 5   LPRTTPFPFLFQFTTAKSTFLFLS-MAALSFLSFLCLXXXXXXXXXXXXXXXHLPSHFTN 64

Query: 62  PPSIDGILHACKATRFPDQCGSSLSHS-SLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQ 121
            PS +  L ACKATRFP+QCGSSLSHS SLPPNP+ LQI+QSA+++SSDGL++A+SMVK+
Sbjct: 65  FPSSEETLRACKATRFPNQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKK 124

Query: 122 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 181
           ILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHYDCWS
Sbjct: 125 ILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWS 184

Query: 182 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGG 241
           ALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWTPPRTERDGFWEG  
Sbjct: 185 ALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFWEGSS 244

Query: 242 GGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 301
           GGSGLGFSGGFPSK+ A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKEGVY+E
Sbjct: 245 GGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDE 304

Query: 302 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTA 361
           IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYN+ATVGV+GDGFMASGLTIQNTA
Sbjct: 305 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVVGDGFMASGLTIQNTA 364

Query: 362 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 421
           GPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+F
Sbjct: 365 GPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVF 424

Query: 422 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 481
           QDCEILV PRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCLINGTQEYM+LYYSKPKVH
Sbjct: 425 QDCEILVCPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVH 484

Query: 482 KNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERV 541
           KNFLGRPWKEYSRTVFIHCNLE+LV+PAGWMPW+GDFALKTL+YGEFENSG GS TSERV
Sbjct: 485 KNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSITSERV 544

Query: 542 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLN 574
            WSSQIPAQHV+SYSVQNFIQGDEWIPSSQ+S E K +N
Sbjct: 545 EWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 582

BLAST of Bhi10G001329 vs. NCBI nr
Match: XP_023512339.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 936.8 bits (2420), Expect = 3.5e-269
Identity = 456/520 (87.69%), Postives = 492/520 (94.62%), Query Frame = 0

Query: 55  NPPSIDGILHACKATRFPDQCGSSLSHS-SLPPNPSPLQILQSAVSLSSDGLSTAQSMVK 114
           N PS +  L ACKATRFPDQCGSSLSHS SLPPNP+ LQI+QSA+++SSDGL++A+SMVK
Sbjct: 63  NSPSSEETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVK 122

Query: 115 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 174
           QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHYDCW
Sbjct: 123 QILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCW 182

Query: 175 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG 234
           SALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWT PRTERDGFWEG 
Sbjct: 183 SALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGS 242

Query: 235 GGGSGLGFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYE 294
            GGSGLGFSGGFPSK+ A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKEGVY+
Sbjct: 243 SGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYD 302

Query: 295 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNT 354
           EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTATVGV+GDGFMASGLTIQNT
Sbjct: 303 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNT 362

Query: 355 AGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASI 414
           AGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+
Sbjct: 363 AGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASV 422

Query: 415 FQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKV 474
           FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCLINGTQEYM+LYYSKPKV
Sbjct: 423 FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKV 482

Query: 475 HKNFLGRPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSER 534
           HKNFLGRPWKEYSRTVFIHCNLE+LV+PAGWMPW+GDFALKTL+YGEFENSG GSKTSER
Sbjct: 483 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 542

Query: 535 VGWSSQIPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLN 574
           V WSS+IPAQHV+SYS+QNFIQGDEWIPSSQ+S E K +N
Sbjct: 543 VEWSSKIPAQHVYSYSIQNFIQGDEWIPSSQSSEESKTIN 582