Cp4.1LG16g00820 (gene) Cucurbita pepo (Zucchini)

BLAST of Cp4.1LG16g00820 vs. Swiss-Prot
Match: PME51_ARATH (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana GN=PME51 PE=2 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 2.7e-204
Identity = 361/552 (65.40%), Postives = 432/552 (78.26%), Query Frame = 1

Query: 29  MAALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFTN-SPSSEETLR---ACKATRFPDQCG 88
           M+++  L F SLF F S SS+  HHHHH+S   +  +PSS    +   AC ATR+PDQC 
Sbjct: 1   MSSILILLF-SLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCV 60

Query: 89  SSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNCLEV 148
           SSLS    +PP+P  +QII SAI+ S   L +A+S +K I+DSS  + NRT AA  CL++
Sbjct: 61  SSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQL 120

Query: 149 LRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFLDSL 208
           L +SE+R   T+ A+ RG+ KDARAWMSA+L+Y YD WSALKY NDT  V +TM+FLD L
Sbjct: 121 LTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGL 180

Query: 209 IGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGL----GFSGGFPSKVDA 268
           I +TSNALSM+ +YD  G++ A+WT P TERDGFWE +  G GL    G + GFPS +  
Sbjct: 181 IHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKE 240

Query: 269 SATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDG 328
             TVCKDG  G YKTVQ+AVNAAP++   ++FVI I EGVY+E V VP EKKNVVF+GDG
Sbjct: 241 DVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDG 300

Query: 329 MGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLS 388
           MGKTVITGSLN G PGI+TYNTATVGVVGDGFMA  LT QNTAGPDAHQAVAFRSDSD S
Sbjct: 301 MGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFS 360

Query: 389 VIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKG 448
           +I++CEFLGNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA+VFQDCEIL+ PRQ+ PEKG
Sbjct: 361 LIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKG 420

Query: 449 ENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFI 508
           E NAVTA GR DP+Q+TGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI
Sbjct: 421 EKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFI 480

Query: 509 HCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSYSIQ 568
            CNLEAL++P GW+PW+GDFALKTLYYGE +N+G GS  S+RV WSS+IP +HV+ YS+ 
Sbjct: 481 GCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVA 540

Query: 569 NFIQGDEWIPSS 573
           NFIQ DEW   S
Sbjct: 541 NFIQADEWASMS 550

BLAST of Cp4.1LG16g00820 vs. Swiss-Prot
Match: PME64_ARATH (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana GN=PME64 PE=2 SV=2)

HSP 1 Score: 647.5 bits (1669), Expect = 1.4e-184
Identity = 332/566 (58.66%), Postives = 414/566 (73.14%), Query Frame = 1

Query: 20  AKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHHHH--HHSFHFTNSPSSE--------- 79
           +++  L +  A+   L  +SL  + ++S +  +HH+  H +    + P  E         
Sbjct: 32  SRNGILIIIAASCILLLLISLLIYATVSKSSRNHHNPSHQTPTSDDHPPPETPPSPPPIA 91

Query: 80  ETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSS 139
           +   AC ATRFPD C +SLS    +PP+P  +QII SAI+VS + L S +S ++ ILDSS
Sbjct: 92  QIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDSS 151

Query: 140 TDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYA 199
             + NRT  A  CLE+L +S++R   T+ AV  G  KDARAWMSA+L Y +DCWS LK  
Sbjct: 152 AGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTV 211

Query: 200 NDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWE--GSSGGS 259
           NDT+ V  T+ F + L+ LT NALSM+ ++D  G+D  +W  P TERDGFWE  G S GS
Sbjct: 212 NDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGS 271

Query: 260 GLGFSG--GFPSKVDASATVCKDGAGGC-YKTVQEAVNAAPDNAMGKRFVIHIKEGVYDE 319
           G G     GFPS +    TVCK+G   C YKTVQEAV++APD     +FVI I+EGVY+E
Sbjct: 272 GTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEE 331

Query: 320 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTA 379
            VRVP EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATVGV+GDGFMA  LTI+NTA
Sbjct: 332 TVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTA 391

Query: 380 GPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVF 439
           G DAHQAVAFRSDSD SV+++CEFLGNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA+VF
Sbjct: 392 GADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVF 451

Query: 440 QDCEILVRPRQLKPEK-GENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKV 499
           QDC+IL+  +  K E+ G NNA+TAHGR D +Q+TGFVF NC INGT+EYM  + + P+ 
Sbjct: 452 QDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEG 511

Query: 500 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 559
           HKNFLGRPWKE+SRTVF++CNLE+L+SP GWMPW GDFALKTLYYGE++N+G GS  S R
Sbjct: 512 HKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSR 571

Query: 560 VEWSSKIPAQHVYSYSIQNFIQGDEW 569
           V WSS+IP +HV  YS+ NFIQ DEW
Sbjct: 572 VPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of Cp4.1LG16g00820 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.3e-83
Identity = 193/532 (36.28%), Postives = 287/532 (53.95%), Query Frame = 1

Query: 74  CKATRFPDQCGSSLSHSPSLPPNP---------TTLQIIQSAIAVSSDGLAS-------- 133
           C  T + + C +SL  +      P          T++ I+ +I  +S  L +        
Sbjct: 59  CAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDT 118

Query: 134 --AKSMVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSAS 193
             A  + +++++ +TD        K CL+   F  + I    D V     +D R W+S S
Sbjct: 119 KGALELCEKLMNDATDD------LKKCLD--NFDGFSIPQIEDFV-----EDLRVWLSGS 178

Query: 194 LLYHYDCWSALKYAN-----DTQSVNKTMAFLDSL-IGLTSNALSMLAAYDISGNDTAAW 253
           + Y   C    +  N     D Q + KT   L S  + + +N  ++L  ++++G      
Sbjct: 179 IAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG 238

Query: 254 TLPR---TERDGF--WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAA 313
              R   +  DG   W G +    +   GG    V A+  V  DG+G  YKT+ EA+NA 
Sbjct: 239 KYARKLLSAEDGIPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-YKTINEALNAV 298

Query: 314 PDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTA 373
           P  A  K FVI+IK+GVY+E V V  +  +V F+GDG  KT ITGSLN     + TY TA
Sbjct: 299 P-KANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTA 358

Query: 374 TVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 433
           TV + GD F A  +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDTLY HS RQF+
Sbjct: 359 TVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFF 418

Query: 434 KSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 493
           + C + G VDFIFG+   V Q+C I+VR    KP K ++  +TA GR+D  ++TG V QN
Sbjct: 419 RDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRESTGLVLQN 478

Query: 494 CLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 553
           C I G   Y+ +      ++K +LGRPWKE+SRT+ +   ++ ++ PAGW+PW GDFAL 
Sbjct: 479 CHITGEPAYIPV----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALN 538

Query: 554 TLYYGEFENSGTGSKTSERVEWS--SKIPAQHVYSYSIQNFIQGDEWIPSSQ 574
           TLYY E+EN+G GS  ++RV+W    K+  +    ++   F++G+ WIP ++
Sbjct: 539 TLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNR 563

BLAST of Cp4.1LG16g00820 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-82
Identity = 211/549 (38.43%), Postives = 300/549 (54.64%), Query Frame = 1

Query: 31  ALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFTNSPSSEETLRACKATRFPDQCGSSLSHS 90
           AL  L  +SL  F SLS        H SF +    S EE    C  T  P  C   L+  
Sbjct: 2   ALRILITVSLVLF-SLS--------HTSFGY----SPEEVKSWCGKTPNPQPCEYFLTQK 61

Query: 91  PSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNCLEVLRFSEY 150
             +          + ++ ++ +   +A+S     L S   +     A ++C E+   +  
Sbjct: 62  TDVTSIKQDTDFYKISLQLALERATTAQSRT-YTLGSKCRNEREKAAWEDCRELYELTVL 121

Query: 151 RISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFLDSLIGLTSN 210
           +++ T+++       D + W+S +L     C ++L+           +   + ++ L SN
Sbjct: 122 KLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLE----------DLGVPEYVLPLLSN 181

Query: 211 ALSMLAAYDISGNDTAAWTLPRTE---RDGFWEGSSGGSGLGFSGGFPSKVDASATVCKD 270
            ++ L +  +S N      +P  E   +DGF      G          +   A+  V +D
Sbjct: 182 NVTKLISNTLSLNK-----VPYNEPSYKDGFPTWVKPGDRKLLQ----TTPRANIVVAQD 241

Query: 271 GAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDGMGKTVIT 330
           G+G   KT+QEAV AA   A G R+VI+IK G Y+E + V L  KN++F+GDG+GKT+IT
Sbjct: 242 GSGNV-KTIQEAV-AAASRAGGSRYVIYIKAGTYNENIEVKL--KNIMFVGDGIGKTIIT 301

Query: 331 GSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEF 390
           GS +VG  G +T+ +ATV VVGD F+A  +TI+NTAGP+ HQAVA RS SDLSV   C F
Sbjct: 302 GSKSVGG-GATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSF 361

Query: 391 LGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTA 450
            G QDTLY HS RQFY+ C I G VDFIFGN+A V Q+C I  R    KP     N +TA
Sbjct: 362 EGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFAR----KP-PNRTNTLTA 421

Query: 451 HGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEAL 510
            GRTDP Q+TG +  NC +    +   +  S     K FLGRPWK+YSRTV+I   L++L
Sbjct: 422 QGRTDPNQSTGIIIHNCRVTAASDLKPVQSSV----KTFLGRPWKQYSRTVYIKTFLDSL 481

Query: 511 VSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSS----KIPAQHVYSYSIQNFI 570
           ++PAGWM W+GDFAL TLYY E+ N+G GS T+ RV+W        P+Q V  +++ NFI
Sbjct: 482 INPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQ-VSQFTVGNFI 502

Query: 571 QGDEWIPSS 573
            G+ W+P++
Sbjct: 542 AGNSWLPAT 502

BLAST of Cp4.1LG16g00820 vs. Swiss-Prot
Match: PME17_ARATH (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana GN=PME17 PE=2 SV=2)

HSP 1 Score: 305.8 bits (782), Expect = 9.9e-82
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 1

Query: 74  CKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPN 133
           C  T  P  C   L+H+ +  P  +  + ++ ++ +  D    AK+    +     D+  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTRE 93

Query: 134 RTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQS 193
           +  A ++C+++   +  +I+ T D   +    DA+ W+S +L     C +       T  
Sbjct: 94  KA-AWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVT-- 153

Query: 194 VNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLGFSGG 253
                   D ++ L SN +S L    ++ N       P  E+DGF      G        
Sbjct: 154 --------DIVLPLMSNNVSNLLCNTLAINKVPFNYTP-PEKDGFPSWVKPGDRKLLQSS 213

Query: 254 FPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKN 313
            P     +A V KDG+G  +KT++EA++AA  +    RFVI++K+GVY E   + + KKN
Sbjct: 214 TPKD---NAVVAKDGSGN-FKTIKEAIDAASGSG---RFVIYVKQGVYSE--NLEIRKKN 273

Query: 314 VVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAF 373
           V+  GDG+GKT+ITGS +VG  G +T+N+ATV  VGDGF+A G+T +NTAG    QAVA 
Sbjct: 274 VMLRGDGIGKTIITGSKSVGG-GTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVAL 333

Query: 374 RSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPR 433
           RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A+V Q+C I  R  
Sbjct: 334 RSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFAR-- 393

Query: 434 QLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKE 493
             +P + + N +TA GR+DP Q TG +  N  +    +   +  S     K +LGRPW++
Sbjct: 394 --RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPWRQ 453

Query: 494 YSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSS---KIP 553
           YSRTVF+  +L++L+ P GW+ W G+FALKTL+Y EF+N+G G+ TS RV W        
Sbjct: 454 YSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGS 504

Query: 554 AQHVYSYSIQNFIQGDEWIPSS 573
           A     +++  F+ G  WIPSS
Sbjct: 514 ASEASKFTVGTFLAGGSWIPSS 504

BLAST of Cp4.1LG16g00820 vs. TrEMBL
Match: A0A0A0LQ80_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G302130 PE=4 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 7.9e-280
Identity = 491/584 (84.08%), Postives = 533/584 (91.27%), Query Frame = 1

Query: 6   PRTTPFPFPFQFATAKSTFLFLSMAALSFLSFLSLFFF-----LSLSSAVHHHHHHHSF- 65
           PR   FPF      +KST LFLSMA+LSFLSFLSLFFF     LS SSA HHHH H    
Sbjct: 4   PRKPTFPF------SKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLP 63

Query: 66  -HFTNSPSSEETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAK 125
            HFTN PS ++ L ACK+TRFPDQCGSSLSHS SLPPNP+ LQI+QSAIA+SSDGL +A+
Sbjct: 64  SHFTNYPS-DQILHACKSTRFPDQCGSSLSHS-SLPPNPSPLQILQSAIALSSDGLTTAQ 123

Query: 126 SMVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYH 185
           S+VKQILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+  GRTKDARAW+SASLLYH
Sbjct: 124 SLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYH 183

Query: 186 YDCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGF 245
           YDCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWT PRTERDGF
Sbjct: 184 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGF 243

Query: 246 WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKE 305
           WEG  GGSGLGFSGGFP+KV A ATVCKDG+GGCY ++Q+AV+AAP+NA  +RFVIHIKE
Sbjct: 244 WEGG-GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKE 303

Query: 306 GVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLT 365
           GVY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTAT+GVVGDGFMASGLT
Sbjct: 304 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLT 363

Query: 366 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 425
           IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN
Sbjct: 364 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 423

Query: 426 SASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYS 485
           SAS+FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQ+TGFVFQNCLINGTQEYM+LYYS
Sbjct: 424 SASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYS 483

Query: 486 KPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSK 545
           KP+VHKNFLGRPWKEYSRTVFIHCNLEALVSP+GWMPW+GDFALKTL+YGEFENSG GSK
Sbjct: 484 KPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSK 543

Query: 546 TSERVEWSSKIPAQHVYSYSIQNFIQGDEWIPSSQSSEESKTIN 583
           TSERV WSS+IPAQHV+SYS++NFIQGDEWIPSS+SS+E K +N
Sbjct: 544 TSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLN 578

BLAST of Cp4.1LG16g00820 vs. TrEMBL
Match: B9RU20_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1460660 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.3e-234
Identity = 409/552 (74.09%), Postives = 464/552 (84.06%), Query Frame = 1

Query: 33  SFLSF--LSLFFFLSLSSAVHHHHHHH-------SFHFTNSPSSEETLRACKATRFPDQC 92
           SFLSF  LSL+ FLS +S    HH +        S   +  P   E L+ACKATRFPD C
Sbjct: 3   SFLSFSLLSLYLFLSSTSVTSRHHTYRPKLPPPSSLSVSTPP---EILQACKATRFPDTC 62

Query: 93  GSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNCLE 152
            SSL+ S S+P NPT LQIIQS++++S+  L +A+SMVK ILDSS  + NRT AA+NCLE
Sbjct: 63  ASSLTSSDSVPKNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGNINRTNAARNCLE 122

Query: 153 VLRFSEYRISLTND-AVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFLD 212
           VL  SEYRIS T D ++ RGRTKDARAWMSA+LLY YDCWSALKYANDT   NKTM+FLD
Sbjct: 123 VLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYANDTSDTNKTMSFLD 182

Query: 213 SLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLG--FSGGFPSKVDA 272
           SL+ LTSN+LSM+ +YDI GNDT  WT P+TERDGFWEGS  GSG G  F G FPSK+ A
Sbjct: 183 SLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGLVFRGVFPSKLTA 242

Query: 273 SATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDG 332
             TVCKDG+ GCYKTVQEAVN APDN MG+RFV+HIKEGVY+EIVR+PLEKKNVVFLGDG
Sbjct: 243 DVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIPLEKKNVVFLGDG 302

Query: 333 MGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLS 392
           MGKTVITGSL  GQPG+STYNTATVGV+GDGFMASGLT QNTAG   HQAVAFRSDSDLS
Sbjct: 303 MGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTHQAVAFRSDSDLS 362

Query: 393 VIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKG 452
            I++CEF+G+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA++FQDCEI+V PRQ KPEKG
Sbjct: 363 FIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEIVVNPRQEKPEKG 422

Query: 453 ENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFI 512
           ENNAVTAHGRTDPAQ TGFVFQNCLINGT+EYMAL++SKP  HKN+LGRPWKEYSR VFI
Sbjct: 423 ENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLGRPWKEYSRVVFI 482

Query: 513 HCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSYSIQ 572
           HCN EA+++P GWMPWTGDFALKTLYYGEFENSG GS  S RV+WSS+IPA+HVY+YS+Q
Sbjct: 483 HCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQIPAEHVYTYSVQ 542

BLAST of Cp4.1LG16g00820 vs. TrEMBL
Match: A0A061DIW1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_001430 PE=4 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 2.0e-230
Identity = 395/559 (70.66%), Postives = 463/559 (82.83%), Query Frame = 1

Query: 21  KSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFT------NSPSSEETLRAC 80
           KST L LSM+ L  LS LSL  F +LSS+  +HHHH   H +      ++P S +   AC
Sbjct: 23  KSTCLLLSMSTLLLLSLLSLTLFFTLSSSSRNHHHHQDPHLSPKNSPVSTPPSSQIHLAC 82

Query: 81  KATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNR 140
           ++TRFP  C +SL+ S SLPPNPT+LQIIQSAIAVSS+ L + KSMVK ILDSS  + NR
Sbjct: 83  QSTRFPQPCETSLTQSSSLPPNPTSLQIIQSAIAVSSENLKTGKSMVKSILDSSKGNLNR 142

Query: 141 TTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSV 200
           T AA  CLE+L +S+YRI+  ND + RG+ KDARAWMSA+L Y YDCWSALKY NDT+ V
Sbjct: 143 TNAATTCLEILSYSDYRINSANDGLTRGKVKDARAWMSAALCYQYDCWSALKYVNDTKLV 202

Query: 201 NKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLGFSGGF 260
           ++TMAFL SL   +SNAL+M+ +YD  GND A+W  P+TERDGF+E  SGG+ LGF GGF
Sbjct: 203 DQTMAFLSSLTEHSSNALAMMVSYDNYGNDIASWAPPKTERDGFYESGSGGTDLGFKGGF 262

Query: 261 PSKVDASATVCKDGAGGCYKTVQEAVNAAPDNA-MGKRFVIHIKEGVYDEIVRVPLEKKN 320
           P ++    TVCKDG+GGCYKTVQ+AVNAAP NA   +RFVIHIKEGVY+E VRVP EKKN
Sbjct: 263 PPELQPDVTVCKDGSGGCYKTVQDAVNAAPHNAETTRRFVIHIKEGVYEETVRVPFEKKN 322

Query: 321 VVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAF 380
           VVFLGDGMGKT+ITGS NVGQPG++TYN+ATVGV+GDGFMASGLTI+NTAGPDAHQAVAF
Sbjct: 323 VVFLGDGMGKTIITGSANVGQPGMTTYNSATVGVLGDGFMASGLTIKNTAGPDAHQAVAF 382

Query: 381 RSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPR 440
           RSDSDLSVI++CEF+GNQDTLY HSLRQFYK C+IQGNVDFIFGNSASVFQDC +LV PR
Sbjct: 383 RSDSDLSVIENCEFIGNQDTLYVHSLRQFYKKCRIQGNVDFIFGNSASVFQDCIVLVAPR 442

Query: 441 QLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKE 500
           QLKPEKGENNA+TAHGRTDPAQ+TG VFQNCL+NGT EYM  YYSKPKVH+NFLGRPWKE
Sbjct: 443 QLKPEKGENNAITAHGRTDPAQSTGLVFQNCLLNGTDEYMGYYYSKPKVHRNFLGRPWKE 502

Query: 501 YSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQH 560
           YSRTVFI+C +EAL++P GW+PW+GD  LKTL+YGEF NSG GS  + RV WSS+IPAQ 
Sbjct: 503 YSRTVFINCFMEALITPDGWLPWSGDVGLKTLFYGEFGNSGPGSNVASRVPWSSQIPAQR 562

Query: 561 VYSYSIQNFIQGDEWIPSS 573
           VY+YS+QNFIQGD+WIP+S
Sbjct: 563 VYTYSVQNFIQGDQWIPTS 581

BLAST of Cp4.1LG16g00820 vs. TrEMBL
Match: F6I0G4_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_04s0044g01000 PE=4 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 7.8e-227
Identity = 403/555 (72.61%), Postives = 459/555 (82.70%), Query Frame = 1

Query: 29  MAALSFLSFLSLFFFLSLSSAVHHHHHHHSF-HF----TNSPSSE----ETLRACKATRF 88
           MA+L  L+ LSLF F SLSSA HHH   HS  HF    T  PS+     +  +AC ATR+
Sbjct: 1   MASLFLLALLSLFLFFSLSSASHHHSPRHSLPHFPQPITPQPSTASVPPQIHQACAATRY 60

Query: 89  PDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAK 148
           P+ C +SL  S  +PP+P  + +IQSA+ VS + L +A+SMVK ILD+S  + NRTTAAK
Sbjct: 61  PETCEASLIASDRVPPDPKPIDVIQSALWVSLENLKTAQSMVKDILDASARNLNRTTAAK 120

Query: 149 NCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMA 208
           NCLEVL  SEYRIS T +A+  G+ KDARAW+SA+LLY YDCWSALKYANDTQ VNKTM+
Sbjct: 121 NCLEVLHNSEYRISSTMEALPHGKIKDARAWVSAALLYQYDCWSALKYANDTQQVNKTMS 180

Query: 209 FLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGG--SGLGFSGGFPSK 268
           FLDSL+GL+SN LSM+A+YDI GND  +W  P+TERDGFWE S  G  SGLG  GG P+ 
Sbjct: 181 FLDSLLGLSSNGLSMMASYDIFGNDIGSWRPPKTERDGFWEPSGLGEESGLGVKGGVPTG 240

Query: 269 VDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFL 328
           +   ATVCKDG  GCYKTVQEAV+AAP NA  ++FVI I+EGVY+E VRVPLEKKNVVFL
Sbjct: 241 LPPDATVCKDG-NGCYKTVQEAVDAAPANAGDRKFVIRIREGVYEETVRVPLEKKNVVFL 300

Query: 329 GDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDS 388
           GDGMGKTVITGSLNVGQPGISTYNTATVGV GDGFMASGLT QNTAGPDAHQAVAFRS S
Sbjct: 301 GDGMGKTVITGSLNVGQPGISTYNTATVGVSGDGFMASGLTFQNTAGPDAHQAVAFRSGS 360

Query: 389 DLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKP 448
           DLSVI++CEFLGNQDTLYAHSLRQFYKSC IQGNVDFIFGNSAS+FQDC IL+RPRQLKP
Sbjct: 361 DLSVIENCEFLGNQDTLYAHSLRQFYKSCNIQGNVDFIFGNSASIFQDCLILIRPRQLKP 420

Query: 449 EKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRT 508
           EKGENNAVTAHGRTDPAQTTGFVFQNC++NGT +YM LYYS PKVHKNFLGRPWKE+SRT
Sbjct: 421 EKGENNAVTAHGRTDPAQTTGFVFQNCVVNGTDDYMKLYYSNPKVHKNFLGRPWKEFSRT 480

Query: 509 VFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSY 568
           VFI C LE LV+P GW+PW+GDFALKTLYYGEF NSG G+  S RV WSS+IPAQH+ +Y
Sbjct: 481 VFIRCFLEPLVTPQGWLPWSGDFALKTLYYGEFNNSGLGASLSARVTWSSQIPAQHLNTY 540

Query: 569 SIQNFIQGDEWIPSS 573
           S+QNFIQG+ WIP++
Sbjct: 541 SVQNFIQGNGWIPTT 554

BLAST of Cp4.1LG16g00820 vs. TrEMBL
Match: W9R8G4_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_020726 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 3.0e-226
Identity = 397/555 (71.53%), Postives = 463/555 (83.42%), Query Frame = 1

Query: 29  MAALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFTNSP-------SSEETLRACKATRFPD 88
           MA+L FLS +SL  F SLS + HH  H       + P       +S   +  CKATRFPD
Sbjct: 1   MASLLFLSIISLSLFFSLSHSSHHRGHSPPARVPHVPPSANPSTASSAVMHVCKATRFPD 60

Query: 89  QCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNC 148
            C  SL+    + P+ +  Q+I +AI VSS+ L +A+SMV+ ILDSS+ +PNR+ AA NC
Sbjct: 61  VCEGSLAR---VSPDASPAQVIAAAIGVSSENLRTAQSMVRAILDSSSGNPNRSIAAANC 120

Query: 149 LEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFL 208
           +EVLR SEYRIS  ++A+ RGR KDARAWMSA+LLY YDCWSALKYANDTQ VN+TM+ L
Sbjct: 121 VEVLRNSEYRISAASEALPRGRNKDARAWMSAALLYQYDCWSALKYANDTQLVNQTMSLL 180

Query: 209 DS-LIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWE-GSSGGSGLGFSGGFPSKVD 268
           D+ LI L+SNALSM+A+YD  GN+TA+W  PRTERDGFWE GS  GSGLG  GGFPSK+ 
Sbjct: 181 DTVLIRLSSNALSMMASYDNFGNETASWGPPRTERDGFWEKGSGSGSGLGSQGGFPSKLT 240

Query: 269 ASATVCKDGAGGCYKTVQEAVNAAPDNAMG-KRFVIHIKEGVYDEIVRVPLEKKNVVFLG 328
           A  TVCK G G  Y+ VQ+AVNAAP+  +G KRFVI +KEGVY+E VR+ +EKKNVV LG
Sbjct: 241 ADVTVCKGGGGCDYEKVQDAVNAAPNGVVGGKRFVIRVKEGVYEETVRIGIEKKNVVLLG 300

Query: 329 DGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSD 388
           DGMGKTVITGSLNVGQPGISTYNTATVGV+GDGFMA+GLTIQNTAGPDAHQAVAFRSDSD
Sbjct: 301 DGMGKTVITGSLNVGQPGISTYNTATVGVLGDGFMANGLTIQNTAGPDAHQAVAFRSDSD 360

Query: 389 LSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPE 448
           LS+I++CEF+GNQDTLYAHSLRQFYKSC+IQGNVDFIFGNSAS+FQDCEIL+RPRQLKPE
Sbjct: 361 LSIIENCEFIGNQDTLYAHSLRQFYKSCRIQGNVDFIFGNSASIFQDCEILIRPRQLKPE 420

Query: 449 KGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTV 508
           KGENNAVTAHGRTDPAQ+TGFVFQNCLINGT EYM LY SKP+VHKNFLGRPWKEYSRTV
Sbjct: 421 KGENNAVTAHGRTDPAQSTGFVFQNCLINGTDEYMKLYLSKPQVHKNFLGRPWKEYSRTV 480

Query: 509 FIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSYS 568
           FIHCNLEAL++P GWMPW+ DFALKTL+YGEFENSG GS  S R+ WSS+IPA+HVY+YS
Sbjct: 481 FIHCNLEALITPQGWMPWSQDFALKTLFYGEFENSGAGSDVSRRMTWSSQIPAEHVYAYS 540

Query: 569 IQNFIQGDEWIPSSQ 574
           +++FIQGDEWIP+++
Sbjct: 541 VESFIQGDEWIPTNE 552

BLAST of Cp4.1LG16g00820 vs. TAIR10
Match: AT5G09760.1 (AT5G09760.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 713.0 bits (1839), Expect = 1.5e-205
Identity = 361/552 (65.40%), Postives = 432/552 (78.26%), Query Frame = 1

Query: 29  MAALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFTN-SPSSEETLR---ACKATRFPDQCG 88
           M+++  L F SLF F S SS+  HHHHH+S   +  +PSS    +   AC ATR+PDQC 
Sbjct: 1   MSSILILLF-SLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCV 60

Query: 89  SSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNCLEV 148
           SSLS    +PP+P  +QII SAI+ S   L +A+S +K I+DSS  + NRT AA  CL++
Sbjct: 61  SSLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQL 120

Query: 149 LRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFLDSL 208
           L +SE+R   T+ A+ RG+ KDARAWMSA+L+Y YD WSALKY NDT  V +TM+FLD L
Sbjct: 121 LTYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGL 180

Query: 209 IGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGL----GFSGGFPSKVDA 268
           I +TSNALSM+ +YD  G++ A+WT P TERDGFWE +  G GL    G + GFPS +  
Sbjct: 181 IHVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKE 240

Query: 269 SATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDG 328
             TVCKDG  G YKTVQ+AVNAAP++   ++FVI I EGVY+E V VP EKKNVVF+GDG
Sbjct: 241 DVTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDG 300

Query: 329 MGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLS 388
           MGKTVITGSLN G PGI+TYNTATVGVVGDGFMA  LT QNTAGPDAHQAVAFRSDSD S
Sbjct: 301 MGKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFS 360

Query: 389 VIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKG 448
           +I++CEFLGNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA+VFQDCEIL+ PRQ+ PEKG
Sbjct: 361 LIENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKG 420

Query: 449 ENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFI 508
           E NAVTA GR DP+Q+TGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI
Sbjct: 421 EKNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFI 480

Query: 509 HCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSYSIQ 568
            CNLEAL++P GW+PW+GDFALKTLYYGE +N+G GS  S+RV WSS+IP +HV+ YS+ 
Sbjct: 481 GCNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVA 540

Query: 569 NFIQGDEWIPSS 573
           NFIQ DEW   S
Sbjct: 541 NFIQADEWASMS 550

BLAST of Cp4.1LG16g00820 vs. TAIR10
Match: AT5G64640.1 (AT5G64640.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 647.5 bits (1669), Expect = 7.8e-186
Identity = 332/566 (58.66%), Postives = 414/566 (73.14%), Query Frame = 1

Query: 20  AKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHHHH--HHSFHFTNSPSSE--------- 79
           +++  L +  A+   L  +SL  + ++S +  +HH+  H +    + P  E         
Sbjct: 32  SRNGILIIIAASCILLLLISLLIYATVSKSSRNHHNPSHQTPTSDDHPPPETPPSPPPIA 91

Query: 80  ETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSS 139
           +   AC ATRFPD C +SLS    +PP+P  +QII SAI+VS + L S +S ++ ILDSS
Sbjct: 92  QIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDSS 151

Query: 140 TDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYA 199
             + NRT  A  CLE+L +S++R   T+ AV  G  KDARAWMSA+L Y +DCWS LK  
Sbjct: 152 AGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKTV 211

Query: 200 NDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWE--GSSGGS 259
           NDT+ V  T+ F + L+ LT NALSM+ ++D  G+D  +W  P TERDGFWE  G S GS
Sbjct: 212 NDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLGS 271

Query: 260 GLGFSG--GFPSKVDASATVCKDGAGGC-YKTVQEAVNAAPDNAMGKRFVIHIKEGVYDE 319
           G G     GFPS +    TVCK+G   C YKTVQEAV++APD     +FVI I+EGVY+E
Sbjct: 272 GTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYEE 331

Query: 320 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTA 379
            VRVP EKKNVVF+GDGMGKTVITGSLNVGQPG++T+ +ATVGV+GDGFMA  LTI+NTA
Sbjct: 332 TVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENTA 391

Query: 380 GPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVF 439
           G DAHQAVAFRSDSD SV+++CEFLGNQDTLYAHSLRQFYK C+IQGNVDFIFGNSA+VF
Sbjct: 392 GADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAVF 451

Query: 440 QDCEILVRPRQLKPEK-GENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKV 499
           QDC+IL+  +  K E+ G NNA+TAHGR D +Q+TGFVF NC INGT+EYM  + + P+ 
Sbjct: 452 QDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPEG 511

Query: 500 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 559
           HKNFLGRPWKE+SRTVF++CNLE+L+SP GWMPW GDFALKTLYYGE++N+G GS  S R
Sbjct: 512 HKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSSR 571

Query: 560 VEWSSKIPAQHVYSYSIQNFIQGDEW 569
           V WSS+IP +HV  YS+ NFIQ DEW
Sbjct: 572 VPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of Cp4.1LG16g00820 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 311.2 bits (796), Expect = 1.3e-84
Identity = 193/532 (36.28%), Postives = 287/532 (53.95%), Query Frame = 1

Query: 74  CKATRFPDQCGSSLSHSPSLPPNP---------TTLQIIQSAIAVSSDGLAS-------- 133
           C  T + + C +SL  +      P          T++ I+ +I  +S  L +        
Sbjct: 59  CAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDT 118

Query: 134 --AKSMVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSAS 193
             A  + +++++ +TD        K CL+   F  + I    D V     +D R W+S S
Sbjct: 119 KGALELCEKLMNDATDD------LKKCLD--NFDGFSIPQIEDFV-----EDLRVWLSGS 178

Query: 194 LLYHYDCWSALKYAN-----DTQSVNKTMAFLDSL-IGLTSNALSMLAAYDISGNDTAAW 253
           + Y   C    +  N     D Q + KT   L S  + + +N  ++L  ++++G      
Sbjct: 179 IAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLG 238

Query: 254 TLPR---TERDGF--WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAA 313
              R   +  DG   W G +    +   GG    V A+  V  DG+G  YKT+ EA+NA 
Sbjct: 239 KYARKLLSAEDGIPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-YKTINEALNAV 298

Query: 314 PDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTA 373
           P  A  K FVI+IK+GVY+E V V  +  +V F+GDG  KT ITGSLN     + TY TA
Sbjct: 299 P-KANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTA 358

Query: 374 TVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFY 433
           TV + GD F A  +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDTLY HS RQF+
Sbjct: 359 TVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFF 418

Query: 434 KSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQN 493
           + C + G VDFIFG+   V Q+C I+VR    KP K ++  +TA GR+D  ++TG V QN
Sbjct: 419 RDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRESTGLVLQN 478

Query: 494 CLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALK 553
           C I G   Y+ +      ++K +LGRPWKE+SRT+ +   ++ ++ PAGW+PW GDFAL 
Sbjct: 479 CHITGEPAYIPV----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALN 538

Query: 554 TLYYGEFENSGTGSKTSERVEWS--SKIPAQHVYSYSIQNFIQGDEWIPSSQ 574
           TLYY E+EN+G GS  ++RV+W    K+  +    ++   F++G+ WIP ++
Sbjct: 539 TLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNR 563

BLAST of Cp4.1LG16g00820 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 305.8 bits (782), Expect = 5.6e-83
Identity = 188/502 (37.45%), Postives = 276/502 (54.98%), Query Frame = 1

Query: 74  CKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPN 133
           C  T  P  C   L+H+ +  P  +  + ++ ++ +  D    AK+    +     D+  
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRDTRE 93

Query: 134 RTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQS 193
           +  A ++C+++   +  +I+ T D   +    DA+ W+S +L     C +       T  
Sbjct: 94  KA-AWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVT-- 153

Query: 194 VNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLGFSGG 253
                   D ++ L SN +S L    ++ N       P  E+DGF      G        
Sbjct: 154 --------DIVLPLMSNNVSNLLCNTLAINKVPFNYTP-PEKDGFPSWVKPGDRKLLQSS 213

Query: 254 FPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKN 313
            P     +A V KDG+G  +KT++EA++AA  +    RFVI++K+GVY E   + + KKN
Sbjct: 214 TPKD---NAVVAKDGSGN-FKTIKEAIDAASGSG---RFVIYVKQGVYSE--NLEIRKKN 273

Query: 314 VVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAF 373
           V+  GDG+GKT+ITGS +VG  G +T+N+ATV  VGDGF+A G+T +NTAG    QAVA 
Sbjct: 274 VMLRGDGIGKTIITGSKSVGG-GTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVAL 333

Query: 374 RSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPR 433
           RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A+V Q+C I  R  
Sbjct: 334 RSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFAR-- 393

Query: 434 QLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKE 493
             +P + + N +TA GR+DP Q TG +  N  +    +   +  S     K +LGRPW++
Sbjct: 394 --RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPWRQ 453

Query: 494 YSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSS---KIP 553
           YSRTVF+  +L++L+ P GW+ W G+FALKTL+Y EF+N+G G+ TS RV W        
Sbjct: 454 YSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGS 504

Query: 554 AQHVYSYSIQNFIQGDEWIPSS 573
           A     +++  F+ G  WIPSS
Sbjct: 514 ASEASKFTVGTFLAGGSWIPSS 504

BLAST of Cp4.1LG16g00820 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 302.4 bits (773), Expect = 6.1e-82
Identity = 152/337 (45.10%), Postives = 211/337 (62.61%), Query Frame = 1

Query: 239 WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKE 298
           W G      +   GG P  V A+A V +DG G  +KT+ +A+NA P       F+IHIKE
Sbjct: 235 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKVP-FIIHIKE 294

Query: 299 GVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLT 358
           G+Y E V V  +  +V F+GDG  KT+ITGSLN G   + T+ TAT+ + GD F A  + 
Sbjct: 295 GIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIG 354

Query: 359 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 418
           I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G VDFIFG+
Sbjct: 355 IENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGD 414

Query: 419 SASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYS 478
           +  + Q+C+I+VR    KP KG+   VTA GR++  ++TG V   C I G   Y+ +   
Sbjct: 415 AKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPM--- 474

Query: 479 KPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSK 538
              V+K +LGRPWKE+SRT+ +   ++ ++ PAGW+PW+GDFALKTLYY E  N+G GS 
Sbjct: 475 -KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSN 534

Query: 539 TSERVEWS--SKIPAQHVYSYSIQNFIQGDEWIPSSQ 574
            ++RV+W    K+  Q    Y+   F++GD WIP +Q
Sbjct: 535 QAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQ 561

BLAST of Cp4.1LG16g00820 vs. NCBI nr
Match: gi|778670202|ref|XP_004147375.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus])

HSP 1 Score: 970.7 bits (2508), Expect = 1.1e-279
Identity = 491/584 (84.08%), Postives = 533/584 (91.27%), Query Frame = 1

Query: 6   PRTTPFPFPFQFATAKSTFLFLSMAALSFLSFLSLFFF-----LSLSSAVHHHHHHHSF- 65
           PR   FPF      +KST LFLSMA+LSFLSFLSLFFF     LS SSA HHHH H    
Sbjct: 4   PRKPTFPF------SKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLP 63

Query: 66  -HFTNSPSSEETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAK 125
            HFTN PS ++ L ACK+TRFPDQCGSSLSHS SLPPNP+ LQI+QSAIA+SSDGL +A+
Sbjct: 64  SHFTNYPS-DQILHACKSTRFPDQCGSSLSHS-SLPPNPSPLQILQSAIALSSDGLTTAQ 123

Query: 126 SMVKQILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYH 185
           S+VKQILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+  GRTKDARAW+SASLLYH
Sbjct: 124 SLVKQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYH 183

Query: 186 YDCWSALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGF 245
           YDCWSALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWT PRTERDGF
Sbjct: 184 YDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGF 243

Query: 246 WEGSSGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKE 305
           WEG  GGSGLGFSGGFP+KV A ATVCKDG+GGCY ++Q+AV+AAP+NA  +RFVIHIKE
Sbjct: 244 WEGG-GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKE 303

Query: 306 GVYDEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLT 365
           GVY+EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPG+STYNTAT+GVVGDGFMASGLT
Sbjct: 304 GVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLT 363

Query: 366 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 425
           IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN
Sbjct: 364 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 423

Query: 426 SASVFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYS 485
           SAS+FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQ+TGFVFQNCLINGTQEYM+LYYS
Sbjct: 424 SASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYS 483

Query: 486 KPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSK 545
           KP+VHKNFLGRPWKEYSRTVFIHCNLEALVSP+GWMPW+GDFALKTL+YGEFENSG GSK
Sbjct: 484 KPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSK 543

Query: 546 TSERVEWSSKIPAQHVYSYSIQNFIQGDEWIPSSQSSEESKTIN 583
           TSERV WSS+IPAQHV+SYS++NFIQGDEWIPSS+SS+E K +N
Sbjct: 544 TSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLN 578

BLAST of Cp4.1LG16g00820 vs. NCBI nr
Match: gi|659121958|ref|XP_008460902.1| (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 970.7 bits (2508), Expect = 1.1e-279
Identity = 485/580 (83.62%), Postives = 529/580 (91.21%), Query Frame = 1

Query: 11  FPFPFQFATAKSTFLFLSMAALSFLSFLSL-FFFLSLS-----SAVHHHHHHHSF--HFT 70
           FP    F  +KST LFLSMA+LSFLSFLSL FFF SLS     SAV HHH H     HFT
Sbjct: 3   FPRKPTFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFT 62

Query: 71  NSPSSEETLRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVK 130
           N+PS ++ L ACK+TRFPDQCGSSLSHS   PPNP+ LQI+QSAIA+SSDGL +A+S+VK
Sbjct: 63  NTPS-DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLVK 122

Query: 131 QILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCW 190
           QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+ RGRTKDARAW+SASLLYHYDCW
Sbjct: 123 QILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCW 182

Query: 191 SALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGS 250
           SALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDI GNDTAAWT PRTERDGFWEG 
Sbjct: 183 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGG 242

Query: 251 SGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYD 310
            GGSGLGFSGGFP+KV A ATVCKDG+GGCY ++Q+AV+AAP+NA  +RFVIHIKEGVY+
Sbjct: 243 GGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYE 302

Query: 311 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNT 370
           EIVRVPLEKKNVVFLGDGMGKTVITGSLN GQPGISTYNTAT+GV+GDGFMASGLTIQNT
Sbjct: 303 EIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNT 362

Query: 371 AGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASV 430
           AGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+
Sbjct: 363 AGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASI 422

Query: 431 FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKV 490
           FQDCEIL+RPRQLKPEKGENNA+TAHGRTDPAQ+TGFVFQNCLINGTQEYM+LYYSKPKV
Sbjct: 423 FQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKV 482

Query: 491 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 550
           HKNFLGRPWKEYSRTVFIHCNLEALVSP+GWMPW+GDFALKTL+YGEFENSG GSKTSER
Sbjct: 483 HKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSER 542

Query: 551 VEWSSKIPAQHVYSYSIQNFIQGDEWIPSSQSSEESKTIN 583
           V WSS+IPAQHV+SYS+QNFIQGDEWIPSS+SS + K +N
Sbjct: 543 VGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLN 581

BLAST of Cp4.1LG16g00820 vs. NCBI nr
Match: gi|1009146861|ref|XP_015891098.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Ziziphus jujuba])

HSP 1 Score: 860.5 bits (2222), Expect = 1.7e-246
Identity = 430/566 (75.97%), Postives = 488/566 (86.22%), Query Frame = 1

Query: 14  PFQFATAKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHHHHHHSFHFTNSPSSEET--- 73
           PF  + +K TFL +SMA+L  LS +SL  F SLSSA +HH HH+       P+S      
Sbjct: 11  PFPLSLSKPTFLLISMASLLILSLISLLLFFSLSSASNHHSHHNPKIIPTVPTSSSLPAA 70

Query: 74  -LRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSST 133
             +ACKATRFP+QC +SLS SP LPPNP++++I+QSAI+VSS+ L +A SMVK ILDSS+
Sbjct: 71  IQQACKATRFPEQCQTSLSQSPHLPPNPSSVEIVQSAISVSSENLKTALSMVKSILDSSS 130

Query: 134 DSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYAN 193
            S NRTTAA NCLEVL  SEYR SL   A   G+ KDARAWMSA+LL+ YDCWSALKYAN
Sbjct: 131 GSQNRTTAANNCLEVLHNSEYRTSLAGKAFPAGKMKDARAWMSAALLFQYDCWSALKYAN 190

Query: 194 DTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFW-EGSSGGSGL 253
           DT+ VN+TM+FLDSLIGL+SNALSM+A+YD  G  T +W  P+TERDGFW  GS GGS L
Sbjct: 191 DTKMVNETMSFLDSLIGLSSNALSMMASYDNFGAKTESWVPPKTERDGFWGNGSGGGSEL 250

Query: 254 GFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAM-GKRFVIHIKEGVYDEIVRV 313
           G  GGFPSK+ A ATVCKDG GGCYKTVQ+AVNAAPDNA+ GK+FVIHIKEGVY+E VRV
Sbjct: 251 GSGGGFPSKLTADATVCKDGRGGCYKTVQDAVNAAPDNAVVGKKFVIHIKEGVYEETVRV 310

Query: 314 PLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDA 373
           PLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGV+GDGFMASGLTI+NTAGPDA
Sbjct: 311 PLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVLGDGFMASGLTIKNTAGPDA 370

Query: 374 HQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCE 433
           HQAVAFRSDSDLSVIQ+CEFLGNQDTLYAH+LRQFYKSC+IQGNVDFIFGNSASVFQDC+
Sbjct: 371 HQAVAFRSDSDLSVIQNCEFLGNQDTLYAHALRQFYKSCRIQGNVDFIFGNSASVFQDCQ 430

Query: 434 ILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFL 493
           I V PRQLKPEKGENNAVTA+GRTDPAQ+TGFVFQNCLINGT+EYM LYYSKPKVHKNFL
Sbjct: 431 IFVAPRQLKPEKGENNAVTANGRTDPAQSTGFVFQNCLINGTEEYMKLYYSKPKVHKNFL 490

Query: 494 GRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSS 553
           GRPWKEYSRTVFI+CN+E+L++P GWMPW+GDFALKTL+YGEF NSG GS  S+RV WSS
Sbjct: 491 GRPWKEYSRTVFINCNIESLLAPQGWMPWSGDFALKTLFYGEFGNSGPGSNLSQRVAWSS 550

Query: 554 KIPAQHVYSYSIQNFIQGDEWIPSSQ 574
           +IPA+HV +YS+QNFIQGDEWIP+SQ
Sbjct: 551 RIPAEHVNAYSVQNFIQGDEWIPTSQ 576

BLAST of Cp4.1LG16g00820 vs. NCBI nr
Match: gi|1000970516|ref|XP_002517239.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 isoform X1 [Ricinus communis])

HSP 1 Score: 826.6 bits (2134), Expect = 2.7e-236
Identity = 412/564 (73.05%), Postives = 470/564 (83.33%), Query Frame = 1

Query: 19  TAKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHHHHHH-------SFHFTNSPSSEETL 78
           T KS F+ +SMA+    S LSL+ FLS +S    HH +        S   +  P   E L
Sbjct: 14  TQKSFFISISMASFLSFSLLSLYLFLSSTSVTSRHHTYRPKLPPPSSLSVSTPP---EIL 73

Query: 79  RACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDS 138
           +ACKATRFPD C SSL+ S S+P NPT LQIIQS++++S+  L +A+SMVK ILDSS  +
Sbjct: 74  QACKATRFPDTCASSLTSSDSVPKNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGN 133

Query: 139 PNRTTAAKNCLEVLRFSEYRISLTND-AVRRGRTKDARAWMSASLLYHYDCWSALKYAND 198
            NRT AA+NCLEVL  SEYRIS T D ++ RGRTKDARAWMSA+LLY YDCWSALKYAND
Sbjct: 134 INRTNAARNCLEVLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYAND 193

Query: 199 TQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLG- 258
           T   NKTM+FLDSL+ LTSN+LSM+ +YDI GNDT  WT P+TERDGFWEGS  GSG G 
Sbjct: 194 TSDTNKTMSFLDSLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGL 253

Query: 259 -FSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVP 318
            F G FPSK+ A  TVCKDG+ GCYKTVQEAVN APDN MG+RFV+HIKEGVY+EIVR+P
Sbjct: 254 VFRGVFPSKLTADVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIP 313

Query: 319 LEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAH 378
           LEKKNVVFLGDGMGKTVITGSL  GQPG+STYNTATVGV+GDGFMASGLT QNTAG   H
Sbjct: 314 LEKKNVVFLGDGMGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTH 373

Query: 379 QAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEI 438
           QAVAFRSDSDLS I++CEF+G+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA++FQDCEI
Sbjct: 374 QAVAFRSDSDLSFIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEI 433

Query: 439 LVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLG 498
           +V PRQ KPEKGENNAVTAHGRTDPAQ TGFVFQNCLINGT+EYMAL++SKP  HKN+LG
Sbjct: 434 VVNPRQEKPEKGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLG 493

Query: 499 RPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSK 558
           RPWKEYSR VFIHCN EA+++P GWMPWTGDFALKTLYYGEFENSG GS  S RV+WSS+
Sbjct: 494 RPWKEYSRVVFIHCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQ 553

Query: 559 IPAQHVYSYSIQNFIQGDEWIPSS 573
           IPA+HVY+YS+QNFIQGDEWIP+S
Sbjct: 554 IPAEHVYTYSVQNFIQGDEWIPTS 574

BLAST of Cp4.1LG16g00820 vs. NCBI nr
Match: gi|223543610|gb|EEF45139.1| (Pectinesterase-2 precursor, putative [Ricinus communis])

HSP 1 Score: 820.5 bits (2118), Expect = 1.9e-234
Identity = 409/552 (74.09%), Postives = 464/552 (84.06%), Query Frame = 1

Query: 33  SFLSF--LSLFFFLSLSSAVHHHHHHH-------SFHFTNSPSSEETLRACKATRFPDQC 92
           SFLSF  LSL+ FLS +S    HH +        S   +  P   E L+ACKATRFPD C
Sbjct: 3   SFLSFSLLSLYLFLSSTSVTSRHHTYRPKLPPPSSLSVSTPP---EILQACKATRFPDTC 62

Query: 93  GSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNRTTAAKNCLE 152
            SSL+ S S+P NPT LQIIQS++++S+  L +A+SMVK ILDSS  + NRT AA+NCLE
Sbjct: 63  ASSLTSSDSVPKNPTPLQIIQSSVSISATNLKTAQSMVKSILDSSAGNINRTNAARNCLE 122

Query: 153 VLRFSEYRISLTND-AVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSVNKTMAFLD 212
           VL  SEYRIS T D ++ RGRTKDARAWMSA+LLY YDCWSALKYANDT   NKTM+FLD
Sbjct: 123 VLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSALKYANDTSDTNKTMSFLD 182

Query: 213 SLIGLTSNALSMLAAYDISGNDTAAWTLPRTERDGFWEGSSGGSGLG--FSGGFPSKVDA 272
           SL+ LTSN+LSM+ +YDI GNDT  WT P+TERDGFWEGS  GSG G  F G FPSK+ A
Sbjct: 183 SLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEWGSGGGLVFRGVFPSKLTA 242

Query: 273 SATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNVVFLGDG 332
             TVCKDG+ GCYKTVQEAVN APDN MG+RFV+HIKEGVY+EIVR+PLEKKNVVFLGDG
Sbjct: 243 DVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVYNEIVRIPLEKKNVVFLGDG 302

Query: 333 MGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLS 392
           MGKTVITGSL  GQPG+STYNTATVGV+GDGFMASGLT QNTAG   HQAVAFRSDSDLS
Sbjct: 303 MGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQNTAGAPTHQAVAFRSDSDLS 362

Query: 393 VIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQLKPEKG 452
            I++CEF+G+QDTLYAHSLRQFYKSC+I+GNVDFIFGNSA++FQDCEI+V PRQ KPEKG
Sbjct: 363 FIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAAIFQDCEIVVNPRQEKPEKG 422

Query: 453 ENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKVHKNFLGRPWKEYSRTVFI 512
           ENNAVTAHGRTDPAQ TGFVFQNCLINGT+EYMAL++SKP  HKN+LGRPWKEYSR VFI
Sbjct: 423 ENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPGAHKNYLGRPWKEYSRVVFI 482

Query: 513 HCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSKIPAQHVYSYSIQ 572
           HCN EA+++P GWMPWTGDFALKTLYYGEFENSG GS  S RV+WSS+IPA+HVY+YS+Q
Sbjct: 483 HCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSGRVKWSSQIPAEHVYTYSVQ 542