Cucsa.249950 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.249950 vs. Swiss-Prot
Match: PME51_ARATH (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana GN=PME51 PE=2 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 5.3e-183
Identity = 324/543 (59.67%), Postives = 406/543 (74.77%), Query Frame = 1

Query: 5   LFLFFLPLHFSSAAHQ-------------TLLPDIAAACNATRFPDQCASSISQSSRLPQ 64
           LFLF  P   S   H              +L   I  ACNATR+PDQC SS+S+  R+P 
Sbjct: 11  LFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSLSEQGRVPP 70

Query: 65  NPTSLQIIQAALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRIS 124
           +P  +QII +A+  S  NLKTA+S +K+I+DS+  + N NRT  A +C+  L YSE+R  
Sbjct: 71  DPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSS--VGNLNRTNAANTCLQLLTYSEHRTQ 130

Query: 125 LSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALS 184
            ++ A+  G+IKDARAW+SA+L+YQ    S LKYVNDT +V +TM FLD L  ++SNALS
Sbjct: 131 STDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHVTSNALS 190

Query: 185 MVVAYDNFGNETAAWRPPRTERDGFWERSS-GTG-EVVRGVRI--PTKTEANVTVCKDGS 244
           M+V+YDNFG+  A+W  P TERDGFWE++  G G +   G+ +  P+  + +VTVCKDG 
Sbjct: 191 MMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVTVCKDGK 250

Query: 245 AGCYRTVQAAVDAAPGESAVR-FVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGS 304
            G Y+TVQ AV+AAP ++ +R FVI I EGVYEE+V V  EKKN+VF+GDGMGKTVITGS
Sbjct: 251 CG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGKTVITGS 310

Query: 305 LNVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFIS 364
           LN G PGI+TYN+ATVGV+GDGF+A +LT QNTAGPDAHQAVAFRSDSD S+I+NCEF+ 
Sbjct: 311 LNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSLIENCEFLG 370

Query: 365 NQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHG 424
           NQDTLYAH LRQFY +CRI GNVDFIFGNSA++F +C ILI P Q+NP+ G++NA+TA G
Sbjct: 371 NQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGEKNAVTAQG 430

Query: 425 RTDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVS 484
           R DP+QSTGFVF NCLINGTEE+M L+ ANP+ H+ FLGRPWK+YSRTVFI C LEAL++
Sbjct: 431 RIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIGCNLEALIT 490

Query: 485 SSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWI 530
             GW+ WSGD  L TLYYGE +N G GS+ S+RV+WS+QIP E+V  YSV NFIQ +EW 
Sbjct: 491 PDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVANFIQADEWA 550

BLAST of Cucsa.249950 vs. Swiss-Prot
Match: PME64_ARATH (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana GN=PME64 PE=2 SV=2)

HSP 1 Score: 604.4 bits (1557), Expect = 1.2e-171
Identity = 300/511 (58.71%), Postives = 385/511 (75.34%), Query Frame = 1

Query: 23  LPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILD 82
           +  I  ACNATRFPD C +S+S+  ++P +P  +QII +A+ +S +NLK+ +S +++ILD
Sbjct: 90  IAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILD 149

Query: 83  SAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSG 142
           S+    NRNRT  A  C++ L YS++R   ++ A+  G IKDARAW+SA+L YQ  C SG
Sbjct: 150 SSAG--NRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSG 209

Query: 143 LKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGFWERS-- 202
           LK VNDT++V  T+ F + L  L+ NALSM++++D+FG++  +W  P TERDGFWE++  
Sbjct: 210 LKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGP 269

Query: 203 ---SGTGEVVRGVRIPTKTEANVTVCKDGSAGC-YRTVQAAVDAAPGES-AVRFVIHIKE 262
              SGTG     +  P+    +VTVCK+G   C Y+TVQ AVD+AP  +  V+FVI I+E
Sbjct: 270 SLGSGTGTEA-SLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIRE 329

Query: 263 GVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELT 322
           GVYEE+VRV  EKKN+VF+GDGMGKTVITGSLNVGQPG++T+ SATVGVLGDGF+A +LT
Sbjct: 330 GVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLT 389

Query: 323 IQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGN 382
           I+NTAG DAHQAVAFRSDSD S+++NCEF+ NQDTLYAHSLRQFY  CRI GNVDFIFGN
Sbjct: 390 IENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGN 449

Query: 383 SASMFLNCHILI-RPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYY 442
           SA++F +C ILI   H    Q G  NAITAHGR D +QSTGFVF NC INGTEE+M  + 
Sbjct: 450 SAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQ 509

Query: 443 ANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGS 502
           ANP+ H+ FLGRPWKE+SRTVF++C LE+L+S  GWM W+GD  L TLYYGE++N G GS
Sbjct: 510 ANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGS 569

Query: 503 NSSKRVAWSNQIPAEYVFSYSVQNFIQGNEW 526
             S RV WS++IP ++V  YSV NFIQ +EW
Sbjct: 570 VRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of Cucsa.249950 vs. Swiss-Prot
Match: PME2_CITSI (Pectinesterase 2 OS=Citrus sinensis GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.2e-86
Identity = 197/512 (38.48%), Postives = 283/512 (55.27%), Query Frame = 1

Query: 25  DIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILDSA 84
           ++ + C  T  P  C   ++Q + +          + +L L+ +   TA+S    +    
Sbjct: 27  EVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGSKC 86

Query: 85  EKIPNRNRTEKAL--SCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSG 144
                RN  EKA    C +    +  +++ ++++ P     D + WLS +L     CR+ 
Sbjct: 87  -----RNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRAS 146

Query: 145 LKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGF--WERS 204
           L+ +     V    L  +N+T L SN LS+        N+     P  + +DGF  W + 
Sbjct: 147 LEDLGVPEYV--LPLLSNNVTKLISNTLSL--------NKVPYNEP--SYKDGFPTWVKP 206

Query: 205 SGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESAVRFVIHIKEGVYEE 264
            G  ++++     T   AN+ V +DGS G  +T+Q AV AA      R+VI+IK G Y E
Sbjct: 207 -GDRKLLQ-----TTPRANIVVAQDGS-GNVKTIQEAVAAASRAGGSRYVIYIKAGTYNE 266

Query: 265 SVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELTIQNTA 324
           ++ V +  KN++F+GDG+GKT+ITGS +VG  G +T+ SATV V+GD FIA ++TI+NTA
Sbjct: 267 NIEVKL--KNIMFVGDGIGKTIITGSKSVGG-GATTFKSATVAVVGDNFIARDITIRNTA 326

Query: 325 GPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMF 384
           GP+ HQAVA RS SDLS+   C F   QDTLY HS RQFY  C I G VDFIFGN+A + 
Sbjct: 327 GPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVL 386

Query: 385 LNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYYANPQRH 444
            NC+I  R           N +TA GRTDP QSTG +  NC +    +   +        
Sbjct: 387 QNCNIFARKPP-----NRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPV----QSSV 446

Query: 445 RTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRV 504
           +TFLGRPWK+YSRTV+I   L++L++ +GWM WSGD  LNTLYY E+ N G GS+++ RV
Sbjct: 447 KTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRV 502

Query: 505 AWSNQ---IPAEYVFSYSVQNFIQGNEWIPSS 530
            W           V  ++V NFI GN W+P++
Sbjct: 507 KWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 502

BLAST of Cucsa.249950 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.9e-80
Identity = 188/536 (35.07%), Postives = 280/536 (52.24%), Query Frame = 1

Query: 20  QTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLS----SDNLK---- 79
           +T    + A C  T + + C +S+ ++S  P +   L +I+    ++     D++K    
Sbjct: 49  KTATTAVEAVCAPTDYKETCVNSLMKAS--PDSTQPLDLIKLGFNVTIRSIEDSIKKASV 108

Query: 80  --TAKSM----VKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDA 139
             TAK+      K  L+  EK+ N + T+    C+D   +  + I    D +     +D 
Sbjct: 109 ELTAKAANDKDTKGALELCEKLMN-DATDDLKKCLD--NFDGFSIPQIEDFV-----EDL 168

Query: 140 RAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNF------ 199
           R WLS S+ YQ  C    +  N     D   +F  +   L+SN L+M+    N       
Sbjct: 169 RVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRE-LTSNGLAMITNISNLLGEFNV 228

Query: 200 ----GNETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTK--TEANVTVCKDGSAGCYRT 259
               G+     R   +  DG     S  G   R + + TK   +ANV V  DGS G Y+T
Sbjct: 229 TGVTGDLGKYARKLLSAEDGI---PSWVGPNTRRL-MATKGGVKANVVVAHDGS-GQYKT 288

Query: 260 VQAAVDAAPGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPG 319
           +  A++A P  +   FVI+IK+GVY E V V  +  ++ F+GDG  KT ITGSLN     
Sbjct: 289 INEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGK 348

Query: 320 ISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYA 379
           + TY +ATV + GD F A  +  +NTAGP+ HQAVA R  +DL++  NC+    QDTLY 
Sbjct: 349 VKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYV 408

Query: 380 HSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQS 439
           HS RQF+  C + G VDFIFG+   +  NC+I++R     P       ITA GR+D  +S
Sbjct: 409 HSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRES 468

Query: 440 TGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAW 499
           TG V QNC I G   ++ +   N    + +LGRPWKE+SRT+ +   ++ ++  +GW+ W
Sbjct: 469 TGLVLQNCHITGEPAYIPVKSIN----KAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPW 528

Query: 500 SGDIGLNTLYYGEFENFGTGSNSSKRVAWS--NQIPAEYVFSYSVQNFIQGNEWIP 528
           +GD  LNTLYY E+EN G GSN ++RV W    ++  +    ++   F++GN WIP
Sbjct: 529 NGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIP 560

BLAST of Cucsa.249950 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 3.2e-79
Identity = 181/523 (34.61%), Postives = 280/523 (53.54%), Query Frame = 1

Query: 21  TLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSL--QIIQAALYLSSDNLKTAKSMVK 80
           T +  I   C  T + + C  ++ + ++   +P  L      A +   SD  K +++M++
Sbjct: 52  TSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIE 111

Query: 81  AILDSAEKIPNRNRTEKALS-CMDALGYSEYRISLSNDAI---PLGRIKDA----RAWLS 140
              D         R + AL  C + + Y+   +S S + +      ++ +A    R WLS
Sbjct: 112 LQKDP--------RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLS 171

Query: 141 ASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPR 200
           A++ ++  C  G +        +     L     L+ N L+MV    N+  +      P 
Sbjct: 172 ATISHEQTCLDGFQGTQGNAG-ETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQI---PE 231

Query: 201 TERDGFWERSSGTGEVVRGVRI----PTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESA 260
                   +   +    R  R+     ++ + ++ V +DGS G Y+T+  A++  P +  
Sbjct: 232 MNSRRLLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGS-GQYKTINEALNFVPKKKN 291

Query: 261 VRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLG 320
             FV+HIKEG+Y+E V+V     +LVF+GDG  KTVI+GS +  + GI+TY +ATV ++G
Sbjct: 292 TTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSY-KDGITTYKTATVAIVG 351

Query: 321 DGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRIL 380
           D FIA  +  +NTAG   HQAVA R  +D SI  NC+F   QDTLYAHS RQFY  C I 
Sbjct: 352 DHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTIS 411

Query: 381 GNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGT 440
           G +DF+FG++A++F NC +L+R     P L     ITAHGR DP +STGFV Q C I G 
Sbjct: 412 GTIDFLFGDAAAVFQNCTLLVR----KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGE 471

Query: 441 EEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGE 500
            +++A+     ++ +T+LGRPWKEYSRT+ ++  +   V   GW  W G+ GLNTL+Y E
Sbjct: 472 PDYLAV----KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSE 531

Query: 501 FENFGTGSNSSKRVAWS--NQIPAEYVFSYSVQNFIQGNEWIP 528
            +N G G+  +KRV W    ++  E +  ++   +IQG+ WIP
Sbjct: 532 VQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIP 552

BLAST of Cucsa.249950 vs. TrEMBL
Match: A0A0A0LMY9_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G302140 PE=4 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 1.6e-308
Identity = 528/529 (99.81%), Postives = 528/529 (99.81%), Query Frame = 1

Query: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60
           MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ
Sbjct: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60

Query: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDA 240
           NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGS GCYRTVQAAVDA
Sbjct: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSEGCYRTVQAAVDA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420

Query: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480
           CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN
Sbjct: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 529

BLAST of Cucsa.249950 vs. TrEMBL
Match: A0A0A0LQ80_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_2G302130 PE=4 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.6e-210
Identity = 368/521 (70.63%), Postives = 427/521 (81.96%), Query Frame = 1

Query: 10  LPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDN 69
           LP HF++     +L     AC +TRFPDQC SS+S SS LP NP+ LQI+Q+A+ LSSD 
Sbjct: 56  LPSHFTNYPSDQILH----ACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDG 115

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L TA+S+VK ILDS+    N NRT  A +C++ L  S+YRISL+NDA+  GR KDARAWL
Sbjct: 116 LTTAQSLVKQILDSSAT--NLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWL 175

Query: 130 SASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPP 189
           SASLLY   C S LKY NDT+ VD+TM FLD+L  LSSNALSM+VAYD +GN+TAAW PP
Sbjct: 176 SASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPP 235

Query: 190 RTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESAVR- 249
           RTERDGFWE   G+G    G   P K  A+ TVCKDGS GCY ++Q AVDAAP  +A R 
Sbjct: 236 RTERDGFWEGGGGSGLGFSG-GFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARR 295

Query: 250 FVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVYEE VRV +EKKN+VFLGDGMGKTVITGSLNVGQPG+STYN+AT+GV+GDG
Sbjct: 296 FVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDG 355

Query: 310 FIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPDAHQAVAFRSDSDLS+I +CEF+ NQDTLYAHSLRQFY SC+I GN
Sbjct: 356 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGN 415

Query: 370 VDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEE 429
           VDFIFGNSAS+F +C IL+RP QL P+ G+ NA+TAHGRTDPAQSTGFVFQNCLINGT+E
Sbjct: 416 VDFIFGNSASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQE 475

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFE 489
           +M+LYY+ PQ H+ FLGRPWKEYSRTVFIHC LEALVS SGWM WSGD  L TL+YGEFE
Sbjct: 476 YMSLYYSKPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFE 535

Query: 490 NFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           N G GS +S+RV WS+QIPA++VFSYSV+NFIQG+EWIPSS
Sbjct: 536 NSGAGSKTSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSS 568

BLAST of Cucsa.249950 vs. TrEMBL
Match: A0A061DIW1_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_001430 PE=4 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.1e-202
Identity = 342/506 (67.59%), Postives = 417/506 (82.41%), Query Frame = 1

Query: 26  IAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILDSAE 85
           I  AC +TRFP  C +S++QSS LP NPTSLQIIQ+A+ +SS+NLKT KSMVK+ILDS++
Sbjct: 78  IHLACQSTRFPQPCETSLTQSSSLPPNPTSLQIIQSAIAVSSENLKTGKSMVKSILDSSK 137

Query: 86  KIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKY 145
              N NRT  A +C++ L YS+YRI+ +ND +  G++KDARAW+SA+L YQ  C S LKY
Sbjct: 138 G--NLNRTNAATTCLEILSYSDYRINSANDGLTRGKVKDARAWMSAALCYQYDCWSALKY 197

Query: 146 VNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGFWERSSGTGE 205
           VNDT+ VDQTM FL +LT  SSNAL+M+V+YDN+GN+ A+W PP+TERDGF+E  SG  +
Sbjct: 198 VNDTKLVDQTMAFLSSLTEHSSNALAMMVSYDNYGNDIASWAPPKTERDGFYESGSGGTD 257

Query: 206 VVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAP--GESAVRFVIHIKEGVYEESVR 265
           +      P + + +VTVCKDGS GCY+TVQ AV+AAP   E+  RFVIHIKEGVYEE+VR
Sbjct: 258 LGFKGGFPPELQPDVTVCKDGSGGCYKTVQDAVNAAPHNAETTRRFVIHIKEGVYEETVR 317

Query: 266 VGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPD 325
           V  EKKN+VFLGDGMGKT+ITGS NVGQPG++TYNSATVGVLGDGF+AS LTI+NTAGPD
Sbjct: 318 VPFEKKNVVFLGDGMGKTIITGSANVGQPGMTTYNSATVGVLGDGFMASGLTIKNTAGPD 377

Query: 326 AHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNC 385
           AHQAVAFRSDSDLS+I+NCEFI NQDTLY HSLRQFY  CRI GNVDFIFGNSAS+F +C
Sbjct: 378 AHQAVAFRSDSDLSVIENCEFIGNQDTLYVHSLRQFYKKCRIQGNVDFIFGNSASVFQDC 437

Query: 386 HILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTF 445
            +L+ P QL P+ G+ NAITAHGRTDPAQSTG VFQNCL+NGT+E+M  YY+ P+ HR F
Sbjct: 438 IVLVAPRQLKPEKGENNAITAHGRTDPAQSTGLVFQNCLLNGTDEYMGYYYSKPKVHRNF 497

Query: 446 LGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWS 505
           LGRPWKEYSRTVFI+C +EAL++  GW+ WSGD+GL TL+YGEF N G GSN + RV WS
Sbjct: 498 LGRPWKEYSRTVFINCFMEALITPDGWLPWSGDVGLKTLFYGEFGNSGPGSNVASRVPWS 557

Query: 506 NQIPAEYVFSYSVQNFIQGNEWIPSS 530
           +QIPA+ V++YSVQNFIQG++WIP+S
Sbjct: 558 SQIPAQRVYTYSVQNFIQGDQWIPTS 581

BLAST of Cucsa.249950 vs. TrEMBL
Match: B9RU20_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_1460660 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 1.1e-198
Identity = 342/511 (66.93%), Postives = 413/511 (80.82%), Query Frame = 1

Query: 24  PDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILDS 83
           P+I  AC ATRFPD CASS++ S  +P+NPT LQIIQ+++ +S+ NLKTA+SMVK+ILDS
Sbjct: 44  PEILQACKATRFPDTCASSLTSSDSVPKNPTPLQIIQSSVSISATNLKTAQSMVKSILDS 103

Query: 84  AEKIPNRNRTEKALSCMDALGYSEYRISLSND-AIPLGRIKDARAWLSASLLYQTGCRSG 143
           +    N NRT  A +C++ L  SEYRIS + D ++P GR KDARAW+SA+LLYQ  C S 
Sbjct: 104 SAG--NINRTNAARNCLEVLNNSEYRISSTADNSLPRGRTKDARAWMSAALLYQYDCWSA 163

Query: 144 LKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGFWERS-- 203
           LKY NDT + ++TM FLD+L +L+SN+LSMV++YD +GN+T  W PP+TERDGFWE S  
Sbjct: 164 LKYANDTSDTNKTMSFLDSLMLLTSNSLSMVMSYDIYGNDTKLWTPPKTERDGFWEGSEW 223

Query: 204 -SGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAP-GESAVRFVIHIKEGVY 263
            SG G V RGV  P+K  A+VTVCKDGS GCY+TVQ AV+ AP  E   RFV+HIKEGVY
Sbjct: 224 GSGGGLVFRGV-FPSKLTADVTVCKDGSNGCYKTVQEAVNTAPDNEMGRRFVVHIKEGVY 283

Query: 264 EESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELTIQN 323
            E VR+ +EKKN+VFLGDGMGKTVITGSL  GQPG+STYN+ATVGVLGDGF+AS LT QN
Sbjct: 284 NEIVRIPLEKKNVVFLGDGMGKTVITGSLTAGQPGVSTYNTATVGVLGDGFMASGLTFQN 343

Query: 324 TAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNSAS 383
           TAG   HQAVAFRSDSDLS I+NCEFI +QDTLYAHSLRQFY SCRI GNVDFIFGNSA+
Sbjct: 344 TAGAPTHQAVAFRSDSDLSFIENCEFIGHQDTLYAHSLRQFYKSCRIEGNVDFIFGNSAA 403

Query: 384 MFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYYANPQ 443
           +F +C I++ P Q  P+ G+ NA+TAHGRTDPAQ+TGFVFQNCLINGTEE+MAL+++ P 
Sbjct: 404 IFQDCEIVVNPRQEKPEKGENNAVTAHGRTDPAQATGFVFQNCLINGTEEYMALFHSKPG 463

Query: 444 RHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGSNSSK 503
            H+ +LGRPWKEYSR VFIHC  EA+++  GWM W+GD  L TLYYGEFEN G GSN S 
Sbjct: 464 AHKNYLGRPWKEYSRVVFIHCNFEAIITPEGWMPWTGDFALKTLYYGEFENSGPGSNLSG 523

Query: 504 RVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           RV WS+QIPAE+V++YSVQNFIQG+EWIP+S
Sbjct: 524 RVKWSSQIPAEHVYTYSVQNFIQGDEWIPTS 551

BLAST of Cucsa.249950 vs. TrEMBL
Match: G7LD86_MEDTR (Pectinesterase OS=Medicago truncatula GN=MTR_8g093460 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-195
Identity = 344/542 (63.47%), Postives = 417/542 (76.94%), Query Frame = 1

Query: 6   FLFFLPLHFSSAAHQ----------------TLLPDIAAACNATRFPDQCASSISQSSRL 65
           F  FL +  SSA H                 T   +I  AC ATRFP QC SS++    L
Sbjct: 7   FSLFLFITLSSATHHHNHNHHQPTPSPSVSPTAGSEIQQACKATRFPQQCESSLAN---L 66

Query: 66  PQNPTSLQIIQAALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYR 125
           P NPT+LQ+IQ+A+ LSS NL TA+SMVKAILDS+    +RNRT  A +C++ L  S+ R
Sbjct: 67  PPNPTALQLIQSAINLSSTNLVTAQSMVKAILDSSSS--SRNRTVAATTCIEILTNSQNR 126

Query: 126 ISLSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNA 185
           ISLSNDA+  G+IKDARAWL+A+L+YQ  C + LKY NDT  V + M F+D+L  L+SNA
Sbjct: 127 ISLSNDALTHGKIKDARAWLTAALVYQYDCWNSLKYANDTHAVGEAMSFIDSLETLTSNA 186

Query: 186 LSMVVAYDNFGNETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAG 245
           L+M  +YD +G +T+ W+PP TERDG W+ +   G  V  V IP K   +VTVCK G  G
Sbjct: 187 LAMAFSYDVYGKDTSFWKPPTTERDGLWQATGSGGGSVSSVGIPAKLTPDVTVCKGGEKG 246

Query: 246 CYRTVQAAVDAAP--GESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSL 305
           CY+TVQ AV+AAP  G    RFVI+IKEGVYEE+VRV +EK+N+VFLGDG+GKTVITGS 
Sbjct: 247 CYKTVQEAVNAAPDNGVDRKRFVIYIKEGVYEETVRVPLEKRNVVFLGDGIGKTVITGSA 306

Query: 306 NVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISN 365
           NVGQPG++TYNSATV VLGDGF+A +LTI+NTAGPDAHQAVAFR DSDLS+I+NCEF+ N
Sbjct: 307 NVGQPGMTTYNSATVAVLGDGFMAKDLTIENTAGPDAHQAVAFRLDSDLSVIENCEFLGN 366

Query: 366 QDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGR 425
           QDTLYAHSLRQFY SCRI+GNVDFIFGNSA++F +C IL+RP QL P+ G+ NAITAHGR
Sbjct: 367 QDTLYAHSLRQFYKSCRIVGNVDFIFGNSAAIFQDCQILVRPRQLKPEKGENNAITAHGR 426

Query: 426 TDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSS 485
           TDPAQSTGFVFQNCLINGTE++MALY++NP+ H+ +LGRPWKEYSRTVFIH  LE LV+ 
Sbjct: 427 TDPAQSTGFVFQNCLINGTEDYMALYHSNPKVHKNYLGRPWKEYSRTVFIHSILEVLVTP 486

Query: 486 SGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIP 530
            GWM WSGD  L TLYYGEFEN G GS+ S+RV+WS++IPAE+V SYS +NFIQG EW+ 
Sbjct: 487 QGWMPWSGDFALKTLYYGEFENSGAGSDLSQRVSWSSKIPAEHVSSYSAENFIQGGEWMQ 543

BLAST of Cucsa.249950 vs. TAIR10
Match: AT5G09760.1 (AT5G09760.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 642.1 bits (1655), Expect = 3.0e-184
Identity = 324/543 (59.67%), Postives = 406/543 (74.77%), Query Frame = 1

Query: 5   LFLFFLPLHFSSAAHQ-------------TLLPDIAAACNATRFPDQCASSISQSSRLPQ 64
           LFLF  P   S   H              +L   I  ACNATR+PDQC SS+S+  R+P 
Sbjct: 11  LFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSLSEQGRVPP 70

Query: 65  NPTSLQIIQAALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRIS 124
           +P  +QII +A+  S  NLKTA+S +K+I+DS+  + N NRT  A +C+  L YSE+R  
Sbjct: 71  DPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSS--VGNLNRTNAANTCLQLLTYSEHRTQ 130

Query: 125 LSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALS 184
            ++ A+  G+IKDARAW+SA+L+YQ    S LKYVNDT +V +TM FLD L  ++SNALS
Sbjct: 131 STDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHVTSNALS 190

Query: 185 MVVAYDNFGNETAAWRPPRTERDGFWERSS-GTG-EVVRGVRI--PTKTEANVTVCKDGS 244
           M+V+YDNFG+  A+W  P TERDGFWE++  G G +   G+ +  P+  + +VTVCKDG 
Sbjct: 191 MMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVTVCKDGK 250

Query: 245 AGCYRTVQAAVDAAPGESAVR-FVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGS 304
            G Y+TVQ AV+AAP ++ +R FVI I EGVYEE+V V  EKKN+VF+GDGMGKTVITGS
Sbjct: 251 CG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGKTVITGS 310

Query: 305 LNVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFIS 364
           LN G PGI+TYN+ATVGV+GDGF+A +LT QNTAGPDAHQAVAFRSDSD S+I+NCEF+ 
Sbjct: 311 LNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSLIENCEFLG 370

Query: 365 NQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHG 424
           NQDTLYAH LRQFY +CRI GNVDFIFGNSA++F +C ILI P Q+NP+ G++NA+TA G
Sbjct: 371 NQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGEKNAVTAQG 430

Query: 425 RTDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVS 484
           R DP+QSTGFVF NCLINGTEE+M L+ ANP+ H+ FLGRPWK+YSRTVFI C LEAL++
Sbjct: 431 RIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIGCNLEALIT 490

Query: 485 SSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWI 530
             GW+ WSGD  L TLYYGE +N G GS+ S+RV+WS+QIP E+V  YSV NFIQ +EW 
Sbjct: 491 PDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVANFIQADEWA 550

BLAST of Cucsa.249950 vs. TAIR10
Match: AT5G64640.1 (AT5G64640.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 604.4 bits (1557), Expect = 6.9e-173
Identity = 300/511 (58.71%), Postives = 385/511 (75.34%), Query Frame = 1

Query: 23  LPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILD 82
           +  I  ACNATRFPD C +S+S+  ++P +P  +QII +A+ +S +NLK+ +S +++ILD
Sbjct: 90  IAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILD 149

Query: 83  SAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSG 142
           S+    NRNRT  A  C++ L YS++R   ++ A+  G IKDARAW+SA+L YQ  C SG
Sbjct: 150 SSAG--NRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSG 209

Query: 143 LKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGFWERS-- 202
           LK VNDT++V  T+ F + L  L+ NALSM++++D+FG++  +W  P TERDGFWE++  
Sbjct: 210 LKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGP 269

Query: 203 ---SGTGEVVRGVRIPTKTEANVTVCKDGSAGC-YRTVQAAVDAAPGES-AVRFVIHIKE 262
              SGTG     +  P+    +VTVCK+G   C Y+TVQ AVD+AP  +  V+FVI I+E
Sbjct: 270 SLGSGTGTEA-SLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIRE 329

Query: 263 GVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELT 322
           GVYEE+VRV  EKKN+VF+GDGMGKTVITGSLNVGQPG++T+ SATVGVLGDGF+A +LT
Sbjct: 330 GVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLT 389

Query: 323 IQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGN 382
           I+NTAG DAHQAVAFRSDSD S+++NCEF+ NQDTLYAHSLRQFY  CRI GNVDFIFGN
Sbjct: 390 IENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGN 449

Query: 383 SASMFLNCHILI-RPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYY 442
           SA++F +C ILI   H    Q G  NAITAHGR D +QSTGFVF NC INGTEE+M  + 
Sbjct: 450 SAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQ 509

Query: 443 ANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGS 502
           ANP+ H+ FLGRPWKE+SRTVF++C LE+L+S  GWM W+GD  L TLYYGE++N G GS
Sbjct: 510 ANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGS 569

Query: 503 NSSKRVAWSNQIPAEYVFSYSVQNFIQGNEW 526
             S RV WS++IP ++V  YSV NFIQ +EW
Sbjct: 570 VRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

BLAST of Cucsa.249950 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 300.1 bits (767), Expect = 2.8e-81
Identity = 188/536 (35.07%), Postives = 280/536 (52.24%), Query Frame = 1

Query: 20  QTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLS----SDNLK---- 79
           +T    + A C  T + + C +S+ ++S  P +   L +I+    ++     D++K    
Sbjct: 49  KTATTAVEAVCAPTDYKETCVNSLMKAS--PDSTQPLDLIKLGFNVTIRSIEDSIKKASV 108

Query: 80  --TAKSM----VKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDA 139
             TAK+      K  L+  EK+ N + T+    C+D   +  + I    D +     +D 
Sbjct: 109 ELTAKAANDKDTKGALELCEKLMN-DATDDLKKCLD--NFDGFSIPQIEDFV-----EDL 168

Query: 140 RAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNF------ 199
           R WLS S+ YQ  C    +  N     D   +F  +   L+SN L+M+    N       
Sbjct: 169 RVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRE-LTSNGLAMITNISNLLGEFNV 228

Query: 200 ----GNETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTK--TEANVTVCKDGSAGCYRT 259
               G+     R   +  DG     S  G   R + + TK   +ANV V  DGS G Y+T
Sbjct: 229 TGVTGDLGKYARKLLSAEDGI---PSWVGPNTRRL-MATKGGVKANVVVAHDGS-GQYKT 288

Query: 260 VQAAVDAAPGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPG 319
           +  A++A P  +   FVI+IK+GVY E V V  +  ++ F+GDG  KT ITGSLN     
Sbjct: 289 INEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGK 348

Query: 320 ISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYA 379
           + TY +ATV + GD F A  +  +NTAGP+ HQAVA R  +DL++  NC+    QDTLY 
Sbjct: 349 VKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYV 408

Query: 380 HSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQS 439
           HS RQF+  C + G VDFIFG+   +  NC+I++R     P       ITA GR+D  +S
Sbjct: 409 HSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRES 468

Query: 440 TGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAW 499
           TG V QNC I G   ++ +   N    + +LGRPWKE+SRT+ +   ++ ++  +GW+ W
Sbjct: 469 TGLVLQNCHITGEPAYIPVKSIN----KAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPW 528

Query: 500 SGDIGLNTLYYGEFENFGTGSNSSKRVAWS--NQIPAEYVFSYSVQNFIQGNEWIP 528
           +GD  LNTLYY E+EN G GSN ++RV W    ++  +    ++   F++GN WIP
Sbjct: 529 NGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIP 560

BLAST of Cucsa.249950 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 297.4 bits (760), Expect = 1.8e-80
Identity = 181/523 (34.61%), Postives = 280/523 (53.54%), Query Frame = 1

Query: 21  TLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSL--QIIQAALYLSSDNLKTAKSMVK 80
           T +  I   C  T + + C  ++ + ++   +P  L      A +   SD  K +++M++
Sbjct: 52  TSVKAIKDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIE 111

Query: 81  AILDSAEKIPNRNRTEKALS-CMDALGYSEYRISLSNDAI---PLGRIKDA----RAWLS 140
              D         R + AL  C + + Y+   +S S + +      ++ +A    R WLS
Sbjct: 112 LQKDP--------RAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLS 171

Query: 141 ASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPR 200
           A++ ++  C  G +        +     L     L+ N L+MV    N+  +      P 
Sbjct: 172 ATISHEQTCLDGFQGTQGNAG-ETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQI---PE 231

Query: 201 TERDGFWERSSGTGEVVRGVRI----PTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESA 260
                   +   +    R  R+     ++ + ++ V +DGS G Y+T+  A++  P +  
Sbjct: 232 MNSRRLLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGS-GQYKTINEALNFVPKKKN 291

Query: 261 VRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLG 320
             FV+HIKEG+Y+E V+V     +LVF+GDG  KTVI+GS +  + GI+TY +ATV ++G
Sbjct: 292 TTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSY-KDGITTYKTATVAIVG 351

Query: 321 DGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRIL 380
           D FIA  +  +NTAG   HQAVA R  +D SI  NC+F   QDTLYAHS RQFY  C I 
Sbjct: 352 DHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTIS 411

Query: 381 GNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGT 440
           G +DF+FG++A++F NC +L+R     P L     ITAHGR DP +STGFV Q C I G 
Sbjct: 412 GTIDFLFGDAAAVFQNCTLLVR----KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGE 471

Query: 441 EEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGE 500
            +++A+     ++ +T+LGRPWKEYSRT+ ++  +   V   GW  W G+ GLNTL+Y E
Sbjct: 472 PDYLAV----KEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSE 531

Query: 501 FENFGTGSNSSKRVAWS--NQIPAEYVFSYSVQNFIQGNEWIP 528
            +N G G+  +KRV W    ++  E +  ++   +IQG+ WIP
Sbjct: 532 VQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIP 552

BLAST of Cucsa.249950 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 295.0 bits (754), Expect = 8.9e-80
Identity = 192/546 (35.16%), Postives = 290/546 (53.11%), Query Frame = 1

Query: 15  SSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAK 74
           SS +H  L     + C++T +P+ C S+++ +    +  +  ++I+A+L L++  +K   
Sbjct: 55  SSTSHAIL----KSVCSSTLYPELCFSAVAATGG-KELTSQKEVIEASLNLTTKAVKHNY 114

Query: 75  SMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPL--------GRIKDAR 134
             VK ++   + +  R  T     C++ +  +   + ++ + +              D +
Sbjct: 115 FAVKKLIAKRKGLTPREVTALH-DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLK 174

Query: 135 AWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLD-NLTVLSSNALSMVV----------- 194
             +S+++  Q  C  G  Y +  R+V + +L    ++  + SNAL+M+            
Sbjct: 175 TLISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFE 234

Query: 195 ------AYDNFGNETAAWRPPRTERDGFWER--SSGTGEVVRGVRIPTKTEANVTVCKDG 254
                  + N  N          + DG W +  S G   +++G  I    +A+ TV  DG
Sbjct: 235 LRDKSSTFTNNNNRKLKEVTGDLDSDG-WPKWLSVGDRRLLQGSTI----KADATVADDG 294

Query: 255 SAGCYRTVQAAVDAAPGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGS 314
           S G + TV AAV AAP +S  RFVIHIK GVY E+V V  +K N++FLGDG GKT+ITGS
Sbjct: 295 S-GDFTTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGS 354

Query: 315 LNVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFIS 374
            NV   G +T++SATV  +G+ F+A ++T QNTAGP  HQAVA R  SD S    C+  +
Sbjct: 355 RNVVD-GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFA 414

Query: 375 NQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHG 434
            QDTLY HS RQF+  C I G VDFIFGN+A++  +C I  R     P  G +N +TA G
Sbjct: 415 YQDTLYVHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINAR----RPNSGQKNMVTAQG 474

Query: 435 RTDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVS 494
           R+DP Q+TG V QNC I GT + +A+    P    T+LGRPWKEYSRTV +  ++  ++ 
Sbjct: 475 RSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP----TYLGRPWKEYSRTVIMQSDISDVIR 534

Query: 495 SSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWSNQ---IPAEYVFSYSVQNFIQGN 530
             GW  WSG   L+TL Y E+ N G G+ ++ RV W              ++   FI G 
Sbjct: 535 PEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGG 579

BLAST of Cucsa.249950 vs. NCBI nr
Match: gi|449459280|ref|XP_004147374.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus])

HSP 1 Score: 1065.8 bits (2755), Expect = 2.4e-308
Identity = 528/529 (99.81%), Postives = 528/529 (99.81%), Query Frame = 1

Query: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60
           MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ
Sbjct: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60

Query: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDA 240
           NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGS GCYRTVQAAVDA
Sbjct: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSEGCYRTVQAAVDA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420

Query: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480
           CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN
Sbjct: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 529

BLAST of Cucsa.249950 vs. NCBI nr
Match: gi|659121956|ref|XP_008460901.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 1008.4 bits (2606), Expect = 4.5e-291
Identity = 497/529 (93.95%), Postives = 508/529 (96.03%), Query Frame = 1

Query: 1   MPSLLFLFFLPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQ 60
           MPSLL LFFLPLHFSSAAHQTL P+IAAACN+TRFPDQCASSISQS+RL QNPT LQII 
Sbjct: 1   MPSLLLLFFLPLHFSSAAHQTLPPEIAAACNSTRFPDQCASSISQSNRLLQNPTPLQIIH 60

Query: 61  AALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLG 120
           AALYLSS+NLKTAKSMVKAILDSAEKIPNRNRT  ALSCMDALGYSEYRISLSNDAIPLG
Sbjct: 61  AALYLSSENLKTAKSMVKAILDSAEKIPNRNRTAAALSCMDALGYSEYRISLSNDAIPLG 120

Query: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFG 180
           RIKDARAWLSASLLYQTGCRSGLKYVNDT EVDQTM FLDNLTVLSSNALSMVVAYDNFG
Sbjct: 121 RIKDARAWLSASLLYQTGCRSGLKYVNDTHEVDQTMSFLDNLTVLSSNALSMVVAYDNFG 180

Query: 181 NETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDA 240
           NETAAW PPRTERDGFWE SSGTGEVVRGVRIP KTE NVTVCKDGS GCYRTVQAAV+A
Sbjct: 181 NETAAWLPPRTERDGFWEHSSGTGEVVRGVRIPAKTEVNVTVCKDGSEGCYRTVQAAVEA 240

Query: 241 APGESAVRFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSA 300
           APGESAVRFVIHIKEGVYEESVRVG+EKKNLVFLGDGMGKTVITGSL+VGQPGISTYNSA
Sbjct: 241 APGESAVRFVIHIKEGVYEESVRVGIEKKNLVFLGDGMGKTVITGSLSVGQPGISTYNSA 300

Query: 301 TVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFY 360
           TVGVLGDGFIASELTIQNTAGPD HQAVAFRSDSDLSII NCEFISNQDTLYAHSLRQFY
Sbjct: 301 TVGVLGDGFIASELTIQNTAGPDVHQAVAFRSDSDLSIIHNCEFISNQDTLYAHSLRQFY 360

Query: 361 NSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQN 420
           NSCRILGNVDFIFGNSAS FLNCHIL+RPH LNPQLGDEN ITAHGRTDPAQSTGFVFQN
Sbjct: 361 NSCRILGNVDFIFGNSASFFLNCHILVRPHPLNPQLGDENPITAHGRTDPAQSTGFVFQN 420

Query: 421 CLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLN 480
           CL+NGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWM WSGDIGLN
Sbjct: 421 CLLNGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMPWSGDIGLN 480

Query: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           TLYYGEFENFGTGSNSSKRVAWSNQIPAE+ FSYS+QNFIQGNEWIPSS
Sbjct: 481 TLYYGEFENFGTGSNSSKRVAWSNQIPAEHAFSYSIQNFIQGNEWIPSS 529

BLAST of Cucsa.249950 vs. NCBI nr
Match: gi|659121958|ref|XP_008460902.1| (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 743.0 bits (1917), Expect = 3.5e-211
Identity = 369/521 (70.83%), Postives = 423/521 (81.19%), Query Frame = 1

Query: 10  LPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDN 69
           LP HF++     +L     AC +TRFPDQC SS+S SS  P NP+ LQI+Q+A+ LSSD 
Sbjct: 57  LPSHFTNTPSDQILH----ACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDG 116

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L TA+S+VK ILDS+    N NRT  A +C++ L  S+YRISL+NDA+  GR KDARAWL
Sbjct: 117 LTTAQSLVKQILDSSAT--NLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWL 176

Query: 130 SASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPP 189
           SASLLY   C S LKY NDT+ VD+TM FLD+L  LSSNALSM+VAYD +GN+TAAW PP
Sbjct: 177 SASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPP 236

Query: 190 RTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESAVR- 249
           RTERDGFWE   G   +      P K  A+ TVCKDGS GCY ++Q AVDAAP  +A R 
Sbjct: 237 RTERDGFWEGGGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARR 296

Query: 250 FVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVYEE VRV +EKKN+VFLGDGMGKTVITGSLN GQPGISTYN+AT+GVLGDG
Sbjct: 297 FVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDG 356

Query: 310 FIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPDAHQAVAFRSDSDLS+I +CEFI NQDTLYAHSLRQFY SC+I GN
Sbjct: 357 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGN 416

Query: 370 VDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEE 429
           VDFIFGNSAS+F +C ILIRP QL P+ G+ NAITAHGRTDPAQSTGFVFQNCLINGT+E
Sbjct: 417 VDFIFGNSASIFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQE 476

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFE 489
           +M+LYY+ P+ H+ FLGRPWKEYSRTVFIHC LEALVS SGWM WSGD  L TL+YGEFE
Sbjct: 477 YMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFE 536

Query: 490 NFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           N G GS +S+RV WS+QIPA++VFSYSVQNFIQG+EWIPSS
Sbjct: 537 NSGVGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSS 571

BLAST of Cucsa.249950 vs. NCBI nr
Match: gi|778670202|ref|XP_004147375.2| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus])

HSP 1 Score: 740.3 bits (1910), Expect = 2.3e-210
Identity = 368/521 (70.63%), Postives = 427/521 (81.96%), Query Frame = 1

Query: 10  LPLHFSSAAHQTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDN 69
           LP HF++     +L     AC +TRFPDQC SS+S SS LP NP+ LQI+Q+A+ LSSD 
Sbjct: 56  LPSHFTNYPSDQILH----ACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDG 115

Query: 70  LKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWL 129
           L TA+S+VK ILDS+    N NRT  A +C++ L  S+YRISL+NDA+  GR KDARAWL
Sbjct: 116 LTTAQSLVKQILDSSAT--NLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWL 175

Query: 130 SASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPP 189
           SASLLY   C S LKY NDT+ VD+TM FLD+L  LSSNALSM+VAYD +GN+TAAW PP
Sbjct: 176 SASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPP 235

Query: 190 RTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAPGESAVR- 249
           RTERDGFWE   G+G    G   P K  A+ TVCKDGS GCY ++Q AVDAAP  +A R 
Sbjct: 236 RTERDGFWEGGGGSGLGFSG-GFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARR 295

Query: 250 FVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDG 309
           FVIHIKEGVYEE VRV +EKKN+VFLGDGMGKTVITGSLNVGQPG+STYN+AT+GV+GDG
Sbjct: 296 FVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDG 355

Query: 310 FIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGN 369
           F+AS LTIQNTAGPDAHQAVAFRSDSDLS+I +CEF+ NQDTLYAHSLRQFY SC+I GN
Sbjct: 356 FMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGN 415

Query: 370 VDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEE 429
           VDFIFGNSAS+F +C IL+RP QL P+ G+ NA+TAHGRTDPAQSTGFVFQNCLINGT+E
Sbjct: 416 VDFIFGNSASIFQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQE 475

Query: 430 FMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFE 489
           +M+LYY+ PQ H+ FLGRPWKEYSRTVFIHC LEALVS SGWM WSGD  L TL+YGEFE
Sbjct: 476 YMSLYYSKPQVHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFE 535

Query: 490 NFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 530
           N G GS +S+RV WS+QIPA++VFSYSV+NFIQG+EWIPSS
Sbjct: 536 NSGAGSKTSERVGWSSQIPAQHVFSYSVENFIQGDEWIPSS 568

BLAST of Cucsa.249950 vs. NCBI nr
Match: gi|590708612|ref|XP_007048328.1| (Plant invertase/pectin methylesterase inhibitor superfamily [Theobroma cacao])

HSP 1 Score: 713.4 bits (1840), Expect = 3.0e-202
Identity = 342/506 (67.59%), Postives = 417/506 (82.41%), Query Frame = 1

Query: 26  IAAACNATRFPDQCASSISQSSRLPQNPTSLQIIQAALYLSSDNLKTAKSMVKAILDSAE 85
           I  AC +TRFP  C +S++QSS LP NPTSLQIIQ+A+ +SS+NLKT KSMVK+ILDS++
Sbjct: 78  IHLACQSTRFPQPCETSLTQSSSLPPNPTSLQIIQSAIAVSSENLKTGKSMVKSILDSSK 137

Query: 86  KIPNRNRTEKALSCMDALGYSEYRISLSNDAIPLGRIKDARAWLSASLLYQTGCRSGLKY 145
              N NRT  A +C++ L YS+YRI+ +ND +  G++KDARAW+SA+L YQ  C S LKY
Sbjct: 138 G--NLNRTNAATTCLEILSYSDYRINSANDGLTRGKVKDARAWMSAALCYQYDCWSALKY 197

Query: 146 VNDTREVDQTMLFLDNLTVLSSNALSMVVAYDNFGNETAAWRPPRTERDGFWERSSGTGE 205
           VNDT+ VDQTM FL +LT  SSNAL+M+V+YDN+GN+ A+W PP+TERDGF+E  SG  +
Sbjct: 198 VNDTKLVDQTMAFLSSLTEHSSNALAMMVSYDNYGNDIASWAPPKTERDGFYESGSGGTD 257

Query: 206 VVRGVRIPTKTEANVTVCKDGSAGCYRTVQAAVDAAP--GESAVRFVIHIKEGVYEESVR 265
           +      P + + +VTVCKDGS GCY+TVQ AV+AAP   E+  RFVIHIKEGVYEE+VR
Sbjct: 258 LGFKGGFPPELQPDVTVCKDGSGGCYKTVQDAVNAAPHNAETTRRFVIHIKEGVYEETVR 317

Query: 266 VGMEKKNLVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVLGDGFIASELTIQNTAGPD 325
           V  EKKN+VFLGDGMGKT+ITGS NVGQPG++TYNSATVGVLGDGF+AS LTI+NTAGPD
Sbjct: 318 VPFEKKNVVFLGDGMGKTIITGSANVGQPGMTTYNSATVGVLGDGFMASGLTIKNTAGPD 377

Query: 326 AHQAVAFRSDSDLSIIDNCEFISNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASMFLNC 385
           AHQAVAFRSDSDLS+I+NCEFI NQDTLY HSLRQFY  CRI GNVDFIFGNSAS+F +C
Sbjct: 378 AHQAVAFRSDSDLSVIENCEFIGNQDTLYVHSLRQFYKKCRIQGNVDFIFGNSASVFQDC 437

Query: 386 HILIRPHQLNPQLGDENAITAHGRTDPAQSTGFVFQNCLINGTEEFMALYYANPQRHRTF 445
            +L+ P QL P+ G+ NAITAHGRTDPAQSTG VFQNCL+NGT+E+M  YY+ P+ HR F
Sbjct: 438 IVLVAPRQLKPEKGENNAITAHGRTDPAQSTGLVFQNCLLNGTDEYMGYYYSKPKVHRNF 497

Query: 446 LGRPWKEYSRTVFIHCELEALVSSSGWMAWSGDIGLNTLYYGEFENFGTGSNSSKRVAWS 505
           LGRPWKEYSRTVFI+C +EAL++  GW+ WSGD+GL TL+YGEF N G GSN + RV WS
Sbjct: 498 LGRPWKEYSRTVFINCFMEALITPDGWLPWSGDVGLKTLFYGEFGNSGPGSNVASRVPWS 557

Query: 506 NQIPAEYVFSYSVQNFIQGNEWIPSS 530
           +QIPA+ V++YSVQNFIQG++WIP+S
Sbjct: 558 SQIPAQRVYTYSVQNFIQGDQWIPTS 581