Carg12291 (gene) Silver-seed gourd

NameCarg12291
Typegene
OrganismCucurbita argyrosperma (Silver-seed gourd)
Description4-coumarate-CoA ligase
LocationCucurbita_argyrosperma_scaffold_074 : 103636 .. 106961 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTCACGATTTCAGAATCTAAAAAATTTAAATCTCCTTCGCTTTCCGAGTCAAGGTTGAAGACGAAGACCCAAATTCTACGCACTTCCAGCCATTAATGGTGGCCATTTTTTCTTCCTACCTAATGCAGCAAACAACCATACACTGAGGTGGTCTCCTCACTCTTAATAGAAGTCTACTTACGTTTTTTCCGTTGCGAAGCAATTGAATCTGTTTTATCGTATCTTCTAAATTGCTTGTCTTCTTTTGTACTCGGACTCTTACGTTCTCTATTTAAATATTGATAGTAAATTTCCCCTCCAATCCGCTTCGGGTTGTGTGCAACTTCTTTATCTGAAACCTCTTTGCAATTGCGTCTGTGATTTTGATTCCTTCAATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGTACCCTGTAGTAGGCTGTTTGTGTTCTATAATTATGTTTTACTATAATCTATTGGATGCTTTTTCATTCTTTCACTTTCTTATATCAAGAACATCAAATGATATTTTCACTTAGAACCAACAGAGTTTGAAATGCAGAGGAGTTGGGACAATTAGAACTTAGAACAGTAGTGAAACACTTTTCTTTTAAATGCCTCCAAAAAGTACTTTTATGTATGTGTTAGAGATGACCTGTGACTTTTGAAGATAGAAAAACACTATTTAAGTAATCTTAGCAATTTCAAAAGAAGTTTTAACTAGGTTACCGAACACTGGTAAAATCAGCTTTCGTCGTTACAAAAGACATACCGACCCTAAGTGTTTATAGAATTCTCAAAATCTATGGTCATGCAATAAAAACCATGAAATTTTTAAAAGTTACTTCATTTGGTTAAAAATGCTTTTCCTATTCTAAAAGTCATATACATTCATCCTGACTATCGGCAACTCTAAATTCTGAATGGAACTTCTAAATGTTTTCACTTCATTTGGCTTTGATTTATAACCTTGTAGAGGTGGATTATAAGATTATAATGCAATAACTTGAATTCATTGCTATTCTCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTATATAGATATGGTTATCTTTCTCTTGATTTATGGTTTTCATACAATACTTTGGAGAGAATGTTTTTGTTTCGTAATCGAATACGATCTGAGTTAATGTGATCTTTAGTGTGTTGGGAGTTACTATTAGTACCTAACATTCAAGTCGGTTTTCGAATTCAATTTTGAAATGATGTGCAGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTACATATCTGTTTATCTAGTAATATTTAGCTCTAATCTCAGCAGTTTTAGCTTTCAGCTTTTGGAACTATGTCGAAACATTAGCATCCAGTAACTTCTTACCAATGATATCGTGCTATATCGGTCGTCCTTGTACGAGCAAGTGCTGTTTTGAGTTCAAATCAAATTCTTAAGCTGTCCTCCTCTCTCAAATTTTGTTACTGAATCTCCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCGTAAGCGTTCATGAAACTCCTTACTCGTGTTTGAACTTTCGACATTCACTCCTTTGTAACTGATTCGATGAAAACCGAATCAACAGCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTAAGGCACTTAAGAAGAAAGTTCGGTTAATTTCAGCAGCACGGATAGATTGCTCGATATGCATCAGGAATGAAACTTGTGTGTTTCTCCTGCAACAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAAGTTCTTTGGTTCCTCTTTTCATTACGACACGAGCAGAATAAGACCTTTTTGTTCTCTTATTTTCACACTTTTATGTAATTTCTTTTTAATATAACTAACTATGTAGTTCAAGTTTCTATAATAATTTACTTTT

mRNA sequence

TTTTTTTTTCACGATTTCAGAATCTAAAAAATTTAAATCTCCTTCGCTTTCCGAGTCAAGGTTGAAGACGAAGACCCAAATTCTACGCACTTCCAGCCATTAATGGTGGCCATTTTTTCTTCCTACCTAATGCAGCAAACAACCATACACTGAGGTGGTCTCCTCACTCTTAATAGAAGTCTACTTACGTTTTTTCCGTTGCGAAGCAATTGAATCTGTTTTATCGTATCTTCTAAATTGCTTGTCTTCTTTTGTACTCGGACTCTTACGTTCTCTATTTAAATATTGATAGTAAATTTCCCCTCCAATCCGCTTCGGGTTGTGTGCAACTTCTTTATCTGAAACCTCTTTGCAATTGCGTCTGTGATTTTGATTCCTTCAATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAAGTTCTTTGGTTCCTCTTTTCATTACGACACGAGCAGAATAAGACCTTTTTGTTCTCTTATTTTCACACTTTTATGTAATTTCTTTTTAATATAACTAACTATGTAGTTCAAGTTTCTATAATAATTTACTTTT

Coding sequence (CDS)

ATGGAGAAATCTGGATATGGTCGCGATGGAATTTTCAGGTCCTTACGGCCTCCGATCGTCTTTCCCAGAGATCCCAATTTGTCGGTGGCCTCTTTTCTTTTCCGAAACTCATCGTCTTATCCAAACAGACTCGCCATAGTTGATGCGGAATCTTCTGATTCCGTGTCGTATTCCCAGCTTAAAGCCTCTGCAATTAAGATCTCCAATGGTTTGCTCCAATTGGGTATTGAGAAAAACGACGTGGTGATGATTTTTGCTCCAAATTCTGTTGAATTCTCCATTTGTTTCATTGGAATTGTTGCTATTGGGGCTATTGTCACCACTTGTAATCCTGTTTACATGGTCTCTGAGTTAACCAAGCAAGTTAGAGATGCTAAGCCCAAACTTATGATATCTGTTGTTGAATTGTGGGACAAAGTGAAGGATCTAAACATCCCAACAGTGTTACTAGACCAGCAAATTCCTTCGGAAATCCAGTCCTCCAAAATTTTATGTTTCAATGATTTGATAAACATGGCTGGCGATAAATCTGGCTCCGAATTCCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCTCTTCTGTATTCATCAGGAACAACAGGAGCCAGTAAGGGTGTGATTCTGACTCACAGAAACTTCATAACATCTTCACTGATGATAACATTGGATCAGAAGACTAATGGAGACGAAAATGGCACATATCTGAACTTTTTGCCGATGTTTCATGTGTTTGGATTGGTTTGTATAACATGTGCGCAGCTGCAGAGAGGAAACACCATTGTCTCTATGCCAAAGTTTAACCTGGAGAAGGCCCTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTTGTGCTTGCTTTGGCAAAGCAAAGTGTGGTAAAGAAATACAACCTTTCATCCCTGAAGCATATCGGTTCAGGGGCTGCACCTCTTGGGAAGGAATTAATGGATGAATGTGCTAACAACATTCCCAGTGCTGTGGTTCTCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTTCTTTGGAGAATCGAACAGTTGGCAAACGAAATACGGGTTCGGCTGGAACACTTGCCTCTGGTGTTGAGGCTCAAATAGTCAGCGTAGATACTTTGAAGCCTCTTCCTCCTAATCAATATGGAGAGATACTGGTTCGGGGACCGAATATGATGCTTGGTTACTTAAACAATCCACAGGCCACCAAGCAAACTATTGACAAATATGGCTGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACAGAATTAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCCGAGCTTGAAGCACTGTTGGTCTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTACCCCGACGATGAAGCTGGAGAAGTTCCAATCGCATATGTCGTGCGATCTACCAATAGTTCTATGACCGAAGAGGACGTCCTCAAATTCGTGGCCGATCAGGTTTCTCCTTACAAAAGGTTAAGAAGAGTGACATTCATCAACAGTGTCCCCAAGTCAGTTTCTGGGAAAATCCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTAA

Protein sequence

MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVRAKI
BLAST of Carg12291 vs. NCBI nr
Match: XP_022949355.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 1061.2 bits (2743), Expect = 1.2e-306
Identity = 542/543 (99.82%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AK+
Sbjct: 541 AKL 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_023525684.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.1 bits (2722), Expect = 3.2e-304
Identity = 539/543 (99.26%), Postives = 540/543 (99.45%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASF+FRNSSSYPNRLAIVDAESSDSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFIFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVK LNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKS SE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKVLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSSSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRN ITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNLITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_022998874.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 1048.1 bits (2709), Expect = 1.0e-302
Identity = 535/543 (98.53%), Postives = 538/543 (99.08%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPPIVFP DPNLSVASFLFRNSSSYPNRLAIVDAESSDS SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVY VSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKLMISVVELWDKVKDLNIPTVLLD+QIP EIQSSKILCFNDLINMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTHRNFITSSLMITLDQKTNGDENGTYLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENR VGKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_008463063.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo])

HSP 1 Score: 968.8 bits (2503), Expect = 7.9e-279
Identity = 485/543 (89.32%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSG GRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVVMIFAPNSV+ SICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVKDLNIPTVLLDQ+IPS I SSKILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFI SSLMIT+DQ  +G+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI++VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. NCBI nr
Match: XP_004147290.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothetical protein Csa_1G108800 [Cucumis sativus])

HSP 1 Score: 966.8 bits (2498), Expect = 3.0e-278
Identity = 483/543 (88.95%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-212
Identity = 362/546 (66.30%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGI+RSLRP +V P+DPN S+ SFLFRNSSSYP++LAI D+++ DS+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  ++++G  +LGI KNDVV+IFAPNS +F +CF+ + AIG + TT NP+Y V+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEI---QSSKILCFNDLINMAGDKS 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL  +   EI    +SKIL F++++ ++  + 
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELS--EP 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 240
            SE+P V +KQSDTAALLYSSGTTG SKGV LTH NFI +SLM+T+DQ   G+ +G +L 
Sbjct: 181 VSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLC 240

Query: 241 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGL  IT +QLQRGN +VSM +F LE  L  +EK++VT LWVVPPV LAL+KQ
Sbjct: 241 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 300

Query: 301 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 360
           S+VKK++LSSLK+IGSGAAPLGK+LM+EC  NIP+ +++QGYGMTETCG+VS+E+  +GK
Sbjct: 301 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGK 360

Query: 361 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 420
           RN+GSAG LA GVEAQIVSV+T K  PPNQ GEI VRGPNMM GYLNNPQATK+TIDK  
Sbjct: 361 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 480
           WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP+PD+E
Sbjct: 421 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 480

Query: 481 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 540
           AGEVPIA+VVRS NSS+TE+D+ KF+A QV+PYKRLRRV+FI+ VPKS +GKILRREL++
Sbjct: 481 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 540

Query: 541 KVRAKI 544
           +VR+K+
Sbjct: 541 QVRSKM 544

BLAST of Carg12291 vs. TAIR10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1)

HSP 1 Score: 418.7 bits (1075), Expect = 5.5e-117
Identity = 218/540 (40.37%), Postives = 350/540 (64.81%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLK 62
           +SG+   +  F S R PI  P +P+L V +F+  +S ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 122
            +   +++ L ++GI K  VV++ +PNS+ F +  + ++++GAI+TT NP+   +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLMISVVELWDKVKDL--NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGS 182
           ++D+ P L  +  +L  K+      +P VL+D++    +   +      L+ M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFL 242
                 V Q DTA LLYSSGTTG SKGVI +HRN I + +   +++  + D    ++  +
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLI-AMVQTIVNRFGSDDGEQRFICTV 246

Query: 243 PMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK--Q 302
           PMFH++GL       L  G+TI+ + KF + + + A+ KY+ T L +VPP+++A+     
Sbjct: 247 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 306

Query: 303 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 362
            +  KY+LSS+  +  G APL KE+ +  A   P+  +LQGYG+TE+ G+ +  +     
Sbjct: 307 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 366

Query: 363 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 422
           R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GY +N +AT  T+D  G
Sbjct: 367 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 426

Query: 423 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 482
           W+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP+PD E
Sbjct: 427 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKE 486

Query: 483 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 538
            G+ P+AYVVR T SS++E+ +++FVA QV+PYKR+R+V F++S+PK+ SGKILR++LI+
Sbjct: 487 VGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of Carg12291 vs. TAIR10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 402.5 bits (1033), Expect = 4.1e-112
Identity = 231/549 (42.08%), Postives = 339/549 (61.75%), Query Frame = 0

Query: 3   KSGYGRD-GIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLK 62
           KSG+     IF S R P+  P +  L V SF+   S  +  +   VDA +   +S+ +L 
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSFI--ASQPHRGKTVFVDAVTGRRLSFPELW 83

Query: 63  ASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 122
               +++  L  LG+ K +VV+I +PNS+ F I  + ++++GAI+TT NP+    E++KQ
Sbjct: 84  LGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQ 143

Query: 123 VRDAKPKLMISVVELWDKV---KDLNIPTVLLDQ-QIPSEIQSSKILCFNDLINMAGDKS 182
           + D++P L  +  +L  K+    + N+P VL+D   +PS+    ++     L  M   + 
Sbjct: 144 IGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIETEP 203

Query: 183 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 242
                   V Q DTAALLYSSGTTG SKGV+L+HRN I    ++   +   G E  T   
Sbjct: 204 SESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA---LVQAYRARFGLEQRTICT 263

Query: 243 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK- 302
            +PM H+FG        +  G TIV +PKF++ K L AVE ++ + L +VPP+V+A+   
Sbjct: 264 -IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVNG 323

Query: 303 -QSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGV-VSLENRT 362
              +  KY+LSSL  + +G APL +E+ ++   N P   +LQGYG+TE+  +  S+ N+ 
Sbjct: 324 ANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNKE 383

Query: 363 VGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTID 422
             KR  G++G LA  VE +IV  DT + L  NQ GE+ +R P +M GY  N +AT  TID
Sbjct: 384 ETKR-YGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 443

Query: 423 KYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYP 482
             GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP P
Sbjct: 444 SEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIP 503

Query: 483 DDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRE 542
           D +AG+ P+AY+VR   S+++E +++ FVA QVSPYK++R+VTF+ S+PK+ SGKILRRE
Sbjct: 504 DMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRRE 563

Query: 543 LIEKVRAKI 544
           L +   +K+
Sbjct: 564 LTKLTTSKL 565

BLAST of Carg12291 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 399.4 bits (1025), Expect = 3.4e-111
Identity = 228/541 (42.14%), Postives = 333/541 (61.55%), Query Frame = 0

Query: 11  IFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKISNG 70
           IFRS  P I  P   +L + ++ F   SS  ++  ++   +  S +Y +      ++++G
Sbjct: 34  IFRSKLPDIDIPN--HLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASG 93

Query: 71  LLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPKLM 130
           L +LGI K DV+MI   NS EF   F+G   IGA+ TT NP Y   EL KQ++ +  KL+
Sbjct: 94  LYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLI 153

Query: 131 ISVVELWDKVKDL--NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVGVKQ 190
           I+  +  DK+K+L  N+  +  D+  P        L F+ LI    D++      V +  
Sbjct: 154 ITHSQYVDKLKNLGENLTLITTDEPTP-----ENCLPFSTLI--TDDETNPFQETVDIGG 213

Query: 191 SDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN-------FLPM 250
            D AAL +SSGTTG  KGV+LTH++ ITS     + Q+ +GD    YL         LP+
Sbjct: 214 DDAAALPFSSGTTGLPKGVVLTHKSLITS-----VAQQVDGDNPNLYLKSNDVILCVLPL 273

Query: 251 FHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVVK 310
           FH++ L  +    L+ G T++ M KF +   L  +++++VT   +VPP+V+ALAK   V 
Sbjct: 274 FHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVN 333

Query: 311 KYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLE---NRTVGKR 370
            Y+LSS++ + SGAAPLGKEL D     +P A++ QGYGMTE   V+S+     +     
Sbjct: 334 SYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPT 393

Query: 371 NTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGW 430
            +GS GT+    E ++V ++T   L  NQ GEI +RG  +M  YLN+P+AT  TID+ GW
Sbjct: 394 KSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGW 453

Query: 431 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEA 490
           +HTGD+GY DE+ ++++VDR+KE+IK+KGFQV PAELE+LL++H  I DA V+P  D+ A
Sbjct: 454 LHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVA 513

Query: 491 GEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEK 540
           GEVP+A+VVRS  + +TEEDV ++VA QV  YKRL +V F+ S+PKS SGKILR++L  K
Sbjct: 514 GEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAK 560

BLAST of Carg12291 vs. TAIR10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2)

HSP 1 Score: 394.8 bits (1013), Expect = 8.5e-110
Identity = 224/542 (41.33%), Postives = 338/542 (62.36%), Query Frame = 0

Query: 9   DGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKIS 68
           D IFRS  P I  P   +L +  ++F N S +  +  +++  + +  +Y+ +  ++ K++
Sbjct: 20  DVIFRSRLPDIYIPN--HLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 79

Query: 69  NGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPK 128
            GL  LG++++DVVMI  PNS E  + F+    IGAI T+ NP +  +E++KQ + +  K
Sbjct: 80  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 139

Query: 129 LMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIVG--- 188
           L+++     DK+K+L    VL+     S+      L F++L       + SE P V    
Sbjct: 140 LIVTQSRYVDKIKNLQNDGVLI-VTTDSDAIPENCLRFSEL-------TQSEEPRVDSIP 199

Query: 189 --VKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN------ 248
             +   D  AL +SSGTTG  KGV+LTH+  +TS     + Q+ +G+    Y N      
Sbjct: 200 EKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTS-----VAQQVDGENPNLYFNRDDVIL 259

Query: 249 -FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 308
             LPMFH++ L  I    L+ G TI+ MPKF +   L  +++ KVT   VVPP+VLA+AK
Sbjct: 260 CVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAK 319

Query: 309 QSVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLE---NR 368
               +KY+LSS++ + SGAAPLGKEL D  +   P+A + QGYGMTE   V+++     +
Sbjct: 320 SPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAK 379

Query: 369 TVGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTI 428
                 +G+ GT+    E +I+  DT   LP N+ GEI +RG  +M GYLN+P AT  TI
Sbjct: 380 EPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTI 439

Query: 429 DKYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPY 488
           DK GW+HTGD+G+ D++ +L++VDR+KELIKYKGFQVAPAELE+LL+ HPEI D  V+  
Sbjct: 440 DKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAM 499

Query: 489 PDDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRR 536
            +++AGEVP+A+VVRS +S+++E+++ +FV+ QV  YKR+ +V F +S+PK+ SGKILR+
Sbjct: 500 KEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRK 546

BLAST of Carg12291 vs. Swiss-Prot
Match: sp|Q9M0X9|4CLL7_ARATH (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 2.8e-211
Identity = 362/546 (66.30%), Postives = 461/546 (84.43%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGI+RSLRP +V P+DPN S+ SFLFRNSSSYP++LAI D+++ DS+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  ++++G  +LGI KNDVV+IFAPNS +F +CF+ + AIG + TT NP+Y V+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEI---QSSKILCFNDLINMAGDKS 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL  +   EI    +SKIL F++++ ++  + 
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELS--EP 180

Query: 181 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 240
            SE+P V +KQSDTAALLYSSGTTG SKGV LTH NFI +SLM+T+DQ   G+ +G +L 
Sbjct: 181 VSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLC 240

Query: 241 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 300
           FLPMFHVFGL  IT +QLQRGN +VSM +F LE  L  +EK++VT LWVVPPV LAL+KQ
Sbjct: 241 FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQ 300

Query: 301 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 360
           S+VKK++LSSLK+IGSGAAPLGK+LM+EC  NIP+ +++QGYGMTETCG+VS+E+  +GK
Sbjct: 301 SIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGK 360

Query: 361 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 420
           RN+GSAG LA GVEAQIVSV+T K  PPNQ GEI VRGPNMM GYLNNPQATK+TIDK  
Sbjct: 361 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 420

Query: 421 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 480
           WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP+PD+E
Sbjct: 421 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 480

Query: 481 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 540
           AGEVPIA+VVRS NSS+TE+D+ KF+A QV+PYKRLRRV+FI+ VPKS +GKILRREL++
Sbjct: 481 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELVQ 540

Query: 541 KVRAKI 544
           +VR+K+
Sbjct: 541 QVRSKM 544

BLAST of Carg12291 vs. Swiss-Prot
Match: sp|Q0DV32|4CLL1_ORYSJ (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 565.8 bits (1457), Expect = 5.0e-160
Identity = 297/545 (54.50%), Postives = 394/545 (72.29%), Query Frame = 0

Query: 4   SGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSD-SVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R + + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  SAIKISNGL-LQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 123
           + +  +  L  + G+   D V++ APN V + +CF  + A+GA+ TT NP Y   E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQS----SKILCFNDLINMAGDKS 183
           V DA+ KL+I++  L  K+  L +P +LLD    +   S    + +  + +L+  AG K 
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLV--AGVKE 188

Query: 184 GSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN 243
            +++    +KQSDTAALLYSSGTTG SKGVILTHRNFI ++ M+T DQ    +    +L 
Sbjct: 189 -ADYRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLC 248

Query: 244 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 303
           FLPMFH+FGL  IT AQL RGN I++M +F++   + AV++++VT L+ VPPV++ALAK 
Sbjct: 249 FLPMFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKH 308

Query: 304 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 363
               KY+LSSLK IGSGAAPLGK++M+  A   P + ++QGYGMTETCG++SLE    G+
Sbjct: 309 GKAGKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQ 368

Query: 364 -RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKY 423
            R  GS GTL SGVEA+IV + TLK LPPNQ GEI VRGPN+M GY NN QAT+ TI K 
Sbjct: 369 AREFGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQ 428

Query: 424 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDD 483
           GW+HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP+PD 
Sbjct: 429 GWLHTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDA 488

Query: 484 EAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELI 542
           +AGEVPIAYVVRS +SS+TE DV KF+  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI
Sbjct: 489 KAGEVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLI 548

BLAST of Carg12291 vs. Swiss-Prot
Match: sp|Q84P21|4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 418.7 bits (1075), Expect = 9.9e-116
Identity = 218/540 (40.37%), Postives = 350/540 (64.81%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLK 62
           +SG+   +  F S R PI  P +P+L V +F+  +S ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQ 122
            +   +++ L ++GI K  VV++ +PNS+ F +  + ++++GAI+TT NP+   +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLMISVVELWDKVKDL--NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGS 182
           ++D+ P L  +  +L  K+      +P VL+D++    +   +      L+ M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFL 242
                 V Q DTA LLYSSGTTG SKGVI +HRN I + +   +++  + D    ++  +
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLI-AMVQTIVNRFGSDDGEQRFICTV 246

Query: 243 PMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK--Q 302
           PMFH++GL       L  G+TI+ + KF + + + A+ KY+ T L +VPP+++A+     
Sbjct: 247 PMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGAD 306

Query: 303 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGK 362
            +  KY+LSS+  +  G APL KE+ +  A   P+  +LQGYG+TE+ G+ +  +     
Sbjct: 307 QIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEES 366

Query: 363 RNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYG 422
           R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GY +N +AT  T+D  G
Sbjct: 367 RRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEG 426

Query: 423 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDE 482
           W+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP+PD E
Sbjct: 427 WLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKE 486

Query: 483 AGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIE 538
            G+ P+AYVVR T SS++E+ +++FVA QV+PYKR+R+V F++S+PK+ SGKILR++LI+
Sbjct: 487 VGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIK 538

BLAST of Carg12291 vs. Swiss-Prot
Match: sp|P14912|4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 4.2e-114
Identity = 229/545 (42.02%), Postives = 347/545 (63.67%), Query Frame = 0

Query: 9   DGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKIS 68
           D IFRS  P I  P+  +L + ++ F N S   ++  +++  + ++ +YSQ++  + K++
Sbjct: 10  DLIFRSKLPDIYIPK--HLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVA 69

Query: 69  NGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPK 128
           +GL +LGI++ D +M+  PNS E+   F+G    GAI T  NP +  +E+ KQ++ ++ K
Sbjct: 70  SGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAK 129

Query: 129 LMISVVELWDKVKDL----NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIV 188
           L+I+     DKVKD     NI  + +D      +  SK++          +   SE P V
Sbjct: 130 LIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLM----------EADESEMPEV 189

Query: 189 GVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN------- 248
            +   D  AL YSSGTTG  KGV+LTH+  +TS     + Q+ +GD    Y++       
Sbjct: 190 VINSDDVVALPYSSGTTGLPKGVMLTHKGLVTS-----VAQQVDGDNPNLYMHSEDVMIC 249

Query: 249 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 308
            LP+FH++ L  + C  L+ G TI+ M KF++   L  ++KYKVT    VPP+VLA+AK 
Sbjct: 250 ILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKS 309

Query: 309 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSL---ENRT 368
            VV KY+LSS++ + SGAAPLGKEL D      P+A + QGYGMTE   V+++     + 
Sbjct: 310 PVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 369

Query: 369 VGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTID 428
             +  +G+ GT+    E +IV  +T   LP NQ GEI +RG  +M GYLN+P++T+ TID
Sbjct: 370 PYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTID 429

Query: 429 KYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYP 488
           + GW+HTGD+G+ D++ +L++VDR+KE+IKYKGFQVAPAELEALL++HP I DA V+P  
Sbjct: 430 EEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMI 489

Query: 489 DDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRE 540
           D++AGEVP+A+VVR+   + TEE++ +FV+ QV  YKR+ RV F++++PKS SGKILR++
Sbjct: 490 DEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 537

BLAST of Carg12291 vs. Swiss-Prot
Match: sp|P14913|4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 5.4e-114
Identity = 230/545 (42.20%), Postives = 346/545 (63.49%), Query Frame = 0

Query: 9   DGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQLKASAIKIS 68
           D IFRS  P I  P+  +L + ++ F N S   ++  +++  + ++ +YSQ++  + K++
Sbjct: 10  DLIFRSKLPDIYIPK--HLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVA 69

Query: 69  NGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTKQVRDAKPK 128
           +GL +LGI++ D +M+  PNS E+   F+G    GAI T  NP +  +E+ KQ++ +  K
Sbjct: 70  SGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAK 129

Query: 129 LMISVVELWDKVKDL----NIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSEFPIV 188
           L+I+     DKVKD     NI  + +D      +  SK++          +   SE P V
Sbjct: 130 LIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLM----------EADESEMPEV 189

Query: 189 GVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLN------- 248
            +   D  AL YSSGTTG  KGV+LTH+  +TS     + Q+ +GD    Y++       
Sbjct: 190 VIDSDDVVALPYSSGTTGLPKGVMLTHKGLVTS-----VAQQVDGDNPNLYMHSEDVMIC 249

Query: 249 FLPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQ 308
            LP+FH++ L  + C  L+ G TI+ M KF++   L  ++KYKVT    VPP+VLA+AK 
Sbjct: 250 ILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKS 309

Query: 309 SVVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSL---ENRT 368
            VV KY+LSS++ + SGAAPLGKEL D      P+A + QGYGMTE   V+++     + 
Sbjct: 310 PVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKE 369

Query: 369 VGKRNTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTID 428
             +  +G+ GT+    E +IV  +T   LP NQ GEI +RG  +M GYLN+P++T+ TID
Sbjct: 370 PYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTID 429

Query: 429 KYGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYP 488
           + GW+HTGD+G+ D++ +L++VDR+KE+IKYKGFQVAPAELEALL++HP I DA V+P  
Sbjct: 430 EEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMI 489

Query: 489 DDEAGEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRE 540
           D++AGEVP+A+VVR+   + TEE++ +FV+ QV  YKR+ RV F++++PKS SGKILR++
Sbjct: 490 DEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKD 537

BLAST of Carg12291 vs. TrEMBL
Match: tr|A0A1S3CIB0|A0A1S3CIB0_CUCME (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 5.2e-279
Identity = 485/543 (89.32%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSG GRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVVMIFAPNSV+ SICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVKDLNIPTVLLDQ+IPS I SSKILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFI SSLMIT+DQ  +G+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDKYGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI++VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. TrEMBL
Match: tr|A0A0A0LXU2|A0A0A0LXU2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 2.0e-278
Identity = 483/543 (88.95%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Carg12291 vs. TrEMBL
Match: tr|A0A1D8KVT5|A0A1D8KVT5_MORAL (4-coumarate-CoA ligase 7 OS=Morus alba OX=3498 GN=4CL7 PE=2 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 6.0e-227
Identity = 397/543 (73.11%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+V P+DPNLS  SFLFRNSSSYPN+ A+++A+S + +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  K+S+G L LGI+KNDVV+IFAPNS+ F +CF+GI+A+GAI TT NP+Y VSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  + P  +   K++ FNDL+N++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAE-PKSV-PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTH NFI +SLM+ +DQ    + +  +L  LP
Sbjct: 181 FPSVNVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL  IT  QLQRGN IVSM KF+LEK L  VEKYK+T LW VPP+VLALAK SVV
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELM+ECA  IP  VV+QGYGMTETCG+VS+E+   GKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTL SGVEAQIV VDTLKPLPPNQ GEI VRGPNMM GYLNNPQATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP+PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSS+TEEDV KF+A+QV+P+KRLR+VTFINSVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

BLAST of Carg12291 vs. TrEMBL
Match: tr|A0A0M4KDV5|A0A0M4KDV5_MORAL (4-coumarate-CoA ligase 7-2 OS=Morus alba OX=3498 GN=4CL7-2 PE=2 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.0e-226
Identity = 397/543 (73.11%), Postives = 462/543 (85.08%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+V P+DPNLS  SFLFRNSSSYPN+ A+++A+S + +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++  K+S+G L LGI+KNDVV+IFAPNS+ F +CF+GI+A+GAI TT NP+Y VSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  + P  +   K++ FNDL+N++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAE-PKSV-PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTH NFI +SLM+ +DQ    + +  +L  LP
Sbjct: 181 FPSVKVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300
           MFHVFGL  IT  QLQRGN IVSM KF+LEK L  VEKYK+T LW VPP+VLALAK SVV
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360
           KKYNLSSLKHIGSGAAPLGKELM+ECA  IP  VV+QGYGMTETCG+VS+E+   GKRNT
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420
           GSAGTL SGVEAQIV VDTLKPLPPNQ GEI VRGPNMM GYLNNPQATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP+PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSS+TEEDV KF+A+QV+P+KRLR+VTFINSVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

BLAST of Carg12291 vs. TrEMBL
Match: tr|A0A2P6QGZ5|A0A2P6QGZ5_ROSCH (Putative AMP-dependent synthetase/ligase, AMP-binding enzyme domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0057631 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.1e-225
Identity = 395/545 (72.48%), Postives = 474/545 (86.97%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60
           MEKSGYGRDG+FRSLRPP+V PRDPNL++ SFLF+NSSSYP+R A++DAESS+++S+SQL
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPRDPNLTMVSFLFKNSSSYPHRPALIDAESSETLSFSQL 60

Query: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120
           K++ IK+++GL+ LGI+KNDVV+IFAPNS++F ICF+GI+AIGAI TT NP+Y VSEL+K
Sbjct: 61  KSTVIKVAHGLIHLGIKKNDVVLIFAPNSIQFPICFLGIIAIGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQ-IPSEIQ-SSKILCFNDLINMAGDKSG 180
           QV+D+ PKL+I+V ELW+KVK LN+P V+L  +   S+I+ SS+I+ F+DL+ ++G    
Sbjct: 121 QVKDSNPKLVITVPELWEKVKGLNLPAVILGPKGSSSQIESSSRIVGFHDLVQLSGPV-- 180

Query: 181 SEFPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNF 240
           S+FP V VKQ+DTAALLYSSGTTG SKGVILTH NFI SSLMIT DQ+  G+ +  YL  
Sbjct: 181 SDFPSVNVKQTDTAALLYSSGTTGLSKGVILTHGNFIASSLMITQDQEVAGEMHNVYLCV 240

Query: 241 LPMFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS 300
           LPMFHVFGL  IT AQL++GN ++SM +FNLEK L  VEK+KVT LW+VPP++LAL+K S
Sbjct: 241 LPMFHVFGLAVITYAQLRKGNAVISMSRFNLEKVLMTVEKFKVTHLWLVPPIILALSKDS 300

Query: 301 VVKKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKR 360
           VVKKY+LSSLKHIGSGAAPLGKELM+ECA  IP  VV QGYGMTETCG+VS+EN  VG R
Sbjct: 301 VVKKYSLSSLKHIGSGAAPLGKELMEECAKIIPQGVVSQGYGMTETCGIVSVENTLVGPR 360

Query: 361 NTGSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGW 420
           ++GSAGTL SGVE+QIVSVDTLKPLPP Q GEI VRGPNMM+GY NNPQAT+ T+DK GW
Sbjct: 361 HSGSAGTLVSGVESQIVSVDTLKPLPPKQLGEIWVRGPNMMMGYFNNPQATQLTLDKNGW 420

Query: 421 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEA 480
           VHTGDLGYFDE+GQL+VVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP+PD EA
Sbjct: 421 VHTGDLGYFDESGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEA 480

Query: 481 GEVPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEK 540
           GEVP+AYVVRS NSS+TEED+  F+A QV+ +KRLRRVTFIN+VPKS SGKILR+ELIEK
Sbjct: 481 GEVPVAYVVRSPNSSLTEEDIKSFIASQVASFKRLRRVTFINTVPKSASGKILRKELIEK 540

Query: 541 VRAKI 544
           VR+KI
Sbjct: 541 VRSKI 543

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022949355.11.2e-30699.824-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate-... [more]
XP_023525684.13.2e-30499.264-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-co... [more]
XP_022998874.11.0e-30298.534-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--C... [more]
XP_008463063.17.9e-27989.32PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo][more]
XP_004147290.13.0e-27888.95PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothet... [more]
Match NameE-valueIdentityDescription
AT4G05160.11.6e-21266.30AMP-dependent synthetase and ligase family protein[more]
AT1G20510.15.5e-11740.37OPC-8:0 CoA ligase1[more]
AT1G20480.14.1e-11242.08AMP-dependent synthetase and ligase family protein[more]
AT1G65060.13.4e-11142.144-coumarate:CoA ligase 3[more]
AT3G21240.18.5e-11041.334-coumarate:CoA ligase 2[more]
Match NameE-valueIdentityDescription
sp|Q9M0X9|4CLL7_ARATH2.8e-21166.304-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
sp|Q0DV32|4CLL1_ORYSJ5.0e-16054.504-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1... [more]
sp|Q84P21|4CLL5_ARATH9.9e-11640.374-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
sp|P14912|4CL1_PETCR4.2e-11442.024-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1[more]
sp|P14913|4CL2_PETCR5.4e-11442.204-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A1S3CIB0|A0A1S3CIB0_CUCME5.2e-27989.324-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1[more]
tr|A0A0A0LXU2|A0A0A0LXU2_CUCSA2.0e-27888.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1[more]
tr|A0A1D8KVT5|A0A1D8KVT5_MORAL6.0e-22773.114-coumarate-CoA ligase 7 OS=Morus alba OX=3498 GN=4CL7 PE=2 SV=1[more]
tr|A0A0M4KDV5|A0A0M4KDV5_MORAL1.0e-22673.114-coumarate-CoA ligase 7-2 OS=Morus alba OX=3498 GN=4CL7-2 PE=2 SV=1[more]
tr|A0A2P6QGZ5|A0A2P6QGZ5_ROSCH1.1e-22572.48Putative AMP-dependent synthetase/ligase, AMP-binding enzyme domain-containing p... [more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12291-RACarg12291-RAmRNA


Analysis Name: InterPro Annotations of silver-seed gourd
Date Performed: 2019-03-07
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 35..445
e-value: 1.8E-102
score: 343.0
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 454..529
e-value: 3.4E-15
score: 56.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 10..440
e-value: 1.9E-128
score: 430.9
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 441..542
e-value: 2.8E-29
score: 103.8
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 2..541
NoneNo IPR availablePANTHERPTHR24096:SF310SUBFAMILY NOT NAMEDcoord: 2..541
NoneNo IPR availableCDDcd059044CLcoord: 21..535
e-value: 0.0
score: 725.198
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 17..541
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Carg12291Silver-seed gourdcarcarB448
Carg12291Silver-seed gourdcarcarB450
Carg12291Cucurbita maxima (Rimu)carcmaB0611
Carg12291Cucurbita moschata (Rifu)carcmoB0594
Carg12291Cucurbita pepo (Zucchini)carcpeB0620