CsaV3_1G013520 (gene) Cucumber (Chinese Long) v3

NameCsaV3_1G013520
Typegene
OrganismCucumis sativus (Cucumber (Chinese Long) v3)
Description4-coumarate-CoA ligase
Locationchr1 : 8805865 .. 8809246 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACTTTCGACCATTATTTTTGATAAAATTCGAGTATAGAATATGGTGTCTAAGTGGAGGGTTAGTTGGCATTATTTGTAAAAGATGGGTCATTTTAGATATTAACGGACAAATTTCCAAATTTTTTATCACCTTCCTCTTTACCGGTCAAGGTTGAAGACGAAATCTCCAAATCTTCCCATTACCTACCACTCATGGCGACACTTTTTTCTTCCCTCGTGCTGCTGTAACAAGTCTTCACTCCTTATACAAGTCTCTCCTTATTTTGCTTCCATTTAACCGACTTTTGATGATGCCTCATTTTTCTCATATTCCTTGTCATCTTCACTTTCCTTTTCTCCCTCAAATTCCGCATCGGTCTGTGTGCTACTACTACTTATCCAAACCCTCTCTGCTAATACCTTTGTAATTTTCATTCAATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGTACCCTGTTGTAGGCTGTGTGTGTTCTATAACATGTTTTGTGTATTGTTTCTTGTTGGCTAAAATCAGCTTATCAGATTACAGAGGTAATTACTAAATCACCCTGATTGTTTTTAGAAATGTTCAAAATCTCTGACGGTGATAAAAACAATGAAAATGCTTTCAAGTTATTTTATTCGGTTAAAAATACCTCCCCTGTTCTAAGTCAAATACAATCAACTTAAATGTCGACTACTCCTGAATGGAAATTATTGTGGTTCTCACTTCATTTGACTTTGATTTACAACCTCGTAGAATTGAATTATTACATTCTAATGTAATGAACCGTTGAATTTGTTATCATTTTCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTATGATTGTGATTATCTTTTCATTGATTTTATGGTTTCATACGATACTTTGGAGAGAGTGTAAGCGTAGTCCCTGCATTTGTTTCAGTTAGAGTAACTATTATCATGAGCTAAGGTGATCTTTCCCATGTTAGGAGCCATTATTAGTACCTATGATTAAAGTCGGTTTTGAAATTCAATATTGAAATACCTCATTTACAGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTGAATATATGATATGTTTATCTTATCATATTTAGTTACGATCTGTACATTTTTAAAATTGAAGTAACATGAGCCGAGCTTCTTTGTTGCTATAACTAACATTGTTGTTGAGATGATACTGCATTCAACCGTTACCTTTCAAAACTTTCTTGCGACACTAATGACATATTTTTCAGTAATTTTGAAATAGTTAAAACCAGACTGACACTGAAACTTGGTTTTGAATAGTAAAAGATATACATACACTTCAAAGTAATTTTAACAATTTCAAAACCATCCACAAACATGTCCTAATATCCGGTAATTTCTCTATCGATGATAGCATGCTAGGTTAGTCGTTGTATGTGCAAATGCTGTTTGGAGTTCAAGCTTTTCTCTCTCTCTCATTTTTTGTTACTATATCTCCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCGTAAGCTTTCATTACTCATATTTAAACTTTTGAAGTTCATTTCTTAGTAACCATTTAACTGATACTATCAATTTTGAAACTTAATCGACAGCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTAAGCCACTTTAGAAGAAAGTTTACTGTTGTTCAACGTACATCATGAATTAAACTTACTTGTCCTTCTCTTGTAACAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGAGATCTTTGTTTCCTAGTTTCATTATGATAGTGGCCATAGAAGAGAAGCAATAAGTTGGTCTATAGACCTAATATTAAATTCATTTTGAGTGAATCTGAATAACTTGACTATAATAGTGAATAAATTATCTCTTTTTATTACAATATGTGTGTGAAAAGAGTGAAAAGACTCACTACTCAATAGTTATCTCGTTTGACATCCAT

mRNA sequence

ATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGA

Coding sequence (CDS)

ATGGAGAAATCTGGGTATGGTCGCGATGGAATTTTCCGGTCGTTACGGCCTCCGCTTGTCTTTCCTAAAGATCCCAATCTCTCCATGATTTCTTTCCTCTTCAGAAATTTATTGTCTTATCCAAATAGACTAGCCATTGTTGATGCGGAATCTTCAAATTCTGTCTCGTATTCCCAGCTTAAAGCCTTGGCAATTAGGGTATCCAATGGTTTGATCCAATTGGGTATTGAGAAAAATGACGTGGTGTTGATTTTTGCTCCAAATTCAGTTCAATTCACCATTTGTTTCATTGGGGTTATTGCCATTGGGGCTATTGTAACTACTTGTAATCCTGTATACACGGTTTCTGAGCTAACTAAGCAAGTTAGAGATGCTAAACCTAAACTCGTGATATCTGTTGCTGAATTGTGGGATAAAGTGAAGAATCTAAACATCCCAACTGTGTTACTAGACCAGCAAATTCCTTCAGCAATTCATTCTCCTAAAATATTGTGTTTCAATGATTTGGTGAACATGGCTGGTGATAAATCTGGGTCTGAGTTTCCAATTGTTGGGGTGAAGCAGAGTGATACAGCTGCACTTCTATATTCATCAGGAACAACAGGAGCTAGTAAGGGTGTGATTTTGACTCACGGAAACTTCATAGCATCTTCTTTGATGATAACAATGGATCAGACGTTTAATGGAGAGGAACATGGCGTATTTCTGAACTTCTTGCCAATGTTTCATGTGTTTGGATTGGCTTGTATAACATATGCTCAACTGCAGAAAGGAAACACCGTTGTCTCTATGCCAAAGTTTAATCTTGAGAAAGCCTTGTGGGCAGTGGAGAAGTACAAGGTGACAGATCTGTGGGTTGTGCCGCCTGTGGTGCTTGCTTTGGCAAAGCAGAGTCTGGTGAAGAAATACAACCTTTCATCCGTGAAGCGTGTCGGTTCAGGGGCTGCACCTCTTGGGAGGGAATTAATGGAGGAATGTGCTAATAACATTCCCAGTGCTGTGGTTATCCAGGGTTATGGTATGACTGAGACTTGTGGAGTTGTTGCTTTGGAGAATCCAGCAGTTGGCAAACGAAATTCTGGTTCAGCTGGAACACTTGCCTCTGGTGTTGAGGCTAAAATAGTCAGTGTAGATACTCTAAAGCCTCTACCTCCTAATCAATATGGCGAGATACATGTTCGAGGACCAAACATGATGCTCGGTTATTTCAACAATCCAGAAGCCACCAAGCAAACTATTGATAAACATGGATGGGTGCATACTGGAGACCTTGGATACTTCGATGAAAATGGTCAGCTTTATGTTGTCGACCGGATCAAGGAACTCATCAAGTACAAAGGTTTTCAGGTTGCACCAGCTGAGCTTGAAGCACTGCTGGTTTCTCACCCTGAGATACTAGATGCTGTTGTCATCCCCTGCCCTGATGAAGAAGCTGGAGAAGTTCCAATTGCTTATGTTGTTCGATCTGCCAATAGTTCTTTGACCGAAGAGGACATCCTTAAATTCATTGCTGATCAGGTTTCTCCTTATAAAAGGTTAAGAAGAGTGACATTCATCAGCAGTGTCCCCAAGTCTGTTTCTGGTAAAATTCTGAGGAGAGAACTCATTGAGAAAGTAAGAGCTAAAATCTGA

Protein sequence

MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVRAKI
BLAST of CsaV3_1G013520 vs. NCBI nr
Match: XP_004147290.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothetical protein Csa_1G108800 [Cucumis sativus])

HSP 1 Score: 1064.7 bits (2752), Expect = 1.1e-307
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. NCBI nr
Match: XP_008463063.1 (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo])

HSP 1 Score: 1032.3 bits (2668), Expect = 5.8e-298
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. NCBI nr
Match: XP_022949355.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 967.2 bits (2499), Expect = 2.3e-278
Identity = 482/543 (88.77%), Postives = 520/543 (95.76%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SFLFRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFLFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AK+
Sbjct: 541 AKL 543

BLAST of CsaV3_1G013520 vs. NCBI nr
Match: XP_022998874.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 963.4 bits (2489), Expect = 3.3e-277
Identity = 480/543 (88.40%), Postives = 519/543 (95.58%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP DPNLS+ SFLFRN  SYPNRLAIVDAESS+S SYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPTDPNLSVASFLFRNSSSYPNRLAIVDAESSDSASYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVYTVSEL+K
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK+LNIPTVLLD+QIP  I S KILCFNDL+NMAGDKSGSE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKDLNIPTVLLDKQIPLEIQSSKILCFNDLINMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGV+LTH NFI SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVVLTHRNFITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN AVGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRAVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. NCBI nr
Match: XP_023525684.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.1 bits (2483), Expect = 1.6e-276
Identity = 480/543 (88.40%), Postives = 517/543 (95.21%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPP+VFP+DPNLS+ SF+FRN  SYPNRLAIVDAESS+SVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPIVFPRDPNLSVASFIFRNSSSYPNRLAIVDAESSDSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KA AI++SNGL+QLGIEKNDVV+IFAPNSV+F+ICFIG++AIGAIVTTCNPVY VSELTK
Sbjct: 61  KASAIKISNGLLQLGIEKNDVVMIFAPNSVEFSICFIGIVAIGAIVTTCNPVYMVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKL+ISV ELWDKVK LNIPTVLLDQQIPS I S KILCFNDL+NMAGDKS SE
Sbjct: 121 QVRDAKPKLMISVVELWDKVKVLNIPTVLLDQQIPSEIQSSKILCFNDLINMAGDKSSSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH N I SSLMIT+DQ  NG+E+G +LNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNLITSSLMITLDQKTNGDENGTYLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGL CIT AQLQ+GNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS+V
Sbjct: 241 MFHVFGLVCITCAQLQRGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELM+ECANNIPSAVV+QGYGMTETCGVV+LEN  VGKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMDECANNIPSAVVLQGYGMTETCGVVSLENRTVGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEA+IVSVDTLKPLPPNQYGEI VRGPNMMLGY NNP+ATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAQIVSVDTLKPLPPNQYGEILVRGPNMMLGYLNNPQATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PD+EAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDDEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRS NSS+TEED+LKF+ADQVSPYKRLRRVTFI+SVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSTNSSMTEEDVLKFVADQVSPYKRLRRVTFINSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. TAIR10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 750.7 bits (1937), Expect = 6.1e-217
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of CsaV3_1G013520 vs. TAIR10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.2e-118
Identity = 226/541 (41.77%), Postives = 352/541 (65.06%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+   +  F S R P+  P +P+L + +F+  +  ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 122
                V++ L ++GI K  VVL+ +PNS+ F +  + V+++GAI+TT NP+ T +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLVISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGS 182
           ++D+ P L  + ++L  K+      +P VL+D++   ++   +      LV M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIAS-SLMITMDQTFNGEEHGVFLNF 242
                 V Q DTA LLYSSGTTG SKGVI +H N IA    ++    + +GE+   F+  
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR--FICT 246

Query: 243 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK-- 302
           +PMFH++GLA      L  G+T++ + KF + + + A+ KY+ T L +VPP+++A+    
Sbjct: 247 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 306

Query: 303 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 362
             +  KY+LSS+  V  G APL +E+ E  A   P+  ++QGYG+TE+ G+ A  +    
Sbjct: 307 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 366

Query: 363 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 422
            R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GYF+N EAT  T+D  
Sbjct: 367 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 426

Query: 423 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 482
           GW+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP PD+
Sbjct: 427 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 486

Query: 483 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 538
           E G+ P+AYVVR   SSL+E+ I++F+A QV+PYKR+R+V F+SS+PK+ SGKILR++LI
Sbjct: 487 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 538

BLAST of CsaV3_1G013520 vs. TAIR10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 416.4 bits (1069), Expect = 2.7e-116
Identity = 238/550 (43.27%), Postives = 339/550 (61.64%), Query Frame = 0

Query: 3   KSGYGRD-GIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNR--LAIVDAESSNSVSYSQ 62
           KSG+     IF S R P+  P +  L + SF    + S P+R     VDA +   +S+ +
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSF----IASQPHRGKTVFVDAVTGRRLSFPE 83

Query: 63  LKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELT 122
           L     RV+  L  LG+ K +VV+I +PNS+ F I  + V+++GAI+TT NP+ T  E++
Sbjct: 84  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 143

Query: 123 KQVRDAKPKLVISVAELWDKV---KNLNIPTVLLDQ-QIPSAIHSPKILCFNDLVNMAGD 182
           KQ+ D++P L  +  +L  K+    N N+P VL+D   +PS  +  ++     L  M   
Sbjct: 144 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 203

Query: 183 KSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVF 242
           +         V Q DTAALLYSSGTTG SKGV+L+H N IA  L+      F  E+  + 
Sbjct: 204 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--LVQAYRARFGLEQRTIC 263

Query: 243 LNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALA 302
              +PM H+FG        +  G T+V +PKF++ K L AVE ++ + L +VPP+V+A+ 
Sbjct: 264 T--IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 323

Query: 303 K--QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENP 362
                +  KY+LSS+  V +G APL RE+ E+   N P   ++QGYG+TE+  + A    
Sbjct: 324 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 383

Query: 363 AVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTI 422
               +  G++G LA  VE KIV  DT + L  NQ GE+ +R P +M GYF N EAT  TI
Sbjct: 384 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 443

Query: 423 DKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPC 482
           D  GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP 
Sbjct: 444 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 503

Query: 483 PDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRR 542
           PD +AG+ P+AY+VR   S+L+E +I+ F+A QVSPYK++R+VTF++S+PK+ SGKILRR
Sbjct: 504 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 563

Query: 543 ELIEKVRAKI 544
           EL +   +K+
Sbjct: 564 ELTKLTTSKL 565

BLAST of CsaV3_1G013520 vs. TAIR10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3)

HSP 1 Score: 401.7 bits (1031), Expect = 7.0e-112
Identity = 228/543 (41.99%), Postives = 337/543 (62.06%), Query Frame = 0

Query: 11  IFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVSNG 70
           IFRS  P +  P   +L + ++ F  L S  ++  ++   +  S +Y +   +  RV++G
Sbjct: 34  IFRSKLPDIDIPN--HLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASG 93

Query: 71  LIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPKLV 130
           L +LGI K DV++I   NS +F   F+G   IGA+ TT NP YT  EL KQ++ +  KL+
Sbjct: 94  LYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLI 153

Query: 131 ISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIVGVKQ 190
           I+ ++  DK+KNL  N+  +  D+  P        L F+ L+    D++      V +  
Sbjct: 154 ITHSQYVDKLKNLGENLTLITTDEPTPE-----NCLPFSTLI--TDDETNPFQETVDIGG 213

Query: 191 SDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLN-------FLPM 250
            D AAL +SSGTTG  KGV+LTH      SL+ ++ Q  +G+   ++L         LP+
Sbjct: 214 DDAAALPFSSGTTGLPKGVVLTH-----KSLITSVAQQVDGDNPNLYLKSNDVILCVLPL 273

Query: 251 FHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVK 310
           FH++ L  +    L+ G TV+ M KF +   L  +++++VT   +VPP+V+ALAK   V 
Sbjct: 274 FHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVN 333

Query: 311 KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVAL-----ENPAVG 370
            Y+LSSV+ V SGAAPLG+EL +     +P A++ QGYGMTE   V+++     + P   
Sbjct: 334 SYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPT 393

Query: 371 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 430
           K  SGS GT+    E K+V ++T   L  NQ GEI +RG  +M  Y N+PEAT  TID+ 
Sbjct: 394 K--SGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEE 453

Query: 431 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 490
           GW+HTGD+GY DE+ ++++VDR+KE+IK+KGFQV PAELE+LL++H  I DA V+P  DE
Sbjct: 454 GWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDE 513

Query: 491 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
            AGEVP+A+VVRS  + +TEED+ +++A QV  YKRL +V F++S+PKS SGKILR++L 
Sbjct: 514 VAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLK 560

BLAST of CsaV3_1G013520 vs. TAIR10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 400.2 bits (1027), Expect = 2.0e-111
Identity = 232/545 (42.57%), Postives = 342/545 (62.75%), Query Frame = 0

Query: 3   KSGYGR-DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+ + +  F S R PL  P  PNLS     F +   +  + A +DA +   +++S L 
Sbjct: 13  RSGFCKSNSTFYSKRQPLSLP--PNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLW 72

Query: 63  ALAIRVSNGLI-QLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 122
               RV++ L  ++GI + DVVLI +PNS+   +  + V+++GA+ TT N + T  E++K
Sbjct: 73  RAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISK 132

Query: 123 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 182
           Q+ D+ P LV +  +L  K+  + I  VL D ++   + S  I     L  M   +   +
Sbjct: 133 QIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTS-AIRVVGILSEMVKKEPSGQ 192

Query: 183 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 242
                V Q DTA +LYSSGTTG SKGVI +H N  A       D   N +   +F+  +P
Sbjct: 193 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISD---NLKRDDIFICTVP 252

Query: 243 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-L 302
           MFH +GL       +  G+TVV + +F L   + AVEK++ T L + PPV++A+   + L
Sbjct: 253 MFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADL 312

Query: 303 VK-KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR 362
           +K KY+LSS+K V  G APL +E+ E      P+  ++QGY +TE+ G  A  N A   R
Sbjct: 313 IKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESR 372

Query: 363 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 422
             G+AGTL S VEA+IV  +T + +  NQ GE+ ++GP++  GYF N EAT +TI+  GW
Sbjct: 373 RYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGW 432

Query: 423 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 482
           + TGDL Y DE+G L+VVDR+KELIKYKG+QV PAELEALL++HP+ILDA VIP PD+EA
Sbjct: 433 LKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEA 492

Query: 483 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 542
           G+ P+AYVVR   S+L+E+ ++ FI+ QV+PYK++R+V+FI+S+PK+ SGK LR++LI+ 
Sbjct: 493 GQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKL 550

Query: 543 VRAKI 544
             +K+
Sbjct: 553 ATSKL 550

BLAST of CsaV3_1G013520 vs. Swiss-Prot
Match: sp|Q9M0X9|4CLL7_ARATH (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.1e-215
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of CsaV3_1G013520 vs. Swiss-Prot
Match: sp|Q0DV32|4CLL1_ORYSJ (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 302/542 (55.72%), Postives = 393/542 (72.51%), Query Frame = 0

Query: 4   SGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAES-SNSVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R   + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  LAIRVSNGL-IQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 123
             +  +  L  + G+   D VL+ APN V + +CF  V A+GA+ TT NP YT  E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHS-PKILCFNDLVNMAGDKSGSE 183
           V DA+ KLVI+++ L  K+  L +P +LLD    +A  S P         N+      ++
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEAD 188

Query: 184 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 243
           +    +KQSDTAALLYSSGTTG SKGVILTH NFIA++ M+T DQ    E   VFL FLP
Sbjct: 189 YRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLP 248

Query: 244 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 303
           MFH+FGL+ ITYAQL +GN +++M +F++   + AV++++VT L+ VPPV++ALAK    
Sbjct: 249 MFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKA 308

Query: 304 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK-RN 363
            KY+LSS+K +GSGAAPLG+++ME  A   P + ++QGYGMTETCG+++LE P  G+ R 
Sbjct: 309 GKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQARE 368

Query: 364 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 423
            GS GTL SGVEAKIV + TLK LPPNQ GEI VRGPN+M GYFNN +AT+ TI K GW+
Sbjct: 369 FGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWL 428

Query: 424 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 483
           HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP PD +AG
Sbjct: 429 HTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAG 488

Query: 484 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 542
           EVPIAYVVRS +SSLTE D+ KFI  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI +V
Sbjct: 489 EVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQV 548

BLAST of CsaV3_1G013520 vs. Swiss-Prot
Match: sp|Q84P21|4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 4.0e-117
Identity = 226/541 (41.77%), Postives = 352/541 (65.06%), Query Frame = 0

Query: 3   KSGY-GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+   +  F S R P+  P +P+L + +F+  +  ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 122
                V++ L ++GI K  VVL+ +PNS+ F +  + V+++GAI+TT NP+ T +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLVISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGS 182
           ++D+ P L  + ++L  K+      +P VL+D++   ++   +      LV M   +   
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVR-----RLVEMMKKEPSG 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIAS-SLMITMDQTFNGEEHGVFLNF 242
                 V Q DTA LLYSSGTTG SKGVI +H N IA    ++    + +GE+   F+  
Sbjct: 187 NRVKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR--FICT 246

Query: 243 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK-- 302
           +PMFH++GLA      L  G+T++ + KF + + + A+ KY+ T L +VPP+++A+    
Sbjct: 247 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 306

Query: 303 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 362
             +  KY+LSS+  V  G APL +E+ E  A   P+  ++QGYG+TE+ G+ A  +    
Sbjct: 307 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 366

Query: 363 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 422
            R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GYF+N EAT  T+D  
Sbjct: 367 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 426

Query: 423 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 482
           GW+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP PD+
Sbjct: 427 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 486

Query: 483 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 538
           E G+ P+AYVVR   SSL+E+ I++F+A QV+PYKR+R+V F+SS+PK+ SGKILR++LI
Sbjct: 487 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 538

BLAST of CsaV3_1G013520 vs. Swiss-Prot
Match: sp|Q84P25|4CLL2_ARATH (4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=2)

HSP 1 Score: 416.4 bits (1069), Expect = 4.9e-115
Identity = 238/550 (43.27%), Postives = 339/550 (61.64%), Query Frame = 0

Query: 3   KSGYGRD-GIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNR--LAIVDAESSNSVSYSQ 62
           KSG+     IF S R P+  P +  L + SF    + S P+R     VDA +   +S+ +
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSF----IASQPHRGKTVFVDAVTGRRLSFPE 83

Query: 63  LKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELT 122
           L     RV+  L  LG+ K +VV+I +PNS+ F I  + V+++GAI+TT NP+ T  E++
Sbjct: 84  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 143

Query: 123 KQVRDAKPKLVISVAELWDKV---KNLNIPTVLLDQ-QIPSAIHSPKILCFNDLVNMAGD 182
           KQ+ D++P L  +  +L  K+    N N+P VL+D   +PS  +  ++     L  M   
Sbjct: 144 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 203

Query: 183 KSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVF 242
           +         V Q DTAALLYSSGTTG SKGV+L+H N IA  L+      F  E+  + 
Sbjct: 204 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--LVQAYRARFGLEQRTIC 263

Query: 243 LNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALA 302
              +PM H+FG        +  G T+V +PKF++ K L AVE ++ + L +VPP+V+A+ 
Sbjct: 264 T--IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 323

Query: 303 K--QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENP 362
                +  KY+LSS+  V +G APL RE+ E+   N P   ++QGYG+TE+  + A    
Sbjct: 324 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 383

Query: 363 AVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTI 422
               +  G++G LA  VE KIV  DT + L  NQ GE+ +R P +M GYF N EAT  TI
Sbjct: 384 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 443

Query: 423 DKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPC 482
           D  GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP 
Sbjct: 444 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 503

Query: 483 PDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRR 542
           PD +AG+ P+AY+VR   S+L+E +I+ F+A QVSPYK++R+VTF++S+PK+ SGKILRR
Sbjct: 504 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 563

Query: 543 ELIEKVRAKI 544
           EL +   +K+
Sbjct: 564 ELTKLTTSKL 565

BLAST of CsaV3_1G013520 vs. Swiss-Prot
Match: sp|P31685|4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 2.1e-113
Identity = 234/545 (42.94%), Postives = 340/545 (62.39%), Query Frame = 0

Query: 9   DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVS 68
           D IFRS  P +  PK  +L + S+ F NL  + +R  ++D  +    +Y++++  + +V+
Sbjct: 12  DLIFRSKLPDIYIPK--HLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 71

Query: 69  NGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPK 128
            GL +LGI++ D ++I  PN  +F   FIG   +GAI T  NP++T +E+ KQ + +  K
Sbjct: 72  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 129 LVISVAELWDKVKNL----NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIV 188
           +VI+ A    KVK+     ++  + +D      +H      F++L+         E P V
Sbjct: 132 IVITQACFAGKVKDYAIENDLKVICVDSAPEGCVH------FSELI----QSDEHEIPDV 191

Query: 189 GVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHG--VFLNFLPMF 248
            ++  D  AL YSSGTTG  KGV+LTH   + S       +  N   H   V +  LP+F
Sbjct: 192 KIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLF 251

Query: 249 HVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVKK 308
           H++ L  +    L+ G  ++ M KF++ + L  + K+KVT    VPP+VLA+AK  LV  
Sbjct: 252 HIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHN 311

Query: 309 YNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR---- 368
           Y+LSSV+ V SGAAPLG+EL +      P+A + QGYGMTE   V+A+   A  K     
Sbjct: 312 YDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAM-CLAFAKEPFDI 371

Query: 369 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 428
            SG+ GT+    E KIV  DT   LP NQ GEI +RG  +M GY N+PEAT +TI+K GW
Sbjct: 372 KSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGW 431

Query: 429 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 488
           +HTGD+G+ D++ +L++VDR+KELIKYKGFQVAPAELEALL++HP+I DA V+P  DE+A
Sbjct: 432 LHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQA 491

Query: 489 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 544
           GEVP+A+VVRS  S++TE+++  FI+ QV  YKR++RV F+ +VPKS SGKILR++L  +
Sbjct: 492 GEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRAR 543

BLAST of CsaV3_1G013520 vs. TrEMBL
Match: tr|A0A0A0LXU2|A0A0A0LXU2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 7.0e-308
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. TrEMBL
Match: tr|A0A1S3CIB0|A0A1S3CIB0_CUCME (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 3.8e-298
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of CsaV3_1G013520 vs. TrEMBL
Match: tr|A0A2P6QGZ5|A0A2P6QGZ5_ROSCH (Putative AMP-dependent synthetase/ligase, AMP-binding enzyme domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr5g0057631 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 2.5e-228
Identity = 403/545 (73.94%), Postives = 473/545 (86.79%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDG+FRSLRPPLV P+DPNL+M+SFLF+N  SYP+R A++DAESS ++S+SQL
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPRDPNLTMVSFLFKNSSSYPHRPALIDAESSETLSFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+  I+V++GLI LGI+KNDVVLIFAPNS+QF ICF+G+IAIGAI TT NP+YTVSEL+K
Sbjct: 61  KSTVIKVAHGLIHLGIKKNDVVLIFAPNSIQFPICFLGIIAIGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSA--IHSPKILCFNDLVNMAGDKSG 180
           QV+D+ PKLVI+V ELW+KVK LN+P V+L  +  S+    S +I+ F+DLV ++G    
Sbjct: 121 QVKDSNPKLVITVPELWEKVKGLNLPAVILGPKGSSSQIESSSRIVGFHDLVQLSGPV-- 180

Query: 181 SEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNF 240
           S+FP V VKQ+DTAALLYSSGTTG SKGVILTHGNFIASSLMIT DQ   GE H V+L  
Sbjct: 181 SDFPSVNVKQTDTAALLYSSGTTGLSKGVILTHGNFIASSLMITQDQEVAGEMHNVYLCV 240

Query: 241 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS 300
           LPMFHVFGLA ITYAQL+KGN V+SM +FNLEK L  VEK+KVT LW+VPP++LAL+K S
Sbjct: 241 LPMFHVFGLAVITYAQLRKGNAVISMSRFNLEKVLMTVEKFKVTHLWLVPPIILALSKDS 300

Query: 301 LVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR 360
           +VKKY+LSS+K +GSGAAPLG+ELMEECA  IP  VV QGYGMTETCG+V++EN  VG R
Sbjct: 301 VVKKYSLSSLKHIGSGAAPLGKELMEECAKIIPQGVVSQGYGMTETCGIVSVENTLVGPR 360

Query: 361 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 420
           +SGSAGTL SGVE++IVSVDTLKPLPP Q GEI VRGPNMM+GYFNNP+AT+ T+DK+GW
Sbjct: 361 HSGSAGTLVSGVESQIVSVDTLKPLPPKQLGEIWVRGPNMMMGYFNNPQATQLTLDKNGW 420

Query: 421 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 480
           VHTGDLGYFDE+GQL+VVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP PD EA
Sbjct: 421 VHTGDLGYFDESGQLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEA 480

Query: 481 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 540
           GEVP+AYVVRS NSSLTEEDI  FIA QV+ +KRLRRVTFI++VPKS SGKILR+ELIEK
Sbjct: 481 GEVPVAYVVRSPNSSLTEEDIKSFIASQVASFKRLRRVTFINTVPKSASGKILRKELIEK 540

Query: 541 VRAKI 544
           VR+KI
Sbjct: 541 VRSKI 543

BLAST of CsaV3_1G013520 vs. TrEMBL
Match: tr|A0A1D8KVT5|A0A1D8KVT5_MORAL (4-coumarate-CoA ligase 7 OS=Morus alba OX=3498 GN=4CL7 PE=2 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 7.1e-228
Identity = 398/543 (73.30%), Postives = 463/543 (85.27%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV PKDPNLS +SFLFRN  SYPN+ A+++A+S   +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   +VS+G + LGI+KNDVVLIFAPNS+ F +CF+G+IA+GAI TT NP+YTVSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  + P ++   K++ FNDLVN++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAE-PKSV-PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTHGNFIA+SLM+ MDQ    E H VFL  LP
Sbjct: 181 FPSVNVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLA ITY QLQ+GN +VSM KF+LEK L  VEKYK+T LW VPP+VLALAK S+V
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELMEECA  IP  VVIQGYGMTETCG+V++E+   GKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTL SGVEA+IV VDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSSLTEED+ KFIA+QV+P+KRLR+VTFI+SVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

BLAST of CsaV3_1G013520 vs. TrEMBL
Match: tr|A0A0M4KDV5|A0A0M4KDV5_MORAL (4-coumarate-CoA ligase 7-2 OS=Morus alba OX=3498 GN=4CL7-2 PE=2 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 1.2e-227
Identity = 398/543 (73.30%), Postives = 463/543 (85.27%), Query Frame = 0

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV PKDPNLS +SFLFRN  SYPN+ A+++A+S   +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   +VS+G + LGI+KNDVVLIFAPNS+ F +CF+G+IA+GAI TT NP+YTVSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  + P ++   K++ FNDLVN++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAE-PKSV-PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTHGNFIA+SLM+ MDQ    E H VFL  LP
Sbjct: 181 FPSVKVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLA ITY QLQ+GN +VSM KF+LEK L  VEKYK+T LW VPP+VLALAK S+V
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELMEECA  IP  VVIQGYGMTETCG+V++E+   GKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTL SGVEA+IV VDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSSLTEED+ KFIA+QV+P+KRLR+VTFI+SVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004147290.11.1e-307100.00PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis sativus] >KGN64806.1 hypothet... [more]
XP_008463063.15.8e-29897.05PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo][more]
XP_022949355.12.3e-27888.774-coumarate--CoA ligase-like 7 [Cucurbita moschata] >XP_022949356.1 4-coumarate-... [more]
XP_022998874.13.3e-27788.404-coumarate--CoA ligase-like 7 [Cucurbita maxima] >XP_022998875.1 4-coumarate--C... [more]
XP_023525684.11.6e-27688.404-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo] >XP_023525685.1 4-co... [more]
Match NameE-valueIdentityDescription
AT4G05160.16.1e-21768.37AMP-dependent synthetase and ligase family protein[more]
AT1G20510.12.2e-11841.77OPC-8:0 CoA ligase1[more]
AT1G20480.12.7e-11643.27AMP-dependent synthetase and ligase family protein[more]
AT1G65060.17.0e-11241.994-coumarate:CoA ligase 3[more]
AT1G20500.12.0e-11142.57AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
sp|Q9M0X9|4CLL7_ARATH1.1e-21568.374-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=... [more]
sp|Q0DV32|4CLL1_ORYSJ1.5e-16455.724-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1... [more]
sp|Q84P21|4CLL5_ARATH4.0e-11741.774-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=... [more]
sp|Q84P25|4CLL2_ARATH4.9e-11543.274-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana OX=3702 GN=4CLL2 PE=2 SV=... [more]
sp|P31685|4CL2_SOLTU2.1e-11342.944-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
tr|A0A0A0LXU2|A0A0A0LXU2_CUCSA7.0e-308100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G108800 PE=4 SV=1[more]
tr|A0A1S3CIB0|A0A1S3CIB0_CUCME3.8e-29897.054-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103501298 PE=4 SV=1[more]
tr|A0A2P6QGZ5|A0A2P6QGZ5_ROSCH2.5e-22873.94Putative AMP-dependent synthetase/ligase, AMP-binding enzyme domain-containing p... [more]
tr|A0A1D8KVT5|A0A1D8KVT5_MORAL7.1e-22873.304-coumarate-CoA ligase 7 OS=Morus alba OX=3498 GN=4CL7 PE=2 SV=1[more]
tr|A0A0M4KDV5|A0A0M4KDV5_MORAL1.2e-22773.304-coumarate-CoA ligase 7-2 OS=Morus alba OX=3498 GN=4CL7-2 PE=2 SV=1[more]
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: INTERPRO
TermDefinition
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
IPR000873AMP-dep_Synth/Lig
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0044710 single-organism metabolic process
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0019643 reductive tricarboxylic acid cycle
cellular_component GO:0005777 peroxisome
cellular_component GO:0005836 fatty-acyl-CoA synthase complex
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003987 acetate-CoA ligase activity
molecular_function GO:0004321 fatty-acyl-CoA synthase activity
molecular_function GO:0047077 Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing) activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_1G013520.1CsaV3_1G013520.1mRNA


Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 38..445
e-value: 7.0E-106
score: 354.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.12780coord: 10..440
e-value: 1.9E-130
score: 437.5
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 441..542
e-value: 1.7E-29
score: 104.4
NoneNo IPR availablePANTHERPTHR24096:SF310SUBFAMILY NOT NAMEDcoord: 2..541
NoneNo IPR availablePANTHERPTHR24096FAMILY NOT NAMEDcoord: 2..541
NoneNo IPR availableCDDcd059044CLcoord: 21..535
e-value: 0.0
score: 729.436
NoneNo IPR availableSUPERFAMILYSSF56801Acetyl-CoA synthetase-likecoord: 17..541
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 454..529
e-value: 5.5E-15
score: 56.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205