Cucsa.292580 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.292580 vs. Swiss-Prot
Match: 4CLL7_ARATH (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 9.8e-217
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of Cucsa.292580 vs. Swiss-Prot
Match: 4CLL1_ORYSJ (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-165
Identity = 302/542 (55.72%), Postives = 393/542 (72.51%), Query Frame = 1

Query: 4   SGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSN-SVSYSQLKA 63
           +GYG DG++RSLRPP     DP LS+   L R   + P+ +A+ DA +   ++++++L++
Sbjct: 9   AGYGADGVYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRS 68

Query: 64  LAIRVSNGLI-QLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 123
             +  +  L  + G+   D VL+ APN V + +CF  V A+GA+ TT NP YT  E+ KQ
Sbjct: 69  AVLSTAVALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQ 128

Query: 124 VRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHS-PKILCFNDLVNMAGDKSGSE 183
           V DA+ KLVI+++ L  K+  L +P +LLD    +A  S P         N+      ++
Sbjct: 129 VSDARAKLVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEAD 188

Query: 184 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 243
           +    +KQSDTAALLYSSGTTG SKGVILTH NFIA++ M+T DQ    E   VFL FLP
Sbjct: 189 YRRPPIKQSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLP 248

Query: 244 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 303
           MFH+FGL+ ITYAQL +GN +++M +F++   + AV++++VT L+ VPPV++ALAK    
Sbjct: 249 MFHIFGLSVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKA 308

Query: 304 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGK-RN 363
            KY+LSS+K +GSGAAPLG+++ME  A   P + ++QGYGMTETCG+++LE P  G+ R 
Sbjct: 309 GKYDLSSLKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKGQARE 368

Query: 364 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 423
            GS GTL SGVEAKIV + TLK LPPNQ GEI VRGPN+M GYFNN +AT+ TI K GW+
Sbjct: 369 FGSTGTLVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWL 428

Query: 424 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 483
           HTGDLGYFD  GQL+VVDR+KELIKYKGFQ+APAELE LL+SHPEILDAVVIP PD +AG
Sbjct: 429 HTGDLGYFDGGGQLFVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDAKAG 488

Query: 484 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 542
           EVPIAYVVRS +SSLTE D+ KFI  QV+ YKRL+RVTF+ SVPKS SGKILRR+LI +V
Sbjct: 489 EVPIAYVVRSPDSSLTEVDVQKFIEKQVAYYKRLKRVTFVGSVPKSASGKILRRQLIAQV 548

BLAST of Cucsa.292580 vs. Swiss-Prot
Match: 4CLL5_ARATH (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 3.6e-118
Identity = 227/541 (41.96%), Postives = 354/541 (65.43%), Query Frame = 1

Query: 3   KSGY-GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+   +  F S R P+  P +P+L + +F+     ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 122
                V++ L ++GI K  VVL+ +PNS+ F +  + V+++GAI+TT NP+ T +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLVISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGS 182
           ++D+ P L  + ++L  K+      +P VL+D++   ++   + L    +  M  + SG+
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRL----VEMMKKEPSGN 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIAS-SLMITMDQTFNGEEHGVFLNF 242
                 V Q DTA LLYSSGTTG SKGVI +H N IA    ++    + +GE+   F+  
Sbjct: 187 RVK-ERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR--FICT 246

Query: 243 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK-- 302
           +PMFH++GLA      L  G+T++ + KF + + + A+ KY+ T L +VPP+++A+    
Sbjct: 247 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 306

Query: 303 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 362
             +  KY+LSS+  V  G APL +E+ E  A   P+  ++QGYG+TE+ G+ A  +    
Sbjct: 307 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 366

Query: 363 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 422
            R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GYF+N EAT  T+D  
Sbjct: 367 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 426

Query: 423 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 482
           GW+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP PD+
Sbjct: 427 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 486

Query: 483 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 538
           E G+ P+AYVVR   SSL+E+ I++F+A QV+PYKR+R+V F+SS+PK+ SGKILR++LI
Sbjct: 487 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 538

BLAST of Cucsa.292580 vs. Swiss-Prot
Match: 4CLL2_ARATH (4-coumarate--CoA ligase-like 2 OS=Arabidopsis thaliana GN=4CLL2 PE=2 SV=2)

HSP 1 Score: 419.9 bits (1078), Expect = 4.4e-116
Identity = 238/550 (43.27%), Postives = 339/550 (61.64%), Query Frame = 1

Query: 3   KSGYGRD-GIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNR--LAIVDAESSNSVSYSQ 62
           KSG+     IF S R P+  P +  L + SF+     S P+R     VDA +   +S+ +
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSFI----ASQPHRGKTVFVDAVTGRRLSFPE 83

Query: 63  LKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELT 122
           L     RV+  L  LG+ K +VV+I +PNS+ F I  + V+++GAI+TT NP+ T  E++
Sbjct: 84  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 143

Query: 123 KQVRDAKPKLVISVAELWDKV---KNLNIPTVLLDQ-QIPSAIHSPKILCFNDLVNMAGD 182
           KQ+ D++P L  +  +L  K+    N N+P VL+D   +PS  +  ++     L  M   
Sbjct: 144 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 203

Query: 183 KSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVF 242
           +         V Q DTAALLYSSGTTG SKGV+L+H N IA  L+      F  E+  + 
Sbjct: 204 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--LVQAYRARFGLEQRTIC 263

Query: 243 LNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALA 302
              +PM H+FG        +  G T+V +PKF++ K L AVE ++ + L +VPP+V+A+ 
Sbjct: 264 T--IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 323

Query: 303 K--QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENP 362
                +  KY+LSS+  V +G APL RE+ E+   N P   ++QGYG+TE+  + A    
Sbjct: 324 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 383

Query: 363 AVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTI 422
               +  G++G LA  VE KIV  DT + L  NQ GE+ +R P +M GYF N EAT  TI
Sbjct: 384 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 443

Query: 423 DKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPC 482
           D  GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP 
Sbjct: 444 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 503

Query: 483 PDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRR 542
           PD +AG+ P+AY+VR   S+L+E +I+ F+A QVSPYK++R+VTF++S+PK+ SGKILRR
Sbjct: 504 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 563

Query: 543 ELIEKVRAKI 544
           EL +   +K+
Sbjct: 564 ELTKLTTSKL 565

BLAST of Cucsa.292580 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 415.6 bits (1067), Expect = 8.3e-115
Identity = 234/546 (42.86%), Postives = 341/546 (62.45%), Query Frame = 1

Query: 9   DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVS 68
           D IFRS  P +  PK  +L + S+ F NL  + +R  ++D  +    +Y++++  + +V+
Sbjct: 12  DLIFRSKLPDIYIPK--HLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVA 71

Query: 69  NGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPK 128
            GL +LGI++ D ++I  PN  +F   FIG   +GAI T  NP++T +E+ KQ + +  K
Sbjct: 72  VGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAK 131

Query: 129 LVISVAELWDKVKNL----NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIV 188
           +VI+ A    KVK+     ++  + +D      +H      F++L+         E P V
Sbjct: 132 IVITQACFAGKVKDYAIENDLKVICVDSAPEGCVH------FSELIQ----SDEHEIPDV 191

Query: 189 GVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHG--VFLNFLPMF 248
            ++  D  AL YSSGTTG  KGV+LTH   + S       +  N   H   V +  LP+F
Sbjct: 192 KIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLF 251

Query: 249 HVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVKK 308
           H++ L  +    L+ G  ++ M KF++ + L  + K+KVT    VPP+VLA+AK  LV  
Sbjct: 252 HIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHN 311

Query: 309 YNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVAL-----ENPAVGK 368
           Y+LSSV+ V SGAAPLG+EL +      P+A + QGYGMTE   V+A+     + P   K
Sbjct: 312 YDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIK 371

Query: 369 RNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHG 428
             SG+ GT+    E KIV  DT   LP NQ GEI +RG  +M GY N+PEAT +TI+K G
Sbjct: 372 --SGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEG 431

Query: 429 WVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEE 488
           W+HTGD+G+ D++ +L++VDR+KELIKYKGFQVAPAELEALL++HP+I DA V+P  DE+
Sbjct: 432 WLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQ 491

Query: 489 AGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIE 544
           AGEVP+A+VVRS  S++TE+++  FI+ QV  YKR++RV F+ +VPKS SGKILR++L  
Sbjct: 492 AGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRA 543

BLAST of Cucsa.292580 vs. TrEMBL
Match: A0A0A0LXU2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G108800 PE=4 SV=1)

HSP 1 Score: 1068.1 bits (2761), Expect = 3.4e-309
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsa.292580 vs. TrEMBL
Match: A0A0M4KDV5_MORAL (4-coumarate-CoA ligase 7-2 OS=Morus alba GN=4CL7-2 PE=2 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 6.0e-229
Identity = 398/543 (73.30%), Postives = 461/543 (84.90%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV PKDPNLS +SFLFRN  SYPN+ A+++A+S   +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   +VS+G + LGI+KNDVVLIFAPNS+ F +CF+G+IA+GAI TT NP+YTVSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  +  S     K++ FNDLVN++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAEPKSV--PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTHGNFIA+SLM+ MDQ    E H VFL  LP
Sbjct: 181 FPSVKVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLA ITY QLQ+GN +VSM KF+LEK L  VEKYK+T LW VPP+VLALAK S+V
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELMEECA  IP  VVIQGYGMTETCG+V++E+   GKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTL SGVEA+IV VDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSSLTEED+ KFIA+QV+P+KRLR+VTFI+SVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

BLAST of Cucsa.292580 vs. TrEMBL
Match: W9SXG6_9ROSA (4-coumarate--CoA ligase-like 7 OS=Morus notabilis GN=L484_020607 PE=4 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 8.6e-228
Identity = 397/538 (73.79%), Postives = 458/538 (85.13%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV PKDPNLS +SFLFRN  SYPN+ A++DA+S   +S+SQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALIDADSGEILSFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   +VS+G + LGI+KNDVVLIFAPNS+ F +CF+G+IAIGAI TT NP+YTVSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAIGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  +  S     K+L FN LVN++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAEHKSV--PKKVLTFNGLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTHGNFIA+SLM+ MDQ    E H VFL  LP
Sbjct: 181 FPSVNVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQEIAKEMHNVFLCVLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLA ITY+QLQ+GN VVSM KF+LEK L  VE+YK+T  WVVPP+VLALAK S+V
Sbjct: 241 MFHVFGLAVITYSQLQRGNAVVSMAKFDLEKILMTVERYKITHFWVVPPIVLALAKNSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELMEECA  IP  VVIQGYGMTETCG+V++E+   GKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTL SGVEA+IV VDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK T+DK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTMDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEILDAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 539
           VP+AYVVRS NSSLTEED+ KFIA+QV+P+KRLR+VTFI+SVPKS SGKILRRELIEK
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEK 534

BLAST of Cucsa.292580 vs. TrEMBL
Match: M5W8J3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003893mg PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.2e-226
Identity = 399/544 (73.35%), Postives = 465/544 (85.48%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV P+D NLSM+SFLFRN  SY N+ A++DAESS ++S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPRDLNLSMVSFLFRNSSSYSNKPALIDAESSETLSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+L I+VS+GL+ LGI+KNDVVLIFAPNS+QF ICF+G+IA GAI TT NP+YTVSEL+K
Sbjct: 61  KSLVIKVSHGLLHLGIKKNDVVLIFAPNSIQFPICFLGIIASGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHS-PKILCFNDLVNMAGDKSGS 180
           QV+D+ PKLVI+V EL++KVK+ N+P V+L  Q  S I S  KIL F+DLV  AG  S S
Sbjct: 121 QVKDSNPKLVITVPELFEKVKDFNLPAVILGSQGASHIASRSKILTFHDLVESAG--SAS 180

Query: 181 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFL 240
           +FP+  +KQ+DTAALLYSSGTTG SKGV+LTH NFIASSLM TM+Q   GE H VFL  L
Sbjct: 181 DFPLANIKQTDTAALLYSSGTTGMSKGVVLTHKNFIASSLMCTMEQEMTGEMHHVFLCVL 240

Query: 241 PMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSL 300
           PMFHVFGLA ITY+QLQKGN VVSM +F+L+K L AVEKYKVT LWVVPP+VLALAK S 
Sbjct: 241 PMFHVFGLAIITYSQLQKGNAVVSMSRFDLQKILMAVEKYKVTHLWVVPPIVLALAKNSA 300

Query: 301 VKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRN 360
           VKK+NLSS++ +GSGAAPLG+ELMEECA  IP  ++ QGYGMTETCG+ ++EN  VG R+
Sbjct: 301 VKKFNLSSLRHIGSGAAPLGKELMEECAKVIPQGLIAQGYGMTETCGIASVENTLVGPRH 360

Query: 361 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 420
           +GS G L SGVE +IVSVDTLKP PPN+ GEI VRGPNMM GYFNNP+AT +TIDK+GWV
Sbjct: 361 TGSTGMLVSGVECQIVSVDTLKPQPPNKLGEIWVRGPNMMSGYFNNPQATNETIDKNGWV 420

Query: 421 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 480
           HTGDLGYFDE+GQLYVVDRIKELIKYKGFQVAPAELE LL+SHPEILDAVVIP PD EAG
Sbjct: 421 HTGDLGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLISHPEILDAVVIPFPDAEAG 480

Query: 481 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 540
           EVP+AY VR+ NSSLTEED+  FIA+QV+P+KRLRRVTFI+SVPKS SGKILRRELI+KV
Sbjct: 481 EVPVAYCVRTPNSSLTEEDVKNFIANQVAPFKRLRRVTFINSVPKSASGKILRRELIDKV 540

Query: 541 RAKI 544
           R+KI
Sbjct: 541 RSKI 542

BLAST of Cucsa.292580 vs. TrEMBL
Match: A0A089WWI7_BETPN (4-coumarate:CoA ligase-like protein OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.2e-226
Identity = 395/547 (72.21%), Postives = 464/547 (84.83%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RS+RPPLV PKDPNLSM++FLFRN  SYP++ A++DA+SS ++S+SQ 
Sbjct: 1   MEKSGYGRDGIYRSVRPPLVLPKDPNLSMVTFLFRNSSSYPHKPALIDADSSQTLSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+    +S G + LGI KNDVVLIFAPNS+QF +CF+G++AIGAI TT NP+YTV EL +
Sbjct: 61  KSTVATISRGFLGLGITKNDVVLIFAPNSIQFPLCFLGIVAIGAIATTANPLYTVHELAR 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNM----AGDK 180
           QV+D+ PKL+I+V ELWDKVKN N+P V L     S+  S  ++ F+DLVN+    +G +
Sbjct: 121 QVKDSNPKLIITVPELWDKVKNFNLPAVFLGSNHSSS--SSNVIHFDDLVNLGGTWSGSR 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
           SGS+FP   VKQ+DTAALLYSSGTTG SKGVILTHGNFIA+SLM++MDQ  +GE H VFL
Sbjct: 181 SGSDFPDSNVKQNDTAALLYSSGTTGMSKGVILTHGNFIAASLMVSMDQELSGEAHNVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
             LPMFHVFGLA I Y+QLQ GN VVSM +F LEK L AVE+Y+V+ LWVVPP++LALAK
Sbjct: 241 CVLPMFHVFGLAVILYSQLQIGNAVVSMGRFELEKILRAVERYRVSHLWVVPPIILALAK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           Q +VKKY+LSS+K VGSGAAPLG++LMEECA NIP AVV QGYGMTETCGVV++ENP VG
Sbjct: 301 QDVVKKYDLSSLKHVGSGAAPLGKDLMEECARNIPQAVVAQGYGMTETCGVVSVENPRVG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
            R++GSAG L SGVE++IVSVDTLKPLPPNQ GEI VRGPNMM GY+NNP+ATK TIDK 
Sbjct: 361 VRHTGSAGLLVSGVESQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYYNNPQATKLTIDKQ 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
           GWVHTGDLGYFD +G LYVVDRIKELIKYKGFQVAPAELE LL+SHPEILDAVVIP PD 
Sbjct: 421 GWVHTGDLGYFDGDGHLYVVDRIKELIKYKGFQVAPAELEGLLISHPEILDAVVIPFPDA 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIAYVVRS NSSLTE D +KFIA+QV+P+KRLRRVTFI+ VPKS SGKILRRELI
Sbjct: 481 EAGEVPIAYVVRSPNSSLTEVDAMKFIANQVAPFKRLRRVTFINKVPKSASGKILRRELI 540

Query: 541 EKVRAKI 544
           EKV +K+
Sbjct: 541 EKVGSKM 545

BLAST of Cucsa.292580 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 754.2 bits (1946), Expect = 5.5e-218
Identity = 374/547 (68.37%), Postives = 463/547 (84.64%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGI+RSLRP LV PKDPN S++SFLFRN  SYP++LAI D+++ +S+++SQL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   R+++G  +LGI KNDVVLIFAPNS QF +CF+ V AIG + TT NP+YTV+E++K
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQ----QIPSAIHSPKILCFNDLVNMAGDK 180
           Q++D+ PK++ISV +L+DK+K  ++P VLL      +IP   +S KIL F++++ ++  +
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNS-KILSFDNVMELS--E 180

Query: 181 SGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFL 240
             SE+P V +KQSDTAALLYSSGTTG SKGV LTHGNFIA+SLM+TMDQ   GE HGVFL
Sbjct: 181 PVSEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFL 240

Query: 241 NFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK 300
            FLPMFHVFGLA ITY+QLQ+GN +VSM +F LE  L  +EK++VT LWVVPPV LAL+K
Sbjct: 241 CFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSK 300

Query: 301 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 360
           QS+VKK++LSS+K +GSGAAPLG++LMEEC  NIP+ +++QGYGMTETCG+V++E+P +G
Sbjct: 301 QSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLG 360

Query: 361 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 420
           KRNSGSAG LA GVEA+IVSV+T K  PPNQ GEI VRGPNMM GY NNP+ATK+TIDK 
Sbjct: 361 KRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKK 420

Query: 421 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 480
            WVHTGDLGYF+E+G LYVVDRIKELIKYKGFQVAPAELE LLVSHP+ILDAVVIP PDE
Sbjct: 421 SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDE 480

Query: 481 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
           EAGEVPIA+VVRS NSS+TE+DI KFIA QV+PYKRLRRV+FIS VPKS +GKILRREL+
Sbjct: 481 EAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRELV 540

Query: 541 EKVRAKI 544
           ++VR+K+
Sbjct: 541 QQVRSKM 544

BLAST of Cucsa.292580 vs. TAIR10
Match: AT1G20510.1 (AT1G20510.1 OPC-8:0 CoA ligase1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.0e-119
Identity = 227/541 (41.96%), Postives = 354/541 (65.43%), Query Frame = 1

Query: 3   KSGY-GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+   +  F S R P+  P +P+L + +F+     ++  R+A +DA +  ++++++L 
Sbjct: 7   RSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQ--AHRGRIAFIDASTGQNLTFTELW 66

Query: 63  ALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQ 122
                V++ L ++GI K  VVL+ +PNS+ F +  + V+++GAI+TT NP+ T +E+ KQ
Sbjct: 67  RAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQ 126

Query: 123 VRDAKPKLVISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGS 182
           ++D+ P L  + ++L  K+      +P VL+D++   ++   + L    +  M  + SG+
Sbjct: 127 IKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRL----VEMMKKEPSGN 186

Query: 183 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIAS-SLMITMDQTFNGEEHGVFLNF 242
                 V Q DTA LLYSSGTTG SKGVI +H N IA    ++    + +GE+   F+  
Sbjct: 187 RVK-ERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQR--FICT 246

Query: 243 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAK-- 302
           +PMFH++GLA      L  G+T++ + KF + + + A+ KY+ T L +VPP+++A+    
Sbjct: 247 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 306

Query: 303 QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVG 362
             +  KY+LSS+  V  G APL +E+ E  A   P+  ++QGYG+TE+ G+ A  +    
Sbjct: 307 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEE 366

Query: 363 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 422
            R  G+AG L++ +E +IV   T + L P Q GE+ ++GP++M GYF+N EAT  T+D  
Sbjct: 367 SRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSE 426

Query: 423 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 482
           GW+ TGDL Y DE+G ++VVDR+KELIKYKG+QVAPAELEALL++HPEI DA VIP PD+
Sbjct: 427 GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDK 486

Query: 483 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 538
           E G+ P+AYVVR   SSL+E+ I++F+A QV+PYKR+R+V F+SS+PK+ SGKILR++LI
Sbjct: 487 EVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 538

BLAST of Cucsa.292580 vs. TAIR10
Match: AT1G20480.1 (AT1G20480.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 419.9 bits (1078), Expect = 2.5e-117
Identity = 238/550 (43.27%), Postives = 339/550 (61.64%), Query Frame = 1

Query: 3   KSGYGRD-GIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNR--LAIVDAESSNSVSYSQ 62
           KSG+     IF S R P+  P +  L + SF+     S P+R     VDA +   +S+ +
Sbjct: 24  KSGFCESTSIFYSKREPMALPPNQFLDVTSFI----ASQPHRGKTVFVDAVTGRRLSFPE 83

Query: 63  LKALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELT 122
           L     RV+  L  LG+ K +VV+I +PNS+ F I  + V+++GAI+TT NP+ T  E++
Sbjct: 84  LWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEIS 143

Query: 123 KQVRDAKPKLVISVAELWDKV---KNLNIPTVLLDQ-QIPSAIHSPKILCFNDLVNMAGD 182
           KQ+ D++P L  +  +L  K+    N N+P VL+D   +PS  +  ++     L  M   
Sbjct: 144 KQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIET 203

Query: 183 KSGSEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVF 242
           +         V Q DTAALLYSSGTTG SKGV+L+H N IA  L+      F  E+  + 
Sbjct: 204 EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIA--LVQAYRARFGLEQRTIC 263

Query: 243 LNFLPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALA 302
              +PM H+FG        +  G T+V +PKF++ K L AVE ++ + L +VPP+V+A+ 
Sbjct: 264 T--IPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMV 323

Query: 303 K--QSLVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENP 362
                +  KY+LSS+  V +G APL RE+ E+   N P   ++QGYG+TE+  + A    
Sbjct: 324 NGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFN 383

Query: 363 AVGKRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTI 422
               +  G++G LA  VE KIV  DT + L  NQ GE+ +R P +M GYF N EAT  TI
Sbjct: 384 KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTI 443

Query: 423 DKHGWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPC 482
           D  GW+ TGDL Y D +G ++VVDR+KELIK  G+QVAPAELEALL++HPEI DA VIP 
Sbjct: 444 DSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPI 503

Query: 483 PDEEAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRR 542
           PD +AG+ P+AY+VR   S+L+E +I+ F+A QVSPYK++R+VTF++S+PK+ SGKILRR
Sbjct: 504 PDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKILRR 563

Query: 543 ELIEKVRAKI 544
           EL +   +K+
Sbjct: 564 ELTKLTTSKL 565

BLAST of Cucsa.292580 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 406.8 bits (1044), Expect = 2.2e-113
Identity = 228/543 (41.99%), Postives = 337/543 (62.06%), Query Frame = 1

Query: 11  IFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLKALAIRVSNG 70
           IFRS  P +  P   +L + ++ F  L S  ++  ++   +  S +Y +   +  RV++G
Sbjct: 34  IFRSKLPDIDIPN--HLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASG 93

Query: 71  LIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTKQVRDAKPKLV 130
           L +LGI K DV++I   NS +F   F+G   IGA+ TT NP YT  EL KQ++ +  KL+
Sbjct: 94  LYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLI 153

Query: 131 ISVAELWDKVKNL--NIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSEFPIVGVKQ 190
           I+ ++  DK+KNL  N+  +  D+  P        L F+ L+    D++      V +  
Sbjct: 154 ITHSQYVDKLKNLGENLTLITTDEPTPE-----NCLPFSTLIT--DDETNPFQETVDIGG 213

Query: 191 SDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLN-------FLPM 250
            D AAL +SSGTTG  KGV+LTH      SL+ ++ Q  +G+   ++L         LP+
Sbjct: 214 DDAAALPFSSGTTGLPKGVVLTH-----KSLITSVAQQVDGDNPNLYLKSNDVILCVLPL 273

Query: 251 FHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLVK 310
           FH++ L  +    L+ G TV+ M KF +   L  +++++VT   +VPP+V+ALAK   V 
Sbjct: 274 FHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVN 333

Query: 311 KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVAL-----ENPAVG 370
            Y+LSSV+ V SGAAPLG+EL +     +P A++ QGYGMTE   V+++     + P   
Sbjct: 334 SYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPT 393

Query: 371 KRNSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKH 430
           K  SGS GT+    E K+V ++T   L  NQ GEI +RG  +M  Y N+PEAT  TID+ 
Sbjct: 394 K--SGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEE 453

Query: 431 GWVHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDE 490
           GW+HTGD+GY DE+ ++++VDR+KE+IK+KGFQV PAELE+LL++H  I DA V+P  DE
Sbjct: 454 GWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDE 513

Query: 491 EAGEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELI 540
            AGEVP+A+VVRS  + +TEED+ +++A QV  YKRL +V F++S+PKS SGKILR++L 
Sbjct: 514 VAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLK 560

BLAST of Cucsa.292580 vs. TAIR10
Match: AT1G20500.1 (AT1G20500.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-112
Identity = 232/545 (42.57%), Postives = 342/545 (62.75%), Query Frame = 1

Query: 3   KSGYGR-DGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQLK 62
           +SG+ + +  F S R PL  P  PNLS     F +   +  + A +DA +   +++S L 
Sbjct: 13  RSGFCKSNSTFYSKRQPLSLP--PNLSRDVTTFISSQPHRGKTAFIDAATGQCLTFSDLW 72

Query: 63  ALAIRVSNGLI-QLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 122
               RV++ L  ++GI + DVVLI +PNS+   +  + V+++GA+ TT N + T  E++K
Sbjct: 73  RAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISK 132

Query: 123 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 182
           Q+ D+ P LV +  +L  K+  + I  VL D ++   + S  I     L  M   +   +
Sbjct: 133 QIADSNPTLVFTTRQLAPKLP-VAISVVLTDDEVYQELTSA-IRVVGILSEMVKKEPSGQ 192

Query: 183 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 242
                V Q DTA +LYSSGTTG SKGVI +H N  A       D   N +   +F+  +P
Sbjct: 193 RVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISD---NLKRDDIFICTVP 252

Query: 243 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS-L 302
           MFH +GL       +  G+TVV + +F L   + AVEK++ T L + PPV++A+   + L
Sbjct: 253 MFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADL 312

Query: 303 VK-KYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR 362
           +K KY+LSS+K V  G APL +E+ E      P+  ++QGY +TE+ G  A  N A   R
Sbjct: 313 IKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESR 372

Query: 363 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 422
             G+AGTL S VEA+IV  +T + +  NQ GE+ ++GP++  GYF N EAT +TI+  GW
Sbjct: 373 RYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGW 432

Query: 423 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 482
           + TGDL Y DE+G L+VVDR+KELIKYKG+QV PAELEALL++HP+ILDA VIP PD+EA
Sbjct: 433 LKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDAAVIPFPDKEA 492

Query: 483 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 542
           G+ P+AYVVR   S+L+E+ ++ FI+ QV+PYK++R+V+FI+S+PK+ SGK LR++LI+ 
Sbjct: 493 GQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASGKTLRKDLIKL 550

Query: 543 VRAKI 544
             +K+
Sbjct: 553 ATSKL 550

BLAST of Cucsa.292580 vs. NCBI nr
Match: gi|449459112|ref|XP_004147290.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis sativus])

HSP 1 Score: 1068.1 bits (2761), Expect = 4.9e-309
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsa.292580 vs. NCBI nr
Match: gi|659072020|ref|XP_008463063.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 [Cucumis melo])

HSP 1 Score: 1035.8 bits (2677), Expect = 2.7e-299
Identity = 527/543 (97.05%), Postives = 537/543 (98.90%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSG GRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESS SVSYSQL
Sbjct: 1   MEKSGCGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSYSVSYSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           KALAIR+SNGLIQLGIEKNDVV+IFAPNSVQ +ICFIGVIAIGAIVTTCNPVYTVSELTK
Sbjct: 61  KALAIRLSNGLIQLGIEKNDVVMIFAPNSVQLSICFIGVIAIGAIVTTCNPVYTVSELTK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QVRDAKPKLVISVAELWDKVK+LNIPTVLLDQ+IPSAIHS KILCFNDLVNMAGDKSGSE
Sbjct: 121 QVRDAKPKLVISVAELWDKVKDLNIPTVLLDQKIPSAIHSSKILCFNDLVNMAGDKSGSE 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FPIVGVKQSDTAALLYSSGTTGASKGVILTH NFIASSLMITMDQTF+GEEHGVFLNFLP
Sbjct: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHRNFIASSLMITMDQTFSGEEHGVFLNFLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLACITYAQLQKGNT+VSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV
Sbjct: 241 MFHVFGLACITYAQLQKGNTIVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS
Sbjct: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDK+GWVH
Sbjct: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKYGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIP PDEEAGE
Sbjct: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPYPDEEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VPIAY+VRSANSSLTEEDILKFIADQVSPYKRLRRVTFIS+VPKSVSGKILRRELIEKVR
Sbjct: 481 VPIAYIVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISNVPKSVSGKILRRELIEKVR 540

Query: 541 AKI 544
           AKI
Sbjct: 541 AKI 543

BLAST of Cucsa.292580 vs. NCBI nr
Match: gi|1009116026|ref|XP_015874554.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 803.5 bits (2074), Expect = 2.3e-229
Identity = 402/544 (73.90%), Postives = 473/544 (86.95%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV P+DPNLS+ SFLFRN  SYP++ A++DA+S+ ++S++Q 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPRDPNLSLASFLFRNSSSYPHKPALIDADSNETLSFAQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K++ I+VS+GL+ LGI+KNDVVLIFAPNS+QF ICF+G +AIGAI TT NP+YTVSEL+K
Sbjct: 61  KSMVIKVSHGLLHLGIKKNDVVLIFAPNSIQFPICFLGTVAIGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIH-SPKILCFNDLVNMAGDKSGS 180
           QV+D+ PKLV++V EL++KVK+ N+P VLL  +  S +  S KIL F+DLV+++G  S S
Sbjct: 121 QVKDSNPKLVVTVPELFEKVKDFNLPVVLLGSKDSSRVALSSKILNFHDLVSLSG--SAS 180

Query: 181 EFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFL 240
           EFP+V VKQ+DTAALLYSSGTTGASKGVILTH NFIA+SLM+TMDQ   GE+  +FL FL
Sbjct: 181 EFPMVNVKQTDTAALLYSSGTTGASKGVILTHRNFIAASLMVTMDQEVAGEKDNLFLCFL 240

Query: 241 PMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSL 300
           PMFHVFGLA ITY+QLQ+GNTVVSM +F+ EK L  VEKYK+T LWVVPP+VLALAKQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNTVVSMGRFDFEKILKVVEKYKITHLWVVPPIVLALAKQSV 300

Query: 301 VKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRN 360
           VKKY+LSS+K VGSGAAPLG++LMEECA  IP   V QG+GMTETCG++++ENP VGKR 
Sbjct: 301 VKKYDLSSLKHVGSGAAPLGKDLMEECAKVIPGGAVFQGFGMTETCGIISVENPQVGKRY 360

Query: 361 SGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWV 420
           SGSAG L SGVEA+IVSV++LKPLPPNQ GEI VRGPNMM GY+N P+ATK TIDK GWV
Sbjct: 361 SGSAGMLVSGVEAQIVSVESLKPLPPNQLGEIWVRGPNMMQGYYNKPQATKLTIDKQGWV 420

Query: 421 HTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAG 480
           HTGDLGYFDENGQL+VVDR+KELIKYKGFQVAPAELE LLVSHPEILDAVVIP PD EAG
Sbjct: 421 HTGDLGYFDENGQLFVVDRLKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAG 480

Query: 481 EVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKV 540
           EVP AYVVR  NSSLTEED+  FIA QV+P+KRLRRVTFI+SVPKS SGKILRRELI+KV
Sbjct: 481 EVPAAYVVRKPNSSLTEEDVQNFIAKQVAPFKRLRRVTFINSVPKSASGKILRRELIQKV 540

Query: 541 RAKI 544
           RAKI
Sbjct: 541 RAKI 542

BLAST of Cucsa.292580 vs. NCBI nr
Match: gi|927093280|gb|ALD83616.1| (4-coumarate-CoA ligase 7-2 [Morus alba])

HSP 1 Score: 801.6 bits (2069), Expect = 8.6e-229
Identity = 398/543 (73.30%), Postives = 461/543 (84.90%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDGIFRSLRPPLV PKDPNLS +SFLFRN  SYPN+ A+++A+S   +S+SQ 
Sbjct: 1   MEKSGYGRDGIFRSLRPPLVLPKDPNLSAVSFLFRNSSSYPNKPALINADSGEILSFSQF 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K+   +VS+G + LGI+KNDVVLIFAPNS+ F +CF+G+IA+GAI TT NP+YTVSEL+K
Sbjct: 61  KSTVAKVSHGFLHLGIKKNDVVLIFAPNSIHFPVCFLGIIAVGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIPSAIHSPKILCFNDLVNMAGDKSGSE 180
           QV+D+ PKL+I+V ELW+KVK  N+PTV+L  +  S     K++ FNDLVN++G  S S+
Sbjct: 121 QVKDSNPKLIITVPELWEKVKGFNLPTVMLSAEPKSV--PKKVVTFNDLVNLSG--SASD 180

Query: 181 FPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNFLP 240
           FP V VK+SDTA LLYSSGTTG SKGV+LTHGNFIA+SLM+ MDQ    E H VFL  LP
Sbjct: 181 FPSVKVKRSDTAVLLYSSGTTGTSKGVVLTHGNFIAASLMVAMDQDLAKEMHNVFLCVLP 240

Query: 241 MFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQSLV 300
           MFHVFGLA ITY QLQ+GN +VSM KF+LEK L  VEKYK+T LW VPP+VLALAK S+V
Sbjct: 241 MFHVFGLAVITYTQLQRGNAIVSMAKFDLEKILMTVEKYKITHLWFVPPIVLALAKNSVV 300

Query: 301 KKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKRNS 360
           KKYNLSS+K +GSGAAPLG+ELMEECA  IP  VVIQGYGMTETCG+V++E+   GKRN+
Sbjct: 301 KKYNLSSLKHIGSGAAPLGKELMEECAKIIPQGVVIQGYGMTETCGIVSVEDTRGGKRNT 360

Query: 361 GSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGWVH 420
           GSAGTL SGVEA+IV VDTLKPLPPNQ GEI VRGPNMM GY NNP+ATK TIDK GWVH
Sbjct: 361 GSAGTLVSGVEAQIVGVDTLKPLPPNQLGEIWVRGPNMMQGYLNNPQATKSTIDKKGWVH 420

Query: 421 TGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEAGE 480
           TGDLGYFDE+G L+VVDRIKELIKYKGFQVAPAELE LLVSHPEI DAVVIP PD EAGE
Sbjct: 421 TGDLGYFDEDGHLFVVDRIKELIKYKGFQVAPAELEGLLVSHPEIFDAVVIPFPDAEAGE 480

Query: 481 VPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEKVR 540
           VP+AYVVRS NSSLTEED+ KFIA+QV+P+KRLR+VTFI+SVPKS SGKILRRELIEKVR
Sbjct: 481 VPVAYVVRSPNSSLTEEDVQKFIANQVAPFKRLRKVTFINSVPKSASGKILRRELIEKVR 539

Query: 541 AKI 544
           +KI
Sbjct: 541 SKI 539

BLAST of Cucsa.292580 vs. NCBI nr
Match: gi|470110788|ref|XP_004291645.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 [Fragaria vesca subsp. vesca])

HSP 1 Score: 799.7 bits (2064), Expect = 3.3e-228
Identity = 398/545 (73.03%), Postives = 471/545 (86.42%), Query Frame = 1

Query: 1   MEKSGYGRDGIFRSLRPPLVFPKDPNLSMISFLFRNLLSYPNRLAIVDAESSNSVSYSQL 60
           MEKSGYGRDG+FRSLRPPLV P+DPN +M+SFLF N  SYPN+ A++DAESS ++S+SQL
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPRDPNFTMVSFLFNNSSSYPNKPALIDAESSETLSFSQL 60

Query: 61  KALAIRVSNGLIQLGIEKNDVVLIFAPNSVQFTICFIGVIAIGAIVTTCNPVYTVSELTK 120
           K++ I+V++GL+ LG++KNDVVLIFAPNS+QF +CF+G+IAIGAI TT NP+YTVSEL+K
Sbjct: 61  KSMVIKVAHGLVHLGVKKNDVVLIFAPNSIQFPVCFLGIIAIGAIATTSNPLYTVSELSK 120

Query: 121 QVRDAKPKLVISVAELWDKVKNLNIPTVLLDQQIP-SAIHSP-KILCFNDLVNMAGDKSG 180
           QV+D+ PKLVI+V ELW+KVK LN+P V+L  +   S I S  K++ F+DLV + G  + 
Sbjct: 121 QVKDSNPKLVITVPELWEKVKGLNLPAVILGPEGSCSQIESTSKMVGFHDLVKLCG--TV 180

Query: 181 SEFPIVGVKQSDTAALLYSSGTTGASKGVILTHGNFIASSLMITMDQTFNGEEHGVFLNF 240
           S+FP V VKQ+DTA LLYSSGTTG SKGV+LTH NFIASS+M+T DQ   GE H VFL  
Sbjct: 181 SDFPSVNVKQTDTAVLLYSSGTTGLSKGVVLTHRNFIASSVMVTHDQELTGEMHRVFLCV 240

Query: 241 LPMFHVFGLACITYAQLQKGNTVVSMPKFNLEKALWAVEKYKVTDLWVVPPVVLALAKQS 300
           LP+FHVFGLA ITYAQL+KGN VVSM +FNLEK L  VEKYKVT LWVVPP++LAL+K S
Sbjct: 241 LPIFHVFGLAIITYAQLRKGNAVVSMSRFNLEKILMTVEKYKVTHLWVVPPIILALSKDS 300

Query: 301 LVKKYNLSSVKRVGSGAAPLGRELMEECANNIPSAVVIQGYGMTETCGVVALENPAVGKR 360
           +VKKYNLSS+K++GSGAAPLG+ELMEECA   P  +V+QGYGMTETCG+V++EN  VG R
Sbjct: 301 VVKKYNLSSLKQIGSGAAPLGKELMEECAKVFPQGLVVQGYGMTETCGIVSVENTLVGPR 360

Query: 361 NSGSAGTLASGVEAKIVSVDTLKPLPPNQYGEIHVRGPNMMLGYFNNPEATKQTIDKHGW 420
           +SGSAGTL SGVE +IVSVDTLKPLPP Q GEI VRGPNMM+GYFNNP+AT+QTIDK+GW
Sbjct: 361 HSGSAGTLVSGVECQIVSVDTLKPLPPKQLGEIWVRGPNMMIGYFNNPQATQQTIDKNGW 420

Query: 421 VHTGDLGYFDENGQLYVVDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPCPDEEA 480
           VHTGDLGYFDE+GQL+VVDRIKELIKYKG+QVAPAELE LLVSHPEILDAVVIP PD EA
Sbjct: 421 VHTGDLGYFDESGQLFVVDRIKELIKYKGYQVAPAELEGLLVSHPEILDAVVIPFPDAEA 480

Query: 481 GEVPIAYVVRSANSSLTEEDILKFIADQVSPYKRLRRVTFISSVPKSVSGKILRRELIEK 540
           GEVP+AYVVRS N+SLTEEDI  FIA QV+ +KRLRRVTFI++VPKS SGKILRRELIEK
Sbjct: 481 GEVPVAYVVRSPNTSLTEEDIKSFIASQVASFKRLRRVTFINTVPKSASGKILRRELIEK 540

Query: 541 VRAKI 544
           VR+KI
Sbjct: 541 VRSKI 543