Lsi02G004560 (gene) Bottle gourd (USVL1VR-Ls)

NameLsi02G004560
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls))
DescriptionPectate lyase
Locationchr02 : 3822873 .. 3826272 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTTCCTCTTTCTCTTCTCCGTCTCTCTCTCCGCCGCTCTCACCGCCGCTCCTGCGGCCGTTCCCAACCCCGAACTCGTCGTACAAGAAGTACACAGGTAAAAAAAAACACACAAACATTCCCATTACTCTGTTTTTCTTGTTTCGTTCTCTGTTTTGAAAATGTGCGTCTCATTTTGGGTTTCAGGAGCATAGTCAATGCGACGAAGAGGAGGAATTTAGGGTATCTCTCATGTGGGACTGGAAATCCAATCGACGATTGCTGGCGGTGCGACCCCAATTGGGAGAAAAATCGTCAGAGACTAGCTGACTGTGGGATTGGATTCGGTAAAAACGCCATTGGCGGTCGCGACGGCCAAATCTACGTCGTCACTGACGCCGGCGACGACGATCCAGTCAACCCTAAACCAGGAACTCTCCGTTACGCAGTAATCCAAGACGAACCCCTCTGGATTATCTTCGCTCGCGATATGGTGATTAGATTGAAAGAAGAACTGATTATGAATTCCTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACGAACATTATAATCCACGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACAGACGTGAGAGATTCCCCTCGACATTTCGGATTCAGAACCATATCGGACGGCGACGGCGTTTCAATCTTCGGCGGCAGCCATGTTTGGGTTGATCACTGCTCTTTATCGAACTGTAACGATGGATTAATCGACGCCATTCACGGGTCTACAGCGATTACGATTTCAAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTACACTCAAGACAAGAATATGCAAGTCACCATTGCTTTCAACCATTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGGTAAATATTGAATCCCATTTTCTCTGTGTTTGTGTGTGTTCTGTTCTTGAAATTGAAATTGGAATTTGGGGGATTTTGATTTGCAGATGCAGACATGGGTATTTTCATGTGGTGAATAATGACTATACGCATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACTATCAACAGTCAAGGAAACAGATTTGTTGCACCAAATGACAGATTCAGCAAAGAGGTAAGAAGATAAAACAATACACAATACTTATTTTAAGGGTTCATTTGTGTTTATTTGATATTTTTAAAAAATTGTTTAATGAATTTTCGAATCTTTCGTTAAATTGCAATTTAGTCCTACATATAGGAGAAAATTAAAATTTAATCTATATAGTTTATAATTAGAATTTAGTCCTTTTGACAATCTAGAGACTATTTATGAGAATTGATAGACTAAATAGATTATTTATAAGATTTTATCAAACTATAAAAACTAACTCATAATTTTTTTTTTCAAATTATAAGAACCAAATTTATAATTTTACCTAAACTTTTAATAATTTTTTTTGTTTAATTATACGGAATATATCACTTAATTTAAAAAATATATCTCTAAAAAAAATAGATATTTTTTAAATTCAAATATTAAATAAACATAGATCTAAGGATATGTTTGGTAGAAGGAGAAGGAGTAGGAGTTGGAGAGTAGAGTTGTTTTGTTTGAAGATTTTGTTGACCATACAACCGGAAAACATCAATTTTATAATTTATTAATTTTTTACCCGATTAGATAAAACTTAAAACTTAAAACTGAATGAGTTGAGGGATTAAAAATTTAAATGTTTAAGAGTTGAAGGACTAAATTTGTAATTTTATTATTTGATTGTGATGGGTAAAAAATAAGGTTATCATTATGGATTTGTGATCTTTATGGATATTGGTGAATTGCAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGATCAGAAGGGGATCTGATGCTAAACGGTGCGTTTTTCACTGCCTCCGGCGCCGGAGCATCGTCCAGTTACGCTAGAGCATCGAGCTTAGGGGCGAGACCGTCGTCGCTCGTCGGTACGATTACGACCAACGCGGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGATAATTACTCCTCTACCCAATTTGAAACCAAAAAAGAAAAAAGAACTGAAATTTGAAAGTGTAGTAGCTAGTTCTAGTTGGTTGGTTGATTTTCTTAAAAGTTTAAAATTATTGATTTTATGCAATGGTAATTTGAGGGAAAATCAGTGGGAAATGGAGGGAAAGTAAAAGAGGGAAAAAAGAAAAGAAAAGAGAGCTGAGAAAAGACACTGAACCAATAGGGGTCAGTCAATCTGCTTCTATCAGTATATCTTTTTTAGCCTTTTTCTTTATCTCCTTCCATTTTTTTTTTTGTTTTTGTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTGGGTTGTTTTTATAATTATTACAATGTACAACCTCCGCATGTAATTCTTGGAACCATTGGGGCTCTCCTTTTAAGAACCTTAATGGTCTCAAATCAAGTCAAATGCAGAAGAGTTGATATTTGATATACT

mRNA sequence

ATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTTCCTCTTTCTCTTCTCCGTCTCTCTCTCCGCCGCTCTCACCGCCGCTCCTGCGGCCGTTCCCAACCCCGAACTCGTCGTACAAGAAGTACACAGGAGCATAGTCAATGCGACGAAGAGGAGGAATTTAGGGTATCTCTCATGTGGGACTGGAAATCCAATCGACGATTGCTGGCGGTGCGACCCCAATTGGGAGAAAAATCGTCAGAGACTAGCTGACTGTGGGATTGGATTCGGTAAAAACGCCATTGGCGGTCGCGACGGCCAAATCTACGTCGTCACTGACGCCGGCGACGACGATCCAGTCAACCCTAAACCAGGAACTCTCCGTTACGCAGTAATCCAAGACGAACCCCTCTGGATTATCTTCGCTCGCGATATGGTGATTAGATTGAAAGAAGAACTGATTATGAATTCCTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACGAACATTATAATCCACGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACAGACGTGAGAGATTCCCCTCGACATTTCGGATTCAGAACCATATCGGACGGCGACGGCGTTTCAATCTTCGGCGGCAGCCATGTTTGGGTTGATCACTGCTCTTTATCGAACTGTAACGATGGATTAATCGACGCCATTCACGGGTCTACAGCGATTACGATTTCAAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTACACTCAAGACAAGAATATGCAAGTCACCATTGCTTTCAACCATTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGATGCAGACATGGGTATTTTCATGTGGTGAATAATGACTATACGCATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACTATCAACAGTCAAGGAAACAGATTTGTTGCACCAAATGACAGATTCAGCAAAGAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGATCAGAAGGGGATCTGATGCTAAACGGTGCGTTTTTCACTGCCTCCGGCGCCGGAGCATCGTCCAGTTACGCTAGAGCATCGAGCTTAGGGGCGAGACCGTCGTCGCTCGTCGGTACGATTACGACCAACGCGGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGATAATTACTCCTCTACCCAATTTGAAACCAAAAAAGAAAAAAGAACTGAAATTTGAAAGTGTAGTAGCTAGTTCTAGTTGGTTGGTTGATTTTCTTAAAAGTTTAAAATTATTGATTTTATGCAATGGTAATTTGAGGGAAAATCAGTGGGAAATGGAGGGAAAGTAAAAGAGGGAAAAAAGAAAAGAAAAGAGAGCTGAGAAAAGACACTGAACCAATAGGGGTCAGTCAATCTGCTTCTATCAGTATATCTTTTTTAGCCTTTTTCTTTATCTCCTTCCATTTTTTTTTTTGTTTTTGTTTTTTTTTTTTTTTTGGTTTTGGGTTGTTTTTATAATTATTACAATGTACAACCTCCGCATGTAATTCTTGGAACCATTGGGGCTCTCCTTTTAAGAACCTTAATGGTCTCAAATCAAGTCAAATGCAGAAGAGTTGATATTTGATATACT

Coding sequence (CDS)

ATGGCACCGCCTACAGTCCCTCTCTCTCTCCTCTTCCTCTTTCTCTTCTCCGTCTCTCTCTCCGCCGCTCTCACCGCCGCTCCTGCGGCCGTTCCCAACCCCGAACTCGTCGTACAAGAAGTACACAGGAGCATAGTCAATGCGACGAAGAGGAGGAATTTAGGGTATCTCTCATGTGGGACTGGAAATCCAATCGACGATTGCTGGCGGTGCGACCCCAATTGGGAGAAAAATCGTCAGAGACTAGCTGACTGTGGGATTGGATTCGGTAAAAACGCCATTGGCGGTCGCGACGGCCAAATCTACGTCGTCACTGACGCCGGCGACGACGATCCAGTCAACCCTAAACCAGGAACTCTCCGTTACGCAGTAATCCAAGACGAACCCCTCTGGATTATCTTCGCTCGCGATATGGTGATTAGATTGAAAGAAGAACTGATTATGAATTCCTTCAAAACCATCGACGGCCGTGGCGCTAGTGTCCATATCGCCGGTGGGCCTTGCATTACGATTCAGTACGTGACGAACATTATAATCCACGGTCTCAACATTCACGACTGTAAACCAGGGGGAAACACAGACGTGAGAGATTCCCCTCGACATTTCGGATTCAGAACCATATCGGACGGCGACGGCGTTTCAATCTTCGGCGGCAGCCATGTTTGGGTTGATCACTGCTCTTTATCGAACTGTAACGATGGATTAATCGACGCCATTCACGGGTCTACAGCGATTACGATTTCAAACAATTACATGACGCATCATGATAAAGTGATGTTGTTGGGGCACAGTGATTCGTACACTCAAGACAAGAATATGCAAGTCACCATTGCTTTCAACCATTTTGGTGAAGGTCTTGTTCAAAGAATGCCCAGATGCAGACATGGGTATTTTCATGTGGTGAATAATGACTATACGCATTGGGAAATGTACGCCATTGGAGGAAGTGCTGCTCCAACTATCAACAGTCAAGGAAACAGATTTGTTGCACCAAATGACAGATTCAGCAAAGAGGTGACAAAATACGAGGATGCACCAGAGAGTGAGTGGAAGAATTGGCATTGGAGATCAGAAGGGGATCTGATGCTAAACGGTGCGTTTTTCACTGCCTCCGGCGCCGGAGCATCGTCCAGTTACGCTAGAGCATCGAGCTTAGGGGCGAGACCGTCGTCGCTCGTCGGTACGATTACGACCAACGCGGGTGCACTGAACTGCAGAAAGGGCTCCCGCTGCTGA

Protein sequence

MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
BLAST of Lsi02G004560 vs. Swiss-Prot
Match: PLY18_ARATH (Probable pectate lyase 18 OS=Arabidopsis thaliana GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 689.5 bits (1778), Expect = 2.2e-197
Identity = 328/402 (81.59%), Postives = 365/402 (90.80%), Query Frame = 1

Query: 11  LFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSI-VNATKRRNLGYLSCGTGNPIDDCW 70
           LF+ + S  L A L  + + VP+PE VV+EVH+SI  +   RR LGYLSC TGNPIDDCW
Sbjct: 8   LFITIVSFLLYAPLFLS-SPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCW 67

Query: 71  RCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEP 130
           RCDP+WE++RQRLADC IGFGKNAIGGRDG+IYVVTD+G+D+PV+PKPGTLR+AV+QDEP
Sbjct: 68  RCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEP 127

Query: 131 LWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKP 190
           LWIIF RDM I+LKEELIMNSFKTIDGRGASVHI+GGPCITIQYVTNIIIHG++IHDCK 
Sbjct: 128 LWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQ 187

Query: 191 GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISN 250
           GGN  VR SPRHFG+RTISDGDGVSIFGGSHVWVDHCS SNC DGLIDAI GSTAIT+SN
Sbjct: 188 GGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSN 247

Query: 251 NYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 310
           N+MTHHDKVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE
Sbjct: 248 NHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 307

Query: 311 MYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFT 370
           MYAIGGSA PTINSQGNRF+APN RFSKEVTK+EDAPESEWK W+WRS GDL+LNGAFFT
Sbjct: 308 MYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFT 367

Query: 371 ASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
            SG  ASSSYA+ASSLGA+PSSLVG +T+ +GALNCRKGSRC
Sbjct: 368 PSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Lsi02G004560 vs. Swiss-Prot
Match: PLY22_ARATH (Probable pectate lyase 22 OS=Arabidopsis thaliana GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 8.5e-197
Identity = 326/406 (80.30%), Postives = 362/406 (89.16%), Query Frame = 1

Query: 8   LSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDD 67
           LS   +F   +  SA  ++ P  V +PELVV+EVHR I  +  RR LG+ SCG+GNPIDD
Sbjct: 29  LSFALIFFCCILFSALASSLP--VSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDD 88

Query: 68  CWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQD 127
           CWRCD +WEKNR+RLADCGIGFGKNAIGGRDG+IYVVTD G+DDPVNP+PGTLRYAVIQD
Sbjct: 89  CWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQD 148

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIF RDM I+LKEELIMNSFKT+DGRGASVHI+GGPCITIQYVTNIIIHGL+IHDC
Sbjct: 149 EPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDC 208

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           K GGNT VRDSP H+G+RT+SDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAI GSTAITI
Sbjct: 209 KQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITI 268

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 269 SNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 328

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+D  SKEVTK+EDAPE EW+NW+WRSEGDL+LNGAF
Sbjct: 329 WEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAF 388

Query: 368 FTASGAG--ASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FT SGAG   SSSY++ASSL ARPSS VG IT  +GAL+C++GS C
Sbjct: 389 FTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Lsi02G004560 vs. Swiss-Prot
Match: PLY5_ARATH (Probable pectate lyase 5 OS=Arabidopsis thaliana GN=At1g67750 PE=2 SV=2)

HSP 1 Score: 681.4 bits (1757), Expect = 6.1e-195
Identity = 333/403 (82.63%), Postives = 357/403 (88.59%), Query Frame = 1

Query: 11  LFLFLFSVSLSAALTAAPAAVP--NPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDC 70
           L L LFS  L        A+ P   PELVVQEV+  I NA+ RRNLG LSCGTGNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKI-NAS-RRNLGVLSCGTGNPIDDC 67

Query: 71  WRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDE 130
           WRCDP WEKNRQRLADC IGFGK+AIGGRDG+IYVVTD+ D D VNPKPGTLR+AVIQDE
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 131 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 190
           PLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGG CIT+QYVTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 191 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 250
             GN  VRDSP H+G+RT SDGD VSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAITIS
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 251 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 310
           NNY++HH+KVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 311 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 370
           +MYAIGGSAAPTINSQGNRF+APND   KEVTKYEDAP S+WK W+WRSEGDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 371 TASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           T SG GASSSYA+ASSL ARPSSLV ++T+NAGAL CRKGSRC
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of Lsi02G004560 vs. Swiss-Prot
Match: PLY11_ARATH (Putative pectate lyase 11 OS=Arabidopsis thaliana GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 653.3 bits (1684), Expect = 1.8e-186
Identity = 307/401 (76.56%), Postives = 350/401 (87.28%), Query Frame = 1

Query: 12  FLFLFSVSLSAALTAA-PAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDCWR 71
           FL L ++  + A +++ P  V +P LVV +V+RS+ NA+ RR+L YLSC TGNPIDDCWR
Sbjct: 13  FLLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNAS-RRSLAYLSCRTGNPIDDCWR 72

Query: 72  CDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEPL 131
           CDPNWE NRQRLADC IGFGKNAIGGR G+IYVVTD  +DDPVNP+PGTLRYAV Q+EPL
Sbjct: 73  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 132

Query: 132 WIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPG 191
           WIIF RDMVIRLK+ELI+ SFKTIDGRG+SVHI  GPC+ I Y TNIIIHG+NIHDCKPG
Sbjct: 133 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 192

Query: 192 GNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNN 251
               ++D P H G+   SDGD V+IFGG HVW+DHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 193 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 252

Query: 252 YMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 311
           +MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 253 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 312

Query: 312 YAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTA 371
           YAIGGSA+PTI SQGNRF+APN RF+KEVTK+EDAPES+W++W+WRSEGD++LNGA+F  
Sbjct: 313 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 372

Query: 372 SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           SGA A S+YARASSL ARPSSLVG+ITT AG L+CR+G RC
Sbjct: 373 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Lsi02G004560 vs. Swiss-Prot
Match: PLY_ZINVI (Pectate lyase OS=Zinnia violacea PE=1 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 1.3e-184
Identity = 310/403 (76.92%), Postives = 353/403 (87.59%), Query Frame = 1

Query: 9   SLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDC 68
           ++L L LF  SL+ A +++P+  P+   +V EVH+SI NA+ RRNLGYLSCGTGNPIDDC
Sbjct: 4   TILPLILFISSLAIA-SSSPSRTPHA--IVNEVHKSI-NAS-RRNLGYLSCGTGNPIDDC 63

Query: 69  WRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDE 128
           WRCDPNW  NRQRLADC IGFGKNA+GGR+G+IYVVTD G+DDPVNP PGTLRYAVIQDE
Sbjct: 64  WRCDPNWANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDE 123

Query: 129 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 188
           PLWIIF RDMVI+L++EL+MNS KTIDGRG +VHI  GPCITI Y +NIIIHG++IHDCK
Sbjct: 124 PLWIIFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCK 183

Query: 189 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 248
             GN ++R+SP H G+ T SDGDG+SIF    +W+DH SLSNC+DGLIDAIHGSTAITIS
Sbjct: 184 QAGNGNIRNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITIS 243

Query: 249 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 308
           NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 244 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 303

Query: 309 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 368
           EMYAIGGSA+PTI SQGNRF+APN RF KEVTK+E+APESEWKNW+WRSEGDLMLNGA+F
Sbjct: 304 EMYAIGGSASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYF 363

Query: 369 TASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
             SG  A+SS+ARASSL  RPS+LV ++T +AGAL CRKGSRC
Sbjct: 364 RESGGRAASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of Lsi02G004560 vs. TrEMBL
Match: A0A0A0LU82_CUCSA (Pectate lyase OS=Cucumis sativus GN=Csa_1G049960 PE=3 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 1.7e-236
Identity = 398/413 (96.37%), Postives = 405/413 (98.06%), Query Frame = 1

Query: 1   MAPPTVPLSL--LFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLS 60
           MAPP +PLSL  LFLFLFS+SLSAAL+  PAAV NPELVVQEVHRSI+NATKRRNLGYLS
Sbjct: 1   MAPPALPLSLLSLFLFLFSISLSAALSPNPAAVSNPELVVQEVHRSIINATKRRNLGYLS 60

Query: 61  CGTGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPG 120
           CGTGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPG
Sbjct: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPG 120

Query: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180
           TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII
Sbjct: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180

Query: 181 IHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240
           IHGLNIHDCK GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA
Sbjct: 181 IHGLNIHDCKQGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240

Query: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300
           IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF
Sbjct: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300

Query: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360
           HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE
Sbjct: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360

Query: 361 GDLMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GDLMLNGA+FTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 GDLMLNGAYFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 413

BLAST of Lsi02G004560 vs. TrEMBL
Match: W9RSL5_9ROSA (Pectate lyase OS=Morus notabilis GN=L484_018936 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 7.2e-211
Identity = 354/404 (87.62%), Postives = 376/404 (93.07%), Query Frame = 1

Query: 8   LSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDD 67
           L  L +FLFS  +    T   +  P+PE VV EVHRSI  +  RRNLGYLSCGTGNPIDD
Sbjct: 10  LLTLIVFLFSFQIP---TLHSSPTPDPERVVGEVHRSINASVSRRNLGYLSCGTGNPIDD 69

Query: 68  CWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQD 127
           CWRCDPNWEKNRQRLADC IGFGKNA+GGRDG+IYVVTD+ D+D VNPKPGTLRYAVIQD
Sbjct: 70  CWRCDPNWEKNRQRLADCAIGFGKNAVGGRDGKIYVVTDSSDNDVVNPKPGTLRYAVIQD 129

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGGPCITIQ+VTNIIIHGLNIHDC
Sbjct: 130 EPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGLNIHDC 189

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           KPGGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAIT+
Sbjct: 190 KPGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHCSLSNCQDGLIDAIHGSTAITL 249

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 250 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 309

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+ RFSKEVTK+EDAPESEWKNW+WRSEGDLM+NGAF
Sbjct: 310 WEMYAIGGSANPTINSQGNRFLAPDKRFSKEVTKHEDAPESEWKNWNWRSEGDLMVNGAF 369

Query: 368 FTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FTASGAGASSSYA+ASSLGARPS+LVGTITTNAGAL+CRKGSRC
Sbjct: 370 FTASGAGASSSYAKASSLGARPSTLVGTITTNAGALSCRKGSRC 410

BLAST of Lsi02G004560 vs. TrEMBL
Match: D3IZZ1_GOSHE (Pectate lyase OS=Gossypium herbaceum GN=Pel PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 2.7e-210
Identity = 359/411 (87.35%), Postives = 385/411 (93.67%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MA P++PL  LFLF F + L   ++++P  V +PELVVQ+VHR+I NA+ RRNLGYLSCG
Sbjct: 7   MAIPSLPL--LFLFTFLLLLPLLISSSP--VQDPELVVQDVHRAI-NAS-RRNLGYLSCG 66

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCDPNWE NRQ+LADC IGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPGTL
Sbjct: 67  TGNPIDDCWRCDPNWETNRQKLADCAIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPGTL 126

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           R+AVIQDEPLWIIFARDM I+LKEELIMNSFKTIDGRGASVHIAGGPCIT+QYVTNIIIH
Sbjct: 127 RHAVIQDEPLWIIFARDMTIQLKEELIMNSFKTIDGRGASVHIAGGPCITVQYVTNIIIH 186

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDH SLSNC DGLIDAIH
Sbjct: 187 GLNIHDCKQGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHNSLSNCKDGLIDAIH 246

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 247 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 306

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSA PTINSQGNRF APN+RFSKEVTK+EDAPESEWK+W+WRSEGD
Sbjct: 307 VNNDYTHWEMYAIGGSANPTINSQGNRFTAPNNRFSKEVTKHEDAPESEWKSWNWRSEGD 366

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFF ASGAGASSSYA+ASSLGARPSSLV TITTNAGALNC+KGSRC
Sbjct: 367 LMVNGAFFIASGAGASSSYAKASSLGARPSSLVATITTNAGALNCKKGSRC 411

BLAST of Lsi02G004560 vs. TrEMBL
Match: I1T4H1_GOSAI (Pectate lyase OS=Gossypium aridum PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 4.7e-210
Identity = 359/411 (87.35%), Postives = 385/411 (93.67%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MA P++PL  LFLF F + L   ++++P  V +PELVVQ+VHR+I NA+ RRNLGYLSCG
Sbjct: 7   MAIPSLPL--LFLFTFLLLLPLLISSSP--VQDPELVVQDVHRAI-NAS-RRNLGYLSCG 66

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCDPNWE NRQ+LADC IGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPGTL
Sbjct: 67  TGNPIDDCWRCDPNWETNRQKLADCAIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPGTL 126

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           R+AVIQDEPLWIIFARDM I+LKEELIMNSFKTIDGRGASVHIAGGPCIT+QYVTNIIIH
Sbjct: 127 RHAVIQDEPLWIIFARDMTIQLKEELIMNSFKTIDGRGASVHIAGGPCITVQYVTNIIIH 186

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDH SLSNC DGLIDAIH
Sbjct: 187 GLNIHDCKQGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHNSLSNCKDGLIDAIH 246

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 247 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 306

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSA PTINSQGNRF AP++RFSKEVTK+EDAPESEWK+W+WRSEGD
Sbjct: 307 VNNDYTHWEMYAIGGSANPTINSQGNRFTAPDNRFSKEVTKHEDAPESEWKSWNWRSEGD 366

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFF ASGAGASSSYARASSLGARPSSLV TITTNAGALNC+KGSRC
Sbjct: 367 LMVNGAFFIASGAGASSSYARASSLGARPSSLVATITTNAGALNCKKGSRC 411

BLAST of Lsi02G004560 vs. TrEMBL
Match: I1T4F2_9ROSI (Pectate lyase OS=Gossypium laxum PE=3 SV=1)

HSP 1 Score: 738.4 bits (1905), Expect = 4.7e-210
Identity = 359/411 (87.35%), Postives = 385/411 (93.67%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MA P++PL  LFLF F + L   ++++P  V +PELVVQ+VHR+I NA+ RRNLGYLSCG
Sbjct: 7   MAIPSLPL--LFLFTFLLLLPLLISSSP--VQDPELVVQDVHRAI-NAS-RRNLGYLSCG 66

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCDPNWE NRQ+LADC IGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPGTL
Sbjct: 67  TGNPIDDCWRCDPNWETNRQKLADCAIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPGTL 126

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           R+AVIQDEPLWIIFARDM I+LKEELIMNSFKTIDGRGASVHIAGGPCIT+QYVTNIIIH
Sbjct: 127 RHAVIQDEPLWIIFARDMTIQLKEELIMNSFKTIDGRGASVHIAGGPCITVQYVTNIIIH 186

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDH SLSNC DGLIDAIH
Sbjct: 187 GLNIHDCKQGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHNSLSNCKDGLIDAIH 246

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 247 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 306

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSA PTINSQGNRF AP++RFSKEVTK+EDAPESEWK+W+WRSEGD
Sbjct: 307 VNNDYTHWEMYAIGGSANPTINSQGNRFTAPDNRFSKEVTKHEDAPESEWKSWNWRSEGD 366

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFF ASGAGASSSYARASSLGARPSSLV TITTNAGALNC+KGSRC
Sbjct: 367 LMVNGAFFIASGAGASSSYARASSLGARPSSLVATITTNAGALNCKKGSRC 411

BLAST of Lsi02G004560 vs. TAIR10
Match: AT4G24780.1 (AT4G24780.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 689.5 bits (1778), Expect = 1.3e-198
Identity = 328/402 (81.59%), Postives = 365/402 (90.80%), Query Frame = 1

Query: 11  LFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSI-VNATKRRNLGYLSCGTGNPIDDCW 70
           LF+ + S  L A L  + + VP+PE VV+EVH+SI  +   RR LGYLSC TGNPIDDCW
Sbjct: 8   LFITIVSFLLYAPLFLS-SPVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDCW 67

Query: 71  RCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEP 130
           RCDP+WE++RQRLADC IGFGKNAIGGRDG+IYVVTD+G+D+PV+PKPGTLR+AV+QDEP
Sbjct: 68  RCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDEP 127

Query: 131 LWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKP 190
           LWIIF RDM I+LKEELIMNSFKTIDGRGASVHI+GGPCITIQYVTNIIIHG++IHDCK 
Sbjct: 128 LWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCKQ 187

Query: 191 GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISN 250
           GGN  VR SPRHFG+RTISDGDGVSIFGGSHVWVDHCS SNC DGLIDAI GSTAIT+SN
Sbjct: 188 GGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLSN 247

Query: 251 NYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 310
           N+MTHHDKVMLLGHSD+Y++DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE
Sbjct: 248 NHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWE 307

Query: 311 MYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFT 370
           MYAIGGSA PTINSQGNRF+APN RFSKEVTK+EDAPESEWK W+WRS GDL+LNGAFFT
Sbjct: 308 MYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFFT 367

Query: 371 ASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
            SG  ASSSYA+ASSLGA+PSSLVG +T+ +GALNCRKGSRC
Sbjct: 368 PSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Lsi02G004560 vs. TAIR10
Match: AT5G63180.1 (AT5G63180.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 687.6 bits (1773), Expect = 4.8e-198
Identity = 326/406 (80.30%), Postives = 362/406 (89.16%), Query Frame = 1

Query: 8   LSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDD 67
           LS   +F   +  SA  ++ P  V +PELVV+EVHR I  +  RR LG+ SCG+GNPIDD
Sbjct: 29  LSFALIFFCCILFSALASSLP--VSDPELVVEEVHRKINESISRRKLGFFSCGSGNPIDD 88

Query: 68  CWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQD 127
           CWRCD +WEKNR+RLADCGIGFGKNAIGGRDG+IYVVTD G+DDPVNP+PGTLRYAVIQD
Sbjct: 89  CWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQD 148

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIF RDM I+LKEELIMNSFKT+DGRGASVHI+GGPCITIQYVTNIIIHGL+IHDC
Sbjct: 149 EPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDC 208

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           K GGNT VRDSP H+G+RT+SDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAI GSTAITI
Sbjct: 209 KQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITI 268

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNY+THH+KVMLLGHSD+Y QDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 269 SNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 328

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+D  SKEVTK+EDAPE EW+NW+WRSEGDL+LNGAF
Sbjct: 329 WEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAF 388

Query: 368 FTASGAG--ASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FT SGAG   SSSY++ASSL ARPSS VG IT  +GAL+C++GS C
Sbjct: 389 FTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Lsi02G004560 vs. TAIR10
Match: AT1G67750.1 (AT1G67750.1 Pectate lyase family protein)

HSP 1 Score: 681.4 bits (1757), Expect = 3.4e-196
Identity = 333/403 (82.63%), Postives = 357/403 (88.59%), Query Frame = 1

Query: 11  LFLFLFSVSLSAALTAAPAAVP--NPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDC 70
           L L LFS  L        A+ P   PELVVQEV+  I NA+ RRNLG LSCGTGNPIDDC
Sbjct: 8   LSLSLFSCLLLVLSPTFIASTPVSEPELVVQEVNEKI-NAS-RRNLGVLSCGTGNPIDDC 67

Query: 71  WRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDE 130
           WRCDP WEKNRQRLADC IGFGK+AIGGRDG+IYVVTD+ D D VNPKPGTLR+AVIQDE
Sbjct: 68  WRCDPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDE 127

Query: 131 PLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCK 190
           PLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGG CIT+QYVTNIIIHG+NIHDCK
Sbjct: 128 PLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCK 187

Query: 191 PGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITIS 250
             GN  VRDSP H+G+RT SDGD VSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAITIS
Sbjct: 188 RKGNAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITIS 247

Query: 251 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 310
           NNY++HH+KVMLLGHSDSYT+DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNYLSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 311 EMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFF 370
           +MYAIGGSAAPTINSQGNRF+APND   KEVTKYEDAP S+WK W+WRSEGDL LNGAFF
Sbjct: 308 QMYAIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFF 367

Query: 371 TASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           T SG GASSSYA+ASSL ARPSSLV ++T+NAGAL CRKGSRC
Sbjct: 368 TPSGGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

BLAST of Lsi02G004560 vs. TAIR10
Match: AT3G27400.1 (AT3G27400.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 653.3 bits (1684), Expect = 1.0e-187
Identity = 307/401 (76.56%), Postives = 350/401 (87.28%), Query Frame = 1

Query: 12  FLFLFSVSLSAALTAA-PAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDCWR 71
           FL L ++  + A +++ P  V +P LVV +V+RS+ NA+ RR+L YLSC TGNPIDDCWR
Sbjct: 13  FLLLLTIGNTLAFSSSLPDHVQDPNLVVDDVNRSVFNAS-RRSLAYLSCRTGNPIDDCWR 72

Query: 72  CDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEPL 131
           CDPNWE NRQRLADC IGFGKNAIGGR G+IYVVTD  +DDPVNP+PGTLRYAV Q+EPL
Sbjct: 73  CDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEEPL 132

Query: 132 WIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPG 191
           WIIF RDMVIRLK+ELI+ SFKTIDGRG+SVHI  GPC+ I Y TNIIIHG+NIHDCKPG
Sbjct: 133 WIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCKPG 192

Query: 192 GNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNN 251
               ++D P H G+   SDGD V+IFGG HVW+DHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 193 SGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITISNN 252

Query: 252 YMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 311
           +MTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 253 HMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 312

Query: 312 YAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTA 371
           YAIGGSA+PTI SQGNRF+APN RF+KEVTK+EDAPES+W++W+WRSEGD++LNGA+F  
Sbjct: 313 YAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYFRE 372

Query: 372 SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           SGA A S+YARASSL ARPSSLVG+ITT AG L+CR+G RC
Sbjct: 373 SGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Lsi02G004560 vs. TAIR10
Match: AT3G07010.1 (AT3G07010.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 622.1 bits (1603), Expect = 2.5e-178
Identity = 287/382 (75.13%), Postives = 330/382 (86.39%), Query Frame = 1

Query: 30  AVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDDCWRCDPNWEKNRQRLADCGIGF 89
           AV NP+ V   V  SI N+T+RR LGY SC TGNPIDDCWRCD  W+  R+RLADC IGF
Sbjct: 35  AVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCSIGF 94

Query: 90  GKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQDEPLWIIFARDMVIRLKEELIMN 149
           G+NAIGGRDG+ YVVTD GDDDPVNP PGTLR+AVIQDEPLWIIF RDMVI LK+ELIMN
Sbjct: 95  GRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQELIMN 154

Query: 150 SFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDCKPGGNTDVRDSPRHFGFRTISD 209
           SFKTIDGRG +VHIA G C+TIQYVTNII+HG+++HDCKP GN  VR SP H+GFR+++D
Sbjct: 155 SFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRSMAD 214

Query: 210 GDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQ 269
           GD +SIFG SH+W+DH SLSNC DGL+DA+  STAIT+SNN+ THH++VMLLGHSDSYT+
Sbjct: 215 GDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDSYTR 274

Query: 270 DKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAAPTINSQGNRFV 329
           DK MQVTIA+NHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRF+
Sbjct: 275 DKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGNRFL 334

Query: 330 APNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAFFTASGAGASSSYARASSLGARP 389
           AP + F+KEVTK E   ES+WK+W+WRSEGDL LNGAFFT SGAGA ++YARASSL A+ 
Sbjct: 335 APVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLSAKS 394

Query: 390 SSLVGTITTNAGALNCRKGSRC 412
           SSLVGT+T+ +GALNCR G RC
Sbjct: 395 SSLVGTMTSYSGALNCRAGRRC 416

BLAST of Lsi02G004560 vs. NCBI nr
Match: gi|659067371|ref|XP_008439105.1| (PREDICTED: probable pectate lyase 22 [Cucumis melo])

HSP 1 Score: 836.6 bits (2160), Expect = 1.8e-239
Identity = 401/411 (97.57%), Postives = 408/411 (99.27%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MAPPTVPLSLL LFLFS+SLSAAL++APAAV +PELVVQEVHRSI+NATKRRNLGYLSCG
Sbjct: 1   MAPPTVPLSLLSLFLFSISLSAALSSAPAAVSDPELVVQEVHRSIINATKRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTDAGDDDPVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDAGDDDPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFFT+SGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 LMLNGAFFTSSGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 411

BLAST of Lsi02G004560 vs. NCBI nr
Match: gi|449469590|ref|XP_004152502.1| (PREDICTED: probable pectate lyase 5 [Cucumis sativus])

HSP 1 Score: 826.2 bits (2133), Expect = 2.5e-236
Identity = 398/413 (96.37%), Postives = 405/413 (98.06%), Query Frame = 1

Query: 1   MAPPTVPLSL--LFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLS 60
           MAPP +PLSL  LFLFLFS+SLSAAL+  PAAV NPELVVQEVHRSI+NATKRRNLGYLS
Sbjct: 1   MAPPALPLSLLSLFLFLFSISLSAALSPNPAAVSNPELVVQEVHRSIINATKRRNLGYLS 60

Query: 61  CGTGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPG 120
           CGTGNPIDDCWRCD NWEKNRQRLADCGIGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPG
Sbjct: 61  CGTGNPIDDCWRCDSNWEKNRQRLADCGIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPG 120

Query: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180
           TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII
Sbjct: 121 TLRYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNII 180

Query: 181 IHGLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240
           IHGLNIHDCK GGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA
Sbjct: 181 IHGLNIHDCKQGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDA 240

Query: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300
           IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF
Sbjct: 241 IHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYF 300

Query: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360
           HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE
Sbjct: 301 HVVNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSE 360

Query: 361 GDLMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           GDLMLNGA+FTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC
Sbjct: 361 GDLMLNGAYFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 413

BLAST of Lsi02G004560 vs. NCBI nr
Match: gi|694358282|ref|XP_009359518.1| (PREDICTED: probable pectate lyase 18 [Pyrus x bretschneideri])

HSP 1 Score: 744.6 bits (1921), Expect = 9.4e-212
Identity = 358/411 (87.10%), Postives = 381/411 (92.70%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MA P++  SLL L LFS+   + +++ P  + +PELVVQEV R+I ++  RRNLGYLSCG
Sbjct: 1   MARPSLGPSLLSLLLFSLLSPSLISSRPLHLQDPELVVQEVQRNISDSVSRRNLGYLSCG 60

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCDPNWEKNRQRLADC IGFGKNAIGGRDG+IYVVTD+GDD+PVNPKPGTL
Sbjct: 61  TGNPIDDCWRCDPNWEKNRQRLADCAIGFGKNAIGGRDGKIYVVTDSGDDNPVNPKPGTL 120

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           R+AVIQDEPLWIIF RDM I+LKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH
Sbjct: 121 RHAVIQDEPLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGN  VRDSP HFG+RTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GLNIHDCKQGGNAMVRDSPGHFGWRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSA PTINSQGNRFVAP+ RFSKEVTK+EDAPE EWKNW+WRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSANPTINSQGNRFVAPDIRFSKEVTKHEDAPEGEWKNWNWRSEGD 360

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LMLNGAFF ASGAGASSSYARASSLGARPSSLVG+ITT AGAL+CRKGS C
Sbjct: 361 LMLNGAFFRASGAGASSSYARASSLGARPSSLVGSITTAAGALSCRKGSHC 411

BLAST of Lsi02G004560 vs. NCBI nr
Match: gi|703123867|ref|XP_010102919.1| (hypothetical protein L484_018936 [Morus notabilis])

HSP 1 Score: 741.1 bits (1912), Expect = 1.0e-210
Identity = 354/404 (87.62%), Postives = 376/404 (93.07%), Query Frame = 1

Query: 8   LSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCGTGNPIDD 67
           L  L +FLFS  +    T   +  P+PE VV EVHRSI  +  RRNLGYLSCGTGNPIDD
Sbjct: 10  LLTLIVFLFSFQIP---TLHSSPTPDPERVVGEVHRSINASVSRRNLGYLSCGTGNPIDD 69

Query: 68  CWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTLRYAVIQD 127
           CWRCDPNWEKNRQRLADC IGFGKNA+GGRDG+IYVVTD+ D+D VNPKPGTLRYAVIQD
Sbjct: 70  CWRCDPNWEKNRQRLADCAIGFGKNAVGGRDGKIYVVTDSSDNDVVNPKPGTLRYAVIQD 129

Query: 128 EPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIHGLNIHDC 187
           EPLWIIFARDMVI+LKEELIMNSFKTIDGRGASVHIAGGPCITIQ+VTNIIIHGLNIHDC
Sbjct: 130 EPLWIIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGPCITIQFVTNIIIHGLNIHDC 189

Query: 188 KPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIHGSTAITI 247
           KPGGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDHCSLSNC DGLIDAIHGSTAIT+
Sbjct: 190 KPGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHCSLSNCQDGLIDAIHGSTAITL 249

Query: 248 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 307
           SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH
Sbjct: 250 SNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTH 309

Query: 308 WEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGDLMLNGAF 367
           WEMYAIGGSA PTINSQGNRF+AP+ RFSKEVTK+EDAPESEWKNW+WRSEGDLM+NGAF
Sbjct: 310 WEMYAIGGSANPTINSQGNRFLAPDKRFSKEVTKHEDAPESEWKNWNWRSEGDLMVNGAF 369

Query: 368 FTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           FTASGAGASSSYA+ASSLGARPS+LVGTITTNAGAL+CRKGSRC
Sbjct: 370 FTASGAGASSSYAKASSLGARPSTLVGTITTNAGALSCRKGSRC 410

BLAST of Lsi02G004560 vs. NCBI nr
Match: gi|284504570|gb|ADB90475.1| (pectate lyase [Gossypium herbaceum])

HSP 1 Score: 739.2 bits (1907), Expect = 3.9e-210
Identity = 359/411 (87.35%), Postives = 385/411 (93.67%), Query Frame = 1

Query: 1   MAPPTVPLSLLFLFLFSVSLSAALTAAPAAVPNPELVVQEVHRSIVNATKRRNLGYLSCG 60
           MA P++PL  LFLF F + L   ++++P  V +PELVVQ+VHR+I NA+ RRNLGYLSCG
Sbjct: 7   MAIPSLPL--LFLFTFLLLLPLLISSSP--VQDPELVVQDVHRAI-NAS-RRNLGYLSCG 66

Query: 61  TGNPIDDCWRCDPNWEKNRQRLADCGIGFGKNAIGGRDGQIYVVTDAGDDDPVNPKPGTL 120
           TGNPIDDCWRCDPNWE NRQ+LADC IGFGKNAIGGRDG+IYVVTD+GDDDPVNPKPGTL
Sbjct: 67  TGNPIDDCWRCDPNWETNRQKLADCAIGFGKNAIGGRDGKIYVVTDSGDDDPVNPKPGTL 126

Query: 121 RYAVIQDEPLWIIFARDMVIRLKEELIMNSFKTIDGRGASVHIAGGPCITIQYVTNIIIH 180
           R+AVIQDEPLWIIFARDM I+LKEELIMNSFKTIDGRGASVHIAGGPCIT+QYVTNIIIH
Sbjct: 127 RHAVIQDEPLWIIFARDMTIQLKEELIMNSFKTIDGRGASVHIAGGPCITVQYVTNIIIH 186

Query: 181 GLNIHDCKPGGNTDVRDSPRHFGFRTISDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIH 240
           GLNIHDCK GGN  VRDSPRH+G+RTISDGDGVSIFGGSHVWVDH SLSNC DGLIDAIH
Sbjct: 187 GLNIHDCKQGGNAMVRDSPRHYGWRTISDGDGVSIFGGSHVWVDHNSLSNCKDGLIDAIH 246

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 247 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 306

Query: 301 VNNDYTHWEMYAIGGSAAPTINSQGNRFVAPNDRFSKEVTKYEDAPESEWKNWHWRSEGD 360
           VNNDYTHWEMYAIGGSA PTINSQGNRF APN+RFSKEVTK+EDAPESEWK+W+WRSEGD
Sbjct: 307 VNNDYTHWEMYAIGGSANPTINSQGNRFTAPNNRFSKEVTKHEDAPESEWKSWNWRSEGD 366

Query: 361 LMLNGAFFTASGAGASSSYARASSLGARPSSLVGTITTNAGALNCRKGSRC 412
           LM+NGAFF ASGAGASSSYA+ASSLGARPSSLV TITTNAGALNC+KGSRC
Sbjct: 367 LMVNGAFFIASGAGASSSYAKASSLGARPSSLVATITTNAGALNCKKGSRC 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLY18_ARATH2.2e-19781.59Probable pectate lyase 18 OS=Arabidopsis thaliana GN=At4g24780 PE=2 SV=2[more]
PLY22_ARATH8.5e-19780.30Probable pectate lyase 22 OS=Arabidopsis thaliana GN=At5g63180 PE=2 SV=1[more]
PLY5_ARATH6.1e-19582.63Probable pectate lyase 5 OS=Arabidopsis thaliana GN=At1g67750 PE=2 SV=2[more]
PLY11_ARATH1.8e-18676.56Putative pectate lyase 11 OS=Arabidopsis thaliana GN=At3g27400 PE=3 SV=2[more]
PLY_ZINVI1.3e-18476.92Pectate lyase OS=Zinnia violacea PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LU82_CUCSA1.7e-23696.37Pectate lyase OS=Cucumis sativus GN=Csa_1G049960 PE=3 SV=1[more]
W9RSL5_9ROSA7.2e-21187.62Pectate lyase OS=Morus notabilis GN=L484_018936 PE=3 SV=1[more]
D3IZZ1_GOSHE2.7e-21087.35Pectate lyase OS=Gossypium herbaceum GN=Pel PE=3 SV=1[more]
I1T4H1_GOSAI4.7e-21087.35Pectate lyase OS=Gossypium aridum PE=3 SV=1[more]
I1T4F2_9ROSI4.7e-21087.35Pectate lyase OS=Gossypium laxum PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G24780.11.3e-19881.59 Pectin lyase-like superfamily protein[more]
AT5G63180.14.8e-19880.30 Pectin lyase-like superfamily protein[more]
AT1G67750.13.4e-19682.63 Pectate lyase family protein[more]
AT3G27400.11.0e-18776.56 Pectin lyase-like superfamily protein[more]
AT3G07010.12.5e-17875.13 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659067371|ref|XP_008439105.1|1.8e-23997.57PREDICTED: probable pectate lyase 22 [Cucumis melo][more]
gi|449469590|ref|XP_004152502.1|2.5e-23696.37PREDICTED: probable pectate lyase 5 [Cucumis sativus][more]
gi|694358282|ref|XP_009359518.1|9.4e-21287.10PREDICTED: probable pectate lyase 18 [Pyrus x bretschneideri][more]
gi|703123867|ref|XP_010102919.1|1.0e-21087.62hypothetical protein L484_018936 [Morus notabilis][more]
gi|284504570|gb|ADB90475.1|3.9e-21087.35pectate lyase [Gossypium herbaceum][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018082AmbAllergen
IPR012334Pectin_lyas_fold
IPR011050Pectin_lyase_fold/virulence
IPR002022Pec_lyase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi02G004560.1Lsi02G004560.1mRNA


Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002022Pectate lyase/Amb allergenPFAMPF00544Pec_lyase_Ccoord: 147..328
score: 4.7
IPR002022Pectate lyase/Amb allergenSMARTSM00656amb_allcoord: 137..334
score: 8.7E
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 38..406
score: 7.39E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 63..403
score: 3.0E
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 85..102
score: 3.1E-85coord: 382..405
score: 3.1E-85coord: 289..308
score: 3.1E-85coord: 353..377
score: 3.1E-85coord: 209..230
score: 3.1E-85coord: 311..330
score: 3.1E-85coord: 109..134
score: 3.1E-85coord: 145..161
score: 3.1
NoneNo IPR availablePANTHERPTHR31683FAMILY NOT NAMEDcoord: 8..411
score:
NoneNo IPR availablePANTHERPTHR31683:SF28PECTATE LYASE 22-RELATEDcoord: 8..411
score: