Lsi04G019650 (gene) Bottle gourd (USVL1VR-Ls) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Lsi04G019650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls))
Description	Glucan endo-1,3-beta-glucosidase
Location	chr04 : 26722912 .. 26723545 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCATTCCATCTCCCTCCTATAAATTCCTCCTTCATCTTCTTCCTTTTCAGATCTCTTCCACTTCCTTCAATAATTCCCTCTGATCGCAGCACAACAGAGTACTATCAATGGCAAAAGCGGCCACTCTCTCCTTTTTGCTTCTATTCCTTTCGTTCCTTTCAGGTAAAATTTCTCCACATCTTTTCAAATTCCGCCAAGTTTTCAAATAATTACTGAACAATTTGATCTTCTTCCGATCGATTTCTTCTTCAGGACGAGATTTGCTGGCAGTAAATGCACAGGTGAAATCATCGTTATTATGCAATTAGGGTTTACACAAACAATCATAATAATTCTCTGAAAACTGAATTCGAATTCTTCTTTTTCTTTTTTGTGCGATGAATAATTTGCAGAAAACGTGGTGCATTCCGAAGCCTTCGTCGGATCAAGCGACGTTGTTGGCGAATACCAATTACGCTTGTGCTCAGGTAGATTGCAAAATAATGCAACAAGGAAACCCCTGCTTTTATCCTGATAATTTGTTCAACCATGCTGCCATAGCTATGAGTTTGTATTACAATTCCAAAGGGAAGAATCAATGGAACTGTGACTTTAGAGGCTCTGGTCTCGTAGTCACCACTGATCCAAGTTAG

mRNA sequence

ATTCATTCCATCTCCCTCCTATAAATTCCTCCTTCATCTTCTTCCTTTTCAGATCTCTTCCACTTCCTTCAATAATTCCCTCTGATCGCAGCACAACAGAGTACTATCAATGGCAAAAGCGGCCACTCTCTCCTTTTTGCTTCTATTCCTTTCGTTCCTTTCAGGACGAGATTTGCTGGCAGTAAATGCACAGAAAACGTGGTGCATTCCGAAGCCTTCGTCGGATCAAGCGACGTTGTTGGCGAATACCAATTACGCTTGTGCTCAGGTAGATTGCAAAATAATGCAACAAGGAAACCCCTGCTTTTATCCTGATAATTTGTTCAACCATGCTGCCATAGCTATGAGTTTGTATTACAATTCCAAAGGGAAGAATCAATGGAACTGTGACTTTAGAGGCTCTGGTCTCGTAGTCACCACTGATCCAAGTTAG

Coding sequence (CDS)

ATGGCAAAAGCGGCCACTCTCTCCTTTTTGCTTCTATTCCTTTCGTTCCTTTCAGGACGAGATTTGCTGGCAGTAAATGCACAGAAAACGTGGTGCATTCCGAAGCCTTCGTCGGATCAAGCGACGTTGTTGGCGAATACCAATTACGCTTGTGCTCAGGTAGATTGCAAAATAATGCAACAAGGAAACCCCTGCTTTTATCCTGATAATTTGTTCAACCATGCTGCCATAGCTATGAGTTTGTATTACAATTCCAAAGGGAAGAATCAATGGAACTGTGACTTTAGAGGCTCTGGTCTCGTAGTCACCACTGATCCAAGTTAG

Protein sequence

MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS

Homology

[Close]

BLAST of Lsi04G019650 vs. Swiss-Prot
Match: ALL9_OLEEU (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea GN=OLE9 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 1.6e-17
Identity = 38/82 (46.34%), Postives = 49/82 (59.76%), Query Frame = 1


Query: 27  AQKTWCIPKPSSDQATLLANTNYACAQ-VDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNS 86
           A  +WC+PKP      L  N NYAC Q +DC  +Q G  CF P+ +  HAA  M+LYY S
Sbjct: 368 AAGSWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQS 427

Query: 87  KGKNQWNCDFRGSGLVVTTDPS 108
            G+N WNCDF  +  +  T+PS
Sbjct: 428 AGRNSWNCDFSQTATLTNTNPS 449

[Close]

BLAST of Lsi04G019650 vs. Swiss-Prot
Match: E13B_WHEAT (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum GN=GLC1 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 8.6e-16
Identity = 34/77 (44.16%), Postives = 48/77 (62.34%), Query Frame = 1


Query: 31  WCIPKPSSDQATLLANTNYACAQVDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQ 90
           WC+ K  ++   L  N NYAC  VDCK +Q G  CF P++L  HA+  M+ YY + G   
Sbjct: 377 WCVAKDGANGTDLQNNINYACGFVDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTD 436

Query: 91  WNCDFRGSGLVVTTDPS 108
             CDF+G+G+V ++DPS
Sbjct: 437 LACDFKGTGIVTSSDPS 453

[Close]

BLAST of Lsi04G019650 vs. Swiss-Prot
Match: ALL10_OLEEU (Major pollen allergen Ole e 10 OS=Olea europaea PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-15
Identity = 34/81 (41.98%), Postives = 53/81 (65.43%), Query Frame = 1


Query: 29  KTWCIPKPSSDQATLLANTNYACAQ--VDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNSK 88
           K WC+PK  +  A L +N +Y C+Q  +DC  +Q    CF P+ +  HA+ AM+ +Y SK
Sbjct: 35  KKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSK 94

Query: 89  GKNQWNCDFRGSGLVVTTDPS 108
           G+N ++CDF G+G + ++DPS
Sbjct: 95  GRNDFDCDFSGTGAITSSDPS 115

[Close]

BLAST of Lsi04G019650 vs. Swiss-Prot
Match: E131_ARATH (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana GN=At1g11820 PE=1 SV=3)

HSP 1 Score: 72.0 bits (175), Expect = 4.4e-12
Identity = 34/100 (34.00%), Postives = 55/100 (55.00%), Query Frame = 1


Query: 12  LFLSFLSGRDLLAVN--AQKTWCIPKPSSDQATLLANTNYACA--QVDCKIMQQGNPCFY 71
           ++L  +SG      N    +T+CI     D  TL A  ++AC   + +C  +Q G  C+ 
Sbjct: 360 VYLLHVSGSGTFLANDTTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQ 419

Query: 72  PDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           P+N+  HA+ A + YY  +G+   +CDF+G  ++ TTDPS
Sbjct: 420 PNNVKGHASFAFNSYYQKEGRASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of Lsi04G019650 vs. Swiss-Prot
Match: E137_ARATH (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 5.8e-12
Identity = 29/78 (37.18%), Postives = 46/78 (58.97%), Query Frame = 1


Query: 31  WCIPKPSSDQATLLANTNYACAQ-VDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNSKGKN 90
           WC+PK  +    L A+ ++AC   +DC  +Q G  CF P+N+ +HAA AM++Y+    K 
Sbjct: 364 WCVPKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQ 423

Query: 91  QWNCDFRGSGLVVTTDPS 108
             +CDF  +  V + +PS
Sbjct: 424 PTDCDFSKTATVTSQNPS 441

[Close]

BLAST of Lsi04G019650 vs. TrEMBL
Match: A0A0A0L4I1_CUCSA (Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus GN=Csa_4G622750 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.1e-46
Identity = 94/108 (87.04%), Postives = 100/108 (92.59%), Query Frame = 1


Query: 1   MAK-AATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAK AA+LSFLLLFLSFLSGRDLLA+NAQKTWCIPKPSSDQATLLAN NYACAQVDC+IM
Sbjct: 1   MAKPAASLSFLLLFLSFLSGRDLLALNAQKTWCIPKPSSDQATLLANINYACAQVDCRIM 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q+G PC YPD L N AAIAMSLYY+SKGKNQWNCDFRGSGL+V TDPS
Sbjct: 61  QKGCPCSYPDTLINRAAIAMSLYYHSKGKNQWNCDFRGSGLMVITDPS 108

[Close]

BLAST of Lsi04G019650 vs. TrEMBL
Match: V4T5M7_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022921mg PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 5.0e-39
Identity = 74/107 (69.16%), Postives = 88/107 (82.24%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQ 60
           MA  A L   LL LSF SG +LL VN QKTWC+ KPSSDQA LLAN NYAC+Q+DC+I+Q
Sbjct: 1   MAPKAALYLFLLLLSFTSGGNLLVVNGQKTWCVAKPSSDQAALLANINYACSQIDCRILQ 60

Query: 61  QGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +G PCFYPDNL NHA+I+M+LYY +KG+N+WNCDFR S L+VTTDPS
Sbjct: 61  KGYPCFYPDNLMNHASISMNLYYQAKGRNRWNCDFRNSALIVTTDPS 107

[Close]

BLAST of Lsi04G019650 vs. TrEMBL
Match: A0A061FGN1_THECC (Carbohydrate-binding X8 domain superfamily protein OS=Theobroma cacao GN=TCM_032235 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 5.0e-39
Identity = 75/107 (70.09%), Postives = 89/107 (83.18%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQ 60
           MAK A  S LLL +SF SG  L+ VN QK+WC+ KPSSDQATLLAN NYAC+QVDC+++Q
Sbjct: 1   MAKVAAFSLLLLLVSFTSGGTLMMVNGQKSWCVAKPSSDQATLLANINYACSQVDCRVLQ 60

Query: 61  QGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +G PCF PDNL NHA+IAM+LYY +KG+N+WNCDFR SGLVV TDPS
Sbjct: 61  RGCPCFTPDNLMNHASIAMNLYYQAKGRNRWNCDFRSSGLVVITDPS 107

[Close]

BLAST of Lsi04G019650 vs. TrEMBL
Match: A0A059BP19_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01381 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 9.5e-38
Identity = 76/108 (70.37%), Postives = 89/108 (82.41%), Query Frame = 1


Query: 1   MAKAA-TLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAK A +L FLLL L+F SG  +  VN QKTWC+ KPSSDQATLLAN NY+C+QVDCKI+
Sbjct: 1   MAKVAFSLIFLLLSLAFGSGAPVTVVNGQKTWCVAKPSSDQATLLANINYSCSQVDCKIL 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q+G PCFYPD+L NHA++AM+LYY S GKN WNCDFR SGLVV +DPS
Sbjct: 61  QKGGPCFYPDDLTNHASVAMNLYYQSHGKNHWNCDFRNSGLVVVSDPS 108

[Close]

BLAST of Lsi04G019650 vs. TrEMBL
Match: A0A0D2QXA8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_002G044300 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.6e-37
Identity = 75/108 (69.44%), Postives = 90/108 (83.33%), Query Frame = 1


Query: 1   MAK-AATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAK AA LS  LL  SF+ G  L+ VN QK+WC+ KPSSDQATLLAN N+AC+QVDC++M
Sbjct: 1   MAKVAAALSLSLLIFSFIPGGTLMMVNGQKSWCVAKPSSDQATLLANINFACSQVDCRVM 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q+G PCF PDNL NHA+IAM+LYY +KG+N+WNCDFRGSGLVV T+PS
Sbjct: 61  QKGCPCFSPDNLMNHASIAMNLYYQAKGRNKWNCDFRGSGLVVITNPS 108

[Close]

BLAST of Lsi04G019650 vs. TAIR10
Match: AT1G78520.1 (AT1G78520.1 Carbohydrate-binding X8 domain superfamily protein)

HSP 1 Score: 128.6 bits (322), Expect = 2.2e-30
Identity = 57/108 (52.78%), Postives = 77/108 (71.30%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQ-KTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAKA      L+FL  +S R+ + VNA+ KTWC+ KPSSDQ  L  N N+AC+ VDC+++
Sbjct: 1   MAKAWICLSFLIFLYLVSERNFINVNAETKTWCVAKPSSDQVALQDNINFACSHVDCRVL 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
             G PC+ P NL NHA+IAM+LYY + G+N WNC+F+ SGL+  T+PS
Sbjct: 61  LSGCPCYSPSNLINHASIAMNLYYQANGRNYWNCNFKNSGLITITNPS 108

[Close]

BLAST of Lsi04G019650 vs. TAIR10
Match: AT2G43670.1 (AT2G43670.1 Carbohydrate-binding X8 domain superfamily protein)

HSP 1 Score: 104.4 bits (259), Expect = 4.5e-23
Identity = 45/111 (40.54%), Postives = 73/111 (65.77%), Query Frame = 1


Query: 1   MAKAAT-LSFLLLFLSFLSGRDLLAVNAQKT---WCIPKPSSDQATLLANTNYACAQVDC 60
           MAKA   L F++    +  G  +    A ++   WC+ KPS+D   L  N N+AC+++DC
Sbjct: 1   MAKAQICLCFIIFLYLWPEGNFIKVTKADRSAGDWCVAKPSTDNERLQENINFACSKIDC 60

Query: 61  KIMQQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +I+ +G  C+ PD++ + A++AM+LYY ++G++ WNC+F GSGL+  TDPS
Sbjct: 61  QIISEGGACYLPDSIISRASVAMNLYYQAQGRHFWNCNFEGSGLIGITDPS 111

[Close]

BLAST of Lsi04G019650 vs. TAIR10
Match: AT3G28250.1 (AT3G28250.1 Carbohydrate-binding X8 domain superfamily protein)

HSP 1 Score: 97.4 bits (241), Expect = 5.5e-21
Identity = 38/77 (49.35%), Postives = 54/77 (70.13%), Query Frame = 1


Query: 31  WCIPKPSSDQATLLANTNYACAQVDCKIMQQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQ 90
           WC+ KP +    L+ N NYAC+ VDC+I+     C+ PDN++N A++ M+LYY ++G+N 
Sbjct: 3   WCVAKPGTLTEQLINNLNYACSIVDCQIISTRGACYSPDNIYNMASVVMNLYYQAEGRNF 62

Query: 91  WNCDFRGSGLVVTTDPS 108
           WNC+F  SGLV  TDPS
Sbjct: 63  WNCNFGDSGLVAITDPS 79

[Close]

BLAST of Lsi04G019650 vs. TAIR10
Match: AT5G53600.1 (AT5G53600.1 Carbohydrate-binding X8 domain superfamily protein)

HSP 1 Score: 87.0 bits (214), Expect = 7.5e-18
Identity = 41/101 (40.59%), Postives = 58/101 (57.43%), Query Frame = 1


Query: 10  LLLFLSFLSGRDLL---AVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQQGNPCF 69
           LL FL  LS   +     V  ++TWC   P+S    L +N N+AC  VDC  +Q G  C+
Sbjct: 5   LLTFLFLLSAAVIYHIPVVTCRRTWCTAMPTSTTEQLQSNINFACNHVDCAPIQPGGFCY 64

Query: 70  YPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           YP+ L +HAA AM+ YY S+G     C F  +G ++++DPS
Sbjct: 65  YPNTLLDHAAFAMTRYYRSQGHTYAACSFGNTGYIISSDPS 105

[Close]

BLAST of Lsi04G019650 vs. TAIR10
Match: AT4G09462.1 (AT4G09462.1 Carbohydrate-binding X8 domain superfamily protein)

HSP 1 Score: 85.5 bits (210), Expect = 2.2e-17
Identity = 46/108 (42.59%), Postives = 61/108 (56.48%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQ-VDCKIM 60
           MAK +  S L L    LS   +  V + KTWCI    +  A L AN N+AC+Q VDC+ +
Sbjct: 1   MAKIS--SPLALLFIMLSSIMINHVASSKTWCIATLIATNAQLQANINFACSQGVDCRPI 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q G  CF P+NL NHA+  M+ YY + G+    C F+ +G    TDPS
Sbjct: 61  QPGGSCFIPNNLANHASFVMNSYYQTHGRTNQACSFKNTGTFAATDPS 106

[Close]

BLAST of Lsi04G019650 vs. NCBI nr
Match: gi|659103025|ref|XP_008452435.1| (PREDICTED: glucan endo-1,3-beta-D-glucosidase [Cucumis melo])

HSP 1 Score: 194.5 bits (493), Expect = 9.4e-47
Identity = 94/108 (87.04%), Postives = 100/108 (92.59%), Query Frame = 1


Query: 1   MAK-AATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAK AA+LSFLLLFLSFLSGRDLLA+NAQKTWCIPKPSSDQATLLAN NYACAQVDC+IM
Sbjct: 1   MAKPAASLSFLLLFLSFLSGRDLLALNAQKTWCIPKPSSDQATLLANINYACAQVDCRIM 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q+G PC YPD L N AAIAMSLYY+SKGKNQWNCDFRGSGLV+ TDPS
Sbjct: 61  QKGCPCSYPDTLINRAAIAMSLYYHSKGKNQWNCDFRGSGLVIITDPS 108

[Close]

BLAST of Lsi04G019650 vs. NCBI nr
Match: gi|449456541|ref|XP_004146007.1| (PREDICTED: glucan endo-1,3-beta-D-glucosidase [Cucumis sativus])

HSP 1 Score: 193.7 bits (491), Expect = 1.6e-46
Identity = 94/108 (87.04%), Postives = 100/108 (92.59%), Query Frame = 1


Query: 1   MAK-AATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIM 60
           MAK AA+LSFLLLFLSFLSGRDLLA+NAQKTWCIPKPSSDQATLLAN NYACAQVDC+IM
Sbjct: 1   MAKPAASLSFLLLFLSFLSGRDLLALNAQKTWCIPKPSSDQATLLANINYACAQVDCRIM 60

Query: 61  QQGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           Q+G PC YPD L N AAIAMSLYY+SKGKNQWNCDFRGSGL+V TDPS
Sbjct: 61  QKGCPCSYPDTLINRAAIAMSLYYHSKGKNQWNCDFRGSGLMVITDPS 108

[Close]

BLAST of Lsi04G019650 vs. NCBI nr
Match: gi|567899660|ref|XP_006442318.1| (hypothetical protein CICLE_v10022921mg [Citrus clementina])

HSP 1 Score: 168.3 bits (425), Expect = 7.2e-39
Identity = 74/107 (69.16%), Postives = 88/107 (82.24%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQ 60
           MA  A L   LL LSF SG +LL VN QKTWC+ KPSSDQA LLAN NYAC+Q+DC+I+Q
Sbjct: 1   MAPKAALYLFLLLLSFTSGGNLLVVNGQKTWCVAKPSSDQAALLANINYACSQIDCRILQ 60

Query: 61  QGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +G PCFYPDNL NHA+I+M+LYY +KG+N+WNCDFR S L+VTTDPS
Sbjct: 61  KGYPCFYPDNLMNHASISMNLYYQAKGRNRWNCDFRNSALIVTTDPS 107

[Close]

BLAST of Lsi04G019650 vs. NCBI nr
Match: gi|590611391|ref|XP_007022089.1| (Carbohydrate-binding X8 domain superfamily protein [Theobroma cacao])

HSP 1 Score: 168.3 bits (425), Expect = 7.2e-39
Identity = 75/107 (70.09%), Postives = 89/107 (83.18%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQ 60
           MAK A  S LLL +SF SG  L+ VN QK+WC+ KPSSDQATLLAN NYAC+QVDC+++Q
Sbjct: 1   MAKVAAFSLLLLLVSFTSGGTLMMVNGQKSWCVAKPSSDQATLLANINYACSQVDCRVLQ 60

Query: 61  QGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +G PCF PDNL NHA+IAM+LYY +KG+N+WNCDFR SGLVV TDPS
Sbjct: 61  RGCPCFTPDNLMNHASIAMNLYYQAKGRNRWNCDFRSSGLVVITDPS 107

[Close]

BLAST of Lsi04G019650 vs. NCBI nr
Match: gi|568847963|ref|XP_006477797.1| (PREDICTED: glucan endo-1,3-beta-D-glucosidase [Citrus sinensis])

HSP 1 Score: 167.9 bits (424), Expect = 9.4e-39
Identity = 74/107 (69.16%), Postives = 88/107 (82.24%), Query Frame = 1


Query: 1   MAKAATLSFLLLFLSFLSGRDLLAVNAQKTWCIPKPSSDQATLLANTNYACAQVDCKIMQ 60
           MA  A L   LL LSF SG +LL VN QKTWC+ KPSSDQA LLAN NYAC+Q+DC+I+Q
Sbjct: 1   MAPKAALYLFLLLLSFTSGGNLLMVNGQKTWCVAKPSSDQAALLANINYACSQIDCRILQ 60

Query: 61  QGNPCFYPDNLFNHAAIAMSLYYNSKGKNQWNCDFRGSGLVVTTDPS 108
           +G PCFYPDNL NHA+I+M+LYY +KG+N+WNCDFR S L+VTTDPS
Sbjct: 61  KGYPCFYPDNLMNHASISMNLYYQAKGRNRWNCDFRNSALIVTTDPS 107

The following BLAST results are available for this feature:

BLAST of Lsi04G019650 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ALL9_OLEEU	1.6e-17	46.34	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea GN=OLE9 PE=1 SV=1	[more]
E13B_WHEAT	8.6e-16	44.16	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum GN=GLC1 PE=2 SV=1	[more]
ALL10_OLEEU	1.1e-15	41.98	Major pollen allergen Ole e 10 OS=Olea europaea PE=1 SV=1	[more]
E131_ARATH	4.4e-12	34.00	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana GN=At1g11820 PE=1 SV=...	[more]
E137_ARATH	5.8e-12	37.18	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana GN=At4g34480 PE=1 SV=...	[more]

BLAST of Lsi04G019650 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4I1_CUCSA	1.1e-46	87.04	Hydrolase, hydrolyzing O-glycosyl compounds OS=Cucumis sativus GN=Csa_4G622750 P...	[more]
V4T5M7_9ROSI	5.0e-39	69.16	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10022921mg PE=4 SV=1	[more]
A0A061FGN1_THECC	5.0e-39	70.09	Carbohydrate-binding X8 domain superfamily protein OS=Theobroma cacao GN=TCM_032...	[more]
A0A059BP19_EUCGR	9.5e-38	70.37	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_F01381 PE=4 SV=1	[more]
A0A0D2QXA8_GOSRA	1.6e-37	69.44	Uncharacterized protein OS=Gossypium raimondii GN=B456_002G044300 PE=4 SV=1	[more]

BLAST of Lsi04G019650 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G78520.1	2.2e-30	52.78	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43670.1	4.5e-23	40.54	Carbohydrate-binding X8 domain superfamily protein	[more]
AT3G28250.1	5.5e-21	49.35	Carbohydrate-binding X8 domain superfamily protein	[more]
AT5G53600.1	7.5e-18	40.59	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G09462.1	2.2e-17	42.59	Carbohydrate-binding X8 domain superfamily protein	[more]

BLAST of Lsi04G019650 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659103025\|ref\|XP_008452435.1\|	9.4e-47	87.04	PREDICTED: glucan endo-1,3-beta-D-glucosidase [Cucumis melo]	[more]
gi\|449456541\|ref\|XP_004146007.1\|	1.6e-46	87.04	PREDICTED: glucan endo-1,3-beta-D-glucosidase [Cucumis sativus]	[more]
gi\|567899660\|ref\|XP_006442318.1\|	7.2e-39	69.16	hypothetical protein CICLE_v10022921mg [Citrus clementina]	[more]
gi\|590611391\|ref\|XP_007022089.1\|	7.2e-39	70.09	Carbohydrate-binding X8 domain superfamily protein [Theobroma cacao]	[more]
gi\|568847963\|ref\|XP_006477797.1\|	9.4e-39	69.16	PREDICTED: glucan endo-1,3-beta-D-glucosidase [Citrus sinensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR012946	X8

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018106	peptidyl-histidine phosphorylation
biological_process	GO:0008152	metabolic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0007165	signal transduction
biological_process	GO:0008150	biological_process
cellular_component	GO:0009365	protein histidine kinase complex
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004673	protein histidine kinase activity
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0004871	signal transducer activity
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G019650.1	Lsi04G019650.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Lagenaria siceraria
Date Performed: 2017-09-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 30..99 score: 4.7
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 30..107 score: 8.5
None	No IPR available	PANTHER	PTHR31044	FAMILY NOT NAMED	coord: 1..107 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Lsi04G019650	Csa4G622750	Cucumber (Chinese Long) v2	culsiB283
Lsi04G019650	CSPI04G22360	Wild cucumber (PI 183967)	cpilsiB283
Lsi04G019650	Cucsa.058100	Cucumber (Gy14) v1	cgylsiB047
Lsi04G019650	CmaCh09G006850	Cucurbita maxima (Rimu)	cmalsiB025
Lsi04G019650	CmaCh01G012780	Cucurbita maxima (Rimu)	cmalsiB432
Lsi04G019650	CmoCh01G013310	Cucurbita moschata (Rifu)	cmolsiB421
Lsi04G019650	CmoCh09G006690	Cucurbita moschata (Rifu)	cmolsiB013
Lsi04G019650	Cla023294	Watermelon (97103) v1	lsiwmB333
Lsi04G019650	MELO3C016683	Melon (DHL92) v3.5.1	lsimeB321
Lsi04G019650	ClCG11G017040	Watermelon (Charleston Gray)	lsiwcgB259
Lsi04G019650	MELO3C016635.2	Melon (DHL92) v3.6.1	lsimedB331
Lsi04G019650	MELO3C016683.2	Melon (DHL92) v3.6.1	lsimedB335
Lsi04G019650	Cla97C11G223080	Watermelon (97103) v2	lsiwmbB266
Lsi04G019650	Bhi09G002923	Wax gourd	lsiwgoB402
Lsi04G019650	Cp4.1LG06g05350	Cucurbita pepo (Zucchini)	cpelsiB639
Lsi04G019650	Cp4.1LG02g02240	Cucurbita pepo (Zucchini)	cpelsiB456
Lsi04G019650	CsGy4G020850	Cucumber (Gy14) v2	cgyblsiB263
Lsi04G019650	Carg14758	Silver-seed gourd	carlsiB286
Lsi04G019650	CsaV3_4G032700	Cucumber (Chinese Long) v3	cuclsiB304

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Lsi04G019650	Wild cucumber (PI 183967)	cpilsiB290
Lsi04G019650	Cucumber (Chinese Long) v2	culsiB290
Lsi04G019650	Silver-seed gourd	carlsiB577