Cucsa.301890 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.301890
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	L-lactate dehydrogenase
Location	scaffold02951 : 123600 .. 125959 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCTTTCTAACGCAATCCCCATTCAGAATCCGCCAAGTTTCTCTGCAACAAAAATCTTCATCGCCAACCACGATCTCCAAAAACCCATTTTCACAATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGTCAGTTTTGCCATTGATCATTGTTTTTTTtGGTTAATTTGGCGTTGTTTTACTGAAGAAATTTCCCTTTCGTTTGAATTGTTTTTGGTTGATTGGAATCAAATGAGTTGATCATTTTTGGGAAAAAGTAACCAAAATGAACCATAAAATTTCATATTTCTAATTTTGTTATATGAAGTTATTAGGCTCTTTCTAAAGTTCATAAATCAAATTAAAAGCTTTTAAGTGTAAGGAATTATCCATACAAACCAAATTATGTGTAGAGTAATTTATAACTTAACTCAAAAAAaTTAACTTTCACTTATAAATTTGGACTTATGTGATTTGGAGAAAGTTTGTTACTGTGTTTATAATTAAAACCCTAACAAATATGAGAGCTTTTTGTACTGATAACGGTTTCTTGTGTGGGGTGATTACAATAAGTACTCATTTATAACAACATTTTCAAAATGGTAAATACAACAAGTATGTTGTTAATCTATCAATATTTACAATATAGTAACTCCAACTGTTTTTGTTGTATTTACAATTTTTGAAAATTATTGCTATATAGGTTAATACTTTAAATTTAATTGTTATGTTTGTAATTGTTTGACCCACACCTGCAATAGTGTGAAGTTGGGATAGGGATTTGGCAATGTTAAATGGGAAAAGTGTAAAAAAaGATATCACATCGGGTTATGACATCGGATCAGGGATAAGCCACTATTGGATTGGGAAGTTCATCCCTATCACGATGTCACTTAGAAGGCTAGAAGTGCACTACCATGTAGTTTAGTTACCAAAGTGAAAAATAAATTCAAAGTTCACCCAATTAGAATTCTGACCATCGTTTAGGTTCTTAAAGTATGTATTGATCAAATCATAGATGAATGTCTAGAAATTAAACTAGCATTGATATTGATACTTAGATCATGAAGCTAAAAAACAGTGGATTATATATATATATATCTGTATGTTTTTGAAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAAACATTTAACTACATTTTCTTCTATCCTATGGATGTTTTTGATGGGCTGTTATGGTGATGTTTGTGTTTGTTATGCGGTTGGTTGAATAGGAAAGAGCTAACTATGAATAGGGGGTGAGATTGATAATGTTTtCTTTTTTtATTACCCCTTAGGATGTTACACTTAATTACATATGATAAATGTATTAACTTTGATAATACAATGAATGATATTATGATCATGAGACGCTACATTGATGAACCAA

mRNA sequence

ATCCTTTCTAACGCAATCCCCATTCAGAATCCGCCAAGTTTCTCTGCAACAAAAATCTTCATCGCCAACCACGATCTCCAAAAACCCATTTTCACAATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAAACATTTAACTACATTTTCTTCTATCCTATGGATGTTTTTGATGGGCTGTTATGGTGATGTTTGTGTTTGTTATGCGGTTGGTTGAATAGGAAAGAGCTAACTATGAATAGGGGGTGAGATTGATAATGTTTTCTTTTTTTATTACCCCTTAGGATGTTACACTTAATTACATATGATAAATGTATTAACTTTGATAATACAATGAATGATATTATGATCATGAGACGCTACATTGATGAACCAA

Coding sequence (CDS)

ATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAA

Protein sequence

MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI*

Homology

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BLAST of Cucsa.301890 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 7.7e-130
Identity = 242/348 (69.54%), Postives = 287/348 (82.47%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRD---ASPPSTA---NRNTKISVIGAGNVGMAIAQ 60
           M K+SS+S LG      S  FF+P+ D   A+P S+A    R TKISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+L DE+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRISG-TDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLA 300
               +++  LE I + V+GGAYEVI LKGYTSWAIGYSVASLA S+LRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTI 342
            GF+GI DG ++FLSLPA+LGRGG+LG+  + LT  E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

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BLAST of Cucsa.301890 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 6.7e-126
Identity = 230/342 (67.25%), Postives = 279/342 (81.58%), Query Frame = 1


Query: 16  DLSQAFFKPIRDASPPSTAN------RNTKISVIGAGNVGMAIAQTILTQDLVDELVLVD 75
           D S  FF+ + D  P +  +      R TK+SVIGAGNVGMAIAQTILTQ+L DE+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKETLEKIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++    +++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI-DGGDLFLS 315
            V+GGAYEVI LKGYTSWAIGYSVASLA S+LRDQRR+HPVSVLA GF+GI DG ++FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           LPA+LGR GVLG+  + LT  E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

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BLAST of Cucsa.301890 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.0e-122
Identity = 235/355 (66.20%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIR-DASPPSTAN---RNTKISVIGAGNVGMAIAQTI 60
           M+K++S+S LG    D S  FF+P+  D+S P++ +   R TK+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DL DE+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTY+AWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKG 300
             +++E LE I + V+  AYEVI+LKGYTSWAIGYSVASLA S+LRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           F+GI DG     + P +  R    G   + LT EE+KRLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

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BLAST of Cucsa.301890 vs. Swiss-Prot
Match: LDHA_MOUSE (L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3)

HSP 1 Score: 347.4 bits (890), Expect = 1.8e-94
Identity = 174/310 (56.13%), Postives = 228/310 (73.55%), Query Frame = 1


Query: 39  KISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DL DEL LVD   DKL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVD 158
           DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP   LLIVSNPVD
Sbjct: 82  DYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPVD 141

Query: 159 VLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTY+AWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLKNQQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLAR 278
           S ++V GV + S         +KE  +++HK+V+  AYEVI LKGYTSWAIG SVA LA 
Sbjct: 202 SGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAE 261

Query: 279 SILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSA 338
           SI+++ RR+HP+S + KG YGI+  D+FLS+P  LG+ G+  +  V LT EE  RL+ SA
Sbjct: 262 SIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSA 321

Query: 339 NTILQVQTEL 349
           +T+  +Q EL
Sbjct: 322 DTLWGIQKEL 330

[Close]

BLAST of Cucsa.301890 vs. Swiss-Prot
Match: LDHA_RAT (L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.9e-94
Identity = 176/312 (56.41%), Postives = 231/312 (74.04%), Query Frame = 1


Query: 39  KISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DL DEL LVD   DKL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVD 158
           DYS+TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIVSNPVD
Sbjct: 82  DYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPVD 141

Query: 159 VLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTY+AWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+      +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLKNQQIG--YEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASL 278
           S ++V GV + S   N Q+G   +KE  + +HK+V+  AYEVI LKGYTSWAIG SVA L
Sbjct: 202 SGVNVAGVSLKSL--NPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADL 261

Query: 279 ARSILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRD 338
           A SI+++ RR+HP+S + KG YGI   D+FLS+P  LG+ G+  +  V LT +E  RL+ 
Sbjct: 262 AESIMKNLRRVHPISTMIKGLYGIK-EDVFLSVPCILGQNGISDVVKVTLTPDEEARLKK 321

Query: 339 SANTILQVQTEL 349
           SA+T+  +Q EL
Sbjct: 322 SADTLWGIQKEL 330

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BLAST of Cucsa.301890 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 3.0e-181
Identity = 333/350 (95.14%), Postives = 338/350 (96.57%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGDLFLSLPAQLGRGGVLGITNVHLT EESKRLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Cucsa.301890 vs. TrEMBL
Match: A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 5.8e-169
Identity = 301/350 (86.00%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVINLKGYTSWAIGYS A+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TNVHLT EE++RLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Cucsa.301890 vs. TrEMBL
Match: D7TN36_VITVI (L-lactate dehydrogenase OS=Vitis vinifera GN=VIT_02s0154g00350 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 7.9e-166
Identity = 295/350 (84.29%), Postives = 323/350 (92.29%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGPGGLDL+QAFFKPI+  +PPS   R+TK+SVIG GNVGMAIAQTILTQD
Sbjct: 1   MHKSGSASSLGPGGLDLTQAFFKPIQQTAPPSPTKRHTKVSVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDEL LVD   DKLRGEMLDLQHAAAFLPRTKI AS DY+ITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELALVDVNADKLRGEMLDLQHAAAFLPRTKIHASVDYAITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV+LF +IVPPL ++SP++ILLIVSNPVD+LTY+AWKLSGFPSNRV+GSGT
Sbjct: 121 ESRLNLLQRNVSLFSRIVPPLAKYSPDSILLIVSNPVDILTYVAWKLSGFPSNRVLGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVI+LKGYTSWAIGYS ASLARSILR+QRRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVISLKGYTSWAIGYSAASLARSILRNQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGD+FLSLPAQLGRGG+LG+TNVHLT EE++RLRDSA TIL+VQ +LG+
Sbjct: 301 EGGDVFLSLPAQLGRGGILGVTNVHLTDEEAQRLRDSAKTILEVQNQLGL 350

[Close]

BLAST of Cucsa.301890 vs. TrEMBL
Match: G7JW16_MEDTR (L-lactate dehydrogenase OS=Medicago truncatula GN=MTR_5g012390 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.8e-165
Identity = 298/350 (85.14%), Postives = 325/350 (92.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGP GLDL+Q+FFK I+ ASPPS   R+ KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MHKSISGSSLGPDGLDLTQSFFKSIQHASPPSPTKRHNKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDA PDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LVDELVLVDAIPDKLRGEMLDLQHAAAFLPRTKIQASTDYSVTMGSDLCIVTAGARQING 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV++SP  +LLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFKKIIPPLVRYSPHCVLLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KE LE IHKEVI GAYEVI+LKGYTSWAIGYSVA+LAR+I+R+QR+IHPVSVLAKGFYGI
Sbjct: 241 KEMLENIHKEVINGAYEVISLKGYTSWAIGYSVANLARTIMRNQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++F+SLPAQLGRGGVLG+TN+H+T EE KRLRDSA TIL++Q +LGI
Sbjct: 301 DGGEVFISLPAQLGRGGVLGVTNIHMTEEEMKRLRDSAITILELQNQLGI 350

[Close]

BLAST of Cucsa.301890 vs. TrEMBL
Match: A0A0D2UW00_GOSRA (L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.3e-165
Identity = 293/350 (83.71%), Postives = 325/350 (92.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS+  R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTY++WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK V+  AYEVI+LKGYTSWAIGYSVA+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Cucsa.301890 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 552.0 bits (1421), Expect = 2.7e-157
Identity = 268/347 (77.23%), Postives = 313/347 (90.20%), Query Frame = 1


Query: 4   SSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVD 63
           +SS+  LGP GLDL+ AFFKPI ++ P   +NR TK+SV+G GNVGMAIAQTILTQDL D
Sbjct: 7   TSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLAD 66

Query: 64  ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESR 123
           E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ  GESR
Sbjct: 67  EIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESR 126

Query: 124 LNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLD 183
           LNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTY+AWKLSGFP NRV+GSGTNLD
Sbjct: 127 LNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLGSGTNLD 186

Query: 184 SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKET 243
           SSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI YEK+T
Sbjct: 187 SSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQIAYEKQT 246

Query: 244 LEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGIDGG 303
           LE IH+ V+G AYEVI LKGYTSWAIGYSVA+LAR+ILRDQR+IHPV+VLA+GFYG+DGG
Sbjct: 247 LEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGFYGVDGG 306

Query: 304 DLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           D+FLSLPA LGR GV+ +TNVH+T EE+++L+ SA TIL++Q++LG+
Sbjct: 307 DVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

[Close]

BLAST of Cucsa.301890 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 671.0 bits (1730), Expect = 1.1e-189
Identity = 347/350 (99.14%), Postives = 348/350 (99.43%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI SGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIXSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NL+SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 181 NLNSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Cucsa.301890 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 665.6 bits (1716), Expect = 4.7e-188
Identity = 343/350 (98.00%), Postives = 346/350 (98.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASPPST+NRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 83  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 142

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 143 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 202

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 203 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 262

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 263 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 322

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI
Sbjct: 323 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 382

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI
Sbjct: 383 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 432

[Close]

BLAST of Cucsa.301890 vs. NCBI nr
Match: gi|778700014|ref|XP_011654796.1| (PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus])

HSP 1 Score: 642.5 bits (1656), Expect = 4.3e-181
Identity = 333/350 (95.14%), Postives = 338/350 (96.57%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGDLFLSLPAQLGRGGVLGITNVHLT EESKRLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Cucsa.301890 vs. NCBI nr
Match: gi|802581859|ref|XP_012069951.1| (PREDICTED: L-lactate dehydrogenase B [Jatropha curcas])

HSP 1 Score: 601.7 bits (1550), Expect = 8.4e-169
Identity = 301/350 (86.00%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVINLKGYTSWAIGYS A+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TNVHLT EE++RLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Cucsa.301890 vs. NCBI nr
Match: gi|225427017|ref|XP_002271251.1| (PREDICTED: L-lactate dehydrogenase A isoform X2 [Vitis vinifera])

HSP 1 Score: 591.3 bits (1523), Expect = 1.1e-165
Identity = 295/350 (84.29%), Postives = 323/350 (92.29%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGPGGLDL+QAFFKPI+  +PPS   R+TK+SVIG GNVGMAIAQTILTQD
Sbjct: 1   MHKSGSASSLGPGGLDLTQAFFKPIQQTAPPSPTKRHTKVSVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDEL LVD   DKLRGEMLDLQHAAAFLPRTKI AS DY+ITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELALVDVNADKLRGEMLDLQHAAAFLPRTKIHASVDYAITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV+LF +IVPPL ++SP++ILLIVSNPVD+LTY+AWKLSGFPSNRV+GSGT
Sbjct: 121 ESRLNLLQRNVSLFSRIVPPLAKYSPDSILLIVSNPVDILTYVAWKLSGFPSNRVLGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVI+LKGYTSWAIGYS ASLARSILR+QRRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVISLKGYTSWAIGYSAASLARSILRNQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGD+FLSLPAQLGRGG+LG+TNVHLT EE++RLRDSA TIL+VQ +LG+
Sbjct: 301 EGGDVFLSLPAQLGRGGILGVTNVHLTDEEAQRLRDSAKTILEVQNQLGL 350

The following BLAST results are available for this feature:

BLAST of Cucsa.301890 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LDHA_HORVU	7.7e-130	69.54	L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1	[more]
LDHB_HORVU	6.7e-126	67.25	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1	[more]
LDH_MAIZE	2.0e-122	66.20	L-lactate dehydrogenase OS=Zea mays PE=2 SV=1	[more]
LDHA_MOUSE	1.8e-94	56.13	L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3	[more]
LDHA_RAT	8.9e-94	56.41	L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1	[more]

BLAST of Cucsa.301890 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKU9_CUCSA	3.0e-181	95.14	L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1	[more]
A0A067KUG8_JATCU	5.8e-169	86.00	L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1	[more]
D7TN36_VITVI	7.9e-166	84.29	L-lactate dehydrogenase OS=Vitis vinifera GN=VIT_02s0154g00350 PE=3 SV=1	[more]
G7JW16_MEDTR	1.8e-165	85.14	L-lactate dehydrogenase OS=Medicago truncatula GN=MTR_5g012390 PE=3 SV=1	[more]
A0A0D2UW00_GOSRA	2.3e-165	83.71	L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1	[more]

BLAST of Cucsa.301890 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	2.7e-157	77.23	Lactate/malate dehydrogenase family protein	[more]

BLAST of Cucsa.301890 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778700018\|ref\|XP_004151663.2\|	1.1e-189	99.14	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
gi\|659073513\|ref\|XP_008437100.1\|	4.7e-188	98.00	PREDICTED: L-lactate dehydrogenase B [Cucumis melo]	[more]
gi\|778700014\|ref\|XP_011654796.1\|	4.3e-181	95.14	PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus]	[more]
gi\|802581859\|ref\|XP_012069951.1\|	8.4e-169	86.00	PREDICTED: L-lactate dehydrogenase B [Jatropha curcas]	[more]
gi\|225427017\|ref\|XP_002271251.1\|	1.1e-165	84.29	PREDICTED: L-lactate dehydrogenase A isoform X2 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001236	Lactate/malate_DH_N
IPR001557	L-lactate/malate_DH
IPR011304	L-lactate_DH
IPR015955	Lactate_DH/Glyco_Ohase_4_C
IPR016040	NAD(P)-bd_dom
IPR018177	L-lactate_DH_AS
IPR022383	Lactate/malate_DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0004459	L-lactate dehydrogenase activity
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0019752	carboxylic acid metabolic process
GO:0005975	carbohydrate metabolic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.301890.1	Cucsa.301890.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 38..177 score: 2.2
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 64..88 score: 3.5E-54coord: 205..218 score: 3.5E-54coord: 151..171 score: 3.5E-54coord: 175..193 score: 3.5E-54coord: 39..63 score: 3.5
IPR001557	L-lactate/malate dehydrogenase	PIR	PIRSF000102	Lac_mal_DH	coord: 34..350 score: 1.9E
IPR001557	L-lactate/malate dehydrogenase	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 10..348 score: 2.1E
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37
IPR011304	L-lactate dehydrogenase	TIGRFAMs	TIGR01771	TIGR01771	coord: 42..341 score: 6.8E
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	G3DSA:3.90.110.10		coord: 179..348 score: 1.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	unknown	SSF56327	LDH C-terminal domain-like	coord: 178..346 score: 1.05
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..178 score: 7.9
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 24..177 score: 4.37
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213 scor
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..343 score: 8.1
None	No IPR available	PANTHER	PTHR11540:SF3	L-LACTATE DEHYDROGENASE	coord: 10..348 score: 2.1E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.301890	Cla011577	Watermelon (97103) v1	cgywmB552
Cucsa.301890	Csa5G161910	Cucumber (Chinese Long) v2	cgycuB434
Cucsa.301890	MELO3C005539	Melon (DHL92) v3.5.1	cgymeB495
Cucsa.301890	ClCG01G003970	Watermelon (Charleston Gray)	cgywcgB520
Cucsa.301890	CSPI05G06740	Wild cucumber (PI 183967)	cgycpiB457
Cucsa.301890	CmaCh15G011280	Cucurbita maxima (Rimu)	cgycmaB0830
Cucsa.301890	CmaCh02G015450	Cucurbita maxima (Rimu)	cgycmaB0835
Cucsa.301890	CmoCh15G011870	Cucurbita moschata (Rifu)	cgycmoB0824
Cucsa.301890	Lsi09G004170	Bottle gourd (USVL1VR-Ls)	cgylsiB487
Cucsa.301890	Cp4.1LG13g02350	Cucurbita pepo (Zucchini)	cgycpeB0789
Cucsa.301890	MELO3C005539.2	Melon (DHL92) v3.6.1	cgymedB494
Cucsa.301890	CsaV3_5G004150	Cucumber (Chinese Long) v3	cgycucB470
Cucsa.301890	Cla97C01G004140	Watermelon (97103) v2	cgywmbB520
Cucsa.301890	Bhi12G000410	Wax gourd	cgywgoB604
Cucsa.301890	Carg23210	Silver-seed gourd	carcgyB0621
Cucsa.301890	Carg02443	Silver-seed gourd	carcgyB1045

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.301890	Cucumber (Chinese Long) v3	cgycucB465
Cucsa.301890	Watermelon (97103) v2	cgywmbB521
Cucsa.301890	Cucumber (Gy14) v1	cgycgyB141
Cucsa.301890	Cucurbita maxima (Rimu)	cgycmaB0839
Cucsa.301890	Cucurbita moschata (Rifu)	cgycmoB0830
Cucsa.301890	Cucurbita moschata (Rifu)	cgycmoB0834
Cucsa.301890	Wild cucumber (PI 183967)	cgycpiB452
Cucsa.301890	Cucumber (Chinese Long) v2	cgycuB429
Cucsa.301890	Melon (DHL92) v3.5.1	cgymeB498
Cucsa.301890	Watermelon (Charleston Gray)	cgywcgB519
Cucsa.301890	Watermelon (97103) v1	cgywmB547
Cucsa.301890	Cucurbita pepo (Zucchini)	cgycpeB0794
Cucsa.301890	Cucurbita pepo (Zucchini)	cgycpeB0796
Cucsa.301890	Bottle gourd (USVL1VR-Ls)	cgylsiB491