Cucsa.301890.1 (mRNA) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.301890.1
Type	mRNA
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	L-lactate dehydrogenase
Location	scaffold02951 : 123600 .. 125959 (+)
Sequence length	1393

Genome Viewer

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCCTTTCTAACGCAATCCCCATTCAGAATCCGCCAAGTTTCTCTGCAACAAAAATCTTCATCGCCAACCACGATCTCCAAAAACCCATTTTCACAATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGTCAGTTTTGCCATTGATCATTGTTTTTTTtGGTTAATTTGGCGTTGTTTTACTGAAGAAATTTCCCTTTCGTTTGAATTGTTTTTGGTTGATTGGAATCAAATGAGTTGATCATTTTTGGGAAAAAGTAACCAAAATGAACCATAAAATTTCATATTTCTAATTTTGTTATATGAAGTTATTAGGCTCTTTCTAAAGTTCATAAATCAAATTAAAAGCTTTTAAGTGTAAGGAATTATCCATACAAACCAAATTATGTGTAGAGTAATTTATAACTTAACTCAAAAAAaTTAACTTTCACTTATAAATTTGGACTTATGTGATTTGGAGAAAGTTTGTTACTGTGTTTATAATTAAAACCCTAACAAATATGAGAGCTTTTTGTACTGATAACGGTTTCTTGTGTGGGGTGATTACAATAAGTACTCATTTATAACAACATTTTCAAAATGGTAAATACAACAAGTATGTTGTTAATCTATCAATATTTACAATATAGTAACTCCAACTGTTTTTGTTGTATTTACAATTTTTGAAAATTATTGCTATATAGGTTAATACTTTAAATTTAATTGTTATGTTTGTAATTGTTTGACCCACACCTGCAATAGTGTGAAGTTGGGATAGGGATTTGGCAATGTTAAATGGGAAAAGTGTAAAAAAaGATATCACATCGGGTTATGACATCGGATCAGGGATAAGCCACTATTGGATTGGGAAGTTCATCCCTATCACGATGTCACTTAGAAGGCTAGAAGTGCACTACCATGTAGTTTAGTTACCAAAGTGAAAAATAAATTCAAAGTTCACCCAATTAGAATTCTGACCATCGTTTAGGTTCTTAAAGTATGTATTGATCAAATCATAGATGAATGTCTAGAAATTAAACTAGCATTGATATTGATACTTAGATCATGAAGCTAAAAAACAGTGGATTATATATATATATATCTGTATGTTTTTGAAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAAACATTTAACTACATTTTCTTCTATCCTATGGATGTTTTTGATGGGCTGTTATGGTGATGTTTGTGTTTGTTATGCGGTTGGTTGAATAGGAAAGAGCTAACTATGAATAGGGGGTGAGATTGATAATGTTTtCTTTTTTtATTACCCCTTAGGATGTTACACTTAATTACATATGATAAATGTATTAACTTTGATAATACAATGAATGATATTATGATCATGAGACGCTACATTGATGAACCAA

mRNA sequence

ATCCTTTCTAACGCAATCCCCATTCAGAATCCGCCAAGTTTCTCTGCAACAAAAATCTTCATCGCCAACCACGATCTCCAAAAACCCATTTTCACAATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAAACATTTAACTACATTTTCTTCTATCCTATGGATGTTTTTGATGGGCTGTTATGGTGATGTTTGTGTTTGTTATGCGGTTGGTTGAATAGGAAAGAGCTAACTATGAATAGGGGGTGAGATTGATAATGTTTTCTTTTTTTATTACCCCTTAGGATGTTACACTTAATTACATATGATAAATGTATTAACTTTGATAATACAATGAATGATATTATGATCATGAGACGCTACATTGATGAACCAA

Coding sequence (CDS)

ATGCAGAAAAGTTCCTCAATCTCTTCCTTAGGTCCCGGCGGCCTCGACCTTTCTCAAGCCTTCTTCAAACCAATTCGTGACGCCTCTCCCCCATCCACAGCAAATCGCAACACCAAAATCTCCGTCATCGGCGCCGGAAACGTTGGTATGGCCATCGCTCAAACCATCCTCACTCAAGATCTGGTCGACGAACTCGTCCTCGTCGACGCCAAGCCCGACAAACTCCGTGGCGAAATGCTAGATCTCCAACATGCTGCTGCTTTTCTCCCTCGTACAAAAATTAGCGCCTCCACTGATTACTCCATTACTGCTGGCTCTGATCTGTGTATTGTTACTGCTGGTGCTCGGCAGATCGCCGGTGAATCTAGGTTGAATCTGTTGCAGAGGAATGTTGCTCTGTTTCAGAAGATCGTTCCGCCCTTGGTTCAGTTCTCTCCTGAGACGATTCTTTTGATTGTTTCGAATCCGGTGGATGTTTTGACTTATATTGCTTGGAAACTCTCTGGTTTTCCGTCGAATCGTGTGATTGGATCGGGAACGAATCTGGATTCGTCTAGGTTTCGGTTTCTTATTGCGGATCATCTTGACGTTAACGCTCAGGATGTTCAGGCATACATAGTTGGAGAGCATGGCGACAGCTCGGTGGCGCTATGGTCGAGCATAAGTGTGGGCGGGGTGCCGATCCTAAGCTTTCTAAAGAATCAACAAATCGGCTACGAAAAAGAAACATTAGAGAAAATTCACAAAGAGGTTATAGGTGGGGCTTACGAAGTCATAAACCTTAAGGGCTACACATCATGGGCGATCGGGTACTCCGTTGCGAGTTTAGCTCGGTCGATCCTTCGAGACCAAAGGAGGATCCATCCTGTCTCTGTTCTCGCAAAAGGATTTTACGGTATTGATGGTGGCGACTTGTTCCTTAGCCTGCCTGCACAACTTGGGAGGGGTGGAGTATTGGGGATCACCAATGTGCATCTAACGGCAGAGGAATCGAAACGGTTGAGGGACTCGGCAAACACCATCTTGCAGGTGCAAACCGAGTTGGGGATTTAA

Protein sequence

MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI*

Homology

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BLAST of Cucsa.301890.1 vs. Swiss-Prot
Match: LDHA_HORVU (L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 7.7e-130
Identity = 242/348 (69.54%), Postives = 287/348 (82.47%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRD---ASPPSTA---NRNTKISVIGAGNVGMAIAQ 60
           M K+SS+S LG      S  FF+P+ D   A+P S+A    R TKISVIGAGNVGMAIAQ
Sbjct: 1   MHKASSLSELGFDAGGASSGFFRPVADGCPATPTSSAVPHRRLTKISVIGAGNVGMAIAQ 60

Query: 61  TILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAG 120
           TILTQ+L DE+ LVDA PDKLRGE LDLQHAAAFLPR +IS  TD ++T  SDL IVTAG
Sbjct: 61  TILTQNLADEIALVDALPDKLRGEALDLQHAAAFLPRVRISG-TDAAVTKNSDLVIVTAG 120

Query: 121 ARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNR 180
           ARQI GE+RLNLLQRNVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++R
Sbjct: 121 ARQIPGETRLNLLQRNVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASR 180

Query: 181 VIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKN 240
           VIGSGTNLDSSRFRFLIA+HLDVNAQDVQAY+VGEHGDSSVA+WSSISVGG+P    L++
Sbjct: 181 VIGSGTNLDSSRFRFLIAEHLDVNAQDVQAYMVGEHGDSSVAIWSSISVGGMPAFKSLRD 240

Query: 241 QQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLA 300
               +++  LE I + V+GGAYEVI LKGYTSWAIGYSVASLA S+LRDQRR+HPVSVLA
Sbjct: 241 SHRSFDEAALEGIRRAVVGGAYEVIGLKGYTSWAIGYSVASLAASLLRDQRRVHPVSVLA 300

Query: 301 KGFYGI-DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTI 342
            GF+GI DG ++FLSLPA+LGRGG+LG+  + LT  E+ +LR SA T+
Sbjct: 301 SGFHGISDGHEVFLSLPARLGRGGILGVAEMDLTEAEAAQLRRSAKTL 347

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BLAST of Cucsa.301890.1 vs. Swiss-Prot
Match: LDHB_HORVU (L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 6.7e-126
Identity = 230/342 (67.25%), Postives = 279/342 (81.58%), Query Frame = 1


Query: 16  DLSQAFFKPIRDASPPSTAN------RNTKISVIGAGNVGMAIAQTILTQDLVDELVLVD 75
           D S  FF+ + D  P +  +      R TK+SVIGAGNVGMAIAQTILTQ+L DE+ LVD
Sbjct: 3   DASSGFFRAVADGCPITHTSCSAPHRRLTKVSVIGAGNVGMAIAQTILTQNLADEIALVD 62

Query: 76  AKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESRLNLLQR 135
           A PDKLRGE LDLQHAAAFLPR +I + TD ++T  SDL +VTAGARQI GE+RLNLLQR
Sbjct: 63  ALPDKLRGEALDLQHAAAFLPRVRIVSGTDAAVTKNSDLIVVTAGARQIPGETRLNLLQR 122

Query: 136 NVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRF 195
           NVAL++KIVPP+ + SP+ +LL+VSNPVDVLTY+AWKLSGFP++RVIGSGTNLDSSRFRF
Sbjct: 123 NVALYRKIVPPVAEHSPDALLLVVSNPVDVLTYVAWKLSGFPASRVIGSGTNLDSSRFRF 182

Query: 196 LIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKETLEKIHK 255
           L+A+HLDV+AQDVQAY+VGEHGDSSVA+WSSISVGG+P L  L++    +++  LE I +
Sbjct: 183 LVAEHLDVSAQDVQAYMVGEHGDSSVAIWSSISVGGMPALKSLRDSHRSFDEAALEGIRR 242

Query: 256 EVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI-DGGDLFLS 315
            V+GGAYEVI LKGYTSWAIGYSVASLA S+LRDQRR+HPVSVLA GF+GI DG ++FLS
Sbjct: 243 AVVGGAYEVIGLKGYTSWAIGYSVASLATSLLRDQRRVHPVSVLAAGFHGISDGHEVFLS 302

Query: 316 LPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           LPA+LGR GVLG+  + LT  E+ +LR SA T+ +    LG+
Sbjct: 303 LPARLGRAGVLGVAEMDLTEAEAAQLRRSAKTLWENCQLLGL 344

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BLAST of Cucsa.301890.1 vs. Swiss-Prot
Match: LDH_MAIZE (L-lactate dehydrogenase OS=Zea mays PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.0e-122
Identity = 235/355 (66.20%), Postives = 285/355 (80.28%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIR-DASPPSTAN---RNTKISVIGAGNVGMAIAQTI 60
           M+K++S+S LG    D S  FF+P+  D+S P++ +   R TK+SVIGAGNVGMAIAQTI
Sbjct: 1   MKKATSLSELGFDAGDASSGFFRPVSGDSSTPTSQHHRRRLTKVSVIGAGNVGMAIAQTI 60

Query: 61  LTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGAR 120
           LT+DL DE+ LVDA PDKLRGEMLDLQHAAAFLPRT++ + TD S+T GSDL IVTAGAR
Sbjct: 61  LTRDLADEIALVDAVPDKLRGEMLDLQHAAAFLPRTRLVSGTDMSVTRGSDLVIVTAGAR 120

Query: 121 QIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI 180
           QI GE+RL+LLQRNVALF+KIVPPL + S + +LL+VSNPVDVLTY+AWKLSGFP++RVI
Sbjct: 121 QIQGETRLDLLQRNVALFRKIVPPLAEQSHDALLLVVSNPVDVLTYVAWKLSGFPASRVI 180

Query: 181 GSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQ 240
           GSGTNLDSSRFRFL+A+HLDVNAQDVQAY+VGEHGDSSVA+WSS+SV G+P+L  L+   
Sbjct: 181 GSGTNLDSSRFRFLLAEHLDVNAQDVQAYMVGEHGDSSVAVWSSVSVAGMPVLKSLQESH 240

Query: 241 IGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKG 300
             +++E LE I + V+  AYEVI+LKGYTSWAIGYSVASLA S+LRDQRRIHPVSVLA+G
Sbjct: 241 RCFDEEALEGIRRAVVDSAYEVISLKGYTSWAIGYSVASLAASLLRDQRRIHPVSVLARG 300

Query: 301 FYGI-DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           F+GI DG     + P +  R    G   + LT EE+KRLR SA TI +    LG+
Sbjct: 301 FHGIPDGTTSSSACPPRRPRRRP-GRREMELTEEEAKRLRRSAKTIWENCQLLGL 354

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BLAST of Cucsa.301890.1 vs. Swiss-Prot
Match: LDHA_MOUSE (L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3)

HSP 1 Score: 347.4 bits (890), Expect = 1.8e-94
Identity = 174/310 (56.13%), Postives = 228/310 (73.55%), Query Frame = 1


Query: 39  KISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DL DEL LVD   DKL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVMEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVD 158
           DY +TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP   LLIVSNPVD
Sbjct: 82  DYCVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNIVKYSPHCKLLIVSNPVD 141

Query: 159 VLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTY+AWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+A     +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLKNQQIGYEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLAR 278
           S ++V GV + S         +KE  +++HK+V+  AYEVI LKGYTSWAIG SVA LA 
Sbjct: 202 SGVNVAGVSLKSLNPELGTDADKEQWKEVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAE 261

Query: 279 SILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSA 338
           SI+++ RR+HP+S + KG YGI+  D+FLS+P  LG+ G+  +  V LT EE  RL+ SA
Sbjct: 262 SIMKNLRRVHPISTMIKGLYGIN-EDVFLSVPCILGQNGISDVVKVTLTPEEEARLKKSA 321

Query: 339 NTILQVQTEL 349
           +T+  +Q EL
Sbjct: 322 DTLWGIQKEL 330

[Close]

BLAST of Cucsa.301890.1 vs. Swiss-Prot
Match: LDHA_RAT (L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.9e-94
Identity = 176/312 (56.41%), Postives = 231/312 (74.04%), Query Frame = 1


Query: 39  KISVIGAGNVGMAIAQTILTQDLVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISAST 98
           KI+V+G G VGMA A +IL +DL DEL LVD   DKL+GEM+DLQH + FL   KI +S 
Sbjct: 22  KITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSSK 81

Query: 99  DYSITAGSDLCIVTAGARQIAGESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVD 158
           DYS+TA S L I+TAGARQ  GESRLNL+QRNV +F+ I+P +V++SP+  LLIVSNPVD
Sbjct: 82  DYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPVD 141

Query: 159 VLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALW 218
           +LTY+AWK+SGFP NRVIGSG NLDS+RFR+L+ + L V+      +++GEHGDSSV +W
Sbjct: 142 ILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVW 201

Query: 219 SSISVGGVPILSFLKNQQIG--YEKETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASL 278
           S ++V GV + S   N Q+G   +KE  + +HK+V+  AYEVI LKGYTSWAIG SVA L
Sbjct: 202 SGVNVAGVSLKSL--NPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADL 261

Query: 279 ARSILRDQRRIHPVSVLAKGFYGIDGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRD 338
           A SI+++ RR+HP+S + KG YGI   D+FLS+P  LG+ G+  +  V LT +E  RL+ 
Sbjct: 262 AESIMKNLRRVHPISTMIKGLYGIK-EDVFLSVPCILGQNGISDVVKVTLTPDEEARLKK 321

Query: 339 SANTILQVQTEL 349
           SA+T+  +Q EL
Sbjct: 322 SADTLWGIQKEL 330

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BLAST of Cucsa.301890.1 vs. TrEMBL
Match: A0A0A0KKU9_CUCSA (L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 3.0e-181
Identity = 333/350 (95.14%), Postives = 338/350 (96.57%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGDLFLSLPAQLGRGGVLGITNVHLT EESKRLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Cucsa.301890.1 vs. TrEMBL
Match: A0A067KUG8_JATCU (L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 5.8e-169
Identity = 301/350 (86.00%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVINLKGYTSWAIGYS A+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TNVHLT EE++RLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Cucsa.301890.1 vs. TrEMBL
Match: D7TN36_VITVI (L-lactate dehydrogenase OS=Vitis vinifera GN=VIT_02s0154g00350 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 7.9e-166
Identity = 295/350 (84.29%), Postives = 323/350 (92.29%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGPGGLDL+QAFFKPI+  +PPS   R+TK+SVIG GNVGMAIAQTILTQD
Sbjct: 1   MHKSGSASSLGPGGLDLTQAFFKPIQQTAPPSPTKRHTKVSVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDEL LVD   DKLRGEMLDLQHAAAFLPRTKI AS DY+ITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELALVDVNADKLRGEMLDLQHAAAFLPRTKIHASVDYAITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV+LF +IVPPL ++SP++ILLIVSNPVD+LTY+AWKLSGFPSNRV+GSGT
Sbjct: 121 ESRLNLLQRNVSLFSRIVPPLAKYSPDSILLIVSNPVDILTYVAWKLSGFPSNRVLGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVI+LKGYTSWAIGYS ASLARSILR+QRRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVISLKGYTSWAIGYSAASLARSILRNQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGD+FLSLPAQLGRGG+LG+TNVHLT EE++RLRDSA TIL+VQ +LG+
Sbjct: 301 EGGDVFLSLPAQLGRGGILGVTNVHLTDEEAQRLRDSAKTILEVQNQLGL 350

[Close]

BLAST of Cucsa.301890.1 vs. TrEMBL
Match: G7JW16_MEDTR (L-lactate dehydrogenase OS=Medicago truncatula GN=MTR_5g012390 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.8e-165
Identity = 298/350 (85.14%), Postives = 325/350 (92.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGP GLDL+Q+FFK I+ ASPPS   R+ KISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MHKSISGSSLGPDGLDLTQSFFKSIQHASPPSPTKRHNKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDA PDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LVDELVLVDAIPDKLRGEMLDLQHAAAFLPRTKIQASTDYSVTMGSDLCIVTAGARQING 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV++SP  +LLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFKKIIPPLVRYSPHCVLLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KE LE IHKEVI GAYEVI+LKGYTSWAIGYSVA+LAR+I+R+QR+IHPVSVLAKGFYGI
Sbjct: 241 KEMLENIHKEVINGAYEVISLKGYTSWAIGYSVANLARTIMRNQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++F+SLPAQLGRGGVLG+TN+H+T EE KRLRDSA TIL++Q +LGI
Sbjct: 301 DGGEVFISLPAQLGRGGVLGVTNIHMTEEEMKRLRDSAITILELQNQLGI 350

[Close]

BLAST of Cucsa.301890.1 vs. TrEMBL
Match: A0A0D2UW00_GOSRA (L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.3e-165
Identity = 293/350 (83.71%), Postives = 325/350 (92.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDL+QAFFKPI++  PPS+  R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASSLGPGGLDLAQAFFKPIQNCDPPSSTKRHTKISVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAK DKLRGEMLDLQHAAAFLPRTKI+AS DYS+TAGSDLCIVTAGARQ  G
Sbjct: 61  LADELALVDAKSDKLRGEMLDLQHAAAFLPRTKINASVDYSVTAGSDLCIVTAGARQNPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF  I+PPL ++SP++ILLIVSNPVDVLTY++WKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFSNIIPPLAKYSPDSILLIVSNPVDVLTYVSWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSIS+GGVP+LSFLKNQQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISIGGVPVLSFLKNQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           K+TLE IHK V+  AYEVI+LKGYTSWAIGYSVA+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KQTLENIHKAVVESAYEVISLKGYTSWAIGYSVANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TN+HLT EE +RLR SA TIL+VQ++LG+
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNIHLTDEEVQRLRKSAETILEVQSQLGL 350

[Close]

BLAST of Cucsa.301890.1 vs. TAIR10
Match: AT4G17260.1 (AT4G17260.1 Lactate/malate dehydrogenase family protein)

HSP 1 Score: 552.0 bits (1421), Expect = 2.7e-157
Identity = 268/347 (77.23%), Postives = 313/347 (90.20%), Query Frame = 1


Query: 4   SSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQDLVD 63
           +SS+  LGP GLDL+ AFFKPI ++ P   +NR TK+SV+G GNVGMAIAQTILTQDL D
Sbjct: 7   TSSLKDLGPSGLDLTSAFFKPIHNSDPSLPSNRRTKVSVVGVGNVGMAIAQTILTQDLAD 66

Query: 64  ELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAGESR 123
           E+ LVDAKPDKLRGEMLDLQHAAAFLPRTKI+AS DY +TAGSDLCIVTAGARQ  GESR
Sbjct: 67  EIALVDAKPDKLRGEMLDLQHAAAFLPRTKITASVDYEVTAGSDLCIVTAGARQNPGESR 126

Query: 124 LNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLD 183
           LNLLQRNVALF+ I+PPL + SP++IL+IVSNPVDVLTY+AWKLSGFP NRV+GSGTNLD
Sbjct: 127 LNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVDVLTYVAWKLSGFPVNRVLGSGTNLD 186

Query: 184 SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYEKET 243
           SSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGG+P+LSFL+  QI YEK+T
Sbjct: 187 SSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGIPVLSFLEKNQIAYEKQT 246

Query: 244 LEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGIDGG 303
           LE IH+ V+G AYEVI LKGYTSWAIGYSVA+LAR+ILRDQR+IHPV+VLA+GFYG+DGG
Sbjct: 247 LEDIHQAVVGSAYEVIGLKGYTSWAIGYSVANLARTILRDQRKIHPVTVLARGFYGVDGG 306

Query: 304 DLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           D+FLSLPA LGR GV+ +TNVH+T EE+++L+ SA TIL++Q++LG+
Sbjct: 307 DVFLSLPALLGRNGVVAVTNVHMTDEEAEKLQKSAKTILEMQSQLGL 353

[Close]

BLAST of Cucsa.301890.1 vs. NCBI nr
Match: gi|778700018|ref|XP_004151663.2| (PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus])

HSP 1 Score: 671.0 bits (1730), Expect = 1.1e-189
Identity = 347/350 (99.14%), Postives = 348/350 (99.43%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVT GARQIAG
Sbjct: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTGGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVI SGT
Sbjct: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIXSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NL+SSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 181 NLNSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI
Sbjct: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI
Sbjct: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 350

[Close]

BLAST of Cucsa.301890.1 vs. NCBI nr
Match: gi|659073513|ref|XP_008437100.1| (PREDICTED: L-lactate dehydrogenase B [Cucumis melo])

HSP 1 Score: 665.6 bits (1716), Expect = 4.7e-188
Identity = 343/350 (98.00%), Postives = 346/350 (98.86%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASPPST+NRNTKISVIGAGNVGMAIAQTILTQD
Sbjct: 83  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPPSTSNRNTKISVIGAGNVGMAIAQTILTQD 142

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYS TAGS+LCIVTAGARQIAG
Sbjct: 143 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSTTAGSNLCIVTAGARQIAG 202

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTY+AWKLSGFPSNRVIGSGT
Sbjct: 203 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYVAWKLSGFPSNRVIGSGT 262

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQI YE
Sbjct: 263 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIAYE 322

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI
Sbjct: 323 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 382

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI
Sbjct: 383 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 432

[Close]

BLAST of Cucsa.301890.1 vs. NCBI nr
Match: gi|778700014|ref|XP_011654796.1| (PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus])

HSP 1 Score: 642.5 bits (1656), Expect = 4.3e-181
Identity = 333/350 (95.14%), Postives = 338/350 (96.57%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S SSLGPGGLDLSQAFFKPIRDASP ST+N NTKISVIG GNVGMAIAQTILTQD
Sbjct: 63  MQKSPSTSSLGPGGLDLSQAFFKPIRDASPLSTSNCNTKISVIGTGNVGMAIAQTILTQD 122

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITA SDLCIVTAGARQIAG
Sbjct: 123 LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITARSDLCIVTAGARQIAG 182

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV LFQKIVP LVQFSPETILLIVSNPVDVLTY+AWKLSGF SNRVIGSGT
Sbjct: 183 ESRLNLLQRNVTLFQKIVPLLVQFSPETILLIVSNPVDVLTYVAWKLSGFSSNRVIGSGT 242

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE
Sbjct: 243 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 302

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSV+AKGFYGI
Sbjct: 303 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVIAKGFYGI 362

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGDLFLSLPAQLGRGGVLGITNVHLT EESKRLRDSANTIL VQ ELGI
Sbjct: 363 EGGDLFLSLPAQLGRGGVLGITNVHLTTEESKRLRDSANTILHVQAELGI 412

[Close]

BLAST of Cucsa.301890.1 vs. NCBI nr
Match: gi|802581859|ref|XP_012069951.1| (PREDICTED: L-lactate dehydrogenase B [Jatropha curcas])

HSP 1 Score: 601.7 bits (1550), Expect = 8.4e-169
Identity = 301/350 (86.00%), Postives = 328/350 (93.71%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           MQKS S S+LGPGGLDL+QAFFKPI++ +PPS   R+TKISVIG GNVGMAIAQTILTQD
Sbjct: 1   MQKSPSASTLGPGGLDLTQAFFKPIQNTAPPSPTKRHTKISVIGTGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           L DEL LVDAKPDKLRGEMLDLQHAAAFLPRTKI ASTDYS+T GSDLCIVTAGARQI G
Sbjct: 61  LADELALVDAKPDKLRGEMLDLQHAAAFLPRTKIIASTDYSVTVGSDLCIVTAGARQIPG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNVALF+KI+PPLV +SPE+IL+IVSNPVDVLTYIAWKLSGFPSNRVIGSGT
Sbjct: 121 ESRLNLLQRNVALFEKIIPPLVNYSPESILMIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQA+IVGEHGDSSVALWSSISVGGVPILSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAFIVGEHGDSSVALWSSISVGGVPILSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVINLKGYTSWAIGYS A+LARS+LRDQR+IHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVINLKGYTSWAIGYSAANLARSLLRDQRKIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           DGG++FLSLPAQLGRGGVLG+TNVHLT EE++RLR+SA T+L+VQ++LGI
Sbjct: 301 DGGEVFLSLPAQLGRGGVLGVTNVHLTEEEARRLRESAKTLLEVQSQLGI 350

[Close]

BLAST of Cucsa.301890.1 vs. NCBI nr
Match: gi|225427017|ref|XP_002271251.1| (PREDICTED: L-lactate dehydrogenase A isoform X2 [Vitis vinifera])

HSP 1 Score: 591.3 bits (1523), Expect = 1.1e-165
Identity = 295/350 (84.29%), Postives = 323/350 (92.29%), Query Frame = 1


Query: 1   MQKSSSISSLGPGGLDLSQAFFKPIRDASPPSTANRNTKISVIGAGNVGMAIAQTILTQD 60
           M KS S SSLGPGGLDL+QAFFKPI+  +PPS   R+TK+SVIG GNVGMAIAQTILTQD
Sbjct: 1   MHKSGSASSLGPGGLDLTQAFFKPIQQTAPPSPTKRHTKVSVIGVGNVGMAIAQTILTQD 60

Query: 61  LVDELVLVDAKPDKLRGEMLDLQHAAAFLPRTKISASTDYSITAGSDLCIVTAGARQIAG 120
           LVDEL LVD   DKLRGEMLDLQHAAAFLPRTKI AS DY+ITAGSDLCIVTAGARQIAG
Sbjct: 61  LVDELALVDVNADKLRGEMLDLQHAAAFLPRTKIHASVDYAITAGSDLCIVTAGARQIAG 120

Query: 121 ESRLNLLQRNVALFQKIVPPLVQFSPETILLIVSNPVDVLTYIAWKLSGFPSNRVIGSGT 180
           ESRLNLLQRNV+LF +IVPPL ++SP++ILLIVSNPVD+LTY+AWKLSGFPSNRV+GSGT
Sbjct: 121 ESRLNLLQRNVSLFSRIVPPLAKYSPDSILLIVSNPVDILTYVAWKLSGFPSNRVLGSGT 180

Query: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPILSFLKNQQIGYE 240
           NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVP+LSFL+ QQI YE
Sbjct: 181 NLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYE 240

Query: 241 KETLEKIHKEVIGGAYEVINLKGYTSWAIGYSVASLARSILRDQRRIHPVSVLAKGFYGI 300
           KETLE IHK VI  AYEVI+LKGYTSWAIGYS ASLARSILR+QRRIHPVSVLAKGFYGI
Sbjct: 241 KETLENIHKAVIDSAYEVISLKGYTSWAIGYSAASLARSILRNQRRIHPVSVLAKGFYGI 300

Query: 301 DGGDLFLSLPAQLGRGGVLGITNVHLTAEESKRLRDSANTILQVQTELGI 351
           +GGD+FLSLPAQLGRGG+LG+TNVHLT EE++RLRDSA TIL+VQ +LG+
Sbjct: 301 EGGDVFLSLPAQLGRGGILGVTNVHLTDEEAQRLRDSAKTILEVQNQLGL 350

The following BLAST results are available for this feature:

BLAST of Cucsa.301890.1 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LDHA_HORVU	7.7e-130	69.54	L-lactate dehydrogenase A OS=Hordeum vulgare PE=1 SV=1	[more]
LDHB_HORVU	6.7e-126	67.25	L-lactate dehydrogenase B (Fragment) OS=Hordeum vulgare PE=1 SV=1	[more]
LDH_MAIZE	2.0e-122	66.20	L-lactate dehydrogenase OS=Zea mays PE=2 SV=1	[more]
LDHA_MOUSE	1.8e-94	56.13	L-lactate dehydrogenase A chain OS=Mus musculus GN=Ldha PE=1 SV=3	[more]
LDHA_RAT	8.9e-94	56.41	L-lactate dehydrogenase A chain OS=Rattus norvegicus GN=Ldha PE=1 SV=1	[more]

BLAST of Cucsa.301890.1 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KKU9_CUCSA	3.0e-181	95.14	L-lactate dehydrogenase OS=Cucumis sativus GN=Csa_5G161920 PE=3 SV=1	[more]
A0A067KUG8_JATCU	5.8e-169	86.00	L-lactate dehydrogenase OS=Jatropha curcas GN=JCGZ_03382 PE=3 SV=1	[more]
D7TN36_VITVI	7.9e-166	84.29	L-lactate dehydrogenase OS=Vitis vinifera GN=VIT_02s0154g00350 PE=3 SV=1	[more]
G7JW16_MEDTR	1.8e-165	85.14	L-lactate dehydrogenase OS=Medicago truncatula GN=MTR_5g012390 PE=3 SV=1	[more]
A0A0D2UW00_GOSRA	2.3e-165	83.71	L-lactate dehydrogenase OS=Gossypium raimondii GN=B456_009G297700 PE=3 SV=1	[more]

BLAST of Cucsa.301890.1 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G17260.1	2.7e-157	77.23	Lactate/malate dehydrogenase family protein	[more]

BLAST of Cucsa.301890.1 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778700018\|ref\|XP_004151663.2\|	1.1e-189	99.14	PREDICTED: LOW QUALITY PROTEIN: L-lactate dehydrogenase A-like [Cucumis sativus]	[more]
gi\|659073513\|ref\|XP_008437100.1\|	4.7e-188	98.00	PREDICTED: L-lactate dehydrogenase B [Cucumis melo]	[more]
gi\|778700014\|ref\|XP_011654796.1\|	4.3e-181	95.14	PREDICTED: L-lactate dehydrogenase B-like [Cucumis sativus]	[more]
gi\|802581859\|ref\|XP_012069951.1\|	8.4e-169	86.00	PREDICTED: L-lactate dehydrogenase B [Jatropha curcas]	[more]
gi\|225427017\|ref\|XP_002271251.1\|	1.1e-165	84.29	PREDICTED: L-lactate dehydrogenase A isoform X2 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this mRNA:

Vocabulary: **INTERPRO**
Term	Definition
IPR001236	Lactate/malate_DH_N
IPR001557	L-lactate/malate_DH
IPR011304	L-lactate_DH
IPR015955	Lactate_DH/Glyco_Ohase_4_C
IPR016040	NAD(P)-bd_dom
IPR018177	L-lactate_DH_AS
IPR022383	Lactate/malate_DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0004459	L-lactate dehydrogenase activity
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process
GO:0019752	carboxylic acid metabolic process
GO:0005975	carbohydrate metabolic process

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0006094	gluconeogenesis
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004459	L-lactate dehydrogenase activity
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Cucsa.301890	Cucsa.301890	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Cucsa.301890.1	Cucsa.301890.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.301890.1.five_prime_UTR.1	Cucsa.301890.1.five_prime_UTR.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.301890.1.CDS.1	Cucsa.301890.1.CDS.1	CDS
Cucsa.301890.1.CDS.2	Cucsa.301890.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Cucsa.301890.1.three_prime_UTR.1	Cucsa.301890.1.three_prime_UTR.1	three_prime_UTR

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001236	Lactate/malate dehydrogenase, N-terminal	PFAM	PF00056	Ldh_1_N	coord: 38..177 score: 2.2
IPR001557	L-lactate/malate dehydrogenase	PRINTS	PR00086	LLDHDRGNASE	coord: 64..88 score: 3.5E-54coord: 205..218 score: 3.5E-54coord: 151..171 score: 3.5E-54coord: 175..193 score: 3.5E-54coord: 39..63 score: 3.5
IPR001557	L-lactate/malate dehydrogenase	PIR	PIRSF000102	Lac_mal_DH	coord: 34..350 score: 1.9E
IPR001557	L-lactate/malate dehydrogenase	PANTHER	PTHR11540	MALATE AND LACTATE DEHYDROGENASE	coord: 10..348 score: 2.1E
IPR011304	L-lactate dehydrogenase	HAMAP	MF_00488	Lactate_dehydrog	coord: 37..349 score: 37
IPR011304	L-lactate dehydrogenase	TIGRFAMs	TIGR01771	TIGR01771	coord: 42..341 score: 6.8E
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	GENE3D	G3DSA:3.90.110.10		coord: 179..348 score: 1.6
IPR015955	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	unknown	SSF56327	LDH C-terminal domain-like	coord: 178..346 score: 1.05
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 29..178 score: 7.9
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 24..177 score: 4.37
IPR018177	L-lactate dehydrogenase, active site	PROSITE	PS00064	L_LDH	coord: 207..213 scor
IPR022383	Lactate/malate dehydrogenase, C-terminal	PFAM	PF02866	Ldh_1_C	coord: 180..343 score: 8.1
None	No IPR available	PANTHER	PTHR11540:SF3	L-LACTATE DEHYDROGENASE	coord: 10..348 score: 2.1E