CSPI05G28510 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G28510
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Metalloendoproteinase 1, putative
Location	Chr5 : 26890085 .. 26890810 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTACATTTTATCTTCTATCCATATATAATTTTACGTGCATTTTTCATCTATATTACAACAATTTGGACTTTTTATGACAGAAAAATAAAGACAAATTAGGAGAGAGAAAAGTCTGCAATAAAACTTACAAAGAAAATAAACATTTGAACTATATTTTTCTATGCTCAACAGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

mRNA sequence

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

Coding sequence (CDS)

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTGGCACATTCGAGAGAGTTATTTTGAAACGATAA

Homology

[Close]

BLAST of CSPI05G28510 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 3.9e-24
Identity = 55/133 (41.35%), Postives = 81/133 (60.90%), Query Frame = 1


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F D +FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTD-DFDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTY 174
            P+WP ++  LTY
Sbjct: 168 EPRWPRNRRDLTY 177

[Close]

BLAST of CSPI05G28510 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 8.7e-24
Identity = 55/152 (36.18%), Postives = 88/152 (57.89%), Query Frame = 1


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
                 H H+V  Y  F G P+WP ++  LTY
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 173

[Close]

BLAST of CSPI05G28510 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.4e-20
Identity = 59/172 (34.30%), Postives = 88/172 (51.16%), Query Frame = 1


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTY 161

[Close]

BLAST of CSPI05G28510 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.1e-16
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 1


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 166

Query: 169 KSHLTY 174
           +  LTY
Sbjct: 184 RDTLTY 166

[Close]

BLAST of CSPI05G28510 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 81.6 bits (200), Expect = 9.6e-15
Identity = 60/170 (35.29%), Postives = 78/170 (45.88%), Query Frame = 1


Query: 4   ETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  VK Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 123
           H GY+ N     +  FDD+ FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCG  
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
           DI+    +  S              F  +S Y FF   P+W A  + LTY
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTY 152

[Close]

BLAST of CSPI05G28510 vs. TrEMBL
Match: A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 5.0e-95
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

[Close]

BLAST of CSPI05G28510 vs. TrEMBL
Match: A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.1e-60
Identity = 121/174 (69.54%), Postives = 141/174 (81.03%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYG
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

[Close]

BLAST of CSPI05G28510 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.7e-47
Identity = 108/179 (60.34%), Postives = 128/179 (71.51%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSS---HPKSH--LSNGLVFLKNLNGCKKGDKVEGI 60
           MA E L  F  T   L++  FPLISSS   H  SH   S+  VFL  L G +KGDKVEGI
Sbjct: 1   MALEELSFFTFT--LLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGI 60

Query: 61  HQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLM 120
           HQ+K+YL  FGYLN   Q +S++ +DDEFD +LE+AIKTYQ NYNLK TGT++A T+  M
Sbjct: 61  HQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQM 120

Query: 121 SRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           S+PRCG ADI+ G T M+S KK       I GHFHSVSH+AFFDGNPKWPA+KSHLTYG
Sbjct: 121 SKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 176

[Close]

BLAST of CSPI05G28510 vs. TrEMBL
Match: A0A067DG72_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.0e-39
Identity = 96/176 (54.55%), Postives = 123/176 (69.89%), Query Frame = 1


Query: 8   SFLTTSFFLVIVLFPLISSSHPKSHL--------SNGLVFLKNLNGCKKGDKVEGIHQVK 67
           +FL+ S F VI +  L   SH  S L        S+   F+++L GC KGDK++GIH++K
Sbjct: 5   AFLSFSIFAVIQIILLSLLSHNYSVLAHSHAEKKSSPFKFIEHLKGCHKGDKIKGIHELK 64

Query: 68  EYLQHFGYLN--NYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSR 127
           EY++ FGYLN  NY +N S   DDD+FD +LESAIKTYQLNY+LK+TG ++A T+  M+ 
Sbjct: 65  EYMEKFGYLNYDNY-KNQSTHADDDDFDELLESAIKTYQLNYHLKSTGVLDANTVAKMTI 124

Query: 128 PRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
           PRCG ADIV+G TRM+SGKK   QH   N HF +VSHY+FF  +PKWPASK  LTY
Sbjct: 125 PRCGVADIVNGTTRMRSGKKR-QQH---NHHFRTVSHYSFFPRSPKWPASKFSLTY 175

[Close]

BLAST of CSPI05G28510 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 2.5e-38
Identity = 84/163 (51.53%), Postives = 115/163 (70.55%), Query Frame = 1


Query: 17  VIVLFPLISS-----SHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNY 76
           +I+L  L+SS     SH K+  S+   FLK+L GC KGD ++GIH +K+YL++FGYL+  
Sbjct: 13  LILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLENFGYLSYK 72

Query: 77  VQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTR 136
            Q++S   +DD+FD +LE A+KTYQ NY+L  TG ++++T+  M  PRCG ADI++G TR
Sbjct: 73  NQSHS---NDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVADIINGTTR 132

Query: 137 MKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           M+S  K  N H   +  FH+VSHY FF GNP+WPASK HLTYG
Sbjct: 133 MQSSNK--NPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYG 170

[Close]

BLAST of CSPI05G28510 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 112.8 bits (281), Expect = 2.2e-25
Identity = 55/133 (41.35%), Postives = 81/133 (60.90%), Query Frame = 1


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F D +FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTD-DFDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKING-HFHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTY 174
            P+WP ++  LTY
Sbjct: 168 EPRWPRNRRDLTY 177

[Close]

BLAST of CSPI05G28510 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 111.7 bits (278), Expect = 4.9e-25
Identity = 55/152 (36.18%), Postives = 88/152 (57.89%), Query Frame = 1


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
                 H H+V  Y  F G P+WP ++  LTY
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTY 173

[Close]

BLAST of CSPI05G28510 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 99.8 bits (247), Expect = 1.9e-21
Identity = 59/172 (34.30%), Postives = 88/172 (51.16%), Query Frame = 1


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTY 174
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTY 161

[Close]

BLAST of CSPI05G28510 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 85.9 bits (211), Expect = 2.9e-17
Identity = 50/126 (39.68%), Postives = 67/126 (53.17%), Query Frame = 1


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSD----------------THMTINNDFLHTTAHYTYFNGKPKW--N 166

Query: 169 KSHLTY 174
           +  LTY
Sbjct: 184 RDTLTY 166

[Close]

BLAST of CSPI05G28510 vs. NCBI nr
Match: gi|449449417|ref|XP_004142461.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 355.1 bits (910), Expect = 7.2e-95
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 174

[Close]

BLAST of CSPI05G28510 vs. NCBI nr
Match: gi|659118019|ref|XP_008458907.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 329.7 bits (844), Expect = 3.2e-87
Identity = 158/174 (90.80%), Postives = 166/174 (95.40%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYG
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYG 174

[Close]

BLAST of CSPI05G28510 vs. NCBI nr
Match: gi|449449409|ref|XP_004142457.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-60
Identity = 121/174 (69.54%), Postives = 141/174 (81.03%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYG
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYG 171

[Close]

BLAST of CSPI05G28510 vs. NCBI nr
Match: gi|659117646|ref|XP_008458713.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 223.0 bits (567), Expect = 4.3e-55
Identity = 103/140 (73.57%), Postives = 123/140 (87.86%), Query Frame = 1


Query: 35  NGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKT 94
           +GLVFLKNLNGCKKGDKVEGIHQ+K+YLQHFGY+NN V  +SKS  DDEFD +LE A+KT
Sbjct: 2   DGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKS-SDDEFDEILEFAVKT 61

Query: 95  YQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSH 154
           YQ+NYNLKATGT++  T+  MS+PRCG ADI++G+T MKSGK++VN+HRKI GHFH VSH
Sbjct: 62  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 121

Query: 155 YAFFDGNPKWPASKSHLTYG 175
           +AFF+GN KWP SKSHLTYG
Sbjct: 122 FAFFEGNLKWPNSKSHLTYG 140

[Close]

BLAST of CSPI05G28510 vs. NCBI nr
Match: gi|449449419|ref|XP_004142462.1| (PREDICTED: metalloendoproteinase 1 [Cucumis sativus])

HSP 1 Score: 197.2 bits (500), Expect = 2.5e-47
Identity = 108/179 (60.34%), Postives = 128/179 (71.51%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSS---HPKSH--LSNGLVFLKNLNGCKKGDKVEGI 60
           MA E L  F  T   L++  FPLISSS   H  SH   S+  VFL  L G +KGDKVEGI
Sbjct: 1   MALEELSFFTFT--LLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGI 60

Query: 61  HQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLM 120
           HQ+K+YL  FGYLN   Q +S++ +DDEFD +LE+AIKTYQ NYNLK TGT++A T+  M
Sbjct: 61  HQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQM 120

Query: 121 SRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYG 175
           S+PRCG ADI+ G T M+S KK       I GHFHSVSH+AFFDGNPKWPA+KSHLTYG
Sbjct: 121 SKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYG 176

The following BLAST results are available for this feature:

BLAST of CSPI05G28510 vs. Swiss-Prot


Match Name	E-value	Identity	Description
3MMP_ARATH	3.9e-24	41.35	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]
2MMP_ARATH	8.7e-24	36.18	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
5MMP_ARATH	3.4e-20	34.30	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
1MMP_ARATH	5.1e-16	39.68	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]
MEP1_SOYBN	9.6e-15	35.29	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]

BLAST of CSPI05G28510 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU08_CUCSA	5.0e-95	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1	[more]
A0A0A0KS47_CUCSA	1.1e-60	69.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1	[more]
A0A0A0KS39_CUCSA	1.7e-47	60.34	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1	[more]
A0A067DG72_CITSI	3.0e-39	54.55	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1	[more]
B9RUG6_RICCO	2.5e-38	51.53	Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1	[more]

BLAST of CSPI05G28510 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G24140.1	2.2e-25	41.35	Matrixin family protein	[more]
AT1G70170.1	4.9e-25	36.18	matrix metalloproteinase	[more]
AT1G59970.1	1.9e-21	34.30	Matrixin family protein	[more]
AT4G16640.1	2.9e-17	39.68	Matrixin family protein	[more]

BLAST of CSPI05G28510 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449449417\|ref\|XP_004142461.1\|	7.2e-95	100.00	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659118019\|ref\|XP_008458907.1\|	3.2e-87	90.80	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449409\|ref\|XP_004142457.1\|	1.5e-60	69.54	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659117646\|ref\|XP_008458713.1\|	4.3e-55	73.57	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449419\|ref\|XP_004142462.1\|	2.5e-47	60.34	PREDICTED: metalloendoproteinase 1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002477	Peptidoglycan-bd-like
IPR024079	MetalloPept_cat_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0008237	metallopeptidase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0008150	biological_process
cellular_component	GO:0031012	extracellular matrix
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G28510.1	CSPI05G28510.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002477	Peptidoglycan binding-like	GENE3D	G3DSA:1.10.101.10		coord: 54..116 score: 1.9
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 53..115 score: 8.4
IPR002477	Peptidoglycan binding-like	unknown	SSF47090	PGBD-like	coord: 46..125 score: 1.73
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 117..173 score: 5.
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 150..174 score: 5.7E-47coord: 4..128 score: 5.7
None	No IPR available	PANTHER	PTHR10201:SF140	F20P5.11 PROTEIN	coord: 150..174 score: 5.7E-47coord: 4..128 score: 5.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G28510	Csa5G632130	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G28510	MELO3C021493	Melon (DHL92) v3.5.1	cpimeB349
CSPI05G28510	CmaCh04G027320	Cucurbita maxima (Rimu)	cmacpiB730
CSPI05G28510	Carg02276	Silver-seed gourd	carcpiB0297

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G28510	Wax gourd	cpiwgoB473
CSPI05G28510	Wax gourd	cpiwgoB505
CSPI05G28510	Wild cucumber (PI 183967)	cpicpiB036
CSPI05G28510	Cucumber (Gy14) v1	cgycpiB110
CSPI05G28510	Cucumber (Gy14) v1	cgycpiB236
CSPI05G28510	Cucurbita maxima (Rimu)	cmacpiB304
CSPI05G28510	Cucurbita moschata (Rifu)	cmocpiB216
CSPI05G28510	Cucurbita moschata (Rifu)	cmocpiB296
CSPI05G28510	Cucurbita moschata (Rifu)	cmocpiB722
CSPI05G28510	Cucumber (Chinese Long) v2	cpicuB219
CSPI05G28510	Melon (DHL92) v3.5.1	cpimeB382
CSPI05G28510	Watermelon (Charleston Gray)	cpiwcgB384
CSPI05G28510	Watermelon (Charleston Gray)	cpiwcgB356
CSPI05G28510	Watermelon (Charleston Gray)	cpiwcgB360
CSPI05G28510	Watermelon (97103) v1	cpiwmB370
CSPI05G28510	Watermelon (97103) v1	cpiwmB432
CSPI05G28510	Cucurbita pepo (Zucchini)	cpecpiB177
CSPI05G28510	Cucurbita pepo (Zucchini)	cpecpiB414
CSPI05G28510	Bottle gourd (USVL1VR-Ls)	cpilsiB355
CSPI05G28510	Bottle gourd (USVL1VR-Ls)	cpilsiB352
CSPI05G28510	Melon (DHL92) v3.6.1	cpimedB339
CSPI05G28510	Melon (DHL92) v3.6.1	cpimedB377
CSPI05G28510	Cucumber (Gy14) v2	cgybcpiB035
CSPI05G28510	Cucumber (Gy14) v2	cgybcpiB225
CSPI05G28510	Silver-seed gourd	carcpiB0722
CSPI05G28510	Cucumber (Chinese Long) v3	cpicucB259
CSPI05G28510	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G28510	Watermelon (97103) v2	cpiwmbB357
CSPI05G28510	Watermelon (97103) v2	cpiwmbB429