Csa7G343870 (gene) Cucumber (Chinese Long) v2

NameCsa7G343870
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionPectinesterase; contains IPR006501 (Pectinesterase inhibitor), IPR011050 (Pectin lyase fold/virulence factor)
LocationChr7 : 12495508 .. 12499808 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTTTCTTTCTTCACAACTCTTCTCATTTCCAAACTATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTTTGCAAATTCATCTACCATCTTGATTATCTTGCTCATTTTGAAGTTAATAATGTATTGTGTACGTATACCTTTTTATTTTTGGTTCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGCACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGTATAATAGTGTTTTCAACTTTATTTCAAACCCTTTTATTTTGCTTTTATAAATATTTTTACTCCTACTCGTGCATTTTCAATCCTTTTAACTTTCTCAACAGAATATCCTGGTGCAGAAGCAGAACTACTGATTCCAATCACTTTTTTAGCTATGTTGTTTGGGTTCACAACTATAGTTTGTTAACTTAATTACTTACCATGAACATCTGTTGTTTTCATAAATTTATTGTGTTTTCATAACTCTACGATCATTTTCTTCTAGTATCTTGTTAAAAAAAATTACCTCTAAATCATCTTTAAAAGGTTTAATAAGTCTTTAAACTATCCATTAATTTTGTGTATAAGTTGGTCAACTTTCAAGTTTACATATAATATAGGGATTTTGAAATTTTCAAAATTTCCAAAAATTAGTTGTTCTTCTTAAATATAATTTTCTATATTGGAACCCTATAGTATAAAAATTAATGATATATCAAATAAAATTTTAAAGTATACACTTTTTCATAATTTAAATGTAATGAAAAAATCGTTAGTATATTAGGTATAACCAAATCTAAACGTCAAATAGTACCAAAATTATCTTTAAAAAAAATTGTATAGATTTGGCTAGCTACCCAAATTTAAACAATCAAATCTAAACAATCATGTACCAAATCTAAACGATCGTGTATGAAAAATCTTTGAAAAAGACTTGTTTACATTTAGTGAAATCTAAATGATCGTGTACTAAAAAATCTTGAAAAGAACTCATTTACATTTGGTGAAATCTAAATGACCGTGTACTAAATTACAATACCTAAATCTAACAAATCTAAACGATCGTGTACTAAAGAATGTTGAAAAAAATTGTTTAAATTTGGTCAAAATCTAAACAATCAAATCTAAACGATCATCTACCAAAGAAACAACCAATTCTAAACGATAGTGTACAAAAAAATCTTAAAAAATAATATTTAGATTTGGAACGATTGTTTACCAAATATACTGCCAATTAACAACGCATACATTTTTTTATATTTTTTATTATTGGTTTGTGAGTTTTTTCCATTTTCGAAATTGTTCTATGTACACCGTAATTATTTTGGTCTTTTGTTATATTTTTTAAAAGATTTCTAATATTTTAAAATGCTCATTTAGTTATCTAAGAACTGGAGTACCAGGACTAAAGAATTAATCTTGCCAAAAATATAATATACGATTTTTCTAACTTGAAATATGAAAGCAAACTCTTTTAGTTATAATTAATGTAAATAAGTGGAGAACTACAAAATTAATTTTGCCAAAAATCAGTATTCTATATTTGTAGAAATATGACAGCGAAAATTTGTAGTAATAACTAGTGTACTTTGATTCCTATCTTGGCTCTAAATAGGCTTTGTAGTGAACAAATCCAACACGTTTCTAGATCCAATCATAGACCATAACTTCTTCCATGTAGTTTAGTTTTTTTCAATAAGATTCAACCTCTGATCTCAAACGAGTAGGTGTGAGATATATTGATCTATTACTAGTGCAGAAAACAAAAAAGAAAGTATTATATCGATATTATTATTTAAGTTCATGTTGATATCAACAATATGTAGAATATAATTAACAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGCAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATATCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAAATCCGCCACACTTGGTAACACAAACTAAACCACAATACTCTTTTCTTTTCTTTTCTTTTTGTTTGAATTGTAGCTCCATTTTCTTCTTTTTAATTTAAGTGTTTTCTCCAAAAATAAATATAATAAAATAAACTAAAATTTCTTTTTGTTGATTTTAAGATGGACCATCTACCCTATTTTTATTCATTCAGCATAATTTTCTATTTTAAAAAATATATCTTCATTTCATGGAANNNNNNNNNNNNNNNNNNNNNNNTATTTGAGGAATAATAATTAAGAATATAGCAATATTTTAAAAAAATTGTAAATAAAGCAAAACTATCGTTGTTAGACTTGTATCATTGATAGAATTTGACCAATTTCGTTATATTTATAAATTTTTTAAAATTTTGTTATATATTTAATTATTTCTAATCTAATTGCTAAATTTGCAACTATCTCATTTGTTTTTCCTTTTTTTTCATATTTTTTTTGCCGAAGTTGGTCATTCAAAACTTAAAATTGGTAAAGGAAAAACTTGAATGATTTAGGAAAAACTACATATTGAATGATCATATGGTAAAATTTCTTTTTGTTGATTTTAAGATATATATTTTACGTATGTTCTCTAAAAAAAATGTTTTATTTTCTTAATTTCGTTGCATTATATTTGGACATTTATAAGGAATTATAGATCGCAAAAATGCAACAAAAAGAAAAACTACATATTGAATGAAATTTTTGCTATATCGTATTCTAATTATTTATCAAACCATTGGTCATTACATTAAAAAAAATACCCACATTTTAATAACAATGCTGTTTTTCTGTGACAGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTAATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGATCTAATGCGCCTAATTAATTAGCTTGCAACTCTTGCTTTTGGTTT

mRNA sequence

ATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGCACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGCAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATATCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAAATCCGCCACACTTGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTAATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGA

Coding sequence (CDS)

ATGGAAAACAATAAACTTTCACTCGCTCTCTTAGCTATTTTACTCCTCACAAATGCTATTGTTGTTAGAGCTAATTCTAATGAAAATAGTGTCATAGAAACCTCATGTGCCATTACTCTTTATCCTCAACTCTGTCATTCAACAATATCCTCCATCGTTGGAACGTCTAATTTGTTGAGTTTGAAAGATATTTTTGAAGTGTCATTGAGTGTTGCTATGGATGCAGCTAAGCATAACAATAAAAACATAAAAAAACTTATGGTGTCCACTAACAATGTTAGTAAACGAGACAAGATTGGTCTTCATGATTGTGTTGAAACAACTGACAGGACCATTTATGAGCTTGGGAAAGCTATAGAGGTCTTTCGTGAGTATCCGAACAAGAGATCTCTTACTTTGTACGCCGATGACCTCAAAACCTTCCTCAGTTCTGCCATTACTAATCAGGTGACTTGTTTGGATGGATTATCACATGACAAAACAGAGAAAAGGGTTTTGAGACTTATTGAGAACGCACATATTCATGTGACAAAGTTGTGTAGTAATGCATTGGCTTTGGTTCAAAAATTGACAACAGACATTGCCATAACAGATGAGAAAAGCTTGGTGGTCCATGATTTCCCTTATAAGATAACTTCAATACCCTCCCAAATGGATGACCCCAAAATTGTACTCTTTTCCAACCAGGAGGAAGATGAGAATAGAAGAAGGGAGGAGTTAGAAGATGGAATAAAGTGGCCAAAGTGGATGTCAATCGAAGACCAGAAGCTTTTGGAATCATCATCAGAAGCAGCAGCGGAGGCTGTGGTGGCGGCGGATGGAAGTGGAAATTATAAAACGGTGGCGGAGGCGGTGGCGGCAGCTCCGAGTAAGAACAGCAAAAGATATATAATAAAAATAAAGGCTGGAGAGTACTGGGAAAATGTGGATGTACCAAGCAGTAAAAGGAATATTATGTTTTGGGGAGATGGAAGATCCAACACCAAAATCATATCTAATAGAAGTCATGGAACTGGCTGGTCAACCTTCAAATCCGCCACACTTGCTGCGGTGGGGGATGGTTTTTTAGCTCGAGACATAACCTTTCAAAACAAAGCCGGAGCAGCCAACGGCCAAGCTGTAGCTCTCCGTGTTGGCTCAGATCACTCGGCCTTCTACCGATGTAGCATGCTTGCCTACCAAGACACTCTCTACGTTCACTCGAACCGTCAATTCTTCGTCAACTGCATTGTAGCAGGCACAGTCGATTTCATCTTTGGAAATGCAGCAGCCGTATTCCAAAACTCTGACATCACCCCTCGCAAACCAGGGCCTAGTCAAAGGAACATGGTCACAGCACAATCAAGAACCGACATTAATCAAAACACGGGTATCGTCATCCAAAAGTGTAGGATAAAAGCAACGTCAGACTTGGAGCCTGTGATAGAAGAATTCCCAAGCTTTCTCGGGAGACCATGGGAAGAATATGCTAGAGTTGTGGTAATGCAAACGACAATAAGCAATGTGATAGACAAAGAGGGGTGGTCCACATGGAATGGCCAAAGAAAGTCACCATACTATGCGGAATATGATAATAATGGAGCTGGAGCCGATATTTCTGGTAGAGTGCCATGGTCTCTTGTTATTGATGAGGCTCAAGCTAAGACTTTTACGGCTGGACCATTTATTGGTGGAGCTGATTGGCTTTCATCTACTGGCTTTCCCTATCAGCTTAGTCTCTATAAATGA

Protein sequence

MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK*
BLAST of Csa7G343870 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.5e-159
Identity = 307/592 (51.86%), Postives = 393/592 (66.39%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN------SVIETSCAITLYPQLCHST 61
           +  KL LAL A LL+  A++        R NS +N      +++++SC+ T YP LC S 
Sbjct: 26  KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSA 85

Query: 62  ISSIVGTSN-LLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           I+++   S  + S KD+ E+SL++   A +HN   I+KL+  TN ++KR+K+ LHDC+ET
Sbjct: 86  IAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTN-LTKREKVALHDCLET 145

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL KA+E   EYPNK+SL+ +ADDLKT +S+A+TNQ TCLDG SHD   K V  
Sbjct: 146 IDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRD 205

Query: 182 LIENAHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLF 241
            + +  +HV K+CSNALA+++ +T TD+ I    +        K+T   S +D       
Sbjct: 206 ALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN------NRKLTEETSTVDG------ 265

Query: 242 SNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVA 301
                              WP W+S  D++LL+SSS     AVVAADGSGN+KTVA AVA
Sbjct: 266 -------------------WPAWLSPGDRRLLQSSS-VTPNAVVAADGSGNFKTVAAAVA 325

Query: 302 AAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSA 361
           AAP   +KRYII+IKAG Y ENV+V    +NIMF GDGR+ T I  +R+   G +TFKSA
Sbjct: 326 AAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSA 385

Query: 362 TLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFF 421
           T A VG+GFLARDITFQN AG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFF
Sbjct: 386 TAAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFF 445

Query: 422 VNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIK 481
           VNC++AGTVDFIFGNAAAV QN DI  RKP   Q+NMVTAQ RTD NQNTGIVIQK RI 
Sbjct: 446 VNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIG 505

Query: 482 ATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYD 541
           ATSDL+PV   FP++LGRPW+EY+R V+MQ++I+++I   GW  W+G     + +Y E+ 
Sbjct: 506 ATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQ 565

Query: 542 NNGAGADISGRVPWS---LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           N+GAGA  SGRV W    ++    +A+ FT G FI G+ WL STGFP+ L L
Sbjct: 566 NSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Csa7G343870 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.2e-158
Identity = 306/592 (51.69%), Postives = 390/592 (65.88%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN------SVIETSCAITLYPQLCHST 61
           +  KL LAL A LL+  A++        R NS +N      +++++SC+ T YP LC S 
Sbjct: 26  KKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSA 85

Query: 62  ISSIVGTSN-LLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           I+++   S  + S KD+ E+SL++   A +HN   I+KL+  TN ++KR+K+ LHDC+ET
Sbjct: 86  IAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTN-LTKREKVALHDCLET 145

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL KA+E   EYPNK+SL+ +ADDLKT +S+A+TNQ TCLDG SHD   K V  
Sbjct: 146 IDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANKHVRD 205

Query: 182 LIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
            + +  +HV K+CSNALA+++ +T                            D  I+  S
Sbjct: 206 ALSDGQVHVEKMCSNALAMIKNMTDT--------------------------DMMIMRTS 265

Query: 242 NQEEDENRRR-EELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVA 301
           N     NR+  EE      WP W+S  D++LL+SSS      VVAADGSGN+KTVA +VA
Sbjct: 266 N-----NRKLIEETSTVDGWPAWLSTGDRRLLQSSS-VTPNVVVAADGSGNFKTVAASVA 325

Query: 302 AAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSA 361
           AAP   +KRYII+IKAG Y ENV+V    +NIMF GDGR+ T I  +R+   G +TFKSA
Sbjct: 326 AAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSA 385

Query: 362 TLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFF 421
           T+A VG+GFLARDITFQN AG +  QAVALRVG+D SAFY C MLAYQDTLYVHSNRQFF
Sbjct: 386 TVAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFF 445

Query: 422 VNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIK 481
           VNC++AGTVDFIFGNAAAV QN DI  RKP   Q+NMVTAQ R D NQNTGIVIQK RI 
Sbjct: 446 VNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIG 505

Query: 482 ATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYD 541
           ATSDL+PV   FP++LGRPW+EY+R V+MQ++I++VI   GW  W+G     + +Y E+ 
Sbjct: 506 ATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQ 565

Query: 542 NNGAGADISGRVPWS---LVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           N GAGA  SGRV W    ++    +A+ FT G FI G+ WL STGFP+ L L
Sbjct: 566 NAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Csa7G343870 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 291/562 (51.78%), Postives = 385/562 (68.51%), Query Frame = 1

Query: 18  NAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSN-LLSLKDIFEVSLSVAMDAA 77
           +A ++  +S+ +++++++C+ TL+P+LC+S I ++   S  + S KD+ E+SL++ + A 
Sbjct: 52  HADIMAISSSAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAV 111

Query: 78  KHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYAD 137
           + N   +K+L+ +   ++ R+K+ LHDC+ET D T+ EL  A+E    YPNK+SL  + +
Sbjct: 112 RRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVE 171

Query: 138 DLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQKLTTDIAI 197
           DLKT +SSAITNQ TCLDG SHD+ +K+V +++     HV K+CSNALA++  +T D  I
Sbjct: 172 DLKTLISSAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMT-DTDI 231

Query: 198 TDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQK 257
            +E  L          S P+                    R+ +ED  +WP+W+S  D++
Sbjct: 232 ANEMKL----------SAPAN------------------NRKLVEDNGEWPEWLSAGDRR 291

Query: 258 LLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKR 317
           LL+SS+    + VVAADGSG+YKTV+EAV  AP K+SKRY+I+IKAG Y ENVDVP  K 
Sbjct: 292 LLQSST-VTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKT 351

Query: 318 NIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGAANGQAVAL 377
           NIMF GDG+SNT I ++R+   G +TF SAT+  V    LARDITFQN AGA+  QAVAL
Sbjct: 352 NIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVAL 411

Query: 378 RVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKP 437
            VGSD SAFYRC MLAYQDTLYVHSNRQFFV C+VAGTVDFIFGN AAVFQ+ DI  R+P
Sbjct: 412 CVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRP 471

Query: 438 GPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQ 497
           G  Q+NMVTAQ RTD NQNTGIVIQKCRI ATSDL PV + FP++LGRPW+EY+R V+MQ
Sbjct: 472 GSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQ 531

Query: 498 TTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFT 557
           ++I++VI   GW  WNG     + +Y EY N GAGA  SGRV W    ++    +A+ +T
Sbjct: 532 SSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYT 583

Query: 558 AGPFIGGADWLSSTGFPYQLSL 574
            G FI G  WLSSTGFP+ L L
Sbjct: 592 PGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Csa7G343870 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 545.8 bits (1405), Expect = 5.6e-154
Identity = 304/594 (51.18%), Postives = 389/594 (65.49%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIVV-------RANS------NENSVIETSCAITLYPQLCHST 61
           +N KL L   A+ LL  A V        +AN       + ++V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETT 121
           + +  G   L S KD+ E S+++ + A +HN   +KKL+     ++ R+K  LHDC+ET 
Sbjct: 76  VVTAGGVE-LTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRL 181
           D T+ EL + +E    YP K++L  +A DLKT +SSAITNQ TCLDG SHD  +K+V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENAHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           +    IHV  +CSNALA+++ +T TDIA  ++K+        KITS   ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------E 255

Query: 242 NQEED---ENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEA 301
           NQE     +     EL D   WP W+S  D++LL+ S    A+A VAADGSG +KTVA A
Sbjct: 256 NQETTVAVDIAGAGEL-DSEGWPTWLSAGDRRLLQGSG-VKADATVAADGSGTFKTVAAA 315

Query: 302 VAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFK 361
           VAAAP  ++KRY+I IKAG Y ENV+V   K+NIMF GDGR+ T I  +R+   G +TF 
Sbjct: 316 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 375

Query: 362 SATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQ 421
           SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQ
Sbjct: 376 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 435

Query: 422 FFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCR 481
           FFV C++AGTVDFIFGNAA V Q+ DI  R+P   Q+NMVTAQ RTD NQNTGIVIQKCR
Sbjct: 436 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 495

Query: 482 IKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNG--QRKSPYYAE 541
           I ATSDL+ V   FP++LGRPW+EY++ V+MQ+ IS+VI  EGWS W G     +  Y E
Sbjct: 496 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 555

Query: 542 YDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           Y N GAGA  + RV W    ++   A+A+ +TAG FIGG  WLSSTGFP+ L L
Sbjct: 556 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Csa7G343870 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 538.5 bits (1386), Expect = 9.0e-152
Identity = 294/598 (49.16%), Postives = 388/598 (64.88%), Query Frame = 1

Query: 2   ENNK---LSLALLAILLLTNAIVVRA---NSNEN-----------SVIETSCAITLYPQL 61
           +NNK   LS A +A+LLL + + + A   N N+N           +++++ C+ TLYP+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 62  CHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDC 121
           C S +++  G   L S K++ E SL++   A KHN   +KKL+     ++ R+   LHDC
Sbjct: 75  CFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 134

Query: 122 VETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKR 181
           +ET D T+ EL  A+E   +YP ++SL  +ADDLKT +SSAITNQ TCLDG S+D  +++
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 182 VLRLIENAHIHVTKLCSNALALVQKLT-TDIA---ITDEKSLVVHDFPYKITSIPSQMDD 241
           V + +    +HV  +CSNALA+++ +T TDIA   + D+ S   ++   K+  +   +D 
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 254

Query: 242 PKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKT 301
                                DG  WPKW+S+ D++LL+ S+   A+A VA DGSG++ T
Sbjct: 255 ---------------------DG--WPKWLSVGDRRLLQGST-IKADATVADDGSGDFTT 314

Query: 302 VAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGW 361
           VA AVAAAP K++KR++I IKAG Y ENV+V   K NIMF GDGR  T I  +R+   G 
Sbjct: 315 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 374

Query: 362 STFKSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVH 421
           +TF SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY+C M AYQDTLYVH
Sbjct: 375 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 434

Query: 422 SNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVI 481
           SNRQFFV C + GTVDFIFGNAAAV Q+ DI  R+P   Q+NMVTAQ R+D NQNTGIVI
Sbjct: 435 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 494

Query: 482 QKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSP 541
           Q CRI  TSDL  V   FP++LGRPW+EY+R V+MQ+ IS+VI  EGW  W+G     + 
Sbjct: 495 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 554

Query: 542 YYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            Y EY N G GA  + RV W    ++  + +A+ FTAG FIGG  WL+STGFP+ LSL
Sbjct: 555 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Csa7G343870 vs. TrEMBL
Match: A0A0A0K9Y3_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G343870 PE=4 SV=1)

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60
           MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120
           LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180
           VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240
           SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300
           EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI
Sbjct: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300

Query: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360
           KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI
Sbjct: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360

Query: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420
           TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG
Sbjct: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420

Query: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480
           NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS
Sbjct: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480

Query: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540
           FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL
Sbjct: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540

Query: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 576
           VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK
Sbjct: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575

BLAST of Csa7G343870 vs. TrEMBL
Match: A0A0A0K6S6_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_7G343850 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 4.2e-281
Identity = 491/581 (84.51%), Postives = 529/581 (91.05%), Query Frame = 1

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       SVIETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI E+SLSVAMDAAK NN+NIKKL +S N V KRD+I LHDCV+TTDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAH 180
           EL KAI+ F EYPNK+SL  YADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN H
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
             VTKLCSNALALV+KLTTD+A+TDEKSLVVHDFPYKITSI SQ+DDPKIVLFSNQE+ E
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNS 300
           +RRRE+LE+GIKWP+WMSI+DQ LL+SSSE AA+ VVAADG+GNYKTV+EAV AAPSKNS
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAPSKNS 300

Query: 301 KRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGD 360
            RYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I ++RSHG+GWSTF SAT+ AVGD
Sbjct: 301 -RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVGD 360

Query: 361 GFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAG 420
           GFLARDITFQN AG+ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV C+VAG
Sbjct: 361 GFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVAG 420

Query: 421 TVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEP 480
           TVDFIFGNAAAV QNSD+TPRKPGP+Q NMVTAQSRTD+NQNTGIVIQKCRIKATSDLEP
Sbjct: 421 TVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 480

Query: 481 VIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADIS 540
           VI+EFPSFLGRPWEEYARVVVMQT+I+NVIDKEGWSTWNG  K PY+AEYDNNGAGAD S
Sbjct: 481 VIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADTS 540

Query: 541 GRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 575
           GRV WSLVI+EA+AKTFTA PFI GA WL STGFPYQL LY
Sbjct: 541 GRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLY 580

BLAST of Csa7G343870 vs. TrEMBL
Match: A0A0A0LCR6_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G646640 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 1.1e-172
Identity = 320/570 (56.14%), Postives = 408/570 (71.58%), Query Frame = 1

Query: 10  LLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSL 69
           LL   L T  ++   +S+++  + + CA+TLYP+LC +TIS+ VG+S+    K+  E S+
Sbjct: 22  LLVFALSTTLLLAVVSSDDH--VGSKCAMTLYPELCETTISTAVGSSS----KEAIEASV 81

Query: 70  SVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKR 129
           ++ + A K N K ++KL+ +  N++KR KI  HDC+ET + T+ EL + +E   EYP K+
Sbjct: 82  NITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKK 141

Query: 130 SLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQK 189
           SL+ YADDLKT LSS ITNQ TC+DG SHDK +K+V   ++   IH+ KLCS ALAL++ 
Sbjct: 142 SLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKN 201

Query: 190 LT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPK 249
           LT TDIA  +  +   H                      N+++ E ++   +EDGIKWP 
Sbjct: 202 LTDTDIANLNNNNNNNH---------------------LNRKQLEEKK---MEDGIKWPD 261

Query: 250 WMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWEN 309
           WMS +D++LL++SS A  + VVAADGSG+++T++EAVAAAPS++S+RYII+IKAG Y EN
Sbjct: 262 WMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYREN 321

Query: 310 VDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGA 369
           V+V SSKRNIMFWGDGR NT I  NR+   G +TF SAT+AAVG+ FLARD+TFQN AG 
Sbjct: 322 VNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGP 381

Query: 370 ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQN 429
           +  QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQF+V CI+ GT+DFIFGNAAAV Q+
Sbjct: 382 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQD 441

Query: 430 SDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEE 489
            DI  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL PVI  FP+FLGRPW+ 
Sbjct: 442 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 501

Query: 490 YARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAGADISGRVPWS---LVID 549
           Y+R VVMQT+ISNVID  GW  W+G     + +YAEY N+GAGAD S RV W    ++  
Sbjct: 502 YSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTR 561

Query: 550 EAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            A+A+ FTAG FIGG  WLSSTGFP+ L L
Sbjct: 562 AAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of Csa7G343870 vs. TrEMBL
Match: H6WYP0_GOSHI (Pectinesterase OS=Gossypium hirsutum GN=PME2 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 7.1e-164
Identity = 315/591 (53.30%), Postives = 409/591 (69.20%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN-----SVIETSCAITLYPQLCHSTI 61
           ++ K+ LAL A L++  AI+        R NS+E+     +V++++C+ T YP LC S +
Sbjct: 23  KHKKIFLALFASLVIVAAIIGIVAGVSSRNNSDESDTSHHAVVKSACSGTFYPDLCFSAV 82

Query: 62  SSI-VGTSNLL-SLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           +++  GT+  + S KD+ E+SL++   A +HN   IKKL+ +  +++ R+K  LHDC+ET
Sbjct: 83  TTVPAGTAKKVRSQKDVIELSLNITTTAVEHNYFKIKKLL-ARKDLTTREKTALHDCLET 142

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL +A+E   EYPNK+SLT +ADDLKT +S+A+TNQ TCLDG SH+  +K++  
Sbjct: 143 IDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLDGFSHEGADKKIRE 202

Query: 182 LIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           ++ +   +V K+CSNALA+++ +T D  I +E  L                         
Sbjct: 203 VLIDGEKYVEKMCSNALAMIKNMT-DTDIANEMML------------------------- 262

Query: 242 NQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAA 301
              +  NR+ +E E GI WP+W+S  D++LL+SSS      VVAADGSGN+KTV+EAVA 
Sbjct: 263 ---KSSNRKLKEDESGIAWPEWLSAGDRRLLQSSS-VTPNVVVAADGSGNFKTVSEAVAK 322

Query: 302 APSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSAT 361
           AP K+SKRYII+IKAG Y ENV+VP  K NIMF GDGR+ T I  +R+   G +TF SAT
Sbjct: 323 APEKSSKRYIIRIKAGVYRENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSAT 382

Query: 362 LAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV 421
           +AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQF+V
Sbjct: 383 VAAVGEKFLARDITFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYV 442

Query: 422 NCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKA 481
           NC+VAGTVDFIFGNAAAVFQN DI  RKP   Q+NMVTAQ RTD NQNTGIVIQKCRI A
Sbjct: 443 NCLVAGTVDFIFGNAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 502

Query: 482 TSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDN 541
           TSDL+PV + FP++LGRPW+EY+R VVMQ+TIS+VI   GW  W+G    K+ +YAEY N
Sbjct: 503 TSDLQPVRKNFPTYLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQN 562

Query: 542 NGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            GAGA  S RV W    ++   ++A+ FT G FI G  WLSSTGFP+ L L
Sbjct: 563 TGAGASTSARVKWGGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582

BLAST of Csa7G343870 vs. TrEMBL
Match: A0A0B0NC33_GOSAR (Pectinesterase OS=Gossypium arboreum GN=F383_15549 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 9.3e-164
Identity = 314/591 (53.13%), Postives = 409/591 (69.20%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIV-------VRANSNEN-----SVIETSCAITLYPQLCHSTI 61
           ++ K+ LAL A L++  AI+        R NS+E+     ++++++C+ T YP LC S +
Sbjct: 23  KHKKIFLALFASLVIVAAIIGIVAGVSSRNNSDESDTSHHAIVKSACSGTFYPDLCFSAV 82

Query: 62  SSI-VGTSNLL-SLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVET 121
           +++  GT+  + S KD+ E+SL++   A +HN   IKKL+ +  +++ R+K  LHDC+ET
Sbjct: 83  ATVPAGTAKKVRSQKDVIELSLNITTTAVEHNYFKIKKLL-ARKDLTTREKTALHDCLET 142

Query: 122 TDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLR 181
            D T+ EL +A+E   EYPNK+SLT +ADDLKT +S+A+TNQ TCLDG SH+  +K++  
Sbjct: 143 IDETLDELHEAVEDLHEYPNKKSLTQHADDLKTLMSAAMTNQETCLDGFSHEGADKKIRE 202

Query: 182 LIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           ++ +   +V K+CSNALA+++ +T D  I +E  L                         
Sbjct: 203 VLIDGEKYVEKMCSNALAMIKNMT-DTDIANEMML------------------------- 262

Query: 242 NQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAA 301
              +  NR+ +E E GI WP+W+S  D++LL+SSS      VVAADGSGN+KTV+EAVA 
Sbjct: 263 ---KSSNRKLKEDESGIAWPEWLSAGDRRLLQSSS-VTPNVVVAADGSGNFKTVSEAVAK 322

Query: 302 APSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSAT 361
           AP K+SKRYII+IKAG Y ENV+VP  K NIMF GDGR+ T I  +R+   G +TF SAT
Sbjct: 323 APEKSSKRYIIRIKAGVYIENVEVPKKKSNIMFIGDGRTKTIITGSRNVVDGSTTFHSAT 382

Query: 362 LAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV 421
           +AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQF+V
Sbjct: 383 VAAVGEKFLARDITFQNTAGPSKHQAVALRVGSDLSAFYNCDMLAYQDTLYVHSNRQFYV 442

Query: 422 NCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKA 481
           NC+VAGTVDFIFGNAAAVFQN DI  RKP   Q+NMVTAQ RTD NQNTGIVIQKCRI A
Sbjct: 443 NCLVAGTVDFIFGNAAAVFQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 502

Query: 482 TSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDN 541
           TSDL+PV + FP++LGRPW+EY+R VVMQ+TIS+VI   GW  W+G    K+ +YAEY N
Sbjct: 503 TSDLQPVRKNFPTYLGRPWKEYSRTVVMQSTISDVIQPAGWHEWSGSFALKTLFYAEYQN 562

Query: 542 NGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            GAGA  S RV W    ++   ++A+ FT G FI G  WLSSTGFP+ L L
Sbjct: 563 TGAGASTSARVKWGGYKVITSASEAQAFTPGRFIAGGSWLSSTGFPFALGL 582

BLAST of Csa7G343870 vs. TAIR10
Match: AT3G14310.1 (AT3G14310.1 pectin methylesterase 3)

HSP 1 Score: 545.8 bits (1405), Expect = 3.2e-155
Identity = 304/594 (51.18%), Postives = 389/594 (65.49%), Query Frame = 1

Query: 2   ENNKLSLALLAILLLTNAIVV-------RANS------NENSVIETSCAITLYPQLCHST 61
           +N KL L   A+ LL  A V        +AN       + ++V+ +SC+ T YP+LC S 
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCISA 75

Query: 62  ISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETT 121
           + +  G   L S KD+ E S+++ + A +HN   +KKL+     ++ R+K  LHDC+ET 
Sbjct: 76  VVTAGGVE-LTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 DRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRL 181
           D T+ EL + +E    YP K++L  +A DLKT +SSAITNQ TCLDG SHD  +K+V + 
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 IENAHIHVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFS 241
           +    IHV  +CSNALA+++ +T TDIA  ++K+        KITS   ++ +       
Sbjct: 196 LLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKA--------KITSNNRKLKE------E 255

Query: 242 NQEED---ENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEA 301
           NQE     +     EL D   WP W+S  D++LL+ S    A+A VAADGSG +KTVA A
Sbjct: 256 NQETTVAVDIAGAGEL-DSEGWPTWLSAGDRRLLQGSG-VKADATVAADGSGTFKTVAAA 315

Query: 302 VAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFK 361
           VAAAP  ++KRY+I IKAG Y ENV+V   K+NIMF GDGR+ T I  +R+   G +TF 
Sbjct: 316 VAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFH 375

Query: 362 SATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQ 421
           SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY C MLAYQDTLYVHSNRQ
Sbjct: 376 SATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQ 435

Query: 422 FFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCR 481
           FFV C++AGTVDFIFGNAA V Q+ DI  R+P   Q+NMVTAQ RTD NQNTGIVIQKCR
Sbjct: 436 FFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCR 495

Query: 482 IKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNG--QRKSPYYAE 541
           I ATSDL+ V   FP++LGRPW+EY++ V+MQ+ IS+VI  EGWS W G     +  Y E
Sbjct: 496 IGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYRE 555

Query: 542 YDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           Y N GAGA  + RV W    ++   A+A+ +TAG FIGG  WLSSTGFP+ L L
Sbjct: 556 YSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Csa7G343870 vs. TAIR10
Match: AT1G53830.1 (AT1G53830.1 pectin methylesterase 2)

HSP 1 Score: 538.5 bits (1386), Expect = 5.0e-153
Identity = 294/598 (49.16%), Postives = 388/598 (64.88%), Query Frame = 1

Query: 2   ENNK---LSLALLAILLLTNAIVVRA---NSNEN-----------SVIETSCAITLYPQL 61
           +NNK   LS A +A+LLL + + + A   N N+N           +++++ C+ TLYP+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 62  CHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDC 121
           C S +++  G   L S K++ E SL++   A KHN   +KKL+     ++ R+   LHDC
Sbjct: 75  CFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDC 134

Query: 122 VETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKR 181
           +ET D T+ EL  A+E   +YP ++SL  +ADDLKT +SSAITNQ TCLDG S+D  +++
Sbjct: 135 LETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRK 194

Query: 182 VLRLIENAHIHVTKLCSNALALVQKLT-TDIA---ITDEKSLVVHDFPYKITSIPSQMDD 241
           V + +    +HV  +CSNALA+++ +T TDIA   + D+ S   ++   K+  +   +D 
Sbjct: 195 VRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDS 254

Query: 242 PKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKT 301
                                DG  WPKW+S+ D++LL+ S+   A+A VA DGSG++ T
Sbjct: 255 ---------------------DG--WPKWLSVGDRRLLQGST-IKADATVADDGSGDFTT 314

Query: 302 VAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGW 361
           VA AVAAAP K++KR++I IKAG Y ENV+V   K NIMF GDGR  T I  +R+   G 
Sbjct: 315 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 374

Query: 362 STFKSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVH 421
           +TF SAT+AAVG+ FLARDITFQN AG +  QAVALRVGSD SAFY+C M AYQDTLYVH
Sbjct: 375 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 434

Query: 422 SNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVI 481
           SNRQFFV C + GTVDFIFGNAAAV Q+ DI  R+P   Q+NMVTAQ R+D NQNTGIVI
Sbjct: 435 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 494

Query: 482 QKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSP 541
           Q CRI  TSDL  V   FP++LGRPW+EY+R V+MQ+ IS+VI  EGW  W+G     + 
Sbjct: 495 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 554

Query: 542 YYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            Y EY N G GA  + RV W    ++  + +A+ FTAG FIGG  WL+STGFP+ LSL
Sbjct: 555 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Csa7G343870 vs. TAIR10
Match: AT1G11580.1 (AT1G11580.1 methylesterase PCR A)

HSP 1 Score: 352.4 bits (903), Expect = 5.1e-97
Identity = 224/592 (37.84%), Postives = 322/592 (54.39%), Query Frame = 1

Query: 2   ENNKLSLALLAIL-----LLTNAIVVRANSNENSVIETSCAITLYPQLCHS-----TISS 61
           +N  L L+ +AIL          I V  N+N++S++ TS       Q+CH      +  +
Sbjct: 18  KNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTS-------QICHGAHDQDSCQA 77

Query: 62  IVGTSNLLSLK-----DIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVE 121
           ++     LSL      D+  V L  ++   +     + +  + +N V  RDK G  DC E
Sbjct: 78  LLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGV--RDKAGFADCEE 137

Query: 122 TTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVL 181
             D +   +  ++E  R      +L  Y++ + T+LSS +TN +TCL+ +S         
Sbjct: 138 MMDVSKDRMMSSMEELRG--GNYNLESYSN-VHTWLSSVLTNYMTCLESISD-------- 197

Query: 182 RLIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLF 241
                       +  N+  +V+    D+      +L +      ++ +P++ DD K+++ 
Sbjct: 198 ------------VSVNSKQIVKPQLEDLVSRARVALAIF-----VSVLPAR-DDLKMIIS 257

Query: 242 SNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEA---AAEAVVAADGSGNYKTVAE 301
           +                 ++P W++  D+KLLESS +     A  VVA DG+G +KTV E
Sbjct: 258 N-----------------RFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNE 317

Query: 302 AVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTF 361
           AVAAAP  ++ RY+I +K G Y E +D+   K+N+M  GDG+  T I  + +   G +TF
Sbjct: 318 AVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTF 377

Query: 362 KSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNR 421
           +SAT+AA GDGF+A+DI FQN AG A  QAVALRV +D +   RC + AYQDTLY H+ R
Sbjct: 378 RSATVAANGDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLR 437

Query: 422 QFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKC 481
           QF+ +  + GTVDFIFGN+A VFQN DI  R PG  Q+NM+TAQ R D NQNT I IQKC
Sbjct: 438 QFYRDSYITGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKC 497

Query: 482 RIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYA 541
           +I A+SDL PV     +FLGRPW+ Y+R V+MQ+ I N ID  GW  W+G+    + YY 
Sbjct: 498 KITASSDLAPVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYG 554

Query: 542 EYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQ 571
           EY N G GAD S RV W    ++ D  +A+ FT    I G  WL  TG  +Q
Sbjct: 558 EYANTGPGADTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQ 554

BLAST of Csa7G343870 vs. TAIR10
Match: AT2G45220.1 (AT2G45220.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 340.1 bits (871), Expect = 2.6e-93
Identity = 211/539 (39.15%), Postives = 299/539 (55.47%), Query Frame = 1

Query: 32  IETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTN 91
           ++  C+ T  P+ C   ++       + S  +  ++S+ + +D A     +   L     
Sbjct: 30  VKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCR 89

Query: 92  NVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVT 151
           +   R+K    DC++  D T+ ++ + ++     PN +   L   D +T+LS+A+TN  T
Sbjct: 90  DT--REKAAWEDCIKLYDLTVSKINETMD-----PNVKCSKL---DAQTWLSTALTNLDT 149

Query: 152 CLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKI 211
           C  G         VL L+ N   +V+ L  N L                          I
Sbjct: 150 CRAGFLELGVTDIVLPLMSN---NVSNLLCNTL-------------------------AI 209

Query: 212 TSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVA 271
             +P     P+                  +DG  +P W+   D+KLL+SS+     AVVA
Sbjct: 210 NKVPFNYTPPE------------------KDG--FPSWVKPGDRKLLQSSTPKD-NAVVA 269

Query: 272 ADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKII 331
            DGSGN+KT+ EA+ AA    S R++I +K G Y EN+++   K+N+M  GDG   T I 
Sbjct: 270 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIIT 329

Query: 332 SNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSML 391
            ++S G G +TF SAT+AAVGDGF+AR ITF+N AGA+N QAVALR GSD S FY+CS  
Sbjct: 330 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 389

Query: 392 AYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTD 451
           AYQDTLYVHSNRQF+ +C V GTVDFIFGNAAAV QN +I  R+P  S+ N +TAQ R+D
Sbjct: 390 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSD 449

Query: 452 INQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTW 511
            NQNTGI+I   R+ A SDL PV+    ++LGRPW +Y+R V M+T++ ++ID  GW  W
Sbjct: 450 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 504

Query: 512 NGQ--RKSPYYAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSST 566
           +G    K+ +YAE+ N G GA  SGRV W    ++   ++A  FT G F+ G  W+ S+
Sbjct: 510 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS 504

BLAST of Csa7G343870 vs. TAIR10
Match: AT4G02320.1 (AT4G02320.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 338.2 bits (866), Expect = 1.0e-92
Identity = 196/537 (36.50%), Postives = 292/537 (54.38%), Query Frame = 1

Query: 47  STISSIVGTSNLLSLKDIFEV---SLSVAMDAAKHNNKNIKKLMVST-NNVSKRDKIGLH 106
           ST+ S   T    S  DI E+   +L+  +     ++ N   L+    +N+S RD     
Sbjct: 20  STVFSSHVTQRFTSSDDITELVVTALNQTISNVNLSSSNFSDLLQRLGSNLSHRDLCAFD 79

Query: 107 DCVETTDRTIYELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTE 166
           DC+E  D T+++L  AI   R +  +        ++K  LS+A+TN  TCLDG +    +
Sbjct: 80  DCLELLDDTVFDLTTAISKLRSHSPE------LHNVKMLLSAAMTNTRTCLDGFASSDND 139

Query: 167 KRVLRLIENAHIHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPK 226
                  EN + +  K    A +L + L    +   +   ++ + P  I           
Sbjct: 140 -------ENLNNNDNKTYGVAESLKESLFNISSHVSDSLAMLENIPGHIPG--------- 199

Query: 227 IVLFSNQEEDENRRREELEDGIKWPKWMSIEDQKLLESS-SEAAAEAVVAADGSGNYKTV 286
                           ++++ + +P W+S  D+ LL+    E     VVA +G+GNY T+
Sbjct: 200 ----------------KVKEDVGFPMWVSGSDRNLLQDPVDETKVNLVVAQNGTGNYTTI 259

Query: 287 AEAVAAAPSKNSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWS 346
            EA++AAP+ +  R++I IK GEY+EN+++P  K  IMF GDG   T I +NRS+  GW+
Sbjct: 260 GEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANRSYADGWT 319

Query: 347 TFKSATLAAVGDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHS 406
            F SAT+   G GF+A+D++F N AG    QAVALR  SD SA+YRCS  +YQDT+YVHS
Sbjct: 320 AFHSATVGVRGSGFIAKDLSFVNYAGPEKHQAVALRSSSDLSAYYRCSFESYQDTIYVHS 379

Query: 407 NRQFFVNCIVAGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQ 466
           ++QF+  C + GTVDFIFG+A+ VFQN  +  R+P P+Q+ + TAQ R +  + TGI I 
Sbjct: 380 HKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRENSREPTGISII 439

Query: 467 KCRIKATSDLEPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPY 526
             RI A  DL PV   F ++LGRPW+ Y+R V+M++ I +++D  GW  W      ++ Y
Sbjct: 440 SSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLKWKDDFALETLY 499

Query: 527 YAEYDNNGAGADISGRVPW---SLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
           Y EY N G G++++ RV W     +    +A  F+ GPFI G  WL+ST  P+ L L
Sbjct: 500 YGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNSTRIPFTLDL 518

BLAST of Csa7G343870 vs. NCBI nr
Match: gi|778727001|ref|XP_004139620.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 1

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60
           MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS
Sbjct: 1   MENNKLSLALLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLS 60

Query: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120
           LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE
Sbjct: 61  LKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIE 120

Query: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180
           VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC
Sbjct: 121 VFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLC 180

Query: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240
           SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL
Sbjct: 181 SNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREEL 240

Query: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300
           EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI
Sbjct: 241 EDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKI 300

Query: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360
           KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI
Sbjct: 301 KAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDI 360

Query: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420
           TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG
Sbjct: 361 TFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFG 420

Query: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480
           NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS
Sbjct: 421 NAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPS 480

Query: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540
           FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL
Sbjct: 481 FLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADISGRVPWSL 540

Query: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 576
           VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK
Sbjct: 541 VIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLYK 575

BLAST of Csa7G343870 vs. NCBI nr
Match: gi|778726997|ref|XP_011659194.1| (PREDICTED: pectinesterase [Cucumis sativus])

HSP 1 Score: 974.9 bits (2519), Expect = 6.0e-281
Identity = 491/581 (84.51%), Postives = 529/581 (91.05%), Query Frame = 1

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       SVIETSCAITLYPQLCHSTISSI+
Sbjct: 1   MENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSIL 60

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI E+SLSVAMDAAK NN+NIKKL +S N V KRD+I LHDCV+TTDRTIY
Sbjct: 61  GTSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIY 120

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAH 180
           EL KAI+ F EYPNK+SL  YADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIEN H
Sbjct: 121 ELDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTH 180

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
             VTKLCSNALALV+KLTTD+A+TDEKSLVVHDFPYKITSI SQ+DDPKIVLFSNQE+ E
Sbjct: 181 NQVTKLCSNALALVKKLTTDVALTDEKSLVVHDFPYKITSISSQIDDPKIVLFSNQEDGE 240

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNS 300
           +RRRE+LE+GIKWP+WMSI+DQ LL+SSSE AA+ VVAADG+GNYKTV+EAV AAPSKNS
Sbjct: 241 SRRREDLENGIKWPEWMSIQDQMLLDSSSEEAADVVVAADGTGNYKTVSEAVKAAPSKNS 300

Query: 301 KRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGD 360
            RYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I ++RSHG+GWSTF SAT+ AVGD
Sbjct: 301 -RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAVGD 360

Query: 361 GFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAG 420
           GFLARDITFQN AG+ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFV C+VAG
Sbjct: 361 GFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVVAG 420

Query: 421 TVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEP 480
           TVDFIFGNAAAV QNSD+TPRKPGP+Q NMVTAQSRTD+NQNTGIVIQKCRIKATSDLEP
Sbjct: 421 TVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEP 480

Query: 481 VIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADIS 540
           VI+EFPSFLGRPWEEYARVVVMQT+I+NVIDKEGWSTWNG  K PY+AEYDNNGAGAD S
Sbjct: 481 VIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGDIKKPYFAEYDNNGAGADTS 540

Query: 541 GRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 575
           GRV WSLVI+EA+AKTFTA PFI GA WL STGFPYQL LY
Sbjct: 541 GRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGLY 580

BLAST of Csa7G343870 vs. NCBI nr
Match: gi|659116371|ref|XP_008458040.1| (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 964.9 bits (2493), Expect = 6.2e-278
Identity = 488/582 (83.85%), Postives = 526/582 (90.38%), Query Frame = 1

Query: 1   MENNKLSLALLAILLLTNAIVVRANSNEN-------SVIETSCAITLYPQLCHSTISSIV 60
           MEN KLSLAL A LLLTNAI+VRANSNEN       S+IETSCAITLYPQLCHSTISSI+
Sbjct: 23  MENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSII 82

Query: 61  GTSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIY 120
           GTSNLLSLKDI ++SLSVAMDAAK NN NIK LM S NNVSKRD+I LHDCV++TDRTIY
Sbjct: 83  GTSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNLM-SFNNVSKRDRIPLHDCVKSTDRTIY 142

Query: 121 ELGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAH 180
           EL KAIE FREYPNK+SLT YADDLKTFLSSAITNQVTCLDG   DKTEK VLR+IEN H
Sbjct: 143 ELDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTH 202

Query: 181 IHVTKLCSNALALVQKLTTDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDE 240
           IH TKLCSNALALV+KLTT+IA+ DEK+LVVHDFPYKITSI SQ++D KIVLFSNQEE E
Sbjct: 203 IHATKLCSNALALVKKLTTEIALIDEKNLVVHDFPYKITSISSQINDLKIVLFSNQEEGE 262

Query: 241 NRRREELEDGIKWPKWMSIEDQKLLESSS-EAAAEAVVAADGSGNYKTVAEAVAAAPSKN 300
           N RRE+LE+GIKWP+WMSI+DQKLLESSS EAAA+ VVA DGSGNYKTV+EAV AAPSKN
Sbjct: 263 NGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSKN 322

Query: 301 SKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVG 360
           SKRYIIKIKAG Y ENVDVPSSKRNIMFWGDGRSNT I + RSHG+GWSTF SAT+ AVG
Sbjct: 323 SKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVG 382

Query: 361 DGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVA 420
           DGFLARDI+F+N AG ANGQAVALRVGSDHSAFYRC ML YQDTLYVHSNRQFFVNC+VA
Sbjct: 383 DGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVA 442

Query: 421 GTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLE 480
           GTVDF+FGNAAAV QNSD+TPRKPGP+QRNMVTAQSRTD+NQNTGIVIQKCRIKATSDLE
Sbjct: 443 GTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLE 502

Query: 481 PVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQRKSPYYAEYDNNGAGADI 540
           PVI+EFPSFLGRPWEEYARVVVMQTTI+NVIDK+GWSTWNG  K PY+AEYDNNGAGA+ 
Sbjct: 503 PVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGDIKKPYFAEYDNNGAGANT 562

Query: 541 SGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSLY 575
           SGRVPWSLVI+EA AKTFTA  FIGGADWL STGFPYQL LY
Sbjct: 563 SGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGLY 603

BLAST of Csa7G343870 vs. NCBI nr
Match: gi|778682530|ref|XP_004144447.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 614.8 bits (1584), Expect = 1.6e-172
Identity = 320/570 (56.14%), Postives = 408/570 (71.58%), Query Frame = 1

Query: 10  LLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSL 69
           LL   L T  ++   +S+++  + + CA+TLYP+LC +TIS+ VG+S+    K+  E S+
Sbjct: 22  LLVFALSTTLLLAVVSSDDH--VGSKCAMTLYPELCETTISTAVGSSS----KEAIEASV 81

Query: 70  SVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKR 129
           ++ + A K N K ++KL+ +  N++KR KI  HDC+ET + T+ EL + +E   EYP K+
Sbjct: 82  NITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKK 141

Query: 130 SLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQK 189
           SL+ YADDLKT LSS ITNQ TC+DG SHDK +K+V   ++   IH+ KLCS ALAL++ 
Sbjct: 142 SLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKN 201

Query: 190 LT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPK 249
           LT TDIA  +  +   H                      N+++ E ++   +EDGIKWP 
Sbjct: 202 LTDTDIANLNNNNNNNH---------------------LNRKQLEEKK---MEDGIKWPD 261

Query: 250 WMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWEN 309
           WMS +D++LL++SS A  + VVAADGSG+++T++EAVAAAPS++S+RYII+IKAG Y EN
Sbjct: 262 WMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYREN 321

Query: 310 VDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGA 369
           V+V SSKRNIMFWGDGR NT I  NR+   G +TF SAT+AAVG+ FLARD+TFQN AG 
Sbjct: 322 VNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGP 381

Query: 370 ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQN 429
           +  QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQF+V CI+ GT+DFIFGNAAAV Q+
Sbjct: 382 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQD 441

Query: 430 SDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEE 489
            DI  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL PVI  FP+FLGRPW+ 
Sbjct: 442 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 501

Query: 490 YARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAGADISGRVPWS---LVID 549
           Y+R VVMQT+ISNVID  GW  W+G     + +YAEY N+GAGAD S RV W    ++  
Sbjct: 502 YSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTR 561

Query: 550 EAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            A+A+ FTAG FIGG  WLSSTGFP+ L L
Sbjct: 562 AAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of Csa7G343870 vs. NCBI nr
Match: gi|659120800|ref|XP_008460358.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 613.2 bits (1580), Expect = 4.6e-172
Identity = 318/570 (55.79%), Postives = 404/570 (70.88%), Query Frame = 1

Query: 10  LLAILLLTNAIVVRANSNENSVIETSCAITLYPQLCHSTISSIVGTSNLLSLKDIFEVSL 69
           LL   L T  ++   +S+++  + + CA+TLYP+LC +TIS+ VG+S+    K+  E S+
Sbjct: 22  LLVFALSTTLLLAAVSSDDH--VRSKCAMTLYPELCETTISTAVGSSS----KEAIEASV 81

Query: 70  SVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYELGKAIEVFREYPNKR 129
           ++ + A   N + +KKL+ + N+++KR+KI LHDC+ET + T+ EL + +E   EYPNK+
Sbjct: 82  NITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRELYEVVEDVNEYPNKK 141

Query: 130 SLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHIHVTKLCSNALALVQK 189
           SL+ YADDLKT LSSAITNQ TCLDG SHDK +K+V   ++   IH+ KLCS ALAL++ 
Sbjct: 142 SLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLIHIEKLCSIALALIKN 201

Query: 190 LT-TDIAITDEKSLVVHDFPYKITSIPSQMDDPKIVLFSNQEEDENRRREELEDGIKWPK 249
           LT TDIA  +  +                          N    +  + +  +D I WP 
Sbjct: 202 LTDTDIANLNNNN--------------------------NHLSRKQLKEKNTDDRINWPD 261

Query: 250 WMSIEDQKLLESSSEAAAEAVVAADGSGNYKTVAEAVAAAPSKNSKRYIIKIKAGEYWEN 309
           WMS  D++LL++SS A  + VVAADGSG+++T++EAVAAAPS++S+RYII+IKAG Y EN
Sbjct: 262 WMSPNDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYREN 321

Query: 310 VDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAVGDGFLARDITFQNKAGA 369
           V+V SSKRNIMFWGDGR NT I  NR+   G +TF SAT+AAVG+ FLARD+TFQN AG 
Sbjct: 322 VNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGP 381

Query: 370 ANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIVAGTVDFIFGNAAAVFQN 429
           +  QAVALRVGSD SAFYRC MLAYQDTLYVHSNRQF+V CI+ GT+DFIFGNAAAV Q+
Sbjct: 382 SKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQD 441

Query: 430 SDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDLEPVIEEFPSFLGRPWEE 489
            DI  R+P P QRNMVTAQ RTD NQNTGIVIQKCRI  TSDL PVI  FP+FLGRPW+ 
Sbjct: 442 CDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQR 501

Query: 490 YARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAGADISGRVPWS---LVID 549
           Y+R VVMQT+ISNVID  GW  W+G     + +YAEY N+GAGAD S RV W    ++  
Sbjct: 502 YSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTR 559

Query: 550 EAQAKTFTAGPFIGGADWLSSTGFPYQLSL 574
            A+A+ FTAG FIGG  WLSSTGFP+ L L
Sbjct: 562 AAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PME1_CITSI1.5e-15951.86Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1[more]
PME3_CITSI2.2e-15851.69Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
PMEU1_SOLLC1.1e-15751.78Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
PME3_ARATH5.6e-15451.18Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME2_ARATH9.0e-15249.16Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K9Y3_CUCSA0.0e+00100.00Pectinesterase OS=Cucumis sativus GN=Csa_7G343870 PE=4 SV=1[more]
A0A0A0K6S6_CUCSA4.2e-28184.51Pectinesterase OS=Cucumis sativus GN=Csa_7G343850 PE=4 SV=1[more]
A0A0A0LCR6_CUCSA1.1e-17256.14Pectinesterase OS=Cucumis sativus GN=Csa_3G646640 PE=4 SV=1[more]
H6WYP0_GOSHI7.1e-16453.30Pectinesterase OS=Gossypium hirsutum GN=PME2 PE=2 SV=1[more]
A0A0B0NC33_GOSAR9.3e-16453.13Pectinesterase OS=Gossypium arboreum GN=F383_15549 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.13.2e-15551.18 pectin methylesterase 3[more]
AT1G53830.15.0e-15349.16 pectin methylesterase 2[more]
AT1G11580.15.1e-9737.84 methylesterase PCR A[more]
AT2G45220.12.6e-9339.15 Plant invertase/pectin methylesterase inhibitor superfamily[more]
AT4G02320.11.0e-9236.50 Plant invertase/pectin methylesterase inhibitor superfamily[more]
Match NameE-valueIdentityDescription
gi|778727001|ref|XP_004139620.2|0.0e+00100.00PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|778726997|ref|XP_011659194.1|6.0e-28184.51PREDICTED: pectinesterase [Cucumis sativus][more]
gi|659116371|ref|XP_008458040.1|6.2e-27883.85PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
gi|778682530|ref|XP_004144447.2|1.6e-17256.14PREDICTED: pectinesterase-like [Cucumis sativus][more]
gi|659120800|ref|XP_008460358.1|4.6e-17255.79PREDICTED: pectinesterase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000070Pectinesterase_cat
IPR006501Pectinesterase_inhib_dom
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Cellular Component
TermDefinition
GO:0005618cell wall
Vocabulary: Molecular Function
TermDefinition
GO:0030599pectinesterase activity
GO:0004857enzyme inhibitor activity
Vocabulary: Biological Process
TermDefinition
GO:0042545cell wall modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005618 cell wall
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU095147cucumber EST collection version 3.0transcribed_cluster
CU124644cucumber EST collection version 3.0transcribed_cluster
CU140420cucumber EST collection version 3.0transcribed_cluster
CU148209cucumber EST collection version 3.0transcribed_cluster
CU166335cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa7G343870.1Csa7G343870.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU095147CU095147transcribed_cluster
CU148209CU148209transcribed_cluster
CU166335CU166335transcribed_cluster
CU140420CU140420transcribed_cluster
CU124644CU124644transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 268..558
score: 7.4E
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 29..191
score: 1.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..186
score: 1.9
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..186
score: 5.6
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 6..191
score: 5.0
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 30..190
score: 6.02
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 263..570
score: 2.51E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 264..572
score: 1.5E
NoneNo IPR availablePANTHERPTHR31707FAMILY NOT NAMEDcoord: 223..575
score: 1.8E-254coord: 3..189
score: 1.8E
NoneNo IPR availablePANTHERPTHR31707:SF55PECTINESTERASE 2-RELATEDcoord: 223..575
score: 1.8E-254coord: 3..189
score: 1.8E