Csa2G423680 (gene) Cucumber (Chinese Long) v2

NameCsa2G423680
Typegene
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionCytochrome P450, putative; contains IPR001128 (Cytochrome P450)
LocationChr2 : 22039401 .. 22039817 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTGAAAATTCGAGAAGATGAAGTCAAGTGTTTGCTAAGTAAGTTACATGGTAATCATTGTAAGTTGGAAGATGATGGGTTTAGAGTAGTGGAAATGGGACCATTGTTGAAAGATCTTACGTTGAATATTGTTATGAGAATGGTGAGTGGAAAAGTGTTTTATGAAGAAGATTCTAACAAGTTCAGAGAAATTGTGACAAAAATTATGGAACATGGTGGAGCAAGTAGTCCCGAAGATTTCATACCTTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAAGGTGAAGAAGCTTGGAAAGACATCAGATGAATTTCTTCAACTGTTAATTGATGGGATTAGGAATCAAAATGATGGAGGAAACACTATGATTCACCATTTACTTACCTTGCAGGATGTTGAAACCTGA

mRNA sequence

ATGTTCTTGAAAATTCGAGAAGATGAAGTCAAGTGTTTGCTAAGTAAGTTACATGGTAATCATTGTAAGTTGGAAGATGATGGGTTTAGAGTAGTGGAAATGGGACCATTGTTGAAAGATCTTACGTTGAATATTGTTATGAGAATGGTGAGTGGAAAAGTGTTTTATGAAGAAGATTCTAACAAGTTCAGAGAAATTGTGACAAAAATTATGGAACATGGTGGAGCAAGTAGTCCCGAAGATTTCATACCTTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAAGGTGAAGAAGCTTGGAAAGACATCAGATGAATTTCTTCAACTGTTAATTGATGGGATTAGGAATCAAAATGATGGAGGAAACACTATGATTCACCATTTACTTACCTTGCAGGATGTTGAAACCTGA

Coding sequence (CDS)

ATGTTCTTGAAAATTCGAGAAGATGAAGTCAAGTGTTTGCTAAGTAAGTTACATGGTAATCATTGTAAGTTGGAAGATGATGGGTTTAGAGTAGTGGAAATGGGACCATTGTTGAAAGATCTTACGTTGAATATTGTTATGAGAATGGTGAGTGGAAAAGTGTTTTATGAAGAAGATTCTAACAAGTTCAGAGAAATTGTGACAAAAATTATGGAACATGGTGGAGCAAGTAGTCCCGAAGATTTCATACCTTTATGGAATTGGATTGATCCAACTGGTTTTATGAAGAAGGTGAAGAAGCTTGGAAAGACATCAGATGAATTTCTTCAACTGTTAATTGATGGGATTAGGAATCAAAATGATGGAGGAAACACTATGATTCACCATTTACTTACCTTGCAGGATGTTGAAACCTGA

Protein sequence

MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQNDGGNTMIHHLLTLQDVET*
BLAST of Csa2G423680 vs. Swiss-Prot
Match: C81F2_ARATH (Cytochrome P450 81F2 OS=Arabidopsis thaliana GN=CYP81F2 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-21
Identity = 63/139 (45.32%), Postives = 89/139 (64.03%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R+DE++ LL+KL       E DG RVVE+ PLL DLT N ++RMV+G+ +Y     
Sbjct: 143 FLSVRKDEIRRLLTKL-----SREYDG-RVVELEPLLADLTFNNIVRMVTGRRYYGDQVH 202

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            +E++N F+++VT I ++ GAS P D++P+   +   G+ KKVK LG+  D FLQ L+D 
Sbjct: 203 NKEEANLFKKLVTDINDNSGASHPGDYLPILK-VFGHGYEKKVKALGEAMDAFLQRLLDE 262

Query: 122 IRNQNDGGNTMIHHLLTLQ 135
            R  N   NTM+ HLL+LQ
Sbjct: 263 CR-INGESNTMVSHLLSLQ 273

BLAST of Csa2G423680 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 4.4e-20
Identity = 57/141 (40.43%), Postives = 79/141 (56.03%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYE---- 61
           FL+IR DE+  L+ KL     +   +GF  VE+ P+  ++T N +MRMVSGK +Y     
Sbjct: 144 FLEIRRDEIMRLIQKL----AQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCD 203

Query: 62  ----EDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLI 121
               E++  FR I+ +++  GGA++  DF+    W D  G  K++KK+ K +D FLQ LI
Sbjct: 204 VSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLI 263

Query: 122 DGIRNQNDGGNTMIHHLLTLQ 135
           D  R      NTMI HLLT Q
Sbjct: 264 DEHRFGKRNSNTMIDHLLTQQ 280

BLAST of Csa2G423680 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.1e-18
Identity = 54/142 (38.03%), Postives = 82/142 (57.75%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           F+ IR+DE++ L+  L  N       GF  VEM PL   LT+N ++RMV+GK FY     
Sbjct: 154 FVSIRQDEIRRLIICLAKN----SQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTE 213

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            + ++   R+++ +++  GGA +  D+ P+  ++  T + K VKKL    DEFLQ L++ 
Sbjct: 214 NDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYV--TNYEKHVKKLAGRVDEFLQSLVNE 273

Query: 122 IRNQNDGGNTMIHHLLTLQDVE 138
            R +   GNTMI HLL+LQ+ +
Sbjct: 274 KRVEKVKGNTMIDHLLSLQETQ 289

BLAST of Csa2G423680 vs. Swiss-Prot
Match: C81F3_ARATH (Cytochrome P450 81F3 OS=Arabidopsis thaliana GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.1e-18
Identity = 56/142 (39.44%), Postives = 82/142 (57.75%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL IR+DE+  +L++L         D  + +E+ PLL DLT N ++RMV+GK +Y     
Sbjct: 144 FLHIRKDEIHRMLTRL-------SRDVNKEIELEPLLSDLTFNNIVRMVTGKRYYGDEVH 203

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            EE++N F+++V  I +  GA  P D++P       + F KKVK L +  DE LQ L++ 
Sbjct: 204 NEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALAEAMDEILQRLLEE 263

Query: 122 IRNQNDGGNTMIHHLLTLQDVE 138
            +   D GNTM++HLL+LQ  E
Sbjct: 264 CKRDKD-GNTMVNHLLSLQQNE 276

BLAST of Csa2G423680 vs. Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.1e-18
Identity = 56/144 (38.89%), Postives = 85/144 (59.03%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R DEV+ L+S+L     +L      VVE+ P+L DLT N +MRM++GK +Y     
Sbjct: 152 FLYVRTDEVRRLISRLS----RLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETT 211

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            EE++ + R++V  +  +  + +  D++P+      + +  +VKKLG+ +D+FLQ LID 
Sbjct: 212 DEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKLGEETDKFLQGLIDD 271

Query: 122 IRNQNDGGNTMIHHLLTLQ--DVE 138
            R Q + G TMI HLL LQ  D+E
Sbjct: 272 KRGQQETGTTMIDHLLVLQKSDIE 289

BLAST of Csa2G423680 vs. TrEMBL
Match: A0A0A0LN94_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423680 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 3.7e-74
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS
Sbjct: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN
Sbjct: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120

Query: 121 DGGNTMIHHLLTLQDVET 139
           DGGNTMIHHLLTLQDVET
Sbjct: 121 DGGNTMIHHLLTLQDVET 138

BLAST of Csa2G423680 vs. TrEMBL
Match: A0A0A0LQI5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.8e-33
Identity = 79/148 (53.38%), Postives = 104/148 (70.27%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           F +IRE+EVK LL KL GN+ K ED+ FRVVE+  +L DLT NIVMRMV GK F +E++ 
Sbjct: 144 FSRIREEEVKRLLCKLCGNY-KFEDE-FRVVELESMLLDLTSNIVMRMVGGKKFCKENNK 203

Query: 62  ------------KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFL 121
                       + +E+VT+IM H G+++P DFIPLWNWIDP+G+ K++ K+G+  DE L
Sbjct: 204 NINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYNKRIMKIGRRMDEVL 263

Query: 122 QLLIDGIRNQNDGGNTMIHHLLTLQDVE 138
           Q L+D IRN+ D GNTMI HLL LQ  +
Sbjct: 264 QRLVDEIRNEEDEGNTMIQHLLRLQKTD 289

BLAST of Csa2G423680 vs. TrEMBL
Match: A0A0A0LMZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423650 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.9e-32
Identity = 80/143 (55.94%), Postives = 99/143 (69.23%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGK------V 60
           +F+  REDEVK LL KL   +   E   F+VVEM P+  DL  NI+MRM+SGK      +
Sbjct: 143 LFVGGREDEVKRLLCKLWDGYSFEE---FKVVEMFPMFMDLMFNILMRMMSGKKNCGDDL 202

Query: 61  FYEEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLID 120
             EE S KF+E+V +++   GA +P DFIPLWNWIDPT + K+VKKL KTSDE +Q LID
Sbjct: 203 KDEERSKKFKEMVKQVLAIAGAGNPGDFIPLWNWIDPTCYNKRVKKLSKTSDELIQQLID 262

Query: 121 GIRNQNDGGNTMIHHLLTLQDVE 138
            IRNQNDG NTMI HLL LQ+ +
Sbjct: 263 EIRNQNDGENTMIQHLLGLQNTQ 282

BLAST of Csa2G423680 vs. TrEMBL
Match: A0A0A0LNA4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 7.7e-32
Identity = 75/140 (53.57%), Postives = 99/140 (70.71%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED- 60
           +F ++RE+EVK L+ +L G+      +GFR VEM  +L DL  N++M M+ GK   EED 
Sbjct: 142 LFSRMREEEVKALMVRLCGSSSL---EGFRAVEMESMLLDLMYNVIMGMMGGKKGCEEDE 201

Query: 61  --SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIR 120
             S +FRE+VTKIM  GGAS+P DFI +WNWIDP+G  KK+ KLG+T D  LQ L+DG+R
Sbjct: 202 GKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGLKKKILKLGQTMDVLLQELVDGMR 261

Query: 121 NQNDGGNTMIHHLLTLQDVE 138
           N++  GNTMIH LL LQ +E
Sbjct: 262 NESGEGNTMIHRLLQLQKIE 278

BLAST of Csa2G423680 vs. TrEMBL
Match: A0A0A0LTD0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.9e-31
Identity = 74/142 (52.11%), Postives = 97/142 (68.31%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED-- 61
           F  IR+DEVK LL KL   +   E   F  VE+ P+L D T NIVMRMV GK ++ +D  
Sbjct: 143 FSCIRQDEVKRLLHKLCNRNSVEELLLFSAVELEPILLDFTSNIVMRMVGGKRYFGDDVL 202

Query: 62  ----SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
               + KFR++V ++M + GA++P DFIPLWNWIDPTG  KK+ K+G+ +DE  Q LID 
Sbjct: 203 DEGQAEKFRDVVKRVMLYAGATNPGDFIPLWNWIDPTGLEKKIMKVGEEADEIFQGLIDE 262

Query: 122 IRNQNDGGNTMIHHLLTLQDVE 138
           IRN+ + GNTMIHHLL LQ+ +
Sbjct: 263 IRNEEEDGNTMIHHLLHLQNTQ 284

BLAST of Csa2G423680 vs. TAIR10
Match: AT5G57220.1 (AT5G57220.1 cytochrome P450, family 81, subfamily F, polypeptide 2)

HSP 1 Score: 103.6 bits (257), Expect = 1.0e-22
Identity = 63/139 (45.32%), Postives = 89/139 (64.03%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R+DE++ LL+KL       E DG RVVE+ PLL DLT N ++RMV+G+ +Y     
Sbjct: 143 FLSVRKDEIRRLLTKL-----SREYDG-RVVELEPLLADLTFNNIVRMVTGRRYYGDQVH 202

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            +E++N F+++VT I ++ GAS P D++P+   +   G+ KKVK LG+  D FLQ L+D 
Sbjct: 203 NKEEANLFKKLVTDINDNSGASHPGDYLPILK-VFGHGYEKKVKALGEAMDAFLQRLLDE 262

Query: 122 IRNQNDGGNTMIHHLLTLQ 135
            R  N   NTM+ HLL+LQ
Sbjct: 263 CR-INGESNTMVSHLLSLQ 273

BLAST of Csa2G423680 vs. TAIR10
Match: AT4G37310.1 (AT4G37310.1 cytochrome P450, family 81, subfamily H, polypeptide 1)

HSP 1 Score: 101.7 bits (252), Expect = 3.8e-22
Identity = 62/144 (43.06%), Postives = 81/144 (56.25%), Query Frame = 1

Query: 4   KIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED---- 63
           +IR DEVK LL KLH     L  +    VE+  LL  LTLN++MRM++GK F+EED    
Sbjct: 147 EIRHDEVKMLLQKLHD----LSVERPAKVELRQLLTGLTLNVIMRMMTGKRFFEEDDGGK 206

Query: 64  ---SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGI 123
              S +FRE+V +I+E   A +P DF+P   W D  G +K+ K++G+  D  LQ  +D  
Sbjct: 207 AGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKRIGERMDSLLQGFLDEH 266

Query: 124 RNQNDG---GNTMIHHLLTLQDVE 138
           R   D     NTMI HLL  Q+ E
Sbjct: 267 RANKDRLEFKNTMIAHLLDSQEKE 286

BLAST of Csa2G423680 vs. TAIR10
Match: AT2G23190.1 (AT2G23190.1 cytochrome P450, family 81, subfamily D, polypeptide 7)

HSP 1 Score: 99.0 bits (245), Expect = 2.5e-21
Identity = 57/142 (40.14%), Postives = 81/142 (57.04%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R+DE++ LL +L  N       GF  VEM  L  DLT+N ++RMV+GK FY     
Sbjct: 190 FLSVRKDEIRHLLLRLSKN----SQHGFAKVEMRQLFYDLTINNILRMVAGKRFYGEGTE 249

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            +E + +  +++ +I+   G  +  D+IP+  WI  T F K VK+L    DEFLQ L+D 
Sbjct: 250 QDEVARRVTQLIDEIVYRAGVGNAADYIPILRWI--TDFEKGVKELASRVDEFLQSLVDE 309

Query: 122 IRNQNDGGNTMIHHLLTLQDVE 138
            R     GNTM+ HLL+LQ+ +
Sbjct: 310 RRVHKQKGNTMMDHLLSLQETQ 325

BLAST of Csa2G423680 vs. TAIR10
Match: AT2G23220.1 (AT2G23220.1 cytochrome P450, family 81, subfamily D, polypeptide 6)

HSP 1 Score: 95.1 bits (235), Expect = 3.6e-20
Identity = 57/140 (40.71%), Postives = 82/140 (58.57%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL +R+DE++ LL +L  N       GF  VEM  LL +LT+N V RMV+GK FY     
Sbjct: 161 FLSVRKDEIRQLLLRLSKN----SRHGFAKVEMRHLLFELTINNVFRMVAGKRFYGEGTE 220

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            +E + + R ++ +I+   GA +  D++P+  W   T F K+VK L    D+FLQ L+D 
Sbjct: 221 QDEVAQQVRHLMDEIVTSAGAGNAADYLPIMRWF--TNFEKRVKNLAIRIDKFLQSLVDE 280

Query: 122 IRNQNDGGNTMIHHLLTLQD 136
            R   + G TMI HLL+LQ+
Sbjct: 281 KRADKEKGTTMIDHLLSLQE 294

BLAST of Csa2G423680 vs. TAIR10
Match: AT4G37400.1 (AT4G37400.1 cytochrome P450, family 81, subfamily F, polypeptide 3)

HSP 1 Score: 92.8 bits (229), Expect = 1.8e-19
Identity = 56/142 (39.44%), Postives = 82/142 (57.75%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFY----- 61
           FL IR+DE+  +L++L         D  + +E+ PLL DLT N ++RMV+GK +Y     
Sbjct: 144 FLHIRKDEIHRMLTRL-------SRDVNKEIELEPLLSDLTFNNIVRMVTGKRYYGDEVH 203

Query: 62  -EEDSNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDG 121
            EE++N F+++V  I +  GA  P D++P       + F KKVK L +  DE LQ L++ 
Sbjct: 204 NEEEANVFKKLVADINDCSGARHPGDYLPFMKMFGGS-FEKKVKALAEAMDEILQRLLEE 263

Query: 122 IRNQNDGGNTMIHHLLTLQDVE 138
            +   D GNTM++HLL+LQ  E
Sbjct: 264 CKRDKD-GNTMVNHLLSLQQNE 276

BLAST of Csa2G423680 vs. NCBI nr
Match: gi|700208169|gb|KGN63288.1| (hypothetical protein Csa_2G423680 [Cucumis sativus])

HSP 1 Score: 285.4 bits (729), Expect = 5.3e-74
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS
Sbjct: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN
Sbjct: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120

Query: 121 DGGNTMIHHLLTLQDVET 139
           DGGNTMIHHLLTLQDVET
Sbjct: 121 DGGNTMIHHLLTLQDVET 138

BLAST of Csa2G423680 vs. NCBI nr
Match: gi|659111621|ref|XP_008455824.1| (PREDICTED: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 252.3 bits (643), Expect = 4.9e-64
Identity = 124/137 (90.51%), Postives = 129/137 (94.16%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDS 60
           MFL IREDEVK LL KL GNHCKLEDD FRVVE+GPLLKDLTLNIVMRMVSGK+FYEEDS
Sbjct: 142 MFLGIREDEVKRLLRKLCGNHCKLEDDEFRVVEIGPLLKDLTLNIVMRMVSGKMFYEEDS 201

Query: 61  NKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIRNQN 120
           NKFREIVT+IMEH GAS+P DFIPLWNWIDPTGFMKKVKKLGKTSDEFLQ LIDGIRNQN
Sbjct: 202 NKFREIVTQIMEHAGASNPGDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQQLIDGIRNQN 261

Query: 121 DGGNTMIHHLLTLQDVE 138
           DGGNTMIHHLLTLQ+VE
Sbjct: 262 DGGNTMIHHLLTLQNVE 278

BLAST of Csa2G423680 vs. NCBI nr
Match: gi|659111617|ref|XP_008455822.1| (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81D1-like [Cucumis melo])

HSP 1 Score: 156.4 bits (394), Expect = 3.7e-35
Identity = 83/146 (56.85%), Postives = 106/146 (72.60%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           F + RE+EVK LL KL GN+ KLED+ FRVVE+ P+L DLT NIVMRMV GK F EE +N
Sbjct: 144 FSRTREEEVKRLLRKLWGNY-KLEDE-FRVVELEPMLLDLTFNIVMRMVGGKKFCEEKNN 203

Query: 62  ----------KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQL 121
                     +F+E+VT+IM HGG+++P DFIPL NWIDP+G+ K + K+G+ +DE LQ 
Sbjct: 204 NVLEDEGYCKRFKELVTQIMAHGGSTNPGDFIPLLNWIDPSGYEKDIMKIGRKTDEVLQG 263

Query: 122 LIDGIRNQNDGGNTMIHHLLTLQDVE 138
           L+D IRN+ D GNTMI HLL LQ  +
Sbjct: 264 LVDEIRNEEDEGNTMIQHLLRLQKTD 287

BLAST of Csa2G423680 vs. NCBI nr
Match: gi|778674449|ref|XP_004151924.2| (PREDICTED: cytochrome P450 81D1-like [Cucumis sativus])

HSP 1 Score: 150.2 bits (378), Expect = 2.6e-33
Identity = 79/148 (53.38%), Postives = 104/148 (70.27%), Query Frame = 1

Query: 2   FLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEEDSN 61
           F +IRE+EVK LL KL GN+ K ED+ FRVVE+  +L DLT NIVMRMV GK F +E++ 
Sbjct: 144 FSRIREEEVKRLLCKLCGNY-KFEDE-FRVVELESMLLDLTSNIVMRMVGGKKFCKENNK 203

Query: 62  ------------KFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFL 121
                       + +E+VT+IM H G+++P DFIPLWNWIDP+G+ K++ K+G+  DE L
Sbjct: 204 NINVLEDEGYCKRLKELVTQIMAHAGSTNPGDFIPLWNWIDPSGYNKRIMKIGRRMDEVL 263

Query: 122 QLLIDGIRNQNDGGNTMIHHLLTLQDVE 138
           Q L+D IRN+ D GNTMI HLL LQ  +
Sbjct: 264 QRLVDEIRNEEDEGNTMIQHLLRLQKTD 289

BLAST of Csa2G423680 vs. NCBI nr
Match: gi|659111602|ref|XP_008455814.1| (PREDICTED: LOW QUALITY PROTEIN: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 149.8 bits (377), Expect = 3.5e-33
Identity = 77/140 (55.00%), Postives = 101/140 (72.14%), Query Frame = 1

Query: 1   MFLKIREDEVKCLLSKLHGNHCKLEDDGFRVVEMGPLLKDLTLNIVMRMVSGKVFYEED- 60
           +F ++RE+EVK L+ +L G+      +GFR VEM  +L DL  N++MRM+ GK   EED 
Sbjct: 143 LFSRMREEEVKALMVRLCGSSSL---EGFRAVEMESMLLDLMYNVIMRMMGGKKDCEEDE 202

Query: 61  --SNKFREIVTKIMEHGGASSPEDFIPLWNWIDPTGFMKKVKKLGKTSDEFLQLLIDGIR 120
             S +FRE+VTKIM  GGAS+P DFI +WNWIDP+G+ KK+ KLG+T D  LQ L+DGIR
Sbjct: 203 GKSKEFREMVTKIMAVGGASNPGDFIAIWNWIDPSGWKKKIMKLGQTMDVLLQDLVDGIR 262

Query: 121 NQNDGGNTMIHHLLTLQDVE 138
           N+N  GNTM+H LL LQ +E
Sbjct: 263 NENGEGNTMLHRLLHLQKIE 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C81F2_ARATH1.8e-2145.32Cytochrome P450 81F2 OS=Arabidopsis thaliana GN=CYP81F2 PE=2 SV=1[more]
C81E8_MEDTR4.4e-2040.43Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1[more]
C8D11_ARATH3.1e-1838.03Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1[more]
C81F3_ARATH3.1e-1839.44Cytochrome P450 81F3 OS=Arabidopsis thaliana GN=CYP81F3 PE=2 SV=1[more]
C81D1_ARATH4.1e-1838.89Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LN94_CUCSA3.7e-74100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423680 PE=4 SV=1[more]
A0A0A0LQI5_CUCSA1.8e-3353.38Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423640 PE=3 SV=1[more]
A0A0A0LMZ5_CUCSA5.9e-3255.94Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423650 PE=4 SV=1[more]
A0A0A0LNA4_CUCSA7.7e-3253.57Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425750 PE=3 SV=1[more]
A0A0A0LTD0_CUCSA2.9e-3152.11Uncharacterized protein OS=Cucumis sativus GN=Csa_2G425740 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57220.11.0e-2245.32 cytochrome P450, family 81, subfamily F, polypeptide 2[more]
AT4G37310.13.8e-2243.06 cytochrome P450, family 81, subfamily H, polypeptide 1[more]
AT2G23190.12.5e-2140.14 cytochrome P450, family 81, subfamily D, polypeptide 7[more]
AT2G23220.13.6e-2040.71 cytochrome P450, family 81, subfamily D, polypeptide 6[more]
AT4G37400.11.8e-1939.44 cytochrome P450, family 81, subfamily F, polypeptide 3[more]
Match NameE-valueIdentityDescription
gi|700208169|gb|KGN63288.1|5.3e-74100.00hypothetical protein Csa_2G423680 [Cucumis sativus][more]
gi|659111621|ref|XP_008455824.1|4.9e-6490.51PREDICTED: cytochrome P450 81D1-like [Cucumis melo][more]
gi|659111617|ref|XP_008455822.1|3.7e-3556.85PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 81D1-like [Cucumis melo][more]
gi|778674449|ref|XP_004151924.2|2.6e-3353.38PREDICTED: cytochrome P450 81D1-like [Cucumis sativus][more]
gi|659111602|ref|XP_008455814.1|3.5e-3355.00PREDICTED: LOW QUALITY PROTEIN: isoflavone 2'-hydroxylase-like [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006810 transport
cellular_component GO:0005575 cellular_component
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0005215 transporter activity
molecular_function GO:0004497 monooxygenase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
CU140533cucumber EST collection version 3.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csa2G423680.1Csa2G423680.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
CU140533CU140533transcribed_cluster


Analysis Name: InterPro Annotations of cucumber (Chinese Long)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 3..135
score: 2.5
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..135
score: 1.16
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..137
score: 1.6
NoneNo IPR availablePANTHERPTHR24298:SF61CYTOCHROME P450 81D1-RELATEDcoord: 1..137
score: 1.6