CU140533 (transcribed_cluster) Cucumber (Chinese Long) v2

NameCU140533
Typetranscribed_cluster
OrganismCucumis. sativus (Cucumber (Chinese Long) v2)
DescriptionUnknown protein
LocationChr2 : 22037027 .. 22045211 (+)
Sequence length185
The following sequences are available for this feature:

transcribed_cluster sequence

TCTTGTTTTCTTTTCTGGAAACTGAGATAGAATCATATAAATTACCAATCTAAAGCAGTGATGGTAACTCCAGCCCAGAGAGGCAAAGCTCCATTACTCAGAATCTTAATAGCAATAGCACTCCCAATGACTTCCTGTATATCAGCCCCAATGAGGGCAAACTCCGCCATGACCCACGGCACAAT
Library categoryESTs
gynoecious flower library1
BLAST of CU140533 vs. Swiss-Prot
Match: NRAM3_ARATH (Metal transporter Nramp3 OS=Arabidopsis thaliana GN=NRAMP3 PE=2 SV=2)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-14
Identity = 40/46 (86.96%), Postives = 40/46 (86.96%), Query Frame = -1

Query: 48  IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALD 107
           +V WVMAE ALIG+DIQEVIGSAIAIKILSNG LPLWAGV ITALD
Sbjct: 133 MVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGVVITALD 178

Query: 108 167
           
Sbjct: 193 178

Query: 168 186
           
Sbjct: 253 178

BLAST of CU140533 vs. Swiss-Prot
Match: NRAM4_ARATH (Metal transporter Nramp4 OS=Arabidopsis thaliana GN=NRAMP4 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.2e-14
Identity = 42/60 (70.00%), Postives = 45/60 (75.00%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V W+MAE ALIGADIQEVIGSAIAIKILSNG +PLWAGV ITALD     F  ++  RK
Sbjct: 129 MVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALDCFIFLFLENYGIRK 188

Query: 66  125
           
Sbjct: 189 188

Query: 126 185
           
Sbjct: 249 188

BLAST of CU140533 vs. Swiss-Prot
Match: NRAM6_ORYSJ (Metal transporter Nramp6 OS=Oryza sativa subsp. japonica GN=NRAMP6 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.9e-13
Identity = 36/43 (83.72%), Postives = 37/43 (86.05%), Query Frame = -1

Query: 48  WVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALD 107
           W+MAE A++GADIQEVIGSAIAIKILS G LPLWAGV ITALD
Sbjct: 167 WLMAEVAMVGADIQEVIGSAIAIKILSRGFLPLWAGVVITALD 209

Query: 108 167
           
Sbjct: 227 209

Query: 168 177
           
Sbjct: 287 209

BLAST of CU140533 vs. Swiss-Prot
Match: NRAM2_ARATH (Metal transporter Nramp2 OS=Arabidopsis thaliana GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.9e-12
Identity = 40/59 (67.80%), Postives = 41/59 (69.49%), Query Frame = -1

Query: 6   VPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           V W MAE ALIGADIQEVIGSAIAI+ILS G LPLWAGV ITA D     F  ++  RK
Sbjct: 146 VLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASDCFLFLFLENYGVRK 204

Query: 66  125
           
Sbjct: 206 204

Query: 126 183
           
Sbjct: 266 204

BLAST of CU140533 vs. Swiss-Prot
Match: NRAM2_ORYSJ (Metal transporter Nramp2 OS=Oryza sativa subsp. japonica GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 7.3e-12
Identity = 36/57 (63.16%), Postives = 38/57 (66.67%), Query Frame = -1

Query: 6   WVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           W M E AL+GADIQEVIGSAIAIKILS G +PLW GV ITA D     F  ++  RK
Sbjct: 150 WAMTELALVGADIQEVIGSAIAIKILSAGTVPLWGGVVITAFDCFIFLFLENYGVRK 206

Query: 66  125
           
Sbjct: 210 206

Query: 126 177
           
Sbjct: 270 206

BLAST of CU140533 vs. TrEMBL
Match: A0A0A0LN03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423700 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 2.2e-15
Identity = 48/60 (80.00%), Postives = 50/60 (83.33%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           IV WVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALD     F  ++  RK
Sbjct: 128 IVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDCFIFLFLENYGVRK 187

Query: 66  125
           
Sbjct: 188 187

Query: 126 185
           
Sbjct: 248 187

BLAST of CU140533 vs. TrEMBL
Match: M5VXT2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004490mg PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.1e-13
Identity = 44/60 (73.33%), Postives = 48/60 (80.00%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIGADIQEVIGSAIAIKILSNGALPLW+GV ITALD     F  ++  RK
Sbjct: 130 MVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWSGVVITALDCFIFLFLENYGVRK 189

Query: 66  125
           
Sbjct: 190 189

Query: 126 185
           
Sbjct: 250 189

BLAST of CU140533 vs. TrEMBL
Match: Q3ZN86_NOCCA (Nramp metal transporter-like protein OS=Noccaea caerulescens GN=Nramp3 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.1e-13
Identity = 43/60 (71.67%), Postives = 49/60 (81.67%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIG+DIQEVIGSAIAIKIL+NG LPLWAGV ITALD  F  F+ ++  RK
Sbjct: 137 MVLWVMAELALIGSDIQEVIGSAIAIKILTNGILPLWAGVIITALDCFFFLFFENYGIRK 196

Query: 66  125
           
Sbjct: 197 196

Query: 126 185
           
Sbjct: 257 196

BLAST of CU140533 vs. TrEMBL
Match: A6YPU3_NOCCA (Metal transporter NRAMP3 OS=Noccaea caerulescens GN=NRAMP3 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.1e-13
Identity = 43/60 (71.67%), Postives = 49/60 (81.67%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIG+DIQEVIGSAIAIKIL+NG LPLWAGV ITALD  F  F+ ++  RK
Sbjct: 137 MVLWVMAELALIGSDIQEVIGSAIAIKILTNGILPLWAGVIITALDCFFFLFFENYGIRK 196

Query: 66  125
           
Sbjct: 197 196

Query: 126 185
           
Sbjct: 257 196

BLAST of CU140533 vs. TrEMBL
Match: A0A165FX05_9ROSA (Metal transporter Nramp3 OS=Pyrus betulifolia GN=Nramp3 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.7e-13
Identity = 44/60 (73.33%), Postives = 48/60 (80.00%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIGADIQEVIGSAIAIKILSNGALPLW+GV ITALD     F  ++  RK
Sbjct: 130 MVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWSGVLITALDCFIFLFLENYGVRK 189

Query: 66  125
           
Sbjct: 190 189

Query: 126 185
           
Sbjct: 250 189

BLAST of CU140533 vs. NCBI nr
Match: gi|449468323|ref|XP_004151871.1| (PREDICTED: metal transporter Nramp3-like [Cucumis sativus])

HSP 1 Score: 89.0 bits (219), Expect = 3.2e-15
Identity = 48/60 (80.00%), Postives = 50/60 (83.33%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           IV WVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALD     F  ++  RK
Sbjct: 128 IVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDCFIFLFLENYGVRK 187

Query: 66  125
           
Sbjct: 188 187

Query: 126 185
           
Sbjct: 248 187

BLAST of CU140533 vs. NCBI nr
Match: gi|659111615|ref|XP_008455821.1| (PREDICTED: metal transporter Nramp3-like [Cucumis melo])

HSP 1 Score: 86.7 bits (213), Expect = 1.6e-14
Identity = 47/60 (78.33%), Postives = 49/60 (81.67%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           IV WVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGV ITALD     F  ++  RK
Sbjct: 128 IVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRK 187

Query: 66  125
           
Sbjct: 188 187

Query: 126 185
           
Sbjct: 248 187

BLAST of CU140533 vs. NCBI nr
Match: gi|57231016|gb|AAW47269.1| (Nramp metal transporter-like protein [Noccaea caerulescens])

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-13
Identity = 43/60 (71.67%), Postives = 49/60 (81.67%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIG+DIQEVIGSAIAIKIL+NG LPLWAGV ITALD  F  F+ ++  RK
Sbjct: 137 MVLWVMAELALIGSDIQEVIGSAIAIKILTNGILPLWAGVIITALDCFFFLFFENYGIRK 196

Query: 66  125
           
Sbjct: 197 196

Query: 126 185
           
Sbjct: 257 196

BLAST of CU140533 vs. NCBI nr
Match: gi|645220057|ref|XP_008238525.1| (PREDICTED: metal transporter Nramp3 [Prunus mume])

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-13
Identity = 44/60 (73.33%), Postives = 48/60 (80.00%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIGADIQEVIGSAIAIKILSNGALPLW+GV ITALD     F  ++  RK
Sbjct: 130 MVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWSGVVITALDCFIFLFLENYGVRK 189

Query: 66  125
           
Sbjct: 190 189

Query: 126 185
           
Sbjct: 250 189

BLAST of CU140533 vs. NCBI nr
Match: gi|595823396|ref|XP_007205063.1| (hypothetical protein PRUPE_ppa004490mg [Prunus persica])

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-13
Identity = 44/60 (73.33%), Postives = 48/60 (80.00%), Query Frame = -1

Query: 6   IVPWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDW*FI*FYLSFQKRK 65
           +V WVMAE ALIGADIQEVIGSAIAIKILSNGALPLW+GV ITALD     F  ++  RK
Sbjct: 130 MVLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWSGVVITALDCFIFLFLENYGVRK 189

Query: 66  125
           
Sbjct: 190 189

Query: 126 185
           
Sbjct: 250 189

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NRAM3_ARATH1.2e-1486.96Metal transporter Nramp3 OS=Arabidopsis thaliana GN=NRAMP3 PE=2 SV=2[more]
NRAM4_ARATH1.2e-1470.00Metal transporter Nramp4 OS=Arabidopsis thaliana GN=NRAMP4 PE=2 SV=1[more]
NRAM6_ORYSJ3.9e-1383.72Metal transporter Nramp6 OS=Oryza sativa subsp. japonica GN=NRAMP6 PE=2 SV=1[more]
NRAM2_ARATH1.9e-1267.80Metal transporter Nramp2 OS=Arabidopsis thaliana GN=NRAMP2 PE=2 SV=1[more]
NRAM2_ORYSJ7.3e-1263.16Metal transporter Nramp2 OS=Oryza sativa subsp. japonica GN=NRAMP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LN03_CUCSA2.2e-1580.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G423700 PE=3 SV=1[more]
M5VXT2_PRUPE2.1e-1373.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004490mg PE=3 SV=1[more]
Q3ZN86_NOCCA2.1e-1371.67Nramp metal transporter-like protein OS=Noccaea caerulescens GN=Nramp3 PE=2 SV=1[more]
A6YPU3_NOCCA2.1e-1371.67Metal transporter NRAMP3 OS=Noccaea caerulescens GN=NRAMP3 PE=2 SV=1[more]
A0A165FX05_9ROSA2.7e-1373.33Metal transporter Nramp3 OS=Pyrus betulifolia GN=Nramp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
gi|449468323|ref|XP_004151871.1|3.2e-1580.00PREDICTED: metal transporter Nramp3-like [Cucumis sativus][more]
gi|659111615|ref|XP_008455821.1|1.6e-1478.33PREDICTED: metal transporter Nramp3-like [Cucumis melo][more]
gi|57231016|gb|AAW47269.1|3.0e-1371.67Nramp metal transporter-like protein [Noccaea caerulescens][more]
gi|645220057|ref|XP_008238525.1|3.0e-1373.33PREDICTED: metal transporter Nramp3 [Prunus mume][more]
gi|595823396|ref|XP_007205063.1|3.0e-1373.33hypothetical protein PRUPE_ppa004490mg [Prunus persica][more]
The following terms have been associated with this transcribed_cluster:
Vocabulary: INTERPRO
TermDefinition
IPR001046NRAMP_fam
Vocabulary: Molecular Function
TermDefinition
GO:0005215transporter activity
Vocabulary: Biological Process
TermDefinition
GO:0006810transport
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
GO Assignments
This transcribed_cluster is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006810 transport
cellular_component GO:0016020 membrane
molecular_function GO:0005215 transporter activity

This transcribed_cluster is associated with the following gene feature(s):

Feature NameUnique NameType
Csa2G423700Csa2G423700gene
Csa2G423680Csa2G423680gene
Csa2G423690Csa2G423690gene


The following EST feature(s) are a part of this transcribed_cluster:

Feature NameUnique NameType
G0074472G0074472EST


Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 3..29
score: 4.7E-15coord: 33..47
score: 4.7
IPR001046NRAMP familyPANTHERPTHR11706MANGANESE TRANSPORTERcoord: 4..46
score: 5.3
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 3..46
score: 3.
NoneNo IPR availablePANTHERPTHR11706:SF8METAL TRANSPORTER NRAMP1 HOMOLOGcoord: 4..46
score: 5.3