CU176807 (transcribed_cluster) Cucumber (Chinese Long) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CU176807
Type	transcribed_cluster
Organism	Cucumis. sativus (Cucumber (Chinese Long) v2)
Description	Unknown protein
Location	Chr1 : 27792156 .. 27800505 (-)
Sequence length	250

Genome Viewer

Sequences

The following sequences are available for this feature:

transcribed_cluster sequence

CATATGAGATCAATTCCCACTCGTAGTCCTTTGGTCTTTGAAAAAACTCTCCGATAGCTCCCCAGTCATACTGGTAATCGAAAATTCCTTTACATTATGTACAGAATTCGTCAAGCTGATCGAGTCCTTAGTCGCTAAATCGTTATACACTTCAGCAATATCATTTGTGGGATAGAGTACTCTTGATCCAGGCAAAGGAAGAACAACATCATCAATAGTAAAGTTTCCACTGAGGACATCTCCAGCAGTA

Library


Library category	ESTs
hermaphrodite flower library	1

Homology

[Close]

BLAST of CU176807 vs. Swiss-Prot
Match: PUS7_BOVIN (Pseudouridylate synthase 7 homolog OS=Bos taurus GN=PUS7 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 7.4e-07
Identity = 20/51 (39.22%), Postives = 35/51 (68.63%), Query Frame = -2


Query: 73  NFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSIT 132
           N++I DVV+PLPG  V+YP + I+E Y ++ T D++ + N  H ++++S++
Sbjct: 519 NYSIHDVVMPLPGFDVIYPKHKISEAYREMLTADNLDIDNMRHKIRDYSLS 569

Query: 133  192
           
Sbjct: 579  569

Query: 193  226
           
Sbjct: 639  569

[Close]

BLAST of CU176807 vs. Swiss-Prot
Match: PUS7_HUMAN (Pseudouridylate synthase 7 homolog OS=Homo sapiens GN=PUS7 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 20/51 (39.22%), Postives = 34/51 (66.67%), Query Frame = -2


Query: 73  NFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSIT 132
           N++I DVV+PLPG  V+YP + I E Y ++ T D++ + N  H ++++S++
Sbjct: 521 NYSIHDVVMPLPGFDVIYPKHKIQEAYREMLTADNLDIDNMRHKIRDYSLS 571

Query: 133  192
           
Sbjct: 581  571

Query: 193  226
           
Sbjct: 641  571

[Close]

BLAST of CU176807 vs. TrEMBL
Match: M5WRD4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020985mg PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 8.2e-21
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+LSGN+TIDDVVLP+PGSRV++P NDIA V++DLA KD+ISLT SVHNVKEFSITS
Sbjct: 492 TAEDILSGNYTIDDVVLPMPGSRVIFPENDIAHVFHDLAKKDAISLTESVHNVKEFSITS 551

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 552 MTGSYRRVFQK 562

Query: 157  216
           
Sbjct: 612  562

Query: 217  250
           
Sbjct: 672  562

[Close]

BLAST of CU176807 vs. TrEMBL
Match: W9QVC1_9ROSA (Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 2.4e-20
Identity = 52/70 (74.29%), Postives = 58/70 (82.86%), Query Frame = -2


Query: 37  AGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITSM 96
           A D+L+GNF IDDVVLPLPGSRV+ PTNDIA+VY+DLA KD+ISLT S HNVKEFSITSM
Sbjct: 552 AEDILAGNFAIDDVVLPLPGSRVILPTNDIAQVYHDLAKKDAISLTESAHNVKEFSITSM 611

Query: 97  TGELSESFFK 156
           TG     F K
Sbjct: 612 TGSYRRVFQK 621

Query: 157  216
           
Sbjct: 672  621

Query: 217  247
           
Sbjct: 732  621

[Close]

BLAST of CU176807 vs. TrEMBL
Match: W9QVC1_9ROSA (Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 SV=1)

HSP 1 Score: 31.2 bits (69), Expect = 7.4e+02
Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = -1


Query: 2   GAIGEFFQRPKDYEWELISY 61
           G+    FQ+P DYEWEL++Y
Sbjct: 613 GSYRRVFQKPVDYEWELLTY 632

HSP 2 Score: 104.8 bits (260), Expect = 5.3e-20
Identity = 48/71 (67.61%), Postives = 58/71 (81.69%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+ +GN+TI+DVVLP+PGSR++YP NDIA VY+D+A KD ISLT SVHN+KEFSITS
Sbjct: 561 TAADISTGNYTIEDVVLPMPGSRIIYPVNDIANVYHDMAKKDGISLTESVHNIKEFSITS 620

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 621 MTGSYRRVFQK 631

Query: 157  216
           
Sbjct: 681  631

Query: 217  250
           
Sbjct: 741  631

[Close]

BLAST of CU176807 vs. TrEMBL
Match: A0A061F9D9_THECC (Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026386 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 5.3e-20
Identity = 48/71 (67.61%), Postives = 58/71 (81.69%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+ +GN+TI+DVVLP+PGSR++YP NDIA VY+D+A KD ISLT SVHN+KEFSITS
Sbjct: 530 TAADISTGNYTIEDVVLPMPGSRIIYPVNDIANVYHDMAKKDGISLTESVHNIKEFSITS 589

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 590 MTGSYRRVFQK 600

Query: 157  216
           
Sbjct: 650  600

Query: 217  250
           
Sbjct: 710  600

[Close]

BLAST of CU176807 vs. TrEMBL
Match: A0A061F1A0_THECC (Pseudouridine synthase family protein isoform 3 OS=Theobroma cacao GN=TCM_026386 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 5.3e-20
Identity = 48/71 (67.61%), Postives = 58/71 (81.69%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+ +GN+TI+DVVLP+PGSR++YP NDIA VY+D+A KD ISLT SVHN+KEFSITS
Sbjct: 470 TAADISTGNYTIEDVVLPMPGSRIIYPVNDIANVYHDMAKKDGISLTESVHNIKEFSITS 529

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 530 MTGSYRRVFQK 540

Query: 157  216
           
Sbjct: 590  540

Query: 217  250
           
Sbjct: 650  540

[Close]

BLAST of CU176807 vs. NCBI nr
Match: gi|778664378|ref|XP_011660284.1| (PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus])

HSP 1 Score: 126.3 bits (316), Expect = 2.4e-26
Identity = 64/69 (92.75%), Postives = 64/69 (92.75%), Query Frame = -2


Query: 43  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 102
           TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS
Sbjct: 530 TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 589

Query: 103 MTGELSESF 162
           MTG     F
Sbjct: 590 MTGSYRRVF 598

Query: 163  222
           
Sbjct: 650  598

Query: 223  250
           
Sbjct: 710  598

[Close]

BLAST of CU176807 vs. NCBI nr
Match: gi|659125504|ref|XP_008462716.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo])

HSP 1 Score: 119.0 bits (297), Expect = 3.9e-24
Identity = 61/69 (88.41%), Postives = 61/69 (88.41%), Query Frame = -2


Query: 43  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 102
           TA DVLSGNFTIDDVVLPLPGSRVLYP NDIAEVYNDLATKDSISLT SVHNVKEFSITS
Sbjct: 530 TAEDVLSGNFTIDDVVLPLPGSRVLYPANDIAEVYNDLATKDSISLTESVHNVKEFSITS 589

Query: 103 MTGELSESF 162
           MTG     F
Sbjct: 590 MTGSYRRVF 598

Query: 163  222
           
Sbjct: 650  598

Query: 223  250
           
Sbjct: 710  598

[Close]

BLAST of CU176807 vs. NCBI nr
Match: gi|659125508|ref|XP_008462718.1| (PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo])

HSP 1 Score: 119.0 bits (297), Expect = 3.9e-24
Identity = 61/69 (88.41%), Postives = 61/69 (88.41%), Query Frame = -2


Query: 43  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 102
           TA DVLSGNFTIDDVVLPLPGSRVLYP NDIAEVYNDLATKDSISLT SVHNVKEFSITS
Sbjct: 500 TAEDVLSGNFTIDDVVLPLPGSRVLYPANDIAEVYNDLATKDSISLTESVHNVKEFSITS 559

Query: 103 MTGELSESF 162
           MTG     F
Sbjct: 560 MTGSYRRVF 568

Query: 163  222
           
Sbjct: 620  568

Query: 223  250
           
Sbjct: 680  568

[Close]

BLAST of CU176807 vs. NCBI nr
Match: gi|595841315|ref|XP_007208186.1| (hypothetical protein PRUPE_ppa020985mg [Prunus persica])

HSP 1 Score: 105.9 bits (263), Expect = 3.4e-20
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+LSGN+TIDDVVLP+PGSRV++P NDIA V++DLA KD+ISLT SVHNVKEFSITS
Sbjct: 492 TAEDILSGNYTIDDVVLPMPGSRVIFPENDIAHVFHDLAKKDAISLTESVHNVKEFSITS 551

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 552 MTGSYRRVFQK 562

Query: 157  216
           
Sbjct: 612  562

Query: 217  250
           
Sbjct: 672  562

[Close]

BLAST of CU176807 vs. NCBI nr
Match: gi|645222603|ref|XP_008218240.1| (PREDICTED: multisubstrate pseudouridine synthase 7 isoform X2 [Prunus mume])

HSP 1 Score: 105.9 bits (263), Expect = 3.4e-20
Identity = 52/71 (73.24%), Postives = 60/71 (84.51%), Query Frame = -2


Query: 37  TAGDVLSGNFTIDDVVLPLPGSRVLYPTNDIAEVYNDLATKDSISLTNSVHNVKEFSITS 96
           TA D+LSGN+TIDDVVLP+PGSRV++P NDIA V++DLA KD+ISLT SVHNVKEFSITS
Sbjct: 522 TAEDILSGNYTIDDVVLPMPGSRVIFPENDIAHVFHDLAKKDAISLTESVHNVKEFSITS 581

Query: 97  MTGELSESFFK 156
           MTG     F K
Sbjct: 582 MTGSYRRVFQK 592

Query: 157  216
           
Sbjct: 642  592

Query: 217  250
           
Sbjct: 702  592

The following BLAST results are available for this feature:

BLAST of CU176807 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PUS7_BOVIN	7.4e-07	39.22	Pseudouridylate synthase 7 homolog OS=Bos taurus GN=PUS7 PE=2 SV=1	[more]
PUS7_HUMAN	1.3e-06	39.22	Pseudouridylate synthase 7 homolog OS=Homo sapiens GN=PUS7 PE=1 SV=2	[more]

BLAST of CU176807 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WRD4_PRUPE	8.2e-21	73.24	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa020985mg PE=3 SV=1	[more]
W9QVC1_9ROSA	2.4e-20	74.29	Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 S...	[more]
W9QVC1_9ROSA	7.4e+02	55.00	Multisubstrate pseudouridine synthase 7 OS=Morus notabilis GN=L484_006163 PE=4 S...	[more]
A0A061F9D9_THECC	5.3e-20	67.61	Pseudouridine synthase family protein isoform 2 OS=Theobroma cacao GN=TCM_026386...	[more]
A0A061F1A0_THECC	5.3e-20	67.61	Pseudouridine synthase family protein isoform 3 OS=Theobroma cacao GN=TCM_026386...	[more]

BLAST of CU176807 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778664378\|ref\|XP_011660284.1\|	2.4e-26	92.75	PREDICTED: pseudouridylate synthase 7 homolog [Cucumis sativus]	[more]
gi\|659125504\|ref\|XP_008462716.1\|	3.9e-24	88.41	PREDICTED: pseudouridylate synthase 7 homolog isoform X1 [Cucumis melo]	[more]
gi\|659125508\|ref\|XP_008462718.1\|	3.9e-24	88.41	PREDICTED: pseudouridylate synthase 7 homolog isoform X2 [Cucumis melo]	[more]
gi\|595841315\|ref\|XP_007208186.1\|	3.4e-20	73.24	hypothetical protein PRUPE_ppa020985mg [Prunus persica]	[more]
gi\|645222603\|ref\|XP_008218240.1\|	3.4e-20	73.24	PREDICTED: multisubstrate pseudouridine synthase 7 isoform X2 [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this transcribed_cluster:

Vocabulary: **INTERPRO**
Term	Definition
IPR001656	PsdUridine_synth_TruD
IPR020103	PsdUridine_synth_cat_dom_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0001522	pseudouridine synthesis
GO:0009451	RNA modification

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0009982	pseudouridine synthase activity

GO Annotation

GO Assignments

This transcribed_cluster is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding

Relationships

This transcribed_cluster is associated with the following gene feature(s):


Feature Name	Unique Name	Type
Csa1G690350	Csa1G690350	gene
Csa1G690330	Csa1G690330	gene
Csa1G690340	Csa1G690340	gene
Csa1G690320	Csa1G690320	gene

The following EST feature(s) are a part of this transcribed_cluster:


Feature Name	Unique Name	Type
H0184970	H0184970	EST

InterPro

Analysis Name: InterPro Annotations of cucumber unigene v3
Date Performed: 2016-11-16


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001656	Pseudouridine synthase, TruD	PANTHER	PTHR13326	TRNA PSEUDOURIDINE SYNTHASE D	coord: 2..84 score: 2.7
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 9..84 score: 3.
None	No IPR available	PANTHER	PTHR13326:SF1	PSEUDOURIDYLATE SYNTHASE 7 HOMOLOG	coord: 2..84 score: 2.7