MELO3C032832.2 (gene) Melon (DHL92) v3.6.1

Overview
NameMELO3C032832.2
Typegene
OrganismCucumis melo (Melon (DHL92) v3.6.1)
Descriptiongibberellin 2-beta-dioxygenase 8
Locationchr07 : 26056883 .. 26058365 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAATGGTCGAGCTCCAACTGCCACAATTATTGAATGTAAAAAGAAATTTCACAATCAAATCGCGTGTAGTATAGTCCTAAATCAGCACTATATATCTCAAAAATCAGAATGGGAGTAGCAACGAACTAGCAAGCCAGTGCAATTCCCAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGTGCGAAGAATTACCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGACATTTCTACATCAAAAACCATGGCGTTTCCAAGGAGCTGTACTGGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGGCAAAGGAAAGGAAATTGAAAGTAGGAGTTTCTTGGTACACTCCTAGATTTAAATTGTCGCCTTACGCTGAAAGCTTCAAGTTTTTGGGACCGATTTTTTCAGATTCTGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCACCGCGTCACTCAATTCAGGTCTTCCTAAACTCTATTTCAATCTCAAATCATCCTAAACTAAATGAAAGTATATAAAAACTTAATGGAACGTTTCAAAATACATGGAAATGGTTTTTCTTTATTTTAATTAAGAATGGTTTGATTTTCTTTATTTCTTAAGAAACTAATGTCTTTGGCAAGTTTGCCAAATTTGTTTGCAGGAATCTACTTGATGAATATGGAAGTATAATGATAGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGATGATGGGAGATAGTTTCGAGGACAAATTTTACGAATCTGAATTCAGTAACTGCAATGGCTACATAAGAATAAACAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCGTTTGGAAAGCACACAGACATTAGTTGCGTGACGATATTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTCGATGTAAGACCTCTAGAGGATGCCCTTTTGGTGAACATTGGGGATTTTTTACAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAACAAAATGCGAAGAGATTCACTTTGGGTTTCTTTTTGGTATTTGGGGATGATGATAGGGAACTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATAGAAGAGTTTATAAGCCATTTTCTACCAAAGAATATAGGGCATTTAGGGAAAATAATTATGGGAAAGTTGTTGGAGTACCGATGAGAGAATTTGCTGGAATTGATTTAGAGTGAAGTGGTGAAACATCGATATTACAAATGAAAAAATAGTATCATTTGAGAGGTGTTCGTGGCTCGTCCGGGCCATTCTCCTATGTTATAAAAATATATACTTTAGTTTTGAAACTTCAATGTGCATATCTCAATTCATAAACTTGGAACCAAAAATAACTATGTT

mRNA sequence

GTAATGGTCGAGCTCCAACTGCCACAATTATTGAATGTAAAAAGAAATTTCACAATCAAATCGCGTGTAGTATAGTCCTAAATCAGCACTATATATCTCAAAAATCAGAATGGGAGTAGCAACGAACTAGCAAGCCAGTGCAATTCCCAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGTGCGAAGAATTACCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGACATTTCTACATCAAAAACCATGGCGTTTCCAAGGAGCTGTACTGGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGGCAAAGGAAAGGAAATTGAAAGTAGGAGTTTCTTGGTACACTCCTAGATTTAAATTGTCGCCTTACGCTGAAAGCTTCAAGTTTTTGGGACCGATTTTTTCAGATTCTGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCACCGCGTCACTCAATTCAGGAATCTACTTGATGAATATGGAAGTATAATGATAGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGATGATGGGAGATAGTTTCGAGGACAAATTTTACGAATCTGAATTCAGTAACTGCAATGGCTACATAAGAATAAACAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCGTTTGGAAAGCACACAGACATTAGTTGCGTGACGATATTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTCGATGTAAGACCTCTAGAGGATGCCCTTTTGGTGAACATTGGGGATTTTTTACAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAACAAAATGCGAAGAGATTCACTTTGGGTTTCTTTTTGGTATTTGGGGATGATGATAGGGAACTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATAGAAGAGTTTATAAGCCATTTTCTACCAAAGAATATAGGGCATTTAGGGAAAATAATTATGGGAAAGTTGTTGGAGTACCGATGAGAGAATTTGCTGGAATTGATTTAGAGTGAAGTGGTGAAACATCGATATTACAAATGAAAAAATAGTATCATTTGAGAGGTGTTCGTGGCTCGTCCGGGCCATTCTCCTATGTTATAAAAATATATACTTTAGTTTTGAAACTTCAATGTGCATATCTCAATTCATAAACTTGGAACCAAAAATAACTATGTT

Coding sequence (CDS)

ATGTCAAAAGAATCGATTGAGATGTGCGAAGAATTACCAGTAATGGACATGAATTGTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGACATTTCTACATCAAAAACCATGGCGTTTCCAAGGAGCTGTACTGGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGGCAAAGGAAAGGAAATTGAAAGTAGGAGTTTCTTGGTACACTCCTAGATTTAAATTGTCGCCTTACGCTGAAAGCTTCAAGTTTTTGGGACCGATTTTTTCAGATTCTGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCACCGCGTCACTCAATTCAGGAATCTACTTGATGAATATGGAAGTATAATGATAGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGATGATGGGAGATAGTTTCGAGGACAAATTTTACGAATCTGAATTCAGTAACTGCAATGGCTACATAAGAATAAACAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCGTTTGGAAAGCACACAGACATTAGTTGCGTGACGATATTATTCCAAGACGAGATCGGAGGTATTCAAATGAAATCGAAACAAGGAGATGAGTGGGTCGATGTAAGACCTCTAGAGGATGCCCTTTTGGTGAACATTGGGGATTTTTTACAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAACAAAATGCGAAGAGATTCACTTTGGGTTTCTTTTTGGTATTTGGGGATGATGATAGGGAACTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATAGAAGAGTTTATAAGCCATTTTCTACCAAAGAATATAGGGCATTTAGGGAAAATAATTATGGGAAAGTTGTTGGAGTACCGATGAGAGAATTTGCTGGAATTGATTTAGAGTGA

Protein sequence

MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENNYGKVVGVPMREFAGIDLE
Homology
[Close]
BLAST of MELO3C032832.2 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo])

HSP 1 Score: 664.8 bits (1714), Expect = 1.4e-187
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0 

Query: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60
           MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120
           DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKVVGVPMREFAGIDLE 323
           RENNYGKVVGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]
BLAST of MELO3C032832.2 vs. NCBI nr
Match: XP_004146911.1 (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_4G022900 [Cucumis sativus])

HSP 1 Score: 597.8 bits (1540), Expect = 2.2e-167
Identity = 290/322 (90.06%), Postives = 302/322 (93.79%), Query Frame = 0 

Query: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYGKVVGVPMREFAGIDLE 323
           RENNY K+VGVP+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]
BLAST of MELO3C032832.2 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 387.1 bits (993), Expect = 5.8e-104
Identity = 198/317 (62.46%), Postives = 245/317 (77.29%), Query Frame = 0 

Query: 7   EMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 66
           + C ELPV+D++  + LSES   SL  ACNEWG FYI NHGVS E Y KL ++ DEL   
Sbjct: 5   QTCVELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-R 64

Query: 67  VPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRN 126
           +P++    K+KVG+S Y PRF  SP+ ESFK  GP FS SA  L  TE + GH  TQF N
Sbjct: 65  LPQDT---KIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLL--TEALLGHHNTQFSN 124

Query: 127 LLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIEA 186
           LL+EYGS M +LS+RI+KLLLK++GD+FE+KFY+ EF NC+GY+RIN Y+PR+SNEE+E 
Sbjct: 125 LLEEYGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEG 184

Query: 187 FGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSA 246
            GKHTDISCVTILFQD++GG+QM+SK G EW+D+RP E+AL+VNIGDF+QAWSNERLRSA
Sbjct: 185 LGKHTDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 244

Query: 247 EHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFR----E 306
           EHRVVL+QN KRF++ FF  + DD+ E+YAPSEVVGEGN R+Y+ FSTKEYR FR     
Sbjct: 245 EHRVVLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGAR 304

Query: 307 NNYGKVVGVPMREFAGI 320
             Y K VG  ++EFAGI
Sbjct: 305 GTYEK-VGANVKEFAGI 310

[Close]
BLAST of MELO3C032832.2 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 380.2 bits (975), Expect = 7.1e-102
Identity = 199/322 (61.80%), Postives = 243/322 (75.47%), Query Frame = 0 

Query: 7   EMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 66
           + C ELPV+D+   E LSES+  SL  ACNEWG FYI+NHGVSKE Y +L ++ DEL   
Sbjct: 5   QTCVELPVIDV--YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF-R 64

Query: 67  VPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRN 126
           +P+E K    KVG+S Y PRF  SP+ ESFKF GP FS SA  L  TE +F H  T+F N
Sbjct: 65  LPQETKN---KVGISCYVPRFMASPFFESFKFAGPDFSASAYSL--TEALFDHPNTEFSN 124

Query: 127 LLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSN----- 186
           LL+EYGS M  LS+RI++LLLK++ D FE+KFY+SEFSNC G + IN Y+PR+SN     
Sbjct: 125 LLEEYGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKV 184

Query: 187 EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 246
           EEIE  GKHTDISCVTILFQD +GG+QM+SK G EW+D++P EDAL+VNIGDF+QAWSNE
Sbjct: 185 EEIEGLGKHTDISCVTILFQDNVGGLQMRSK-GGEWMDIKPCEDALVVNIGDFMQAWSNE 244

Query: 247 RLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFR 306
           RLRSAEHRVVL+QN KRF++ FF  +  DDRE+YAPSEVVGEGN R+Y+PFS KEY+ FR
Sbjct: 245 RLRSAEHRVVLRQNVKRFSMSFFWSY-VDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFR 304

Query: 307 ----ENNYGKVVGVPMREFAGI 320
                 NY K VG  +++FAGI
Sbjct: 305 ATSARGNYEK-VGATVKDFAGI 315

[Close]
BLAST of MELO3C032832.2 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 371.7 bits (953), Expect = 2.5e-99
Identity = 197/328 (60.06%), Postives = 245/328 (74.70%), Query Frame = 0 

Query: 4   ESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDEL 63
           ES     ELPV+D+   E LSES+  SL +AC+EWG FYI+NHGVSKE Y KL ++ D+L
Sbjct: 3   ESKSYTVELPVIDV--YEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 62

Query: 64  LTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQ 123
              +P E K    KVG S Y PRF  SP+ ESFKF GP FS SA  L  TE + G    +
Sbjct: 63  F-CLPHETKN---KVGFSCYIPRFIASPFYESFKFSGPDFSASAHHL--TETLLGKHDAE 122

Query: 124 FRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSN-- 183
           F NLL EYGS M ++SRRI+KLLLK++GD+FE+KFY+SEFSNC+GY+R+N Y+P++SN  
Sbjct: 123 FSNLLKEYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNEN 182

Query: 184 ---EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAW 243
              EE+E  GKHTDISCVTILFQD +GG+QM+SK+G EW+D++P EDAL+VNIGDF+ AW
Sbjct: 183 VKIEEVEGLGKHTDISCVTILFQDHVGGLQMRSKEG-EWMDIKPCEDALVVNIGDFMHAW 242

Query: 244 SNERLRSAEHRVVLKQNA-KRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEY 303
           SNE+LRSAEHRVVL+QN+ KRF++ F   F +DD+E+YAPSEVVGEGN R+YKPF TKEY
Sbjct: 243 SNEKLRSAEHRVVLRQNSVKRFSMVFIWSF-EDDKEIYAPSEVVGEGNARLYKPFLTKEY 302

Query: 304 RAFR------ENNYGKVVGVPMREFAGI 320
           R FR        NY K VG  + +FAGI
Sbjct: 303 RIFRATPRGARENYEK-VGANVTDFAGI 319

[Close]
BLAST of MELO3C032832.2 vs. TAIR10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 280.4 bits (716), Expect = 1.4e-75
Identity = 145/322 (45.03%), Postives = 212/322 (65.84%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEE 70
           +LPV+D+   + +  S   SL +AC EWG FY+ NHG+SKE++ K+ +++ ++  A    
Sbjct: 9   QLPVLDLT--QPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA---- 68

Query: 71  AKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSA---SDLGFTEQVFGHRVTQFRNL 130
             E KLK+G   YTPR+  SPY ES    GP FSDSA   +D+ F +    H   + R  
Sbjct: 69  PLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQD----HHKPELRET 128

Query: 131 LDEYGSIMIELSRRIMKLLLKM-MGDSFEDKFYESEFSNCNGYIRINRYAP----RNSNE 190
           + EYG+ M ELS+R++K+LL M +GD    + Y+++FSNC+GY+R+  Y P        E
Sbjct: 129 MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 188

Query: 191 EIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNER 250
            +E  G HTD+SC+TI++QD +GG+QM+SK+G +W+D+ P  D L+VNIGD +QAWSN R
Sbjct: 189 LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEG-KWIDINPCNDFLVVNIGDLMQAWSNGR 248

Query: 251 LRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRE 310
           LRS+EHRVVL++   R +L FFL F +D++ + AP E+VGEG +R YK F   EY  FR+
Sbjct: 249 LRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQ 308

Query: 311 NN-YGKV--VGVPMREFAGIDL 322
           +N  GK   +G  +++FAG+ L
Sbjct: 309 SNEEGKFEKIGFTVKDFAGLKL 318

[Close]
BLAST of MELO3C032832.2 vs. TAIR10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 261.2 bits (666), Expect = 8.7e-70
Identity = 150/333 (45.05%), Postives = 204/333 (61.26%), Query Frame = 0 

Query: 8   MCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAV 67
           M  ELPV D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVT--QFR 127
            E   E K+K+G S YTPRF  SP+ ES +  GP F  SA     +   F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFS 120

Query: 128 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L   GD    K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++++ G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQN---AKRFTLGFFLVFGDDDRELYAPSEVVG--EGNRRVYK 307
            AW+N RLRS++HRV+LK+      RF+L FF  F DD + ++AP EVVG  EG  RV++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAFRENN-YGKV--VGVPMREFAGID 321
            F   +Y  FRE+N  GK   VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]
BLAST of MELO3C032832.2 vs. TAIR10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 128.6 bits (322), Expect = 6.7e-30
Identity = 83/317 (26.18%), Postives = 148/317 (46.69%), Query Frame = 0 

Query: 10  EELPVMDMNCCE---HLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 69
           +E+P +D++  E   H   +    + +AC  WG F + NHG+   L  ++     E    
Sbjct: 30  DEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 89

Query: 70  VPEEAKERKLK------VGVSWYTPRFKLSPYAESFKFLGPIFSDSA----------SDL 129
             EE  +RK+K      +G         +  + E F F      DS           ++L
Sbjct: 90  TTEE--KRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDF---FLQDSTIVPASPEPEDTEL 149

Query: 130 GFTEQVFGHRVTQFRNLLDEYGSIMIELSRRIMKLL---LKMMGDSFEDKFYESEFSNCN 189
                 +    + FR +  EY   + +L+ R+++L+   L + GD     F E       
Sbjct: 150 RKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQ-----T 209

Query: 190 GYIRINRYAPRNSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDAL 249
            ++R N Y P  + E     G+H D   +T+L QD +GG+Q+  +   +W+ V+P+ DAL
Sbjct: 210 SFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDAL 269

Query: 250 LVNIGDFLQAWSNERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRR 305
           ++N+G+ +Q W+N+   SAEHRVV+  + +RF++ FF  F   +  +    E++ E N  
Sbjct: 270 IINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFF-FPSHEANIEPLEELISEENPP 329

[Close]
BLAST of MELO3C032832.2 vs. TAIR10
Match: AT4G21200.1 (gibberellin 2-oxidase 8)

HSP 1 Score: 125.6 bits (314), Expect = 5.7e-29
Identity = 85/314 (27.07%), Postives = 150/314 (47.77%), Query Frame = 0 

Query: 11  ELPVMD----MNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELL-T 70
           ELPV+D    ++  E   E  + ++ +A  EWG F + NHG+S ++  K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEEAKERKLKVG-VSWYTP---RFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRV 130
              +++K  K   G   W TP     +   ++E+F       SD+               
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKD------------F 160

Query: 131 TQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSN 190
           T   + ++++ S    L+  + ++L +  G +    F++        Y+R+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TIL+QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFR 310
             +S EHRV+     +RF+  +F+    D       + +    +R  Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENN---YGKVVGVP 313
           + +   +G  VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]
BLAST of MELO3C032832.2 vs. TAIR10
Match: AT1G50960.1 (gibberellin 2-oxidase 7)

HSP 1 Score: 121.7 bits (304), Expect = 8.2e-28
Identity = 86/305 (28.20%), Postives = 146/305 (47.87%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYK----ACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 70
           +LPV+D++      E  R    K    A  EWG F I NHG+ K+++ ++  + ++ L  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVF-EMMLLEEKKLFD 98

Query: 71  VPEEAKERK-----LKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRV 130
            P   K R+      K    W  P    SP   S      I     S           R+
Sbjct: 99  QPFSVKVRERFSDLSKNSYRWGNPS-ATSPAQYSVSEAFHIILSEVS-----------RI 158

Query: 131 TQFRNLLDEYGSIMIELSRRIMKLLLKMMGD--SFEDKFYESEFSNCNGYIRINRYAPRN 190
           +  RN L       ++   R+ +++ +++G   +   +++E+ F   N ++R+N+Y P  
Sbjct: 159 SDDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSV 218

Query: 191 SNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWS 250
              E+     HTD S +TIL QD+IGG+++++    +W+ V+P  +AL VNIGD  QA S
Sbjct: 219 FGSEVFGLVPHTDTSFLTILSQDQIGGLELEN--NGQWISVKPCLEALTVNIGDMFQALS 278

Query: 251 NERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRA 305
           N   +S  HRV+   N +R ++ FF+         Y  +E+   G  + Y+ FS +EY+ 
Sbjct: 279 NGVYQSVRHRVISPANIERMSIAFFVC-------PYLETEIDCFGYPKKYRRFSFREYKE 321

[Close]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match: sp|F4KBY0|GAOXL_ARATH (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.6e-68
Identity = 150/333 (45.05%), Postives = 204/333 (61.26%), Query Frame = 0 

Query: 8   MCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAV 67
           M  ELPV D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVT--QFR 127
            E   E K+K+G S YTPRF  SP+ ES +  GP F  SA     +   F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFS 120

Query: 128 NLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L   GD    K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++++ G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQN---AKRFTLGFFLVFGDDDRELYAPSEVVG--EGNRRVYK 307
            AW+N RLRS++HRV+LK+      RF+L FF  F DD + ++AP EVVG  EG  RV++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAFRENN-YGKV--VGVPMREFAGID 321
            F   +Y  FRE+N  GK   VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match: sp|O49561|G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 85/314 (27.07%), Postives = 150/314 (47.77%), Query Frame = 0 

Query: 11  ELPVMD----MNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELL-T 70
           ELPV+D    ++  E   E  + ++ +A  EWG F + NHG+S ++  K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEEAKERKLKVG-VSWYTP---RFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRV 130
              +++K  K   G   W TP     +   ++E+F       SD+               
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKD------------F 160

Query: 131 TQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSN 190
           T   + ++++ S    L+  + ++L +  G +    F++        Y+R+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TIL+QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFR 310
             +S EHRV+     +RF+  +F+    D       + +    +R  Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENN---YGKVVGVP 313
           + +   +G  VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match: sp|Q7XP65|G2OX6_ORYSJ (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 8.7e-27
Identity = 91/318 (28.62%), Postives = 145/318 (45.60%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRL----SLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 70
           +LP++D+          R     ++ +A +EWG F + NHGV +EL  ++R     L   
Sbjct: 49  DLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLF-R 108

Query: 71  VPEEAKERK--LKVGVSWYTP-RFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQ 130
           +P E KE+   L     W  P    L   + S  F  P+ S S +D  F +      +T 
Sbjct: 109 LPFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGD------LTS 168

Query: 131 FRNLLDEYGSIMIELSRRIMKLLLKMM-----GDSFEDKFYESEFSNCNGYIRINRYAPR 190
            R ++ E    M  ++  +   L + +     G +    ++ +       ++R+NRY   
Sbjct: 169 LRGVMQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPAC 228

Query: 191 NSNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAW 250
               +      HTD   +T+L QD++GG+ +   +   WV VRP  DAL+VNIGD  QAW
Sbjct: 229 PFAADTFGLVPHTDSDFLTVLCQDQVGGLHL--MKDSRWVAVRPRPDALVVNIGDLFQAW 288

Query: 251 SNERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGE-GNRRVYKPFSTKEY 310
           SN R +S EH+VV      R ++ +FL    D         +VG  G    Y+ F+  EY
Sbjct: 289 SNNRYKSVEHKVVANAKTDRLSVAYFLCPSYD--------SLVGTCGEPSPYRAFTFGEY 348

Query: 311 RAFRENNY---GKVVGVP 313
           R   + +    GK +G+P
Sbjct: 349 RKKVQEDVRTTGKKIGLP 349

[Close]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match: sp|Q9C6I4|G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.5e-26
Identity = 86/305 (28.20%), Postives = 146/305 (47.87%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYK----ACNEWGHFYIKNHGVSKELYWKLRAVTDELLTA 70
           +LPV+D++      E  R    K    A  EWG F I NHG+ K+++ ++  + ++ L  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVF-EMMLLEEKKLFD 98

Query: 71  VPEEAKERK-----LKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRV 130
            P   K R+      K    W  P    SP   S      I     S           R+
Sbjct: 99  QPFSVKVRERFSDLSKNSYRWGNPS-ATSPAQYSVSEAFHIILSEVS-----------RI 158

Query: 131 TQFRNLLDEYGSIMIELSRRIMKLLLKMMGD--SFEDKFYESEFSNCNGYIRINRYAPRN 190
           +  RN L       ++   R+ +++ +++G   +   +++E+ F   N ++R+N+Y P  
Sbjct: 159 SDDRNNLRTIVETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSV 218

Query: 191 SNEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWS 250
              E+     HTD S +TIL QD+IGG+++++    +W+ V+P  +AL VNIGD  QA S
Sbjct: 219 FGSEVFGLVPHTDTSFLTILSQDQIGGLELEN--NGQWISVKPCLEALTVNIGDMFQALS 278

Query: 251 NERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRA 305
           N   +S  HRV+   N +R ++ FF+         Y  +E+   G  + Y+ FS +EY+ 
Sbjct: 279 NGVYQSVRHRVISPANIERMSIAFFVC-------PYLETEIDCFGYPKKYRRFSFREYKE 321

[Close]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match: sp|Q9C899|F6H2_ARATH (Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-26
Identity = 95/314 (30.25%), Postives = 147/314 (46.82%), Query Frame = 0 

Query: 10  EELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPE 69
           E +PV+D++  +  S S   ++  A  EWG F + NHGVS E+   ++  T        E
Sbjct: 60  ESIPVIDISNLDEKSVSK--AVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVE 119

Query: 70  E----AKERKLKVGVSWYTPRFKLSPYAESF----KFLGPIFSDSASDLGFTEQVFGHRV 129
           E    ++E+ L   V + T     SP+AE       +L   F   A              
Sbjct: 120 EKRKFSREKSLSTNVRFGT---SFSPHAEKALEWKDYLSLFFVSEAEASQLWPDSCRSET 179

Query: 130 TQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFE----DKFYESEFSNCNGYIRIN-RYA 189
            ++ N            ++ ++K LL+ +G++      DK  ES F    G  RIN  Y 
Sbjct: 180 LEYMN-----------ETKPLVKKLLRFLGENLNVKELDKTKESFFM---GSTRINLNYY 239

Query: 190 PRNSNEEIE-AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFL 249
           P   N E+    G+H+D+S +TIL QDEIGG+ ++S     WV V P+  +L++NIGD +
Sbjct: 240 PICPNPELTVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLVINIGDAM 299

Query: 250 QAWSNERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTK 308
           Q  SN R +S EHRV+   +  R ++  F V    +  +    EV+  G + VYK     
Sbjct: 300 QIMSNGRYKSVEHRVLANGSYNRISVPIF-VSPKPESVIGPLLEVIENGEKPVYKDILYT 353

[Close]
BLAST of MELO3C032832.2 vs. TrEMBL
Match: tr|A0A1S3BYJ4|A0A1S3BYJ4_CUCME (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 9.5e-188
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0 

Query: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60
           MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120
           DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180
           VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYGKVVGVPMREFAGIDLE 323
           RENNYGKVVGVPMREFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]
BLAST of MELO3C032832.2 vs. TrEMBL
Match: tr|A0A0A0KW97|A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.4e-167
Identity = 290/322 (90.06%), Postives = 302/322 (93.79%), Query Frame = 0 

Query: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYGKVVGVPMREFAGIDLE 323
           RENNY K+VGVP+REFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]
BLAST of MELO3C032832.2 vs. TrEMBL
Match: tr|A0A2P4JT81|A0A2P4JT81_QUESU (Gibberellin 20-oxidase-like protein OS=Quercus suber OX=58331 GN=CFP56_37011 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.8e-77
Identity = 161/317 (50.79%), Postives = 215/317 (67.82%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEE 70
           ELP++D++  + L+ S   SL +AC +WG F+I NHG+SK+L+ KL  ++++L       
Sbjct: 9   ELPILDIS--QPLNPSFLSSLAEACKKWGFFHIINHGISKDLFSKLYLLSNDLFNL---- 68

Query: 71  AKERKLKVG----VSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRN 130
             + KLKVG    +  YTP F  SPY ES +  GP F DSA        +F  + ++F  
Sbjct: 69  PSDTKLKVGPFSSIKTYTPHFIASPYFESLRVSGPNFLDSAQ--SSANALFVQQNSEFSE 128

Query: 131 LLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNE-EIE 190
           +L EYG  M ELS+ I+K+LL  + D F  K YESEF NC+GY+RIN Y+P  S E E+E
Sbjct: 129 ILQEYGRKMEELSKNIIKVLLMSLKDGFGKKDYESEFKNCHGYMRINNYSPPESLEVEVE 188

Query: 191 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDEIGG+Q+KSK+G +W+D+RP E  L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCVTIVYQDEIGGLQVKSKEG-KWMDIRPCEGTLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENN- 310
           +EHRVVLKQ A RF+L FF  F +D++ + AP EVVGEGN R+Y PF   +Y  FRE+N 
Sbjct: 249 SEHRVVLKQPANRFSLAFFWCF-EDEKVIIAPDEVVGEGNVRLYNPFVCLDYLKFRESNE 308

Query: 311 YGKV--VGVPMREFAGI 320
            GK   VG  +R+FAGI
Sbjct: 309 RGKFEKVGFTVRDFAGI 315

[Close]
BLAST of MELO3C032832.2 vs. TrEMBL
Match: tr|B9RDS1|B9RDS1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_1615250 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.0e-77
Identity = 153/319 (47.96%), Postives = 222/319 (69.59%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEE 70
           ELP++D++  + +++SS  SL +AC EWG F I NHG+SK+LY K+  ++  +  ++P E
Sbjct: 9   ELPILDIS--KPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIF-SLPSE 68

Query: 71  AKERKLKVG----VSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRN 130
               K K+G    +  YTP F  SPY ES +  GP F  SA      + +F  + ++F +
Sbjct: 69  I---KFKLGPSSSIKTYTPHFIASPYFESLRVSGPNFLASAQ--SSADVLFNQQNSEFSD 128

Query: 131 LLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYA-PRNSNEEIE 190
           +L EYGS M ELS+RI+++LL  +GD  + KFYE+EF+NC+GY+RI  Y  P+N  +E+E
Sbjct: 129 VLQEYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLEDEVE 188

Query: 191 AFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SC+TI++QDEIGG+Q+KS++G +W+D+ P E+ L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCITIVYQDEIGGLQVKSREG-KWMDISPCEETLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENN- 310
           +EH+VVLKQ+A R +L FF  F +D + + AP EVVGEG  R+YKPF   +Y  FRE+N 
Sbjct: 249 SEHQVVLKQSANRLSLAFFWCF-EDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 308

Query: 311 YGKV--VGVPMREFAGIDL 322
            GK   VG  +++FAG++L
Sbjct: 309 RGKFEKVGFTVKDFAGLNL 317

[Close]
BLAST of MELO3C032832.2 vs. TrEMBL
Match: tr|E0CV03|E0CV03_VITVI (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0050g00640 PE=3 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 6.8e-77
Identity = 154/317 (48.58%), Postives = 216/317 (68.14%), Query Frame = 0 

Query: 11  ELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVTDELLTAVPEE 70
           ELPV D+   +    SS  SL  AC EWG F+I NHG+S +L+ KL+++++ L   +P  
Sbjct: 9   ELPVFDIGQSQPFDPSSLSSLLLACKEWGFFHITNHGISMDLFKKLKSLSNSLF-CLPSV 68

Query: 71  AKERKLKVGVS----WYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHRVTQFRN 130
           +   KLKVG S     YTP F  SP+ ESF+  GP F  SA      E VF H  ++F  
Sbjct: 69  S---KLKVGPSSSLRTYTPHFIASPFFESFRVSGPDFFTSAQ--SSAEVVFNHPNSEFSE 128

Query: 131 LLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNSNEEIEA 190
           +  EY + M +LS+R+++++L  +G+ FE + YESEF+NCNGY+RIN Y+P  S +E+E 
Sbjct: 129 VFQEYCTKMSQLSKRMIEVVLMCLGNDFEKRLYESEFNNCNGYLRINNYSPPESTKEVEG 188

Query: 191 FGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSA 250
            G HTD+SC+TI++QDEIGG+Q++SK+G +W+D+ P E  L+VNIGD +QAWSN  LRS+
Sbjct: 189 LGMHTDMSCITIVYQDEIGGLQVRSKEG-KWMDINPCEGTLVVNIGDLMQAWSNANLRSS 248

Query: 251 EHRVVLK-QNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAFRENN- 310
           EHRVVLK Q+  R +L FF  F +DD+ + +P+EVVGEGN R+Y+PF   +Y  FRE+N 
Sbjct: 249 EHRVVLKQQHVNRLSLAFFWCF-EDDKVIISPNEVVGEGNSRIYQPFVCADYLKFRESNE 308

Query: 311 YGKV--VGVPMREFAGI 320
            GK   VG  +++FAGI
Sbjct: 309 KGKFEKVGFTVKDFAGI 317

The following BLAST results are available for this feature:
BLAST of MELO3C032832.2 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008453897.11.4e-187100.00PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo][more]
XP_004146911.12.2e-16790.06PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus] >KGN53154.1 hypotheti... [more]
XP_022141254.15.8e-10462.46gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia][more]
XP_022141253.17.1e-10261.80gibberellin 20-oxidase-like protein [Momordica charantia][more]
XP_022140867.12.5e-9960.06gibberellin 20-oxidase-like protein [Momordica charantia][more]
BLAST of MELO3C032832.2 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G23340.11.4e-7545.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT5G51310.18.7e-7045.052-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT3G19000.16.7e-3026.182-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein[more]
AT4G21200.15.7e-2927.07gibberellin 2-oxidase 8[more]
AT1G50960.18.2e-2828.20gibberellin 2-oxidase 7[more]
BLAST of MELO3C032832.2 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|F4KBY0|GAOXL_ARATH1.6e-6845.05Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310... [more]
sp|O49561|G2OX8_ARATH1.0e-2727.07Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ... [more]
sp|Q7XP65|G2OX6_ORYSJ8.7e-2728.62Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2... [more]
sp|Q9C6I4|G2OX7_ARATH1.5e-2628.20Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ... [more]
sp|Q9C899|F6H2_ARATH2.5e-2630.25Feruloyl CoA ortho-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=F6'H2 PE=1 S... [more]
BLAST of MELO3C032832.2 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A1S3BYJ4|A0A1S3BYJ4_CUCME9.5e-188100.00gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV... [more]
tr|A0A0A0KW97|A0A0A0KW97_CUCSA1.4e-16790.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1[more]
tr|A0A2P4JT81|A0A2P4JT81_QUESU1.8e-7750.79Gibberellin 20-oxidase-like protein OS=Quercus suber OX=58331 GN=CFP56_37011 PE=... [more]
tr|B9RDS1|B9RDS1_RICCO4.0e-7747.96Gibberellin 20-oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_1615250 PE=... [more]
tr|E0CV03|E0CV03_VITVI6.8e-7748.58Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_16s0050g00640 PE=3 SV=... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
Vocabulary: INTERPRO
TermDefinition
IPR026992DIOX_N
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR027443IPNS-like
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MELO3C032832.2.1MELO3C032832.2.1mRNA


InterPro
Analysis Name: InterPro Annotations of melon v3.6.1
Date Performed: 2018-09-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 9..311
e-value: 1.1E-60
score: 207.6
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 171..264
e-value: 1.2E-19
score: 70.5
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 166..267
score: 10.727
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 12..74
e-value: 9.1E-9
score: 36.0
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 8..308
NoneNo IPR availablePANTHERPTHR10209:SF212OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 8..308
NoneNo IPR availableSUPERFAMILYSSF51197Clavaminate synthase-likecoord: 10..305