Cucsa.094210 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.094210
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Locationscaffold00919 : 415888 .. 417262 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCCAGTTTAATTCCTAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGTCATCCTAAACTCTATTTCAATCTCAAATCCTCCCGGATTTAATTATTATGATAAAATTACAAATTTGATTAAAGCTTAAGAATCGTTCTATTTAAAATATCTTCGGCAAGTTAAATTTGTTTGCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGTATGGACGTTTGGAATGAAAATAGGTATTCATTCTTCTCGTATTAGGATTGGTTGCTGGCTCATGCATAATGCATACATATATGTTATAGTTTTTAAACTTTCAATATGCATATCTCAATTTATGAACTTGGAACAAAAGACAACTATGATGATCTCTTGATGACTTACACATGTACAAGTATAAGTGTATGTAAGATAAAGTTATGTTGGGTTTGCTATACTCGAATCT

mRNA sequence

AAGCCAGTTTAATTCCTAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGTATGGACGTTTGGAATGAAAATAGGTATTCATTCTTCTCGTATTAGGATTGGTTGCTGGCTCATGCATAATGCATACATATATGTTATAGTTTTTAAACTTTCAATATGCATATCTCAATTTATGAACTTGGAACAAAAGACAACTATGATGATCTCTTGATGACTTACACATGTACAAGTATAAGTGTATGTAAGATAAAGTTATGTTGGGTTTGCTATACTCGAATCT

Coding sequence (CDS)

ATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGA

Protein sequence

MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYRKIVGVPLREFAGIDLE*
Homology
[Close]
BLAST of Cucsa.094210 vs. Swiss-Prot
Match: GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.9e-28
Identity = 97/318 (30.50%), Postives = 158/318 (49.69%), Query Frame = 1 

Query: 3   KESIEMHE-ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTD 62
           K SI++ E  +  +D++S +   E+ R+ + +AC + G F + NHGVS+ L      + +
Sbjct: 53  KPSIDIPELNVPFIDLSSQDSTLEAPRV-IAEACTKHGFFLVVNHGVSESLIADAHRLME 112

Query: 63  ELLTAVPEETKEGKLKVG-----ASWYTPRFRLS-PYIE--SFKFLGPN-----FSDYAS 122
                     ++ + K G     AS +T RF    P+ E  SF+F   N       DY S
Sbjct: 113 SFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFS 172

Query: 123 DLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNG 182
           D        GQ   QF K+  +Y   M  LS +IM+LL  ++G N +  ++   F   + 
Sbjct: 173 D------TLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD--YFRGFFEENDS 232

Query: 183 YLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL 242
            +R+N Y P  + +     G H D S +TI+ QD + G+Q+     ++W  +RP   A +
Sbjct: 233 IMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVF--VDNQWQSIRPNPKAFV 292

Query: 243 VNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRI 302
           VNIGD   A SN   +S  HR V+ ++  R S+AFFL  K  D+ +  PS+++ +  TR 
Sbjct: 293 VNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK-KDKVVKPPSDILEKMKTRK 352

Query: 303 YKPFSTKEYRAYRENNYR 307
           Y  F+   +  + + +YR
Sbjct: 353 YPDFTWSMFLEFTQKHYR 358

[Close]
BLAST of Cucsa.094210 vs. Swiss-Prot
Match: G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 125.2 bits (313), Expect = 1.3e-27
Identity = 88/314 (28.03%), Postives = 151/314 (48.09%), Query Frame = 1 

Query: 11  ELLVMDMN----SCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 70
           EL V+D++      E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  -VPEETKEGKLKVGA-SWYTPR---FRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TI++QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQLI--KDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]
BLAST of Cucsa.094210 vs. Swiss-Prot
Match: GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 1.2e-25
Identity = 90/292 (30.82%), Postives = 141/292 (48.29%), Query Frame = 1 

Query: 25  ESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVG----- 84
           ++SRL + +AC + G F + NHG+S+EL       T         E +    K G     
Sbjct: 78  DASRL-ISEACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGY 137

Query: 85  ASWYTPRFRLS-PYIESFKFLGPNFSDYASDLGFTEQVF----GQRVTQFRKLLDEYGSI 144
           AS +T RF    P+ E+  F    F D  S     +  F    G     F K+  EY   
Sbjct: 138 ASSFTGRFSTKLPWKETLSF---RFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEA 197

Query: 145 MMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDIS 204
           M  LS +IM+LL  ++G  ++  ++   F   +  +R+N Y P    +     G H D +
Sbjct: 198 MSSLSLKIMELLGLSLG--VKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPT 257

Query: 205 CVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQ 264
            +TI+ QD + G+Q+  +  ++W  +RP   A +VNIGD   A SN+R +S  HR V+  
Sbjct: 258 SLTILHQDHVNGLQVFVE--NQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNS 317

Query: 265 DVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 307
           + +R SLAFFL  K  DR +  P E++    +R Y  F+   +  + + +YR
Sbjct: 318 ESERKSLAFFLCPK-KDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYR 360

[Close]
BLAST of Cucsa.094210 vs. Swiss-Prot
Match: G2OX7_ARATH (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-25
Identity = 85/309 (27.51%), Postives = 149/309 (48.22%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYK----ACNEWGHFYIKNHGVSKELYQKL----RAVTDE 70
           +L V+D++      E  R    K    A  EWG F I NHG+ K++++ +    + + D+
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 71  LLTAVPEETKEGKLKVGASWYTPRFRLSP----YIESFKFLGPNFSDYASDLGFTEQVFG 130
             +    E      K    W  P    SP      E+F  +    S  + D         
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPS-ATSPAQYSVSEAFHIILSEVSRISDDRN------- 158

Query: 131 QRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPR 190
                 R +++ Y   +  +++ I ++L K +  N+  +++E+ F   N +LR+N+Y P 
Sbjct: 159 ----NLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPS 218

Query: 191 NSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAW 250
               E+     HTD S +TI+ QD+IGG+++  +   +W+ V+P  +AL VNIGD  QA 
Sbjct: 219 VFGSEVFGLVPHTDTSFLTILSQDQIGGLEL--ENNGQWISVKPCLEALTVNIGDMFQAL 278

Query: 251 SNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYR 308
           SN   +S  HRV+   +++R S+AFF+         Y  +E+   G  + Y+ FS +EY+
Sbjct: 279 SNGVYQSVRHRVISPANIERMSIAFFVC-------PYLETEIDCFGYPKKYRRFSFREYK 324

[Close]
BLAST of Cucsa.094210 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.7e-25
Identity = 86/280 (30.71%), Postives = 129/280 (46.07%), Query Frame = 1 

Query: 33  KACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVG-----ASWYTPRF 92
           +AC   G F + NHG+  EL        D   T    E +    + G     AS +T RF
Sbjct: 64  EACERHGFFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRF 123

Query: 93  RLS-PYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL 152
               P+ E+  F     SD A  + +     G+   +  ++   Y S M  LS  IM++L
Sbjct: 124 ASKLPWKETLSFRSCP-SDPALVVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVL 183

Query: 153 LKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGG 212
            +++G  +    Y   F   +  +R+N Y P     E    G H D + +TI+ QD +GG
Sbjct: 184 GESLG--VGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGG 243

Query: 213 IQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLI 272
           +Q+  +    W  +RP  DA +VNIGD   A SN R +S  HR V+   V R SLAFFL 
Sbjct: 244 LQVHTE--GRWRSIRPRADAFVVNIGDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLC 303

Query: 273 FKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 307
             + D+ +  P  +V   N R Y  F+ +    + + +YR
Sbjct: 304 -PEMDKVVAPPGTLVDAANPRAYPDFTWRSLLDFTQKHYR 337

[Close]
BLAST of Cucsa.094210 vs. TrEMBL
Match: A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 5.4e-185
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 1 

Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNYRKIVGVPLREFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]
BLAST of Cucsa.094210 vs. TrEMBL
Match: B9GQ95_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s16090g PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.2e-77
Identity = 155/319 (48.59%), Postives = 224/319 (70.22%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + L  S+  SL +AC EWG F I NHG+SKELY KL +++ +L   +P E
Sbjct: 8   ELPILDIS--QPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLF-GLPAE 67

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           TK   L++G S     YTP F  SP+ E  +  GPNF  +AS     + +FGQ+  +FR 
Sbjct: 68  TK---LELGPSSSINTYTPHFIASPFFECLRVFGPNF--FASAQSSADTLFGQQSFEFRA 127

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRY-APRNSNEEIE 190
            L EYG+ M ELS+RI+++L  ++G+  ++K+YESEF NC+GYLRI  Y  P+   +E+E
Sbjct: 128 ALQEYGTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVDEVE 187

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDE+GG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSN++LRS
Sbjct: 188 GLGMHTDMSCVTIVYQDEVGGLQVKSREG-KWMDISPGEETLVVNIGDLLQAWSNDKLRS 247

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EHR+VLK+ V R SLAFF  F +D++ + AP+EVVGEGN RIY+PF   +Y  +RE++ 
Sbjct: 248 SEHRIVLKKPVNRLSLAFFWCF-EDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSE 307

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAGI L
Sbjct: 308 RGKFEKVGFTVKDFAGISL 316

[Close]
BLAST of Cucsa.094210 vs. TrEMBL
Match: B9RDS1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-76
Identity = 156/319 (48.90%), Postives = 222/319 (69.59%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + +++SS  SL +AC EWG F I NHG+SK+LY K+  ++  + + +P E
Sbjct: 9   ELPILDIS--KPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIFS-LPSE 68

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
            K    K+G S     YTP F  SPY ES +  GPNF   AS     + +F Q+ ++F  
Sbjct: 69  IK---FKLGPSSSIKTYTPHFIASPYFESLRVSGPNF--LASAQSSADVLFNQQNSEFSD 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYA-PRNSNEEIE 190
           +L EYGS M ELS+RI+++LL ++GD L+ KFYE+EF+NC+GYLRI  Y  P+N  +E+E
Sbjct: 129 VLQEYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLEDEVE 188

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SC+TI++QDEIGG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCITIVYQDEIGGLQVKSREG-KWMDISPCEETLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EH+VVLKQ   R SLAFF  F +D + + AP EVVGEG  RIYKPF   +Y  +RE+N 
Sbjct: 249 SEHQVVLKQSANRLSLAFFWCF-EDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 308

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAG++L
Sbjct: 309 RGKFEKVGFTVKDFAGLNL 317

[Close]
BLAST of Cucsa.094210 vs. TrEMBL
Match: E0CV03_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0050g00640 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 5.9e-75
Identity = 151/317 (47.63%), Postives = 218/317 (68.77%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V D+   +    SS  SL  AC EWG F+I NHG+S +L++KL+++++ L   +P  
Sbjct: 9   ELPVFDIGQSQPFDPSSLSSLLLACKEWGFFHITNHGISMDLFKKLKSLSNSLF-CLPSV 68

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           +K   LKVG S     YTP F  SP+ ESF+  GP+F  + S     E VF    ++F +
Sbjct: 69  SK---LKVGPSSSLRTYTPHFIASPFFESFRVSGPDF--FTSAQSSAEVVFNHPNSEFSE 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEA 190
           +  EY + M +LS+R+++++L  +G++ E + YESEF+NCNGYLRIN Y+P  S +E+E 
Sbjct: 129 VFQEYCTKMSQLSKRMIEVVLMCLGNDFEKRLYESEFNNCNGYLRINNYSPPESTKEVEG 188

Query: 191 FGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSA 250
            G HTD+SC+TI++QDEIGG+Q++ K+G +W+D+ P E  L+VNIGD +QAWSN  LRS+
Sbjct: 189 LGMHTDMSCITIVYQDEIGGLQVRSKEG-KWMDINPCEGTLVVNIGDLMQAWSNANLRSS 248

Query: 251 EHRVVLKQD-VKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           EHRVVLKQ  V R SLAFF  F +DD+ + +P+EVVGEGN+RIY+PF   +Y  +RE+N 
Sbjct: 249 EHRVVLKQQHVNRLSLAFFWCF-EDDKVIISPNEVVGEGNSRIYQPFVCADYLKFRESNE 308

Query: 311 R---KIVGVPLREFAGI 320
           +   + VG  +++FAGI
Sbjct: 309 KGKFEKVGFTVKDFAGI 317

[Close]
BLAST of Cucsa.094210 vs. TrEMBL
Match: A0A067JZJ3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20586 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.0e-74
Identity = 151/320 (47.19%), Postives = 219/320 (68.44%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V+D++    +S  S  S+YKAC EWG F + NHG+S+ L+ ++ + + ++ T     
Sbjct: 9   ELPVLDLSQLVDISSLS--SVYKACQEWGFFSVTNHGISRNLFDEVCSHSKQIFTL---- 68

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
             + KLK+G S     YTP+F  SPY ES +  GP+F  +AS     +++F Q   +F +
Sbjct: 69  PLDFKLKLGPSSCLKTYTPQFIASPYFESLRVSGPDF--FASSKSSADKLFSQESCKFSE 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNE--EI 190
           +L EYG+ M+ELS+RI+ ++L ++GD  + KFYE EFSNCNGYLRI  Y+P  + E  E+
Sbjct: 129 ILQEYGNKMVELSKRIINVVLMSLGDGYDRKFYEMEFSNCNGYLRIVNYSPPKNVEEMEV 188

Query: 191 EAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLR 250
           E  G HTD+SC+TI++QDEIGG+QM+ K+G EW+D+RP E+AL+VNIGD +QAWSN RLR
Sbjct: 189 EGLGMHTDMSCITILYQDEIGGLQMRSKEG-EWMDIRPRENALVVNIGDLMQAWSNGRLR 248

Query: 251 SAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENN 310
           S+EHRV+LK+ V R SLAFF  F +DD+ + AP ++VG+ + R+YKPF   +Y  +RENN
Sbjct: 249 SSEHRVILKRLVNRLSLAFFWCF-EDDKVISAPEDIVGKESLRLYKPFVCLDYLKFRENN 308

Query: 311 YR---KIVGVPLREFAGIDL 322
                  +G  + +FAG+ L
Sbjct: 309 EEGKFDKIGYTVNDFAGVKL 318

[Close]
BLAST of Cucsa.094210 vs. TAIR10
Match: AT4G23340.1 (AT4G23340.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 272.3 bits (695), Expect = 3.8e-73
Identity = 142/326 (43.56%), Postives = 213/326 (65.34%), Query Frame = 1 

Query: 7   EMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 66
           E+H  L +  ++  + +  S   SL +AC EWG FY+ NHG+SKE++ K+ +++ ++  A
Sbjct: 3   ELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA 62

Query: 67  VPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYA---SDLGFTEQVFGQRVTQ 126
                 E KLK+G   YTPR+  SPY ES    GP+FSD A   +D+ F +        +
Sbjct: 63  ----PLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDH----HKPE 122

Query: 127 FRKLLDEYGSIMMELSRRIMKLLLK-TMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNE 186
            R+ + EYG+ M ELS+R++K+LL  T+GD    + Y+++FSNC+GYLR+  Y P +  E
Sbjct: 123 LRETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVE 182

Query: 187 E----IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAW 246
           +    +E  G HTD+SC+TI++QD +GG+QM+ K+G +W+D+ P  D L+VNIGD +QAW
Sbjct: 183 KQEELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEG-KWIDINPCNDFLVVNIGDLMQAW 242

Query: 247 SNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYR 306
           SN RLRS+EHRVVL++ V R SLAFFL F +D++ + AP E+VGEG  R YK F   EY 
Sbjct: 243 SNGRLRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYL 302

Query: 307 AYRENNYR---KIVGVPLREFAGIDL 322
            +R++N     + +G  +++FAG+ L
Sbjct: 303 KFRQSNEEGKFEKIGFTVKDFAGLKL 318

[Close]
BLAST of Cucsa.094210 vs. TAIR10
Match: AT5G51310.1 (AT5G51310.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 255.4 bits (651), Expect = 4.8e-68
Identity = 150/333 (45.05%), Postives = 210/333 (63.06%), Query Frame = 1 

Query: 8   MHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAV 67
           M  EL V D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y+KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVT--QFR 127
            E   E K+K+GAS YTPRF  SP+ ES +  GP+F  YAS     +  F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDF--YASAKSSVD-AFSDQATDEEFS 120

Query: 128 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L + GD+L  K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++ + G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQD---VKRFSLAFFLIFKDDDRELYAPSEVVG--EGNTRIYK 307
            AW+N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAYRENNYR---KIVGVPLREFAGID 321
            F   +Y  +RE+N +   + VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]
BLAST of Cucsa.094210 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 126.3 bits (316), Expect = 3.4e-29
Identity = 97/318 (30.50%), Postives = 158/318 (49.69%), Query Frame = 1 

Query: 3   KESIEMHE-ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTD 62
           K SI++ E  +  +D++S +   E+ R+ + +AC + G F + NHGVS+ L      + +
Sbjct: 53  KPSIDIPELNVPFIDLSSQDSTLEAPRV-IAEACTKHGFFLVVNHGVSESLIADAHRLME 112

Query: 63  ELLTAVPEETKEGKLKVG-----ASWYTPRFRLS-PYIE--SFKFLGPN-----FSDYAS 122
                     ++ + K G     AS +T RF    P+ E  SF+F   N       DY S
Sbjct: 113 SFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFS 172

Query: 123 DLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNG 182
           D        GQ   QF K+  +Y   M  LS +IM+LL  ++G N +  ++   F   + 
Sbjct: 173 D------TLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD--YFRGFFEENDS 232

Query: 183 YLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL 242
            +R+N Y P  + +     G H D S +TI+ QD + G+Q+     ++W  +RP   A +
Sbjct: 233 IMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVF--VDNQWQSIRPNPKAFV 292

Query: 243 VNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRI 302
           VNIGD   A SN   +S  HR V+ ++  R S+AFFL  K  D+ +  PS+++ +  TR 
Sbjct: 293 VNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK-KDKVVKPPSDILEKMKTRK 352

Query: 303 YKPFSTKEYRAYRENNYR 307
           Y  F+   +  + + +YR
Sbjct: 353 YPDFTWSMFLEFTQKHYR 358

[Close]
BLAST of Cucsa.094210 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 125.9 bits (315), Expect = 4.4e-29
Identity = 86/319 (26.96%), Postives = 153/319 (47.96%), Query Frame = 1 

Query: 10  EELLVMDMNSCE---HLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 69
           +E+  +D++S E   H   +    + +AC  WG F + NHG+   L  ++     E    
Sbjct: 30  DEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 89

Query: 70  VPEETKEGKL-KVGASWYTPRFR---LSPYIESFKFL---------GPNFSDYASDLGFT 129
             EE ++ K  +V    Y        +  + E F F           P   D  ++L   
Sbjct: 90  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPED--TELRKL 149

Query: 130 EQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL---LKTMGDNLEDKFYESEFSNCNGYL 189
              + Q  + FR++  EY   + +L+ R+++L+   L   GD L   F E        +L
Sbjct: 150 TNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQ-----TSFL 209

Query: 190 RINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVN 249
           R N Y P  + E     G+H D   +T++ QD +GG+Q+  +   +W+ V+P+ DAL++N
Sbjct: 210 RFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIIN 269

Query: 250 IGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYK 309
           +G+ +Q W+N+   SAEHRVV+    +RFS+ FF  F   +  +    E++ E N   YK
Sbjct: 270 MGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFF-FFPSHEANIEPLEELISEENPPCYK 329

[Close]
BLAST of Cucsa.094210 vs. TAIR10
Match: AT4G21200.1 (AT4G21200.1 gibberellin 2-oxidase 8)

HSP 1 Score: 125.2 bits (313), Expect = 7.5e-29
Identity = 88/314 (28.03%), Postives = 151/314 (48.09%), Query Frame = 1 

Query: 11  ELLVMDMN----SCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 70
           EL V+D++      E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  -VPEETKEGKLKVGA-SWYTPR---FRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TI++QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQLI--KDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]
BLAST of Cucsa.094210 vs. NCBI nr
Match: gi|449458351|ref|XP_004146911.1| (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus])

HSP 1 Score: 654.8 bits (1688), Expect = 7.7e-185
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 1 

Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNYRKIVGVPLREFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]
BLAST of Cucsa.094210 vs. NCBI nr
Match: gi|659107869|ref|XP_008453897.1| (PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo])

HSP 1 Score: 591.3 bits (1523), Expect = 1.0e-165
Identity = 290/322 (90.06%), Postives = 302/322 (93.79%), Query Frame = 1 

Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNY K+VGVP+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]
BLAST of Cucsa.094210 vs. NCBI nr
Match: gi|743828709|ref|XP_011023338.1| (PREDICTED: feruloyl CoA ortho-hydroxylase 2-like [Populus euphratica])

HSP 1 Score: 300.1 bits (767), Expect = 4.8e-78
Identity = 157/319 (49.22%), Postives = 226/319 (70.85%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + L  S+  SL +AC EWG F I NHG+SKELY+KL +++ +L   +P E
Sbjct: 8   ELPILDIS--QPLQPSALASLAEACQEWGFFRISNHGISKELYKKLYSLSQQLF-GLPAE 67

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           TK   L++G S     YTP F  SP+ E  +  GPNF  +AS     + +FGQ+  +FR 
Sbjct: 68  TK---LELGPSSSINTYTPHFIASPFFECLRVFGPNF--FASAQSSADTLFGQQSFEFRA 127

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRY-APRNSNEEIE 190
            L EYG+ M ELS+RI+++LL ++G+  ++K+YESEF NC+GYLRI  Y  P+   +E+E
Sbjct: 128 ALQEYGTKMTELSKRIVEILLMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVDEVE 187

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDE+GG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSNE+LRS
Sbjct: 188 GLGMHTDMSCVTIVYQDEVGGLQVKSREG-KWMDISPSEETLVVNIGDLLQAWSNEKLRS 247

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EHR+VLK+ V R SLAFF  F +D++ + AP+EVVGEGN RIY+PF   +Y  +RE++ 
Sbjct: 248 SEHRIVLKKPVNRLSLAFFWCF-EDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSE 307

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAGI L
Sbjct: 308 RGKFEKVGFTVKDFAGISL 316

[Close]
BLAST of Cucsa.094210 vs. NCBI nr
Match: gi|224067894|ref|XP_002302586.1| (hypothetical protein POPTR_0002s16090g [Populus trichocarpa])

HSP 1 Score: 295.4 bits (755), Expect = 1.2e-76
Identity = 155/319 (48.59%), Postives = 224/319 (70.22%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + L  S+  SL +AC EWG F I NHG+SKELY KL +++ +L   +P E
Sbjct: 8   ELPILDIS--QPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLF-GLPAE 67

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           TK   L++G S     YTP F  SP+ E  +  GPNF  +AS     + +FGQ+  +FR 
Sbjct: 68  TK---LELGPSSSINTYTPHFIASPFFECLRVFGPNF--FASAQSSADTLFGQQSFEFRA 127

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRY-APRNSNEEIE 190
            L EYG+ M ELS+RI+++L  ++G+  ++K+YESEF NC+GYLRI  Y  P+   +E+E
Sbjct: 128 ALQEYGTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVDEVE 187

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDE+GG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSN++LRS
Sbjct: 188 GLGMHTDMSCVTIVYQDEVGGLQVKSREG-KWMDISPGEETLVVNIGDLLQAWSNDKLRS 247

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EHR+VLK+ V R SLAFF  F +D++ + AP+EVVGEGN RIY+PF   +Y  +RE++ 
Sbjct: 248 SEHRIVLKKPVNRLSLAFFWCF-EDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSE 307

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAGI L
Sbjct: 308 RGKFEKVGFTVKDFAGISL 316

[Close]
BLAST of Cucsa.094210 vs. NCBI nr
Match: gi|255541592|ref|XP_002511860.1| (PREDICTED: gibberellin 20 oxidase 1 [Ricinus communis])

HSP 1 Score: 295.0 bits (754), Expect = 1.5e-76
Identity = 156/319 (48.90%), Postives = 222/319 (69.59%), Query Frame = 1 

Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + +++SS  SL +AC EWG F I NHG+SK+LY K+  ++  + + +P E
Sbjct: 9   ELPILDIS--KPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIFS-LPSE 68

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
            K    K+G S     YTP F  SPY ES +  GPNF   AS     + +F Q+ ++F  
Sbjct: 69  IK---FKLGPSSSIKTYTPHFIASPYFESLRVSGPNF--LASAQSSADVLFNQQNSEFSD 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYA-PRNSNEEIE 190
           +L EYGS M ELS+RI+++LL ++GD L+ KFYE+EF+NC+GYLRI  Y  P+N  +E+E
Sbjct: 129 VLQEYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLEDEVE 188

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SC+TI++QDEIGG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCITIVYQDEIGGLQVKSREG-KWMDISPCEETLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EH+VVLKQ   R SLAFF  F +D + + AP EVVGEG  RIYKPF   +Y  +RE+N 
Sbjct: 249 SEHQVVLKQSANRLSLAFFWCF-EDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 308

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAG++L
Sbjct: 309 RGKFEKVGFTVKDFAGLNL 317

The following BLAST results are available for this feature:
BLAST of Cucsa.094210 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GAOX2_ARATH5.9e-2830.50Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana GN=GA20OX2 PE=2 SV=1[more]
G2OX8_ARATH1.3e-2728.03Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana GN=GA2OX8 PE=1 SV=2[more]
GAOX1_ARATH1.2e-2530.82Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2[more]
G2OX7_ARATH2.1e-2527.51Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana GN=GA2OX7 PE=1 SV=1[more]
GAO1B_WHEAT4.7e-2530.71Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1[more]
BLAST of Cucsa.094210 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KW97_CUCSA5.4e-185100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G022900 PE=3 SV=1[more]
B9GQ95_POPTR8.2e-7748.59Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0002s16090g PE=3 SV=1[more]
B9RDS1_RICCO1.1e-7648.90Gibberellin 20-oxidase, putative OS=Ricinus communis GN=RCOM_1615250 PE=3 SV=1[more]
E0CV03_VITVI5.9e-7547.63Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0050g00640 PE=3 SV=... [more]
A0A067JZJ3_JATCU1.0e-7447.19Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20586 PE=3 SV=1[more]
BLAST of Cucsa.094210 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G23340.13.8e-7343.56 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G51310.14.8e-6845.05 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT5G51810.13.4e-2930.50 gibberellin 20 oxidase 2[more]
AT3G19000.14.4e-2926.96 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G21200.17.5e-2928.03 gibberellin 2-oxidase 8[more]
BLAST of Cucsa.094210 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449458351|ref|XP_004146911.1|7.7e-185100.00PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus][more]
gi|659107869|ref|XP_008453897.1|1.0e-16590.06PREDICTED: gibberellin 2-beta-dioxygenase 8-like [Cucumis melo][more]
gi|743828709|ref|XP_011023338.1|4.8e-7849.22PREDICTED: feruloyl CoA ortho-hydroxylase 2-like [Populus euphratica][more]
gi|224067894|ref|XP_002302586.1|1.2e-7648.59hypothetical protein POPTR_0002s16090g [Populus trichocarpa][more]
gi|255541592|ref|XP_002511860.1|1.5e-7648.90PREDICTED: gibberellin 20 oxidase 1 [Ricinus communis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016706 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function GO:0016491 oxidoreductase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.094210.1Cucsa.094210.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 171..265
score: 7.2
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 166..267
score: 11
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 30..73
score: 4.
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 11..305
score: 3.5
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 1..311
score: 6.2E
NoneNo IPR availablePANTHERPTHR10209:SF151SUBFAMILY NOT NAMEDcoord: 1..311
score: 6.2E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 30..305
score: 8.93