CsaV3_4G004090 (gene) Cucumber (Chinese Long) v3 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CsaV3_4G004090
Type	gene
Organism	Cucumis sativus (Cucumber (Chinese Long) v3)
Description	gibberellin 2-beta-dioxygenase 8
Location	chr4 : 2517519 .. 2519186 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAAAATTAAGAGACGAAGAAACGAAGTTTTAACCCAACAGTAATAGTGGAGCTCCCACTGACGCAATTATTGAATGTAAAAAGGAAATCCACAATCAAATCACGTGTAGTAGTATATTCCTAGATCAGCACTATATATCTCAAGAATCAGAATGGTAGTAGTACGAACTATAGCAAGCCAGTTTAATTCCTAAAATTCTAGGGTTTACAGAAATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGTCATCCTAAACTCTATTTCAATCTCAAATCCTCCCGGATTTAATTATTATGATAAAATTACAAATTTGATTAAAGCTTAAGAATCGTTCTATTTAAAATATCTTCGGCAAGTTAAATTTGTTTGCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGAAGTGTTGAAGTATGGACGTTTGGAATGAAAATAGGTATTCATTCTTCTCGTATTAGGATTGGTTGCTGGCTCATGCATAATGCATACATATATGTTATAGTTTTTAAACTTTCAATATGCATATCTCAATTTATGAACTTGGAACAAAAGACAACTATGATGATCTCTTGATGACTTACACATGTACAAGTATAAGTGTATGTAAGATAAAGTTATGTTGGGTTTGCTATACTCGAATCTACCTTATGTGGCTTCAAATATTGCCTATATGTACTTGCATATAGTGGATATACTCAAAAGTATGCTTTAATCATGAAAATATAGAATAGAAAAGGGAAAAAAATTCTCATGGATAGAA

mRNA sequence

ATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGA

Coding sequence (CDS)

ATGTCAAAAGAATCGATTGAGATGCACGAAGAATTACTAGTAATGGACATGAATTCTTGCGAGCATCTGAGCGAATCGAGTCGATTGAGTCTGTACAAGGCGTGTAACGAATGGGGGCATTTCTACATCAAAAACCATGGCGTTTCGAAGGAGCTGTACCAGAAACTCAGAGCCGTTACCGACGAACTGTTGACGGCGGTGCCGGAAGAGACAAAGGAAGGGAAATTGAAAGTAGGAGCTTCTTGGTACACTCCTAGATTTAGATTGTCTCCTTACATTGAGAGCTTCAAGTTTTTGGGCCCGAATTTTTCAGATTATGCCTCCGATTTAGGGTTTACGGAACAAGTCTTCGGCCAACGCGTCACTCAATTCAGGAAACTACTTGATGAATATGGAAGTATAATGATGGAACTATCAAGAAGAATAATGAAGCTATTACTAAAGACGATGGGAGATAATTTGGAGGACAAATTCTACGAATCAGAATTCAGCAACTGCAATGGCTACCTAAGAATAAATAGGTACGCACCTCGAAACTCCAATGAAGAAATCGAAGCATTTGGAAAGCACACTGACATTAGTTGTGTGACGATTATATTCCAAGACGAGATCGGAGGTATTCAAATGAAATGGAAACAAGGAGATGAGTGGGTGGATGTAAGACCTCTAGAGGACGCCCTTTTGGTGAACATTGGGGATTTCTTGCAAGCTTGGAGTAATGAGAGGCTTAGATCAGCAGAACATAGAGTTGTTTTGAAGCAAGATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGATATTTAAGGATGATGATAGGGAGTTATATGCCCCAAGTGAAGTTGTTGGGGAAGGAAATACAAGAATTTATAAGCCATTTTCTACTAAAGAATACAGGGCATATAGGGAAAATAACTACAGGAAAATCGTTGGAGTTCCGCTGAGAGAGTTTGCTGGAATTGATTTGGAGTGA

Protein sequence

MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYRKIVGVPLREFAGIDLE

Homology

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BLAST of CsaV3_4G004090 vs. NCBI nr
Match: XP_004146911.1 (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_4G022900 [Cucumis sativus])

HSP 1 Score: 659.8 bits (1701), Expect = 4.6e-186
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNYRKIVGVPLREFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

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BLAST of CsaV3_4G004090 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo])

HSP 1 Score: 596.3 bits (1536), Expect = 6.3e-167
Identity = 290/322 (90.06%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNY K+VGVP+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

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BLAST of CsaV3_4G004090 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 377.9 bits (969), Expect = 3.5e-101
Identity = 194/312 (62.18%), Postives = 240/312 (76.92%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V+D++  + LSES   SL  ACNEWG FYI NHGVS E YQKL ++ DEL   +P++
Sbjct: 9   ELPVIDLS--QPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF-RLPQD 68

Query: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130
           T   K+KVG S Y PRF  SP+ ESFK  GP+FS  AS    TE + G   TQF  LL+E
Sbjct: 69  T---KIKVGISSYVPRFTASPFFESFKLSGPDFS--ASAQLLTEALLGHHNTQFSNLLEE 128

Query: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKH 190
           YGS M +LS+RI+KLLLK +GDN E+KFY+ EF NC+GYLRIN Y+PR+SNEE+E  GKH
Sbjct: 129 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 188

Query: 191 TDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRV 250
           TDISCVTI+FQD++GG+QM+ K G EW+D+RP E+AL+VNIGDF+QAWSNERLRSAEHRV
Sbjct: 189 TDISCVTILFQDKLGGLQMRSK-GGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRV 248

Query: 251 VLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR---K 310
           VL+Q+VKRFS+AFF  + DD+ E+YAPSEVVGEGN R+Y+ FSTKEYR +R    R   +
Sbjct: 249 VLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYE 308

Query: 311 IVGVPLREFAGI 320
            VG  ++EFAGI
Sbjct: 309 KVGANVKEFAGI 310

[Close]

BLAST of CsaV3_4G004090 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 366.7 bits (940), Expect = 8.1e-98
Identity = 193/318 (60.69%), Postives = 237/318 (74.53%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL V+D+   E LSES+  SL  ACNEWG FYI+NHGVSKE Y +L ++ DEL   +P+E
Sbjct: 9   ELPVIDV--YEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF-RLPQE 68

Query: 71  TKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDE 130
           TK    KVG S Y PRF  SP+ ESFKF GP+FS  AS    TE +F    T+F  LL+E
Sbjct: 69  TKN---KVGISCYVPRFMASPFFESFKFAGPDFS--ASAYSLTEALFDHPNTEFSNLLEE 128

Query: 131 YGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN-----EEIE 190
           YGS M  LS+RI++LLLK + D  E+KFY+SEFSNC G L IN Y+PR+SN     EEIE
Sbjct: 129 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 188

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             GKHTDISCVTI+FQD +GG+QM+ K G EW+D++P EDAL+VNIGDF+QAWSNERLRS
Sbjct: 189 GLGKHTDISCVTILFQDNVGGLQMRSK-GGEWMDIKPCEDALVVNIGDFMQAWSNERLRS 248

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR---- 310
           AEHRVVL+Q+VKRFS++FF  +  DDRE+YAPSEVVGEGN R+Y+PFS KEY+ +R    
Sbjct: 249 AEHRVVLRQNVKRFSMSFFWSYV-DDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSA 308

Query: 311 ENNYRKIVGVPLREFAGI 320
             NY K VG  +++FAGI
Sbjct: 309 RGNYEK-VGATVKDFAGI 315

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BLAST of CsaV3_4G004090 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 366.3 bits (939), Expect = 1.1e-97
Identity = 195/328 (59.45%), Postives = 243/328 (74.09%), Query Frame = 0


Query: 4   ESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDEL 63
           ES     EL V+D+   E LSES+  SL +AC+EWG FYI+NHGVSKE YQKL ++ D+L
Sbjct: 3   ESKSYTVELPVIDV--YEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 62

Query: 64  LTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQ 123
              +P ETK    KVG S Y PRF  SP+ ESFKF GP+FS  A  L  TE + G+   +
Sbjct: 63  F-CLPHETKN---KVGFSCYIPRFIASPFYESFKFSGPDFSASAHHL--TETLLGKHDAE 122

Query: 124 FRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN-- 183
           F  LL EYGS M ++SRRI+KLLLK +GDN E+KFY+SEFSNC+GYLR+N Y+P++SN  
Sbjct: 123 FSNLLKEYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNEN 182

Query: 184 ---EEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAW 243
              EE+E  GKHTDISCVTI+FQD +GG+QM+ K+G EW+D++P EDAL+VNIGDF+ AW
Sbjct: 183 VKIEEVEGLGKHTDISCVTILFQDHVGGLQMRSKEG-EWMDIKPCEDALVVNIGDFMHAW 242

Query: 244 SNERLRSAEHRVVLKQD-VKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEY 303
           SNE+LRSAEHRVVL+Q+ VKRFS+ F   F +DD+E+YAPSEVVGEGN R+YKPF TKEY
Sbjct: 243 SNEKLRSAEHRVVLRQNSVKRFSMVFIWSF-EDDKEIYAPSEVVGEGNARLYKPFLTKEY 302

Query: 304 RAYR------ENNYRKIVGVPLREFAGI 320
           R +R        NY K VG  + +FAGI
Sbjct: 303 RIFRATPRGARENYEK-VGANVTDFAGI 319

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BLAST of CsaV3_4G004090 vs. TAIR10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 277.7 bits (709), Expect = 9.0e-75
Identity = 142/326 (43.56%), Postives = 211/326 (64.72%), Query Frame = 0


Query: 7   EMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 66
           E+H  L +  ++  + +  S   SL +AC EWG FY+ NHG+SKE++ K+ +++ ++  A
Sbjct: 3   ELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVFKA 62

Query: 67  VPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYA---SDLGFTEQVFGQRVTQ 126
                 E KLK+G   YTPR+  SPY ES    GP+FSD A   +D+ F +        +
Sbjct: 63  ----PLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQD----HHKPE 122

Query: 127 FRKLLDEYGSIMMELSRRIMK-LLLKTMGDNLEDKFYESEFSNCNGYLRINRYAP----R 186
            R+ + EYG+ M ELS+R++K LL+ T+GD    + Y+++FSNC+GYLR+  Y P     
Sbjct: 123 LRETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVE 182

Query: 187 NSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAW 246
              E +E  G HTD+SC+TI++QD +GG+QM+ K+G +W+D+ P  D L+VNIGD +QAW
Sbjct: 183 KQEELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEG-KWIDINPCNDFLVVNIGDLMQAW 242

Query: 247 SNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYR 306
           SN RLRS+EHRVVL++ V R SLAFFL F +D++ + AP E+VGEG  R YK F   EY 
Sbjct: 243 SNGRLRSSEHRVVLRKLVNRVSLAFFLCF-EDEKVILAPQEIVGEGKQRSYKSFKCSEYL 302

Query: 307 AYRENNYR---KIVGVPLREFAGIDL 322
            +R++N     + +G  +++FAG+ L
Sbjct: 303 KFRQSNEEGKFEKIGFTVKDFAGLKL 318

[Close]

BLAST of CsaV3_4G004090 vs. TAIR10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 260.8 bits (665), Expect = 1.1e-69
Identity = 150/333 (45.05%), Postives = 210/333 (63.06%), Query Frame = 0


Query: 8   MHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAV 67
           M  EL V D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y+KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVT--QFR 127
            E   E K+K+GAS YTPRF  SP+ ES +  GP+F  YAS     +  F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDF--YASAKSSVD-AFSDQATDEEFS 120

Query: 128 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L + GD+L  K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++ + G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQD---VKRFSLAFFLIFKDDDRELYAPSEVVG--EGNTRIYK 307
            AW+N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAYRENNYR---KIVGVPLREFAGID 321
            F   +Y  +RE+N +   + VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of CsaV3_4G004090 vs. TAIR10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 130.2 bits (326), Expect = 2.3e-30
Identity = 86/319 (26.96%), Postives = 153/319 (47.96%), Query Frame = 0


Query: 10  EELLVMDMNSCE---HLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTA 69
           +E+  +D++S E   H   +    + +AC  WG F + NHG+   L  ++     E    
Sbjct: 30  DEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 89

Query: 70  VPEETKEGKL-KVGASWYTPR---FRLSPYIESFKFL---------GPNFSDYASDLGFT 129
             EE ++ K  +V    Y        +  + E F F           P   D  ++L   
Sbjct: 90  TTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPED--TELRKL 149

Query: 130 EQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL---LKTMGDNLEDKFYESEFSNCNGYL 189
              + Q  + FR++  EY   + +L+ R+++L+   L   GD L   F E        +L
Sbjct: 150 TNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQ-----TSFL 209

Query: 190 RINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVN 249
           R N Y P  + E     G+H D   +T++ QD +GG+Q+  +   +W+ V+P+ DAL++N
Sbjct: 210 RFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIIN 269

Query: 250 IGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYK 309
           +G+ +Q W+N+   SAEHRVV+    +RFS+ FF  F   +  +    E++ E N   YK
Sbjct: 270 MGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFF-FFPSHEANIEPLEELISEENPPCYK 329

[Close]

BLAST of CsaV3_4G004090 vs. TAIR10
Match: AT4G21200.1 (gibberellin 2-oxidase 8)

HSP 1 Score: 129.8 bits (325), Expect = 3.0e-30
Identity = 88/314 (28.03%), Postives = 151/314 (48.09%), Query Frame = 0


Query: 11  ELLVMD----MNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELL-T 70
           EL V+D    ++  E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEETKEGKLKVGA-SWYTP---RFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TI++QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]

BLAST of CsaV3_4G004090 vs. TAIR10
Match: AT5G51810.1 (gibberellin 20 oxidase 2)

HSP 1 Score: 129.4 bits (324), Expect = 4.0e-30
Identity = 97/318 (30.50%), Postives = 158/318 (49.69%), Query Frame = 0


Query: 3   KESIEMHE-ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTD 62
           K SI++ E  +  +D++S +   E+ R+ + +AC + G F + NHGVS+ L      + +
Sbjct: 53  KPSIDIPELNVPFIDLSSQDSTLEAPRV-IAEACTKHGFFLVVNHGVSESLIADAHRLME 112

Query: 63  ELLTAVPEETKEGKLKVG-----ASWYTPRFRLS-PYIE--SFKFLGPN-----FSDYAS 122
                     ++ + K G     AS +T RF    P+ E  SF+F   N       DY S
Sbjct: 113 SFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFS 172

Query: 123 DLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNG 182
           D        GQ   QF K+  +Y   M  LS +IM+LL  ++G N +  ++   F   + 
Sbjct: 173 D------TLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD--YFRGFFEENDS 232

Query: 183 YLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL 242
            +R+N Y P  + +     G H D S +TI+ QD + G+Q+     ++W  +RP   A +
Sbjct: 233 IMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQV--FVDNQWQSIRPNPKAFV 292

Query: 243 VNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRI 302
           VNIGD   A SN   +S  HR V+ ++  R S+AFFL  K  D+ +  PS+++ +  TR 
Sbjct: 293 VNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK-KDKVVKPPSDILEKMKTRK 352

Query: 303 YKPFSTKEYRAYRENNYR 307
           Y  F+   +  + + +YR
Sbjct: 353 YPDFTWSMFLEFTQKHYR 358

[Close]

BLAST of CsaV3_4G004090 vs. Swiss-Prot
Match: sp|F4KBY0|GAOXL_ARATH (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-68
Identity = 150/333 (45.05%), Postives = 210/333 (63.06%), Query Frame = 0


Query: 8   MHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAV 67
           M  EL V D++  + LSESS  SL  AC EWG FY+ NHGVS+++Y+KLR  +    T V
Sbjct: 1   MSLELPVFDIS--KPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFS----TGV 60

Query: 68  PEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVT--QFR 127
            E   E K+K+GAS YTPRF  SP+ ES +  GP+F  YAS     +  F  + T  +F 
Sbjct: 61  FELEDEEKMKMGASNYTPRFIASPFFESLRVSGPDF--YASAKSSVD-AFSDQATDEEFS 120

Query: 128 KLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEE-- 187
            L+ EYG  M +L  +IMK +L + GD+L  K+YESEF NC+GY RIN Y   +  E+  
Sbjct: 121 GLMKEYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDH 180

Query: 188 --------IEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFL 247
                   IE  G HTD+SC+TI+ QD+IGG+Q++ + G   +D+ P ++AL+VN+GD L
Sbjct: 181 HNGDEQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLL 240

Query: 248 QAWSNERLRSAEHRVVLKQD---VKRFSLAFFLIFKDDDRELYAPSEVVG--EGNTRIYK 307
            AW+N RLRS++HRV+LK+      RFSLAFF  F DD + ++AP EVVG  EG  R+++
Sbjct: 241 HAWTNGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFR 300

Query: 308 PFSTKEYRAYRENNYR---KIVGVPLREFAGID 321
            F   +Y  +RE+N +   + VG  + +FA I+
Sbjct: 301 SFKCGDYLRFRESNEKGKFEKVGDTVEDFARIE 322

[Close]

BLAST of CsaV3_4G004090 vs. Swiss-Prot
Match: sp|O49561|G2OX8_ARATH (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 129.8 bits (325), Expect = 5.5e-29
Identity = 88/314 (28.03%), Postives = 151/314 (48.09%), Query Frame = 0


Query: 11  ELLVMD----MNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELL-T 70
           EL V+D    ++  E   E  + ++ +A  EWG F + NHG+S ++ +K+R     +   
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  AVPEETKEGKLKVGA-SWYTP---RFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRV 130
              +++K  K   G+  W TP     R   + E+F     + SD             +  
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISD------------NKDF 160

Query: 131 TQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSN 190
           T     ++++ S    L+  + ++L +  G N    F++        YLR+NRY P    
Sbjct: 161 TTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKP 220

Query: 191 EEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNE 250
            E+     HTD   +TI++QD++GG+Q+   + + W+ V+P   AL++NIGD  QAWSN 
Sbjct: 221 SEVYGLMPHTDSDFLTILYQDQVGGLQL--IKDNRWIAVKPNPKALIINIGDLFQAWSNG 280

Query: 251 RLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYR 310
             +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+R   
Sbjct: 281 MYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREFRQQV 331

Query: 311 ENNYRKI---VGVP 313
           + + +K    VG+P
Sbjct: 341 QEDVKKFGFKVGLP 331

[Close]

BLAST of CsaV3_4G004090 vs. Swiss-Prot
Match: sp|Q39111|GAOX2_ARATH (Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.1e-29
Identity = 97/318 (30.50%), Postives = 158/318 (49.69%), Query Frame = 0


Query: 3   KESIEMHE-ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTD 62
           K SI++ E  +  +D++S +   E+ R+ + +AC + G F + NHGVS+ L      + +
Sbjct: 53  KPSIDIPELNVPFIDLSSQDSTLEAPRV-IAEACTKHGFFLVVNHGVSESLIADAHRLME 112

Query: 63  ELLTAVPEETKEGKLKVG-----ASWYTPRFRLS-PYIE--SFKFLGPN-----FSDYAS 122
                     ++ + K G     AS +T RF    P+ E  SF+F   N       DY S
Sbjct: 113 SFFDMPLAGKQKAQRKPGESCGYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFS 172

Query: 123 DLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNG 182
           D        GQ   QF K+  +Y   M  LS +IM+LL  ++G N +  ++   F   + 
Sbjct: 173 D------TLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRD--YFRGFFEENDS 232

Query: 183 YLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALL 242
            +R+N Y P  + +     G H D S +TI+ QD + G+Q+     ++W  +RP   A +
Sbjct: 233 IMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQV--FVDNQWQSIRPNPKAFV 292

Query: 243 VNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRI 302
           VNIGD   A SN   +S  HR V+ ++  R S+AFFL  K  D+ +  PS+++ +  TR 
Sbjct: 293 VNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPK-KDKVVKPPSDILEKMKTRK 352

Query: 303 YKPFSTKEYRAYRENNYR 307
           Y  F+   +  + + +YR
Sbjct: 353 YPDFTWSMFLEFTQKHYR 358

[Close]

BLAST of CsaV3_4G004090 vs. Swiss-Prot
Match: sp|Q39110|GAOX1_ARATH (Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 3.9e-27
Identity = 90/292 (30.82%), Postives = 141/292 (48.29%), Query Frame = 0


Query: 25  ESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKEGKLKVG----- 84
           ++SRL + +AC + G F + NHG+S+EL       T         E +    K G     
Sbjct: 78  DASRL-ISEACKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGY 137

Query: 85  ASWYTPRFRLS-PYIESFKFLGPNFSDYASDLGFTEQVF----GQRVTQFRKLLDEYGSI 144
           AS +T RF    P+ E+  F    F D  S     +  F    G     F K+  EY   
Sbjct: 138 ASSFTGRFSTKLPWKETLSF---RFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEA 197

Query: 145 MMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDIS 204
           M  LS +IM+LL  ++G  ++  ++   F   +  +R+N Y P    +     G H D +
Sbjct: 198 MSSLSLKIMELLGLSLG--VKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPT 257

Query: 205 CVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQ 264
            +TI+ QD + G+Q+  +  ++W  +RP   A +VNIGD   A SN+R +S  HR V+  
Sbjct: 258 SLTILHQDHVNGLQVFVE--NQWRSIRPNPKAFVVNIGDTFMALSNDRYKSCLHRAVVNS 317

Query: 265 DVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNYR 307
           + +R SLAFFL  K  DR +  P E++    +R Y  F+   +  + + +YR
Sbjct: 318 ESERKSLAFFLCPK-KDRVVTPPRELLDSITSRRYPDFTWSMFLEFTQKHYR 360

[Close]

BLAST of CsaV3_4G004090 vs. Swiss-Prot
Match: sp|Q7XP65|G2OX6_ORYSJ (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 6.7e-27
Identity = 89/295 (30.17%), Postives = 137/295 (46.44%), Query Frame = 0


Query: 30  SLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEETKE--GKLKVGASWYTPRF 89
           ++ +A +EWG F + NHGV +EL +++R     L   +P ETKE  G L     W  P  
Sbjct: 72  AMARAASEWGFFQLTNHGVGRELMEEMRREQARLF-RLPFETKEKAGLLNGSYRWGNPTA 131

Query: 90  RLSPYIE-SFKFLGPNFSDYASDLGFTEQVFGQRVTQFRKLLDEYGSIMMELSRRIMKLL 149
               ++  S  F  P  S   +D  F +      +T  R ++ E    M  ++  +   L
Sbjct: 132 TSLRHLSWSEAFHVPLASISGADCDFGD------LTSLRGVMQEVAEAMSRVANTVAAAL 191

Query: 150 LKTM-----GDNLEDKFYESEFSNCNGYLRINRYAPRNSNEEIEAFGKHTDISCVTIIFQ 209
            + +     G      ++ +       +LR+NRY       +      HTD   +T++ Q
Sbjct: 192 AEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQ 251

Query: 210 DEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRSAEHRVVLKQDVKRFSL 269
           D++GG+ +   +   WV VRP  DAL+VNIGD  QAWSN R +S EH+VV      R S+
Sbjct: 252 DQVGGLHL--MKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSV 311

Query: 270 AFFLIFKDDDRELYAPSEVVGE-GNTRIYKPFSTKEYRAYRENNYR---KIVGVP 313
           A+FL    D         +VG  G    Y+ F+  EYR   + + R   K +G+P
Sbjct: 312 AYFLCPSYD--------SLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLP 349

[Close]

BLAST of CsaV3_4G004090 vs. TrEMBL
Match: tr|A0A0A0KW97|A0A0A0KW97_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 3.1e-186
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNYRKIVGVPLREFAGIDLE
Sbjct: 301 RENNYRKIVGVPLREFAGIDLE 322

[Close]

BLAST of CsaV3_4G004090 vs. TrEMBL
Match: tr|A0A1S3BYJ4|A0A1S3BYJ4_CUCME (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 4.2e-167
Identity = 290/322 (90.06%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60
           MSKESIEM EEL VMDMN CEHLSESSRLSLYKACNEWGHFYIKNHGVSKELY KLRAVT
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120
           DELLTAVPEE KE KLKVG SWYTPRF+LSPY ESFKFLGP FSD ASDLGFTEQVFG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180
           VTQFR LLDEYGSIM+ELSRRIMKLLLK MGD+ EDKFYESEFSNCNGY+RINRYAPRNS
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240
           NEEIEAFGKHTDISCVTI+FQDEIGGIQMK KQGDEWVDVRPLEDALLVNIGDFLQAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300
           ERLRSAEHRVVLKQ+ KRF+L FFL+F DDDRELYAPSEVVGEGN R+YKPFSTKEYRA+
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RENNYRKIVGVPLREFAGIDLE 323
           RENNY K+VGVP+REFAGIDLE
Sbjct: 301 RENNYGKVVGVPMREFAGIDLE 322

[Close]

BLAST of CsaV3_4G004090 vs. TrEMBL
Match: tr|B9GQ95|B9GQ95_POPTR (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G159500v3 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.1e-78
Identity = 155/319 (48.59%), Postives = 224/319 (70.22%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + L  S+  SL +AC EWG F I NHG+SKELY KL +++ +L   +P E
Sbjct: 8   ELPILDIS--QPLQPSALASLAEACQEWGFFRISNHGISKELYNKLYSLSQQLF-GLPAE 67

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           T   KL++G S     YTP F  SP+ E  +  GPNF  +AS     + +FGQ+  +FR 
Sbjct: 68  T---KLELGPSSSINTYTPHFIASPFFECLRVFGPNF--FASAQSSADTLFGQQSFEFRA 127

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRY-APRNSNEEIE 190
            L EYG+ M ELS+RI+++L  ++G+  ++K+YESEF NC+GYLRI  Y  P+   +E+E
Sbjct: 128 ALQEYGTKMTELSKRIVEILSMSLGEGFDNKYYESEFKNCHGYLRIINYNPPKGLVDEVE 187

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDE+GG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSN++LRS
Sbjct: 188 GLGMHTDMSCVTIVYQDEVGGLQVKSREG-KWMDISPGEETLVVNIGDLLQAWSNDKLRS 247

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EHR+VLK+ V R SLAFF  F +D++ + AP+EVVGEGN RIY+PF   +Y  +RE++ 
Sbjct: 248 SEHRIVLKKPVNRLSLAFFWCF-EDEKVIMAPNEVVGEGNARIYEPFVCSDYLKFRESSE 307

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAGI L
Sbjct: 308 RGKFEKVGFTVKDFAGISL 316

[Close]

BLAST of CsaV3_4G004090 vs. TrEMBL
Match: tr|B9RDS1|B9RDS1_RICCO (Gibberellin 20-oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_1615250 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.8e-78
Identity = 156/319 (48.90%), Postives = 222/319 (69.59%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + +++SS  SL +AC EWG F I NHG+SK+LY K+  ++  +  ++P E
Sbjct: 9   ELPILDIS--KPINQSSLSSLAEACKEWGFFNITNHGISKQLYNKIYLLSQNIF-SLPSE 68

Query: 71  TKEGKLKVGAS----WYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
               K K+G S     YTP F  SPY ES +  GPNF   AS     + +F Q+ ++F  
Sbjct: 69  I---KFKLGPSSSIKTYTPHFIASPYFESLRVSGPNF--LASAQSSADVLFNQQNSEFSD 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYA-PRNSNEEIE 190
           +L EYGS M ELS+RI+++LL ++GD L+ KFYE+EF+NC+GYLRI  Y  P+N  +E+E
Sbjct: 129 VLQEYGSKMTELSKRIVEMLLLSLGDGLDKKFYETEFNNCHGYLRIVNYTPPKNLEDEVE 188

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SC+TI++QDEIGG+Q+K ++G +W+D+ P E+ L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCITIVYQDEIGGLQVKSREG-KWMDISPCEETLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EH+VVLKQ   R SLAFF  F +D + + AP EVVGEG  RIYKPF   +Y  +RE+N 
Sbjct: 249 SEHQVVLKQSANRLSLAFFWCF-EDQKVISAPDEVVGEGCGRIYKPFVCSDYLKFRESNE 308

Query: 311 R---KIVGVPLREFAGIDL 322
           R   + VG  +++FAG++L
Sbjct: 309 RGKFEKVGFTVKDFAGLNL 317

[Close]

BLAST of CsaV3_4G004090 vs. TrEMBL
Match: tr|A0A2N9FI94|A0A2N9FI94_FAGSY (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14707 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 6.2e-78
Identity = 159/320 (49.69%), Postives = 219/320 (68.44%), Query Frame = 0


Query: 11  ELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVTDELLTAVPEE 70
           EL ++D++  + L  S   SL +AC +WG F+I NHG+SK+L+ KL  ++ +L   +P E
Sbjct: 9   ELPILDIS--QPLHPSFLTSLAEACKKWGFFHIINHGISKDLFSKLYLLSKDLFN-LPSE 68

Query: 71  TKEGKLKVG----ASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQRVTQFRK 130
           T   KLKVG       YTP F  SPY ES +  GPNF  +AS       +F Q+ ++F +
Sbjct: 69  T---KLKVGPFSSIKTYTPHFIASPYFESLRVSGPNF--FASAQSSANALFVQQNSEFSE 128

Query: 131 LLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRY-APRNSNEEIE 190
           +L EYG  M ELS++I++ LL ++ D  E K+YESEF NC+GYLRIN Y AP +  +E+E
Sbjct: 129 ILQEYGRKMEELSKKIIEALLISLEDGFEKKYYESEFKNCDGYLRINNYLAPESLEDEVE 188

Query: 191 AFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSNERLRS 250
             G HTD+SCVTI++QDEIGG+Q++ K+G +W+D+RP E  L+VNIGD LQAWSNE+ RS
Sbjct: 189 GLGMHTDMSCVTIVYQDEIGGLQVRSKEG-KWMDIRPCEGTLVVNIGDMLQAWSNEKFRS 248

Query: 251 AEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAYRENNY 310
           +EHRVVLKQ   RFSLAFF  F +D++ + AP +VV EGN R+Y PF   +Y  +RE+N 
Sbjct: 249 SEHRVVLKQPANRFSLAFFWCF-EDEKVILAPDDVVREGNVRLYNPFVCLDYLKFRESNE 308

Query: 311 R---KIVGVPLREFAGIDLE 323
           R   + VG  +R+FAGI L+
Sbjct: 309 RGKFEKVGFTVRDFAGIGLK 318

The following BLAST results are available for this feature:

BLAST of CsaV3_4G004090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146911.1	4.6e-186	100.00	PREDICTED: gibberellin 20 oxidase 2-like [Cucumis sativus] >KGN53154.1 hypotheti...	[more]
XP_008453897.1	6.3e-167	90.06	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo]	[more]
XP_022141254.1	3.5e-101	62.18	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]
XP_022141253.1	8.1e-98	60.69	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022140867.1	1.1e-97	59.45	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]

BLAST of CsaV3_4G004090 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23340.1	9.0e-75	43.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.1e-69	45.05	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	2.3e-30	26.96	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G21200.1	3.0e-30	28.03	gibberellin 2-oxidase 8	[more]
AT5G51810.1	4.0e-30	30.50	gibberellin 20 oxidase 2	[more]

BLAST of CsaV3_4G004090 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|F4KBY0\|GAOXL_ARATH	2.1e-68	45.05	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
sp\|O49561\|G2OX8_ARATH	5.5e-29	28.03	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
sp\|Q39111\|GAOX2_ARATH	7.1e-29	30.50	Gibberellin 20 oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=GA20OX2 PE=2 SV=1	[more]
sp\|Q39110\|GAOX1_ARATH	3.9e-27	30.82	Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=GA20OX1 PE=2 SV=2	[more]
sp\|Q7XP65\|G2OX6_ORYSJ	6.7e-27	30.17	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of CsaV3_4G004090 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0KW97\|A0A0A0KW97_CUCSA	3.1e-186	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1	[more]
tr\|A0A1S3BYJ4\|A0A1S3BYJ4_CUCME	4.2e-167	90.06	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
tr\|B9GQ95\|B9GQ95_POPTR	2.1e-78	48.59	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_002G159500v3 PE=...	[more]
tr\|B9RDS1\|B9RDS1_RICCO	2.8e-78	48.90	Gibberellin 20-oxidase, putative OS=Ricinus communis OX=3988 GN=RCOM_1615250 PE=...	[more]
tr\|A0A2N9FI94\|A0A2N9FI94_FAGSY	6.2e-78	49.69	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS14707 PE=3 SV=1	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR026992	DIOX_N
IPR027443	IPNS-like

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0051213	dioxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_4G004090.1	CsaV3_4G004090.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber chineselong genome (v3)
Date Performed: 2019-03-04


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 10..311 e-value: 1.9E-62 score: 213.4
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..73 e-value: 3.8E-9 score: 37.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..265 e-value: 9.4E-21 score: 74.1
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 166..267 score: 11.145
None	No IPR available	PANTHER	PTHR10209:SF212	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 7..308
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 7..308
None	No IPR available	SUPERFAMILY	SSF51197	Clavaminate synthase-like	coord: 30..305

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CsaV3_4G004090	CSPI04G04030	Wild cucumber (PI 183967)	cpicucB213
CsaV3_4G004090	Cucsa.094210	Cucumber (Gy14) v1	cgycucB102
CsaV3_4G004090	CsGy4G003950	Cucumber (Gy14) v2	cgybcucB171
CsaV3_4G004090	Cla010726	Watermelon (97103) v1	cucwmB324
CsaV3_4G004090	ClCG07G016880	Watermelon (Charleston Gray)	cucwcgB336
CsaV3_4G004090	Lsi07G001050	Bottle gourd (USVL1VR-Ls)	cuclsiB322
CsaV3_4G004090	MELO3C032832.2	Melon (DHL92) v3.6.1	cucmedB307
CsaV3_4G004090	Cla97C07G143880	Watermelon (97103) v2	cucwmbB324
CsaV3_4G004090	Bhi01G002032	Wax gourd	cucwgoB373

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CsaV3_4G004090	Silver-seed gourd	carcucB0405
CsaV3_4G004090	Silver-seed gourd	carcucB0910
CsaV3_4G004090	Cucurbita maxima (Rimu)	cmacucB0387
CsaV3_4G004090	Cucurbita maxima (Rimu)	cmacucB0842
CsaV3_4G004090	Cucurbita moschata (Rifu)	cmocucB0384
CsaV3_4G004090	Cucurbita moschata (Rifu)	cmocucB0828
CsaV3_4G004090	Cucurbita pepo (Zucchini)	cpecucB0262
CsaV3_4G004090	Cucurbita pepo (Zucchini)	cpecucB0498
CsaV3_4G004090	Melon (DHL92) v3.5.1	cucmeB315